JP2006316006A - Chitosan compounded composition and food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problems of chitosan having physiological activities such as cholesterol controlling action at the risk of the constipation side action to cause the difficulty in continuous administration in certain people and the problems of a constipation ameliorating food containing Aloe arborescens extract and chitosan, effective simply for ameliorating constipation, useless for the continuous administration of chitosan and causing side action of aloe such as soft feces and diarrheain certain people, and develop a safe chitosan composition enabling continuous administration of chitosan without causing the constipation side action, free from the side action of diarrhea, etc., by the compounded drug and enabling the administration of the composition for a long period. <P>SOLUTION: The composition solving the above problems contains chitosan in combination with (a) hempseed oil and/or (b) seed of Cassia obtusifolia L. or Cassia tora L. or its extract. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention contains a chitosan blended composition and food, more specifically, (1) chitosan as a blending component, and (2) (a) any one or both of hempseed oil and ketsumeishi or an extract thereof. The present invention relates to a composition and a food product.

Chitosan is a chitin obtained by decalcification treatment with dilute acid and decalcification treatment with dilute alkali on crustacean crust such as crab, shrimp, krill, insect crust like beetle, grasshopper and squid bone. Is a polysaccharide obtained by deacetylation treatment with concentrated alkali. The structure of chitosan is similar to cellulose and hemicellulose, and most of it is quantified as dietary fiber without being digested by digestive enzymes when quantified by the enzyme-weight method (Prosky method), which is a dietary fiber quantification method. Chitosan improves serum cholesterol levels in humans (Biosci. Biotech. Biochem., 57, 1439-1444, (1993), chitin chitosan research, 1 (3), 175-183, (1995), chitin chitosan research, 2 (1), 1-8, (1996), Health and Nutrition Food Research, 2 (1), 27-36, (1999)) and the inhibitory action of intestinal spoilage products (Microbial Ecology in Health and Disease, 8, 15-21, (1995), etc. In recent years, foods for specified health use and many dietary supplements that are expected to have these bioregulatory functions of chitosan have been released. The chitosan has an amino group in its structural formula and has the function of adsorbing various acidic substances such as bile acids, which works with cholestyramine, a cholesterol-lowering drug. The same thing.
Cholestyramine, which has the same action as chitosan in vivo, has side effects of constipation (Aventis Pharma ■, cholestyramine preparation "Questlan" affixed text), but also in chitosan who complains of constipation after ingestion Is seen. If you have symptoms of constipation, take a drink or dairy product containing lactic acid bacteria such as yogurt as a dietary therapy, or ingest vegetables that are rich in dietary fiber, and at the same time activate the intestines by moderate exercise It is important to use a laxative if it is not improved. Even when chitosan is ingested and complains of symptoms of constipation, it is recommended to take these foods at the same time. However, currently available chitosan foods are mostly in the form of tablets and soft capsules that are convenient to carry. It is difficult to take the above-mentioned food for preventing constipation at the same time when chitosan is taken in a scene where chitosan food is actually used. Although it is conceivable to simultaneously take in healthy foods containing dietary fiber and lactic acid bacteria, there are problems such as an increase in the number of grains taken and an increase in the economic burden. Moreover, even in daily exercise, it is difficult to carry out in a modern society where transportation has become IT and mechanized, especially for people living in urban areas.

  In addition, as a food containing a substance with a coughing action in chitosan, by taking aloe and chitosan at the same time to improve the stool properties of constipated people, the swallowing action of aloe improves the constipation, and the chitosan A food for improving constipation has been proposed in which the gastrointestinal tone is adjusted by action to reduce stool odor without diarrhea (see Patent Document 1). However, the food for improving constipation is only for improving constipation, and is not intended for continuous intake of chitosan by non-constipated people. There are fears of causing symptoms, and it is not suitable for continuous intake of chitosan for non-constipated people. For example, in the food for improving constipation, due to the swallowing action of aloin (barbaroin) in Kidachi aloe extract, some people are confirmed to be constipated, and this time people who become mud or watery diarrheal stools are confirmed. Has been. Therefore, there is no known chitosan composition that allows continuous intake of chitosan without causing constipation.

JP 2001-252046 A

  As described above, although chitosan has useful physiological activities such as cholesterol regulating action, as described above, there is no side effect of constipation due to chitosan, and no side effects such as diarrhea due to the drug to be mixed occur. In particular, no safe chitosan preparation or food containing chitosan that can be ingested by chitosan has been found, and development of such a chitosan preparation or food containing chitosan is desired.

Therefore, as a result of various studies, the present inventors have prevented the side effect of constipation due to ingestion of chitosan by simultaneously ingesting chitosan and hemp seed oil and / or ketsumeishi or ketsumeishi extract (hereinafter also simply referred to as an active ingredient). It was found that chitosan can be safely and continuously ingested without causing side effects such as diarrhea due to the combination drug. The present invention has been completed based on the above findings.
That is, the present invention
1. The following ingredients (1) and (2)
(1) Chitosan (2) (a) Hemp seed oil and (b) Ketsumeishi or its extract, either one or both,
A composition comprising:
2. The composition according to claim 1, comprising chitosan, hemp seed oil and ketsumeishi,
3. The composition according to claim 1, comprising chitosan, hemp seed oil and ketsumeishi extract,
4). The composition according to any one of claims 1 to 3, wherein the chitosan is a high-molecular chitosan of 20 mPa · s or more.
5. The composition according to any one of claims 1 to 4, wherein the composition is a liquid, a powder, a granule, a capsule, or a tablet.
6). The following ingredients (1) and (2)
(1) Chitosan (2) (a) Hemp seed oil and (b) Ketsumeishi or its extract, either one or both,
A food characterized by containing,
7). The food according to claim 6, comprising chitosan, hemp seed oil and katsumei
8). 7. The food according to claim 6, comprising chitosan, hemp seed oil and ketsumeishi extract,
9. The food according to any one of claims 6 to 8, wherein the chitosan is a polymeric chitosan having a viscosity of 20 mPa · s or more.
About.

  The composition containing hemp seed oil and / or ketsumeishi or an extract thereof together with chitosan according to the present invention prevents side effects such as diarrhea caused by a combination drug while preventing side effects of constipation due to ingestion of chitosan. The chitosan can be taken safely and continuously.

  The chitosan used in the present invention is usually made from, for example, crustacean crusts such as crabs and shrimp, deproteinized with dilute alkali or enzyme, and decalcified with dilute acid to remove ash. After obtaining chitin, it is obtained by deacetylating chitin in a strong alkali agent such as sodium hydroxide or potassium hydroxide and then washing with water several times, but it is not particularly limited to this method. The chitosan produced is considered to be usable because it has an aggregating effect if it has a viscosity of 5 mPa · s or more (0.5 W / W% 0.5% acetic acid aqueous solution). From the viewpoint, a polymer of 20 mPa · s or more is preferable, and a polymer of 100 mPa · s or more is more preferable. Chitosan used in the present invention can be purchased from chitosan manufacturers such as Nippon Kayaku Food Techno Co., Ltd., Kimika Co., Ltd., Koyo Chemical Co., Ltd., and Dainichi Seika Kogyo Co., Ltd.

  The hemp seed oil (cannabis oil) used in the present invention is not particularly limited as long as it is an edible oil obtained from cannabis (scientific name: Cannabis Sativa L.) seeds. Usually, the oil can be obtained by extracting the remaining oil with a solvent (hexane, which is a food additive) by squeezing the seed mechanically. Moreover, you may extract an oil component with a solvent from the beginning, without pressing. Furthermore, if a low temperature pressing method (cold press) is used, a good quality hemp seed oil with little deterioration of components can be obtained. Moreover, you may refine | purify and use these oils at the following processes. That is, after adding water to the extracted oil and separating the gum, it is removed with a centrifuge, and after obtaining crude oil, phosphoric acid is added to remove the remaining gum, and sodium hydroxide is added to release it. After removing the fatty acid, washing with water, adding activated clay, etc., stirring, adsorbing pigments such as chlorophyll, filtering, removing the clay, and decolorizing. The oil thus obtained is cooled, and after removing the solid fats and waxes that precipitate, the odorous components are removed (deodorized) by steam distillation at high temperature and under vacuum to purify the oil. The purification method is not limited to this method, and a purification method using a column or the like may be used. The amount of hemp seed oil used in the present invention may be in the range of about 1 to 100 parts by mass (hereinafter, parts represent parts by mass unless otherwise specified) with respect to 10 parts by mass of chitosan, Preferably it is 2 parts or more, more preferably 5 parts or more, further 8 parts or more in some cases, and 50 parts or less, more preferably 20 parts or less. A preferred range is 5 to 20 parts. The hemp seed oil used in the present invention can be purchased and obtained from Junsei Sangyo Co., Ltd. (trade name: hemp seed oil), for example.

There is no particular limitation on the ketsumeishi used in the present invention as long as it is a dried mature seed of Ebisugusa (scientific name: Cassia obtusifolia L.) or Cassia tora L. which is a raw material of hub tea. For example, there is no particular limitation as long as it contains an extract extracted from a dried product. For example, an extract, its concentrated extract, a dry powder extract, etc. are mentioned. Usually, those containing an extract obtained by extraction with hot water or cold water are preferred. The dry powder extract is prepared by, for example, pulverizing an extract or concentrate containing the extract by freeze-drying or spray-drying, or adding dextrin, reduced maltose, soluble starch, methylcellulose, etc. to the extract or concentrate containing the extract. An excipient may be added to form a powder.
The amount of Ketsumeishi or its extract used in the present invention may be in the range of about 1 part to 140 parts in terms of Ketsumeishi extract (dry matter) with respect to 3500 parts of chitosan, preferably 10 parts or more, more preferably 20 parts or more, 100 parts or less, more preferably 40 parts or less. Accordingly, the preferred range is 10 to 100 parts, more preferably 20 to 40 parts. Ketsumeishi used in the present invention can be purchased and obtained from, for example, Maruzen Pharmaceutical Co., Ltd. (product name: Seimeiko Extract Powder MF).

The composition of the present invention may contain at least one of hemp seed oil or ketsumeishi or an extract thereof together with chitosan in a composition. In a more preferred embodiment, the composition of hemp seed oil and ketsumeishi or an extract thereof is included. It includes both. In the composition containing both hemp seed oil and ketmeishi or its extract together with chitosan, the ratio of hemp seed oil and ketmeishi or its extract to chitosan may be the ratio described above.
The composition of the present invention may be composed of only active ingredients, but is usually a composition formulated with additives such as excipients, or a food containing the active ingredients.

 Examples of the formulated composition of the present invention include those formulated with additives such as excipients by conventional methods such as liquids such as drinks, powders, granules, capsules and tablets. In addition, the food is not particularly limited, and usually includes noodles, breads, ham, sausage, processed meat products such as kamaboko or hamburger, side dishes, confections such as biscuits, drinks such as juices, etc. Any food may be used. When chitosan is added to food or the like, chitosan is usually added in the form of powder, flakes, or a solution dissolved in an organic acid aqueous solution. In addition to the above active ingredients, the composition includes fillers such as lactose, starch, edible eggshell powder, dolomite, binders such as methylcellulose, soluble starch and gelatin, disintegrants such as cellulose, ground wheat bran and agar, Lubricants such as glycerin fatty acid esters, talc and magnesium stearate, coating bases such as shellac and methylcellulose, acidulants such as citric acid, malic acid and vitamin C, sucrose, fructose, reduced maltose, sorbitol, aspartame, etc. Additives for preparations such as sweeteners and fragrances and / or food materials can be included as necessary. The content in these compositions is the remainder excluding the content of chitosan, hemp seed oil, ketmeishi or its extract, but the amount is usually 0 to 99.9% in the composition (mass: the same below) Range. Therefore, the active ingredient content in the composition of the present invention is 100 to 0.1%. Chitosan may be added in the form of powder, flakes or an organic acid aqueous solution.

The active ingredient content in the composition of the present invention is not particularly limited as long as it is in the above range, but the daily intake of chitosan is included so that it can be ingested by single or multiple ingestion. It is preferable. The daily intake requirement varies depending on the purpose, etc., so it is difficult to say generally. For example, about 10 mg to 9 g / adult, preferably about 100 mg to 5 g / adult, more preferably about 500 mg to 2 g / adult It is preferable that it is contained so that it can be ingested.
In the case of formulated supplements such as health foods, for example, chitosan in the solid preparation is 5 to 80%, preferably about 10 to 50%, and in some cases 20 to 45%. %. Other active ingredients may be included in the above-described ratio range based on chitosan. In the case of a capsule preparation, the above ratio is the composition ratio of the contents filled in the capsule. In addition, in the case of food, etc., the chitosan content is usually 0.1% or more, preferably 0.2% or more in the food, and the upper limit is in the same range as above, taking the intake, taste, etc. into consideration. It is determined.

Test Examples The present invention will be specifically described below with reference to test examples, but the present invention is not limited to these examples.
(1) Manufacture of test soft capsules In order to confirm the effects of the present invention, in conducting a human intake test, four types of soft capsules were manufactured as shown in Table 1 below. The control food contains chitosan only, the prescription 1 contains ketmeishi extract in the control, the prescription 2 contains hemp seed oil in the control, and the prescription 3 contains both ketmeishi extract and hemp seed oil in the control Are blended. Each formulation was uniformly dispersed and mixed, excluding the capsule raw material, and filled into soft capsules so that the inner volume was 300 mg.

(2) Test method Randomly elected healthy men in their 20s to 50s, 16 women, and a total of 24 monitors, divided into 4 groups so that there is no bias in gender and age. After taking 10 capsules of the control capsule preparation per day for each group for one week, the control capsule preparation was continued in group 1, the capsule preparation of prescription 1 in group 2, the capsule preparation of prescription 2 in group 3, Group 4 was given 10 capsules per day for 21 days, respectively. In taking capsules, the daily intake of 10 capsules was taken in water or hot water at any time, but it was decided that 10 capsules could be taken together or in several divided doses. The change in physical condition before and after ingestion was investigated by questionnaires filled in by the person six times before ingestion, 7 days after ingestion of the control capsule preparation, 7 days after ingestion of each test capsule preparation, 14 days, 21 days, and 7 days after discontinuation of intake. The study was conducted in accordance with the spirit of the Declaration of Helsinki, and the volunteers were fully explained about the purpose, content, and method of the study, and consent was obtained from the study.

(3) Questionnaire items Questionnaire items were scored and entered according to the evaluation criteria shown in Table 2 below.

(4) Test results The stool volume / fecal score and the number of defecations are shown in Table 3 below. When taking a control capsule formulation containing only chitosan for one week, the stool volume score increases in all groups, but the stool is hard and the number of defecations tends to decrease, indicating constipation symptoms. ing. However, after taking the test capsule preparation for 3 weeks, the tendency of constipation is not improved in the control due to the convenience and the number of defecations, but the formula 1 with Ketsumeishi extract added to chitosan and hemp seed oil added to chitosan In the prescription 2, until 7 days after ingestion, the stool property and the number of defecations are almost restored to the state before ingestion. However, as the test meal continued to be consumed, constipation and the number of bowel movements gradually showed constipation. Therefore, although the prescription 1 and prescription 2 have a temporary effect, ingesting chitosan for a long period of time may require an interruption of the intake, and there is room for further improvement. On the other hand, in Group 4 who took the capsule formulation of Formula 3 with Ketsumeishi extract and hemp seed oil added to chitosan, the stool properties and the number of defecations remained the same as before, and side effects of constipation due to chitosan were suppressed In addition, there is no side effect such as muddy feces due to the blended hemp seed oil and / or ketsumeishi extract, and it is shown that the effect is sustained over a long period of time. That is, this test result shows that a certain effect is observed even in a preparation containing two kinds of chitosan and ketsumeishi extract or two kinds of chitosan and hemp seed oil. It is shown that a composition containing both hemp seed oil and ketsumeishi or an extract thereof with chitosan is the most suitable for the continuous intake of.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

  Mix 20.9kg of chitosan, 20.9kg of crystalline lactose, 6.28kg of crystalline cellulose, 10.47kg of hemp seed oil, 0.146kg of ketsumeishi extract and 1.19kg of methylcellulose, mix with 20kg of water, knead, dry and adjust with a speed mill. Granulated to obtain chitosan granules.

  Stir 1 g of chitosan, 400 g of wheat flour (medium flour), 0.5 g of hemp seed oil, 0.5 g of ketsumeishi extract (2% product) powder and 5 g of salt with a raikai machine, then add 200 ml of water and further stir the dough. Then, after rolling with a noodle making machine for 12 hours, it was cut out to obtain chitosan udon.

  Chitosan 23.3kg, Ketsumeishi extract (2% product) 10.0kg, Hemp seed oil 33.3kg, Wheat germ oil 20.0kg, Vitamin B 21.7kg, Vitamin B 61.3kg, Vitamin E (67% product) 2.0kg, Beeswax 6.7kg Then, 1.7 kg of glycerin fatty acid ester was mixed, and 300 mg of the mixture was filled into soft capsules to obtain chitosan granules.

  Mixing chitosan 1.0g, wheat flour 200.0g, butter 70.0g, hemp seed oil 1.0g, ketsumeishi extract 0.01g, sucrose 80.0g, egg 50.0g, cutting after rolling, baking in a 200 ° C oven and hardened with chitosan I got a biscuit.

Chitosan 8.0g, ground pork 1000.0g, onion (chopped) 150.0g, hemp seed oil 8.0g, ketsumeishi extract 0.08g, salt 20.0g, sugar 4.0g, pepper 3.5g, seasoning spice 10.0g, water 120.0g Then, after standing at 4 ° C for 3 hours, it was stuffed into salted pork intestines, shaped to about 80g per bottle, and overheated in hot water at 80 ° C for 20 minutes to obtain sausage with chitosan.

Claims (9)

The following ingredients (1) and (2)
(1) Chitosan (2) (a) Hemp seed oil and (b) Ketsumeishi or its extract, either one or both,
The composition characterized by containing. The composition according to claim 1, comprising chitosan, hemp seed oil and ketsumeishi. The composition according to claim 1, comprising chitosan, hemp seed oil and ketsumeishi extract. The composition according to any one of claims 1 to 3, wherein the chitosan is a polymeric chitosan having a viscosity of 20 mPa · s or more. The composition according to any one of claims 1 to 4, wherein the composition is a liquid, a powder, a granule, a capsule or a tablet. The following ingredients (1) and (2)
(1) Chitosan (2) (a) Hemp seed oil and (b) Ketsumeishi or its extract, either one or both,
A food product characterized by containing. The food according to claim 6, comprising chitosan, hemp seed oil and katsumeshi. The food according to claim 6, comprising chitosan, hemp seed oil and ketsumeishi extract. The food according to any one of claims 6 to 8, wherein the chitosan is a polymeric chitosan having a viscosity of 20 mPa · s or more.