JP2007119377A - Composition having pharmaceutical effect or seasoning property and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition which is obtained from an Allium plant, especially long green onion, has a pharmaceutical effect property and/or a seasoning property, and can highly probably be used as a raw material for seasonings and health foods and as a medicine for preventing or treating various diseases. <P>SOLUTION: This composition having the pharmaceutical effect property and/or the seasoning property and having functions such as a hypocholesterolemic function, a fatty liver-preventing and treating function and an erythrocyte deformation-improving function is obtained by applying a high concentration acetic acid immersion treatment to the Allium plant, especially the long green onion or Welsh onion of disposal target, and fractionating a dilute acetic acid-soluble ingredient. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a composition having medicinal properties and a taste obtained by extraction from a leek plant, and the composition is used as a raw material for health foods and seasonings, and further, a pharmaceutical for preventing or treating various diseases. It relates to the preparation of health foods, seasonings, and pharmaceutical preparations.

  Leek plants, especially leeks, are extremely cold-resistant, resistant to heat and dryness, have a unique smell and pungent taste, and have vitamins A, B, C, etc., so they are essential for Japanese cuisine. It is a vegetable that has been cultivated for years from Hokkaido to Kyushu, and has developed unique varieties.

  In China, these leeks have long been used as an ingredient in traditional Chinese medicines and have been shown to be effective for buds, berries, leaves, roots, and cores. For example, decoction is used for high fever, bone pain, and facial edema. Treatments such as washing with decoction for metal wounds and insect snake bites, and applying boiled and ground leaves and stems for foot edema have been used.

  Accordingly, attempts have been made for a long time to separate and acquire these active ingredients contained in the leek. Recently, for example, JP 2002-186449 (Patent Document 1) discloses that an extract extracted with an alcohol solvent exhibits a strong taste while maintaining a fresh and natural flavor, and has a bitter taste. It does not have a roasted feeling, and has a bright color, so it must be a highly useful flavoring material that can be used not only for dark-colored foods such as curry and beef stew but also for white sauce. It is shown.

  JP-A-2005-261396 (Patent Document 2) discloses a monosulfide compound that inhibits the Maillard reaction, which causes browning of foods, particularly proteins, such as milk powder, dried eggs, and meat. It can be extracted from the essential oil fraction of leek plants obtained by extraction with a solvent, has high safety and is suitable as a treatment agent for preventing browning of products applied to humans such as foods and pharmaceuticals. It is shown.

  On the other hand, the present applicant previously described in Japanese Patent No. 3108059 (Patent Document 3), as a method for producing a physiologically active peptide-containing material using a plant as a raw material, an incubation treatment with an acetic acid aqueous solution, and this acetic acid treated product. We proposed a method for obtaining peptide-containing products by protease treatment to reduce the molecular weight of proteins.

  In Patent Document 3, a physiologically active peptide-containing material is obtained from refined pearl barley powder by a method for producing a physiologically active peptide-containing material. This composition has an angiotensin I converting enzyme (ACE) inhibitory action, tumor necrosis factor ( In addition to having excellent physiological functions such as TNF) -α production regulating action, active oxygen production inhibitory action, and antitumor action, it is disclosed that the composition is derived from a natural product and is very safe.

  Further, the present applicant has disclosed a method for obtaining a peptide-containing product by reducing the molecular weight of a protein, which can be applied to various kinds of plants.

That is, plant whole seeds such as plant seeds, bark, real part, stem part, root part, leaf part, each part thereof, or one part thereof, or a processed product thereof, or a food obtained therefrom or a processed product thereof are used.
Plant seeds include pearl barley, rice, barley, wheat, oats, bare barley, buckwheat, soybeans, peas, broad beans, peanuts, green beans, corn, cucumbers, sesame seeds, millet, millet, sunflower, hemp seeds, vegetable seeds Others are mentioned.
The treated products include those obtained by threshing plant seeds, those obtained by pulverizing them, defatted products such as moss, yokoinin, hexane, water extraction residue, organic solvent extracts such as alcohol, and other various treatments of plant seeds. Things.
In addition, the processed products include sake lees, okara, shochu, soy sauce manufacturing koji, starch manufacturing koji, and other by-products when processing and processing plant seeds or processing products thereof to produce foods and drinks. .
JP 2002-186449 A (claims, effects of the invention) Japanese Patent Laying-Open No. 2005-261396 (claims, effects of the invention) Japanese Patent No. 3108059 (Claims)

  The inventors examined the expansion of the method of obtaining the peptide-containing product by reducing the molecular weight of the previously proposed protein, and the leeks that are also used as the above-mentioned herbal medicines, in particular, discarded or used as non-standards. We will investigate whether an active ingredient is extracted from the leaf sheath of the leek that is discarded without delay, and propose a method that can use leek more effectively than before.

In order to achieve the above-mentioned problems, the inventors have conducted intensive studies, treating leeks with high-concentration acetic acid, solubilizing water-insoluble proteins and tissue components, and being soluble in dilute acetic acid. The obtained composition is excellent in various physiological activities, for example, production of TNF-α, improvement of liver function, increase of good cholesterol, prevention of destruction of pancreatic islets due to oxidative stress, and excellent taste. Furthermore, the present inventors have found that a composition obtained by treating the composition with a protease and reducing the molecular weight of the protein contained therein is further excellent in taste, and thus completed the present invention.

That is, the invention according to claim 1 of the present invention is
It is an extract from a leek plant, which is soluble in dilute acetic acid, and has a medicinal effect and / or taste.

The invention according to claim 2 of the present invention is
It is an extract from a leek plant that has been subjected to a soaking treatment with high-concentration acetic acid, and is a composition having medicinal properties and / or a taste that is soluble in dilute acetic acid.

The invention according to claim 3 of the present invention is
In the composition having medicinal properties and / or tastes according to claim 1 or 2,
The leek plant is
It is characterized by being a leek.

The invention according to claim 4 of the present invention is
In the composition having medicinal properties and / or tastes according to claim 1 or 2,
The dilute acetic acid is
The concentration is 4 to 5% by mass.

The invention according to claim 5 of the present invention is
In the composition having medicinal properties and / or tastes according to claim 2,
The high concentration acetic acid is
The concentration is 40% by mass or more.

The invention according to claim 6 of the present invention provides
In the composition having medicinal properties and / or tastes according to claim 1 or 2,
The extract is
The molecular weight of the protein contained in the extract is reduced by hydrolysis using a proteolytic enzyme.

The invention according to claim 7 of the present invention provides
The chopped green onions are immersed in a high-concentration acetic acid aqueous solution, diluted with water to dilute, and the active ingredients present in the dilute acetic acid aqueous solution obtained by removing the insoluble matter are obtained separately. It is a method for producing a composition having medicinal properties and / or taste.

The invention according to claim 8 of the present invention provides
In the manufacturing method of the composition which has a medicinal property and / or a taste property of Claim 7,
The active ingredient is
It is characterized by hydrolyzing by adding a proteolytic enzyme to lower the molecular weight of the protein in the active ingredient.

The invention according to claim 9 of the present invention provides
In the manufacturing method of the composition which has a medicinal property and / or a taste property of Claim 8,
The proteolytic enzyme is
It is characterized by being denapsin.

Since the composition having medicinal properties and / or palatability according to the present invention is extracted with acetic acid, more saccharides are extracted and the taste is superior than those using conventional solvents such as normal hexane. In addition, there is no concern about safety due to the residual organic solvent, and it has the following characteristics.
1) The leek enzyme (alliinase / oxidase) is inactivated, and the umami component (alliin) is stabilized and changes in taste are prevented.
2) Many water-insoluble components are solubilized and extracted.
3) No heat denaturation due to high temperature treatment.
4) A water-soluble substance and a fat-soluble substance coexist.
5) Protease digestibility increases due to protein denaturation and solubilization.
6) Has sterilization / oxidation inhibitory effect.
7) A large amount of long-term storage is possible.
8) The preparation cost is low.
Therefore, the composition of this invention has a possibility of being widely used as a seasoning for business use and further as a component of various household seasonings.

Furthermore, in addition to the above-mentioned properties, the composition having medicinal properties and / or tastes according to the present invention has the following excellent functions for living organisms. It can be used effectively as a raw material.
1) Good cholesterol increase function 2) Improvement of liver function 3) Improvement function of erythrocyte deformability (improvement to so-called blood freezing condition)
4) Hypoglycemic action 5) Prevention of islet destruction by oxidative stress

  In addition, since the composition having medicinal properties and / or tastes according to the present invention can be used in a powder state or as a dilute acetic acid aqueous solution, in order to develop the above effects, as a health food or a medicine It can be used efficiently and effectively when used.

Furthermore, the medicinal properties and / or taste-enhancing compositions of the present invention can effectively use non-standard onions that have significantly reduced market value, as well as onions to be disposed of, as raw materials for health foods and pharmaceuticals. This is an excellent effect that makes it possible to make the most effective use of leeks.

  In this invention, the composition having medicinal properties and / or a taste is extracted from a leek plant, and as a leek plant, a leek, a leek, an onion, a garlic, a leek, a leek, a bamboo grass as cultivated species , Charlotte, nobil as wild species, chives, garlic garlic, etc. are used, and leeks are particularly preferred for the present invention, although their varieties have some differences in performance.

As the leeks, both green leeks and white leeks varieties and strains show the effect of medical function. Among them, green leeks have particularly high medical functionality. Moreover, as a leek, what has been abandoned as non-standard in the market can be used without discrimination.
In addition, a leek consists of leaves, flower stems, leaf sheaths, and flower balls, and some differences are recognized in terms of medical functions for each part, but this is not a problem that causes problems in use.

  At the time of extraction, these leek plants are used, and the parts such as leaves, flower stems, leaf sheaths, flower spheres are not divided into parts, and there is no problem even if they are used as a whole.

  The composition having the medicinal properties and / or the taste of the present invention is extracted from a leek plant. In the extraction, first, the leek plant is immersed in high-concentration acetic acid to obtain a high concentration. It is necessary to contact with concentrated acetic acid. The leek plant is washed with tap water, deionized water, etc., cut into pieces with a knife, and soaked.

  As the high concentration acetic acid, 90% acetic acid such as concentrated acetic acid obtained by concentrating synthetic acetic acid or brewed vinegar, particularly 90% acetic acid for food addition is preferable. A higher concentration of acetic acid is preferred, but from the viewpoint of ease of treatment, water is added after addition of high concentration acetic acid, and diluted twice, that is, with acetic acid having a substantial concentration of 45%, and further contained in the leeks. Even if the concentration of acetic acid is reduced to about 40% due to the moisture to be generated, no serious problem occurs in the present invention.

  The amount of acetic acid used in the acetic acid immersion treatment is desirably an amount that can uniformly immerse the surface of the leek plant in consideration of the effect of the immersion treatment and the treatment (removal) after the extraction. Depending on the thickness and the degree of shredding, high-concentration acetic acid having a mass of 10 to 20% of the mass of the leek plant is used.

  The acetic acid immersion treatment does not need to be performed by heating, and may be performed at room temperature. Furthermore, in order to avoid alteration, decay, etc. of leeks, it may be carried out at a cold temperature, for example, in a refrigerator, and is usually carried out at a temperature of 5 to 50 ° C. Although it depends on the immersion temperature, the immersion time may be about 10 hours to 1 month, and it may be left still during that time, or it may be gently stirred occasionally or at regular intervals. Is desirable.

  For systems where acetic acid and leek plants that have undergone acetic acid immersion treatment coexist, add water as necessary, homogenize using a homogenizer, and add water while stirring the resulting uniform. After the final acetic acid concentration of the system is 4 to 5% by mass, it is separated into an insoluble substance and a solution.

  The composition having the medicinal properties and / or the taste of the present invention can be obtained by concentrating the solution from which the insoluble matter is separated and further removing the solvent by means such as lyophilization. The obtained composition can be divided into those that are soluble in 80% ethanol and those that are insoluble, and there are some differences in their properties. In the present invention, the two are combined into the composition of the present invention. .

  What is extracted in this way is a composition comprising various components, but is soluble in dilute acetic acid, particularly 4.5% strength dilute acetic acid. Moreover, it has various characteristics described later, and is effectively used as a material for seasonings and health foods, and as a raw material for pharmaceuticals.

  In addition, since the composition of the present invention contains proteins, by reducing the molecular weight thereof, it becomes more excellent in taste and medicinal properties. Therefore, it is preferable to add a proteolytic enzyme to hydrolyze the protein present in the composition to lower the molecular weight.

  As the proteolytic enzyme to be used, various types of proteases such as pepsin, papain, bioplase (promeline), thermolysin, and denapsin, including commercially available products, such as commercially available products can be used. They can be used alone or in combination, and when used in combination, they may be used simultaneously or sequentially. For the present invention, preferred is denapsin, an acidic protease.

  The decomposition temperature at the time of protein decomposition is determined by the activation temperature of the proteolytic enzyme used. For example, in the case of a protease, the temperature is about 45 to 60 ° C., and the degradation time is determined according to the proteolytic ability and degradation temperature of the proteolytic enzyme used, but is usually about 15 to 30 hours.

  As described above, the composition of the present invention has physiological functions such as a cholesterol-lowering function, a function of preventing and healing fatty liver, and a function of improving erythrocyte deformability (improvement to a so-called blood freezing state). Moreover, it is derived from natural food-related plants and has no safety problems. Therefore, it can be used by adding to foods and drinks such as foods and drinks for specific health, health foods, health drinks, and nutritional foods as well as normal foods and drinks. Further, it can be used as a seasoning and as various pharmaceuticals.

  In this case, the composition can be used in any form of solid (powder, granule, etc.), concentrate, paste, liquid or suspension. Moreover, it can be used as a tablet, a capsule, a granule, a powder, a syrup, etc. by utilizing the excipient | filler, binder, etc. which are used in a seasoning, health food, or a pharmaceutical formulation.

This composition is of natural origin, and since it has been used for many years as a traditional Chinese medicine or food, it has no or very low toxicity and exhibits excellent safety. There is no special limitation, even if it is an elderly person, an infant, or a sick person, it can be taken for a long time.

<Preparation of composition 1>
The single white leek CA (branch type) and the single leek FS provided by the Chikuzen Asakura Agricultural Cooperative Association were washed with water, and then separated into leaf, flower stem, leaf sheath, and flower bulb parts, respectively, to obtain extraction raw materials.
High-concentration acetic acid immersion treatment and extraction using leaves of single white leek CA were performed as follows.
First, after shredding 1206 g of a white leek CA leaf (water content 91.5%, dry weight 103 g) to a length of about 5 cm, add 200 ml of 90% acetic acid for food addition and 200 ml of water, with occasional stirring. Left at room temperature overnight.
Homogenization was performed with a homogenizer while adding water to high-concentration acetic acid containing leaves of a single white leek CA after soaking overnight. Finally, a total amount of 4 L of homogenate was obtained. The filtrate obtained by centrifugal filtration (3000 rpm × 5 minutes) with a centrifuge equipped with an 80-mesh nylon filtration membrane was combined with the washing liquid after washing the residue, and again centrifuged with a centrifuge (8000 rpm). X10 minutes) and the supernatant was collected.
The pH of the supernatant was 3.08 and the acetic acid concentration was 4.5%.
After adding an 80% ethanol solution to this dilute acetic acid aqueous solution and stirring, the precipitated precipitate and the solution are separated, and the solution is concentrated to 110 ml under reduced pressure, and 4 times the volume of ethanol is added again to precipitate the precipitate. 42.4 g of powder was obtained from Kiyoshi by vacuum concentration and freeze-drying.
The amount precipitated with 80% ethanol was 11.6 g. In this invention, both were combined to make an invention composition.
Similarly, the results of the same extraction for each of the flower stems and leaf sheaths of the single white leek CA and the single leek FS and the mixture of both flower balls are shown in Table 1 together with the above results. .
The numerical value in parentheses in the “total yield column” in Table 1 indicates the amount dissolved in an 80% ethanol solution.

  100 mg of a diluted acetic acid / 80% ethanol soluble composition powder obtained from the leaves of a single white leek CA as described above was dissolved in a 1% acetic acid solution, and the supernatant obtained by centrifugation was preliminarily treated with 1% acetic acid. Apply to a Bio-Gel P-10 (2 × 30 cm) column washed with the solution, elute with 1% acetic acid solution, take 40 drops (about 1.3 ml) in a test tube, and measure the absorbance at 230 nm and 280 nm The elution pattern in the gel filtration method is shown in FIG.

<Preparation of composition 2>
Acetic acid soaking conditions for a mixture of three white onions (one white onion CA, one onion FS and one other) and two green onions (soft and solid) provided by the Chikuzen Asakura Agricultural Cooperative The composition of the present invention was obtained in the same manner as in Preparation 1 except for 15 to 25 days. The results are shown in Table 2. Moreover, the elution pattern in a gel filtration method is shown in FIG. 2, FIG.

<Preparation of composition 3>
The composition of the present invention was obtained in the same manner as in Preparation 1 except that the following five types of leek provided from the Fukuoka Prefectural Agricultural Experiment Station were subjected to acetic acid immersion conditions for 20 hours. The results are shown in Table 3.
Types of leeks 1: FDH (variety name, the mainstream variety of Hakata universal leeks)
2: 04-133 (Strain name, Hakata universal spring onion type with strong hotness)
3: 04-170 (train name, Hakata universal spring onion type with little pungency)
4: Kinchou (variety name, Nebuka Onion)
5: Kurume-city conventional system (system name, medium leek)

Composition Preparation 4
198 g (yield 3) of the composition of the present invention was obtained in the same manner as in Preparation 1 except that 5280 g of green onion waste provided by Asakura Agricultural Cooperative and 792 ml of 90% acetic acid were used and the acetic acid soaking conditions were changed to refrigerator for 4 days. .75%). The analysis results of the composition are shown in Table 4.

As apparent from the above, since the composition of the present invention contains a protein, the protein was decomposed by a proteolytic enzyme as follows in order to reduce the molecular weight of the protein.
That is, 150 ml of deionized water was added to 30 g of the composition obtained in Preparation 4 above, adjusted to pH 4.0 with dilute hydrochloric acid solution, 0.6 g of Denapsin 10P, an acidic protease, was added, and incubated at a temperature of 50 ° C. for 3 hours. .
The decomposition solution was concentrated under reduced pressure and then lyophilized to obtain a denapsin decomposition product. The elution pattern in the gel filtration method is shown in FIG. As is apparent from this figure, the fractions 3, 4 and 5 were increased, the protein was reduced in molecular weight, and the taste of the composition was improved.

<Antioxidant properties of the composition>
The antioxidant action of the dilute acetic acid / 80% ethanol-soluble composition obtained from the leaves, flower stems, leaf sheaths and flower balls of the single white leek CA obtained as described above was determined by the β-carotene method, that is, each sample solution 0 .Beta.-in the case where 4.9 ml of linoleic acid-.beta.-carotene solution was added to a spectrophotometer test tube cell dispensed with 1 ml, stirred, and incubated in a constant temperature bath at a temperature of 50.degree. The degree of fading of carotene is determined from the absorbance at 470 nm, and converted to butylhydroxyanisole (BHA) concentration to determine the degree of oxidation. The results are shown in FIG.

<Immune function of the composition>
In order to determine the activation of immune function by the 80% ethanol-soluble composition extracted from the leaf of single white leek CA obtained as described above and the leaf sheath, TNF (Tumor Necrosis Factor) -α of the composition The production amount of was measured.
As is clear from the results shown in FIG. 6, it is recognized that the immune function can be activated by the composition. The production amount of TNF-α was measured as follows.

<Method for measuring production amount of TNF-α>
To 500 μl of a neutrophil suspension (1.0 × 10 ⁶ cells / ml) separated from human peripheral blood, 10 μl of a composition solution with different dilutions is added, and the temperature is 37 ° C. in an incubator containing 5% CO _2. After incubating for 6 hours, the mixture was centrifuged at 1500 rpm for 5 minutes, and the amount of TNF-α in 400 μl of the obtained supernatant was quantified by ELISA.

<Functional measurement in living body>
The effect of the medicinal composition of this invention on the living body was examined using rats.
As rats, 9-10 week-old male Wistar SPF rats administered with STZ (streptozotocin) that induces diabetes were examined.
As the composition of the present invention, the following four types of leeks were used as extraction sources, and feed MF manufactured by Oriental Yeast Co., Ltd., to which 5% of the composition extracted therefrom was added, was given to rats for 3 weeks.

<Extraction source>
1. White leek: FS, CA (Fukuoka Horticultural Development Line)
2. Green onion: FDH (Hakata Universal Allium Mainstream Variety)
3. Green onion: 3039 (Fukuoka Horticultural Development Line)
4). Green onion: 206 (Kurume City conventional system)

The items to be examined are as follows.
1. Body weight Blood glucose level (tail vein: using Glutest Ace R (Sanwa Chemical Laboratory))
3. Blood test (blood collection from abdominal aorta: general blood, biochemical test (plasma), red blood cell deformability)
4). Microscopic observation

  In addition, erythrocyte deformability is a filter characteristic using a nickel mesh filter developed by the inventor as an alternative to the conventional method using a nucleopore filter that has difficulty in quantification and reproducibility. Was measured by the following method.

  The nickel mesh is a nickel thin film filter made by combining a photoresist method and a special plating method. The number, shape and distribution of micropores are not only constant, but also 100 times by ultrasonic cleaning for several seconds. The above reuse is possible. In addition, the edges of the nickel mesh micropores are smooth and tapered so that the leukocytes are not mechanically affected and the micropores have no fusion or branching. Due to these characteristics, the following method maintains high quantitativeness and reproducibility.

<Erythrocyte deformability measuring method (Nickel mesh filtration method)>
In the test, a nickel mesh holder was connected to a vertically standing glass tube via a Tygon tube, and was prepared with physiological saline adjusted with a HEPES buffer, usually at a height of 15 cm (height: h). Filter the erythrocyte suspension.

Around the glass tube, constant temperature water is refluxed to keep the sample at a constant temperature.
The pressure drop during the filtration of the sample is continuously detected by a pressure (P) transducer installed at the zero level of the glass tube, and this is taken into a personal computer through an amplifier and an AD converter, and the flow rate (Q) ).
The flow rate is converted from pressure to height (P = ρgh), the differential value (dh / dt) of the height-time (ht) curve is taken, and this is multiplied by the cross-sectional area (a) of the glass tube. (Q = dh / dt · a).
Using the pressure-flow curve of a control solution that does not contain blood cells (HEPES buffer-adjusted saline: Newtonian fluid) as a control, examine the pressure-flow curve of the erythrocyte suspension, and at a certain pressure (usually 100 mm · H ₂ O) Erythrocyte deformability is evaluated based on the flow rate (%) of the erythrocyte suspension relative to the flow rate of the control solution.

  FIG. 7 to FIG. 14 show the results of examining the influence of the composition of the present invention in rats administered with STZ and causing abnormalities such as diabetes. Significance (0.01%, 0.05%) By the administration of the composition of the present invention, HGB (FIG. 7), HDL cholesterol (FIG. 8), LDL cholesterol (FIG. 9), AST (FIG. 10), ALT (FIG. 11), urea It is recognized that it contributes to the improvement of the characteristics of nitrogen (FIG. 12), uric acid (FIG. 13), and red blood cell deformability (FIG. 14).

Microscopic tissue observation was performed on the model animal pancreatic islets of Langerhaus (La Isle), and the effect of the composition of the present invention on the pancreas of diabetes was investigated.
Under microscopic observation, each animal was sacrificed under ether anesthesia, and then the pancreas was removed and fixed by immersion in Buan disease for 3 days. The pancreatic tissue specimen was thoroughly washed with 0.1 M phosphate buffer containing 7% sucrose, immersed in a freezing packing agent, and frozen at -20 ° C.
A cryostat specimen having a thickness of 14 μm was prepared, placed on a slide glass coated with chromium alum / gelatin, and dried in cold air for a short time.
The specimen was prepared from mouse anti-insulin monoclonal antibody (Lab Vision., Co., CA, USA) as a primary antibody and horse anti-mouse IgG-FITC (Vector Labs, CA, USA) as a secondary antibody. B cells were immunostained and performed with an epifluorescence microscope (Carl Zeiss, Germany).

FIG. 15 shows a pancreatic islet of a normal control rat, FIG. 16 shows an immunostaining image of an insulin antibody of a pancreatic islet of a diabetes model rat, and FIG. 17 shows a mild diabetes model rat after administration of the composition of the present invention. Immunostained images of insulin pancreatic islets are shown.
As each model rat, comparatively mildly diabetic rats having a blood glucose level of less than 200 mg / dl were selected and compared.

As is apparent from these figures, B cells in the pancreatic islets of normal control rats are uniformly dispersed in the pancreatic islets, exhibiting a relatively strong immunocoloration, and the pancreas of diabetic model rats La Islet B cells are localized in the periphery of the pancreatic La Islet, and many of these cells have weak immune responses due to significant degeneration, and insulin-positive B cells remain in the center. ing.
In contrast, in the mildly diabetic model rat after administration of the composition of the present invention, B cells that are slightly deformed due to weak or weak immune responses of B cells are observed in the peripheral area of the pancreatic islet. Is inhibited from being destroyed or denatured by administration of the composition.

As is apparent from these results, the composition of the present invention has a cholesterol lowering function, a fatty liver prevention and healing function, a red blood cell deformation improving function, a so-called blood smoothing function.

Since the composition of the present invention has the above-mentioned excellent characteristics and can be supplied as a powder or aqueous solution, it is highly likely to be widely used in the seasoning-related industry, health food industry and pharmaceutical industry. It is.

It is an elution pattern figure in the gel filtration method of the composition (80% ethanol soluble content) extracted from the leaf of the single white leek CA of this invention. It is an elution pattern figure in the gel filtration method of the composition extracted from the white leek of this invention. It is an elution pattern figure in the gel filtration method of the composition extracted from the green onion (solid) of this invention as a raw material. It is the elution pattern figure in the gel filtration method of the composition which extracted the green onion of this invention as a raw material, and also made the content protein low molecular weight. It is a figure which shows the antioxidant activity of the composition (80% ethanol soluble part) obtained from the leaf, flower stem, leaf sheath, and flower ball of single white leek CA. It is the figure which showed the change of the production amount of TNF- (alpha) by the addition of the composition (80% ethanol soluble part) extracted from the leaf and leaf sheath of single white leek CA of this invention. It is a figure which shows the change of HGB in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of the HDL cholesterol in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of LDL cholesterol in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of AST in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of ALT in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of the urea nitrogen in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of the uric acid in the blood of a model rat by administration of the composition of this invention. It is a figure which shows the change of the erythrocyte deformability of a model rat by administration of the composition of this invention. It is a microscope picture which shows the immuno-staining image by the insulin antibody of the pancreas la islet of a normal control rat. It is a microscope picture which shows the immuno-staining image by the insulin antibody of mild diabetes model rat pancreas la islet. It is a microscope picture which shows the immuno-staining image by the insulin antibody of the mild diabetes model rat pancreas la islet after administration of the composition of this invention.

Explanation of symbols

    None

Claims (9)

An extract from a leek plant, which is soluble in dilute acetic acid and has a medicinal effect and / or a taste-enhancing composition. An extract from a leek plant that has been soaked with high-concentration acetic acid, and is soluble in dilute acetic acid, and has a medicinal effect and / or a taste-enhancing composition. The leek plant is
It is a leek, The composition which has a medicinal property and / or a taste property of Claim 1 or 2 characterized by the above-mentioned. The dilute acetic acid is
3. The composition having medicinal properties and / or a taste according to claim 1, wherein the concentration thereof is 4 to 5% by mass. The high concentration acetic acid is
3. The composition having medicinal properties and / or a taste according to claim 2, wherein the concentration is 40% by mass or more. The extract is
3. The composition having medicinal properties and / or a taste according to claim 1, wherein the molecular weight of the protein contained in the extract is reduced by hydrolysis using a proteolytic enzyme. object. The chopped green onions are immersed in a high-concentration acetic acid aqueous solution, diluted with water to dilute, and the active ingredients present in the dilute acetic acid aqueous solution obtained by removing the insoluble matter are obtained separately. A method for producing a composition having medicinal properties and / or taste. The active ingredient is
8. The method for producing a composition having medicinal properties and / or a taste according to claim 7, wherein a protein in the active ingredient is hydrolyzed by adding a proteolytic enzyme to lower the molecular weight. The proteolytic enzyme is
It is a denapsin, The manufacturing method of the composition which has a medicinal property and / or a taste property of Claim 8 characterized by the above-mentioned.