JP2007189951A - Black-rice fermented food - Google Patents

Black-rice fermented food Download PDF

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JP2007189951A
JP2007189951A JP2006011380A JP2006011380A JP2007189951A JP 2007189951 A JP2007189951 A JP 2007189951A JP 2006011380 A JP2006011380 A JP 2006011380A JP 2006011380 A JP2006011380 A JP 2006011380A JP 2007189951 A JP2007189951 A JP 2007189951A
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black rice
rice
fermented
black
fermented food
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Kinji Ishida
均司 石田
Atsuhisa Inaba
敦央 稲葉
Mayumi Arimura
真由美 有村
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Inaba Shokuhin Co Ltd
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Inaba Shokuhin Co Ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide black-rice fermented food having excellent improving effect on decrease in function such as skin function or digestive-system function caused by stress. <P>SOLUTION: The black-rice fermented food is obtained by adding malted rice to cooked black rice and fermenting the mixture. The black-rice fermented food is made into a rice gruel state or a liquid state so as to further improve reduction in regeneration power of skin or in a digestive-system function caused by stress. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、黒米を含有する食品に関し、更に詳しくは、ストレスによる皮膚機能や消化器系の機能における機能低下などを改善する黒米発酵食品に関する。   The present invention relates to a food containing black rice, and more particularly, to a fermented black rice food that improves deterioration of skin function and digestive system function due to stress.

現代はストレス社会ともいわれ、生活環境や職場環境の多様化、人間関係の複雑化により、家庭、仕事、対人関係等にストレスを感じることが多くなっている。人は、このような外部環境からの刺激(ストレス)に対して、神経・内分泌・免疫系を介して体の恒常性を維持しているが、そのストレスにより肉体的、精神的健康を害することがある。   Today, it is also called a stress society. Due to the diversification of living and work environments and the complexity of human relationships, people are often stressed at home, work and interpersonal relationships. Humans maintain their homeostasis through such nerves, endocrine, and immune systems against such external stimuli (stresses), but physical stress and mental health are harmed by such stress. There is.

過度な刺激や過剰な刺激を生体が受けると、交感神経系や内分泌系がその刺激(ストレス)に反応して、副腎皮質ホルモンのみならず、その他多くのホルモンのバランス、自律神経のバランスを崩し、その結果、胃潰瘍、感染症の発症、皮膚機能や免疫能の低下、環境機能の亢進などを来す。その一例に、過密下で飼育され、ストレスを受けている動物の摂餌量、免疫能が低下することが知られている。   When a living body receives excessive or excessive stimulation, the sympathetic nervous system and endocrine system respond to the stimulation (stress) and disrupt not only the adrenal cortex hormones but also many other hormones and autonomic nerves. As a result, gastric ulcer, onset of infection, skin function and immunity decrease, and environmental function increase. As an example, it is known that the amount of food intake and immunity of animals raised under overcrowding and under stress are reduced.

感染症とは、細菌側の病原性と宿主側の防衛力等が複雑に絡み合って病気として発現し様々な病態を呈する疾患であるが、人を含めた動物の感染防御力は、その免疫能や皮膚と粘膜の状態に大きく依存している。   Infectious diseases are diseases in which the pathogenicity on the bacteria side and the defense power on the host side are complicatedly intertwined and manifest as diseases and exhibit various pathological conditions. It depends heavily on the condition of the skin and mucous membranes.

特に、消化器系の不調、例えば、消化不良や便秘、腹部痛等はストレスや食生活に関係しているといわれ、胃潰瘍や十二指腸潰瘍は、ストレスや暴飲暴食による胃酸過多や胃液不足が原因で胃腸の粘膜がただれた状態になる。ストレスの胃粘膜に及ぼす影響は、よく研究されているが、消化器系、粘膜免疫系の最大の器官である腸管に関しては、精神ストレスが及ぼす影響がほとんど分かっていない。   In particular, gastrointestinal problems such as indigestion, constipation, and abdominal pain are said to be related to stress and eating habits. The gastrointestinal mucosa is in a state of sagging. The effects of stress on the gastric mucosa have been well studied, but little is known about the effects of mental stress on the intestinal tract, the largest organ of the digestive system and mucosal immune system.

一方、黒米は、古代米とも言われ、古代から作られ食べられている米である。黒米は、玄米の糖の部分にアントシアニン系の黒色の色素を含む黒い米であり、滋養強壮の作用があり、虚弱体質の改善に効果があるとされ、更に胃腸を丈夫にしたり(保護し、その働きを助けたり)、造血作用もあると言われている。   On the other hand, black rice is also said to be ancient rice, and it has been eaten since ancient times. Black rice is black rice that contains anthocyanin-based black pigment in the sugar part of brown rice, has a tonic action, is said to be effective in improving frail constitution, further strengthens the gastrointestinal (protects, It is said that it also has a hematopoietic effect.

近年、この黒米の効果が見直され、白米に混ぜて炊く以外に、粉末化して麺やパンの生地に混ぜたりして利用されている。その他黒米を含む食品として、粉末化した黒米を粥に含有させる即席黒粥粉末が提案されている(例えば、特許文献1参照)。
特開平8−298947号公報
In recent years, the effect of this black rice has been reviewed, and in addition to cooking by mixing with white rice, it has been used as a powder and mixed with noodles or bread dough. As other foods containing black rice, instant black rice bran powder containing pulverized black rice in rice cake has been proposed (for example, see Patent Document 1).
JP-A-8-298947

しかしながら、ストレスによる皮膚再生力の低下や消化器系の機能低下への黒米の作用は未だに議論されていない。
そこで、本発明は、ストレスによる皮膚再生や消化器系の機能低下について、食品による改善手段を提供することを課題とする。
However, the effect of black rice on the reduction of skin regenerative power and digestive system function due to stress has not been discussed yet.
Then, this invention makes it a subject to provide the improvement means by foodstuffs about the skin reproduction | regeneration and digestive system function fall by stress.

本発明者らは、このような課題を解決すべく鋭意研究を重ねた結果、炊飯された黒米を発酵させた食品とすることでストレスによる皮膚再生力の機能低下や消化器系の機能低下を改善できることを見出し、本発明を完成するに至った。
即ち、本発明は、炊飯された黒米に米こうじを加えて発酵させたことを特徴とする黒米発酵食品に関する。本発明において、黒米は粥状にして発酵させることが好ましい。また、発酵時間は2乃至3時間であることが好ましい。
更に本発明は、炊飯された黒米を発酵させ、この発酵させた黒米に水を加えて攪拌することにより液状としたことを特徴とする黒米発酵食品に関する。この場合、発酵時間は12時間程度であることが好ましい。
As a result of earnest research to solve such problems, the present inventors have reduced the function of skin regeneration and the function of the digestive system due to stress by making fermented food from cooked black rice. The inventors have found that it can be improved and have completed the present invention.
That is, the present invention relates to a fermented black rice food characterized by adding rice koji to fermented black rice and fermenting it. In the present invention, the black rice is preferably fermented in a rice cake shape. The fermentation time is preferably 2 to 3 hours.
Furthermore, this invention relates to the fermented black rice food characterized by fermenting the cooked black rice and making it liquid by adding water and stirring this fermented black rice. In this case, the fermentation time is preferably about 12 hours.

本発明の黒米発酵食品は、ストレスによる消化器系の機能低下などを改善する効果を有しており、この黒米発酵食品を経口投与することにより皮膚再生能力の改善効果もある。   The fermented black rice food of the present invention has an effect of improving the functional degradation of the digestive system due to stress, and has the effect of improving the skin regeneration ability by orally administering the fermented black rice food.

以下、本発明に係る黒米発酵食品について詳細に説明する。
本発明の黒米発酵食品は、黒米とこうじからなり、炊飯された黒米にこうじを加え発酵させることにより、黒米を糖化させることを特徴とする。
Hereinafter, the black rice fermented food according to the present invention will be described in detail.
The black rice fermented food of the present invention comprises black rice and koji, and is characterized by saccharifying black rice by adding koji to the cooked black rice and fermenting it.

黒米は、前述した如く玄米の糖の部分にアントシアニン系の黒色の色素を含む黒い米である。アントシアニンは抗酸化物質として知られるポリフェノールの一種で、血管を保護して動脈硬化を予防する働きや、老化や発ガンの抑制に関係する抗酸化作用がある。その他にも黒米は、ビタミンB1、ビタミンB2、ナイアシン、ビタミンE、鉄、カルシウム、マグネシウム、亜鉛などバランスの取れた栄養成分が含まれている。
従って、黒米には滋養強壮の作用があり、虚弱体質の改善に効果があるとされ、更に胃腸を丈夫にしたり(保護し、その働きを助けたり)、造血作用もあると言われている。
Black rice is black rice containing anthocyanin-based black pigment in the sugar part of brown rice as described above. Anthocyanins are a type of polyphenol known as an antioxidant, and have an action to protect blood vessels and prevent arteriosclerosis and an antioxidant action related to suppression of aging and carcinogenesis. In addition, black rice contains balanced nutritional components such as vitamin B1, vitamin B2, niacin, vitamin E, iron, calcium, magnesium, and zinc.
Therefore, black rice has nourishing and tonic effects, and is said to be effective in improving frail constitutions. It is also said that it has a strong gastrointestinal tract (protects and helps its function) and has a hematopoietic effect.

こうじは、米、麦、大豆などを蒸してこうじ菌を繁殖させたものであり、本発明において、黒米の発酵の主因を為す。本発明の黒米発酵食品では、これらこうじを単独又は組み合わせて用いることができるが、黒米を発酵させるという点から、同種の米からつくられた米こうじを用いることが好ましい。   Koji is obtained by breeding Koji fungi by steaming rice, wheat, soybean, etc., and in the present invention, Koji is the main cause of black rice fermentation. In the black rice fermented food of the present invention, these koji can be used alone or in combination. From the viewpoint of fermenting black rice, it is preferable to use rice koji made from the same kind of rice.

黒米に米こうじを添加する温度は65℃以下が好ましい。65℃より高い温度で添加すると、米こうじの酵素が失活して好ましくない。黒米を発酵させる温度としては、50〜65℃が好ましい。50℃より低い温度で発酵させると酵素が働きにくく、65℃より高い温度になると酵素が失活してしまうため、好ましくない。
また、黒米に加える米こうじの量は、製造する黒米発酵食品の種類や態様に応じて任意に調製すればよく、例えば、粥状の黒米発酵食品とする場合は、黒米の20〜40重量%、甘酒様の液状とした黒米発酵食品とする場合は、黒米の150〜170重量%の米こうじを加えればよい。
The temperature at which rice koji is added to black rice is preferably 65 ° C. or less. If it is added at a temperature higher than 65 ° C, the enzyme of rice koji is inactivated, which is not preferable. As temperature which ferments black rice, 50-65 degreeC is preferable. If fermentation is performed at a temperature lower than 50 ° C., the enzyme does not work easily, and if the temperature is higher than 65 ° C., the enzyme is deactivated.
Moreover, what is necessary is just to prepare arbitrarily the quantity of the rice koji added to black rice according to the kind and aspect of the black rice fermented food to manufacture, for example, when setting it as a bowl-shaped black rice fermented food, it is 20 to 40 weight% of black rice. In the case of a fermented black rice fermented food like amazake, 150 to 170% by weight of rice koji may be added.

次に、粥状の黒米発酵食品を例に取り本発明の黒米発酵食品の製造工程を説明する。
まず、黒米を水洗いし、60分程度浸水する。水切り後、8〜9倍容量の水を加えて1時間程度加熱攪拌して粥状の黒米食品とする。その後、55度付近まで冷却し黒米の30重量%の米こうじを加える。55度前後で温度を維持し2〜3時間加熱攪拌して発酵させる。2時間より加熱攪拌の時間が少ないと発酵不足で好ましくなく、3時間を超えて加熱すると発酵が進み過ぎるため、好ましくない。
その後80度まで加熱して米こうじの発酵を停止させ、粥状の黒米発酵食品とする。
Next, the process for producing the fermented black rice food of the present invention will be described by taking a fermented black rice fermented food as an example.
First, the black rice is washed with water and immersed for about 60 minutes. After draining, add 8 to 9 times the volume of water and heat and stir for about 1 hour to make a rice cake-like black rice food. Then, cool to around 55 degrees and add 30% by weight rice koji of black rice. The temperature is maintained at around 55 degrees, and the mixture is fermented by heating and stirring for 2 to 3 hours. If the time for heating and stirring is less than 2 hours, it is not preferable due to insufficient fermentation, and if it is heated for more than 3 hours, the fermentation proceeds excessively, which is not preferable.
After that, it is heated to 80 degrees to stop the fermentation of rice koji, and a fermented black rice fermented food is obtained.

その他、液状の黒米発酵食品とする場合の製造方法は、黒米を4〜5倍容量の水で炊飯した後、50度付近まで冷却し黒米に対して約160重量%の米こうじを加え、50℃の温度を維持した状態で12時間程度静置し発酵させる。その後、約1/3量の水を加え、ミキサー等で混合して液状の黒米発酵食品とする。   In addition, the manufacturing method in the case of using liquid black rice fermented food is as follows. After cooking black rice with 4-5 times the volume of water, cool it to around 50 degrees and add about 160% by weight rice koji to black rice. The mixture is allowed to stand for about 12 hours and fermented while maintaining the temperature of ° C. Then, about 1/3 amount of water is added and mixed with a mixer or the like to obtain a liquid black rice fermented food.

このように炊飯した黒米を発酵により糖化させた黒米発酵食品には高い抗ストレス作用があり、これを経口投与することにより、ストレスによる皮膚再生力低下及び免疫機能低下の改善効果が期待できる。   The black rice fermented food obtained by saccharifying the cooked black rice by fermentation as described above has a high anti-stress action, and by administering it orally, it can be expected to improve the skin regeneration ability and immune function due to stress.

なお、本発明の黒米発酵食品は、所望に応じて他の成分を含むことができ、例えば、溶液を均一に保つために澱粉を加えることができる。   In addition, the black rice fermented food of this invention can contain another component as desired, for example, starch can be added in order to keep a solution uniform.

本発明の黒米発酵食品は、加圧加熱殺菌(レトルト殺菌)により殺菌処理することもでき、その場合でも抗ストレス作用が見られる。   The black rice fermented food of the present invention can be sterilized by pressure heat sterilization (retort sterilization), and even in that case, an anti-stress effect is observed.

以下、本発明の黒米発酵食品を実施例に基づき説明するが、本発明はこの実施例になんら限定されるものではない。   Hereinafter, although the black rice fermented food of this invention is demonstrated based on an Example, this invention is not limited to this Example at all.

(試験例1:黒米の皮膚再生能力と免疫力における効果)
以下に示す方法に従って、黒米のストレスによる皮膚再生力の低下と消化器系の機能低下の改善作用を調べた。
<被検動物>
HOS:HR−1ヘアレスマウス(12週齢、24‐26g、雌)
<試験群>
普通飼育群:普通飼育(5匹/ゲージ)、普通飼料摂取
対照群 :過密環境飼育(15匹/ゲージ)、普通飼料摂取
黒米摂取群:過密環境飼育(15匹/ゲージ)、黒米混合飼料(3%)摂取
(Test Example 1: Effect of black rice on skin regeneration ability and immunity)
According to the method described below, the effect of improving the reduction of skin regeneration and digestive function due to black rice stress was investigated.
<Test animal>
HOS: HR-1 hairless mouse (12 weeks old, 24-26 g, female)
<Test group>
Normal breeding group: Normal breeding (5 animals / gauge), Normal feed intake control group: Overcrowded environment breeding (15 animals / gauge), Normal feed fed black rice intake group: Overcrowded environment breeding (15 animals / gauge), Black rice mixed feed ( 3%) intake

<黒米混合飼料の作製>
黒米225g(1合)を水洗いし、60分浸水した。水切り後、2倍容量の水(360ml)を加えて炊飯、10分間の蒸らし、放冷後、凍結乾燥した。その後、粉末化し、普通飼育飼料に3重量%の割合で混合し黒米混合飼料を作製した。
<Production of black rice mixed feed>
225 g (one go) of black rice was washed with water and immersed in water for 60 minutes. After draining, 2 volumes of water (360 ml) was added, cooked rice, steamed for 10 minutes, allowed to cool and then lyophilized. Thereafter, the mixture was pulverized and mixed at a ratio of 3% by weight with normal breeding feed to prepare a black rice mixed feed.

上記普通飼育群、対照群及び黒米摂取群の被検動物へアレスマウスをそれぞれケージ(760cm2)内で飼育した。餌は普通飼育群及び対照群には普通飼料を、黒米摂取群には上記作製した黒米混合飼料を自由に摂取させた。6日目に、それぞれ背部の血流量をレーザードップラー計(商品名「PIMII」,Lisca社製)で測定し、更に6日目の糞からIgA ELISA Kit(商品名「Mouse IgA ELISA Quantitation Kit」,BETHYL社製)を用いて免疫IgA量を測定した。結果を表1に示す。
続いて、7日目に各マウスにテープストリッピング(皮膚にテープを貼り、剥がす行為)を10回行い背部の一部に障害を与えた後、その部位の経表皮水分蒸散量(回復率)をその後4日間連続で皮膚水分蒸散量測定装置(商品名「Tewameter TM 210」,Courage+Khazaka社製)を用いて測定した。結果を併せて表1に示す。
Ares mice were reared in cages (760 cm 2) to the test animals of the normal breeding group, the control group, and the black rice intake group. The normal feed group and the control group were allowed to eat the normal feed, and the black rice intake group was allowed to freely receive the prepared black rice mixed feed. On the 6th day, the blood flow in the back was measured with a laser Doppler meter (trade name “PIMII”, manufactured by Lisca), and from the feces on the 6th day, an IgA ELISA Kit (trade name “Mouse IgA ELISA Quantitation Kit”, The amount of immune IgA was measured using BETHYL. The results are shown in Table 1.
Next, on the seventh day, each mouse was subjected to tape stripping (acting and removing the tape on the skin) 10 times to damage a part of the back, and then the transepidermal water transpiration (recovery rate) at that site was measured. Thereafter, the skin moisture transpiration measuring device (trade name “Tewameter TM 210”, manufactured by Courage + Khazaka) was continuously measured for 4 days. The results are also shown in Table 1.

Figure 2007189951
Figure 2007189951

表1からわかるように、対照群に、ストレス的な兆候(摂餌量の低下、体重減少、皮膚再生能(回復率)の低下)が認められた。また、皮膚血流量の低下も起きていた。黒米混合飼料を与えた黒米摂取群は、対照群と比較して摂餌量および体重が増加する傾向を示した。また、皮膚再生能(回復率)も対照群と比較して改善された。更に、免疫IgA量が増加し、免疫力が向上していることがわかった。
上記結果から、黒米にストレスで低下する皮膚再生能力と免疫力に有効な効果が得られることがわかった。
As can be seen from Table 1, stress signs (decreased food consumption, weight loss, decreased skin regeneration ability (recovery rate)) were observed in the control group. In addition, a decrease in skin blood flow occurred. The black rice intake group fed with the black rice mixed diet tended to increase food intake and body weight compared to the control group. In addition, the skin regeneration ability (recovery rate) was also improved compared to the control group. Furthermore, it turned out that the amount of immunity IgA increases and the immunity improves.
From the above results, it was found that black rice has an effective effect on the skin regeneration ability and immunity which are reduced by stress.

(実施例1:黒米発酵食品の皮膚再生能力と免疫力における効果)
本発明の黒米発酵食品のストレスによる皮膚の再生力低下と消化器系の機能低下の改善効果を調べた。
<粥状の黒米発酵食品の作製>
黒米260gを水洗いし、60分間浸水した後、水切りし、水1800gを加え1時間加熱攪拌により炊飯した。
55度まで冷却後、米こうじ80gを加えた。55度前後となるように加温した状態で2時間攪拌し、発酵させた。その後80度まで加熱し、粥状の黒米発酵食品を作製した。
更に、前記粥状の黒米発酵食品250gを加圧加熱殺菌(レトルト殺菌)により、120℃で25分殺菌した殺菌済み粥状黒米発酵食品を作製した。
<液状の黒米発酵食品の作製>
黒米320gを水洗いし、60分浸水した。水切り後、水1200gを加えて炊飯した。炊飯後50度まで冷却し黒米320gに対して米こうじを520g加え、50度で12時間保温状態に保った。その後、680gの水を加え、ミキサーで混合して甘酒様の液状とした黒米発酵食品を作製した。
(Example 1: Effects of fermented black rice food on skin regeneration ability and immunity)
The effects of the fermented black rice food of the present invention on the reduction of the skin's regenerative power and digestive function were investigated.
<Production of fermented black rice fermented food>
260 g of black rice was washed with water, soaked for 60 minutes, drained, 1800 g of water was added, and rice was cooked by heating and stirring for 1 hour.
After cooling to 55 degrees, 80 g of rice koji was added. The mixture was stirred for 2 hours while being heated to about 55 degrees and fermented. Thereafter, the mixture was heated to 80 ° C. to prepare a fermented black rice fermented food.
Furthermore, the sterilized potato-like black rice fermented food which sterilized 25g of the above-mentioned fermented black rice fermented food by pressure heating sterilization (retort sterilization) at 120 ° C. for 25 minutes was prepared.
<Production of liquid black rice fermented food>
320 g of black rice was washed with water and immersed for 60 minutes. After draining, 1200 g of water was added and cooked. After cooking, the mixture was cooled to 50 degrees and 520 g of rice koji was added to 320 g of black rice, and kept at 50 degrees for 12 hours. Thereafter, 680 g of water was added and mixed with a mixer to prepare a fermented black rice food that was made into a liquor-like liquid.

<被検動物>
HOS:HR−1ヘアレスマウス(12週齢、24‐26g、雌)
<試験群>
普通飼育群:普通飼育(5匹/ゲージ)、普通飼料摂取
対照群 :過密環境飼育(15匹/ゲージ)、普通飼料摂取
粥状黒米発酵食品(未殺菌)摂取群:過密環境飼育(15匹/ゲージ)、
粥状黒米発酵食品(未殺菌)混合飼料(3%)摂取
粥状黒米発酵食品(殺菌済)摂取群:過密環境飼育(15匹/ゲージ)、
粥状黒米発酵食品(未殺菌)混合飼料(3%)摂取
液状黒米発酵食品摂取群:過密環境飼育(15匹/ゲージ)、
液状黒米発酵食品混合飼料(3%)摂取
<混合試料の作製>
上記作製した粥状黒米発酵食品、殺菌済みの粥状黒米発酵食品、並びに液状黒米発酵食品を凍結乾燥して粉末化し、普通飼育飼料に3重量%の割合で混合して、粥状黒米発酵食品(未殺菌)混合飼料、粥状黒米発酵食品(未殺菌)混合飼料及び液状黒米発酵食品混合飼料を作製した。
<Test animal>
HOS: HR-1 hairless mouse (12 weeks old, 24-26 g, female)
<Test group>
Normal breeding group: Normal breeding (5 animals / gauge), Normal feed intake control group: Overcrowded environment breeding (15 animals / gauge), Normal feed ingested rod-shaped black rice fermented food (unsterilized) group: Overcrowded environment breeding (15 animals) /gauge),
Fermented black rice fermented food (non-sterilized) mixed feed (3%) Ingested fermented black rice fermented food (sterilized) group: Overcrowded breeding (15 / gauge),
Fermented black rice fermented food (unsterilized) mixed feed (3%) ingested liquid black rice fermented food ingested group: overcrowded breeding (15 / gauge),
Ingestion of liquid black rice fermented food mixed feed (3%) <Preparation of mixed sample>
Fermented black rice fermented food, sterilized fermented black rice fermented food, and liquid black rice fermented food are freeze-dried and pulverized, and mixed at a ratio of 3% by weight with normal rearing feed. (Non-sterilized) mixed feed, rice cake-like black rice fermented food (unsterilized) mixed feed and liquid black rice fermented food mixed feed were prepared.

上記普通飼育群、対照群、粥状黒米発酵食品(未殺菌)摂取群、粥状黒米発酵食品(殺菌済)摂取群、及び液状黒米発酵食品摂取群の被検動物へアレスマウスをそれぞれケージ(760cm2)内で飼育した。餌は、普通飼育群及び対照群には普通飼料を、粥状及び液状黒米発酵食品摂取群には上記作製した混合飼料を自由に摂取させた。
6日目に、それぞれ背部の血流量をレーザードップラー計(商品名「PIMII」,Lisca社製)で測定し、更に6日目の糞からIgA ELISA Kit(商品名「Mouse IgA ELISA Quantitation Kit」,BETHYL社製)を用いて免疫IgA量を測定した。結果を表2に示す。
続いて、7日目に各マウスにテープストリッピング(皮膚にテープを貼り、剥がす行為)を10回行い背部の一部に障害を与えた後、その部位の経表皮水分蒸散量(回復率)をその後4日間連続で皮膚水分蒸散量測定装置(商品名「Tewameter TM 210」,Courage+Khazaka製)を用いて測定した。結果を併せて表2に示す。
The Ares mice were caged to the test animals of the normal breeding group, the control group, the fermented black rice fermented food (unsterilized) ingested group, the fermented black rice fermented food (sterilized) ingested group, and the liquid black rice fermented food ingested group, respectively. 760 cm2). As for the bait, the normal feed group and the control group were allowed to freely feed the normal feed, and the rod-like and liquid black rice fermented food intake groups were allowed to freely feed the prepared mixed feed.
On the 6th day, the blood flow in the back was measured with a laser Doppler meter (trade name “PIMII”, manufactured by Lisca), and from the feces on the 6th day, an IgA ELISA Kit (trade name “Mouse IgA ELISA Quantitation Kit”, The amount of immune IgA was measured using BETHYL. The results are shown in Table 2.
Next, on the seventh day, each mouse was subjected to tape stripping (acting and removing the tape on the skin) 10 times to damage a part of the back, and then the transepidermal water transpiration (recovery rate) at that site was measured. Thereafter, the skin moisture transpiration measuring device (trade name “Tewameter TM 210”, manufactured by Courage + Khazaka) was measured continuously for 4 days. The results are also shown in Table 2.

Figure 2007189951
Figure 2007189951

表2からわかるように、黒米に米こうじを加えた黒米発酵食品は、普通飼育群及び対照群に比べて1日目の皮膚の再生能力が高く、ストレスによって低下する皮膚の再生能力を有意に改善させることがわかった。また、対照群と比べて免疫IgA量が増加しており、ストレスによる皮膚再生と消化器系の機能低下の改善効果があることがわかった。   As can be seen from Table 2, the black rice fermented food with black rice added to the rice koji has higher skin regeneration ability on the first day than the normal breeding group and the control group, and significantly reduces the skin regeneration ability that is reduced by stress. I found it to improve. Moreover, the amount of immunity IgA increased compared with the control group, and it turned out that there exists an improvement effect of the skin reproduction | regeneration by a stress, and the function fall of a digestive system.

(参考例1:黒米発酵食品粉末の抽出物による皮膚再生能力と免疫力における効果)
<試料の調製>
実施例2で作製した粥状黒米発酵食品(未殺菌)を凍結乾燥して得た粉末50.0gを、50%メタノール500mlを用いて溶媒抽出し、得られた抽出物と残渣を減圧濃縮した。その後水をそれぞれ1000ml加え、凍結乾燥した後、粉末化した。
(Reference Example 1: Effect on skin regeneration ability and immunity of black rice fermented food powder extract)
<Preparation of sample>
50.0 g of the powder obtained by freeze-drying the fermented black rice fermented food (non-sterilized) produced in Example 2 was subjected to solvent extraction using 500 ml of 50% methanol, and the obtained extract and the residue were concentrated under reduced pressure. . Thereafter, 1000 ml of water was added, freeze-dried, and powdered.

<被検動物>
HOS:HR−1ヘアレスマウス(12週齢、24‐26g、雌)
<試験群>
普通飼育群:普通飼育(5匹/ゲージ)、普通飼料摂取
対照群 :過密環境飼育(15匹/ゲージ)、普通飼料摂取
粥状黒米発酵食品(抽出物)摂取群:過密環境飼育(15匹/ゲージ)、
粥状黒米発酵食品(抽出物)混合飼料(1.5%)摂取
粥状黒米発酵食品(残渣)摂取群:過密環境飼育(15匹/ゲージ)、
粥状黒米発酵食品(残渣)混合飼料(1.5%)摂取
<混合飼料の作製>
上記作製した粥状黒米発酵食品の抽出物と残渣を粉末化したものを、普通飼育飼料に1.5重量%の割合で混合して、粥状黒米発酵食品(抽出物)混合飼料、及び粥状黒米発酵食品(残渣)混合飼料を作製した。
<Test animal>
HOS: HR-1 hairless mouse (12 weeks old, 24-26 g, female)
<Test group>
Normal breeding group: Normal breeding (5 animals / gauge), Normal feed intake control group: Overcrowded environment breeding (15 animals / gauge), Normal feed ingested potato black rice fermented food (extract) intake group: Overcrowded environment breeding (15 animals) /gauge),
Fermented black rice fermented food (extract) mixed feed (1.5%) ingested Fermented black rice fermented food (residue) ingestion group: Overcrowded breeding (15 animals / gauge),
Ingestion of rice bran fermented food (residue) mixed feed (1.5%) <Preparation of mixed feed>
The above-prepared rice cake-like black rice fermented food extract and residue powdered are mixed with ordinary feed at a ratio of 1.5% by weight, and rice cake-like black rice fermented food (extract) mixed feed, and rice cake Black rice fermented food (residue) mixed feed was prepared.

上記普通飼育群、対照群、粥状黒米発酵食品(抽出物)摂取群及び粥状黒米発酵食品(残渣)摂取群の被検動物に対して実施例1と同様の実験を行った。飼育後6日目の糞より測定した免疫IgA量、及び飼育後7日目に行ったテープストリッピングによるその部位の経表皮水分蒸散量(回復率)をその後4日間連続で測定した結果を表3に示す。   The same experiment as in Example 1 was performed on the test animals of the normal breeding group, the control group, the fermented black rice fermented food (extract) intake group, and the fermented black rice fermented food (residue) intake group. Table 3 shows the results of measurement of the amount of immune IgA measured from feces on the 6th day after breeding and the transepidermal water transpiration (recovery rate) at that site by tape stripping performed on the 7th day after breeding for 4 consecutive days. Shown in

Figure 2007189951
Figure 2007189951

表3の結果より、粥状の黒米発酵食品の抽出物と残渣のどちらにも有意な効果がみられたが、特に残渣に高い回復能がみられた。また、免疫IgA量も対照群と比較してどちらも多く、皮膚再生と免疫機能の回復能力があることがわかった。   From the results in Table 3, significant effects were observed in both the extract and residue of the fermented black rice fermented food, but particularly high recovery ability was observed in the residue. Moreover, the amount of immunity IgA was also large compared with the control group, and it was found that there was a skin regeneration and ability to recover immune function.

Claims (5)

炊飯された黒米に米こうじを加えて発酵させたことを特徴とする黒米発酵食品。 A fermented black rice food characterized by adding rice koji to fermented black rice and fermenting it. 粥状の黒米を発酵させたことを特徴とする請求項1に記載の黒米発酵食品。 The black rice fermented food according to claim 1, wherein rice cake-shaped black rice is fermented. 発酵時間が2乃至3時間であることを特徴とする請求項2に記載の黒米発酵食品。 The fermented black rice food according to claim 2, wherein the fermentation time is 2 to 3 hours. 発酵させた黒米に水を加えて攪拌することにより液状としたことを特徴とする請求項1に記載の黒米発酵食品。 The black rice fermented food according to claim 1, wherein the fermented black rice is made liquid by adding water and stirring. 発酵時間が12時間程度であることを特徴とする請求項4に記載の黒米発酵食品。 The black rice fermented food according to claim 4, wherein the fermentation time is about 12 hours.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014187997A (en) * 2013-03-28 2014-10-06 Origin Biochemical Laboratory Inc Anti-aging food
JP2015057050A (en) * 2013-08-13 2015-03-26 株式会社シャローム Method for producing polyphenol-containing culture and polyphenol-containing culture
CN110881504A (en) * 2019-12-17 2020-03-17 陕西科技大学 Black rice and coarse cereal bread and making method thereof
JP2020112407A (en) * 2019-01-10 2020-07-27 日本メナード化粧品株式会社 Method for evaluating immunity strength

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014187997A (en) * 2013-03-28 2014-10-06 Origin Biochemical Laboratory Inc Anti-aging food
JP2015057050A (en) * 2013-08-13 2015-03-26 株式会社シャローム Method for producing polyphenol-containing culture and polyphenol-containing culture
JP2020112407A (en) * 2019-01-10 2020-07-27 日本メナード化粧品株式会社 Method for evaluating immunity strength
JP7224586B2 (en) 2019-01-10 2023-02-20 日本メナード化粧品株式会社 Immunity evaluation method
CN110881504A (en) * 2019-12-17 2020-03-17 陕西科技大学 Black rice and coarse cereal bread and making method thereof

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