JP2007043940A - Method for collecting inositol - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for easily collecting inositol at a low cost by utilizing a production process of beet sugar. <P>SOLUTION: The method for collecting the inositol can collect the inositol by crystallizing the inositol contained in a liquid after being passed through an ion-exchange resin by a simple operation of removing betaine and other materials preventing the collection from waste molasses by the adsorption by the ion exchange resin when collecting the inositol from the waste molasses. The betaine can be recovered by eluting the betaine adsorbed on the ion-exchange resin by utilizing the inositol-collecting process of the method. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method for recovering inositol, and more particularly to a method for recovering inositol from molasses produced in beet sugar production.

  Inositol, which is a water-soluble vitamin-like substance, is widely distributed in nature, and is known to be present in extracellular supporting substances such as higher plants, especially cereals and seeds. In rice bran, it is present in large quantities in the form of calcium and magnesium mixed salts (phytin) of phytic acid. There are many unexplained parts about the physiological activity of inositol, and it has recently been elucidated by the present inventors that it has a function of increasing the plasmalogen concentration in vivo. Thus, inositol, whose physiological activity is being elucidated, is expected to be used as a food having a health function with the purpose of maintaining health and preventing disease and as a pharmaceutical product.

  Various methods for producing inositol have been reported. Since rice bran is present in large quantities in rice bran, there is a method of producing (extracting) rice bran collected from the production area as a raw material by degreasing, extracting and enzymatic reaction.

  It is known that inositol is also contained in sugar beet, which is a raw material for sucrose production. However, since the main purpose of sugarcane production process from sugar beet (hereinafter referred to as beet sugar manufacturing process) is mainly for the recovery of sugar, inositol is transferred to molasses (hereinafter referred to as waste molasses) after producing beet sugar. To do. However, since the molasses contains a large amount of saccharides and other substances, it has been difficult to separate inositol with a low content from the molasses.

Among them, several methods have been reported as methods for producing (recovering) inositol using sugar beet sugar liquid (waste molasses) as a raw material (see, for example, Patent Documents 1 to 3). In the methods of Patent Documents 1 and 2, galactosinol in sugar beet molasses is decomposed with α-galactosidase to convert it into inositol, and the content (purity) of inositol is improved, followed by chromatographic separation. This is a method in which yeast (yeast) is inoculated and cultured to remove unnecessary sugars, and then the culture solution is filtered and concentrated to separate and collect inositol. In the method of Patent Document 3, beet sugar solution is aerated with yeast, and sugars such as sucrose, fructose, and glucose are assimilated while remaining inositol to reduce sugar as much as possible, and the content of inositol ( Purity). Next, the inositol-containing liquid is further passed through a chromatographic tower of a salt-type cation exchange resin, and the inositol fraction flowing out is separated and collected by chromatography to obtain a highly pure inositol liquid. Then, this inositol fraction is concentrated and cooled to precipitate inositol crystals.
Japanese Patent Publication No. 48-2356 (announced on January 24, 1973) Japanese Patent Publication No.53-28993 (announced August 17, 1978) JP 2000-236890 A (published September 5, 2000)

  However, in the inositol production (recovery) methods described in Patent Documents 1 to 3, it is necessary to extract inositol through the above-described many steps, and the operation is complicated. Therefore, there is a problem that the cost is high although the purity of inositol is relatively low. In Patent Document 3, since chromatographic separation is performed after yeast treatment, the operation is simplified compared to the methods of Patent Documents 1 and 2, but in any of the methods of Patent Documents 1 to 3, the chromatography step is performed. Therefore, it can be said that the operation is complicated.

  By the way, it is known that beet sugar solution contains betaine (trimethylglycine) in addition to inositol. Betaine is used as an amino acid-based plant natural moisturizing agent in cosmetic materials and has a use as a seasoning. In recent years, betaine is produced (recovered) using the method of Patent Document 3, for example. In this case, it is necessary to perform chromatographic separation using a simulated moving bed. When betaine is collected together with inositol, the operation becomes more complicated.

  Accordingly, the present invention has been made to solve the above-described problems, and an object of the present invention is to provide a method for easily recovering (manufacturing) inositol at low cost by using a beet sugar manufacturing process. There is to do. In addition, the present invention provides a method that can be easily carried out between inositol recovery steps even when betaine is recovered.

  The inventors of the present application conducted various studies focusing on the recovery of inositol, a by-product obtained in the beet sugar manufacturing process, and found that the sugar and betaine in the beet sugar manufacturing process in recent years have been studied. With the development of recovery technology, we have found that certain by-products (especially inositol) are concentrated in molasses. Specifically, as a result of intensive studies by the inventors of the present application, it has been found that substances that interfere with inositol recovery are decreasing while the molasses is precisely separated and analyzed, and the present invention has been completed.

  That is, the method according to the present invention is a method for recovering inositol from waste molasses produced after collecting beet sugar from sugar beet, and passing the waste molasses through an ion exchange column filled with an ion exchange resin. An ion exchange step of adsorbing a substance different from inositol contained in the waste molasses and recovering the entire amount of liquid after passing through the ion exchange column, and concentrating the recovered liquid to precipitate inositol crystals. And a crystallization step.

  According to said method, inositol can be easily collect | recovered (manufacture) using the manufacturing process of sugar beet sugar, and inositol can be collect | recovered at low cost.

  Specifically, according to the method of the present invention, inositol crystals are precipitated by passing through a column packed with an ion exchange resin in the ion exchange step and concentrating using the total amount of the passed solution. Can do. That is, inositol can be easily recovered from the molasses without using a complicated method such as conventional chromatographic separation.

  That is, as described above, the present inventors have a relatively low content of sucrose and betaine, which are substances that interfere with the recovery of inositol in molasses obtained after collecting beet sugar from sugar beet, and concentrate inositol. It became clear that. Therefore, in the present invention, substances other than inositol (specifically betaine and other substances described later) present in the molasses can be adsorbed and removed by the ion exchange resin. Thereby, inositol can be favorably and easily crystallized by using the recovered liquid recovered after the liquid flow.

  Thus, since inositol can be manufactured from waste molasses using a simple process, the manufacturing cost can be significantly reduced as compared with the conventional manufacturing method. Therefore, inositol can be provided at low cost.

  That is, in the method according to the present invention, it is preferable that in the ion exchange step, the waste molasses is passed through the ion exchange column to adsorb a substance that hinders inositol recovery to the ion exchange resin.

  In the method according to the present invention, it is preferable that the liquid collected by passing through the ion exchange column in the ion exchange step is filtered through diatomaceous earth.

  As a result, it is possible to remove non-ionic fine substances that interfere with the recovery of inositol.

  In the method according to the present invention, in the ion exchange step, the molasses is filled with a cation exchange column filled with a cation exchange resin, and an anion exchange column filled with an anion exchange resin; It is preferable to pass through.

  Specifically, the molasses is preferably passed through a cation exchange column filled with a cation exchange resin and then passed through an anion exchange column filled with an anion exchange resin. .

  In the method according to the present invention, it is preferable that the ion exchange step repeatedly passes through the cation exchange column and the anion exchange column.

  The betaine remaining in the molasses by passing through the cation exchange column and the anion exchange column repeatedly, compared with the case of passing through the cation exchange column and the anion exchange column once each. And other inositol recovery interfering substances can be further reduced. Therefore, inositol can be recovered more efficiently.

  In the method according to the present invention, it is preferable that betaine in the molasses is adsorbed on the cation exchange resin, and the substance different from betaine is adsorbed on the anion exchange resin.

  In the method according to the present invention, the cation exchange resin is preferably passed through a cation exchange column of a porous type and filled with a styrenic strongly acidic cation exchange resin.

  The inventors of the present application have found that betaine adsorbs very well by using a resin having the above characteristics.

  Therefore, inositol can be efficiently recovered by passing waste molasses through a cation exchange column using a resin having the above characteristics.

  As will be described later, when recovering betaine from molasses by recovering betaine adsorbed on a cation exchange column, the recovery of betaine can be achieved by using a resin having the above characteristics. Can be improved.

  That is, in the method according to the present invention, the betaine adsorbed on the cation exchange resin is eluted by passing a predetermined concentration of aqueous sodium hydroxide solution through the cation exchange column after passing the waste molasses. Is preferred. In particular, by using a cation exchange column that is porous as described above and is filled with a styrene-based strongly acidic cation exchange resin, the molasses are allowed to flow, so that the adsorption property of betaine to the resin is high, Therefore, betaine can be efficiently recovered from the resin by passing a predetermined concentration of aqueous sodium hydroxide solution.

  Thus, according to the method of the present invention, betaine can be easily recovered from molasses at a low cost together with inositol.

  Specifically, in the method according to the present invention, it is preferable to pass 0.5N to 1.5N sodium hydroxide aqueous solution.

  Thereby, betaine can be recovered with high purity from a cation exchange column of a porous type and filled with a styrene-based strongly acidic cation exchange resin.

  As described above, the method for recovering inositol according to the present invention is a method for recovering inositol from the molasses produced after collecting sugar beet sugar from sugar beet, which is filled with an ion exchange resin. The ion exchange step of passing through the ion exchange column and adsorbing a substance different from inositol contained in the waste molasses and recovering the entire amount of liquid after passing through the ion exchange column, and the recovered liquid And a crystallization step of concentrating and precipitating inositol crystals.

  Inositol can be crystallized by passing through a column filled with an ion exchange resin in the above column exchange step and concentrating with the total amount of the passed liquid, so that the conventional chromatographic separation can be performed. Inositol can be easily recovered from the molasses without using a complicated method.

  Moreover, since inositol can be manufactured from waste molasses using a simple process, the manufacturing cost can be reduced, so that inositol can be provided at low cost.

  Inositol is a general term for cyclohexane hexahydric alcohol and is a water-soluble vitamin-like substance. Although it is possible to synthesize from glucose 6-phosphate in a living body, since there is a limit to the synthesis, a method of extracting and ingesting from a plant containing a lot of inositol is generally used.

  Therefore, the inventors of the present application focused on the molasses produced in the recent beet sugar production process for the production of inositol, and based on the new knowledge that substances that interfere with the recovery of inositol are decreasing in the molasses, The inventors have found a method for recovering inositol from molasses and a method for recovering betaine, which could not be easily achieved from the prior art, and completed the present invention.

  Below, one Embodiment of the method which collect | recovers inositol from the waste molasses produced after extract | collecting sugar beet sugar from sugar beet based on this invention is described.

  The method according to the present invention is a method for recovering inositol from waste molasses produced after collecting beet sugar from sugar beet, and passing the waste molasses through an ion exchange column filled with an ion exchange resin An ion exchange step of adsorbing a substance different from inositol contained in the molasses and recovering the entire amount of liquid after passing through the ion exchange column, and a crystal for concentrating the recovered liquid to precipitate inositol crystals A process comprising the steps of:

  In the present specification, “waste molasses” is obtained by diluting a stock solution of molasses obtained after collecting beet sugar from sugar beet to a Brix concentration of 5 to 45%, or a stock solution of waste molasses having a Brix concentration of 5 It refers to a crystal prepared by diluting to a Brix concentration of 5 to 45% after being diluted to ˜45% and crystallized by standing at a low temperature of 5 to 8 ° C. In the present specification, “Brix concentration” refers to the percent concentration of soluble solids contained in a sample (aqueous solution). Soluble solids include water, such as sugar, salts, proteins, and acids. All substances are applicable. The solvent used for dilution includes water (demineralized water). The specific composition and concentration (hereinafter referred to as Brix concentration) in the diluted molasses (hereinafter simply referred to as molasses) include raffinose concentration of 0.1 to 0.9% and sucrose concentration of 0. 0.1 to 1.9%, glucose concentration 1.1 to 5.9%, fructose concentration 1.1 to 5.9%, inositol concentration 0.1 to 3.9%, betaine concentration 1.1 to 12.9 % Is included. For example, in the case of diluted molasses with a Brix concentration of 28%, a raffinose concentration of 0.81%, a sucrose concentration of 0.26%, a glucose concentration of 3.92%, a fructose concentration of 4.89%, an inositol concentration of 1.64%, betaine Containing a concentration of 11.78%.

  The stock solution of molasses having such a composition is produced by a beet sugar manufacturing process including an ion chromatography process. In the beet sugar manufacturing process including the ion chromatography process, sucrose can be recovered at a high recovery rate of 93 to 94%.

  The molasses produced by the sugar beet production process as described above is diluted as described above, and then passed through an ion exchange column filled with an ion exchange resin (ion exchange process). The present invention recovers inositol well and easily from waste molasses by adsorbing and removing a substance that hinders recovery when inositol is recovered from waste molasses (hereinafter referred to as inositol recovery interfering substance) to an ion exchange resin. it can. Specifically, a cation exchange column filled with a cation exchange resin and an anion exchange column filled with an anion exchange resin are used as the ion exchange column.

  A cation exchange column packed with a cation exchange resin is used to remove betaine, which is an inositol recovery interfering substance contained in waste molasses, from the molasses. Specifically, the betaine contained in the molasses is removed by adsorbing the cation exchange resin. In the beet sugar manufacturing process described above, the recovery rate of betaine is improved compared to the conventional beet sugar manufacturing process, but cannot be completely recovered. Therefore, betaine is also contained in molasses. If betaine is present in a large amount, it cannot be completely adsorbed in the cation exchange resin process and cannot be separated from inositol. Alternatively, the amount of inositol recovered is very small relative to the amount of processing liquid. Therefore, it is necessary to remove betaine when recovering inositol. The cation exchange resin is preferably a porous type and is a styrene-based strong acid cation exchange resin. Among these, in consideration of the adsorption property of betaine, it is preferable to use a strongly acidic cation exchange resin substituted with Na type as described in Examples described later.

  An anion exchange column packed with an anion exchange resin is used to remove inositol recovery interfering substances other than betaine contained in the molasses. Inositol recovery interfering substances other than betaine include common anionic inorganic substances such as raw sugar beet or soil-derived chloride ions, nitrate ions, sulfate ions, etc., and waste molasses is passed through an anion exchange column. These are adsorbed on the anion exchange resin. By performing the anion exchange resin treatment in this manner, the inositol-containing liquid is purified and the purity is increased, so that the purity of inositol finally obtained is also increased. As the anion exchange resin, a conventionally known anion exchange resin can be used. Among these, in view of the adsorptivity of general anionic inorganic substances such as chloride, nitrate, and sulfate ions derived from raw sugar beet or soil, a weakly basic anion as described in the examples described later. It is preferable to use an ion exchange resin.

  The order of passing the molasses through the cation exchange column and the anion exchange column is not particularly limited, but by passing the molasses through the cation exchange column prior to the anion exchange column, It is considered that a large amount of the cation material can be removed first, and a relatively small amount of the anion material can be effectively removed by the anion exchange column in the subsequent step.

  The molasses may be passed through the cation exchange column and the anion exchange column only once, but as described in the examples described later, the cation exchange column and the anion exchange A configuration in which liquid is repeatedly passed through the column may be employed. By comprising in this way, the betaine and other inositol collection | reduction interference substance which remain | survive in waste molasses can be reduced further compared with the case where it is made to pass through once each. Therefore, more efficient and good inositol crystals can be deposited.

  It is preferable that the total amount of the liquid passed through each ion exchange column is recovered, and then the recovered liquid is filtered through diatomaceous earth. By filtering through diatomaceous earth, it is possible to remove a non-ionic fine substance that interferes with the recovery of inositol. As diatomaceous earth, standard supercell, hyflo supercell, perlite, etc. can be used.

  The liquid after diatomaceous earth filtration is concentrated to promote crystallization of inositol. Concentration is not preferable because it takes time to crystallize if the concentration is low. On the other hand, if the concentration is too high, the clay will rise and the separation operation in the next step (post process) will be difficult. It is preferable to concentrate until it becomes -80. Further, the concentration is preferably performed under reduced pressure, and is preferably concentrated under reduced pressure at 45 to 55 ° C. in order to avoid coloring.

  The concentrated liquid obtained through the ion exchange process described above contains betaine, a substance that interferes with inositol recovery, and common anionic inorganic substances such as chloride, nitrate, and sulfate ions derived from raw sugar beet or soil. It is an inositol fraction that is not contained or is not contained to the extent that hinders the recovery of inositol. By allowing it to stand as it is, inositol crystals can be easily precipitated.

  In the beet sugar manufacturing process described above, the sucrose recovery rate is improved as compared with the conventional beet sugar manufacturing process, but since it cannot be completely recovered, sucrose is also included in the molasses. In the present invention, the operation for removing sucrose is not performed in the ion exchange step described above, but since the sucrose contained in the molasses is very little and the crystallinity of inositol is higher than that of sucrose, If the inositol fraction is allowed to stand as it is, inositol can be crystallized prior to sucrose.

  According to the method of the present invention, inositol crystals having a purity of 85 to 99% can be obtained. Moreover, according to the method of the present invention, inositol can be recovered from the molasses at a recovery rate of 82 to 99% (the amount of inositol in the molasses is 100%).

  As described above, betaine is a substance that interferes with the recovery of inositol. On the other hand, betaine is used as a seasoning in addition to being used as an amino acid-based plant natural moisturizing agent in cosmetic materials. Therefore, in the present invention, the betaine can be recovered.

  As described above, in the present invention, betaine is adsorbed on the cation exchange resin and removed from the molasses. Therefore, betaine can be easily recovered by eluting it from the cation exchange resin. The elution method is not particularly limited as long as a solvent capable of eluting betaine from the cation exchange resin is passed through the cation exchange column. It is preferable to pass a sodium hydroxide aqueous solution. In this case, if the sodium hydroxide concentration is too low, it takes time to elute from the column. If it is too high, a large amount of acid is required for neutralizing the product betaine. It is preferable to use those. By such a method, betaine can be eluted from the cation exchange resin, and since the substance other than betaine is not contained in the recovered liquid after elution, it can be said that this recovered liquid is a betaine fraction.

  Next, the betaine fraction is concentrated. Concentration is preferably performed until the Brix concentration is 65 to 80%. The concentration is preferably performed under reduced pressure, and is preferably performed at 45 to 55 ° C. in order to avoid coloring. Furthermore, crude betaine crystals can be obtained by cooling the concentrated betaine fraction to room temperature and centrifuging (3,000-4000 rpm, 10-20 minutes). The crude crystals are 58-85% pure. Next, in order to obtain a higher-purity betaine crystal, the crude crystal is dissolved in demineralized water and recrystallized. It is preferable that the dissolution has a Brix concentration of 65 to 80%. By this operation, a betaine crystal having a purity of 85 to 98% can be obtained. If the amount of betaine in the molasses is 100%, betaine can be recovered at a recovery rate of 68 to 92% using the above method.

  In addition, if it is those skilled in the art, like the above-mentioned betaine elution method of a cation exchange resin, chlorine ion, nitrate ion, sulfate ion derived from raw sugar beet or soil of the raw material adsorbed to the anion exchange resin of the anion exchange column Elution and recovery of general anionic inorganic substances such as these can be easily achieved.

  According to the method of the present invention described above, inositol crystals can be precipitated by passing the solution through a column filled with an ion exchange resin in the ion exchange step and concentrating using the total amount of the passed solution. . In other words, without using a complicated method such as conventional chromatographic separation, betaine and other substances that interfere with the recovery of inositol in molasses are adsorbed on the ion exchange column and removed from the molasses. Inositol can be recovered from molasses by a simple operation. Therefore, inositol can be easily recovered (manufactured) using a beet sugar manufacturing process, and inositol can be recovered at low cost.

  Moreover, according to the above-described method of the present invention, betaine that has been attracting attention in recent years can be recovered using a simple operation of eluting from an ion exchange column and concentrating.

  In the above method, diluted molasses obtained by diluting a molasses stock solution obtained after collecting beet sugar from sugar beet is passed through a cation exchange column as it is, diluted to a Brix concentration of 5 to 45%. However, the present invention is not limited to this, and after diluting the molasses stock solution to a Brix concentration of 5 to 45%, the diluted molasses is allowed to stand at 5 to 9 ° C. and contains inositol. It is good also as a structure which crystallizes several types of substances, collect | recovers the crystal substance by centrifugation, and lets liquid prepared molasses diluted to the Brix density | concentration 10-35% pass through a cation exchange column. By repeating the crystallization, the non-crystallized product is eliminated and the purity of the crystallized product is increased.

  The invention is illustrated in more detail by the following examples, but should not be limited thereto.

[Example 1]
The present inventors recovered inositol using the molasses produced by the above-described beet sugar production process.

  A flowchart of the inositol recovery method of this example is shown in FIG. As shown in FIG. 1, the molasses stock solution produced by the beet sugar production process is diluted with demineralized water to a Brix concentration of 28%, and 45 L of diluted molasses (raffinose concentration 0.81%, sucrose concentration 0. 26%, glucose concentration 3.92%, fructose concentration 4.89%, inositol concentration 1.64%, betaine concentration 11.78%) (S10 in FIG. 1). Next, the diluted molasses was passed through an ion exchange column (S11).

  Specifically, first, a cation exchange column (Mitsubishi Kasei Co., Ltd., column size inner diameter 25 cm × length 90 cm) formed by packing diluted acidic molasses (45 L) with strongly acidic cation exchange resin PK216 substituted with Na type. , SV2.0) (S11a). Then, after passing through, diluted molasses remaining in the cation exchange column was pushed out with demineralized water, and the whole amount was recovered (S11b).

  Next, an anion exchange column (manufactured by Mitsubishi Kasei Co., Ltd., column size inner diameter 25 cm × length 110 cm, SV2.0 (SV: Space Velocity / (Volume (space) speed: amount of treatment liquid per unit time (h))) (S11c).

  After passing through the anion exchange column, the residual liquid of the anion exchange column is pushed out with demineralized water, and all of these liquids are recovered (S11d), and the entire recovered liquid is used as a standard supercell (Hyflo Supercell / Johnsmanville). ) Was used to filter diatomaceous earth (S11e).

  Finally, the filtrate was concentrated at 50 ° C. under reduced pressure to a Brix concentration of 75% to obtain an inositol fraction. Then, the inositol fraction was left as it was, and centrifuged (3000 rpm, 15 minutes) to precipitate 679 g of inositol crystals (S12).

  Table 1 below shows the purity of the obtained inositol crystals and the recovery rate of inositol recovered in S12 when the inositol contained in the diluted molasses before passing through the cation exchange column is 100%. .

[Example 2]
In this example, inositol was recovered by a method different from the inositol recovery method described in Example 1 above. A flowchart of the inositol recovery method of this example is shown in FIG.

  In Example 1 shown in FIG. 1, diluted molasses diluted to a Brix concentration of 28% was directly passed through a cation exchange column. In contrast, in this example, as shown in FIG. 2, diluted molasses diluted to a Brix concentration of 28% was allowed to stand at 5 ° C. for 3 days to precipitate crystals (S21), and centrifuged (3000 rpm, 15 The crystals were recovered after a minute (S22), and the recovered crystals were dissolved again in demineralized water to prepare diluted molasses (40L) diluted to a Brix concentration of 35% (S23).

  Next, an ion exchange process was performed using diluted molasses diluted to a Brix concentration of 35% (S24). Specifically, first, the solution was passed through a cation exchange column (Mitsubishi Kasei Co., Ltd., column size 25 cm × length 90 cm, SV2.0) filled with a strongly acidic cation exchange resin PK216 substituted with Na type. (S24a). And after passing, the diluted waste molasses remaining in the cation exchange column was pushed out with demineralized water, and the whole amount was collected (S24b).

  Next, the collected liquid was passed through an anion exchange column (Mitsubishi Kasei Co., Ltd., column size inner diameter 25 cm × length 110 cm, SV2.0) filled with weakly basic anion exchange resin WA30 ( S24c).

  After passing through the anion exchange column, the residual liquid of the anion exchange column is pushed out with demineralized water, and all of these liquids are recovered (S24d). The diatomaceous earth was filtered using (S24e).

  The filtrate was concentrated at 50 ° C. under reduced pressure to a Brix concentration of 75% to obtain an inositol fraction. Then, the inositol fraction was left as it was to precipitate 1,780 g of inositol crystals (S25).

  In this example, an operation for improving the inositol recovery rate was performed by recovering the inositol fraction that could not be recovered from the cation exchange column and the anion exchange column.

  Specifically, the inositol crystals obtained in S25 are concentrated to a Brix concentration of 65% using demineralized water, crystallized at 5 ° C., and then centrifuged (3000 rpm, 15 minutes) to obtain a solid fraction. The liquid which collect | recovered, melt | dissolved in demineralized water, and let it pass through a cation exchange column was prepared (S26). Next, this liquid is passed through a cation exchange column of the same kind as described above (S27a), the total amount of liquid passed is recovered (S27b), and the recovered liquid is passed through the same anion exchange column as above (S27c). The total amount of liquid flow was collected (S27d). Furthermore, diatomaceous earth filtration (S27e) was performed in the same manner as described above, and the filtrate was concentrated to a Brix concentration of 75% under reduced pressure at 50 ° C. to obtain an inositol fraction. Then, the inositol fraction was left as it was to precipitate a total amount of 1,890 g of inositol crystals (S28).

  Table 1 below shows the purity of inositol crystals in S25 and S28, and the recovery rate of inositol recovered in S28 when the inositol contained in the diluted molasses before passing through the cation exchange column is 100%. Show.

Example 3
In this example, inositol was recovered by a method different from the inositol recovery method described in Example 2 above. A flowchart of the inositol recovery method of this example is shown in FIG.

  In Example 2 shown in FIG. 2, dilute waste molasses diluted to a Brix concentration of 28% was crystallized to recover the crystals, and the crystals were dissolved again in demineralized water to be diluted to a Brix concentration of 35%. Waste molasses (40 L) was prepared. On the other hand, in the present Example, the crystal | crystallization collect | recovered by crystallization was melt | dissolved and the diluted waste molasses (39L) diluted and prepared to Brix concentration 20% was produced (S30-S33).

  Next, an ion exchange process was performed using diluted molasses (39L) diluted to a Brix concentration of 20% (S34). Specifically, first, it was passed through a cation exchange column (Mitsubishi Kasei Co., Ltd., column size inner diameter 25 cm × length 90 cm, SV2.0) filled with a strongly acidic cation exchange resin PK216 substituted with Na type. It was made to liquid (S34a). Then, after passing through, the diluted molasses remaining on the cation exchange column was pushed out with demineralized water, and the whole amount was collected (S34b).

  Next, the entire collected liquid was passed through an anion exchange column (Mitsubishi Kasei Co., Ltd., column size inner diameter 25 cm × length 110 cm, SV 2.0) filled with weakly basic anion exchange resin WA30. (S34c).

  After passing through the anion exchange column, the residual liquid of the anion exchange column is pushed out with demineralized water, and the whole amount is recovered (S34d), and the whole recovered solution is used with a standard supercell (Hyflo Supercell / Johnsmanville). And filtered through diatomaceous earth (S34e).

  Finally, the filtrate was concentrated at 50 ° C. under reduced pressure to a Brix concentration of 75% to obtain an inositol fraction. Then, the inositol fraction was left as it was to precipitate 1,013 g of inositol crystals (S35).

  Table 1 below shows the purity of the obtained inositol crystals and the recovery rate of inositol recovered in S35 when the inositol contained in the diluted molasses before passing through the cation exchange column is 100%. Show.

Example 4
Next, the present inventors recovered betaine using the ion exchange process of Example 1 described above. In FIG. 4, the flowchart of the betaine collection | recovery process of a present Example using the ion exchange process of Example 1 is shown.

  As shown in FIG. 1, the diluted waste molasses diluted to a Brix concentration of 28% was passed through a cation exchange column (S11a in FIG. 1), and the total amount of the passed liquid was recovered (S11b in FIG. 1). 0.5N sodium hydroxide was passed through the exchange column at SV2.0 to recover betaine adsorbed on the cation exchange resin (S40), and a betaine fraction was obtained (S41).

  Next, this betaine fraction was cooled to room temperature and centrifuged (3000 rpm, 15 minutes) (S42) to precipitate crude betaine crystals.

  Furthermore, in order to increase the purity of the crude crystals, the obtained crude crystals are dissolved in demineralized water to prepare diluted prepared molasses having a Brix concentration of 65% (S43). Was precipitated (S44).

  Table 2 below shows the crude crystals of betaine obtained and the purity of the crystals.

Example 5
In this example, betaine was recovered by a method different from the betaine recovery method described in Example 4 above. The flowchart of the betaine collection | recovery method of a present Example is shown in FIG.

  In Example 2 above, 0.5N sodium hydroxide was passed through the cation exchange column after passing through diluted waste molasses diluted to a Brix concentration of 28% at SV2.0. As shown in FIG. 4, 1.5N sodium hydroxide was passed through a cation exchange column at SV2.0 to recover betaine adsorbed on the cation exchange resin (S50) to obtain a betaine fraction. (S51).

  Next, this betaine fraction was cooled to room temperature and centrifuged (3000 rpm, 15 minutes) (S52) to precipitate betaine crude crystals.

  Furthermore, in order to increase the purity of the crude crystals, the obtained crude crystals were dissolved in demineralized water to prepare diluted prepared molasses having a Brix concentration of 65% (S53), and left as it was to precipitate betaine crystals ( S54).

  Table 2 below shows the crude crystals of betaine obtained and the purity of the crystals.

  The method according to the present invention makes it possible to easily recover inositol at low cost by utilizing a beet sugar production process. Moreover, if the method which concerns on this invention is used, betaine can also be collect | recovered easily at low cost.

  Therefore, not only can it contribute to the comprehensive use of sugar beet, but it can also be applied to the production of inositol-containing health foods and pharmaceuticals utilizing the physiological functions of inositol. It can also be applied to the production of betaine-containing health foods and pharmaceuticals utilizing the physiological function of betaine.

It is the flowchart of inositol collection | recovery which showed one Example of the method of this invention. It is the flowchart of inositol collection | recovery which showed the other Example of the method of this invention. It is the flowchart of inositol collection | recovery which showed the other Example of the method of this invention. 6 is a flow chart of betaine recovery showing another embodiment of the method of the present invention. 6 is a flow chart of betaine recovery showing another embodiment of the method of the present invention.

Claims (10)

A method for recovering inositol from molasses produced after collecting beet sugar from sugar beet,
The waste molasses is passed through an ion exchange column filled with an ion exchange resin to adsorb a substance different from inositol contained in the waste molasses, and the entire amount of liquid after passing through the ion exchange column is recovered. An ion exchange process,
And a crystallization step of concentrating the recovered liquid to precipitate inositol crystals.   2. The method according to claim 1, wherein in the ion exchange step, the waste molasses is passed through the ion exchange column, thereby adsorbing a substance that hinders inositol recovery to the ion exchange resin.   3. The method according to claim 1, wherein in the ion exchange step, the liquid collected by passing through the ion exchange column is filtered through diatomaceous earth.   In the ion exchange step, the molasses is passed through a cation exchange column filled with a cation exchange resin and an anion exchange column filled with an anion exchange resin. Item 4. The method according to any one of Items 1 to 3.   In the ion exchange step, the molasses is passed through a cation exchange column filled with a cation exchange resin, and then passed through an anion exchange column filled with an anion exchange resin. The method according to any one of claims 1 to 4.   5. The method according to claim 4, wherein in the ion exchange step, the liquid is repeatedly passed through the cation exchange column and the anion exchange column.   The method according to any one of claims 4 to 6, wherein betaine in molasses is adsorbed on the cation exchange resin, and the substance different from betaine is adsorbed on the anion exchange resin.   The cation exchange resin is a porous type, and is passed through a cation exchange column filled with a styrene-based strongly acidic cation exchange resin. The method described in 1.   9. The betaine adsorbed on the cation exchange resin is eluted by passing an aqueous solution of sodium hydroxide of a predetermined concentration through the cation exchange column after passing the waste molasses. The method according to any one of the above.   The method according to claim 9, wherein 0.5N to 1.5N of the aqueous sodium hydroxide solution is passed.

Images