JP2007259821A - Method for producing sugar alcohol - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for effectively using waste beds generated in a large amount and discarded after mushroom cultivation in commercial cultivation of mushrooms. <P>SOLUTION: The method is to produce sugar alcohols included in media after mushroom cultivation by treating the media with 40-100°C hot water, as waste mushroom beds after mushroom cultivation contain a large amount of sugar alcohols. High purity sugar alcohols are produced by treating the hot water-treated product with UF(ultrafiltration) membranes, NF (nanofiltration) membranes or microorganisms. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a method for producing a sugar alcohol from a culture medium after mushroom cultivation.

  In recent years, large-scale fungus bed cultivation has been performed in mushrooms such as shiitake mushrooms, enokitake mushrooms, beech shimeji mushrooms, sea cucumbers, and eringgi, and the production of these mushrooms has been greatly increased. Therefore, the medium used for mushroom fungus cultivation, that is, the waste fungus bed, is produced in large quantities, and some of them are used for compost and livestock bedding, but most of them are discarded. is there. As a method for effectively using the waste fungus bed, it has been studied to reuse the mushroom waste fungus bed in the mushroom medium (for example, Patent Document 1). Further, a method for extracting a mushroom fruit body formation promoting substance from a mushroom waste fungus bed has been studied (for example, Patent Document 2). However, the adjustment method for reusing the mushroom waste bed is complicated, and the method for extracting the mushroom fruiting body formation promoting substance is complicated, which does not lead to effective utilization of the waste bed.

  As sugar alcohols, erythritol, xylitol, sorbitol, mannitol and the like are currently commercially available, and are known to be carbohydrates that exist in nature. Sugar alcohol has lower calories than sucrose, does not cause tooth decay, does not affect blood sugar levels, does not easily cause browning reaction, and is resistant to heat. Food, pharmaceuticals, cosmetics, industrial Widely used in the field. Moreover, although arabitol is not marketed now, it is known that it exists in a fermented seasoning and a mushroom fruiting body, and is known as a saccharide | sugar which is concerned in kokumi. These sugar alcohols are produced by hydrogenating or fermenting glucose or the like as a raw material.

JP-A-6-181630 JP2005-117081

  In this invention, it aims at effective utilization of the waste microbial bed after mushroom cultivation discarded in large quantities.

  The present inventors have found that the waste fungus bed after mushroom cultivation contains a large amount of sugar alcohol, studied the extraction and production method thereof, and completed the present invention.

The present invention according to claim 1 provides a method for producing a sugar alcohol, wherein the sugar alcohol contained in the medium is extracted by treating the medium after mushroom cultivation with hot water at 40 to 100 ° C. To do.
The present invention according to claim 2 allows a sugar alcohol-containing extract obtained by treating a mushroom-cultivated medium with hot water at 40 to 100 ° C. to permeate a UF membrane or NF membrane having a fractional molecular weight of 3000 or less. The present invention provides a method for producing a sugar alcohol characterized by increasing the purity of the sugar alcohol.
This invention which concerns on Claim 3 raises the purity of sugar alcohol by carrying out the microorganism treatment of the sugar alcohol containing extract obtained by processing the culture medium after mushroom cultivation with 40-100 degreeC hot water. A method for producing a sugar alcohol is provided.
The present invention according to claim 4 provides the method for producing a sugar alcohol according to any one of claims 1 to 3, wherein the sugar alcohol is arabitol.

  By producing sugar alcohol from a medium after mushroom cultivation that is discarded in large quantities, that is, a waste fungus bed, the waste fungus bed can be effectively used.

Hereinafter, the present invention will be described in detail.
In the present invention, sugar alcohol can be extracted from the waste fungus bed discharged by mass production of mushrooms. Examples of the sugar alcohol to be obtained include arabitol and D-mannitol, depending on the type of mushroom. D-arabitol is obtained from the enokitake mushroom bed, and D-mannitol is obtained from the mushroom mushroom bed. The enokitake mushroom bed is particularly preferred because it has a high arabitol content and relatively few other coexisting substances.

  The waste bacterial bed used in the present invention can use the medium after harvesting the mushrooms as it is, but the extraction efficiency becomes better when coarsely pulverized so that the medium becomes somewhat fine. In addition, the waste bacteria bed that has passed for a long time after harvesting the mushrooms may be extracted as soon as possible after harvesting the mushrooms because the sugar alcohol content decreases or the bacteria contamination progresses. preferable.

  In order to extract sugar alcohol, it is possible to add a solvent such as water to the waste microbial bed and stir the mixture to easily extract it. Any solvent can be used as long as it dissolves the sugar alcohol, but water is particularly preferable from the viewpoint of ease of handling and price. As the extraction temperature, hot water of about 40 to 100 ° C. may be used. However, when 60 to 100 ° C., sugar alcohol is efficiently extracted, and bacteria in the waste bed are sterilized. Storage becomes easier.

  The solid content concentration of the waste microbial bed at the time of sugar alcohol extraction may be added with a solvent that allows the particles in the waste microbial bed to flow. Specifically, the solid content concentration of the waste microbial bed is 3 to 30 wt. % Or less, preferably 5 to 25% by weight, more preferably 8 to 20% by weight, and sugar alcohol may be extracted. If it exceeds 30% by weight, the fluidity of the medium will be poor, the sugar alcohol will not easily elute into the solvent and will remain inside the waste microbial bed, and solid-liquid separation will be difficult, resulting in poor efficiency. turn into. Further, at a low concentration such as less than 3% by weight, the sugar alcohol-containing liquid becomes dilute, and a lot of energy is required for concentration in the subsequent process. The waste bacterial bed that has been extracted can be separated into a sugar alcohol-containing liquid and a residue by centrifugal separation, compression separation, and various types of filtration.

  The obtained sugar alcohol-containing extract can remove ionic substances and coloring substances by ion exchange resin or activated carbon treatment. In addition, since many polymer components such as polysaccharides coexist in the sugar alcohol-containing extract, the polymer components are allowed to permeate through a UF membrane having a fractional molecular weight of less than 3000 as described in claim 2. It is efficiently removed and the purity of the sugar alcohol can be increased. The UF membrane or NF membrane having a molecular weight cut-off of 3000 or less here may be any membrane that allows permeation of the target sugar alcohol. When the molecular weight cut-off exceeds 3000, impurities such as coloring components contained in the extract will permeate the membrane together with the sugar alcohol. Preferably, the purity of the sugar alcohol can be increased more efficiently by passing through a UF membrane or NF membrane having a molecular weight cut-off of 1000 or less. Furthermore, as described in claim 3, sugars such as glucose that coexist in the sugar alcohol-containing extract are easily assimilated by treatment with microorganisms such as yeast and lactic acid bacteria, and sugar alcohols that are difficult to assimilate It is possible to efficiently increase the purity of. Furthermore, sugar alcohol crystals can be obtained by concentrating and crystallizing a highly purified sugar alcohol-containing liquid. In addition, it can be used as a sugar alcohol-containing liquid with a low purity in applications where there is no problem with a sugar alcohol-containing aqueous solution with a low purity, for example, fertilizers such as plants.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further more concretely, this invention is not limited by these Examples.

  About 60 ° C. hot water was added to the culture medium after enokitake cultivation so that the solid content was 15% by weight, the temperature was adjusted to 60 ° C., and sugar alcohol was extracted while stirring for 30 minutes. After extraction, the residue is separated by centrifuging using a filter cloth, and after removing ionic substances from the resulting sugar alcohol-containing extract using a mixed bed of cation exchange resin and anion exchange resin, HPLC Carbohydrate analysis was performed. As a result of the saccharide analysis, the sugar alcohol-containing extract contained about 0.36% by weight of D-arabitol, and the purity of D-arabitol in the saccharide was 27% as an area percentage.

  The extract obtained by treating the D-arabitol-containing extract obtained in Example 1 with an ion exchange resin was subjected to a test for increasing the purity of D-arabitol using an NF membrane. As the membrane, D-arabitol was concentrated by removing the polymer fraction using a polyester-based NF membrane with a MgSO 4 removal rate of 99%. Although D-arabitol permeates the NF membrane but impurities of the polymer fraction do not permeate the membrane, the purity of D-arabitol was increased in the membrane passage liquid. After the ionic substance was removed from the obtained membrane permeate using a mixed bed of cation exchange resin and anion exchange resin, carbohydrate analysis was performed by HPLC. As a result of the saccharide analysis, the purity of D-arabitol in the passing liquid was 61% in terms of area percentage.

  The D-arabitol-containing extract obtained in Example 1 was tested using baker's yeast to increase the purity of D-arabitol. Commercial baker's yeast was added to the D-arabitol-containing extract and reacted at 30 ° C. for 12 hours. After the reaction, the precipitate is removed by centrifugation, and the resulting supernatant is concentrated under reduced pressure, and then the ionic substance is removed using a mixed bed of a cation exchange resin and an anion exchange resin. Using a cellulose acetate ultrafiltration membrane, the polymer fraction was removed, and D-arabitol was concentrated. Although sugars such as glucose are assimilated by yeast, D-arabitol is not assimilated, so the purity of D-arabitol is increased. After removing ionic substances from the obtained membrane permeate using an ion exchange resin, carbohydrate analysis was performed by HPLC. As a result of carbohydrate analysis, the purity of D-arabitol in the permeate was 80% in area percentage.

The present invention provides a method for producing sugar alcohol from a waste fungus bed by effectively utilizing the waste fungus bed after mushroom cultivation generated by mass production of mushrooms.

Claims (4)

  A method for producing a sugar alcohol, comprising extracting a sugar alcohol contained in a medium by treating the medium after mushroom cultivation with hot water at 40 to 100 ° C.   The sugar alcohol-containing extract obtained by treating the culture medium after mushroom cultivation with hot water at 40 to 100 ° C. permeates the UF membrane or NF membrane having a fractional molecular weight of 3000 or less to increase the purity of the sugar alcohol. Process for producing sugar alcohol characterized   A method for producing a sugar alcohol, wherein the sugar alcohol-containing extract obtained by treating a culture medium after mushroom cultivation with hot water at 40 to 100 ° C. is treated with microorganisms to increase the purity of the sugar alcohol The method for producing a sugar alcohol according to any one of claims 1 to 3, wherein the sugar alcohol is arabitol.