JP2007051979A - Periodontic disease diagnosing instrument and detection method of periodontic disease causing bacteria - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a detection method of periodontic disease causing bacteria, capable of specifying the kinds of causing bacteria of the periodontic disease and capable of being performed in an extremely simple manner, and to provide a periodontic disease diagnostic instrument, capable of being used in the diagnosis of periodontic disease. <P>SOLUTION: The periodontic disease diagnosing instrument is constituted by providing a specimen-receiving part (1), a periodontic disease causing bacteria labelling part (2) for holding an antibody A, having a label provided with respect to the periodontic disease causing bacteria, and held so as to be moved with the movement of the moisture in the specimen to be examined and a periodontic disease causing bacteria detecting part (3), in the order of (1) to (3) where the antibody B to the periodontic disease causing bacteria is fixed. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a tool for diagnosing periodontal disease and a method for detecting periodontal disease-causing bacteria by immunochromatography.

  Periodontal disease is a general term for diseases in periodontal tissue, which is a tissue for supporting teeth, and is mainly caused by infection with specific bacteria. This periodontal disease begins with inflammation of the gingiva, collapses the periodontal tissue, and eventually leads to tooth loss. As described above, periodontal disease has a serious effect on teeth, but it progresses slowly and there is almost no pain, so it worsens without subjective symptoms. Therefore, conventionally, various techniques for diagnosing periodontal diseases have been developed.

  For example, Patent Document 1 discloses periodontal disease (teeth) in which a monoclonal antibody against Bacteroides gingivalis (porphyromonas gingivalis, hereinafter sometimes referred to as “P. gingivalis”), which causes periodontal disease, is reacted with a test sample. (Periodical disease) diagnosis method is described. Patent Document 2 discloses an antibody that specifically reacts with a polysaccharide derived from the bacterial cell surface of periodontal disease-causing bacteria, and a method for diagnosing periodontal disease using the antibody. In these patent documents, several kinds of methods are exemplified as measurement methods used in each diagnostic method, but only the enzyme immunoassay (ELISA) is specifically used in the examples. However, this enzyme immunoassay method is very complicated in the same manner as the other exemplified methods, and it is not easy to carry out the measurement because it requires expertise.

  On the other hand, Patent Document 3 describes a granulocyte elastase measuring reagent as an immunochromatographic elastase reagent, which is useful for early diagnosis of periodontal disease. Unlike the enzyme immunoassay method, the immunochromatography method using this measurement reagent is simple and can be carried out without requiring special knowledge. However, since granulocyte elastase is measured in this technology, elastase induced by bacterial infection that does not cause periodontal disease is detected, and even if it is not affected by periodontal disease, it is positive May be judged. Above all, there is a problem that the technique cannot identify the types of periodontal disease-causing bacteria.

In other words, as described in Non-Patent Document 1, various bacteria causing periodontal diseases are known, but the degree of association between these bacteria and periodontal diseases is also various. Therefore, if the type of bacteria contained in plaque (dental plaque) can be specified, it can be important information regarding treatment of periodontal disease, but the technology of Patent Document 3 cannot obtain such information.
JP-A-60-73463 (Claim 1, page 3, lower left column) JP-A-6-43166 (Claims, paragraph [0019]) JP 2000-39435 A (Claims 1 and 11) Socransky SS et al., “Microbial complexes in subgingival plaque”, Journal of Clinical Periodontology, Vol. 25, pp. 134-144 (1998). Year)

  As described above, a periodontal disease diagnosis method using a monoclonal antibody against a specific periodontal disease-causing bacterium has been known, but it is not as simple as having specialized knowledge. On the other hand, there was a periodontal disease diagnosis method using a very simple technique called immunochromatography, but the type of the causative bacteria could not be identified.

  Thus, the problem to be solved by the present invention is a method for detecting periodontal disease-causing bacteria that can be specified up to the kind of periodontal disease-causing bacteria and can be carried out very easily, and can be used in the method, An object of the present invention is to provide a periodontal disease diagnostic tool that can be used for diagnosis of periodontal disease activity, disease severity, disease risk, and the like.

  In order to solve the above-mentioned problems, the present inventor has conducted extensive research on the configuration of a tool for diagnosing periodontal diseases. As a result, the present invention was completed by finding that the above-mentioned problems can be solved by performing an immunochromatography method using two types of antibodies that specifically react with the target periodontal disease-causing bacteria.

That is, the periodontal disease diagnostic tool of the present invention,
On the water-absorbing substrate,
(1) Test specimen receiver
(2) Periodontal disease-causing bacteria labeling part, which is held against the periodontal disease-causing bacteria and has antibody A so that it can move with the movement of moisture in the test sample; ) Periodontal disease-causing bacteria detector, to which antibody B against periodontal disease-causing bacteria is fixed,
In the order of (1) to (3).

  In the periodontal disease diagnostic tool, (4) a confirmation unit for confirming that water in the test sample has passed the periodontal disease-causing bacteria detection unit is provided at a position following (3). Is preferred. This is because even when the target periodontal disease-causing bacteria is not present in the test sample, it can be easily confirmed that the test has been completed.

  A water-absorbing substrate containing a nonionic surfactant is suitable. This is because the test sample can be rapidly developed and the diagnosis time can be shortened.

  In the periodontal disease diagnostic tool, the antibody A and the antibody B specifically bind to membrane proteins, surface polysaccharides, surface lipids, surface lipopolysaccharides, secreted substances and their complexes of periodontal disease-causing bacteria. It is preferable to react. This is because the target periodontal disease-causing bacteria can be detected even if the test sample is directly subjected to the test without subjecting it to ultrasonic treatment or the like.

  Antibody A and Antibody B include Porphyromonas gingivalis, Bacteroides forsythus, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Streptococcus sangis, Streptococcus oralis, Aikenella colodense and Actinobacterium acetimidum accinomyces Those that react specifically with one or more periodontal disease-causing bacteria selected from the group are preferred. These bacteria are highly correlated with periodontal disease activity, disease severity, disease risk, etc., depending on their abundance, combination of species, immune type in the same species, type of antigen, etc. This is because confirming the presence or absence of these bacteria in the oral cavity is extremely important in the diagnosis of periodontal diseases.

  As said antibody A and antibody B, 1 type, or 2 or more types of monoclonal antibodies are suitable. This is because the bacteria causing periodontal disease can be identified more accurately.

  The method for detecting periodontal disease-causing bacteria according to the present invention is a method for detecting periodontal disease-causing bacteria by an immunochromatography method, which is against periodontal disease-causing bacteria and has a label, and periodontal disease It is characterized by using antibody B against causative bacteria.

  According to the present invention, it is possible to detect a specific type of bacteria that cause periodontal disease from a test sample. Moreover, the operation is extremely simple, and for example, even if the dentist has no knowledge of specialized biochemistry, the presence or absence of specific bacteria can be easily determined. Therefore, the present invention is very useful for facilitating the diagnosis of periodontal diseases in which many people are unaware and can progress.

The periodontal disease diagnostic tool of the present invention,
On the water-absorbing substrate,
(1) Test specimen receiver
(2) Periodontal disease-causing bacteria labeling part, which is held against the periodontal disease-causing bacteria and has antibody A so that it can move with the movement of moisture in the test sample; ) Periodontal disease-causing bacteria detector, to which antibody B against periodontal disease-causing bacteria is fixed,
In the order of (1) to (3).

  The water-absorbing substrate used in the present invention may be any material that can absorb and expand moisture contained in the test sample by capillary action. Although the material in particular is not ask | required, a nylon porous sheet, glass fiber filter paper, a woven fabric, a nonwoven fabric, a filter paper etc. can be used, for example. Moreover, you may combine both by using another base material by a periodontal disease cause bacteria label | marker part, a periodontal disease cause bacteria detection part, etc. For example, the antibody can be easily fixed to a sheet by applying an aqueous solution or dispersion of the antibody to the porous sheet of nitrocellulose and drying it, so that it is useful as a periodontal disease-causing bacteria detection unit. On the other hand, the nitrocellulose porous sheet is not suitable because it is necessary to simply retain the antibody in the periodontal disease-causing bacteria labeling portion without immobilizing the antibody.

  The shape of the water-absorbing substrate is not particularly limited, but if the water-absorbing substrate is too large for the test sample, the moisture may not be sufficiently developed, whereas if it is too small, the determination may be difficult. Therefore, generally, the length of the entire tool in the long axis direction is 40 to 70 mm, the length of the tool in the long axis direction of the periodontal disease-causing bacteria marker is 5 to 12 mm, and the periodontal disease-causing bacteria detection unit is a confirmation unit described later The same length can be set to 17 to 30 mm, the width can be set to 3 to 12 mm, and the thickness can be set to 0.1 to 3 mm. However, these lengths and the like can be appropriately adjusted according to the purpose and application, and are not limited to the exemplified sizes.

The water-absorbing substrate preferably contains a nonionic surfactant. This is because the test sample can be quickly developed and diagnosed more quickly. Examples of the nonionic surfactant that can be used here include polyoxyethylene fatty acid esters such as polyoxyethylene sorbitan fatty acid esters represented by polyoxyethylene sorbitan monolaurate, polyoxyethylene hydrogenated castor oil, and lauric acid monoester. Ethanolamide, myristic acid monoethanolamide, polyoxyethylene higher alcohol ether, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene polyoxypropylene fatty acid ester alkyl glycoside (eg alkyl chain: about C _8-16 ), polyglycerin fatty acid esters (e.g. fatty acid moiety of the alkyl chain: about C _8-16), sucrose fatty acid esters (e.g. fatty acid moiety of the alkyl chain: about C _8-16), etc. can be exemplified, among the polyoxyethylene Polyoxyethylene fatty acid esters such as polyoxyethylene sorbitan fatty acid esters typified by Nso sorbitan monolaurate is preferred.

  In order to include the nonionic surfactant in the water-absorbing substrate, the water-absorbing substrate may be dipped in an aqueous solution of the nonionic surfactant and dried. In this case, the concentration of the nonionic surfactant aqueous solution is preferably 1% by mass or more. If it is less than 1% by mass, the effect of the nonionic surfactant may not be sufficiently exhibited.

  In order to improve the operability, the water-absorbing substrate can be exposed to the outside by exposing the test sample receiving part and exposing the periodontal disease-causing bacteria detecting part to the outside, or by a plastic case or the like. It may be coated.

  The periodontal disease diagnostic tool of the present invention has a test sample receiving part on the water-absorbing base material, but this test sample receiving part does not need to be specially treated and is only one end of the water-absorbing base material. Good. However, a mark may be provided as a portion where the test sample is dropped or immersed in the test sample.

  As shown in FIG. 1, the periodontal disease diagnostic tool of the present invention has a periodontium inside the test sample receiving part 2 (one end of the water-absorbing substrate 1) (on the opposite side to the test sample receiving part). It has a disease-causing bacterium marker 3. The periodontal disease-causing bacteria labeling section 3 holds an antibody A31 that is against the periodontal disease-causing bacteria and has a label so that it can move with the movement of moisture in the test sample.

  An antibody against periodontal disease-causing bacteria to be detected can be prepared by a conventional method such as a hemolytic plaque method.

  A monoclonal antibody is suitable as the antibody. This is because the bacteria causing periodontal disease can be identified more accurately. Monoclonal antibodies can be prepared according to conventional methods. For example, among bacteria causing periodontal disease, a protein or the like peculiar to the bacteria or bacteria group to be detected is isolated and purified, and the mouse is used to immunize the spleen cells, antibody-producing cells, myeloma cells, To obtain a hybridoma. The hybridoma is cloned, and a clone producing an antibody that specifically reacts with the protein or the like is screened. This clone may be cultured and the secreted monoclonal antibody may be purified.

  The antibody of the present invention specifically reacts with one or more of membrane proteins, surface polysaccharides, surface lipids, surface lipopolysaccharides, secreted substances, and complexes of periodontal disease-causing bacteria. Preferably, those that specifically react with one or more of membrane proteins, surface lipopolysaccharides, and complexes thereof, more preferably those that specifically react with one or more of the membrane proteins . In the case of an antibody that reacts specifically with proteins, etc. present in the cytoplasm of periodontal disease-causing bacteria, the bacteria must be destroyed by sonicating the test sample. This is because if used, the test sample can be directly subjected to the test.

  The periodontal disease-causing bacteria to which the antibody of the present invention specifically binds are preferably those having a deeper relationship with periodontal diseases. This is to make the diagnosis of periodontal disease more accurate. Such periodontal disease-causing bacteria include P.I. Gindivaris (Porphyromonas gingivalis), Bacteroides forsythus (Treponema denticola), Fusobacterium nucleatum (Fusobacterium nucleatum), Prevotella intermedia (Prevotella intermedia), Streptococcus Examples include Eikenella corrodens, Actinobacillus actinomycetemcomitans, and Actinomyces naeslundii.

  Examples of the periodontal disease-causing bacteria to be detected include P.I. More preferably, one or more selected from the group consisting of Gingivalis, Bacteroides forsythus, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Aikenella collodense, Actinobacillus actinomycetemcomitans and Actinomyces nesrandi. 1 or 2 or more selected from the group consisting of Gingivalis, Bacteroides forsythus, Actinobathiras Actinomycetemcomitans and Treponema denticola is more preferred. Gingivalis is particularly preferred.

  The obtained antibody needs to be labeled for detection of periodontal disease-causing bacteria. The antibody labeling method may be a known one. For example, the antibody is added to a solution of metal colloid particles such as gold colloid particles or synthetic rubber resin particles such as latex particles, and this antibody is applied to a water-absorbing substrate. It can be labeled by soaking, air drying or freeze drying.

  As a method for holding antibody A, which is against periodontal disease-causing bacteria and has a label, on the hygroscopic substrate so that it can be moved along with the movement of moisture in the test sample, a conventional method can be used. . For example, antibody A may be dispersed in an aqueous solution of a water-soluble polymer or saccharide, absorbed at a predetermined position, and then dried. Examples of the water-soluble polymer used here include polyvinyl alcohol, and examples of the saccharide include sucrose. Furthermore, in order to facilitate the movement of antibody A, a surfactant solution may be applied in advance to the planned site of the periodontal disease-causing bacterial marker, and then a dispersion of antibody A may be applied and dried. Good.

  In the periodontal disease diagnostic tool of the present invention, the periodontal disease-causing bacteria is detected at a position following the periodontal disease-causing bacteria labeling unit 3, that is, on the side opposite to the test sample receiving unit 2 of the periodontal disease-causing bacteria labeling unit 3. Part 4 is provided. The periodontal disease-causing bacteria detection unit 4 is fixed with an antibody B41 against periodontal disease-causing bacteria.

  Antibody B can be prepared by the same method as antibody A, and should bind to the same periodontal disease-causing bacteria as antibody A. This is because it is necessary to bind to the periodontal disease-causing bacteria bound to the antibody A or a fragment thereof. The binding target of antibody B may be the same as that of antibody A, but it is preferable to bind to a binding target different from antibody A. This is because the detection sensitivity of periodontal disease-causing bacteria can be further improved.

  The method for immobilizing antibody B is not particularly limited, and a method corresponding to the type of the water-absorbing substrate to be used can be employed. For example, in the case of using a nitrocellulose substrate as the water-absorbing substrate, it can be fixed simply by applying an aqueous solution or dispersion of antibody B and then drying. Here, “fixation” refers to the degree to which antibody B does not move with the movement of moisture contained in the test sample.

  In the periodontal disease diagnostic tool of the present invention, the position following the periodontal disease-causing bacteria detection unit 4, that is, on the side opposite to the test sample receiving unit 2 of the periodontal disease-causing bacteria detection unit 4, It is preferable to provide a confirmation unit 5 for confirming that water has passed through the periodontal disease-causing bacteria detection unit. This is because it is possible to know the end of the test even if the test sample does not contain periodontal disease-causing bacteria and the periodontal disease-causing bacteria detection unit does not change.

  As an aspect of the confirmation unit of the present invention, a line of a water-soluble dye may be provided at a predetermined position. As a result, when the water in the test sample passes through the confirmation part, the water-soluble dye dissolves and moves with the development of the water, so even if there is no change in the periodontal disease-causing bacteria detection part, This is because it can be confirmed that the test sample has passed through the periodontal disease-causing bacteria labeling part and the periodontal disease-causing bacteria detection part.

  As a method for providing the confirmation unit, an aqueous solution of a water-soluble dye may be applied at a predetermined position and dried. As the water-soluble dye, bromophenol blue or the like can be used.

  The method for detecting periodontal disease-causing bacteria according to the present invention is a method for detecting periodontal disease-causing bacteria by an immunochromatography method, which is against periodontal disease-causing bacteria and has a label, and periodontal disease It is characterized by using antibody B against causative bacteria. Since the method can be performed using the periodontal disease diagnostic tool of the present invention described above, the method will be described below with reference to FIG. 1 schematically showing the periodontal disease diagnostic tool of the present invention.

  Test samples include saliva, mouthwash discharge, suspension of gingival and subgingival plaque, gingival crevice, which is considered to contain periodontal disease-causing bacteria when the subject suffers from periodontal disease. Exudate can be mentioned. If the test sample contains a small amount of water, purified water or distilled water may be added.

  When the periodontal disease diagnostic tool of the present invention is used, an appropriate amount of the test sample is dropped on the test sample receiving part 2 in FIG. 1, or the test sample receiving part is immersed in the test sample. Moisture contained in the test sample is developed toward the other end side by capillary action, and bacteria and the like contained in the test sample move accordingly.

  When a periodontal disease-causing bacterium that specifically reacts with an antibody is present in the test sample, the periodontal disease-causing bacterium binds to the antibody A31 at the periodontal disease-causing bacteria labeling unit 3. The conjugate of the periodontal disease-causing bacterial marker 3 and antibody A31 further moves with the development of moisture. In the periodontal disease-causing bacteria detecting unit 4, the periodontal disease-causing bacteria that are bound to the antibody A31 are further bound to the antibody B41. Since this antibody B41 is fixed to the base material 1, periodontal disease-causing bacteria cannot move any further. As a result, since the label on the antibody A bound to the periodontal disease-causing bacteria is concentrated at this site, the periodontal disease-causing bacteria detection unit 4 develops color or the like.

  Thereafter, when the moisture in the test sample further develops and reaches the confirmation unit 5, a change appears in the confirmation unit 5. For example, when a water-soluble marker is used, since the water-soluble marker dissolves in water and moves as the water moves, it can be confirmed that the water has reached the confirmation unit 5. Therefore, even if there is no change in the periodontal disease-causing bacteria detection unit 4 without any predetermined periodontal disease-causing bacteria in the test sample, the test is completed if the confirmation unit 5 changes. You can know what you did.

  As described above, according to the present invention, a specific periodontal disease-causing bacterium can be detected very easily. Therefore, the periodontal disease diagnostic tool and the periodontal disease-causing bacterium detection method according to the present invention are very useful. It is.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited by the following examples, but may be appropriately modified within a range that can meet the purpose described above and below. It is also possible to implement, and they are all included in the technical scope of the present invention.

Production Example 1-1 Preparation of Monoclonal Antibody A monoclonal antibody against Pase-C, one of trypsin-like enzymes produced by Porphyromonas gingivalis, one of the bacteria having a very strong relationship with periodontal diseases, was prepared. Specifically, Pase-C was first purified and mice were immunized. Spleen cells were obtained from the mouse, and hybridomas were obtained by fusing the spleen cells and myeloma according to a conventional method. This hybridoma is seeded in a 96-well multiplate so that the number of cells per well is one, and the culture supernatant of the well in which the formation of colonies has been observed is collected and reacted with the Pase-C crude extract. Screening was conducted by examining sex. After recloning, a hybridoma that is an antibody-producing strain having a strong reactivity with the antigen was obtained. In order to select a clone with stronger and more stable reactivity, the hybridoma was recloned. A crude extract of Pase-C was used as an antigen, and P.P. An enzyme immunoassay is performed using the purified enzyme Pase-S of Gindivarius 381 as a negative antigen control, and a hybridoma that is a particularly reactive subclone is selected as a hybridoma for production of a monoclonal antibody, and this hybridoma is produced. Mouse ascites containing anti-Pase-C monoclonal antibody was obtained. The mouse ascites was filtered and inactivated at 56 ° C. for 30 minutes, and this inactivated was designated as an anti-Pase-C monoclonal antibody. The antibody was labeled by immersing it in a solution of colloidal gold particles. Separately, P.A. A monoclonal antibody against Gingivalis was prepared, which was not labeled.

Production Example 1-2 Preparation of Periodontal Disease Diagnosis Tool According to the Present Invention A sheet made of glass fiber filter paper is cut into a length of 10 mm, and an aqueous solution of gold colloid particle-labeled antibody obtained in Production Example 1-1 is applied. And dried at 50 ° C. all day and night. This was used as a periodontal disease-causing bacterial marker.

  Moreover, the sheet | seat which consists of nitrocellulose was cut | judged to 25 mm in length, the aqueous solution of the monoclonal antibody (no label | marker) obtained by manufacture example 1-1 was apply | coated to the predetermined position, and it was made to dry naturally. After drying, the product was dipped in an aqueous solution containing 10% of a milk-derived protein preparation and allowed to dry naturally to obtain a periodontal disease-causing bacteria detection unit. Further, a bromophenol blue water-soluble dye was applied to a predetermined position on the same sheet, followed by natural drying, which was used as a confirmation part.

  Separately, a sheet made of polyester is cut into a length of 15 mm, a 20 mM Tris buffer solution containing 1% polyoxyethylene sorbitan monolaurate is applied, and dried at 25 ° C. all day and night. Pad). Moreover, what cut | disconnected the sheet | seat which consists of cellulose to length 20mm was used as the test sample absorption pad after completion | finish of a test | inspection.

  A plastic sheet having a surface coated with an adhesive was cut to a length of 60 mm and used as a base sheet. A nitrocellulose sheet having a periodontal disease-causing bacteria detection part and a confirmation part was attached to the center part of the base sheet. Paste the glass fiber filter paper sheet with the periodontal disease-causing bacteria labeling part to the end of the periodontal disease-causing bacteria detection part side so that the end part overlaps with this nitrocellulose sheet, and after the completion of the inspection to the end on the confirmation part side A test sample absorption pad was attached. Furthermore, a polyester sheet having a test sample receiving part was stuck on the base sheet so that the end overlapped with the glass fiber filter paper sheet having the periodontal disease-causing bacteria labeling part. Lastly, an adhesive lamination film made of polyethylene terephthalate is cut to a length of 30 mm, and the film is covered with a periodontal disease-causing bacteria detection part and a part of the test sample absorption pad after completion of the test. It was pasted as if The obtained sheet was adjusted to a width of 8 mm to obtain a periodontal disease diagnostic tool according to the present invention.

Test example 1
The periodontal disease diagnostic tool obtained in Production Example 1-2 was tested for specificity against bacterial species. That is, a bacterial suspension was prepared so that each bacterial strain shown in Table 1 had a bacterial number concentration of 10 ⁷ cells / mL, and the bacterial suspension was used to test the periodontal disease diagnostic tool of Production Examples 1 to 3 above. An appropriate amount was dropped on the sample receiving part, and the determination was made 15 minutes, 30 minutes and 60 minutes after dropping. The results are shown in Table 1.

  As the result, P.I. By using the periodontal disease diagnostic tool of the present invention having a monoclonal antibody against gingivalis, P. p. Gingivalis could be detected very simply and specifically.

Test example 2
The water-absorbent substrate sheet used in Production Example 1-3 was immersed in a 0, 1, 3, 5 or 10% by mass aqueous solution of Tween 20 and dried naturally. In addition, a mouthwash discharge solution was collected from a specific subject (age: 48 years old, sex: male) three times a day (10 o'clock, 14:00 and 17:00) for 3 days, and one end of the water-absorbent substrate sheet was collected. An appropriate amount was dropped on the sample, and the development of the sample after 10 minutes and 30 minutes was observed. ○ when the moisture spreads very quickly to the other end (the end opposite to the end where the sample is dropped), △ when the moisture spreads to the other end, but slightly slower, and the spreading speed is slow The results are shown in Table 2, where x indicates that the other end cannot be fully developed.

  As the result, even when the nonionic surfactant is not contained in the water-absorbing substrate, the moisture contained in the sample can be developed. However, it has been demonstrated that the development of moisture can be made very rapid by adding a nonionic surfactant to the substrate.

It is a figure which shows typically an example of the periodontal disease diagnostic tool of this invention.

Explanation of symbols

1: Water-absorbing base material 2: Test specimen receiving part 3: Periodontal disease-causing bacterial labeling part 31: Antibody A
4: Periodontal disease-causing bacteria detection part 41: Antibody B
5: Confirmation part

Claims (8)

A tool for diagnosing periodontal disease,
On the water-absorbing substrate,
(1) Test specimen receiver
(2) Periodontal disease-causing bacteria labeling part, which is held against the periodontal disease-causing bacteria and has antibody A so that it can move with the movement of moisture in the test sample; ) Periodontal disease-causing bacteria detector, to which antibody B against periodontal disease-causing bacteria is fixed,
In the order of (1) to (3).   Furthermore, (4) The periodontal disease diagnostic tool of Claim 1 which has a confirmation part for confirming that the water | moisture content in a test sample passed the said periodontal disease cause bacteria detection part.   The periodontal disease diagnostic tool according to claim 1 or 2, wherein the water-absorbing substrate contains a nonionic surfactant.   Antibody A and antibody B specifically react with one or more of membrane proteins, surface polysaccharides, surface lipids, surface lipopolysaccharides, secreted substances and complexes of periodontal disease-causing bacteria The periodontal disease diagnostic tool according to any one of claims 1 to 3.   Antibody A and Antibody B are Porphyromonas gingivalis, Bacteroides forsythus, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Streptococcus sangis, Streptococcus oralis, Aikenella colodens, Actinobacteriacem The periodontal disease diagnostic tool according to any one of claims 1 to 4, which specifically reacts with one or more periodontal disease-causing bacteria selected from the group.   The periodontal disease diagnostic tool according to any one of claims 1 to 5, wherein the antibody A is one or more monoclonal antibodies.   The periodontal disease diagnostic tool according to any one of claims 1 to 6, wherein the antibody B is one or more monoclonal antibodies. A method of detecting periodontal disease-causing bacteria by immunochromatography,
A method for detecting a periodontal disease-causing bacterium, comprising using an antibody A against a periodontal disease-causing bacterium and having a label, and an antibody B against the periodontal disease-causing bacterium.

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