JP2007269667A - Antibacterial agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antibacterial agent by using a natural material without having problems in safety such as skin irritation, etc., and a cosmetic and skin preparation for external use blended with the antibacterial agent. <P>SOLUTION: The cosmetic and skin preparation for external use having a sufficient antibacterial activity and excellent in safety are provided by containing an extract obtained from Camellia sasanqua especially the extract obtained from the leaves of the Camellia sasanqua, by ≥0.1%, without using a preservative having skin irritation such as para-oxybenzoic acid ester, etc. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

The present invention relates to an antibacterial agent containing a Zazanka leaf extract which is a natural material and has excellent antibacterial properties.

In cosmetics, antibacterial agents are used for the purpose of suppressing the growth of microorganisms mixed in from the outside and killing them with time to prevent the deterioration of the product. As a representative one, paraoxybenzoic acid esters are widely used. Although paraoxybenzoic acid esters are widely used in foods, there is a history of allergies in the past, and problems with skin irritation have been pointed out. Yes. In addition, benzoate, salicylate, and dehydroacetate are often used, but skin irritation problems have been pointed out as in the case of paraoxybenzoate.

In recent years, there has been a movement to provide antibacterial properties with phenoxyethanol, but the problem of skin irritation cannot be avoided. As natural antibacterial substances, hinokitiol, monocaprylic acid glycerides, cationic amino acid derivatives, bisabolol, glycols, rosemary extract as a plant extract, grapefruit seed extract, etc. are known. It has also been tried to suppress the growth of microorganisms by using them together. However, hinokitiol is known to brown in the presence of iron ions. In glyceryl monocaprylate, caprylic acid in which an ester bond is liberated by hydrolysis action in the presence of water is generated, and its odor is not preferable.
JP 2002-226386 A JP 2003-1113013 A

An object of the present invention is to provide a new antibacterial agent having no safety problems such as skin irritation.

As a result of intensive studies in view of the circumstances, the present inventor has found that the extract of the leaves of sasanqua has antibacterial activity, and has completed the present invention. That is, the present invention is an antibacterial agent characterized by an extract of sasanqua leaves.

According to the present invention, by adding an antibacterial agent characterized by containing a sasanqua leaf extract, a safety having a sufficient antibacterial action without using a preservative having skin irritation such as paraoxybenzoate ester It is possible to provide cosmetics with excellent properties.

BEST MODE FOR CARRYING OUT THE INVENTION

The extract of the sasanqua leaves of the present invention exhibits sufficient antibacterial properties by itself, but a small amount of ethanol, phenoxyethanol, hinokitiol, monocaprylic acid glycerides, cationic amino acid derivatives, bisabolol and 1,2-pentanediol, 1, By using an antibacterial agent such as 2-hexanediol in combination, the antibacterial action and the antibacterial range are synergistically improved.

In addition to the above-mentioned essential components, the antibacterial agent of the present invention includes other components used in normal cosmetics, pharmaceuticals, quasi drugs, etc., for example, vitamin B2, vitamin B6, vitamin C, vitamin D Vitamins such as nicotinic acids, vitamin E, vitamin P, biotin, amino acids and amino acid derivatives, oils such as jojoba oil and squalane, glycols such as glycerin, sorbitol, and 1,3-butylene glycol, collagen, hyaluronic acid, etc. Polymer moisturizers, antioxidants, surfactants, flame retardants such as glycyrrhizic acid derivatives, various plant extracts, thickeners such as carboxyvinyl polymer, flavoring, water, alcohol, colorants, resin powders such as polyethylene Etc. can be appropriately blended as necessary.

The blending amount of the extract of the leaf of sasanqua that can be used in the present invention with respect to various cosmetics can be varied according to the embodiment of the cosmetics, the usage form of the cosmetics, etc., and is not particularly limited. In principle, an effective amount may be present in the cosmetic, but the general blending amount is 0.001 to 5% by mass, preferably 0.01 to 3.0% by mass in the cosmetic composition. More preferably, it is 0.1 to 1.0% by mass, but this is an indication that the appropriate amount cannot be particularly limited depending on the dosage form of the cosmetic and the content of the formulation.

The range of application to the cosmetic according to the present invention is not limited by the dosage form, and can be applied to all cosmetics.

For example, the active ingredients according to the present invention include various basic cosmetics such as creams, milky lotions, lotions, packs, facial cleansers, foundations, lipsticks, cheeks, white powders, makeup products, hair shampoos, hair nourishing agents, shampoos, rinses. It is applicable to other cosmetics such as various hair cosmetics such as soap, beauty nail, and cologne. The embodiments of the various cosmetics can also be applied to various embodiments such as solutions, emulsions, ointments, sols, gels, powders, and sprays.

EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, this invention is not limited to an Example.

[Manufacture of sasanqua leaf extract]
First, the manufacture example of the extract of the leaf of a sasanqua characteristic in the skin external preparation concerning this invention is demonstrated.

[Preparation of extract obtained from Example 1]
As raw material, 20 g of sasanqua leaves were used. 100 mL of ethanol is added to 20 g of the raw material, and the mixture is heated and extracted at 90 ° C. for 6 hours. The supernatant is centrifuged to remove relatively large suspended matters, and then filtered using a filter paper having a mesh diameter of 0.22 μm. Concentration under reduced pressure using a rotary evaporator gave about 0.5 g of sasanqua leaf extract.

[Antimicrobial activity against bacteria]
E. The antibacterial action of the plant extract was examined by the paper disk method (using a paper disk having a diameter of 6 mm) using E. coli. The sample was used by dissolving the above extract in dimethyl sulfoxide (DMSO) so as to be 1 w / v%. Culture is Soybean-Casein Digest
Broth (SCDLP) medium was used under anaerobic conditions at 37 ° C. for 3 days. The results are shown in Table 1. As can be seen from Table 1, the antibacterial and bactericidal action was confirmed in the extract obtained from the sasanqua extract at a concentration of 1%. The antibacterial action was evaluated according to the following criteria.
++: Blocking circle diameter 20 mm or more +; Blocking circle diameter 12 mm or more ±; Blocking circle diameter 8 mm or less

[Antimicrobial activity against fungi]
A. Using niger, the antibacterial action of the plant extract was examined by the paper disc method (using a paper disc having a diameter of 6 mm). The sample was used by dissolving the above extract in dimethyl sulfoxide (DMSO) so as to be 1 w / v%. Culture is Glucose Peptone Agar
With Lecithin & Polysorbate 80 (GDLP) medium, it was performed under anaerobic conditions at 37 ° C. for 3 days. The results are shown in Table 2. As can be seen from Table 2, antibacterial and bactericidal action was confirmed in the extract obtained from the sasanqua extract at a concentration of 1%. The antibacterial action was evaluated according to the following criteria.
++: Blocking circle diameter 20 mm or more +; Blocking circle diameter 12 mm or more ±; Blocking circle diameter 8 mm or less

[Experiment 2: Confirmation of antibacterial activity (challenge test)]
The extract of sasanqua leaves was subjected to antibacterial confirmation tests against five legal species (S. aureus, E. coli, Ps. Aerginosa, C. arbicans, A. niger). As samples, a lotion containing a sasanqua leaf extract and a lotion containing ethanol as a solvent for the sasanqua leaf extract were prepared and subjected to the test.

[Criteria]
Evaluation criteria were evaluated in the following five stages.
(Double-circle): The remarkable reduction | decrease in the number of bacteria was seen.
○: A decrease in the number of bacteria was observed.
Δ: A moderate decrease in the number of bacteria was observed.
▲: Decrease or increase in the number of bacteria was not observed ×: Increase in the number of bacteria was observed.

Table 3 shows the effect on “fungi” for the antibacterial action including ethanol as a solvent for the sasanqua leaf extract.

Table 4 shows the effect on “bacteria”.

From the results of Tables 3 and 4, it was confirmed that sufficient antibacterial action was exhibited to maintain the quality of cosmetics without using paraoxybenzoate.

Next, the specific cosmetics using the sasanqua leaf extract and ethanol according to the present invention will be described.

The formulation example of this invention is given to the following.
<Prescription Example 1> Lotion (mass%)
Sasanqua leaf extract 5.0
Ethanol 5.0
Glycerin 5.0
Polyoxyethylene sorbitan monolaurate (20E.0) 1.5
Antioxidant Appropriate amount of purified water balance

<Formulation example 2> Cosmetic cream (mass%)
Sasanqua leaf extract 0.001
Ethanol 5.0
Beeswax 2.0
Stearyl alcohol 5.0
Stearic acid 8.0
Squalane 10.0
Self-emulsifying glyceryl monostearate 3.0
Polyoxyethylene cetyl ether (20E.0) 1.0
1,3-butylene glycol 5.0
Potassium hydroxide 0.3
Perfume Appropriate amount Antioxidant Appropriate amount Purified water The rest

<Prescription Example 3> Emulsion (mass%)
Sasanqua leaf extract 1.0
Ethanol 5.0
Squalane 8.0
Vaseline 2.0
Beeslow 0.5
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20E.0) 1.2
Carboxyvinyl polymer 0.2
Potassium hydroxide 0.1
1.3-Butylene glycol 7.0
Perfume Appropriate amount Antioxidant Appropriate amount Purified water The rest

<Prescription Example 4> Packing agent (mass%)
Sasanqua leaf extract 0.1
Ethanol 5.0
Vinyl acetate resin emulsion 15.0
Polyvinyl alcohol 10.0
Jojoba oil 3.0
Glycerin 5.0
Titanium oxide 8.0
Kaolin 7.0
Perfume Appropriate amount Antioxidant Appropriate amount Purified water The rest

<Prescription Example 5> Ointment (mass%)
Sasanqua leaf extract 3.0
Ethanol 5.0
Tocopherol acetate 0.5
Octyl paradimethylaminobenzoate 4.0
Butylmethoxybenzoylmethane 4.0
Stearyl alcohol 18.0
Owl 20.0
Glycerin monostearate 0.3
Vaseline 33.0
Perfume Appropriate amount Antioxidant Appropriate amount Purified water The rest

<Prescription Example 6> Eyeshadow (mass%)
Sasanqua leaf extract 0.01
Ethanol 5.0
Antioxidant appropriate amount liquid paraffin 6.0
Methylpolysiloxane 2.0
Perfume appropriate amount sorbitan sesquioleate 2.0
Talc 45.0
Mica 15.0
Sericite 5.0
Pigment 14.5
Pearl pigment 10.0

The cosmetics prepared in the above-mentioned prescription examples 1 to 6 were used by 20 healthy female panelists (120 persons in total) for each cosmetic for 2 weeks, and the use feeling and skin irritation were confirmed. As a result, no skin irritation was seen by one person, and an answer that fully satisfied the feeling of use was obtained.

According to the present invention, there is provided a cosmetic comprising an antibacterial agent characterized by containing an extract of sasanqua leaves, and the cosmetic has sufficient antibacterial activity without using a paraoxybenzoic acid ester. By having, it is excellent in safety.

Claims (3)

An antibacterial agent characterized by containing an extract obtained from Sasanqua. The antibacterial agent according to claim 1, wherein the extract of sasanqua is obtained from leaves of sasanqua. A topical skin preparation characterized by containing 0.1% by weight or more of sasanqua extract as an antibacterial composition.