JP2007151436A - Fermented black garlic extract liquid, fermented black garlic powder, method for producing the same, and food and drink containing the same - Google Patents
Fermented black garlic extract liquid, fermented black garlic powder, method for producing the same, and food and drink containing the same Download PDFInfo
- Publication number
- JP2007151436A JP2007151436A JP2005349229A JP2005349229A JP2007151436A JP 2007151436 A JP2007151436 A JP 2007151436A JP 2005349229 A JP2005349229 A JP 2005349229A JP 2005349229 A JP2005349229 A JP 2005349229A JP 2007151436 A JP2007151436 A JP 2007151436A
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- Prior art keywords
- black garlic
- fermented black
- garlic
- fermented
- extract
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Abstract
Description
本発明は、生ニンニクを自己醗酵させた醗酵黒ニンニクの醗酵黒ニンニク抽出物、醗酵黒ニンニクパウダー、これらの製造方法及びこれらを含む飲食品に関する。 TECHNICAL FIELD The present invention relates to a fermented black garlic extract of fermented black garlic obtained by self-fermenting raw garlic, a fermented black garlic powder, a production method thereof, and a food and drink containing these.
ニンニクは、中央アジアが原産地で、学名をアリウム サチバム エル(Allium sativum L)という。食用(薬用)部分は鱗茎であり、有効成分としてアリシンが認められる。
アリシンは元来含まれているアリインが酵素のアリイナーゼの働きにより変化を受けて生じたもので、ニンニク特有の強烈な臭いを放つ物質である。古来、ニンニクはスタミナ増強に強い効果のある食品としてそのまま、あるいは他の食材の調味料として幅広く用いられている。近年、ニンニクの食効についても科学的な検討が加えられ、抗疲労や抗ストレス効果は勿論のこと、高い抗酸化作用に基づく生活習慣病の予防効果、免疫賦活、抗ウイルス、抗菌作用による感染症予防効果、肝臓保護効果、老化防止効果など多様な効果が認められている。特に、アメリカのデザイナーフーズプログラムにおいては、抗ガン効果が最も高い食品素材としてリストアップされるなど、有用食品の代表格といっても過言ではない。しかしこれほど有用なニンニクも先に述べた有効成分アリシンの強い臭いや、特に摂取後に吐息と共に戻ってくる強烈な不快臭のために食材として敬遠される傾向にあった。
The garlic originates from Central Asia and has the scientific name Allium sativum L. The edible (medicinal) part is a bulb and allicin is recognized as an active ingredient.
Allicin is a substance that is produced by the change of alliin originally contained by the action of the enzyme alliinase, and emits an intense smell peculiar to garlic. Since ancient times, garlic has been widely used as it is as a food having a strong effect on enhancing stamina or as a seasoning for other ingredients. In recent years, scientific investigations have also been made on the food effect of garlic, not only anti-fatigue and anti-stress effects, but also preventive effects on lifestyle-related diseases based on high antioxidant effects, immunostimulation, anti-viral and anti-bacterial effects Various effects such as prevention of liver disease, liver protection and anti-aging are recognized. In particular, in the American designer foods program, it is no exaggeration to say that it is a representative of useful foods, such as being listed as the food material with the highest anticancer effect. However, such useful garlic tended to be avoided as a food because of the strong odor of the active ingredient allicin mentioned above and the strong unpleasant odor that comes back with sighing after ingestion.
そのため、従来、このような臭いの問題点の解決を図るために種々の検討がなされている。例えば、、酵母発酵を利用して製造する食後無臭ニンニク(特許文献1参照)、生ニンニクを超高圧で処理し、アリイナーゼを不活性化する無臭ニンニクの製造方法(特許文献2参照)、蒸煮にんにくと練りゴマを混合し、該混合物に黄な粉及び蜂蜜を混合してなるペースト状無臭にんにく加工食品(特許文献3参照)、ニンニクの亜種を原料とするもの等多くの提案があり、これまでに市場には、無臭ニンニクと称する商品が数多く出回るようになり、一般食品や健康食品分野で幅広く採用されてきている。
しかし、従来の多くの無臭ニンニクは、摂取後に呼気から臭ってくるニンニク臭の除去が不十分であったり、無臭化加工によりニンニクの有効性が損なわれてしまったりと、充分に満足できるものが少ないというのが実情であった。また、このような無臭ニンニクなるものを配合した食品についても、同様の問題が示唆されていた。更に、ニンニクの有効成分を効果が期待できる程度に摂取するには、現実的ではない量のニンニクを摂取する必要があり、ニンニクの有用な成分を効率的に得る手段が求められていた。また、飲食物や医薬などに配合し易い態様の醗酵黒ニンニクの加工物が望まれていた。 However, many conventional odorless garlics are sufficiently satisfactory, such as insufficient removal of the garlic odor that smells from breath after ingestion, or the loss of garlic effectiveness due to non-bromide processing. The fact was that there were few. Similar problems have been suggested for foods containing such odorless garlic. Furthermore, in order to ingest an effective ingredient of garlic to such an extent that the effect can be expected, it is necessary to ingest an unrealistic amount of garlic, and means for efficiently obtaining a useful ingredient of garlic has been demanded. Moreover, the processed material of the fermentation black garlic of the aspect which is easy to mix | blend with food-drinks, a medicine, etc. was desired.
本発明は上記の事情に鑑みなされたものであり、摂取後の呼気にニンニク臭が発生しない上、優れた生理活性を効率的に得ることができ、飲食物や医薬に配合し易い加工物である、生ニンニクを自己醗酵させた醗酵黒ニンニクの抽出物及び乾燥・粉砕物並びにこれらの製造方法及びこれらを含む飲食品を提供することを課題とする。 The present invention has been made in view of the above circumstances, and does not generate garlic odor in the breath after ingestion, and can obtain excellent physiological activity efficiently, and is a processed product that can be easily blended in foods and drinks and medicines. It is an object of the present invention to provide a fermented black garlic extract obtained by self-fermenting raw garlic, a dried and pulverized product, a production method thereof, and a food and drink containing these.
本発明は、生ニンニクを自己醗酵させた醗酵黒ニンニクの抽出物である醗酵黒ニンニク抽出液を要旨とする。また、生ニンニクを自己醗酵させた醗酵黒ニンニクの乾燥・粉砕物である醗酵黒ニンニクパウダーを要旨とする。醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーの原料となる醗酵黒ニンニクは、温度が55〜80℃、湿度が70〜95%の範囲内で熟成し自己醗酵させたものが好ましい。 The gist of the present invention is a fermented black garlic extract which is an extract of fermented black garlic obtained by self-fermenting raw garlic. In addition, the gist is fermented black garlic powder, which is a dried and pulverized product of fermented black garlic obtained by self-fermenting raw garlic. The fermented black garlic used as a raw material for the fermented black garlic extract or the fermented black garlic powder is preferably aged and self-fermented within a temperature range of 55 to 80 ° C. and a humidity of 70 to 95%.
本発明は、生ニンニクを自己醗酵させる工程と、得られた醗酵黒ニンニクをペースト状にする工程と、ペースト状の醗酵黒ニンニクを抽出する工程と、抽出物を濃縮する工程と、からなる醗酵黒ニンニク抽出液の製造方法を要旨とする。また、本発明は、生ニンニクを自己醗酵させる工程と、得られた醗酵黒ニンニクを乾燥する工程と、乾燥した醗酵黒ニンニクを粉砕する工程と、からなる醗酵黒ニンニクパウダーの製造方法を要旨とする。 The present invention comprises a step of self-fermenting raw garlic, a step of pasting the obtained fermented black garlic, a step of extracting paste-like fermented black garlic, and a step of concentrating the extract. The gist of the method for producing black garlic extract. The gist of the present invention is a method for producing fermented black garlic powder comprising the steps of self-fermenting raw garlic, the step of drying the obtained fermented black garlic, and the step of grinding the dried fermented black garlic. To do.
本発明は、上記の醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーを含む飲食品、あるいは医薬を要旨とする。 The gist of the present invention is a food or drink containing the above fermented black garlic extract or fermented black garlic powder, or a medicine.
本発明の醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーは、摂取後の呼気にニンニク臭の発生がなく、ニンニク臭を気にすることなく日常的に摂取できる。また、醗酵黒ニンニク抽出液は水溶性で、醗酵黒ニンニクパウダーはパウダー状なので、飲食物や医薬に配合し易く、ニンニクの生理活性のある有用な成分を効率的に摂取できる。 The fermented black garlic extract or fermented black garlic powder of the present invention does not generate garlic odor in the breath after ingestion, and can be ingested on a daily basis without worrying about the garlic odor. Moreover, since the fermented black garlic extract is water-soluble and the fermented black garlic powder is in a powder form, it can be easily incorporated into foods and drinks and medicines, and can effectively ingest useful components having the physiological activity of garlic.
本発明の醗酵黒ニンニク抽出液の製造方法又は醗酵黒ニンニクパウダーの製造方法は、生ニンニクに酵母等の添加物を介在させることなく、所定の温度と湿度の下で熟成させる醗酵黒ニンニクを抽出又は乾燥・粉砕するだけで得られるので、ニンニク臭のしない、ニンニクの有用な成分を簡便かつ経済的に得ることができる。 The method for producing the fermented black garlic extract or the method for producing the fermented black garlic powder of the present invention extracts the fermented black garlic that is aged under a predetermined temperature and humidity without interposing an additive such as yeast in the raw garlic. Alternatively, since it is obtained simply by drying and pulverization, a useful component of garlic without garlic odor can be obtained simply and economically.
本発明の飲食物又は医薬は、ニンニク臭を気にすることなく、ニンニクの有用な成分を日常的に効率的に摂取できる。 The food or drink or medicine of the present invention can efficiently ingest daily useful components of garlic without worrying about the garlic odor.
本発明の醗酵黒ニンニク抽出液は、生ニンニクを自己醗酵させて醗酵黒ニンニクを得る第一工程と、ペースト状にする第二工程と、水及び親水性有機溶媒を用いて抽出する第三工程と、遠心分離して濃縮する第四工程により製造できる。使用する生ニンニクは、特に限定がなく、例えば、国内産、中国産など産地も限定されない。 The fermented black garlic extract of the present invention is a first step for self-fermenting raw garlic to obtain fermented black garlic, a second step for making a paste, and a third step for extraction using water and a hydrophilic organic solvent. And the fourth step of concentrating by centrifugation. The raw garlic to be used is not particularly limited, and the production area is not limited, for example, domestic production or Chinese production.
第一工程である自己醗酵は、温度が55〜80℃、好ましくは60〜75℃、より好ましくは65〜70℃の範囲内で、湿度が70〜95%、好ましくは75〜90%、より好ましくは80〜85%の範囲内で熟成させて行うことができる。生ニンニクは、温度が55℃より低く、湿度が70〜95%の範囲外になると、自己醗酵ではなく腐敗が進み、形が崩れて泥状になり、食用できない。また、温度が80℃より高く、湿度が70〜95%の範囲外となると、ニンニクは単なる乾燥物となり、淡褐色の硬い石状になってしまい、食品として不向きとなる。また、温度が55〜80℃、湿度が70〜95%の範囲外となると、ニンニク中に含まれる悪臭の原因物質の油溶性のアリルメチルスルフィドが水溶性のS‐アリルシステインに変化せず、摂取後のニンニクの臭いを除去できない。 Self-fermentation, which is the first step, has a temperature of 55 to 80 ° C, preferably 60 to 75 ° C, more preferably 65 to 70 ° C, and a humidity of 70 to 95%, preferably 75 to 90%. It can be carried out by aging preferably in the range of 80 to 85%. When raw garlic is lower than 55 ° C and humidity is outside the range of 70 to 95%, it does not undergo self-fermentation, but decays and becomes mud and cannot be edible. On the other hand, when the temperature is higher than 80 ° C. and the humidity is out of the range of 70 to 95%, the garlic becomes a mere dry substance, becomes a light brown hard stone, and is unsuitable as a food. In addition, when the temperature is outside the range of 55 to 80 ° C. and the humidity is 70 to 95%, the oil-soluble allyl methyl sulfide, which is the causative substance of malodor contained in garlic, does not change to water-soluble S-allyl cysteine, Can't remove garlic odor after ingestion.
生ニンニクを醗酵・熟成させる期間は、温度と湿度が上記の雰囲気下で熟成中のニンニクの状態を観察して適宜設定できるが、通常8〜30日間とできる。 The period for fermenting and ripening raw garlic can be appropriately set by observing the state of garlic being ripened under the above atmosphere at the temperature and humidity, but it can usually be 8-30 days.
このように、ニンニクの熟成の温度と湿度を管理することにより、自己醗酵を進行させられる結果、得られる醗酵黒ニンニクは摂取後の呼気にニンニク臭を発生させない上、抗酸化活性、ポリフェノール含量、等のニンニクの生理活性が高められる。 Thus, by controlling the temperature and humidity of aging of garlic, as a result of proceeding self-fermentation, the resulting fermented black garlic does not generate garlic odor in the breath after ingestion, and also has antioxidant activity, polyphenol content, The physiological activity of garlic such as is increased.
第二工程であるペースト化は、醗酵黒ニンニクの皮を除去し、潰してペースト状にする。ペースト化は、乳鉢、ミンチ機、ミキサー等公知の手段を用いて行うことができる。 The second step, pasting, removes the peel of fermented black garlic and crushes it into a paste. Pasting can be performed using a known means such as a mortar, a mincing machine, a mixer.
第三工程である抽出は、ペーストに温度が40〜80℃、好ましくは40〜75℃、より好ましくは45〜70℃の水を加え、撹拌する。撹拌は、ブレンダー、ミキサー等の公知の手段を用いて行うことができる。撹拌抽出後、冷却水を用いて冷却し、親水性有機溶媒を添加し混合する。抽出溶媒として使用し得る水としては、純水、水道水、井戸水、鉱泉水、鉱水、温泉水、湧水、淡水等の他、これらに各種処理を施したものが含まれる。水に施す処理としては、例えば、精製、加熱、殺菌、濾過、イオン交換、浸透圧の調整、緩衝化等が含まれる。従って、本発明において抽出溶媒として使用し得る水には、精製水、熱水、イオン交換水、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水等も含まれる。 In the extraction, which is the third step, water having a temperature of 40 to 80 ° C., preferably 40 to 75 ° C., more preferably 45 to 70 ° C. is added to the paste and stirred. Stirring can be performed using a known means such as a blender or a mixer. After stirring and extracting, the mixture is cooled with cooling water, and a hydrophilic organic solvent is added and mixed. Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.
抽出溶媒として使用し得る親水性有機溶媒としては、例えば、メタノール、エタノール、プロピルアルコール、イソプロピルアルコール、ブタノール等の炭素数1〜4の低級脂肪族アルコール、1,3‐ブチレングリコール、プロピレングリコール、グリセリン等の多価アルコール、アセトン、メチルエチルケトン等の低級脂肪族ケトン又はこれらの混合物等が挙げられる。抽出溶媒量は、抽出原料の1〜10倍量、好ましくは3〜5倍量である。 Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, isopropyl alcohol, and butanol, 1,3-butylene glycol, propylene glycol, and glycerin. And polyhydric alcohols such as acetone, lower aliphatic ketones such as acetone and methyl ethyl ketone, and mixtures thereof. The amount of extraction solvent is 1 to 10 times, preferably 3 to 5 times the amount of the extraction raw material.
次いで、遠心分離により得られた抽出液の不溶物を除去し、減圧濃縮により乾燥残分60〜65%まで濃縮する第四工程により水溶性のある醗酵黒ニンニク抽出液が得られる。 Subsequently, the insoluble matter of the extract obtained by centrifugation is removed, and a water-soluble fermented black garlic extract is obtained by the fourth step of concentrating to a dry residue of 60 to 65% by vacuum concentration.
また、本発明の醗酵黒ニンニクパウダーは、生ニンニクを自己醗酵させて醗酵黒ニンニクを得る第一工程と、これを乾燥して粉砕する第二工程より製造できる。原料となる醗酵黒ニンニクは、既述の醗酵黒ニンニク抽出液の原料となる醗酵黒ニンニクと全く同様に製造できるので、重複する記載は割愛する。 Moreover, the fermented black garlic powder of this invention can be manufactured from the 1st process of making raw garlic self-ferment and obtaining fermented black garlic, and the 2nd process of drying and grind | pulverizing this. Since the fermented black garlic used as a raw material can be manufactured in exactly the same manner as the fermented black garlic used as the raw material for the fermented black garlic extract described above, duplicate descriptions are omitted.
醗酵黒ニンニクの乾燥は、温度55〜80℃、好ましくは60〜75℃、より好ましくは70〜75℃の範囲内で熱風乾燥させて行うことができる。醗酵黒ニンニクを乾燥させる期間は、温度が上記の雰囲気下で醗酵黒ニンニクの状態を観察して適宜設定できるが、通常25〜35日間とできる。粉砕は吸湿に注意し、エアコンの条件下等で行う。また、粉砕はピンミル等の公知の手段を用いて行うことができる。粉砕後の醗酵黒ニンニクは50〜100メッシュ、好ましくは60メッシュのパンチングスクリーンにより篩過処理を行う。 The dried fermented black garlic can be dried by hot air drying at a temperature of 55-80 ° C, preferably 60-75 ° C, more preferably 70-75 ° C. The period for drying the fermented black garlic can be appropriately set by observing the state of the fermented black garlic under the above-mentioned atmosphere, but can usually be 25 to 35 days. Grinding should be done under the condition of air conditioner, paying attention to moisture absorption. The pulverization can be performed using a known means such as a pin mill. The fermented black garlic after pulverization is sieved by a punching screen of 50 to 100 mesh, preferably 60 mesh.
上記で得られた醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーは、任意の飲食品に含ませることができ、また、医薬に含ませることもできる。醗酵黒ニンニク抽出液を配合し得る飲食品は特に限定されず、また、飲食品には健康食品も含まれる。飲食品の具体例としては、清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等の飲料(これらの飲料の濃縮原液及び調整用粉末を含む)、アイスクリーム、アイスシャーベット、かき氷等の冷菓、そば、うどん、はるさめ、ギョウザの皮、シュウマイの皮、中華麺、即席麺等の麺類、飴、チューインガム、キャンディー、ガム、チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子等の菓子類、かまぼこ、ハム、ソーセージ等の水産・畜産加工食品、加工乳、発酵乳等の乳製品、サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等の油脂及び油脂加工食品、ソース、タレ等の調味料、スープ、シチュー、サラダ、惣菜、漬物、パン等が挙げられる。 The fermented black garlic extract or fermented black garlic powder obtained above can be included in any food or drink, and can also be included in medicine. The food and drink which can mix | blend a fermented black garlic extract is not specifically limited, Moreover, health food is also contained in food and drink. Specific examples of food and drink include beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (including concentrated concentrates and powders for adjustment of these drinks), ice cream, ice sherbet, shaved ice, and other frozen desserts. , Soba, udon, harusame, gyoza skin, shumai skin, noodles such as Chinese noodles, instant noodles, rice cake, chewing gum, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, etc. Confectionery, processed fishery products such as kamaboko, ham, sausage, processed milk, dairy products such as fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc. Examples include seasonings such as sauces and sauces, soups, stews, salads, prepared dishes, pickles, and bread.
任意の飲食物における醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーの配合量は、飲食物の形態や嗜好に応じて適宜調整することができるが、好適な配合率は約0.01wt%〜20.0wt%、好ましくは5wt%程度である。また、医薬として用いる場合の配合率は、通常、0.01wt%〜20.0wt%、好ましくは5wt%程度であるが、使用者の性別、体重、年齢、症状等を考慮して適宜増減できる。 The blending amount of the fermented black garlic extract or fermented black garlic powder in any food or drink can be adjusted as appropriate according to the form and taste of the food and drink, but the preferred blending ratio is about 0.01 wt% to 20.0 wt%. Preferably, it is about 5 wt%. In addition, the compounding ratio when used as a medicine is usually 0.01 wt% to 20.0 wt%, preferably about 5 wt%, but can be appropriately increased or decreased in consideration of the gender, weight, age, symptoms, etc. of the user.
以下、本発明を実施例を挙げて説明するが、本発明は以下の実施例に限定されるものではない。 EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited to a following example.
〔実施例1〕(醗酵黒ニンニク抽出液の製造)
中国産生ニンニク100kgを圧搾空気によりエアー洗浄した後、10枚のトレイに分配した。次いで、トレイを醗酵室に入れ、醗酵室の温度を65℃、湿度を85%に設定し、30日間放置し自己醗酵させた。得られた醗酵黒ニンニクの鱗茎は真っ黒に変化していた。
[Example 1] (Production of fermented black garlic extract)
100 kg of Chinese garlic was air washed with compressed air and then distributed to 10 trays. Next, the tray was placed in a fermentation room, the temperature of the fermentation room was set to 65 ° C., and the humidity was set to 85%, and left for 30 days for self-fermentation. The resulting fermented black garlic bulbs turned black.
得られた醗酵黒ニンニク50kgの皮をむき、ミンチ機によりペースト状にした。ペーストに温度50℃の水160Lを加え、ブレンダーにより30分間撹拌抽出した。これを冷却水により30℃まで冷却した後、食品添加物用エタノール36Lを添加し混合した。遠心脱水機により不溶物を除去して得られた抽出エキスを、減圧濃縮により乾燥残分60〜65%まで濃縮することにより、醗酵黒ニンニク抽出液を得た。 The obtained fermented black garlic 50 kg was peeled and made into a paste by a mincing machine. 160 L of water having a temperature of 50 ° C. was added to the paste, and the mixture was extracted by stirring for 30 minutes with a blender. After cooling this to 30 ° C. with cooling water, 36 L of food additive ethanol was added and mixed. The extract obtained by removing the insoluble material with a centrifugal dehydrator was concentrated to 60 to 65% of the dry residue by vacuum concentration to obtain a fermented black garlic extract.
〔実施例2〕(醗酵黒ニンニク抽出液のパネルテスト1)
実施例1で得られた醗酵黒ニンニク抽出液を5名のパネラーに試食させ、食べた後18時間後の呼気中のニンニク臭の有無、体調の変化の3項目についてパネルテストを行った。
[Example 2] (Panel test 1 of fermented black garlic extract)
A panel test was conducted on three items of the presence or absence of garlic odor in exhaled breath and change in physical condition 18 hours after eating the fermented black garlic extract obtained in Example 1 for 5 panelists.
表1から明らかなように、醗酵黒ニンニク抽出液はニンニク特有の臭いは有しているものの、生ニンニクのような刺激臭は消失しており、風味も甘さも増し、そのまま食べても胃を損なうことがなかった。また、特筆すべきは、醗酵黒ニンニク抽出液を摂取しても、呼気のニンニク臭さが全くなく、余人にニンニクを食べたことが全く気付かれないことであった。以上のような良好なパネルテストの結果により、醗酵黒ニンニク抽出液の有用性が充分に確認できた。 As is clear from Table 1, the fermented black garlic extract has a garlic-specific odor, but the irritating odor like raw garlic has disappeared. There was no loss. Also noteworthy was that even when the fermented black garlic extract was ingested, there was no garlic odor of exhaled breath, and it was completely unnoticeable to eat garlic. The usefulness of the fermented black garlic extract was fully confirmed by the results of the good panel test as described above.
〔実施例3〕(醗酵黒ニンニク抽出液のパネルテスト2)
醗酵黒ニンニク抽出液5gを清涼飲料水100gに溶解したものを5名のパネラーに試飲させてパネルテストを行った。
[Example 3] (Panel test 2 of fermented black garlic extract)
A panel test was conducted by tasting 5 panelists of 5 g of fermented black garlic extract dissolved in 100 g of soft drink.
醗酵黒ニンニク抽出液は、水溶性であるため清涼飲料水に容易に溶解させることができた。さらに表2より、醗酵黒ニンニク抽出液を清涼飲料水に添加する事により、醗酵黒ニンニク抽出液は摂取しやすくなり、また摂取後の呼気にニンニク臭がないため、抵抗なく摂取できることがパネラーの評価より明らかになった。 Since the fermented black garlic extract was water-soluble, it could be easily dissolved in soft drinks. Furthermore, from Table 2, the addition of fermented black garlic extract to soft drinks makes it easier to ingest fermented black garlic extract, and since there is no garlic odor in the breath after ingestion, it can be consumed without resistance. It became clear from the evaluation.
〔実施例4〕(醗酵黒ニンニクパウダーの製造)
中国産生ニンニク100kgを圧搾空気によりエアー洗浄した後、10枚のトレイに分配した。次いで、トレイを醗酵室に入れ、醗酵室の温度を65℃、湿度を85%に設定し、30日間放置し自己醗酵させた。得られた醗酵黒ニンニクの鱗茎は真っ黒に変化していた。
得られた醗酵黒ニンニクを乾燥室に入れ、乾燥室の温度を75℃に設定し、30日間乾燥させた。乾燥後、粉砕し、60メッシュパスにより篩過処理を施し、醗酵黒ニンニクパウダーを得た。
[Example 4] (Production of fermented black garlic powder)
100 kg of Chinese garlic was air washed with compressed air and then distributed to 10 trays. Next, the tray was placed in a fermentation room, the temperature of the fermentation room was set to 65 ° C., and the humidity was set to 85%, and left for 30 days for self-fermentation. The resulting fermented black garlic bulbs turned black.
The obtained fermented black garlic was put in a drying room, the temperature of the drying room was set to 75 ° C., and dried for 30 days. After drying, it was pulverized and sieved through a 60 mesh pass to obtain fermented black garlic powder.
〔実施例5〕(醗酵黒ニンニクパウダーのパネルテスト)
実施例4で得られた醗酵黒ニンニクパウダーを5名のパネラーに試食させ、食べた後18時間後の吐息中のニンニク臭の有無、体調の変化の3項目についてパネルテストを行った。
[Example 5] (Panel test of fermented black garlic powder)
A panel test was conducted on three items: presence of garlic odor during breathing and change in physical condition 18 hours after eating the fermented black garlic powder obtained in Example 4, which was tasted by five panelists.
表3から明らかなように、醗酵黒ニンニクパウダーはニンニク特有の臭いは有しているものの、生ニンニクのような刺激臭は消失しており、風味も甘さも増し、そのまま食べても胃を損なうことがなかった。また、特筆すべきは、醗酵黒ニンニクパウダーを摂取しても、呼気のニンニク臭さが全くなく、余人にニンニクを食べたことが全く気付かれないことであった。以上のような良好なパネルテストの結果により、醗酵黒ニンニクパウダーの有用性が充分に確認できた。 As is clear from Table 3, fermented black garlic powder has a garlic-specific odor, but the irritating odor like raw garlic has disappeared, flavor and sweetness increase, and even if eaten as it is, the stomach is damaged. It never happened. Also noteworthy was that even when fermented black garlic powder was ingested, there was no garlic odor of exhaled breath, and it was completely unnoticeable to eat garlic. The usefulness of the fermented black garlic powder was fully confirmed by the results of the good panel test as described above.
〔実施例6〕(醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーの抗酸化活性の評価)
醗酵黒ニンニク抽出液、醗酵黒ニンニクパウダー、青森県天間林産生ニンニク、中国産生ニンニクのそれぞれについて、下記のようにDPPH分光測定法によりラジカル消去能を測定し、抗酸化活性を評価した。
[Example 6] (Evaluation of antioxidant activity of fermented black garlic extract and fermented black garlic powder)
The radical scavenging ability of each of fermented black garlic extract, fermented black garlic powder, Aomori Tenmabayashi-produced garlic, and Chinese-produced garlic was measured by DPPH spectroscopy as described below, and the antioxidant activity was evaluated.
400μM DPPH 36 mL、200mM MES緩衝液36 mL、20%エタノール 36 mLの混液を作り、試験管に2.7 mLずつ分注した(40サンプル測定用)。さらに80%エタノールを(900−a)μL加えた。これに分析試料 aμLを30秒おきに加え、ボルテックスで混合した(a=0、90、180、360、540、720μLの順に加えていく)。分析試料添加20分後に520nmにおける吸光度を測定した。添加順に従い、順次測定していく。分析試料を添加しないもの(a=0)の吸光度を100%とし、吸光度50%に相当する分析試料添加量を求め、DPPH50%消失量(μL/3.6mL assay)とした。結果は表4に示した。なお、各分析試料は80%エタノールを用いて調製し、10mg/mL、50mg/mLに調整した。 A mixture of 36 mL of 400 μM DPPH, 36 mL of 200 mM MES buffer, and 36 mL of 20% ethanol was prepared, and 2.7 mL was dispensed into each test tube (for 40 sample measurement). Further, (900-a) μL of 80% ethanol was added. An analytical sample aμL was added to this every 30 seconds and mixed by vortex (a = 0, 90, 180, 360, 540, 720 μL was added in this order). Absorbance at 520 nm was measured 20 minutes after addition of the analytical sample. Measurements are made sequentially according to the order of addition. The absorbance of the sample to which no analysis sample was added (a = 0) was defined as 100%, and the amount of analysis sample added corresponding to the absorbance of 50% was determined to obtain the DPPH 50% disappearance (μL / 3.6 mL assay). The results are shown in Table 4. Each analysis sample was prepared using 80% ethanol and adjusted to 10 mg / mL and 50 mg / mL.
表4から明らかなように、醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーの抗酸化活性は、生ニンニク(未処理ニンニク)に比べ顕著に高かった。抗酸化活性の向上は、ポリフェノールの増加に加えて、自己醗酵によってグルコースとアルギニンが反応し生産するメイラード反応物の影響によるものと示唆される。 As is clear from Table 4, the antioxidant activity of the fermented black garlic extract and the fermented black garlic powder was significantly higher than that of raw garlic (untreated garlic). It is suggested that the improvement in the antioxidant activity is due to the influence of the Maillard reaction product produced by the reaction of glucose and arginine by self-fermentation in addition to the increase in polyphenols.
〔実施例7〕(醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーのポリフェノール含量の評価)
醗酵黒ニンニク抽出液、醗酵黒ニンニクパウダー、青森県天間林産生ニンニク、中国産生ニンニクのそれぞれについて、下記のようにポリフェノール含量を評価した。
[Example 7] (Evaluation of polyphenol content of fermented black garlic extract and fermented black garlic powder)
For each of the fermented black garlic extract, fermented black garlic powder, Aomori Tenma Forest garlic, and Chinese garlic, the polyphenol content was evaluated as follows.
ポリフェノール含量をFolin-Denis法により測定した。すなわち、試料を精秤し、ウォーターバス中で1時間熱水抽出した。冷却後、100mLにメスアップし、ろ紙ろ過した。
得られたろ液を適宜希釈し、5mL分取した。Folin試薬5mL及び10%炭酸ナトリウム水溶液5mLを添加し、室温にて1時間発色させた後、700nmにおける吸光度を測定した。なお、2.5、5.0、10.0、20.0μg/mLに調整したタンニン酸溶液を標準として用いた。ポリフェノール(タンニン酸として)含量は次式により算出した。結果は表5に示した。
ポリフェノール(タンニン酸として)=A×V/5×B×10-6×100/S
A:タンニン酸濃度(μg/assay)
B:希釈率
S:試料採取量(g)
V:試料定容量(ml)
The polyphenol content was measured by the Folin-Denis method. That is, the sample was precisely weighed and extracted with hot water in a water bath for 1 hour. After cooling, the volume was increased to 100 mL and filtered through a filter paper.
The obtained filtrate was diluted appropriately and 5 mL was collected. After adding 5 mL of a Folin reagent and 5 mL of a 10% aqueous sodium carbonate solution and developing the color for 1 hour at room temperature, the absorbance at 700 nm was measured. A tannic acid solution adjusted to 2.5, 5.0, 10.0, 20.0 μg / mL was used as a standard. The content of polyphenol (as tannic acid) was calculated by the following formula. The results are shown in Table 5.
Polyphenol (as tannic acid) = A x V / 5 x B x 10 -6 x 100 / S
A: Tannic acid concentration (μg / assay)
B: Dilution rate
S: Sampling amount (g)
V: Sample constant volume (ml)
表5から明らかなように、醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーは生ニンニク(未処理ニンニク)に比べ、ポリフェノール含量が顕著に高かった。ポリフェノール含量の増加は、ニンニクが自己醗酵により加水分解され、配糖体から糖の部分がとれて、反応する活性基が増え、結果として測定する数値が増加したためであると示唆される。 As is clear from Table 5, the fermented black garlic extract and the fermented black garlic powder had a significantly higher polyphenol content than raw garlic (untreated garlic). It is suggested that the increase in the polyphenol content is due to the fact that garlic is hydrolyzed by self-fermentation, the sugar part is removed from the glycoside, the reactive group that reacts increases, and the measured numerical value increases as a result.
〔実施例8〕(醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーのイオウの含有量)
醗酵黒ニンニク抽出液、醗酵黒ニンニクパウダー、青森県天間林産生ニンニク、中国産生ニンニクのそれぞれについて、下記のようにイオウ含量を評価した。
[Example 8] (Sulfur content of fermented black garlic extract and fermented black garlic powder)
For each of the fermented black garlic extract, fermented black garlic powder, Aomori Tenma Forest garlic, and Chinese garlic, the sulfur content was evaluated as follows.
硫酸バリウム重量法(AOAC法)によりイオウ含量を測定した。すなわち、磁製るつぼに試料を秤量し、95%硝酸マグネシウム5mL及び硝酸3mLを加え、予備灰化後、500℃にて2時間灰化した。放冷後、水を少量加え、塩酸:水=1:1を10mL加え、ホットプレート上で内容物を溶解させた。これをろ紙ろ過し、200mLのコニカルビーカーに受け、水を加えて約100mLとした。指示薬メチルオレンジを数滴落とし、50%水酸化ナトリウムで中性とした後、塩酸:水=1:1を1mL加えて微酸性とした。煮沸させ、10%塩化バリウム溶液10〜15mLを加え、室温で一夜放置した。これをろ紙ろ過し、塩化物イオンの反応がなくなるように約200mLの水で洗った。沈殿をろ紙ごとるつぼに移し、500℃にて3時間灰化後、750℃にて20分間恒量化後のるつぼの重量を測定した。イオウ含量は次式により算出した。結果は表6に示した。
イオウ(%)=(W1−W2)/W×0.1374×100
W1:灰化後の総重量(g)
W2:るつぼの重量(g)
W :試料量(g)
The sulfur content was measured by the barium sulfate gravimetric method (AOAC method). Specifically, a sample was weighed in a porcelain crucible, added with 5 mL of 95% magnesium nitrate and 3 mL of nitric acid, pre-ashed, and then ashed at 500 ° C. for 2 hours. After cooling, a small amount of water was added, and 10 mL of hydrochloric acid: water = 1: 1 was added to dissolve the contents on a hot plate. This was filtered with filter paper, received in a 200 mL conical beaker, and water was added to make about 100 mL. After dropping a few drops of the indicator methyl orange and neutralizing with 50% sodium hydroxide, 1 mL of hydrochloric acid: water = 1: 1 was added to make it slightly acidic. It was boiled, 10-15 mL of 10% barium chloride solution was added, and it was left overnight at room temperature. This was filtered with a filter paper and washed with about 200 mL of water so that the reaction of chloride ions disappeared. The precipitate was transferred to the crucible together with the filter paper, and after ashing at 500 ° C. for 3 hours, the weight of the crucible after constant weighting at 750 ° C. for 20 minutes was measured. The sulfur content was calculated by the following formula. The results are shown in Table 6.
Sulfur (%) = (W1-W2) / W x 0.1374 x 100
W1: Total weight after ashing (g)
W2: crucible weight (g)
W: Sample amount (g)
表6から明らかなように、醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーは生ニンニク(未処理ニンニク)に比べ、イオウ含量が高かった。ニンニクの有効成分のほとんどはイオウ化合物である。本発明によれば、生ニンニクの有効成分であるイオウ化合物に着目しても、その含量をロスすることなく加工でき、ニンニク本来の有用性は発揮できるものと示唆される。 As is clear from Table 6, the fermented black garlic extract and the fermented black garlic powder had a higher sulfur content than raw garlic (untreated garlic). Most of the active ingredients in garlic are sulfur compounds. According to the present invention, even if attention is paid to a sulfur compound which is an active ingredient of raw garlic, it can be processed without losing its content, and it is suggested that the original utility of garlic can be exhibited.
〔実施例9〕(醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーのγ-グルタミル-S-アリルシステイン及びS-アリルシステイン含量)
醗酵黒ニンニク抽出液、醗酵黒ニンニクパウダー、青森県天間林産生ニンニク、中国産生ニンニクのそれぞれについて、高速液体クロマトグラフ―質量分析計(LC-MS/MS)により、γ-グルタミル-S-アリルシステイン含量及びS-アリルシステイン含量を評価した。
前処理は、試料約10gを精秤し、水:MeOH(=1:1)80mLに溶解・振とうし、遠心分離(8000rpm・5min)を行い、上澄みを250mLのメスフラスコに移した。この操作を全部で3回繰り返して250mLをメスアップ後、0.2μのフイルターでろ過した。結果は表7に示した。なお、LC-MS/MSの分析条件は、以下の通りである。
〈HPLC部〉 機種:alliance2695〔Waters〕
カラム:Mightysil RP-18 GP(φ2mm×50mm)
移動相:水-10mMギ酸アンモニウム-MeOH(85:10:5)
カラム温度:40℃
流速:0.2mL/min
注入量:5μL
〈MS部〉 機種:Quattro micro API〔MICROMASS〕
イオン法:ESI正イオンモード
[Example 9] (γ-glutamyl-S-allylcysteine and S-allylcysteine content of fermented black garlic extract and fermented black garlic powder)
Fermented black garlic extract, fermented black garlic powder, Amamori Tenmabayashi garlic, and Chinese garlic were analyzed by high-performance liquid chromatograph-mass spectrometer (LC-MS / MS) for γ-glutamyl-S-allylcysteine content And S-allyl cysteine content was evaluated.
In the pretreatment, about 10 g of a sample was precisely weighed, dissolved and shaken in 80 mL of water: MeOH (= 1: 1), centrifuged (8000 rpm, 5 min), and the supernatant was transferred to a 250 mL volumetric flask. This operation was repeated a total of 3 times to make up 250 mL, and then filtered through a 0.2 μ filter. The results are shown in Table 7. The analysis conditions for LC-MS / MS are as follows.
<HPLC part> Model: alliance2695 [Waters]
Column: Mightysil RP-18 GP (φ2mm × 50mm)
Mobile phase: water-10 mM ammonium formate-MeOH (85: 10: 5)
Column temperature: 40 ° C
Flow rate: 0.2mL / min
Injection volume: 5μL
<MS part> Model: Quattro micro API [MICROMASS]
Ion method: ESI positive ion mode
表7より明らかなように、醗酵黒ニンニク抽出液及び醗酵黒ニンニクパウダーは生ニンニク(未処理ニンニク)に比べ、γ-グルタミル-S-アリルシステイン含量が低く、ガン予防、コレステロール抑制、動脈硬化改善、心疾患予防、アルツハイマー防止などに効果があるとされているS-アリルシステイン含量が高かった。これは、自己醗酵において、酵素γ-グルタミルトランスフェラーゼの作用により、γ-グルタミル-S-アリルシステインがS-アリルシステインに変化するためであると推測される。 As is clear from Table 7, fermented black garlic extract and fermented black garlic powder have lower γ-glutamyl-S-allylcysteine content than raw garlic (untreated garlic), preventing cancer, improving cholesterol, and improving arteriosclerosis S-allylcysteine content, which is said to be effective in preventing heart disease and preventing Alzheimer's, was high. This is presumably because γ-glutamyl-S-allylcysteine is changed to S-allylcysteine by the action of the enzyme γ-glutamyltransferase in self-fermentation.
なお、上記の醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーの原料となる醗酵黒ニンニクとの比較のため、上記と同じ中国産生ニンニクを温度50℃、湿度60%に設定して上記と同じ熟成室に30日間放置した。その結果、生ニンニクは腐敗し、形が崩れ泥状になっていた。また、温度90℃、湿度50%に設定して熟成室に30日間放置した。その結果、生ニンニクは乾燥し、淡褐色の硬い石状になっていた。 In addition, for comparison with fermented black garlic, which is the raw material of the above fermented black garlic extract or fermented black garlic powder, the same aging room as above with the same Chinese garlic produced as above set at a temperature of 50 ° C and a humidity of 60% Left for 30 days. As a result, the raw garlic rotted, and its shape collapsed and became muddy. The temperature was set to 90 ° C. and the humidity was set to 50%, and the mixture was left in an aging room for 30 days. As a result, the raw garlic was dried and turned into a light brown hard stone.
〔実施例10〕以下、醗酵黒ニンニク抽出液又は醗酵黒ニンニクパウダーを配合した飲食物及び医薬の具体例を示した。 [Example 10] Hereinafter, specific examples of foods and drinks and pharmaceuticals containing fermented black garlic extract or fermented black garlic powder are shown.
(配合例1)清涼飲料水
下記の原料を清涼飲料水の常法により処理し、醗酵黒ニンニク抽出液を含有する清涼飲料水を製造した。
醗酵黒ニンニク抽出液 50 mg
果糖ブドウ糖液糖 4000 mg
ハチミツ 2000 mg
保存料(安息香酸ナトリウム) 18 mg
ローヤルゼリー抽出物 14 mg
調味料(タウリン) 5 mg
リジン 50 mg
トレオニン 10 mg
ビタミンC 50 mg
ビタミンB1 1.1 mg
ビタミンB2 1.2 mg
ビタミンB6 1.6 mg
ニコチン酸アミド 17 mg
マカ抽出液 30 mg
ウコン抽出液 10 mg
着色料(カラメル) 6 mg
酸味料 適量
香料 適量
(Composition example 1) Soft drink The following raw material was processed by the conventional method of soft drink, and the soft drink containing fermented black garlic extract was manufactured.
Fermented black garlic extract 50 mg
Fructose glucose liquid sugar 4000 mg
Honey 2000 mg
Preservative (sodium benzoate) 18 mg
Royal jelly extract 14 mg
Seasoning (taurine) 5 mg
Lysine 50 mg
Threonine 10 mg
Vitamin C 50 mg
Vitamin B 1 1.1 mg
Vitamin B 2 1.2 mg
Vitamin B 6 1.6 mg
Nicotinamide 17 mg
Maca extract 30 mg
Turmeric extract 10 mg
Coloring agent (caramel) 6 mg
Acidulant appropriate amount Fragrance appropriate amount
(配合例2)飴
下記の原料を飴の常法により処理し、醗酵黒ニンニク抽出液を含有する飴を製造した。
醗酵黒ニンニク抽出液 15 g
砂糖 200 g
水飴 30 g
水 60 g
(Formulation example 2) Koji The following raw materials were processed by the usual method of koji, and koji containing fermented black garlic extract was produced.
Fermented black garlic extract 15 g
200 g sugar
Minamata 30 g
60 g water
(配合例3)ドレッシング
下記の原料をドレッシングの常法により処理し、醗酵黒ニンニク抽出液を含有するドレッシングを製造した。
醗酵黒ニンニク抽出液 5 g
しょうゆ 30 g
酢 30 g
胡麻油 30 g
葱のみじん切り 5 g
生姜のみじん切り 5 g
(Composition example 3) Dressing The following raw material was processed by the conventional method of dressing, and the dressing containing a fermented black garlic extract was manufactured.
Fermented black garlic extract 5 g
Soy sauce 30 g
Vinegar 30 g
Sesame oil 30 g
Chopped firewood 5 g
5 g chopped ginger
(配合例4)糖衣錠
醗酵黒ニンニクパウダー 20 mg
粉末黒酢 10 mg
黒酢もろみ末 10 mg
トレハロース 3 mg
グリセリン 8 mg
クエン酸 5 mg
クエン酸カルシウム 3 mg
セラック 微量
(Formulation 4) Sugar-coated tablets Fermented black garlic powder 20 mg
Powdered black vinegar 10 mg
Black vinegar moromi powder 10 mg
Trehalose 3 mg
Glycerin 8 mg
Citric acid 5 mg
Calcium citrate 3 mg
Small amount of shellac
(配合例5)パン
下記の原料をパンの常法により処理し、醗酵黒ニンニクパウダーを含有するパンを製造した。
醗酵黒ニンニクパウダー 25 g
強力小麦粉 345 g
ドライイースト 3.4 g
砂糖 23 g
食塩 7.5 g
スキムミルク 7.5 g
鶏卵 38 g
無塩バター 19 g
水 240 g
(Formulation example 5) Bread The following raw material was processed by the usual method of bread, and the bread containing fermented black garlic powder was manufactured.
Fermented black garlic powder 25 g
Powerful flour 345 g
Dry yeast 3.4 g
23 g sugar
Salt 7.5 g
Skim milk 7.5 g
Egg 38 g
Unsalted butter 19 g
240 g water
(配合例6)うどん
下記の原料をうどんの常法により処理し、醗酵黒ニンニクパウダーを含有するうどんを製造した。
醗酵黒ニンニクパウダー 20 g
小麦粉 280 g
食塩 15 g
水 145 g
(Formulation example 6) Udon The following raw materials were processed by the usual method of udon, and the udon containing fermented black garlic powder was manufactured.
Fermented black garlic powder 20 g
Flour 280 g
Salt 15 g
145 g water
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