JP2007077034A - Cosmetic product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic material having excellent skin whitening effect and antioxidation activity and provide a cosmetic product having excellent skin whitening effect. <P>SOLUTION: The cosmetic product contains a skin whitening component comprising (A) 4-ethyl-2-methoxyphenol, (B) 2,6-dimethoxyphenol and (C) a phenyl propanoid glycoside compound in bamboo vinegar or their mixture. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a cosmetic material excellent in whitening effect and antioxidant ability.

  In recent years, the amount of ultraviolet rays falling on the earth has increased rapidly, and there are concerns about adverse effects on the human body. While the ultraviolet rays have useful effects such as vitamin D biosynthesis and bactericidal effects, active oxygen and free radicals are generated in the body when irradiated with a large amount of ultraviolet rays, aging, arteriosclerosis, cancer, skin degeneration Symptoms such as appear. It has been clarified that about 90% of these modern diseases involve active oxygen and free radicals. On the other hand, from a cosmetic point of view, it is also known that there are many harmful effects such as promoting the formation of spots and buckwheat and promoting aging of the skin.

Many factors have been cited for the production of melanin, the cause of stains and freckles. One of them is known to involve enzymes, and among them, it relates to a component in bamboo vinegar that inhibits the activity of tyrosinase, the key enzyme for melanin production. In addition, cosmetics are used every day, and are applied directly to the skin for a long time. Therefore, there is a demand for safer products with less irritation to the skin. In particular, recently, awareness of health and attention to natural materials are increasing.
In order to solve these problems, many cosmetic agents have been proposed. For example, cosmetics containing arbutin as an active ingredient and cosmetics using an antioxidant such as α-tocopherol are known. (For example, see Patent Document 1)

JP 60-56912 A

However, various problems remain in these cosmetics, and there is a problem in terms of practicality. For example, arbutin as a whitening ingredient has a drawback of strong bleaching effect but strong cytotoxicity, and α-tocopherol as a scavenger for reactive oxygen species is unstable to light and heat, and has no action / effect. It was enough.
The present invention is characterized in that the above-described problems are solved and a more excellent whitening cosmetic is provided.

The inventors of the present application have continued research on the effective use of extracts derived from natural products, paying attention to bamboo vinegar liquid collected by cooling the steam generated in the process of heating bamboo, The present invention has been accomplished through extensive research on the effect of inhibiting melanin production on cells. That is, the composition of the present invention is composed of 4-ethyl-2-methoxyphenol (A), 2,6-dimethoxyphenol (B), phenylpropanoid glycoside compound (C) among the melanin production-suppressing components in bamboo vinegar. ) Or a combination of (A) and (B) or (A) and (C), or (B) and (C).
These chemical formulas are represented by the following formulas.

  The cosmetic material in the components of the bamboo vinegar solution of the present invention produces a useful whitening effect that is better than commercially available arbutin and that does not exhibit cytotoxicity.

    As the cosmetic material according to the present invention, a plant belonging to the Gramineae bamboo subfamily (common name bamboo, hereinafter simply referred to as bamboo) is used. There are no particular limitations on the type of bamboo that can be used, but Phyllostachys heterocycla is particularly preferred.

  Others, Kurochiku (Phyllostachys nigra var.henosis), Mushroom (Pylylachachys bambusoides), Hotetiku (Phyllostachys bambusoides var.aurea), Medak (Pleioblastus simonii), Yashike (pseudo), Pseudo (pseudo) Bambusa multiplex, Chimonobaambusa markinal, Chimonobaambusa marmoreal, Chimonobaambusa quadrangularis, Narihirada (Semiarundinaria fastuosa), Sinobambusa tousa, isa Bambusa oldhamii), Machiku (Dendrocalamus latifolius) and the like. Examples of methods for collecting bamboo vinegar from these bamboos include, for example, is there

In the present invention, all the above-ground parts and underground parts of bamboo can be used. Specifically, each part such as a bamboo skin (skin) part, a branch part, a leaf part, and a stem part may be used alone or appropriately mixed.

  Moreover, the usage form of bamboo is not particularly limited, and it may be used in the form of powder, plate or bamboo, or may be used as an extract. Moreover, it is also possible to mix and use these.

  The method for extracting the active ingredient from the bamboo vinegar is not particularly limited, and any organic solvent such as ethyl acetate, or a mixture thereof can be suitably used as long as the active ingredient of the bamboo vinegar is suitably extracted. be able to.

  In this invention, the compounding quantity of the active ingredient which consists of at least 1 type chosen from a bamboo vinegar liquid changes with cosmetic agents, However It is good to mix | blend 0.0001-20 weight%. Preferably it is 0.001 to 10 weight%, More preferably, it is good to mix | blend 0.01 to 2 weight%. If the blending amount is less than 0.0001% by weight, the effects of the present invention cannot be exhibited, and some cosmetic agents may be difficult to produce, so it is better not to exceed 20% by weight.

  The test specimen is a bamboo vinegar liquid collected from Misotake by a bamboo vinegar liquid recovery device (Japanese Patent Laid-Open No. 08-157832).

  The bamboo vinegar is extracted with ethyl acetate, and an example is given to explain the present invention in more detail. However, the present invention is not limited to this example.

A mushroom-derived tyrosinase activity inhibition test of 4-ethyl-2-methoxyphenol and 2,6-dimethoxyphenol in bamboo vinegar liquid extracted with ethyl acetate was performed.
The test component concentrations were all 0.033%.
For comparison, comparison was made with arbutin, which is a commercial cosmetic compounding agent.

Test method for inhibition of tyrosinase activity using tyrosine as a substrate 0.1 mL of 3 mM sample solution and 2.8 mL of tyrosine solution were added to a test tube, and preheating was performed for 5 minutes in a constant temperature water bath set at 37 ° C. Next, 0.1 mL of tyrosinase solution (2000 units / mL) was added, and the mixture was heated in a constant temperature water bath for 30 minutes, and the absorbance was measured using a spectrophotometer V-530 (manufactured by JASCO) set to 475 nm. Absorbance was measured by adding dimethyl sulfoxide (hereinafter abbreviated as DMSO) for the blank, and adding sodium phosphate buffer for the color control (sample and blank) instead of the tyrosinase solution. (The absorbance of the sample solution is A, the color control is B, and the blank absorbance and the color control are C and D, respectively.) Inhibition rates are calculated from the average of the three measurement results. Asked.
Tyrosinase activity inhibition rate (%) = [(C−D) − (A−B)] × 100 / (C−D)
Thus, 4-ethyl-2-methoxyphenol showed better inhibitory activity than arbutin. Moreover, since the action is expressed only under conditions using tyrosine as a substrate, it is considered that tyrosinase acts on the component between tyrosine and DOPA, affects the oxidation of tyrosine, and suppresses melanin production. .

DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging activity test of 4-ethyl-2-methoxyphenol and 2,6-dimethoxyphenol in bamboo vinegar extracted with ethyl acetate was performed.
For comparison, comparison was made with α-tocopherol, which is a commercial cosmetic compounding agent.

A 1 mM ethanol solution of a DPPH radical scavenging activity test method sample was prepared, and a dilution series (100 μL each) having a minimum concentration of 5 was prepared in advance on a 96-well microplate, and 100 μL ethanol was added to the dilution series. Then, 50 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazine (hereinafter abbreviated as DPPH) radical ethanol solution was added. After reacting at 25 ° C. for 30 minutes, absorbance was measured using a microplate reader MTP-300 (manufactured by CORONA) set to 517 nm. In the blank test, ethanol was used in place of the sample solution, and the same operation was performed to measure the absorbance (A is the absorbance of the sample solution, B is the absorbance of the blank, and C is the absorbance of the color control). The DPPH radical scavenging rate was determined according to the following formula. Further, based on this result, SC50, which is a 50% erase density, was calculated.
DPPH radical scavenging rate (%) = [1− (A−C) / B] × 100
Also in the DPPH radical scavenging activity test, 4-ethyl-2-methoxyphenol (A), 2,6-dimethoxyphenol (B) and (A) + (B) showed high scavenging activity.

In order to examine 4-ethyl-2-methoxyphenol, 2,6-dimethoxyphenol, and phenylpropanoid glycosides in bamboo vinegar extracted with ethyl acetate, the melanin production inhibitory effect at the cellular level closer to the living body, B16 melanoma cultured cells (in vivo) were used to suppress melanin production.
For comparison, comparison was made with arbutin, which is a commercial cosmetic compounding agent.

B16 mouse melanoma tyrosinase activity inhibition test method (melanin production inhibition test)
Cells having a concentration of 3.0 × 10 4 cells / mL per well of a 24-well plate were seeded and cultured for 24 hours. The medium was replaced with a medium in which 10 μL of a DMSO mixed solution in which a sample adjusted to a predetermined concentration was added to 990 μL of a medium containing theophylline (0.09 mg / mL), and cultured in a CO 2 incubator for 3 days. Thereafter, the medium was removed, the melanin produced using 3N NaOH aqueous solution was dissolved, and the absorbance (A) was measured with a microplate reader set to 405 nm. On the other hand, DMSO was used instead of the sample as a blank test, and the obtained absorbance (B) was used, and the melanin production inhibition rate was calculated by the following formula.
Melanin production inhibition rate (%) = (B−A) × 100 / B

  4-ethyl-2-methoxyphenol, 2,6-dimethoxyphenol and phenylpropanoid glycosides in bamboo vinegar were tested for cytotoxicity by the MTT method. As a result, no value showing cytotoxicity was observed even at a concentration of 0.033 wt% that exhibited a melanin production inhibitory effect.

Cytotoxicity test (MTT method)
Cells with a concentration of 1.0 × 10 4 cells / 90 μL per well of a 96-well plate were seeded and cultured in a CO 2 incubator for 24 hours. Subsequently, 10 μL of a DMSO mixed solution in which a sample having a predetermined concentration was dissolved was added and cultured in a CO 2 incubator for 48 hours. Thereafter, 10 μL (MTT 5 mg / mL) of a mixed solution of MTT (tetrazolium salt) previously adjusted with a phosphate buffer was added, and further incubated for 6 hours. Thereafter, the MTT solution was removed by suction, and MTT formazan produced using 200 μL of acidic isopropanol (adjusted with 1/100 volume of 4N hydrochloric acid aqueous solution with respect to alcohol) was stirred for 10 minutes with a microplate mixer, Absorbance (A) was measured with a microplate reader set at 560 nm. On the other hand, DMSO was added instead of the sample as a blank test, and the absorbance (B) was measured. The cell damage rate was calculated from the following formula.
Cell damage rate (%) = (B−A) × 100 / B

The present invention relates to a cosmetic, and more particularly to an unprecedented cosmetic containing an active ingredient in bamboo vinegar.

Claims (3)

  Among melanin production inhibitory components in bamboo vinegar, contains one of 4-ethyl-2-methoxyphenol (A), 2,6-dimethoxyphenol (B), and phenylpropanoid glycoside compound (C) Cosmetics characterized by.   A cosmetic comprising (A) and (B) or (A) and (C), or (B) and (C) among melanin production-suppressing components contained in bamboo vinegar. A cosmetic comprising (A), (B), and (C) among melanin production-suppressing components contained in bamboo vinegar.