JP2007508309A - Coated metal pyrithione particles for treating microorganisms - Google Patents

Abstract

A composition comprising coated particles of a salt of pyrithione selected from the group consisting of monovalent and polyvalent pyrithione salts, and combinations thereof, partially or fully coated with a lipophilic agent. The composition is characterized by enhanced anti-fungal and anti-dandruff efficacy in shampoos. Also disclosed is a personal care composition, preferably a shampoo, containing a topical carrier and coated particles of a salt of pyrithione selected from the group consisting of monovalent and polyvalent pyrithione salts, and combinations thereof, partially or fully coated with a lipophilic agent.

Description

  The present invention relates to coated pyrithione salt particles, wherein the coating improves the efficacy of the pyrithione moiety, thereby increasing the duration of the antimicrobial efficacy provided by the composition. The composition is useful for providing antibacterial, especially antifungal efficacy, when formulated in human care compositions, particularly shampoos.

  In shampoos, the lipophilicity of the coating makes it easier to achieve targeted delivery to the scalp, particularly to sites of the scalp that contain lipophilic microorganisms such as lipophilic yeasts known to cause dandruff. Shampoos comprising the coated particles of the present invention exhibit increased antifungal efficacy compared to shampoos comprising uncoated pyrithione salt particles.

  Metal pyrithiones such as zinc and copper pyrithione are well known as effective antimicrobial agents. Zinc pyrithione is well known to be active as a shampoo anti-dandruff and antifungal component and is widely used.

  PTC patent application WO 03/007900 describes the use of lipophilic agents such as ceramide with zinc pyrithione to increase the level of lipids in the skin and scalp to treat dandruff. However, the use of a mixture of lipid and pyrithione salt in a shampoo formulation has the disadvantage of preferentially reaching the lipid to one location on the scalp and delivering the pyrithione salt to another location. .

  The present invention addresses the above disadvantages by using a lipid coating on the pyrithione particles to facilitate targeted delivery of the lipid and pyrithione as a single package.

  In one embodiment, the present invention comprises coated particles of pyrithione salts selected from the group consisting of monovalent and polyvalent pyrithione salts, and combinations thereof, partially or fully coated with a lipophilic agent. Relates to the composition. This composition provides shampoos with increased antifungal and antidandruff efficacy.

  In another aspect, the invention provides a pyrithione salt selected from the group consisting of topical carriers and mono- and polyvalent pyrithione salts, and combinations thereof, partially or fully coated with a lipophilic agent. It relates to a human care composition comprising coated particles, preferably a shampoo.

In yet another aspect, the present invention provides a method for producing an aqueous dispersion of solid zinc pyrithione particles having solid palm oil coating on its outer surface with solid zinc pyrithione particles.
(A) heating an aqueous dispersion of solid zinc pyrithione particles to an elevated temperature of about 50 ° C. to about 80 ° C. to provide a high temperature aqueous dispersion;
(B) heating solid palm oil to an elevated temperature of about 50 ° C. to about 80 ° C. to provide liquid palm oil; and (c) mixing the liquid palm oil with the high temperature aqueous dispersion. Providing a mixture and cooling the mixture to provide an aqueous dispersion of solid zinc pyrithione particles having the solid palm oil coating;
It relates to the manufacturing method containing. The resulting dispersion contains suitably coated zinc pyrithione particles in an amount of about 40% to about 50%, based on the total weight of the dispersion.

  In yet another aspect, the present invention relates to an aqueous antimicrobial dispersion concentrate comprising water and about 25 wt% to about 50 wt% solid pyrithione salt particles, based on the total weight of the dispersion. Solid pyrithione salt particles are coated particles having a lipophilic coating on their outer surface. The coated particles have at least 25% antimicrobial efficacy in a comparative corneofungimetry bioassay test compared to the antimicrobial efficacy in the bioassay test of the dispersion of the uncoated particles of pyrithione salt. Indicates an improvement. Another aspect relates to a human care composition comprising a topical carrier and from about 0.5% to about 5% of the concentrate, based on the total weight of the person's care composition. The human care composition is compared with the antimicrobial efficacy of the human care composition containing the same amount of the uncoated particles of pyrithione salt in the comparative corneophanemetry bioassay test. An antibacterial efficacy improvement of at least 15%.

  These and other aspects will become apparent upon reading the following detailed description of the invention.

Detailed Description of the Invention It has now been unexpectedly discovered that coated pyrithione particles comprising a lipophilic fatty oil coating provide an effective vehicle for delivering pyrithione to the desired lipophilic site of the scalp. At these sites there is a tendency to find lipophilic microorganisms such as lipophilic Malassezia sp yeast that are known to cause dandruff. Delivery targeted to these sites increases the antifungal and anti-dandruff efficacy of shampoos containing these coated pyrithione particles compared to shampoos containing uncoated pyrithione particles.

  The pyrithione particles used in the present invention are preferably selected from the group consisting of zinc, copper, silver, zirconium salts, and combinations thereof. More preferred pyrithione salts are selected from zinc and copper salts, most preferably zinc salts. Zinc salts are suitably used in the form of granular dispersions, slurries or powders. Zinc pyrithione may be used in any particle form, including, for example, crystalline forms such as platelets, rods, needles, blocks, spheres, and amorphous, regular or irregular shaped particles. . The average particle diameter (maximum dimension) of the zinc pyrithione particles is typically about 0.1 to about 50 μm, as determined using, for example, a Horiba LA-910 laser scattering particle size distribution analyzer, The thickness is preferably about 0.1 μm to about 10 μm, more preferably about 0.1 μm to about 5 μm.

  The lipophilic coating on the coated particles of the present invention comprises a synthetic or natural fatty oil that is solid or liquid at room temperature. Thus, as used herein, the term “lipophilic agent” is intended to encompass both oils and waxes. The fatty oil comprises a fatty acid or fatty acid ester having a number average molecular weight in the range of about 150 to about 1500 and a carbon number in the range of 8 to 22, or mixtures thereof. Fatty acid esters can include mono-, di-, or tri-glycerides, or mixtures thereof. A preferred coating material is palm oil.

  The proportion occupied by the lipophilic coating component is preferably greater than 0.01% by weight, preferably greater than 1% by weight, more preferably greater than 3% by weight, based on the total weight of the coated pyrithione particles. Typically, the lipophilic agent content in the coated particles is in the range of about 3-15%. For shampoos containing 1% by weight zinc pyrithione, the amount of lipophilic coating agent is generally from about 0.001% to about 0.15% by weight, preferably from about 0.01% to about 0.1%, based on the weight of the shampoo. About 0.15 wt%, more preferably about 0.03 wt% to about 0.15 wt%.

  Suitable fatty acids and fatty acid esters include hydrocarbon oils, waxes, di- and triglyceride oils, such as petrolatum, mineral oil wax, hydrogenated and non-hydrogenated polyalkenes, hydrogenated oils, paraffins, synthetic paraffins. Polyethylene and polybutene palm oil, castor oil, soybean oil, derivatized soybean oil, such as sunflower oil, cottonseed oil, corn oil, walnut oil, peanut oil, sesame oil, vegetable oil, vegetable oil derivative, coconut oil, coconut oil derivative.

  The fatty acid / fatty acid ester can be naturally occurring or derivatives thereof. The carbon chain length of the coating is generally C8-C22 and includes mixtures thereof. The fatty acids can be saturated and unsaturated, linear and branched chain fatty acids. They are selected based on the chemical and physical properties of the fatty acids and their derivatives.

  Suitable natural and synthetic sources of fatty acids and their derivatives include those of animal and plant origin, such as lanolin oil, fish oil, animal oil, coconut oil, palm oil, butterfat, soybean oil, sunflower oil, cottonseed oil, corn oil And mixtures thereof. Preferred fatty acid sources are palm oil, partially and fully hydrogenated fatty oils, fatty acids such as hydrogenated soybean oil, palm oil, palm kernel oil, fish oil, polyol fatty acid esters.

  As an alternative, the lipophilic coating is solid at room temperature, becomes liquid at elevated temperatures, and cools to form a film. Desirably, the solid coating material becomes liquid at about 40-100 ° C. and can form a film during subsequent cooling. Alternatively, the lipophilic coating is suitably dissolved in a solvent and dried to form a film. By way of example, the coating is suitably dissolved in an organic solvent, for example an alkyl alcohol such as methanol and ethanol, acetone, ether, halogenated hydrocarbons, and aromatic solvents, and then the film is evaporated while the solvent is evaporated. Form.

  In the present invention, for particle coating in water, a slurry of a coating or a suspended metal pyrithione or pyrithione particle under the condition that the coating material is in a liquid state or a solution state. A polyvalent salt coating is included. The size, shape, and thickness of the coated particles depend on the size and shape of the original particles in the slurry or suspension, the temperature, and the amount of coating material used. This method involves adding a coating material in a liquid state (preferably at a melting temperature below about 90 ° C.) to the slurry or suspension at a temperature at which the coating material is in a liquid state with stirring at an appropriate rate. And then briefly described as a mixture of the metal pyrithione slurry and coating component, or a mixture of coating materials, or heated to the highest melting temperature of the coating material used and then cooled. it can.

  Particle coating in an organic solvent involves adding a metal pyrithione or polyvalent salt of pyrithione powder to a solution of the coating material (one or many) in an organic solvent at a temperature above room temperature, and then stirring the solvent while stirring. Includes evaporation. The size, shape, and thickness of the coated particles depend on the original particle size, the shape of the metal pyrithione, and the amount and nature of the coating material.

  A powder coating can also be suitably used to coat pyrithione at temperatures above the melting point of the polymer coating material. The liquid coating material forms a film or coating layer on the particles while cooling. The size, shape, and coating thickness of the amount coated depends on the original size and shape of the metal pyrithione particles and the amount and nature of the coating material used. This method is particularly suitable for coating particles with materials having a low (preferably less than about 90 ° C.) melting point.

  The coated pyrithione particles of the present invention are suitable for use in human care compositions containing a topical carrier. The topical carrier is suitably selected from a wide range of conventional human care carriers, depending on the type of composition being formed. By appropriately selecting a compatible carrier, forms of daily skin or hair products such as hair rinses, daily hair care products such as hair lotions, hair sprays, hair tonics, conditioning treatments, and dressings, etc. Thus, it is considered that the antibacterial composition of the present invention can be prepared.

  The topical carrier in the liquid hair composition can be water, a common organic solvent, or a mixture thereof. Suitable common organic solvents are C2-C3 alkyl alcohols such as ethanol, propanol, isopropanol, glycerin. Other solvents such as fatty alcohols and esters can also be used.

  The antimicrobial composition of the present invention may be an aqueous system comprising about 40% to about 92%, preferably about 50% to about 85%, more preferably about 60% to about 80% water by weight of the composition. .

  When the composition of the present invention is an anti-dandruff shampoo, the pH of the composition is generally about 2 to about 10, preferably about 3 to about 9, more preferably about 4 to about 8, and most preferably about 5.5. Is in the range of ~ 7.5.

  The following examples are intended to illustrate the present invention and are not intended to limit its scope in any way. All coated particles were characterized by ESCA, SEM, and particle size distribution analyzer, and the content of zinc pyrithione in the coated particle size distribution was determined using standard titration methods.

Example 1
Zinc pyrithione powder (about 200 g) having a median particle size of about 3.4μ was placed in a small granulator. Palm oil (about 24 g) was melted on a hot plate and placed in a heated feed container maintained at 70 ° C. The two fluid nozzles were heated and maintained at 75 ° C. The granulator was started and the molten coating material was sprayed into the stirred powder.

The coated particles had the following characteristics:
Analysis value of zinc pyrithione: 85.8%
Particle size [Horiba (Horriba) LA-910]: median _(D 50): _7.44μm; specific surface area: after 2 min sonication, 17647cm ² / ^cm 3.

The coating thickness derived from the ESCA analysis of the atomic concentration and the decay of the Zn and S signals showed that the presence of the coating was 35 mm.
The SEM image of the coated particles showed that the particles were visually coated and had cluster characteristics.

Comparative Example A:
D. of ~ 65 ° C. I. To a stirred suspension of 210.0 g of 48.0% zinc pyrithione (median particle size 0.47 μm) in 150 ml of water was slowly added hot liquid oil consisting of 4.1 g of stearyl dimethicone at 60-65 ° C. . After stirring at 500 rpm for 10 minutes at this temperature, the dispersion was gradually cooled to room temperature with a stirring speed of 300 rpm.
The coated particles had the following characteristics:
Analysis value of zinc pyrithione: 32.0%
Particle size (Horiba LA-910): Median (D ₅₀ ): 0.478 μm; Average: 0.507 μm; Specific surface area: 136811 cm ² / cm ³ after sonication for 2 minutes.

  The coating thickness derived from the ESCA analysis of atomic concentration and the decay of the Zn and S signals indicated that the presence of the coating was 15 mm, as shown in Table 1. The SEM image of the coated particles showed that the particles were thinly coated. Zinc pyrithione particles (0.1-1 μm, a mixture of blocks and platelets) had some scattered coated patches, had a well-defined profile, and the edges of the particles were apparently softened. As seen for the fine zinc pyrithione particles, these coating-related features with curved features rather than straight or angular features are part of the original fine particles, as seen with ESCA May be just a thin coating.

  The coated particles showed activity in a Qualitative Zone of Inhibition (ZOI) test against Malassezia furfur, demonstrating that the coating did not block the migration of zinc pyrithione through the agar.

Comparative Examples B to D:
EXAMPLE 1 Zinc pyrithione particles placed in a suspension of 48.0% zinc pyrithione (median particle size 0.47 μm) according to the procedure of Example 1- (I) were converted to aminofunctional polydimethylsiloxane [Amodimethicon. ), Dow Corning 2-8566 amino fluid, Example B], lecithin [Centromix LP250, Example C], and bis-PEG-12 dimethicone beeswax (Siliconyl beeswax, Example D). did. The characteristic data of these coated zinc pyrithione particles are listed in Table 1.

  Initial performance tests demonstrated that Example 1 and all of the comparative examples showed coated particle antimicrobial activity in a qualitative zone of inhibition (ZOI) test against Malassezia furfur and the coating did not block the migration of zinc pyrithione. Was.

  As a further test, the particles prepared according to the examples and comparative examples were evaluated using “Corneophanimetry”. This test is a good preclinical screening method. This is because it requires the growth of Malassezia furfur sp on a substrate that better represents the natural environment. Cyanoacrylate is used to collect the skin layer, and these skin surface exfoliants are treated with olive oil to resemble sebum. The prepared skin samples were treated with the active agent under study, inoculated with the microorganism in question and cultured. In this study, the activator was coated zinc pyrithione particles and the test organism used was Malassezia furfur. The relative efficacy of the treatment was determined by looking for growth inhibition compared to the control, and this data was collected using computer image analysis and vital staining. It shows that coated zinc pyrithione is more effective against Malassezia furfur than uncoated material. To test the efficacy of zinc pyrithione, both a 1% aqueous dispersion and 1% in a shampoo formulation were used. The formulated shampoo, whose test results are shown in Table 3 below, contained the following ingredients: STEPANOL AM-V anionic surfactant, 68.3%; STEPAN SAB-2 suspension NINOL 40-CO nonionic blowing agent, 2%; coated zinc pyrithione particles, 1% based on pyrithione content; GLYDANT preservative, 0.3%; 100% Residual deionized water for; all weight percentages are based on the total weight of the shampoo.

  Corneophanometry bioassays were performed on normal stratum corneum taken from 20 healthy volunteers using cyanoacrylate glue. Samples were soaked in olive oil and stored in a sterilized environment for 3 days. The corneal layer sample impregnated with olive oil was immersed in a shampoo containing 1% zinc pyrithione dispersion or 1% zinc pyrithione in the second stage. Further information from the “Corneophanometry” test can be found in “Clinical and Experimental Dermatology” (1989), Vol. 14, pp. 425-428. Rurangirwa, C.I. Peirard-Franchimont, and G.M. E. "Pulture of fungi on cyanoacrylate skin surface strippings-a quantitative bioassay for evaluating antifungal drugs" by Pierard C Available in technical journal papers.

Malassezia furfur was cultured in MLMA (Modified Leeming and Notman agar) media. A suspension of fungal cells was prepared in physiological saline (10 ⁴ to 10 ⁵ cells / ml). 250 μl portions of these suspensions were deposited on the corneal layer samples and incubated at 27 ° C. for 9 days in a wet state. Samples were stained using natural red as a vital stain to identify live fungi grown on the human corneal layer. The number of positive fungal cells was calculated in mm ² using computer image analysis (Analysis Olympus). The median and range were determined. A pair of non-parameter Friedman tests were used, followed by a product comparison using the Dunn test.

  As shown in the data shown in Tables 2 and 3, the sample coated with 11.7% palm oil (Example 1) demonstrated that it gave the best antifungal efficacy results (%). For comparison, 4.0% stearyl dimethicone (Comparative Example A) showed moderate efficacy over both the aqueous dispersion and formulated shampoo over the uncoated control, compared with 7.5% A sample coated with aminosilicone (Comparative Example B) showed some good improvement in efficacy with the aqueous dispersion, but only a slight improvement with the formulated shampoo. Surprisingly, the sample coated with lecithin (Comparative Example C) and siliconyl beeswax (Comparative Example D) shows a negative efficacy improvement in the aqueous suspension.

Although the invention has been described above with reference to specific embodiments thereof, it is apparent that many changes, modifications, and changes can be made without departing from the inventive concepts described herein. Accordingly, it is intended to embrace all such changes, modifications and variations that fall within the essence and broad scope of the appended claims.

Claims (27)

  A composition comprising coated particles partially or fully coated with a lipophilic agent of a pyrithione salt selected from the group consisting of monovalent and polyvalent pyrithione salts, and combinations thereof.   The composition of claim 1, wherein the pyrithione salt is selected from the group consisting of zinc, copper, silver, and zirconium salts, and combinations thereof.   The composition of claim 1, wherein the lipophilic agent comprises natural or synthetic fatty acids, fatty acid esters, or combinations thereof having a number average molecular weight in the range of about 150 to about 1,500.   The natural or synthetic fatty acid ester comprises a glyceride selected from the group consisting of mono-, di-, and tri-glycerides, and combinations thereof, having 8 to 22 carbon atoms in the backbone. A composition according to 1.   The composition of claim 1, wherein the pyrithione salt is present in the coated particles in a weight percent of about 10% to about 99%, based on the weight of the coated particles.   The composition of claim 1, wherein a lipophilic agent is present in the coated particles at a weight percent of about 0.01% to about 90%, based on the weight of the coated particles.   The composition according to claim 1, wherein the coated particles are individually and completely coated with a lipophilic agent.   The composition according to claim 1, wherein the coated particles comprise a coating having a thickness of 10 to 100 mm.   The lipophilic agent is selected from the group consisting of lanolin oil, fish oil, animal oil, coconut oil, palm oil, butter fat, soybean oil, sunflower oil, cottonseed oil, corn oil, and combinations thereof. Composition.   The composition according to claim 9, wherein the lipophilic agent is palm oil.   The composition according to claim 1, further comprising water or an organic solvent, wherein the lipophilic agent is insoluble in the organic solvent.   The composition according to claim 11, wherein the lipophilic agent is palm oil.   The composition according to claim 1, further comprising water or an organic solvent, wherein the lipophilic agent is soluble in the organic solvent.   14. The composition of claim 13, wherein the organic solvent is selected from the group consisting of methanol, ethanol, acetone, ether, halogenated hydrocarbons, and aromatic solvents.   A human care composition comprising a topical carrier and coated particles of a pyrithione salt selected from the group consisting of mono- and polyvalent pyrithione salts, and combinations thereof, partially or fully coated with a lipophilic agent .   The human care composition of claim 15, wherein the coated particles have a median particle size of about 0.1 to about 50 μm.   16. The human care composition of claim 15, wherein the metal salt of pyrithione is present in the composition in a form selected from the group consisting of blocks, platelets, spheres, bars, needles, and combinations thereof.   16. A human care composition according to claim 15, wherein the topical carrier is a shampoo comprising water, an organic solvent, or a combination thereof.   19. The human care composition of claim 18, wherein the organic solvent is selected from the group consisting of ethanol, propanol, isopropanol, glycerin, and combinations thereof. In a method of producing an aqueous dispersion of solid zinc pyrithione particles having solid palm oil coating on its outer surface with solid zinc pyrithione particles.
(A) heating an aqueous dispersion of solid zinc pyrithione particles to an elevated temperature of about 50 ° C. to about 80 ° C. to provide a high temperature aqueous dispersion;
(B) heating solid palm oil to an elevated temperature of about 50 ° C. to about 80 ° C. to provide liquid palm oil; and (c) mixing the liquid palm oil with the high temperature aqueous dispersion. Providing a mixture and cooling the mixture to provide an aqueous dispersion of solid zinc pyrithione particles having the solid palm oil coating;
The said manufacturing method containing.   18. Solid zinc pyrithione particles having a solid palm oil coating made according to the method of claim 17, wherein the coated particles of zinc pyrithione are present in an amount of about 40% to about 50% based on the total weight of the dispersion. Aqueous dispersion.   An aqueous antimicrobial dispersion concentrate comprising from about 25 wt% to about 50 wt% solid particles of pyrithione and water, based on the weight of the dispersion, wherein the solid particles of the pyrithione salt are lipophilic on the outer surface. Coated particles having a coating, wherein the coated particles have at least 25% antimicrobial activity in the bioassay test compared to the antimicrobial efficacy of the dispersion of uncoated particles of the pyrithione salt in a comparative Corneofundimetric bioassay test. The above aqueous antibacterial dispersion concentrate showing improved efficacy.   20. A human care composition comprising from about 0.5% to about 5% of the concentrate according to claim 19 based on the total weight of the topical carrier and the human care composition, wherein an equivalent amount of uncoated Comparison of human care compositions containing pyrithione salt particle dispersions The above human care compositions exhibit at least 15% improvement in antibacterial efficacy in the bioassay test compared to the antibacterial efficacy in the corneophagemetric bioassay test object.   An aqueous dispersion of solid zinc pyrithione particles having an average particle size of about 0.1 μ to about 5 μ, wherein the particles are coated with a coating of solid palm oil, the coating being 3 based on the total weight of the coated particles. The aqueous solid zinc pyrithione particle dispersion as described above, comprising from 15% to 15% by weight, wherein the coated particles are present in an amount of from about 40% to about 50% by weight, based on the total weight of the dispersion.   A composition comprising coated zinc pyrithione particles and water or an organic solvent, wherein the coated zinc pyrithione particles are coated with palm oil. In a method of coating pyrithione particles with a coating material,
(A) contacting the pyrithione particles with a solution of a coating material dissolved in an organic solvent;
(B) mixing the pyrithione particles with the coating material in the organic solvent, and (c) evaporating the organic solvent to give pyrithione particles coated with the coating material;
A pyrithione particle coating method comprising: 27. The method of claim 26, wherein the coating material is palm oil.