JP2007022931A - Skin-protection cosmetic product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic product effective for promoting the restoration/regeneration of the barrier structure of the horny layer of the skin, capable of activating in vivo antioxidation enzyme in the skin and giving a transparent skin having a moisture retention function based on the normal skin barrier function of the natural skin. <P>SOLUTION: The cosmetic product contains calcium ion and/or magnesium ion and an SOD (superoxide dismutase) derived from a melon, especially SOD in a concentrated material of a melon of Vauclusien species. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a skin protective cosmetic containing calcium ions and / or magnesium ions and melon-derived SOD (superoxide dismutase), specifically, melon-derived SOD contained in a melon concentrate of Vauxurcian.

  Skin is directly or indirectly damaged by ultraviolet rays, smoking, air pollution, mental stress and irregular and nutritionally unbalanced eating habits, dry skin, atopic dermatitis, psoriasis, inflammation Have been forced to form wrinkles and spots. Use of various antioxidants (vitamin E, vitamin C derivatives, etc.), application of moisturizers (glycerin, trehalose, hyaluronic acid, collagen, etc.), intercellular lipids (ceramide, cholesterol) to prevent and ameliorate these symptoms Etc.), application of anti-inflammatory agents (allantoin, etc.), metabolic active substances (α-hydroxy acid, β-hydroxy acid, etc.) and physiologically active substances (vitamin A, β-glucan, linoleic acid, etc.) Has been planned. However, almost all of them have effective ingredients from the outside, and the fact that they have aimed to improve the skin by the effects of the ingredients themselves, there are few things that aim to improve the barrier function with the original ability of the skin itself It was. In addition, it is difficult to say that the effects of the components themselves have been sufficiently satisfactory in terms of transdermal absorbability and sustainability of each substance.

  The object of the present invention is to improve the reduction of skin barrier function by complementing the effect of calcium and / or magnesium ions on skin stratum corneum intercellular lipid production, and from melon that activates the skin's in vivo antioxidant enzymes Of SOD (superoxide dismutase), especially the melon-derived SOD activation effect contained in the Vauxurcian melon concentrate, thereby providing the skin with a normal normal moisturizing function The object is to provide a cosmetic that realizes a feeling of skin. In other words, the present invention aims to improve the skin by utilizing the inherent intrinsic function of the skin as a living body.

  The skin protective cosmetic according to the present invention is characterized by containing calcium ions and / or magnesium ions and melon-derived SOD (superoxide dismutase).

  Moreover, the above-mentioned skin protective cosmetic contains calcium ions, magnesium ions and melon-derived SOD.

  Further, the melon-derived SOD is characterized by being a melon-derived SOD contained in a Volksian melon concentrate.

  Moreover, the above-mentioned skin protective cosmetic is characterized by being a liposome dosage form.

  According to the present invention, skin keratin is obtained by containing calcium ions and / or magnesium ions and melon-derived SOD, particularly melon-derived SOD contained in a Volksian melon concentrate in a skin protective cosmetic. To provide cosmetics that can promote the repair and regeneration of the layer barrier structure and activate the in vivo antioxidant enzyme of the skin, thereby realizing a transparent skin having the normal normal moisturizing function. Can do.

  Hereinafter, embodiments according to the present invention will be described in detail.

  The skin protective cosmetic according to the present invention is derived from melon contained in calcium (Ca) ion and / or magnesium (Mg) ion and melon-derived SOD (superoxide dismutase), in particular, Vauxcleusian melon concentrate. It contains SOD, and can be prepared by appropriately blending components commonly used in skin protective cosmetics.

  Ca ions and / or Mg ions exert a complementary effect on intercellular adipogenesis in the skin stratum corneum. Intercellular lipids are the main components that fill cells between cells in the stratum corneum, and have a moisturizing function in the stratum corneum and a barrier function from external stimuli. In a state where intercellular lipids are reduced due to rough skin or the like, it is easy to receive external stimulation, and it is difficult to normally suppress moisture transpiration from the inside. Then, the fall of the skin moisturizing function and the barrier function can be recovered by complementing and adjusting the production of intercellular lipids with Ca ions and / or Mg ions.

Melon-derived SOD activates SOD, catalase, and glutathione peroxidase which are in vivo antioxidant enzymes, and is useful for decomposing superoxide and hydrogen peroxide H ₂ O ₂ which are active oxygen. In vivo antioxidant enzymes, SOD decomposes superoxide in the body into hydrogen peroxide H ₂ O ₂ and oxygen O ₂ , and catalase and glutathione peroxidase further decompose hydrogen peroxide H ₂ O ₂ produced by SOD This reduces the active oxygen. Excessive active oxygen oxidizes skin cells' proteins and lipids, reducing the function of cells and promoting aging. Therefore, supplying melon-derived SOD to the skin, activating the in vivo antioxidant enzyme and reducing excessive active oxygen, prevents skin aging and rough skin, and maintains the skin barrier structure in a healthy state・ You can recover.

  In the present invention, by combining Ca ions and / or Mg ions with melon-derived SOD, particularly melon-derived SOD contained in a melon concentrate of Vauxurcian species, intercellular lipid production of the stratum corneum of the skin is achieved. Not only the complementary effect and the activation effect of the in vivo antioxidant enzyme are exhibited independently, but both effects are exerted synergistically to further promote the skin moisturizing function, and the skin barrier function. The decrease can be recovered more effectively.

  The blending amount of Ca ions and / or Mg ions in the skin protective cosmetic is 0.0001 to 2.5 wt%, preferably 0.001 to 1.0 wt%, more preferably 0.01 to 0.5 wt%. is there. When the blending amount is less than 0.0001 wt%, the Ca ion and / or Mg ion complementing effect is difficult to be exhibited, and when the blending amount is more than 2.5 wt%, the stickiness on the skin feeling after application is likely to become strong, which is preferable. In addition, no further effects can be expected.

  Further, the weight ratio of Ca ions to Mg ions is 5: 1 to 1: 5, preferably 3: 1 to 1: 3, more preferably 2: 1 to 1: 2. Within this weight ratio range, it is possible to more effectively prevent the skin barrier function from being lowered. It should be noted that the skin barrier function can be supplemented by using only one of Ca ions and Mg ions.

  The blending amount of melon-derived SOD in the skin protective cosmetic is 0.0001 to 70.0 wt%, preferably 0.0001 to 3.0 wt%, more preferably 0.01 to 1.0 wt%. When the blending amount is less than 0.0001 wt%, the activation effect of the antioxidant enzyme in the living body is hardly exhibited, and when it is dispersed more than 70.0 wt% in the cosmetic, the stickiness tends to become strong, which is not preferred. I can't expect.

  The blending ratio of Ca ions and / or Mg ions and melon-derived SOD is 10: 1 to 1:10, preferably 5: 1 to 1: 5, more preferably 2: 1 to 1: 2 by weight. Is desirable. Within this weight ratio range, it is possible to more effectively prevent the skin barrier function from being lowered.

  The form of the skin protective cosmetic is not particularly limited, and examples thereof include cosmetics such as lotion, milky lotion, cream, packs, facial cleansers, massages, body cosmetics, and quasi drugs.

  As Ca ions, one or a combination of two or more of salts such as calcium chloride, calcium sulfate, calcium phosphate, and calcium lactate can be directly blended in the cosmetic. Similarly, as the Mg ions, one or a combination of two or more of salts such as magnesium chloride, magnesium sulfate, magnesium phosphate, and magnesium lactate can be directly blended in the cosmetic. One or more Ca ions and Mg ions can be blended from these salts to form a combination of Ca ions and Mg ions. Moreover, Ca ion and / or Mg ion can be mix | blended in cosmetics as refined deep sea water which contained Ca ion and Mg ion which processed deep sea water with the reverse osmosis membrane etc. suitably. Furthermore, it can mix | blend in cosmetics using the various mineral water and hard water (for example, hardness 100-10000) containing Ca ion and Mg ion suitably.

  The melon-derived SOD is not particularly limited as long as it is contained in various melons. In particular, melon-derived SOD contained in a Volksian melon concentrate is preferable. The Vauxulcian melon is a melon native to the Avignon region of France.

  In addition to the above-described components, skin protective cosmetics include, for example, oils, powders, lipids, surfactants, purified water, lower alcohols, polymer compounds, gelling, An agent, an ultraviolet absorber, an ultraviolet scattering agent, an antioxidant, a pigment, an antiseptic, a fragrance, a cosmetic ingredient, and the like can be appropriately selected and used as long as the effects of the present invention are not impaired.

  Lipids include natural or synthetic phospholipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, sphingomyelin, egg yolk lecithin, soybean lecithin, hydrogenated phospholipids, cholesterols, For example, cholesterol, alkyl ester of cholesterol, phytosterol, alkyl ester of phytosterol, glyceroglycolipid, acyl glucoside such as cetyl galactoside, dialkyl type synthetic surfactant, N-acyl-sphingosine or sulfate thereof, N-acylsphingo glycolipid , N-higher acyl glutathione, or a mixture of two or more thereof. In addition, in the liposome formed using a lipid or the like, a polyhydric alcohol, a higher alcohol, a higher fatty acid, or the like can be blended as necessary for stabilization or improvement of the phase transition temperature.

  As deep sea water, surface seawater is settled and forms a layer, it is low temperature, clean and natural water rich in nutrient salts, and the intake depth is usually 250 to 500 m, preferably 300, depending on the sea area. The thing of -400m or less can be mix | blended.

  As fats and oils, jojoba oil, castor oil, olive oil, soybean oil, coconut oil, palm oil, cacao oil, mink oil, turtle oil and the like can be blended.

  As the hydrocarbons, liquid paraffin, petrolatum, microcrystalline wax, squalane and the like can be blended.

  As waxes, beeswax, lanolin, carnauba wax, candelilla wax and the like can be blended.

  As the fatty acid, myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, lauric acid and the like can be blended.

  Synthetic esters include isopropyl myristate, isopropyl palmitate, butyl oleate, myristyl myristate, octyldodecyl myristate, propylene glycol monostearate, myristyl lactate, isostearyl malate, glyceryl monostearate, distearyl dimethyl chloride Ammonium etc. can be mix | blended.

  The above-described oils and fats, hydrocarbons, waxes, fatty acids, and synthetic esters are usually blended at a ratio of 0 to 30 w / w%.

  As alcohols, ethanol, 1,3-butylene glycol, propylene glycol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, and the like can be blended. Alcohols are usually blended at a rate of 0 to 25 w / w%.

  Surfactants include glycerin fatty acid esters, sorbitan fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene alkyl ethers, coconut oil fatty acid monoethanolamide, polyoxyethylene hydrogenated castor Oil, sodium lauryl sulfate, pyroglutamate isostearate polyoxyethylene glyceryl, sodium alkylbenzenesulfonate, polyoxyethylene stearyl ether, dialkylsulfosuccinate, cetylpyridinium bromide, -n-octadecyltrimethylammonium chloride, monoalkyl phosphate, N- Acyl sultamic acid, N-acyl glutamine, sucrose fatty acid ester, polyoxyethylene sorbitan monostearate, polyoxye Sodium Ren lauryl ether sulfate, and polyoxyethylene reduced lanolin can be formulated. The surfactant is usually added at a ratio of 0 to 10 w / w%.

  As the thickener, carboxyvinyl polymer, methylpolysiloxane, dextran, carboxymethylcellulose, carrageenan, hydroxypropylmethylcellulose and the like can be blended. A thickener is normally mix | blended in the ratio of 0-5 w / w%.

  As the humectant, glycerin, propylene glycol, 1,3-butylene glycol, pyroglutamic acid, acetylglutamic acid, hyaluronic acid, procyanidin and the like can be blended. A humectant is normally mix | blended in the ratio of 0-25 w / w%.

  As preservatives, benzoic acid, salicylic acid, dehydroacetic acid or salts thereof, phenols of paraoxybenzoic acid esters, triclosan halocarban, and the like can be blended. A preservative is normally mix | blended in the ratio of 0-0.3 w / w%.

  Any fragrance may be used as long as it is normally used in cosmetics.

  As the pigment, iron oxide, titanium dioxide, zinc oxide, kaolin, talc and the like can be blended. A pigment is normally mix | blended in the ratio of 0-5 w / w%.

  Drugs include wheat germ oil, vitamin A, vitamin B2, vitamin E, magnesium or sodium ascorbate-2-phosphate, D-pantotail alcohol, dipotassium glycyrrhizinate, glutathione, UV absorber, chelating agent, plant extract Products, microbial metabolites / extracts, and the like. The drug is usually blended at a rate of 0 to 5 w / w%.

  As water, tap water, mineral water, brine, seawater, ultrapure water, polar ice-derived water, mineral water, purified water, and the like can be blended. Water is blended in an arbitrary ratio.

  The dosage form of the cosmetic is arbitrary, and can be, for example, a solubilized emulsifier form or a dispersion form.

  Examples of the skin protective cosmetic according to the present invention include skin care products such as lotion, milky lotion, beauty essence, cream, face wash, pack, gel, and products such as emulsifying foundation.

  Examples according to the present invention will be described below. Note that the present invention is not limited to the embodiments.

Example 1
(Restoration ability of skin barrier function by combination of CaCl ₂ , MgCl ₂ and SOD derived from voorcian melon)
The test aqueous solution was applied to the skin of the subject, and the recovery ability of the skin barrier function was evaluated by calculating the barrier function recovery rate from the transdermal water loss (TEWL) value. The TEWL value indicates that the lower the value, the more moisture is retained in the skin and the higher the skin barrier function. The test was conducted on 11 healthy women, and these were averaged to determine the barrier function recovery rate.

The test aqueous solution contains CaCl ₂ as the Ca ion content, MgCl ₂ as the Mg ion content, and SOD derived from the Volksian melon as the melon-derived SOD in the blending amounts and blending ratios shown in Table 1, respectively. It was adjusted with water.

  The test was conducted in a sealed room controlled at a temperature of 20 ± 2 ° C. and a humidity of 35 ± 5 RH%, and the subject entered the room 30 minutes before measurement and was kept in a resting state.

After waiting, the stratum corneum on the inner side of the upper arm of the subject was peeled 15 times with scotch tape to reduce the skin barrier function. The TEWL value immediately after peeling was measured using TEWAMETER TM210 manufactured by Integral Co., Ltd. At this time, the TEWL value becomes 7.0 to 10.0 (mg / cm ² per hour) due to the peeling, but is usually 0.3 (mg / cm ² per hour) or less. After measuring the TEWL value by destroying the barrier function in this way, immediately apply 50 μl of the test aqueous solution, and then apply an ultraviolet lamp (photochemical / constant pressure mercury lamp 32W / UVL-32LB type) to generate active oxygen in the skin. Each test subject was irradiated with ultraviolet rays for 2 minutes in MED (minimal erythema dose) of each subject. Thereafter, the TEWL values after 3, 6 and 12 hours were followed and measured by the same method as described above. The barrier function recovery rate was determined from the TEWL value measured in this manner by Equation 1.

(Barrier function recovery rate) = [1-((TEWL value immediately after exfoliation treatment of stratum corneum) − (TEWL value after definite period of time)) / ((TEWL value immediately after exfoliation treatment of stratum corneum) − (Baseline of TEWL value) ))] X 100% (Formula 1)
Here, the baseline of the TEWL value is a value (so-called control) shown before the stratum corneum peeling treatment of each subject. The evaluation results are shown in Table 1.

  As shown in Table 1, sample No. 1 according to the present invention containing Ca ions and / or Mg ions and melon-derived SOD. 1 to 5 had a higher barrier function recovery rate than the other samples.

That is, sample no. Nos. 1 to 5 are sample numbers of melon-derived SOD only. 6 and no. Compared to 7, the barrier function recovery rate was high. And sample no. 1 to 3 are Nos. Of only CaCl ₂ / MgCl ₂ . Compared with samples having the same ratio of CaCl ₂ / MgCl ₂ to 8 to 10, the barrier function recovery rate was high at any elapsed time. In particular, when the ratio of CaCl ₂ / MgCl ₂ is 3/1 and 1/1, 2 and No. 9 and no. 3 and no. In 10, the difference was large.

Thus, it was found that by combining CaCl ₂ / MgCl ₂ and melon-derived SOD in combination, the ability to recover the skin barrier function can be further enhanced as compared with the case where each of them is added alone.

Sample No. Nos. 4 and 5 each contain only CaCl ₂ or MgCl ₂ and melon-derived SOD, but No. 4 containing only the same amount of melon-derived SOD. Compared to 7, the barrier function recovery rate was high.

Thus, it has been found that the ability to recover the skin barrier function can be further enhanced by combining either CaCl ₂ or MgCl ₂ and melon-derived SOD in combination.

(Example 2)
(Effectiveness test for skin barrier function by cream)
A cream was prepared according to the following formulation and the skin barrier function recovery rate was evaluated.

(Prescription Example 1)
(W / w%)
Deep sea water (reverse osmosis membrane filtered) 50.0
Jojoba oil 10.0
Phospholipid 0.75
Cholesterol 0.5
Glycerin fatty acid ester 2.0
Higher alcohol 3.0
Polyhydric alcohol fatty acid ester 0.5
CaCl ₂ / MgCl ₂ (1/1) 0.1
Melon-derived SOD 0.5
Xanthan gum 0.05
Glycerin 7.0
1,3-butylene glycol 7.0
Moisturizer 0.5
Preservative 0.5
Adjust the total amount of purified water to 100

  The manufacturing method of the cream followed the general method. The deep ocean water (reverse osmosis membrane filtered) used here is water obtained by treating deep ocean water with reverse osmosis membrane filtration. As a result of measuring the TEWL value by the same method as in Example 1 (however, the number of subjects: 2 healthy women and 4 men), the barrier function recovery rate was as high as 94% after 12 hours.

(Example 3)
(Effectiveness test for skin barrier function by liposome solution)
A liposome solution was prepared according to the following formulation, and the barrier function recovery rate was evaluated.

(Prescription example 2)
(W / W%)
Hydrogenated soybean lecithin 1.0
Cholesterol 0.5
Propylene glycol 3.0
Glycerin 3.0
CaCl ₂ / MgCl ₂ (1/1) 0.01
Melon-derived SOD 0.5
Xanthan gum 0.02
Trehalose 5.0
Preservative 0.5
Adjust the total amount of purified water to 100

In the production of the liposome solution, hydrogenated soybean lecithin, cholesterol, propylene glycol, glycerin, CaCl ₂ / MgCl ₂ (1/1), melon-derived SOD, and preservative are heated and dissolved at 80 ° C. and 30% at the same temperature. The aqueous trehalose solution was gradually added, and finally the remaining purified water was added, followed by cooling to room temperature. The liposome particle size was 175 nm in terms of weight average rate.

  As a result of measuring the TEWL value by the same method as in Example 1 (however, the number of subjects: 2 healthy women and 4 men), the barrier function effect recovery rate was as high as 92% even after 6 hours. After 12 hours, it was 96%. Thus, it can be seen that in the form of the liposome solution, the skin barrier function was almost restored after 6 hours.

Claims (4)

  A skin protective cosmetic comprising calcium ion and / or magnesium ion and melon-derived SOD (superoxide dismutase).   The skin protective cosmetic according to claim 1, comprising calcium ion, magnesium ion and SOD derived from melon.   The skin protective cosmetic according to claim 1 or 2, wherein the melon-derived SOD is a melon-derived SOD contained in a melon concentrate of Vauxulcian.   The skin protective cosmetic according to any one of claims 1 to 3, wherein the skin protective cosmetic is in a liposome dosage form.