JP2007503802A5 - - Google Patents
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- JP2007503802A5 JP2007503802A5 JP2006524224A JP2006524224A JP2007503802A5 JP 2007503802 A5 JP2007503802 A5 JP 2007503802A5 JP 2006524224 A JP2006524224 A JP 2006524224A JP 2006524224 A JP2006524224 A JP 2006524224A JP 2007503802 A5 JP2007503802 A5 JP 2007503802A5
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- biomass
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- 239000001963 growth media Substances 0.000 claims 5
- 239000002028 Biomass Substances 0.000 claims 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 4
- 244000005700 microbiome Species 0.000 claims 4
- 238000000034 method Methods 0.000 claims 3
- 238000010923 batch production Methods 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- 241000195493 Cryptophyta Species 0.000 claims 1
- SBJKKFFYIZUCET-JLAZNSOCSA-N Dehydro-L-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-JLAZNSOCSA-N 0.000 claims 1
- 235000019749 Dry matter Nutrition 0.000 claims 1
- 241000233671 Schizochytrium Species 0.000 claims 1
- 241001467333 Thraustochytriaceae Species 0.000 claims 1
- 241000233675 Thraustochytrium Species 0.000 claims 1
- 229940029983 VITAMINS Drugs 0.000 claims 1
- 229940021016 Vitamin IV solution additives Drugs 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000010924 continuous production Methods 0.000 claims 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 claims 1
- 238000005755 formation reaction Methods 0.000 claims 1
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000011707 mineral Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229930003231 vitamins Natural products 0.000 claims 1
Claims (16)
- Y g/Lの細胞乾燥物質（CDM）（ここで、Yは、100〜300g/Lの範囲で存在する）で、好気性条件下で発酵槽において栄養要求性海洋微生物を連続して培養するための方法であって、培養液１L当たり（Y×h）g（hは、1.1〜3.0の範囲である）の量で徐々に添加され、そして20〜100時間の滞留時間をもって、炭素源を含んで成る培養培地において、前記栄養要求性海洋微生物を培養することを含んで成り、こので前記炭素源がバイオマスの生成のために制限的であり、当該生成されるバイオマスの少なくとも40％が、クロロホルム：メタノールの混合物により抽出できる成分から構成される、前記の方法。
- 前記培養培地が、培養液１L当たり（Y×h×f）（ここで、fは、0.002〜0.2の範囲である）の量で徐々に添加される、窒素源を含んで成る請求項１記載の方法。
- 前記培養培地が、達成されるバイオマス濃度のために制限的でない量で、徐々に添加される、バイオマス形成のために必要とされる塩、鉱物及び任意には、ビタミンを含んで成る請求項１記載の方法。
- ｈが、1.1〜2.5の範囲、特に1.2〜2.0の範囲である請求項１記載の方法。
- ｆが、0.004〜0.1の範囲、特に0.01〜0.04の範囲である請求項２〜４のいずれか１項記載の方法。
- 前記栄養要求性海洋微生物が、藻類である請求項１〜５のいずれか１項記載の方法。
- 前記栄養要求性海洋微生物が、トラウストキトリドsp.(Thraustochytrids sp.)である請求項１〜６のいずれか１項記載の方法。
- 前記トラウストキトリドsp.が、シゾキトリウム（Schizochytrium）又はトラウストキトリウム（Thraustochytrium）から成る群から選択される請求項１〜７のいずれか１項記載の方法。
- 前記培養温度が、20〜35℃の範囲である請求項１〜８のいずれか１項記載の方法。
- 前記培養培地のpHが、3.0〜9.0の範囲である請求項１〜９のいずれか１項記載の方法。
- 前記クロロホルム及びメタノールが、２：１（v/v）の比で混合される請求項１記載の方法。
- 少なくとも0.2gのDHA/l/hのポリエン酸生産性が達成される請求項１〜11のいずれか１項記載の方法。
- 前記連続培養工程における培養液の滞留時間が、一定に維持され、そして20〜100hの範囲である請求項１〜12のいずれか１項記載の方法。
- 前記連続培養工程における培養液の滞留時間が、20〜100の範囲内で変化する請求項１〜13のいずれか１項記載の方法。
- 前記連続的培養が、次の３培養段階：
ａ）初期バッチ工程、続いて
ｂ）フェドバッチ工程、続いて
ｃ）連続工程、
を含んで成る請求項１記載の方法。 - 前記溶解される酸素のレベルが、段階ｃ）の開始から10％以下の飽和で維持される請求項15記載の方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200301237 | 2003-09-01 | ||
| PCT/DK2004/000561 WO2005021735A1 (en) | 2003-09-01 | 2004-08-24 | Method for increasing yield of biomass of and/or components of biomass from marine microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007503802A JP2007503802A (ja) | 2007-03-01 |
| JP2007503802A5 true JP2007503802A5 (ja) | 2007-10-04 |
Family
ID=34259072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006524224A Pending JP2007503802A (ja) | 2003-09-01 | 2004-08-24 | 海洋微生物のバイオマス及び／又はそのバイオマスの成分の収量を高める方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20070015263A1 (ja) |
| EP (1) | EP1660639A1 (ja) |
| JP (1) | JP2007503802A (ja) |
| CN (1) | CN1845986A (ja) |
| RU (1) | RU2346033C2 (ja) |
| WO (1) | WO2005021735A1 (ja) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8207363B2 (en) | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| KR20150013667A (ko) | 2010-01-19 | 2015-02-05 | 디에스엠 아이피 어셋츠 비.브이. | 에이코사펜타엔산 생산 미생물, 지방산 조성물 및 이의 제조방법 및 용도 |
| US8889400B2 (en) | 2010-05-20 | 2014-11-18 | Pond Biofuels Inc. | Diluting exhaust gas being supplied to bioreactor |
| US8940520B2 (en) | 2010-05-20 | 2015-01-27 | Pond Biofuels Inc. | Process for growing biomass by modulating inputs to reaction zone based on changes to exhaust supply |
| US20120156669A1 (en) | 2010-05-20 | 2012-06-21 | Pond Biofuels Inc. | Biomass Production |
| US11512278B2 (en) | 2010-05-20 | 2022-11-29 | Pond Technologies Inc. | Biomass production |
| US8969067B2 (en) | 2010-05-20 | 2015-03-03 | Pond Biofuels Inc. | Process for growing biomass by modulating supply of gas to reaction zone |
| US20120276633A1 (en) | 2011-04-27 | 2012-11-01 | Pond Biofuels Inc. | Supplying treated exhaust gases for effecting growth of phototrophic biomass |
| CA2842272C (en) | 2011-07-21 | 2020-04-28 | Dsm Ip Assets B.V. | Fatty acid compositions |
| US9534261B2 (en) | 2012-10-24 | 2017-01-03 | Pond Biofuels Inc. | Recovering off-gas from photobioreactor |
| EP2826384A1 (de) | 2013-07-16 | 2015-01-21 | Evonik Industries AG | Verfahren zur Trocknung von Biomasse |
| JP6563393B2 (ja) | 2013-08-01 | 2019-08-21 | フェルマンタル | 珪藻バイオマスの生産方法 |
| FR3015516B1 (fr) * | 2013-12-19 | 2016-01-22 | Roquette Freres | Procede d'enrichissement en dha de la biomasse de microalgues du genre thraustochytrium |
| CN106795539B (zh) * | 2014-05-22 | 2021-06-22 | 玛拉可再生能源公司 | 在微生物中产生油的方法 |
| EP3200604B1 (de) | 2014-10-02 | 2021-11-03 | Evonik Operations GmbH | Verfahren zur herstellung eines futtermittels |
| EP3200603A1 (de) | 2014-10-02 | 2017-08-09 | Evonik Degussa GmbH | Pufas enthaltendes futtermittel mit hoher abriebfestigkeit und hoher wasserstabilität |
| US10619175B2 (en) | 2014-10-02 | 2020-04-14 | Evonik Operations Gmbh | Process for producing a PUFA-containing feedstuff by extruding a PUFA-containing biomass |
| CA2958457C (en) | 2014-10-02 | 2022-10-25 | Evonik Industries Ag | Process for producing a pufa-containing biomass which has high cell stability |
| JP6773301B2 (ja) * | 2014-10-16 | 2020-10-21 | マラ リニューアブルズ コーポレーション | 反復フェドバッチ培養方法 |
| CA3094477A1 (en) * | 2018-03-30 | 2019-10-03 | Dsm Ip Assets B.V. | Method of obtaining a microbial oil and a method of reducing emulsion by maintaining a low concentration of carbohydrate |
| KR102100650B1 (ko) * | 2018-06-29 | 2020-04-16 | 씨제이제일제당 주식회사 | 신규한 트라우즈토카이트리움 속 균주, 및 이를 이용한 다중불포화지방산 생산방법 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340742A (en) * | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| US5407957A (en) * | 1990-02-13 | 1995-04-18 | Martek Corporation | Production of docosahexaenoic acid by dinoflagellates |
| US5244921A (en) * | 1990-03-21 | 1993-09-14 | Martek Corporation | Eicosapentaenoic acids and methods for their production |
| CA2121986A1 (en) * | 1993-04-26 | 1994-10-27 | Daizo Takeuchi | Processes for culturing marine microalgae and producing docosahexaenoic acid using the same |
| EP0823475B1 (en) * | 1995-04-17 | 2009-06-17 | National Institute of Advanced Industrial Science and Technology | Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms |
| WO2000001686A1 (en) * | 1998-07-06 | 2000-01-13 | Eastman Chemical Company | Method of producing vitamin e |
| EP2341127B1 (en) * | 2000-01-28 | 2015-05-27 | DSM IP Assets B.V. | Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors |
-
2004
- 2004-08-24 EP EP04739046A patent/EP1660639A1/en not_active Withdrawn
- 2004-08-24 US US10/570,398 patent/US20070015263A1/en not_active Abandoned
- 2004-08-24 WO PCT/DK2004/000561 patent/WO2005021735A1/en active Application Filing
- 2004-08-24 CN CNA200480025011XA patent/CN1845986A/zh active Pending
- 2004-08-24 JP JP2006524224A patent/JP2007503802A/ja active Pending
- 2004-08-24 RU RU2006110569/13A patent/RU2346033C2/ru not_active IP Right Cessation
-
2009
- 2009-06-29 US US12/493,505 patent/US20090263889A1/en not_active Abandoned
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