JP2006517090A - 合成遺伝子 - Google Patents

合成遺伝子 Download PDF

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Publication number
JP2006517090A
JP2006517090A JP2004540296A JP2004540296A JP2006517090A JP 2006517090 A JP2006517090 A JP 2006517090A JP 2004540296 A JP2004540296 A JP 2004540296A JP 2004540296 A JP2004540296 A JP 2004540296A JP 2006517090 A JP2006517090 A JP 2006517090A
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vector
sequence
synthon
site
gene
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JP2006517090A5 (enExample
Inventor
ダニエル ブイ. サンティ,
ラルフ シー. リード,
サラ ジェイ. コドゥマル,
セバスチャン ジャヤラジ,
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コーサン バイオサイエンシーズ, インコーポレイテッド
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Publication of JP2006517090A publication Critical patent/JP2006517090A/ja
Publication of JP2006517090A5 publication Critical patent/JP2006517090A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2004540296A 2002-09-26 2003-09-26 合成遺伝子 Pending JP2006517090A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41408502P 2002-09-26 2002-09-26
PCT/US2003/030940 WO2004029220A2 (en) 2002-09-26 2003-09-26 Synthetic genes

Publications (2)

Publication Number Publication Date
JP2006517090A true JP2006517090A (ja) 2006-07-20
JP2006517090A5 JP2006517090A5 (enExample) 2006-12-07

Family

ID=32043342

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2004540296A Pending JP2006517090A (ja) 2002-09-26 2003-09-26 合成遺伝子

Country Status (5)

Country Link
US (3) US20040166567A1 (enExample)
EP (1) EP1576140A4 (enExample)
JP (1) JP2006517090A (enExample)
AU (1) AU2003277149A1 (enExample)
WO (1) WO2004029220A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Spiber株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
JP5089984B2 (ja) * 2003-10-03 2012-12-05 プロメガ コーポレイション 方向性クローニング用ベクター
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP1733055A4 (en) * 2004-02-27 2009-03-11 Harvard College polynucleotide
US20050227316A1 (en) * 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
AU2005295351A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
WO2006069099A2 (en) * 2004-12-21 2006-06-29 Genecopoeia, Inc. Method and compositions for rapidly modifying clones
JP2008526259A (ja) * 2005-01-13 2008-07-24 コドン デバイシズ インコーポレイテッド 蛋白質デザインのための組成物及び方法
WO2006081177A2 (en) * 2005-01-24 2006-08-03 Decode Biostructures, Inc. Gene synthesis software
ATE545701T1 (de) * 2005-05-17 2012-03-15 Ozgene Pty Ltd Sequentielles klonierungssystem
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof
US20070004041A1 (en) * 2005-06-30 2007-01-04 Codon Devices, Inc. Heirarchical assembly methods for genome engineering
US20070184487A1 (en) * 2005-07-12 2007-08-09 Baynes Brian M Compositions and methods for design of non-immunogenic proteins
WO2007009082A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
US20090087840A1 (en) * 2006-05-19 2009-04-02 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US20100291633A1 (en) * 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
DK2294407T3 (en) * 2008-06-06 2017-05-15 Dna Twopointo Inc SYSTEMS AND PROCEDURES FOR DETERMINING PROPERTIES THAT AFFECT THE EXPRESSION PROPERTY VALUE OF POLYNUCLEOTIDES IN AN EXPRESSION SYSTEM
US8551545B2 (en) * 2008-11-18 2013-10-08 Kraft Foods Group Brands Llc Food package for segregating ingredients of a multi-component food product
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2395087A1 (en) * 2010-06-11 2011-12-14 Icon Genetics GmbH System and method of modular cloning
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン9・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
US10331146B2 (en) 2013-03-15 2019-06-25 Lantheus Medical Imaging, Inc. Control system for radiopharmaceuticals
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
EP3688167A4 (en) * 2017-09-29 2021-07-14 Victoria Link Limited SYSTEM FOR MODULAR DNA ARRANGEMENT
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
JPH07313166A (ja) * 1993-01-25 1995-12-05 American Cyanamid Co 昆虫毒素AaITに関するコドン最適化DNA配列
JPH11511334A (ja) * 1995-09-22 1999-10-05 ザ ジェネラル ホスピタル コーポレーション 蛋白質の高レベル発現
WO2000063361A2 (en) * 1999-04-16 2000-10-26 Kosan Biosciences, Inc. A multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
WO2001092991A2 (en) * 2000-05-30 2001-12-06 Kosan Biosciences, Inc. Design of polyketide synthase genes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824513A (en) * 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
US6066721A (en) * 1995-07-06 2000-05-23 Stanford University Method to produce novel polyketides
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US6358712B1 (en) * 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US7001748B2 (en) * 1999-02-09 2006-02-21 The Board Of Trustees Of The Leland Stanford Junior University Methods of making polyketides using hybrid polyketide synthases
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
EP1227157A1 (en) * 2001-01-19 2002-07-31 Galapagos Genomics B.V. Swap/counter selection: a rapid cloning method
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
JPH07313166A (ja) * 1993-01-25 1995-12-05 American Cyanamid Co 昆虫毒素AaITに関するコドン最適化DNA配列
JPH11511334A (ja) * 1995-09-22 1999-10-05 ザ ジェネラル ホスピタル コーポレーション 蛋白質の高レベル発現
WO2000063361A2 (en) * 1999-04-16 2000-10-26 Kosan Biosciences, Inc. A multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
WO2001092991A2 (en) * 2000-05-30 2001-12-06 Kosan Biosciences, Inc. Design of polyketide synthase genes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
JPN6009057907, Nucleic Acids Res.,Vol.16,No.22(1988)p.10453−10467 *
JPN6009057908, Nucleic Acids Res.,Vol.16,No.5(1988)p.1693−1702 *
JPN6009057909, Biotechniques,Vol.16,No.6(1994)p.1116−1123 *
JPN6009057910, Nucleic Acids Res.,Vol.30,No.10(2002.May)p.e43 *
JPN6009057911, Science,Vol.282,No.5386(1998)p.63−68 *
JPN6009057912, Proc.Natl.Acad.Sci.USA.,Vol.96,No.5(1999)p.1846−1851 *
JPN6009057913, Protein Sci.,Vol.6,No.8(1997)p.1653−1660 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Spiber株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法

Also Published As

Publication number Publication date
EP1576140A2 (en) 2005-09-21
WO2004029220A2 (en) 2004-04-08
WO2004029220A3 (en) 2006-04-06
AU2003277149A1 (en) 2004-04-19
US20080274510A1 (en) 2008-11-06
US20080261300A1 (en) 2008-10-23
EP1576140A4 (en) 2007-08-08
US20040166567A1 (en) 2004-08-26
AU2003277149A8 (en) 2004-04-19

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