JP2006325420A - Experimental small animal-breeding container - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a breeding container in which an experimental small animal connected with a tube for administering a medicine or recovering body fluid, can move freely and also is not entangled with the tube or bits off the same. <P>SOLUTION: This container is constituted by a container body (11) and a freely attachable and detachable lid (12) is provided by connecting a canula tube (22) for recovering the body fluid to a mouse (21) bred in a cage, laying rails (12a), (12b) on which a carriage (13) can move, on the lid (12) of the cage, fixing a freely moving joint (23) on the carriage, joining the part of the canula tube with them and the other end to a container (25) for recovering the body fluid and breeding the mouse under a comfortable environment while supplying nutrition from feeds or a container (31) containing water. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a small animal breeding container for experiments for breeding small laboratory mammals, for example, mice for medical research, etc. in a comfortable environment.

  In the development of pharmaceuticals, confirmation of therapeutic effects by rats and mice and confirmation of the influence on the living body are routinely performed. These small animals may be kept connected with tubes (hereinafter referred to as cannula tubes) to administer drugs and collect body fluids, but this is not comfortable for rats and mice, Proper pharmacological effects cannot be obtained, or another metabolism occurs due to stress.

  Small laboratory animals are generally kept in transparent resin containers (cages), but in animal experiments using rats, mice, etc., with a cannula tube connected to a catheter attached to the neck of these animals. In the case of breeding, there is a problem that a cannula tube gets entangled every time a rat or mouse moves around, and this becomes stress and bites the tube. As one countermeasure, a breeding container having a free moving device as shown in FIG. 7 is used.

  In this apparatus, a mounting base (52) is attached to a transparent resin container (51), and a balance adjusting instrument (53) and a balance arm (54) are set on the base. Next, the free moving wire (55) is fixed to the cannula sieve (56), and the cannula sieve is fixed to the balance arm. At this time, the inclination of the balance arm is adjusted. On the other hand, the collar is fixed so as not to be detached from the rat neck. Connect and connect the connector tube to the bypass introduced from the back of the neck of the rat, cut it into a connector tube that connects to the cannula sieve and cut it to a length that will not loosen.

  By using this device, the balance arm and free moving wire respond quickly to the animal's quick movements, so it can be experimented under normal physiological conditions for a long time. When applied to mice with a small number of mice, there is a problem that it is difficult to adjust the balance and it is extremely difficult to rear normally.

In addition, the blood collection tube attached to the rat is wound around the pulley so that the rat does not get entangled or bite, and it does not loosen even if the rat moves around the breeding container with the weight tension attached to one end Although a blood sampling device has been announced, it is also difficult to adjust the balance with the weight, and it is not suitable for breeding a light animal such as a mouse. (For example, as in Patent Document 1.)
Japanese Patent Laid-Open No. 2004-041389

  Another reason why the mouse is not suitable for a light weight mouse is that the cannula tube itself is hard and lacks flexibility, so the mouse cannot move freely. In addition, the mouse is flipped off by the movement. Furthermore, the resistance when following the movement of the mouse is large, so that the mouse feels stress and cannot obtain appropriate data. In addition, the arm may be removed from the cage together with the mouse for cleaning the inside of the container, but it is difficult when the moving mouse is connected. Also, when handling a mouse, the collar may come off.

  The present invention has been made paying attention to the problems of the prior art as described above, and the gist of the present invention is that the small experimental animals can move freely and lightly without being restrained in the breeding container, and the cannula tube is entangled. It is an object of the present invention to provide a small animal breeding container for experiments that can be removed, cut, and not damaged, and that can perform breeding and biological experiments efficiently.

  The present invention is a small animal breeding container for experiment composed of a container body and a detachable lid, the carriage is provided with a carriage that operates following the movement of the U-shaped groove and the small animal for experiment, A moving joint is fixed to the cart, and a small animal breeding container for experiment, in which a tube having one end attached to the small experiment animal is connected to the joint.

  In the small animal breeding container for experiment, a rail is provided in which the carriage operates in the longitudinal direction of the lid, and both ends of the rail are provided with car stoppers, and a joint fixed to the carriage of the lid is freely movable and connected. A swivel joint with a rotating tube is desirable. The tube connected to the joint and the small experimental animal can be easily detached, and the tube connected to the small experimental animal is preferably composed of a double structure of a flexible polyurethane tube and a protective coil. .

The breeding container according to the present invention is provided with a cart that moves lightly in the lid portion, and a cannula tube is attached to the cart so that the mouse can move freely.
In addition, since the swinging mechanism is provided in the part where the cannular tube is attached to the carriage, it can cope with the intense movement of the mouse and the tube does not get tangled.
In addition, the cannula tube is made of a soft material that is extremely thin and has a double structure with a coil spring.

In addition, a U-shaped groove is provided in the lid of the breeding container so that the mouse can be easily removed while the cannula tube is connected, so that surgery and daily care are greatly improved.
In addition, this makes it possible to avoid stress disorder by comfortably moving the laboratory small animals that are raised.

Hereinafter, experimental small animal breeding containers (hereinafter simply referred to as breeding containers) according to embodiments of the present invention will be described with reference to FIGS. 1 to 5, but the present invention is not limited thereto.
The breeding container of the present embodiment is composed of a container body (hereinafter referred to as a cage) and a detachable lid, and the lid is adapted to move a U-shaped groove and a small experimental animal (hereinafter referred to as a mouse). A cart that follows and operates is provided. Further, a joint that is freely rotated or moved following the movement of the mouse is attached to the carriage, and a tube having one end connected to the mouse is attached to this joint.

  Hereinafter, embodiments will be described in detail. In addition, the same code | symbol is used for a common part. In FIG. 1, (11) is a cage made of transparent resin, (12) is a lid of the cage, and a lightly moving carriage (13) is attached to this lid. Reference numeral (31) shown in FIG. 1 is a container for supplying food or water to a mouse (21) bred in a cage (11).

  As shown in FIG. 2, the cage lid (12) has a structure in which stainless steel round bars are joined to each other at an arbitrary interval, and rails (12a) and (12b) are joined to the upper part thereof to form a carriage (13 Wheel (13a) moves on the rails (12a) and (12b). A freely movable joint (23) is fixed to the carriage (13), and a cannula tube (22) and a silicon tube (24) are connected to both ends thereof.

  The joint (23) is suspended so as to follow the direction in which the carriage (13) moves. The cannula tube (22) connected to one end of the joint (23) It has a structure that can rotate freely at the part. The secretions and the like of the mouse (21) bred in the cage (11) are collected from the cannula tube (22) through the joint (23) through the silicon tube (24) into the collection bottle (25).

FIG. 3 is a sectional view of the structure of the carriage (13) and the fixed joint (23). (A) is a view of the movement direction of the carriage (13) from the front, and (B) is It is the figure seen from the side.
Further, in FIG. 3, (13a) is a bar for suspending the joint (23).
The suspended joint (23) is connected to a cannula tube (22) and a silicon tube (24), respectively, but the cannula tube (22) to which the mouse (21) is connected is a carriage (13 ) Moves like a pendulum in the movement direction.

  FIG. 3B viewed from the side of FIG. 3 shows this state. Tubes are connected to the upper and lower ends of the joint (23), but the inside of the joint has a structure with a through hole (23a) so that secretions excreted from the mouse (21) can easily flow. Liquid leakage is prevented by a special seal (not shown).

4A and 4B are perspective views showing the structure of a cage lid in which stainless steel round bars are joined to each other at an arbitrary interval. FIG. 4A is a view showing the entire structure, and FIG. It is an enlarged view shown.
A mouse (not shown) bred in a cage is connected to a cannula tube (22), but since the cage lid is provided with a U-shaped groove, the cannula tube (22 ) Can be moved as indicated by an arrow in the enlarged view (B), and the mouse connected to the cannula tube (22) can be easily taken out from the cage.

FIG. 5 is a cross-sectional view showing the internal structure of a part of the cannular tube.
The cannular tube (22) has a double structure of a flexible polyurethane tube (22b) and a compression coil spring (22a). When recovering secretions from a rearing mouse, it is pushed out of the body by the secretion pressure. For this purpose, an extremely thin tube having a low softness and volume is preferable. The coil is preferably light, flexible and strong enough not to be caught by the mouse.

Hereinafter, as a specific example using the breeding container according to the present invention, an example in a bile excretion test of SCID mice will be given and described below.
A stainless steel round bar with a diameter of 2mm, and a carriage (about 40mm) with 4 plastic lightly moving wheels (13b) on the top lid of the cage (about 270mm x 150mm) (12) welded and joined at about 10mm intervals A lid (12) was prepared by suspending a joint (23) of about 10 g, which was mounted with (× 60 mm) (13) and moved more freely.

  On the other hand, a thin tube for collecting bile is inserted into the gallbladder of a SCID mouse (body weight 20.88 g) (21), a cannula tube (tube inner diameter is 1.2 mm) (22) is connected to this, and one end of the tube is connected to the present invention. Connected to the joint (23) provided on the container carriage (13).

  Place the SCID mouse (21) connected to the cannula tube (22) in a transparent plastic cage (about 260mm x 160mm x 140mm) (11), cover the top (12), and the other side of the joint is a silicone tube ( 24) was connected, and the end of the silicon tube (24) was connected to a bile collection container (25).

In this state, the amount of bile discharged from the SCID mouse (21) was measured every hour. The integration of collecting bile continuously for 53 hours is shown by the curve (A) in FIG.
While the SCID mouse (21) was bred in the container of the present invention, the carriage connected to the cannula tube (22) following the movement of the SCID mouse (21) moved (13) lightly and smoothly, In addition, it was confirmed that the cannula tube (22) moved around without being entangled with the mouse (21).

Comparative example

FIG. 6B shows an example in which bile was collected from the SCID mouse (21) in a restrained state where the limbs were fixed to the table with a tape.
Although it could be collected without problems until 4 hours, some blood was mixed in at 5 hours, and bile was hardly recovered after that.

It is the schematic of the experimental small animal breeding container which shows one Example of this invention. It is a partially broken perspective view showing an embodiment of a cage lid according to the present invention. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows an embodiment of the present invention, in which (A) is a sectional view of a rail and a carriage provided on a cage lid, and (B) is a cross-sectional view of a joint fixed to the carriage. It is a perspective view of the cage lid by this invention, (A) is a general view, (B) is a partially expanded view. It is a partial cutaway sectional view showing an example of a cannula tube according to the present invention. It is a graph which shows the Example result of a bile excretion test using the SCID mouse by this invention, and a comparative example result. It is explanatory drawing of the breeding container provided with the free moving apparatus of the prior art example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Cage 12 Cage lid | cover 12a, 12b Carriage moving rail 13 Car 13a Joint suspension rod 13b Wheel 14 Carriage stop 21 Small animal for experiment 22 Cannula tube 22a Compression spring coil 22b Tube 23 Joint 23a Joint connection 24 Silicon tube 25 Secretion collection container 31 Bait container

Claims (3)

  In a laboratory small animal breeding container composed of a container body and a detachable lid, a U-shaped groove and a carriage that follows the movement of the laboratory small animal are provided in the lid, and the carriage A small animal breeding container for experiment, wherein a freely movable joint is fixed, and a tube having one end attached to the small experiment animal is connected to the joint.   The small animal breeding container for experiment according to claim 1, wherein a rail for moving the carriage in the longitudinal direction is provided on the lid portion, and both ends of the rail are provided with a car stopper.   The experimental small animal breeding container according to the first aspect, wherein the joint fixed to the carriage is a swivel joint that freely moves, and one end of a tube connected to the small experimental animal can be easily connected.

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