JP2007259913A - Apparatus and method for measuring component concentration - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To precisely calibrate measurement of component concentration by reducing affection of the change with time of a body to be examined or a matter to be measured which is to be irradiated with light and also that of a sonic wave detector. <P>SOLUTION: This apparatus is provided with: a light outgoing means which electrically modulates intensity of lights of two waves having the same wavelength or mutually different wavelengths respectively by signals of the same frequency and opposite phases and makes the light outgo; a standard sample which is irradiated with one of the lights of the two waves from the light outgoing means; and a sonic wave detection means which detects a sonic wave generated from the standard sample by the one light from the light outgoing means and a sonic wave generated by the other light from the body to be examined or the matter to be measured irradiated with the other light from the light outgoing means at the same position with respect to the standard sample and the body to be examined or the matter to be measured irradiated with the light. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a non-invasive component concentration measuring apparatus and component concentration measuring method for a human or animal subject, or a component concentration measuring apparatus and component concentration measuring method for an object collected from a human or animal.

  With the aging of society, dealing with adult diseases is becoming a major issue. In blood glucose level and other tests, blood collection is necessary, which places a heavy burden on the patient. Therefore, a non-invasive component concentration measurement apparatus that does not collect blood has attracted attention. As a non-invasive component concentration measuring device that has been developed so far, the skin is irradiated with electromagnetic waves and absorbed by blood molecules to be measured, for example, glucose molecules in the case of blood glucose levels, and heated locally. A photoacoustic method that observes a sound wave generated from a living body due to thermal expansion has attracted attention.

  However, the interaction between glucose and electromagnetic waves is small, and there is a limit to the intensity of electromagnetic waves that can be safely irradiated to a living body, so that a sufficient effect has not been achieved in measuring blood glucose levels in the living body.

  7 and 8 are diagrams showing a configuration example of a conventional blood component concentration measuring apparatus using a photoacoustic method as a conventional example. FIG. 7 shows a first conventional example in which a light pulse is used as an electromagnetic wave (see, for example, Non-Patent Document 1). In this example, blood glucose, that is, glucose is the measurement target as the blood component. In FIG. 7, a drive circuit 604 supplies a pulsed excitation current to a pulse light source 616, the pulse light source 616 generates a light pulse having a sub-microsecond duration, and the generated light pulse is applied to a subject 610. The The light pulse generates a sound wave called a pulsed photoacoustic signal inside the subject 610, and the generated sound wave is detected by the ultrasonic detector 613 and further converted into an electric signal proportional to the sound pressure.

  The waveform of the converted electric signal is observed by the waveform observer 620. This waveform observer 620 is triggered by a signal synchronized with the excitation current, the converted electrical signal is displayed at a fixed position on the tube surface of the waveform observer 620, and the converted electrical signal is measured by integrating and averaging. can do. The amplitude of the electrical signal thus obtained is analyzed, and the blood glucose level inside the subject 610, that is, the amount of glucose is measured. In the case of the example shown in FIG. 7, an optical pulse having a sub-microsecond pulse width is repeatedly generated at a maximum of 1 kHz, and 1024 optical pulses are averaged to measure the electrical signal. However, sufficient accuracy is obtained. It is not done.

  Therefore, a second conventional example using a light source that is continuously intensity-modulated has been disclosed for the purpose of improving accuracy. FIG. 8 shows a configuration of a device of the second conventional example (see, for example, Patent Document 1). In this example as well, blood sugar is the main measurement target, and high accuracy is attempted using a plurality of light sources having different wavelengths. In order to avoid complicated explanation, the operation when the number of light sources is 2 will be described with reference to FIG. In FIG. 8, light sources having different wavelengths, that is, a first light source 601 and a second light source 605 are driven by a drive circuit 604 and a drive circuit 608, respectively, and output continuous light.

  Light output from the first light source 601 and the second light source 605 is intermittently driven by a chopper plate 617 that is driven by a motor 618 and rotates at a constant rotational speed. Here, the chopper plate 617 is formed of an opaque material, and a relatively small number of openings are formed on the circumference around which the light of the first light source 601 and the second light source 605 passes with the axis of the motor 618 as the center. Is formed.

With the above-described configuration, the light output from each of the first light source 601 and the second light source 605 is intensity-modulated with the disjoint modulation frequency f ₁ and modulation frequency f ₂ and then combined by the combining unit 609. The object 610 is irradiated as one light beam.

The inside of the subject 610 photoacoustic signal having the frequency f ₁ is generated by the light of the first light source 601, the photoacoustic signal having the frequency f ₂ is generated by the light of the second light source 605, these photoacoustic signal Is detected by the acoustic sensor 619 and converted into an electric signal proportional to the sound pressure, and its frequency spectrum is observed by the frequency analyzer 621. In this example, the wavelengths of the plurality of light sources are all set to the absorption wavelength of glucose, and the intensity of the photoacoustic signal corresponding to each wavelength is measured as an electrical signal corresponding to the amount of glucose contained in the blood. The

Here, the relationship between the intensity of the measured value of the photoacoustic signal and the value obtained by measuring the glucose content by separately collected blood is stored in advance, and the amount of glucose is measured from the measured value of the photoacoustic signal. ing.
JP-A-10-189 University of Oulu (University of Oulu, Finland) thesis “Pulse photoacoustic technique and glucodesis in human blood and tissue” (IBS 951-42-6690-0, ul./200.

  The conventional example described above has the following problems.

  In other words, since the measurement target of the component concentration is a living body or liquid, the measurement state always changes during the measurement or every measurement day depending on the measurement environment such as the measurement temperature and the atmospheric pressure when measuring the component concentration and the physical condition of the living body itself. . Also, the performance of the parts of the measuring device changes with time. This is the same in the above-described conventional example. Therefore, in order to reduce the influence of interfering factors on the component concentration measurement included in the measurement target and the change over time of the performance of the device parts, it is usually considered to calibrate the values measured during or after the measurement.

  However, when different elements are used as acoustic detectors for detecting sound waves during calibration, an operation error occurs in the detected sound wave values due to individual differences in the element characteristics of the acoustic detectors. On the other hand, when the same element is used, an error occurs in the detected sound wave value because the measurement state changes due to time-dependent fluctuation of measurement conditions such as removal / attachment of the measurement object, temperature, and atmospheric pressure.

  Therefore, in the above-described conventional example, there is a problem in that the calibration accuracy deteriorates.

  In order to solve the above problems, the present invention irradiates light toward a subject or an object to be measured, and also irradiates light to a standard sample necessary for calibration of component concentration, and generates sound waves generated from both. A component concentration measuring device and a component concentration measuring method that enable accurate calibration by making the detection time and detection state of sound waves the same by simultaneously detecting with one sound wave detector.

  First, the basic principle of the component concentration measuring apparatus of the present invention will be described as an example in the case of measuring the component concentration of a subject.

  In the present invention, the wavelength of the first light among the two different wavelengths of light is set to a wavelength that is significantly different from the absorbance due to water, for example, where the absorbance due to the measurement target component of the subject occupies most of the subject. The wavelength of the second light is set to a wavelength that exhibits an absorbance equal to that of water at the wavelength of the first light. The above-described wavelength setting method will be described with reference to FIG. 1, taking as an example the case of measuring the concentration of glucose in blood.

FIG. 1 shows the absorbance characteristics of water and an aqueous glucose solution at room temperature. In FIG. 1, the vertical axis indicates the absorbance, and the horizontal axis indicates the wavelength of light. In FIG. 1, the solid line indicates the absorbance characteristic of water, and the broken line indicates the absorbance characteristic of the glucose aqueous solution. The wavelength λ ₁ shown in FIG. 1 is a wavelength at which the absorbance due to glucose is significantly different from the absorbance due to water, and the wavelength λ ₂ is a wavelength at which water has the same absorbance as that at λ ₁ . Thus, for example, the wavelength of the first light can be set to λ ₁ and the wavelength of the second light can be set to λ ₂ .

In the following description, as an example, the wavelength of the first light is set to a wavelength λ ₁ where the absorbance of the component to be measured is significantly different from the absorbance of water, and the wavelength of the second light is set to the first light. The case where the wavelength λ ₂ is set to be equal to that at the wavelength λ ₁ will be described.

  Each of the two different wavelengths of light set as described above is intensity-modulated with a signal of the opposite phase at the same frequency and emitted as pulsed light, and the emitted two different wavelengths of light are absorbed by the component of the subject. Then, the generated sound wave is detected, and the concentration of the component to be measured of the subject is measured from the magnitude of the detected sound wave. When light of two different wavelengths whose intensity is modulated as described above is emitted, the first sound wave generated from the subject by the absorption of the first light by both the component to be measured and water, and the second light The second sound wave generated from the subject by absorbing water occupying most of the subject has the same frequency and an opposite phase. Therefore, the first sound wave and the second sound wave are superimposed in the subject, and only the magnitude of the sound wave generated from the subject due to absorption of the component to be measured in the first sound wave is obtained as the difference between the sound waves. Remains. Therefore, only the sound wave generated from the subject by the first light being absorbed by the component to be measured can be measured by the remaining sound wave. In the above measurement, the measurement target component absorbs rather than the difference between the sound wave generated by the absorption of both the measurement target component and water and the sound wave generated by the water absorption. Thus, the sound wave generated from the subject can be accurately measured.

Furthermore, a method for measuring with high accuracy, excluding the cause of errors in the sound wave measurement system such as the contact state between the subject and the sound wave detection element, will be described below. For each of the light of wavelength λ _{1 and} the light of wavelength λ ₂ , the absorption coefficient of water occupying most of the subject is α ₁ ^(w) and α ₂ ^(w) , and the molar absorption of the component to be measured of the subject If the coefficients are α ₁ ^(g) and α ₂ ^(g) , the simultaneous equations including the magnitudes s ₁ and s ₂ of sound waves generated from the subject by the light of wavelength λ _{1 and} the light of wavelength λ ₂ are It is expressed by Equation (1).

By solving the above equation (1), the component concentration M of the subject to be measured can be obtained. Here, C is a coefficient that is difficult to control or predict, that is, the coupling state between the subject and the sound wave detection element, the sensitivity of the sound wave detection element, and the distance between the position where the sound wave is generated by light in the subject and the sound wave detection element. , specific heat and thermal expansion coefficient of the object, the speed of the internal wave of the subject, the wavelength lambda ₁ of light and the wavelength lambda ₂ of the light modulation frequencies, the molar absorption coefficient of the component of the absorption coefficient and the subject of water, etc. It depends on the unknown constant. Further, when C is eliminated by Expression (1), the following Expression (2) is obtained.

Here, the absorption coefficients α ₁ ^(w) and α ₂ ^{(w) of} water occupying most of the subject for each of the light of wavelength λ _{1 and} the light of wavelength λ ₂ are selected to be equal. , Α ₁ ^(w) = α ₂ ^(w) is satisfied, and the fact that s ₁ ≈s ₂ is used, the component concentration M is expressed by Equation (3).

Substituting α ₁ ^(w) , α ₁ ^(g), and α ₂ ^(g) as known coefficients into the above equation (3), and further by the light of wavelength λ _{1 and} the light of wavelength λ ₂ , respectively. The component concentration M of the subject can be calculated by measuring and substituting the magnitudes s ₁ and s ₂ of the sound waves generated from the subject. In the above mathematical formula (3), rather than measuring the _two sound wave sizes s ₁ and s ₂ individually, the difference s ₁ -s ₂ is measured and the sound wave size s ₂ measured separately is measured. The component concentration of the subject can be measured with high accuracy.

Therefore, in the component concentration measurement apparatus of the present invention, first, the light of wavelength λ _{1 and} the light of wavelength λ ₂ are intensity-modulated by modulation signals having opposite phases to each other, and are combined into one light beam and emitted. Thus, the difference (s ₁ −s ₂ ) between the sound waves generated by superimposing the sound wave magnitude s ₁ and the sound wave magnitude s ₂ generated from the subject is measured. Next, light of wavelength λ ₂ is emitted, and the magnitude s _{2 of the} sound wave generated from the subject is measured. By calculating (s ₁ −s ₂ ) ÷ s ₂ from (s ₁ −s ₂ ) and s ₂ measured as described above, the component concentration of the measurement target of the subject is increased by Equation (3). It can be measured with high accuracy.

  The influence of the change of the subject over time in the component concentration measuring apparatus and the component concentration measuring method of the present invention is as follows.

In the present invention, the value of (s ₁ -s ₂ ) of the molecule in Formula (3) is measured as the magnitude of a sound wave generated in a living body by electrically irradiating two different wavelengths of light and simultaneously irradiating them with light. Can do. On the other hand, the value of s _{2 in} the denominator can be measured as the magnitude of a sound wave generated in a living body by separately irradiating light of a predetermined one wavelength among two different wavelengths of light. For this reason, the time for emitting light of two different wavelengths is naturally different from the time for emitting light of a predetermined wavelength.

  That is, the state of the living body changes in two measurements.

  Therefore, in the present invention, light is irradiated toward the subject or the object to be measured, and the standard sample necessary for calibration of the component concentration is also irradiated, and sound waves generated from both are simultaneously emitted by one sound wave detector. It was decided to detect.

  The above is the basic principle of the component concentration measuring apparatus and component concentration measuring method of the present invention.

  Specifically, the component concentration measuring apparatus according to the present invention includes: a light emitting unit that emits two light beams having the same wavelength or different wavelengths from each other by electrically modulating the intensity with signals having the same frequency and opposite phases; A standard sample irradiated with one of the two light beams from the light emitting means, a sound wave generated from the standard sample by the one light from the light emitting means, and the other from the light emitting means A sound wave generated by the other light from the subject or object to be irradiated with the light is detected at the same position with respect to the standard sample and the object or the object to be irradiated with the light. Sound wave detecting means.

  In the present invention, since the sound wave detecting means detects the sound wave at the same position, the magnitude of the sound wave generated by the two substances, that is, the subject or the object to be measured and the standard sample irradiated with the light from the light emitting means. Can be detected simultaneously. Therefore, it is possible to correct changes with time of the components of the standard sample in the subject or object to be irradiated with light. It is also possible to correct the influence of the components of the standard sample other than the measurement target in the subject or object to be irradiated with light. Furthermore, since the sound wave detecting means detects the sound wave at the same position, there is no sound measurement error due to individual differences of the sound wave detecting means. Therefore, in the present invention, the component concentration of the analyte or the analyte can be accurately measured as compared with the case where the two substances are separately measured and corrected after recording.

  In the component concentration measurement apparatus of the present invention, the light emission is performed so that the magnitude of the sound wave generated from the water becomes zero in a state where the two light beams having different wavelengths are emitted from the light emission unit to the water, respectively. It is desirable to further have wavelength adjusting means for adjusting the wavelength of the two light beams in the means.

  In the present invention, the wavelength adjusting means adjusts the wavelength of the two light beams so that the size of the sound wave generated from the water becomes zero, thereby changing the wavelength of the two light lights emitted from the light emitting means to the absorbance of water. Two predetermined wavelengths having matching characteristics can be obtained. As a result, the influence of water absorbance can be removed in advance from the sound wave to be detected. Therefore, in the present invention, it is not necessary to correct the water absorbance component when calculating the component concentration of the subject to be measured or the object to be measured, and the measurement can be performed accurately.

  The component concentration measurement apparatus according to the present invention includes a measurement light modulation unit that electrically modulates intensity of two waves of the same wavelength with a signal having the same frequency and an opposite phase, and outputs the same wavelength of the measurement modulation unit. A reference light modulation means for electrically modulating and outputting two waves of light having a wavelength different from that of the two waves by a signal having the same frequency as that of the intensity modulation of the measurement light modulation means and an opposite phase signal; A combined light emitting means for combining two light beams of opposite phases out of the two light beams from the measurement light modulating means and the two light lights from the reference light modulating means, and emitting two lights; From the standard sample irradiated with one of the two lights from the combined light emitting means, the sound wave generated from the standard sample by the one light from the combined light emitting means, and the combined light emitting means Subject to be irradiated with the other light or measurement The waves generated by the other light from, and a sound wave detecting means for detecting at the same position relative to the subject or the object to be measured is irradiated the standard sample, and the light.

  In the present invention, since the sound wave detecting means detects the sound wave at the same position, the magnitude of the sound wave generated by the two substances, that is, the subject or the object to be measured and the standard sample irradiated with the light from the light emitting means. Can be detected simultaneously. Therefore, it is possible to correct changes with time of the components of the standard sample in the subject or object to be irradiated with light. It is also possible to correct the influence of the components of the standard sample other than the measurement target in the subject or object to be irradiated with light. Furthermore, since the sound wave detecting means detects the sound wave at the same position, there is no sound measurement error due to individual differences of the sound wave detecting means. Therefore, in the present invention, the component concentration of the analyte or the analyte can be accurately measured as compared with the case where the two substances are separately measured and corrected after recording.

  In the component concentration measuring apparatus of the present invention, the measurement light modulating means and the measurement light modulating means and the measuring light so that the magnitude of the sound wave generated from the water becomes zero when the two lights are emitted from the combined light emitting means to the water, respectively. It is desirable to further include wavelength adjusting means for adjusting the wavelength of the two light beams in the reference light modulating means.

  In the present invention, the measurement light modulation means and the reference light modulation means adjust the wavelength of the two light beams so that the magnitude of the sound wave generated from water becomes zero, whereby two waves emitted from the combined light emission means. The wavelength of the light can be set to two predetermined wavelengths in which the absorbance characteristics of water coincide with each other in advance. As a result, the influence of water absorbance can be removed in advance from the sound wave to be detected. Therefore, in the present invention, it is not necessary to correct the water absorbance component when calculating the component concentration of the subject to be measured or the object to be measured, and the measurement can be performed accurately.

  In the component concentration measuring apparatus of the present invention, it is desirable that the sound wave detecting means is disposed at a position approximately equidistant from the standard sample and the subject or the object to be measured irradiated with the light.

  In the present invention, since the sound waves are detected at substantially equidistant positions, the attenuation with respect to the propagation distance of the sound waves is substantially the same, so that a measurement error of the component concentration measurement accompanying the attenuation of the sound waves does not occur. Therefore, in the present invention, the component concentration of the subject or the object to be measured can be accurately measured.

  In the component concentration measuring apparatus of the present invention, an elliptical reflector in which two or more concave elliptical spherical surfaces capable of reflecting the sound wave are arranged so that either one of the two focal points of each elliptical spherical surface is common is further provided. The standard sample is disposed at a position corresponding to another focal point excluding the common focal point of the ellipsoidal sphere, and the sound wave detecting means is configured to detect the standard sample and the common focal point and the other focal point of the ellipsoidal sphere. Corresponds to the common focus of the elliptical sphere so as to detect sound waves reflected by the elliptical sphere among the acoustic waves generated from the subject or the object to be measured that are arranged at other focal points of the elliptical sphere. It is desirable to be arranged in a position.

  In the present invention, due to the nature of an ellipse, a sound wave generated at the focal point of the ellipse can be collected at another focal point. For this reason, the sound wave detection means can detect sound waves with high sensitivity by reducing detection omission of sound waves. Therefore, in the present invention, the component concentration of the subject or the object to be measured can be accurately measured.

  In the component concentration measuring apparatus of the present invention, the light is filled with the standard sample, the object or the object to be irradiated with light, and the space between the acoustic wave detecting means and the elliptical spherical surface of the elliptical reflector. It is desirable to further include an acoustic matching material having an acoustic impedance substantially equal to the acoustic impedance of the subject or the object to be measured.

  In the present invention, the acoustic matching substance can improve the transmission efficiency of sound waves generated from the standard sample and the subject or the object to be measured. For this reason, attenuation due to the propagation distance of the sound wave can be reduced, and the sound wave detecting means can detect the sound wave with high sensitivity. Therefore, in the present invention, the component concentration of the subject or the object to be measured can be accurately measured.

  In the component concentration measuring apparatus of the present invention, it is desirable that the standard sample is water, blood, serum, or a bioequivalent substance.

  In the present invention, the component concentration measurement value can be corrected with water, blood, serum, or a bioequivalent substance for the standard sample according to the component to be measured of the subject or the object to be measured. Therefore, in the present invention, the component concentration of the subject or the object to be measured can be accurately measured.

  In the component concentration measuring method according to the present invention, the light emitting means emits two light beams having the same wavelength or different wavelengths from each other by electrically modulating the intensity with signals having the same frequency and opposite phases, and the sound wave detecting means A sound wave generated by the one light from a standard sample irradiated with one of the two waves of light from the light emitting means and the other light of the two waves of light from the combined light emitting means are irradiated. First sound wave detection that detects a sound wave generated by the other light from the subject or the object to be measured at the same position with respect to the standard sample and the object or the object to be measured irradiated with the light Provide procedures.

  In the present invention, since the sound wave detecting means detects the sound wave at the same position, the magnitude of the sound wave generated by the two substances, that is, the subject or the object to be measured and the standard sample irradiated with the light from the light emitting means. Can be detected simultaneously. Therefore, it is possible to correct changes with time of the components of the standard sample in the subject or object to be irradiated with light. It is also possible to correct the influence of the components of the standard sample other than the measurement target in the subject or object to be irradiated with light. Furthermore, since the sound wave detecting means detects the sound wave at the same position, there is no sound measurement error due to individual differences of the sound wave detecting means. Therefore, in the present invention, the component concentration of the analyte or the analyte can be accurately measured as compared with the case where the two substances are separately measured and corrected after recording.

  In the component concentration measurement method of the present invention, the light emitting means emits light of two waves having different wavelengths from each other by electrically modulating the intensity with signals of the same frequency and opposite phase, respectively. A wavelength adjusting procedure for adjusting the wavelength of the two light beams so that the wavelength adjusting means for adjusting the wavelength of the two light beams adjusts the wavelength of the two light beams so that the size of the sound wave generated from the water becomes zero. It is desirable to provide further.

  In the present invention, the wavelength adjusting unit includes a wavelength adjusting procedure for adjusting the wavelength of the two light beams so that the size of the sound wave generated from the water becomes zero. The wavelength can be set to two predetermined wavelengths that match the absorbance characteristics of water. As a result, the influence of water absorbance can be removed in advance from the sound wave to be detected. Therefore, in the present invention, it is not necessary to correct the water absorbance component when calculating the component concentration of the subject to be measured or the object to be measured, and the measurement can be performed accurately.

  In the component concentration measurement method of the present invention, after the first sound wave detection procedure, the same standard sample as the standard sample in the first sound wave detection procedure, and the subject or the object to be measured in the first sound wave detection procedure It is desirable to perform the first sound wave detection procedure once or more for the same subject or object to be measured.

  In the present invention, since the component concentration measurement is repeatedly performed on the same subject or object to be measured, it is possible to grasp the change with time of the component concentration state of the same object or object to be measured.

  In the component concentration measurement method of the present invention, after the first sound wave detection procedure, a standard sample different from the standard sample in the first sound wave detection procedure, and the subject or the object to be measured in the first sound wave detection procedure It is desirable to perform the first sound wave detection procedure once or more for the same subject or object to be measured.

  In the present invention, component concentration measurement is repeatedly performed on different standard samples, so that individual differences between the specimen or the object to be measured can be grasped.

  In the component concentration measurement method according to the present invention, the measurement light modulation means electrically modulates the intensity of two waves of the same wavelength with a signal having the same frequency and opposite phase, and the reference light modulation means outputs the measurement modulation means. The two light beams having the same wavelength and the two light beams having different wavelengths are electrically intensity-modulated with a signal having the same frequency as that of the intensity modulation frequency of the measurement light modulation means and an opposite phase signal, and output. The wave light emitting means combines two light beams of opposite phases among the two light lights from the measurement light modulating means and the two light lights from the reference light modulating means, and emits two lights, The sound wave detecting means is irradiated with the sound wave generated from the standard sample irradiated with one of the two lights from the combined light emitting means and the other light among the two lights from the combined light emitting means. The sound wave generated from the subject or the object to be measured is converted into the standard sample, The irradiated fine said light comprising a second acoustic wave detection procedure for detecting at the same position relative to the subject or the object to be measured.

  In the present invention, since the sound wave detecting means detects the sound wave at the same position, the magnitude of the sound wave generated by the two substances, that is, the subject or the object to be measured and the standard sample irradiated with the light from the light emitting means. Can be detected simultaneously. Therefore, it is possible to correct changes with time of the components of the standard sample in the subject or object to be irradiated with light. It is also possible to correct the influence of the components of the standard sample other than the measurement target in the subject or object to be irradiated with light. Furthermore, since the sound wave detecting means detects the sound wave at the same position, there is no sound measurement error due to individual differences of the sound wave detecting means. Therefore, in the present invention, the component concentration of the analyte or the analyte can be accurately measured as compared with the case where the two substances are separately measured and corrected after recording.

  In the component concentration measurement method of the present invention, the measurement light modulation means electrically modulates and outputs two waves of the same wavelength with signals of the same frequency and opposite phase, and the reference light modulation means outputs the measurement modulation. The light of two waves of the same wavelength and the two waves of different wavelengths of the means are electrically intensity-modulated with a signal having an opposite phase at the same frequency as the intensity-modulated frequency of the measurement light modulation means, and output. The combined light emitting unit combines two light beams of opposite phases from the two light beams from the measurement light modulating unit and the two light beams from the reference light modulating unit, respectively. Wavelength adjusting means for adjusting the wavelengths of the two light beams in the measurement light modulating means and the reference light modulating means so that the magnitude of the sound wave generated from the water becomes zero is emitted to water. Wavelength adjustment procedure to adjust the wavelength of light It is desirable to further comprise before the serial second acoustic wave detection procedure.

  In the present invention, the measurement light modulation unit and the reference light modulation unit include a wavelength adjustment procedure for adjusting the wavelength of the two light beams so that the size of the sound wave generated from the water becomes zero. The wavelengths of the two emitted lights can be set to predetermined two wavelengths having the same water absorbance characteristics. As a result, the influence of water absorbance can be removed in advance from the sound wave to be detected. Therefore, in the present invention, it is not necessary to correct the water absorbance component when calculating the component concentration of the subject to be measured or the object to be measured, and the measurement can be performed accurately.

  In the component concentration measurement method of the present invention, after the second sound wave detection procedure, the same standard sample as the standard sample in the second sound wave detection procedure, and the subject or the object to be measured in the second sound wave detection procedure It is desirable to perform the second sound wave detection procedure once or more for the same subject or object to be measured.

  In the present invention, since the component concentration measurement is repeatedly performed on the same subject or object to be measured, it is possible to grasp the change with time of the component concentration state of the same object or object to be measured.

  In the component concentration measurement method of the present invention, after the second sound wave detection procedure, the same standard sample as the standard sample different from the standard sample in the second sound wave detection procedure and the subject or the object to be measured in the second sound wave detection procedure It is desirable to perform the second sound wave detection procedure once or more for the subject or the object to be measured.

  In the present invention, component concentration measurement is repeatedly performed on different standard samples, so that individual differences between the specimen or the object to be measured can be grasped.

  The component concentration measuring apparatus and the component concentration measuring method of the present invention accurately calibrate the component concentration measurement by reducing the influence of the time-dependent change of the subject or object to be irradiated with light and the time-dependent change of the sound wave detector. be able to.

  Embodiments of the present invention will be described with reference to the accompanying drawings.

  The following embodiment is an example of the configuration of the present invention, and is an embodiment in the case where a component concentration is measured by a finger of a human body as a subject, but the present invention is limited to the following embodiment is not. In the following, an embodiment in which the concentration of a component of a measurement object is measured can be obtained by replacing the object with the measurement object. Also, in FIGS. 2 to 4 showing the configuration of the component concentration measuring apparatus according to each embodiment, portions that can be realized by a normal technique such as a power source or a control unit that controls the overall operation are not shown.

(First embodiment)
FIG. 2 shows a schematic configuration diagram of a component concentration measuring apparatus according to the present embodiment. Moreover, the schematic of the upper surface of the container which concerns on FIG. 5 at this embodiment is shown.

  In FIG. 2, the component concentration measuring apparatus 10 includes an oscillator 101, a drive circuit 103, a 180 ° phase shift circuit 102, a drive circuit 106, a first light source 104, and a second light source 107 as part of a light emitting unit. A sound wave detection unit 108 as a part of the sound wave detection unit, a preamplification unit 109 and a phase detection amplification unit 110, a component concentration calculation unit 111 as a sound wave recording unit and a component concentration calculation unit, and a standard sample 123 are provided. Moreover, in this embodiment, in order to improve the detection efficiency of a sound wave, the container 121 as an elliptical reflector is provided. In FIG. 2, for the container 121, a cut surface is shown for convenience.

  The oscillator 101 outputs a modulation signal for intensity-modulating light of two wavelengths output from the first light source 104 and the second light source 107. The 180 ° phase shift circuit 102 inverts one of the modulation signals from the oscillator 101 and outputs it.

The drive circuit 103 drives the first light source 104 based on the modulation signal from the oscillator 101. The drive circuit 106 drives the second light source 107 based on the modulation signal inverted by the 180 ° phase shift circuit 102. The first light source 104 modulates the intensity of one of the two different wavelengths of light with a signal from the drive circuit 103 and outputs the first light having the wavelength λ ₁ , and the second light source 107 outputs the other light. the by intensity-modulated by a signal from the drive circuit 106 to output a second light of the wavelength lambda _2. As a result, the first light source 104 and the second light source 107 can output the light of two different wavelengths that are electrically intensity-modulated with signals having the same frequency and opposite phase. Further, if the emission of light from either the first light source 104 or the second light source 107 is stopped, the first light source 104 or the second light source 107 has a predetermined one of two different wavelengths of light. Only light having a wavelength can be emitted.

  For example, a semiconductor laser can be applied to the first light source 104 and the second light source 107, and the wavelength of light generated by heating or cooling with a heater or a Peltier element can be changed. For example, each wavelength is set to a wavelength at which the component whose measurement target is the wavelength of one light exhibits characteristic absorption, and the wavelength of the other light is equal to that of water at the wavelength of one light. Is set to a wavelength exhibiting In the present embodiment, a rectangular wave signal is output from the oscillator 101, and the first light source 104 and the second light source 107, which are semiconductor laser light sources, are connected to each other at the same frequency via the drive circuits 103 and 106, respectively. The signal is directly modulated by an antiphase rectangular wave signal. In this way, by directly modulating each of the two semiconductor laser light sources with rectangular wave signals having the same frequency and opposite phases, it is possible to generate light of two different wavelengths and simultaneously modulate them, thereby simplifying the device configuration. Can be

  Here, the component concentration measuring apparatus 10 emits two waves of light from the first light source 104 and the second light source 107 to the water after being electrically intensity-modulated by signals of the same frequency and opposite phase. It is desirable to provide wavelength adjusting means (not shown) for adjusting the wavelengths of the two light beams so that the size of the sound wave generated from the laser beam becomes zero. The wavelength adjusting means adjusts the wavelength of the two light beams, so that the wavelengths of the two light beams emitted from the first light source 104 and the second light source 107 are two predetermined wavelengths that match the water absorbance characteristics. be able to. Thereby, the influence of the water absorbance can be removed in advance from the sound wave propagating to the sound wave detection unit 108. Therefore, the component concentration measuring apparatus 10 does not need to correct the absorbance component of water when calculating the component concentration of the subject to be measured or the object to be measured, and can accurately measure the concentration.

  In addition, the component concentration measurement apparatus 10 may adjust the light intensity of the two waves so that the first light source 104 and the second light source 107 have zero sound wave generated from water. As a result, the light intensities of the two waves emitted from the first light source 104 and the second light source 107 can be made substantially equal. Therefore, the component concentration of the measurement target of the subject 2 can be accurately measured.

  Furthermore, the component concentration measuring apparatus 10 emits two light beams having different wavelengths from the first light source 104 and the second light source 107 by electrically modulating the intensity with two signals having the same frequency. The phase of the two signals having the same frequency may be adjusted so that the magnitude of the sound wave generated from water becomes zero. That is, in this embodiment, one of the modulation signals output from the drive circuits 103 and 106 is delayed for a predetermined time to adjust the phase of the modulation signals. By adjusting the phases of the two signals in this way, the phases of the electrical signals that modulate the light emitted from the first light source 104 and the second light source 107 can be made opposite to each other. It is possible to accurately measure the concentration of the component to be measured.

  The standard sample 123 is irradiated with light from the second light source 107. As the standard sample 123, a bio-equivalent substance prepared so as to exhibit absorbance, electrothermal characteristics, and acoustic characteristics equivalent to the subject 2 is enclosed. An example is a softened gel-like sample containing moisture. It is also possible to enclose blood or serum whose blood component amount has been tested. Examples thereof include blood collected from a living body in advance and serum obtainable from the blood. The component concentration measuring apparatus 10 can correct the component concentration measurement value of the standard sample 123 with water, blood, serum, or a biological equivalent substance according to the component to be measured by the subject 2. The component concentration can be accurately measured.

  As shown in FIGS. 2 and 5, the container 121 has two concave ellipsoidal surfaces 124 and 125 that can reflect sound waves generated by the subject 2 and the standard sample 123. Either one is arranged with a common focal point 132. In the present embodiment, the container 121 is filled with an acoustic matching substance having the acoustic impedance of the subject 2, and the subject 2, the sound wave detection unit 108, and the standard sample 123 are disposed on the opening side of the container 121. The lid 120 is provided so that the can be arranged. The subject 2, the sound wave detection unit 108, and the standard sample 123 arranged on the lid 120 have the subject 2 as the first focus 131, the standard sample 123 as the second focus 133, and the sound wave detection unit 108. Arranged at the focal point 132, respectively.

  Moreover, the other form of a container is shown in FIG.

  In the container 122 shown in FIG. 6, three concave elliptical spheres 126, 127, and 128 are arranged with one of the two focal points of the respective elliptical spheres 126, 127, and 128 as a common focal point 134. The subject 2 is arranged at the first focal point 137, the standard sample 151 is arranged at the second focal point 135, and the standard sample 152 is arranged at the third focal point 136. Arranged in this way, the standard samples 151 and 152 are irradiated with light from the second light source 107 shown in FIG. 2 respectively, or the standard samples 151 and 152 are alternately irradiated with light from the second light source 107. Thus, correction based on two different standard samples becomes possible.

  The sound wave detection unit 108 in FIG. 2 detects the sound wave generated in the subject 2 by the light from the first light source 104 and the sound wave generated in the standard sample 123 by the light from the second light source 107 at the same position. In the present embodiment, since the sound wave detection unit 108 is placed at the same position and arranged at the common focal point 132 of the container 121 having the elliptical spherical surfaces 124 and 125, the sound wave is detected. Sound waves 141 and 142 generated at the first focal point 131 and the second focal point 133 can be collected at the common focal point 132. Therefore, in the component concentration measuring apparatus 10, the sound wave detection unit 108 can reduce detection loss of sound waves and detect sound waves with high sensitivity, and can accurately measure the component concentration of the subject 2. In the present embodiment, since the sound wave detection unit 108 is disposed at the common focal point 132, the sound wave detection unit 108 is disposed at a substantially equidistant position from the subject 2 and the standard sample 123. Therefore, the attenuation of the sound waves 141 and 142 generated in the subject 2 and the standard sample 123 with respect to the propagation distance is substantially the same, so that no measurement error of the component concentration measurement due to the attenuation of the sound waves occurs. Furthermore, since the inside of the container 121 is filled with the acoustic matching substance having the acoustic impedance of the subject 2, the transmission efficiency of the sound wave 141 generated from the subject 2 can be improved. Therefore, attenuation due to the propagation distance of the sound wave can be reduced and the sound wave detection unit 108 can detect the sound wave with high sensitivity, and the component concentration of the subject 2 can be accurately measured. In the present embodiment, the sound wave detection unit 108 detects the sound wave by placing it at the common focus 132 at the same position, but simultaneously detects the sound wave 141 generated by the subject 2 and the sound wave 142 generated by the standard sample 123. If possible, it may be detected at any position.

  The preamplifier 109 amplifies the sound wave from the sound wave detector 108 and outputs it. The phase detection amplification unit 110 synchronously detects the electric signal from the preamplification unit 109 using the modulation signal from the oscillator 101, and outputs an electric signal proportional to the sound pressure. Thus, by detecting the sound wave by synchronous detection synchronized with the modulation frequency, the sound wave can be detected with high accuracy.

  The component concentration calculation unit 111 stores temporal changes in the size of sound waves detected by the sound wave detection unit 108 and output via the preamplification unit 109 and the phase detection amplification unit 110. Is calculated. As will be described later, the component concentration measuring apparatus 10 can record the time variation of the sound wave intensity in the component concentration calculating unit 111, and thus can correct the component concentration measurement value based on the recorded value of the sound wave intensity. The component concentration of the measurement target of the subject 2 can be accurately measured.

  As described above, the component concentration measuring apparatus 10 detects the sound wave at the same position, so that the subject 2 irradiated with the light from the first light source 104 and the second light source 107 is used. And the standard sample 123 can simultaneously detect the difference in the magnitude of sound waves generated by the two substances. Therefore, as will be described later, it is possible to correct a change with time in the components of the standard sample 123 in the subject 2 irradiated with light. Further, the influence of the components of the standard sample 123 other than the measurement target in the subject 2 irradiated with light can be corrected. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, the component concentration measuring apparatus 10 can accurately measure the component concentration of the subject 2 as compared to measuring two substances separately and correcting them after recording.

  Next, the component concentration measurement operation of the component concentration measuring apparatus 10 according to the present embodiment will be described with reference to FIG.

  The component concentration measuring apparatus 10 performs the following operations as a wavelength adjustment procedure and a phase adjustment procedure.

  First, instead of the subject 2 in FIG. 2, water as an object to be measured is arranged at the first focal point 131, and water as the standard sample 123 is arranged at the second focal point 133. The drive circuit 103 outputs a signal for driving the first light source 104 in synchronization with the modulation signal from the oscillator 101. Further, the drive circuit 106 outputs a signal for driving the second light source 107 in synchronization with the modulation signal via the 180 ° phase shift circuit 102. The first light source 104 electrically modulates and outputs light with a signal from the drive circuit 103, and the second light source 107 electrically modulates and outputs light with a signal from the drive circuit 106. To do. The sound wave detection unit 108 detects sound waves generated from water disposed at the first focal point 131 and the second focal point 133 by the light from the first light source 104 and the second light source 107.

  Based on the sound wave detected in this way, the first light source 104 and the second light source 107 adjust the wavelength of each other light so that the sound wave becomes zero. In addition, the drive circuits 103 and 106 can adjust the phases of the signals output to the first light source 104 and the second light source 107 so that the sound wave becomes zero. In addition, the first light source 104 and the second light source 107 can adjust the light intensity of each other so that the sound wave becomes zero. Thus, the wavelength of the two light beams emitted from the first light source 104 and the second light source 107 is adjusted by adjusting the wavelength of the two light beams so that the magnitude of the sound wave generated from the water becomes zero. Can be made to have two predetermined wavelengths that match the water absorption characteristics. Further, the light emitted from the first light source 104 and the second light source 107 is adjusted by adjusting the phase of the signal of the same frequency from the drive circuits 103 and 106 so that the magnitude of the sound wave generated from water becomes zero. The phases of the electrical signals that modulate the signal can be opposite to each other. Further, by adjusting the light intensity of the two waves so that the size of the sound wave becomes zero, the light intensity of the two waves emitted from the first light source 104 and the second light source 107 is made substantially equal. be able to. Therefore, the component concentration measuring apparatus 10 can accurately measure the component concentration of the measurement target of the subject 2.

  After adjusting the operation states of the first light source 104, the second light source 107, and the drive circuits 103 and 106 as described above, the component concentration measurement apparatus 10 performs the following operation as the first sound wave detection procedure.

  In place of the subject 2 in FIG. 2, a bioequivalent substance as an object to be measured is disposed at the first focal point 131, and the bioequivalent substance identical to the bioequivalent substance disposed at the first focal point 131 is disposed at the second focal point 133. An embodiment in the case of being arranged in will be described.

  After the bioequivalent material is disposed at the first focal point 131 and the bioequivalent material that is the same as the bioequivalent material disposed at the first focal point 131 is disposed at the second focal point 133, the drive circuit 103 is connected to the oscillator 101. A signal for driving the first light source 104 is output in synchronization with the modulation signal. Further, the drive circuit 106 outputs a signal for driving the second light source 107 in synchronization with the modulation signal via the 180 ° phase shift circuit 102. Here, the signal output from the drive circuit 103 and the signal output from the drive circuit 106 are inverted from each other. The first light source 104 electrically modulates and outputs light with a signal from the drive circuit 103, and the second light source 107 electrically modulates and outputs light with a signal from the drive circuit 106. To do. The sound wave detection unit 108 detects, at the same position, sound waves generated from the bioequivalent substances disposed at the first focus 131 and the second focus 133 by the light from the first light source 104 and the second light source 107. To do.

  Here, the sound wave detection unit 108 detects sound waves generated from the bioequivalent material disposed at the first focal point 131 and the second focal point 133 and reflected by the elliptical spherical surfaces 124 and 125 in the container 121. Therefore, due to the elliptical nature, sound waves generated at the first focal point 131 and the second focal point 133 can be collected at the common focal point 132. Therefore, the sound wave detection unit 108 can detect sound waves with high sensitivity by reducing detection loss of sound waves. In the component concentration measurement method according to the present embodiment, the component concentration of the bioequivalent substance as the measurement object can be accurately measured. In the present embodiment, since the sound wave detection unit 108 is disposed at the common focal point 132, the sound wave detection unit 108 is disposed at a substantially equidistant position from the biological equivalent material as the measurement target and the biological equivalent material as the standard sample 123. It becomes. Therefore, the attenuation with respect to the propagation distance of the sound waves 141 and 142 generated by the biological equivalent material as the measurement object and the biological equivalent material as the standard sample 123 is substantially the same. It does not occur. Furthermore, if the acoustic matching material of the acoustic impedance of the biological equivalent material as the measurement object is filled in the container 121, the transmission efficiency of the sound wave 141 generated from the biological equivalent material as the measurement object can be improved. . Therefore, attenuation due to the propagation distance of the sound wave can be reduced and the sound wave detection unit 108 can detect the sound wave with high sensitivity, and the component concentration of the subject 2 can be accurately measured.

  The component concentration calculation unit 111 records a temporal change in the size of the sound wave detected by the sound wave detection unit 108, amplified by the preamplification unit 109, and detected by the phase detection amplification unit 110. As described above, the biological equivalent substance is a substance prepared so as to exhibit absorbance, electrothermal characteristics, and acoustic characteristics equivalent to those of the subject 2. For example, the biological equivalent substance is formed by irradiating light of a sound wave generated from the biological equivalent substance. The change with time of the equivalent substance accompanying the temperature change is equivalent to the subject 2. Therefore, if the temporal change of the sound wave intensity is recorded as in the present embodiment, the component concentration measurement value can be corrected based on the recorded value of the sound wave intensity. That is, after recording the time change of the sound wave generated from the biological equivalent material, the subject 2 is placed at the first focal point 131 and the second focal point 133 instead of the biological equivalent material, and the first light source 104 and the first light source 104 A sound wave is detected by irradiating light from the second light source 107, and the magnitude of the sound wave is recorded in the component concentration calculation unit 111. Thereafter, a sound wave obtained by subtracting a time change of the sound wave generated in the biological equivalent substance from the sound wave generated in the subject 2 can be corrected.

  As another form, blood as an object to be measured is placed at the first focus 131 instead of the subject 2 in FIG. 2, and the same blood as the blood placed at the first focus 131 is placed at the second focus 133. The same procedure as the first sound wave detection procedure may be performed. If the temporal change of the sound wave intensity of the sound wave generated in the blood disposed at the first focal point 131 and the second focal point 133 is recorded, the component concentration measurement value can be corrected based on the recorded sound wave intensity value. That is, the time change of the sound wave generated from the blood can be recorded as a difference in absorbance of the blood with respect to the light from the first light source 104 and the second light source 107. Therefore, after recording the difference in absorbance, the subject 2 is placed at the first focal point 131 and the second focal point 133 instead of blood, and light is emitted from the first light source 104 and the second light source 107. A sound wave is detected, and the component concentration calculator 111 records the magnitude of the sound wave. Thereafter, it can be corrected as a sound wave obtained by subtracting the difference in absorbance in blood from the sound wave generated in the subject 2.

  When the container 122 shown in FIG. 6 is used instead of the container 121, the bioequivalent substance and blood in the above two embodiments are disposed at the second focal point 135 and the third focal point 136, respectively, and the second focal point 135 and By alternately irradiating the light from the second light source 107 to the standard samples 151 and 152 arranged at the third focal point 136, correction based on the biological equivalent substance and blood is possible without changing the arrangement of the standard samples. It becomes.

  As described above, in the component concentration measurement method according to this embodiment, since the sound wave detection unit 108 detects sound waves at the same position, the biological equivalent as a measurement object irradiated with the light from the first light source 104 is used. It is possible to simultaneously detect the difference in the magnitude of sound waves generated by the substance, blood, and the biological equivalent substance as a standard sample irradiated with light from the second light source 107 and the two substances of blood. Therefore, it is possible to correct a change with time of a biological equivalent substance or a blood component in the subject 2 irradiated with light. It is also possible to correct the influence of biological equivalent substances and blood components that are components other than the measurement target in the subject 2 irradiated with light. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, in the component concentration measurement method according to the present embodiment, the component concentration of the subject 2 can be accurately measured as compared to measuring two substances separately and correcting them after recording.

  In addition, after the first sound wave detection procedure, the same standard sample as the biological equivalent substance or blood as the standard sample in the first sound wave detection procedure, and the biological equivalent substance or blood as the measurement object in the first sound wave detection procedure It is desirable to perform the first sound wave detection procedure once or more for the same object to be measured. Since the component concentration measurement is repeatedly performed on the same object to be measured, it is possible to grasp the change with time of each measurement of the state of the biological equivalent substance or blood component concentration as the same object to be measured.

  In addition, after the first sound wave detection procedure, a standard sample different from a biological equivalent substance or blood as a standard sample in the first sound wave detection procedure, and a biological equivalent substance or blood as a measurement object in the first sound wave detection procedure It is desirable to perform the first sound wave detection procedure once or more for the object to be measured. Since the component concentration measurement is repeatedly performed on different standard samples, it is possible to grasp individual differences in a biological equivalent substance or blood as a measurement object.

(Second Embodiment)
A component concentration measuring apparatus according to the second embodiment will be described.

  FIG. 3 shows a schematic configuration diagram of the component concentration measuring apparatus according to the present embodiment. In FIG. 3, the same constituent elements as those in the first embodiment have the same constituent reference numerals. In addition, two or more components having the same component code are assigned different English codes such as the first light sources 104a and 104b.

  The component concentration measuring apparatus 11 according to the present embodiment is configured to emit light having the same wavelength as that of the first light source 104 instead of the second light source 107 in the component concentration measuring apparatus 10 shown in FIG. Therefore, the component concentration measuring apparatus 11 irradiates the subject 2 with light from the first light source 104 a that is electrically intensity-modulated by a signal from the drive circuit 103, and is electrically intensified by a signal from the drive circuit 106. The standard sample 123 is irradiated with light from the modulated first light source 104b. Note that the signal from the drive circuit 103 and the signal from the drive circuit 106 have opposite phases.

  Here, the component concentration measuring device 11 emits light of two waves having the same wavelength from the first light source 104a and the first light source 104b by electrically modulating the intensity with two signals of the same frequency, respectively. It is desirable to adjust the phase of the two signals having the same frequency so that the magnitude of the sound wave generated from water becomes zero. That is, in this embodiment, one of the modulation signals output from the drive circuits 103 and 106 is delayed for a predetermined time to adjust the phase of the modulation signals. By adjusting the phases of the two signals in this way, the phases of the electrical signals that modulate the light emitted from the first light source 104a and the first light source 104b can be made opposite to each other. It is possible to accurately measure the concentration of the component to be measured.

  In the component concentration measuring apparatus 11, since the sound wave detection unit 108 detects a sound wave at the same position, the subject 2 and the standard sample 123 irradiated with light from the first light source 104a and the first light source 104b. The difference in the magnitude of sound waves generated by the two substances can be detected simultaneously. Therefore, as will be described later, it is possible to correct a change with time in the components of the standard sample 123 in the subject 2 irradiated with light. Further, the influence of the components of the standard sample 123 other than the measurement target in the subject 2 irradiated with light can be corrected. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, the component concentration measuring apparatus 11 can accurately measure the component concentration of the subject 2 as compared to measuring two substances separately and correcting them after recording.

  Next, the component concentration measurement operation of the component concentration measuring apparatus 11 according to the present embodiment will be described with reference to FIG.

  The component concentration measuring apparatus 11 performs the following operations as a wavelength adjustment procedure and a phase adjustment procedure.

  First, instead of the subject 2 in FIG. 3, water as the object to be measured is arranged at the first focal point 131, and water as the standard sample 123 is arranged at the second focal point 133. Then, the driving circuit 103 outputs a signal for driving the first light source 104 a in synchronization with the modulation signal from the oscillator 101. Further, the drive circuit 106 outputs a signal for driving the first light source 104b in synchronization with the modulation signal via the 180 ° phase shift circuit 102. The first light source 104a electrically modulates and outputs light with the signal from the drive circuit 103, and the first light source 104b outputs light with the signal intensity modulated with the signal from the drive circuit 106. To do. The sound wave detection unit 108 detects sound waves generated from water disposed at the first focal point 131 and the second focal point 133 by the light from the first light source 104a and the first light source 104b.

  Based on the sound wave detected in this way, the first light source 104a and the first light source 104b adjust the wavelength of each other light so that the sound wave becomes zero. In addition, the drive circuits 103 and 106 can adjust the phases of the signals output to the first light source 104a and the first light source 104b so that the sound wave becomes zero. Further, the first light source 104a and the first light source 104b can also adjust the light intensity of each other so that the sound wave becomes zero. In this way, the wavelength of the two light beams emitted from the first light source 104a and the first light source 104b is adjusted by adjusting the wavelength of the two light beams so that the magnitude of the sound wave generated from the water becomes zero. Can be the same. Further, the light emitted from the first light source 104a and the first light source 104b is adjusted by adjusting the phase of the signals having the same frequency in the drive circuits 103 and 106 so that the magnitude of the sound wave generated from water becomes zero. The phases of the electric signals to be modulated can be opposite to each other. Further, by adjusting the light intensity of the two waves so that the magnitude of the sound wave becomes zero, the light intensity of the two waves emitted from the first light source 104a and the first light source 104b is made substantially equal. be able to. Therefore, the component concentration measuring apparatus 11 can accurately measure the component concentration of the measurement target of the subject 2.

  After adjusting the operation states of the first light source 104a, the first light source 104b, and the drive circuits 103 and 106 as described above, the component concentration measurement apparatus 11 performs the following operation as the first sound wave detection procedure.

  An embodiment in which the subject 2 in FIG. 3 is arranged at the first focal point 131 and water as the standard sample 123 is arranged at the second focal point 133 will be described.

  After the subject 2 is arranged at the first focal point 131 and water as the standard sample 123 is arranged at the second focal point 133, the drive circuit 103 synchronizes with the modulation signal from the oscillator 101 and the first light source 104a. The signal which drives is output. Further, the drive circuit 106 outputs a signal for driving the first light source 104b in synchronization with the modulation signal via the 180 ° phase shift circuit 102. Here, the signal output from the drive circuit 103 and the signal output from the drive circuit 106 are inverted from each other. The first light source 104a electrically modulates and outputs light with the signal from the drive circuit 103, and the first light source 104b outputs light with the signal intensity modulated with the signal from the drive circuit 106. To do. The sound wave detection unit 108 uses the light from the first light source 104 a and the first light source 104 b as water as the subject 2 arranged at the first focal point 131 and the standard sample 123 arranged at the second focal point 133. The sound wave generated from is detected at the same position.

  Also in the present embodiment, the sound wave detection unit 108 is generated from the water as the standard sample 123 disposed at the subject 2 and the second focal point 133 disposed at the first focal point 131, and is stored in the container 121. In order to detect sound waves reflected by the elliptic spherical surfaces 124 and 125, the sound waves generated at the first focal point 131 and the second focal point 133 are common focal point 132 due to the nature of the ellipse as described in the first embodiment. Therefore, it is possible to detect the sound wave with high sensitivity by reducing the detection omission of the sound wave, and to accurately measure the component concentration of the subject 2. Further, since the sound wave detection unit 108 is disposed at the common focal point 132, the sound wave detection unit 108 is disposed at a substantially equidistant position from the subject 2 and the standard sample 123. For this reason, the attenuation with respect to the propagation distance of the sound waves 141 and 142 generated in water as the subject 2 and the standard sample 123 is substantially the same, so that a measurement error of the component concentration measurement accompanying the attenuation of the sound waves does not occur. Furthermore, if the inside of the container 121 is filled with the same acoustic matching material as the acoustic impedance of the subject 2, the transmission efficiency of the sound wave 141 generated from the subject 2 can be improved. Therefore, attenuation due to the propagation distance of the sound wave can be reduced and the sound wave detection unit 108 can detect the sound wave with high sensitivity, and the component concentration of the subject 2 can be accurately measured.

  The component concentration calculation unit 111 records a temporal change in the size of the sound wave detected by the sound wave detection unit 108, amplified by the preamplification unit 109, and detected by the phase detection amplification unit 110. If the temporal change of the sound wave intensity is recorded as in this embodiment, the component concentration measurement value can be corrected based on the sound wave intensity recorded value. It is possible to correct a sound wave obtained by subtracting a time change of a sound wave generated in water from a sound wave generated in the subject 2.

Here, sound waves s ₂ from the water as waves s ₁ and a standard sample 123 from the subject 2, since the material composition of the subject 2 and water are different, occurs as each other different values. The sound wave s ₁ and the sound wave s ₂ can be expressed by Equation (4).

In the formula (4), the wavelengths of light from the first light source 104a and the first light source 104b are equal, so in the formula (1), α ₁ ^(w) = α ₁ ^(w) and the standard sample 123 is water and This is equivalent to α ₂ ^(g) = 0. Therefore, in Formula (2), if α ₁ ^(w) = α ₁ ^(w) and α ₂ ^(g) = 0, and separately detecting the sound wave s ₂ generated only by the light from the first light source 104b, The component concentration M can be measured.

As another form, the first sound wave detection procedure may be performed using a biological equivalent material as the standard sample 123 instead of the water as the standard sample 123 in FIG. In this case, the sound wave s _1t generated from the biological equivalent material as the standard sample 123 by the light from the first light source 104b.
The sound wave s _1h generated from the subject 2 by the light from the first light source 104a can be expressed as Equation (5).

  Here, if C in Formula (5) is deleted from Formula (5), it can be expressed as Formula (6), and the component concentration M can be calculated.

In the present embodiment, the change in α ₁ ^(w ) in Equation (6) is less than 1% at 1 ° C., and the measurement site does not change by 5 degrees or more due to heating by the laser. It is considered that there will be no problem with the density measurement accuracy.

  When the container 122 shown in FIG. 6 is used instead of the container 121, water and a bioequivalent substance as standard samples in the above two embodiments are disposed at the second focal point 135 and the third focal point 136, respectively. By alternately irradiating the light from the first light source 104b to the standard samples 151 and 152 arranged at the focal point 135 and the third focal point 136, the standard sample is placed on the basis of water and a biological equivalent substance without changing the arrangement of the standard sample. Correction becomes possible.

  As described above, in the component concentration measurement method according to the present embodiment, since the sound wave detection unit 108 detects sound waves at the same position, the subject is irradiated with light from the first light source 104a and the first light source 104b. 2 and the difference in the magnitude of sound waves generated by two substances of water as the standard sample 123 can be detected simultaneously. Therefore, it is possible to correct a change with time of moisture or a component of the biological equivalent substance in the subject 2 irradiated with light. It is also possible to correct the influence of moisture or a component of a biological equivalent substance as a component other than the measurement target in the subject 2 irradiated with light. Further, it is possible to correct a change with time in the subject 2 irradiated with light. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, in the component concentration measurement method according to the present embodiment, the component concentration of the subject 2 can be accurately measured as compared to measuring two substances separately and correcting them after recording.

  Further, the standard sample 123 is, for example, blood collected in advance from the subject 2, and after performing the first sound wave detection procedure, the standard sample 123 and the first sound wave that are the same as the blood as the standard sample in the first sound wave detection procedure are used. It is desirable to perform the first sound wave detection procedure once or more for the same subject 2 as the subject 2 in the detection procedure. Since the component concentration measurement is repeatedly performed on the same subject 2, it is possible to grasp the temporal change for each measurement of the component concentration state of the same subject 2.

  Further, the standard sample 123 is blood collected in advance from the subject 2, for example, and after the first sound wave detection procedure, the standard sample different from the blood as the standard sample 123 in the first sound wave detection procedure, and in the first sound wave detection procedure It is desirable to perform the first sound wave detection procedure once or more for the same subject 2 as the subject 2. Since component concentration measurement is repeatedly performed on different standard samples 123, individual differences of the subject 2 can be grasped.

(Third embodiment)
A component concentration measuring apparatus according to the third embodiment will be described.

  FIG. 4 shows a schematic configuration diagram of the component concentration measuring apparatus according to the present embodiment. In FIG. 4, the same constituent elements as those in the first embodiment have the same constituent element numbers. In addition, two or more components having the same component code are assigned different English codes such as the first light sources 104a and 104b.

  The component concentration measurement apparatus 12 according to the present embodiment further combines light having a wavelength different from that of the light from the first light source 104 with the light from the first light source 104 in the component concentration measurement apparatus 10 illustrated in FIG. In the component concentration measuring apparatus 10, the light from the second light source 107 is further combined with light having a wavelength different from that of the light from the second light source 107. Accordingly, the component concentration measuring apparatus 12 includes the light from the first light source 104a and the second light source 107a that have been electrically intensity-modulated by signals opposite in phase by the drive circuits 103 and 106 and the 180 ° phase shift circuit 102. The light from the first light source 104b and the second light source 107b which are electrically intensity-modulated by the drive circuits 103 and 106 and the 180 ° phase shift circuit 102 by signals having opposite phases to each other. The standard sample 123 is irradiated with light from.

  In addition, the component concentration measuring apparatus 12 multiplexes the light from the first light source 104a and the light from the second light source 107a by the multiplexing unit 105a and irradiates the subject 2 with it. Further, the light from the first light source 104b and the light from the second light source 107b are combined by the combining unit 105b and irradiated to the standard sample 123. The multiplexing unit 105a combines the light from the first light source 104a and the light from the second light source 107a with, for example, a half mirror and emits the light toward the subject 2. Further, the multiplexing unit 105b combines the light from the first light source 104b and the light from the second light source 107b with, for example, a half mirror and emits the light toward the standard sample 123.

  In this embodiment, the oscillator 101, the drive circuits 103 and 106, the first light source 104a, and the first light source 104b have a function as measurement light modulation means. The oscillator 101, the 180 ° phase shift circuit 102, the drive circuits 103 and 106, the second light source 107a, and the second light source 107b function as reference light modulation means. Further, the multiplexing sections 105a and 105b have a function as a combined light emitting means.

  Here, the component concentration measuring apparatus 12 combines the two light beams from the first light source 104a and the second light source 107a by electrically modulating the intensity of the two light beams with the signals having the same frequency and the opposite phase, and emitting them to water. Then, the two light waves from the first light source 104b and the second light source 107b are electrically intensity-modulated by signals of the same frequency and opposite phase, combined and emitted to water, and sound waves generated from the water, respectively. It is desirable to provide wavelength adjusting means (not shown) for making the wavelength of each of the two waves so that the size of the light becomes zero. The wavelength adjustment means adjusts the wavelength, whereby the wavelengths of the two light beams emitted from the first light source 104a and the second light source 107a and the two light beams emitted from the first light source 104b and the second light source 107b. Can be made to have two predetermined wavelengths that match the water absorption characteristics. Thereby, the influence of the water absorbance can be removed in advance from the sound wave propagating to the sound wave detection unit 108. Therefore, the component concentration measuring device 12 does not need to correct the water absorbance component when calculating the component concentration of the subject to be measured or the object to be measured, and can accurately measure the concentration.

  In addition, the component concentration measuring device 12 may adjust the light intensity of the two waves so that the size of the sound wave generated from water becomes zero. Thereby, the light intensities of the two light beams emitted from the first light source 104a and the second light source 107a and the two light beams emitted from the first light source 104b and the second light source 107b can be made substantially equal. it can. Therefore, the component concentration of the measurement target of the subject 2 can be accurately measured.

  In addition, the component concentration measuring device 12 electrically modulates the intensity of two light beams having different wavelengths from the first light source 104a and the second light source 107a with two signals having the same frequency, and emits them to water. The two light beams having different wavelengths from the first light source 104b and the second light source 107b are electrically intensity-modulated by signals of the same frequency, combined and output to water, and the sound waves generated from the water are respectively output. The phases of the two signals having the same frequency may be adjusted so that the magnitude becomes zero. That is, in this embodiment, one of the modulation signals output from the drive circuits 103 and 106 is delayed for a predetermined time to adjust the phase of the modulation signals. Thus, by adjusting the phase of the two signals, the phase of the electric signal that modulates the two-wave light emitted from the first light source 104a and the second light source 107a, and the first light source 104b and the second light source. The phases of the electrical signals that modulate the two waves of light emitted from 107b can be made opposite to each other, and the component concentration of the measurement target of the subject 2 can be accurately measured.

  In the component concentration measuring device 12, the sound wave detection unit 108 detects sound waves at the same position, and therefore the subject 2 and the first light source irradiated with the mixed light from the first light source 104a and the second light source 107a. The difference in the magnitude of sound waves generated by the two substances from the standard sample 123 irradiated with the mixed light from 104b and the second light source 107b can be detected simultaneously. Therefore, as will be described later, it is possible to correct a change with time in the components of the standard sample 123 in the subject 2 irradiated with light. Further, the influence of the components of the standard sample 123 other than the measurement target in the subject 2 irradiated with light can be corrected. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, the component concentration measuring apparatus 12 can accurately measure the component concentration of the subject 2 as compared with the case where the two substances are separately measured and corrected after recording.

  Next, the component concentration measurement operation of the component concentration measuring apparatus 12 according to the present embodiment will be described with reference to FIG. The component concentration measuring apparatus 12 performs the following operations as a wavelength adjustment procedure and a phase adjustment procedure.

  First, instead of the subject 2 in FIG. 4, water as a measurement object is disposed at the first focal point 131, and water is disposed as the standard sample 123 at the second focal point 133. Then, the driving circuit 103 outputs signals for driving the first light source 104a and the second light source 107b in synchronization with the modulation signal from the oscillator 101. The driving circuit 106 outputs a signal for driving the first light source 104b and the second light source 107a in synchronization with the modulation signal via the 180 ° phase shift circuit 102. The first light sources 104a and 104b electrically modulate the intensity of the light according to the signals from the drive circuits 103 and 106 and output the light, and the second light sources 107a and 107b output a 180 ° phase shift circuit from the drive circuits 103 and 106. The light is electrically intensity-modulated by a signal via 102 and output. The sound wave detection unit 108 uses the mixed light from the first light source 104a and the second light source 107a and the mixed light from the first light source 104b and the second light source 107b to generate a first focal point 131 and a second focal point 133. The sound wave generated from the water placed in the is detected.

  Based on the sound waves detected in this way, the wavelengths of light in the first light sources 104a and 104b and the second light sources 107a and 107b are adjusted so that the sound waves become zero. In addition, the drive circuits 103 and 106 can adjust the phases of the signals output to the first light sources 104a and 104b and the second light sources 107a and 107b so that the sound wave becomes zero. The first light sources 104a and 104b and the second light sources 107a and 107b can also adjust the light intensity of each other so that the sound wave becomes zero. In this way, the two waves emitted from the first light sources 104a and 104b and the second light sources 107a and 107b are adjusted by adjusting the wavelengths of the two light beams so that the size of the sound wave generated from the water becomes zero. The wavelength of the light can be set to two predetermined wavelengths in which the absorbance characteristics of water coincide with each other in advance. Further, the first light sources 104a and 104b and the second light sources 107a and 107b are adjusted by adjusting the phases of the signals having the same frequency from the drive circuits 103 and 106 so that the magnitude of the sound wave generated from the water becomes zero. The phases of the electrical signals that modulate the light emitted from can be made opposite to each other. Further, the light intensity of the two waves emitted from the first light sources 104a and 104b and the second light sources 107a and 107b is adjusted by adjusting the light intensity of the two waves so that the size of the sound wave becomes zero. Can be made substantially equal. Therefore, the component concentration measuring device 12 can accurately measure the component concentration of the measurement target of the subject 2.

  After adjusting the operation states of the first light sources 104a and 104b, the second light sources 107a and 107b, and the drive circuits 103 and 106 as described above, the component concentration measuring apparatus 12 performs the following operation as the second sound wave detection procedure. To do.

  An embodiment in which the subject 2 in FIG. 4 is disposed at the first focal point 131 and blood as the standard sample 123 is disposed at the second focal point 133 will be described.

  After the subject 2 is disposed at the first focal point 131 and the blood as the standard sample 123 is disposed at the second focal point 133, the drive circuit 103 synchronizes with the modulation signal from the oscillator 101 and the first light source 104a. And a signal for driving the second light source 107b. The driving circuit 106 outputs a signal for driving the first light source 104b and the second light source 107a in synchronization with the modulation signal via the 180 ° phase shift circuit 102. Here, the signal output from the drive circuit 103 and the signal output from the drive circuit 106 are inverted from each other. The first light source 104a electrically modulates the intensity of light according to a signal from the drive circuit 103 and outputs the light, and the second light source 107a emits light according to a signal from the drive circuit 106 via the 180 ° phase shift circuit 102. Electrically intensity modulated and output. The second light source 107b electrically modulates the intensity of the light according to the signal from the driving circuit 103 and outputs the light, and the first light source 104b outputs the signal from the driving circuit 106 via the 180 ° phase shift circuit 102. The light is intensity-modulated and output. The sound wave detection unit 108 is a subject arranged at the first focal point 131 by the mixed light from the first light source 104a and the second light source 107a and the mixed light from the first light source 104b and the second light source 107b. Sound waves generated from blood as the standard sample 123 arranged at the second and second focal points 133 are detected at the same position.

  Also in this embodiment, the sound wave detection unit 108 is generated from blood as the standard sample 123 disposed at the subject 2 and the second focal point 133 disposed at the first focal point 131, and is stored in the container 121. In order to detect sound waves reflected by the elliptic spherical surfaces 124 and 125, the sound waves generated at the first focal point 131 and the second focal point 133 are common focal point 132 due to the nature of the ellipse as described in the first embodiment. Therefore, it is possible to detect the sound wave with high sensitivity by reducing the detection omission of the sound wave, and to accurately measure the component concentration of the subject 2. Further, since the sound wave detection unit 108 is disposed at the common focal point 132, the sound wave detection unit 108 is disposed at a substantially equidistant position from the subject 2 and the standard sample 123. For this reason, the attenuation with respect to the propagation distance of the sound waves 141 and 142 generated in the blood as the subject 2 and the standard sample 123 is substantially the same, so that a measurement error of the component concentration measurement accompanying the attenuation of the sound waves does not occur. Furthermore, if the inside of the container 121 is filled with the same acoustic matching material as the acoustic impedance of the subject 2, the transmission efficiency of the sound wave 141 generated from the subject 2 can be improved. Therefore, attenuation due to the propagation distance of the sound wave can be reduced and the sound wave detection unit 108 can detect the sound wave with high sensitivity, and the component concentration of the subject 2 can be accurately measured.

  The component concentration calculation unit 111 records a temporal change in the size of the sound wave detected by the sound wave detection unit 108, amplified by the preamplification unit 109, and detected by the phase detection amplification unit 110. If the temporal change of the sound wave intensity is recorded as in this embodiment, the component concentration measurement value can be corrected based on the sound wave intensity recorded value. That is, the time change of the sound wave generated in the blood from the sound wave generated in the subject 2 can be corrected.

Here, the absorption coefficient of the subject 2 with respect to the light from the first light source 104a is α ₁ ^(w) + M′α ₁ ^(b) (where M ′ is the unknown concentration of the component other than the measurement component and its absorbance α). ₁ ^(b) ), the absorption coefficient of blood as the standard sample 123 with respect to the light from the first light source 104b is α ₁ ^(w) + M′α ₁ ^(b) , and the light to be irradiated from the second light source 107a The absorption coefficient of the specimen 2 is α ₁ ^(w) + Mα ₂ ^(g) + M′α ₂ ^(b) , and the absorption coefficient of blood as the standard sample 123 with respect to the light from the second light source 107b is α ₁ ^(w) + M _n. If two wavelengths λ ₁ and λ ₂ that are α ₂ ^(g) + M′α ₂ ^(b) (M _n is a known concentration) are selected, the sound wave s ₁₁ for λ ₁ and the sound wave s for sound waves s ₂₁ , λ _{2 12} and the sound wave s ₂₂ can be expressed by Equation (7), respectively.

Therefore, the difference in sound waves between wavelengths for the subject 2 (s ₁₂ −s ₁₁ ) and the difference in sound waves between wavelengths for the blood as the standard sample 123 (s ₂₂ −s ₂₁ ) are reversed in phase. And further subtracting, M ′, α ₁ ^(b) , α ₂ ^(b) , α ₁ ^(w) , α ₂ under the wavelength condition of α ₁ ^(w) = α ₂ ^{(w). (W)} can be deleted, and can be expressed as Equation (8).

Therefore, to detect the acoustic waves s ₁₁ generated from the subject 2 by only the light from the separate first light source 104a, by dividing equation (8), can clear the C in the formula (8), and equation (9) I can express.

Here, compared with α ₁ ^(w) , M′α ₁ ^(b) , which is a component other than the component to be measured, can be ignored and is considered not to affect the calculation accuracy. Therefore, the component concentration M can be calculated by the following formula (10). Here, in the second embodiment described above, the sound waves of different wavelengths recorded in advance on the blood as the standard sample 123 from the difference (s ₁₂ −s ₁₁ ) of sound waves of different wavelengths with respect to the subject. The component concentration M can be similarly calculated by subtracting the difference (s ₂₂ −s ₂₁ ).

As another form, the second sound wave detection procedure may be performed using a biological equivalent material as the standard sample 123 instead of the blood as the standard sample 123 in FIG. In this case, the sound wave s _1t generated from the biological equivalent material as the standard sample 123 by the light from the first light source 104b, and the sound wave s _2t generated from the biological equivalent material as the standard sample 123 by the light from the second light source 107b. , sonic _{s 2h} generated from the subject 2 by the light from the wave _{s 1h} and the second light source 107a generated from the subject 2 by the light from the first light source 104a is expressed respectively equation (11).

According to Expression (11), α ₁ ^(w) (t) cannot be eliminated because the temperature dependence of the water absorbance differs between the two wavelengths. In this case, with respect to the temperature, the difference between α ₁ ^(w) (t) and α ₂ ^(w) (t) increases, causing a problem in the accuracy of calculating the glucose concentration. Therefore, the difference in sound waves between wavelengths for the subject 2 (s _1h −s _2h ) and the difference in sound waves between wavelengths for the bioequivalent material as the standard sample 123 (s _1t −s _2t ), By modulating to the opposite phase and further differentiating, α ₁ ^(w) (t) and α ₂ ^(w) (t) can be eliminated, and can be expressed as Equation (12).

Therefore, by separately detecting the sound wave s _1t generated from the biological equivalent material by only the light from the first light source 104b and dividing the equation (12), C in the equation (12) can be eliminated, and the equation (13) The expressed component concentration M can be calculated.

In Formula (13), the change in α ₁ ^(w) is less than 1% at 1 ° C., and the measurement site does not change by more than 5 degrees by laser heating. The problem is not expected to occur. Here, in the second embodiment described above, the difference between the sound wave of different wavelengths with respect to the subject (s _1h −s _2h ) and the difference between the object to be measured and the biological equivalent material as the standard sample 123. The component concentration M can be similarly calculated by subtracting the difference (s _1t −s _2t ) between the sound waves of the wavelengths.

  When the container 122 shown in FIG. 6 is used instead of the container 121, the blood and the bioequivalent substance as the standard sample 123 in the above two embodiments are arranged at the second focal point 135 and the third focal point 136, respectively. By irradiating the standard samples 151 and 152 with the mixed light from the first light source 104b and the second light source 107b alternately, it is possible to perform correction based on blood and biological equivalent substances without changing the arrangement of the standard samples. Become.

  As described above, in the component concentration measurement method according to the present embodiment, since the sound wave detection unit 108 detects sound waves at the same position, the mixed light from the first light source 104a and the second light source 107a is irradiated. It is possible to simultaneously detect the difference in the magnitude of sound waves generated by the two materials of the subject 2 and the standard sample 123 irradiated with the mixed light from the first light source 104b and the second light source 107b. Therefore, it is possible to correct the change with time of the components of blood and biologically equivalent substances in the subject 2 irradiated with light. It is also possible to correct the influence of components other than the measurement target in the subject 2 irradiated with light, such as blood and biological equivalent substances. Furthermore, since the sound wave detection unit 108 detects the sound wave at the same position, there is no measurement error of sound waves due to individual differences of the sound wave detection unit 108. Therefore, the component concentration measuring apparatus 12 can accurately measure the component concentration of the subject 2 as compared with the case where the two substances are separately measured and corrected after recording.

  Further, the standard sample 123 is blood collected in advance from the subject 2, for example, and after performing the second sound wave detection procedure, the standard sample 123 that is the same as the blood as the standard sample 123 in the second sound wave detection procedure, and the second It is desirable to perform the second sound wave detection procedure once or more for the same subject 2 as the subject 2 in the sound wave detection procedure. Since the component concentration measurement is repeatedly performed on the same subject 2, it is possible to grasp the temporal change for each measurement of the component concentration state of the same subject 2.

  Further, the standard sample 123 is, for example, blood collected in advance from the subject 2, and after the second sound wave detection procedure, a standard sample different from the blood as the standard sample 123 in the second sound wave detection procedure, and in the second sound wave detection procedure It is desirable to perform the second sound wave detection procedure once or more for the same subject 2 as the subject 2. Since component concentration measurement is repeatedly performed on different standard samples, individual differences of the subject 2 can be grasped.

  The component concentration measuring apparatus of the present invention can be used for daily health management and cosmetic check. Moreover, not only humans but also animals can be used for health management. In addition, the component concentration measuring apparatus of the present invention can be applied not only to humans and animals but also to the field of measuring component concentrations in liquids, for example, sugar content measurement of fruits.

It is the figure which showed the light absorbency characteristic of the water and glucose aqueous solution in normal temperature. It is a schematic block diagram of the component concentration measuring apparatus which concerns on 1 embodiment. It is a schematic block diagram of the component concentration measuring apparatus which concerns on 1 embodiment. It is a schematic block diagram of the component concentration measuring apparatus which concerns on 1 embodiment. It is the schematic of the upper surface of the container which concerns on 1 embodiment. It is the top view which showed the other form of the container. It is a figure which shows the structural example of the conventional blood component concentration measuring apparatus by the conventional photoacoustic method. It is a figure which shows the structural example of the conventional blood component concentration measuring apparatus by the conventional photoacoustic method.

Explanation of symbols

2: Subject 10: Component concentration measuring device 11: Component concentration measuring device 12: Component concentration measuring device 101: Oscillator 102: 180 ° phase shift circuit 103: Drive circuit 104: First light source 104a, 104b: First light source 105a, 105b: multiplexer 106: drive circuit 107: second light source 107a, 107b: second light source 108: sound wave detector 109: preamplifier 110: phase detector amplifier 111: component concentration calculator 120: Lid 121: Container 122: Container 123: Standard sample 124: Elliptical spherical surface 125: Elliptical spherical surface 126: Elliptical spherical surface 127: Elliptical spherical surface 128: Elliptical spherical surface 131: First focal point 132: Common focal point 133: Second focal point 134: Common Focus 135: Second focus 136: Third focus 137: First focus 141: Sound wave 142: Sound wave 143: Sound wave 144: Sound wave 145: Sound wave 146 Sound wave 147: Sound wave 151: Standard sample 152: Standard sample 601: First light source 604: Drive circuit 605: Second light source 608: Drive circuit 609: Multiplexer 610: Subject 613: Ultrasonic detector 616: Pulse Light source 617: Chopper plate 618: Motor 619: Acoustic sensor 620: Waveform observer 621: Frequency analyzer

Claims (16)

Light emitting means for emitting two light beams having the same wavelength or different wavelengths from each other by electrically modulating the intensity with signals of the opposite phase at the same frequency; and
A standard sample irradiated with one of the two light beams from the light emitting means;
A sound wave generated from the standard sample by the one light from the light emitting means, and a sound wave generated by the other light from a subject or an object to be irradiated with the other light from the light emitting means, A sound wave detecting means for detecting at the same position with respect to the standard sample and the object or the object to be measured irradiated with the light;
A component concentration measuring device.   The wavelength of the two light beams in the light emitting means so that the magnitude of a sound wave generated from the water becomes zero in a state where the two light beams having different wavelengths are emitted from the light emitting means to the water, respectively. The component concentration measuring apparatus according to claim 1, further comprising wavelength adjusting means for adjusting the wavelength. Measuring light modulation means for electrically intensity-modulating and outputting two waves of the same wavelength with a signal having the same frequency and opposite phase; and
The two light beams of the same wavelength and the two light beams of different wavelengths of the measurement modulation means are electrically intensity-modulated with a signal having an opposite phase at the same frequency as the intensity modulation frequency of the measurement light modulation means Reference light modulation means for outputting;
Combined light emitting means for combining two light beams of opposite phases from the two light beams from the measurement light modulating means and the two light lights from the reference light modulating means to emit two lights. When,
A standard sample irradiated with one of the two lights from the combined light emitting means;
Sound waves generated from the standard sample by the one light from the combined light emitting means, and sound waves generated by the other light from the subject or object to be irradiated with the other light from the combined light emitting means. A sound wave detecting means for detecting the standard sample and the subject irradiated with the light or the object to be measured at the same position;
A component concentration measuring device.   The two waves of the measurement light modulation means and the reference light modulation means so that the magnitude of the sound wave generated from the water becomes zero in a state where the two lights are emitted from the combined light emission means, respectively. 4. The component concentration measuring apparatus according to claim 3, further comprising wavelength adjusting means for adjusting the wavelength of light.   5. The sound wave detection unit according to claim 1, wherein the sound wave detection unit is disposed at a substantially equidistant position from the standard sample and the subject or the measurement object irradiated with the light. The component concentration measuring apparatus as described. An elliptical reflector disposed such that two or more concave elliptical spheres capable of reflecting the sound wave share one of the two focal points of each of the elliptical spheres;
The standard sample is disposed at a position corresponding to another focal point excluding the common focal point of the elliptical sphere,
The sound wave detection means includes the sound wave generated from the subject or the object to be measured that is arranged at the other focus of the elliptical sphere excluding the common focus and the other focus of the elliptical sphere. 6. The component concentration measuring device according to claim 5, wherein the component concentration measuring device is disposed at a position corresponding to the common focal point of the elliptical spherical surface so as to detect sound waves reflected by the elliptical spherical surface.   The standard sample, the object or object to be measured that is irradiated with the light, and the space between the elliptical spherical surface of the elliptical reflector and the sound wave detecting means, and the object or the object that is irradiated with the light The component concentration measuring apparatus according to claim 6, further comprising an acoustic matching material having an acoustic impedance substantially equal to the acoustic impedance of the object to be measured.   8. The component concentration measuring apparatus according to claim 1, wherein the standard sample is water, blood, serum, or a biological equivalent substance.   The light emitting means emits two light beams having the same wavelength or different wavelengths from each other by electrically modulating the intensity with signals having the same frequency and opposite phase, and the sound wave detecting means emits the two waves of light from the light emitting means. The sound wave generated by the one light from the standard sample irradiated with one of the lights, and the object or the object to be measured irradiated with the other of the two waves of light from the light emitting means. A component concentration measurement method comprising a first sound wave detection procedure for detecting a sound wave generated by light at the same position with respect to the standard sample and the subject or the object to be irradiated with the light.   The light emitting means adjusts the wavelength of the two light beams in the light emitting means by electrically modulating the intensity of two light beams having different wavelengths with each other by using signals of the same frequency and opposite phase, and emitting them to water. The wavelength adjustment unit further includes a wavelength adjustment procedure for adjusting the wavelengths of the two light beams so that the magnitude of the sound wave generated from the water becomes zero before the first sound wave detection procedure. Item 9. The component concentration measurement method according to Item 9.   After the first sound wave detection procedure, the same standard sample as the standard sample in the first sound wave detection procedure, and the same object or object to be measured as the subject or the object to be measured in the first sound wave detection procedure The component concentration measurement method according to claim 9, wherein the first sound wave detection procedure is performed once or more.   After the first sound wave detection procedure, a standard sample different from the standard sample in the first sound wave detection procedure, and the same object or the object to be measured in the first sound wave detection procedure The component concentration measurement method according to claim 9, wherein the first sound wave detection procedure is performed once or more.   The measurement light modulation means electrically modulates the intensity of two waves of the same wavelength with a signal having the same frequency and an opposite phase, and the reference light modulation means outputs the two waves of the same wavelength of the measurement modulation means. Two light beams of different wavelengths are electrically intensity-modulated with a signal having an opposite phase at the same frequency as the intensity-modulating frequency of the measurement light modulation means, and a combined light emitting means is output from the measurement light modulation means. Of the two waves of light and the two waves of light from the reference light modulation means are combined to emit two lights having opposite phases, and a sound wave detection means is emitted from the combined light emission means. Sound wave generated from a standard sample irradiated with one of the two lights, and sound wave generated from a subject or object to be irradiated with the other light of the two lights from the combined light emitting means The standard sample and the subject irradiated with the light Component concentration measuring method include a second acoustic wave detection procedure for detecting at the same position relative to the object to be measured.   The measurement light modulation means electrically modulates the intensity of two waves of the same wavelength with a signal having the same frequency and opposite phase, and the reference light modulation means outputs the two waves of the same wavelength of the measurement modulation means. Two light beams having wavelengths different from the light are electrically intensity-modulated with a signal having the same frequency as the intensity modulation frequency of the measurement light modulation means and an opposite phase signal, and the combined light emitting means outputs the measurement light. Of the two light beams from the modulation means and the two light lights from the reference light modulation means, two light beams having opposite phases are combined to emit two lights to the water, respectively, and the measurement light modulation Wavelength adjusting means for adjusting the wavelengths of the two waves of light so that the size of the sound wave generated from the water becomes zero by the wavelength adjusting means for adjusting the wavelengths of the two waves of light in the means and the reference light modulating means, respectively. The procedure is further prepared before the second sound wave detection procedure. Component concentration measuring method according to claim 13, wherein Rukoto.   After the second sound wave detection procedure, the same standard sample as the standard sample in the second sound wave detection procedure, and the same object or object to be measured as the subject or the object to be measured in the second sound wave detection procedure The component concentration measurement method according to claim 13, wherein the second sound wave detection procedure is performed once or more.   After the second wave detection procedure, a standard sample different from the standard sample in the second sound wave detection procedure and the same object or the measurement object as the subject or the measurement object in the second sound wave detection procedure, The component concentration measurement method according to claim 13 or 14, wherein the second sound wave detection procedure is performed once or more.

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