JP2007014219A - Brewed vinegar and producing method of the same - Google Patents
Brewed vinegar and producing method of the same Download PDFInfo
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本発明は、飲用しやすく、血糖降下作用等の生理効果の優れた醸造酢及びその製造法に関する。 The present invention relates to a brewed vinegar that is easy to drink and has excellent physiological effects such as a hypoglycemic effect and a method for producing the same.
醸造酢には殺菌作用、食欲増進作用、減塩効果、ビタミンC破壊酵素抑制作用などの生理効果があることから、近年の健康ブームにより健康食品として注目されているが、血糖降下作用を有する醸造酢は知られていない。 Because brewed vinegar has physiological effects such as bactericidal action, appetite-promoting action, salt-reducing effect, and vitamin C-destructing enzyme-suppressing action, it has been attracting attention as a health food due to the recent health boom. Vinegar is not known.
一方、桑葉は、制糖作用、すなわち血糖降下作用及び糖吸収抑制作用を有することが知られており、1−デオキシノジリマイシン(以下、DNJと称する)が、桑葉の血糖降下作用を有する有効成分であることが知られている。DNJは、ブドウ糖(グルコース)に類似する構造からなり、ブドウ糖の拮抗物質としてα−グルコシダーゼの働きを阻害し、血糖の上昇を抑制するため、糖尿病予防・治療に有用であることが知られている(非特許文献1)。しかしながら、1−デオキシノジリマイシンは、桑葉中に極めて少量しか含有せず、抽出が困難であるという問題があった。
本発明の目的は、新たな生理機能を有する醸造酢及びその製造方法を提供することにある。 The objective of this invention is providing the brewing vinegar which has a new physiological function, and its manufacturing method.
本発明者らは、澱粉原料又は糖質原料を糖化及びアルコール発酵させ、次いで酢酸菌を加えて酢酸発酵する醸造酢の製造法において、糖化する際、アルコール発酵する際又は酢酸発酵する際に桑葉又はその粉砕物を加えることにより、飲用しやすくDNJを含み糖尿病等に有用な醸造酢が得られることを見出し、本発明を完成させた。 In the method for producing brewed vinegar in which starch raw material or saccharide raw material is saccharified and alcoholic fermented, and then acetic acid bacteria are added and acetic acid fermented, the mulberry is saccharified, alcoholic fermented or acetic acid fermented. It has been found that by adding leaves or a pulverized product thereof, a brewed vinegar that is easy to drink and contains DNJ and is useful for diabetes and the like has been obtained, and the present invention has been completed.
すなわち本発明は、澱粉原料又は糖質原料を糖化及びアルコール発酵させ、次いで酢酸菌を加えて酢酸発酵する醸造酢の製造法において、糖化する際、アルコール発酵する際又は酢酸発酵する際に桑葉又はその粉砕物を加えることを特徴とする醸造酢の製造法を提供するものである。 That is, the present invention relates to a method for producing brewed vinegar in which a starch raw material or a saccharide raw material is saccharified and subjected to alcohol fermentation, and then acetic acid is added and acetic acid fermented. In saccharification, alcohol fermentation or acetic acid fermentation, mulberry leaves Or the manufacturing method of the brewing vinegar characterized by adding the ground material is provided.
また本発明は、澱粉原料又は糖質原料を糖化及びアルコール発酵させ、次いで酢酸菌を加えて酢酸発酵する方法により得られる醸造酢であって、糖化する際、アルコール発酵する際又は酢酸発酵する際に桑葉又はその粉砕物を加える方法により得られる醸造酢を提供するものである。 The present invention is also a brewed vinegar obtained by saccharification and alcohol fermentation of a starch raw material or a saccharide raw material, followed by acetic acid fermentation by adding acetic acid bacteria, and when saccharifying, alcoholic fermentation or acetic acid fermentation It provides a brewed vinegar obtained by adding mulberry leaves or pulverized products thereof.
本発明の方法によれば、桑葉又はその粉砕物由来のDNJを多量に含み、血糖降下作用を有し、糖尿病、肥満等の治療改善に有用であって、かつ、風味が良好で飲みやすい醸造酢を得ることができる。 According to the method of the present invention, it contains a large amount of DNJ derived from mulberry leaves or a pulverized product thereof, has a hypoglycemic action, is useful for improving treatment of diabetes, obesity and the like, and has a good flavor and is easy to drink. You can get brewed vinegar.
本発明に用いられる澱粉質原料又は糖質原料としては、原料の種類に関係なく適用することができるが、具体的には、例えば、白砂糖、ザラメ、黒砂糖、三温糖等の砂糖類、米、麦、とうもろこし、はとむぎ等の穀類、さつまいも、ジャガイモ等の芋類、にんじん等の野菜類、りんご、ブドウ等の果実類を用いることができるが、本発明においては、砂糖類が好ましく、黒砂糖がより好ましい。これらは、単独で用いてもよく、また2種以上混合して用いてもよい。 The starch raw material or saccharide raw material used in the present invention can be applied regardless of the type of raw material, and specifically, for example, sugars such as white sugar, salame, brown sugar, and three-warm sugar. , Grains such as rice, wheat, corn, corn, sweet potatoes, potatoes, vegetables such as carrots, fruits such as apples and grapes, but in the present invention, sugars are preferred, Brown sugar is more preferred. These may be used singly or in combination of two or more.
本発明の方法において行われる澱粉原料又は糖質原料の糖化は、通常の方法で行えばよく特に制限されないが、澱粉原料又は糖質原料を蒸煮したものに、麹及び/又は酵素を加え反応させることにより行うことができる。反応は、例えば、18℃〜20℃で、1日〜5日の条件下で行うことができる。ここで用いられる麹としては、紅麹、黄麹、黒麹、白麹等が挙げられ、これらを単独で用いても組み合わせて用いてもよいが、紅麹及び黄麹を組み合わせて、又は黒麹及び黄麹を組み合わせて用いるのが好ましい。かように組み合わせて用いる場合、両者の質量比率は、4:6〜6:4であるのが好ましい。紅麹は、糖化力が弱く、DNJを安定に残存させるため特に好ましい。黒麹及び黄麹を組み合わせて用いれば、もろみの甘味を適切に調節できる点で好ましい。なお、糖化の際に、必要に応じて、大豆油をしぼり取った粉末やこんぶ等を加えてもよく、この場合、より風味がよくアミノ酸等の栄養が豊富な醸造酢を得ることができる。こんぶを加えることにより、グルタミン酸、食物繊維を含む醸造酢が得られるのみならず、食物を軟化し消化しやすく醸造酢が得られ、また、醸造酢特有の匂いや、刺激臭、不快な発酵臭が和らげられた醸造酢が得られる点で好ましい。 The saccharification of the starch raw material or saccharide raw material performed in the method of the present invention is not particularly limited as long as it is carried out by a usual method. However, the starch raw material or the saccharide raw material is steamed and reacted with koji and / or enzyme. Can be done. The reaction can be carried out, for example, at 18 ° C. to 20 ° C. under conditions of 1 day to 5 days. Examples of the cocoons used here include red potatoes, yellow cocoons, black cocoons, and white cocoons. These may be used alone or in combination. It is preferable to use a combination of straw and jaundice. When used in such a combination, the mass ratio of the two is preferably 4: 6 to 6: 4. Red yeast rice is particularly preferred because it has a weak saccharification ability and allows DNJ to remain stably. Use of a combination of black candy and yellow candy is preferred in that the sweetness of moromi can be adjusted appropriately. In addition, during saccharification, if necessary, powder or kumbu squeezed soybean oil may be added, and in this case, brewed vinegar that is more flavorful and rich in nutrients such as amino acids can be obtained. By adding kumbu, not only brewed vinegar containing glutamic acid and dietary fiber can be obtained, but also brewed vinegar can be obtained by softening and easily digesting food. Is preferable in terms of obtaining a brewed vinegar that has been tempered.
本発明の方法において行われるアルコール発酵は、かくして糖化された澱粉原料又は糖質原料に、酵母を加え発酵させることにより行うことができる。発酵は、通常の方法で行えばよく特に制限されないが、例えば、18℃〜20℃で、1日〜5日の条件下で行うのが好ましい。18℃未満であるとアルコール発酵が抑制され、20℃を超えるとアルコール発酵が急速すぎる点で好ましくない。なお、糖化およびアルコール発酵は、同一の系内で行うのが好ましく、麹を加えて発酵液面に麹を浮かせて振り麹とした後に、直ちに酵母を加え、その後18℃〜20℃で2日〜10日間糖化の反応およびアルコール発酵を行うのが好ましい。得られた発酵液は、アルコール濃度が10%〜20%であるのが好ましい。なお、アルコール発酵液中のアルコール濃度を調整するため、アルコールを新たに添加してもよい。 The alcohol fermentation performed in the method of the present invention can be performed by adding yeast to the starch raw material or saccharide raw material thus saccharified and fermenting. Fermentation may be performed by a normal method and is not particularly limited. For example, the fermentation is preferably performed at 18 ° C. to 20 ° C. for 1 to 5 days. Alcohol fermentation is suppressed as it is less than 18 degreeC, and when 20 degreeC is exceeded, it is not preferable at the point which alcohol fermentation is too rapid. Note that saccharification and alcohol fermentation are preferably performed in the same system. After adding koji and floating koji on the surface of the fermentation broth, adding yeast immediately, and then at 18 ° C to 20 ° C for 2 days. The saccharification reaction and alcohol fermentation are preferably performed for 10 days. The obtained fermentation broth preferably has an alcohol concentration of 10% to 20%. In addition, in order to adjust the alcohol concentration in the alcohol fermentation liquid, alcohol may be newly added.
本発明で行われる酢酸発酵は、かくして糖化及びアルコール発酵されたものに酢酸菌を加えて発酵させることにより行われる。発酵は、通常の方法で行えばよく特に制限されないが、酢酸発酵の条件は、気温の変化などに応じて適宜選択するのが好ましく、例えば、酢酸菌を加えたのち、18℃〜20℃で、90日〜120日の条件下で行うのが好ましい。また、本発明においては、酢酸発酵の際に、酢酸菌を加えてから40日目〜50日目に1〜3回空気を取り入れて攪拌を行うことが好ましい。ここで用いられる酢酸菌としては、エタノールを酸化して酢酸を生成することができる微生物、すなわちエタノール脱水素酵素及びアルデヒド脱水素酵素を産生する微生物であれば遺伝子組換え微生物も含め特に制限されるものではなく、アセトバクター・アセチ(Acetobacter aceti)等の公知の酢酸菌を好適に例示することができる。加える酢酸菌の量は、発酵液に対して乾燥物質量換算で0.0001質量%〜0.001質量%であるのが好ましい。 The acetic acid fermentation performed in the present invention is performed by adding an acetic acid bacterium to the saccharified and alcohol-fermented product and fermenting it. Fermentation is not particularly limited as long as it is performed in a normal manner, but the conditions for acetic acid fermentation are preferably appropriately selected according to changes in temperature, for example, at 18 to 20 ° C. after adding acetic acid bacteria. , It is preferably performed under the condition of 90 days to 120 days. Moreover, in this invention, it is preferable to take in 1 to 3 times and stir in the 40th-50th day after adding an acetic acid bacteria in the case of acetic acid fermentation. The acetic acid bacteria used here are particularly limited as long as they are microorganisms that can oxidize ethanol to produce acetic acid, that is, microorganisms that produce ethanol dehydrogenase and aldehyde dehydrogenase. It is not a thing but well-known acetic acid bacteria, such as Acetobacter aceti (Acetobacter aceti), can be illustrated suitably. The amount of acetic acid bacteria to be added is preferably 0.0001% by mass to 0.001% by mass in terms of dry matter relative to the fermentation broth.
本発明の醸造酢の製造法においては、桑葉又はその粉砕物を加えることが必要である。加える時期としては、糖化する際、アルコール発酵する際又は酢酸発酵する際であればよく、発酵状況をみながら適宜選択するのが好ましいが、例えば、澱粉原料又は糖質原料を蒸煮したものに麹及び/又は酵素を加える前、糖化の反応開始後1日〜4日、酵母を加えると同時、アルコール発酵開始後1日〜4日、酢酸菌を加えると同時、酢酸発酵開始後5日〜20日、特に8日〜12日などが挙げられ、このうち、麹及び/又は酵素を加える前、又は酢酸発酵開始後8日〜12日が好ましい。加えられる桑葉又はその粉砕物は、乾燥物質量換算で、反応液中6〜10質量%の範囲で用いるのが、抽出の効率及び風味の観点から好ましい。粉砕物の粒径としては、10μm〜10cm、特に5cm〜8cmであるのが好ましい。 In the manufacturing method of the brewing vinegar of this invention, it is necessary to add a mulberry leaf or its ground material. The timing of addition may be any time during saccharification, alcohol fermentation or acetic acid fermentation, and is preferably selected as appropriate while observing the fermentation status. And / or before adding the enzyme, 1 to 4 days after the start of the saccharification reaction, simultaneously with the addition of yeast, 1 to 4 days after the start of alcoholic fermentation, simultaneously with the addition of acetic acid bacteria, and 5 to 20 days after the start of acetic acid fermentation Day, especially 8-12 days, etc. are mentioned, and among these, before adding straw and / or enzyme, or 8-12 days after the start of acetic acid fermentation is preferable. It is preferable from the viewpoint of extraction efficiency and flavor that the mulberry leaves to be added or the pulverized product thereof are used in the range of 6 to 10% by mass in terms of dry substance. The particle size of the pulverized product is preferably 10 μm to 10 cm, particularly preferably 5 cm to 8 cm.
かくして得られる発酵液を、必要に応じてろ過、殺菌することにより本発明の醸造酢が得られる。得られる醸造酢には、さらに例えば6ヶ月〜1年間熟成させれば、より深い味わいが得られる点で好ましい。かくして得られる醸造酢は、DNJを含み、血糖降下作用を有し、糖尿病、肥満等の治療改善に有用であって、かつ、風味に優れ飲みやすい。かかる醸造酢は、調味料としてそのまま使用することもできるが、他の副素材と混合して、加工酢、ドレッシング類、ソース類、スープ類、惣菜、飲料等の各種飲食品へ利用することもできる。加工酢としては、ポン酢、すし酢、サラダ酢、ラッキョウ酢及びその他の野菜酢漬類、海産酢漬類等が挙げられる。 The brewed vinegar of the present invention is obtained by filtering and sterilizing the fermentation broth thus obtained as necessary. The brewed vinegar obtained is preferable in that it can be further aged for, for example, 6 months to 1 year in order to obtain a deeper taste. The brewed vinegar thus obtained contains DNJ, has a hypoglycemic action, is useful for improving treatment of diabetes, obesity and the like, and has a good flavor and is easy to drink. Such brewed vinegar can be used as a seasoning as it is, but it can also be used as a seasoning for various foods and beverages such as processed vinegar, dressings, sauces, soups, side dishes, and beverages. it can. Examples of processed vinegar include ponzu, sushi vinegar, salad vinegar, rakkyo vinegar and other vegetable vinegars, marine vinegars, and the like.
以下に実施例を示し、本発明をさらに詳しく説明する。しかしながら、本発明はこれら実施例に限定されるものではない。 The following examples illustrate the present invention in more detail. However, the present invention is not limited to these examples.
実施例1
容量70Lの陶製かめ壷に、澱粉原料として1Kgの黒砂糖を含む50Lの黒砂糖液、紅麹(ヤエガキ醗酵技研株式会社製)1Kg、黄麹1Kg、及び粉砕した桑葉(粒径10μm〜5mm)4kgを入れ、酵母20mLを接種し、麹を液面に浮かせて振り麹として、5日間発酵させた。その後、もろみ状態を確認した上で、純粋培養した公知の酢酸菌20mLと種酢15mLがよくなじむように混ぜたものを投入した。酢酸菌添加から100日目に醸造液をろ過して醸造酢を得た。なお、酢酸菌添加から40日目に空気を取り入れ空気攪拌を行った。得られた醸造酢の酸度は6%であり、DNJ濃度は12.5mg/100gであった。得られた醸造酢は、その酸味は通常の市販の米酢と同等のものであり、また、風味がよく飲用しやすいものであった。
Example 1
A 70-liter pottery pot, 50-liter brown sugar solution containing 1 kilogram of brown sugar as a raw material for starch, 1 kilogram of red rice cake (manufactured by Yaegaki Fermentation Co., Ltd.), 1 kilogram of yellow koji, and ground mulberry leaves (particle size: 10 μm to 5 mm) ) 4 kg was added, 20 mL of yeast was inoculated, and the koji was floated on the liquid surface and fermented for 5 days. Then, after confirming the moromi state, a well-mixed well-known acetic acid bacterium (20 mL) and seed vinegar (15 mL) were added. On the 100th day after the addition of acetic acid bacteria, the brewing liquid was filtered to obtain brewed vinegar. In addition, air was introduced and stirred on the 40th day after the addition of acetic acid bacteria. The obtained brewed vinegar had an acidity of 6% and a DNJ concentration of 12.5 mg / 100 g. The obtained brewed vinegar had an acidity equivalent to that of ordinary commercially available rice vinegar, and had a good flavor and was easy to drink.
実施例2
容量70Lの陶製かめ壷にブリックス12%〜15%の黒砂糖水溶液50Lを用意し、ソルピーM60〔大豆油をしぼり取った粉末:日清オイリオ株式会社製〕1Kg、塩分抜きこんぶ0.5gを50L糖水溶液と混合して投入し、黒麹1Kg及び黄麹1Kgを液面に浮かせ振り麹とし、さらに直ちに酵母水溶液100mLを接種し、その後5日間発酵させたのち、純粋培養した種酢100mLを加え更に発酵させた。10日後酢酸菌膜が壷口の半分くらいに拡張していたものに、その菌膜を破損しないように注意しながら、桑葉乾燥ちぎり葉〔桑葉乾燥物を機械で裁断5cm〜8cm程度〕3Kgを投入した。その後発酵及び熟成を続け、仕込みから105日目に醸造酢をろ過して30Lの醸造酢を得た。得られた醸造酢は、その酸味は通常の市販の米酢と同等のものであり、また、風味が実施例1のものよりもよく、飲用しやすいものであった。
Example 2
Prepare 50L of Brix 12% to 15% brown sugar solution in a 70L pottery porridge, 1kg of Solpy M 60 [powdered soybean oil: made by Nisshin Oilio Co., Ltd.], 50L of salted kumbu 0.5g Mix with sugar aqueous solution, add 1Kg of black rice cake and 1Kg of yellow rice cake to the liquid surface to make a shaker. Immediately inoculate with 100mL of yeast aqueous solution, then ferment for 5 days, and then add 100mL of purely cultivated seed vinegar. Further fermented. 10 days later, the dried mulberry leaves were cut to about 5cm to 8cm by machine, taking care not to damage the fungal membranes after the acetic acid bacteria film had expanded to about half of the mouth. 3 kg was added. Fermentation and aging were then continued, and the brewed vinegar was filtered on the 105th day after preparation to obtain 30 L of brewed vinegar. The obtained brewed vinegar had an acidity equivalent to that of ordinary commercially available rice vinegar, and the flavor was better than that of Example 1 and was easy to drink.
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JP2019041994A (en) * | 2017-08-31 | 2019-03-22 | アース製薬株式会社 | Method for inhibiting acetic acid pungent odor |
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