JP2006306848A - Copper compound-incorporated composition for liquid preparation for internal use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a copper compound-incorporated composition for liquid preparation for internal use reduced in copper ion-derived astringency, easy to drink, and also leaving unpleasant aftertaste. <P>SOLUTION: The composition for liquid preparation for internal use is obtained by incorporating a copper compound and specific amino acid(s)(one or more kinds selected from the group consisting of acidic amino acid and amide thereof, basic amino acid and γ-aminobutyric acid and salts thereof). <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  [Technical Field] The present invention relates to a composition for internal use of a copper compound that reduces the unpleasant taste of a copper compound and improves the feeling of taking, and can be used in the fields of medicine and food.

  An internal liquid preparation containing a copper compound was difficult to drink due to an unpleasant taste derived from copper ions. This taste is the same as the taste when using an astringent that binds to proteins such as tannin and alum.

  Until now, in order to improve the unpleasant taste of a copper compound, the technique of mix | blending an oleoresin and an accent fragrance | flavor is disclosed (patent document 1).

  However, these are not sufficient in improving the unpleasant taste derived from copper ions.

Patent No. 2580648

  An object of the present invention is to provide a composition for internal use of a copper compound that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  In order to solve the above-mentioned problems, the present inventors have made extensive studies, and as a result, the copper compound is selected from the group consisting of acidic amino acids and amides thereof, basic amino acids, γ-aminobutyric acid and salts thereof. Or it discovered that the astringent taste by a copper ion was improved by mix | blending 2 or more types of amino acids (henceforth a specific amino acid), and completed this invention.

The present invention has been completed based on such findings, and includes the following inventions.
(1) A copper compound and an acidic amino acid and its amide, a basic amino acid, γ-aminoacetic acid, and one or more amino acids selected from the group consisting of these salts are blended, and the pH is 2.5. The composition for internal use liquid characterized by being -7.0.
(2) The copper compound is one or more compounds selected from the group consisting of copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate, and copper phosphate. Composition for internal use.
(3) An oral liquid composition comprising one or more amino acids selected from the group consisting of acidic amino acids and amides thereof, basic amino acids, γ-aminoacetic acid and salts thereof. How to reduce unpleasant taste.

  INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a composition for internal use liquid that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  This composition for internal use liquid can be applied to, for example, various preparations including pharmaceuticals such as syrups and drinks and quasi-drugs, and various beverages such as nutritional functional foods.

  The copper compound in the present invention is a salt with an organic acid or inorganic acid containing copper as a cation component, and these organic acid and inorganic acid may be non-toxic, and examples of the organic acid include gluconic acid. Citric acid, tartaric acid, or inorganic acids include, for example, sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, and carbonic acid.

  Examples of the copper compound to be blended include copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate, and copper phosphate. These may be blended singly or in combination of two or more.

  The compounding quantity of a copper compound changes with the intended purpose of the composition for internal use liquid which mix | blends this. In terms of nutritional intake, 0.1 to 10 mg per day is preferable in terms of copper ions. When taken as a 100 mL oral liquid solution per day, the copper ion concentration is 0.0001 to 0.01 W. / V%.

  In the present invention, acidic amino acids (aspartic acid, glutamic acid, etc.) and their amides (asparagine, glutamine, etc.), basic amino acids (arginine, histidine, lysine, etc.), γ-aminobutyric acid and salts thereof (hereinafter referred to as “specific” as appropriate) A desired astringent taste improving effect can be obtained by using the “amino acids”.

  The amino acid salts include salts of amino acids with acids (hydrochloric acid, sulfuric acid, methanesulfonic acid, etc.), bases (ammonia, sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide, etc.) or other amino acids. Can be mentioned.

  In the present invention, one or more of these specific amino acids can be selected and blended.

  The γ-aminobutyric acid used in the present invention is not limited to synthetic products, but is also derived from plants such as rice germ, rice bran, tea and nanban, and those derived from microorganisms such as koji mold, lactic acid bacteria, yeast and chlorella. be able to.

  If the compounding ratio of the copper compound and the specific amino acids is 0.1 parts by mass or more with respect to 1 part by mass of the copper ions, the desired effect can be obtained, but preferably 1 to 20000 parts by mass, Preferably it is 5-10000 mass parts.

  Although the desired effect can be obtained by adjusting the pH of the composition for internal use liquid of the present invention to 2.5 to 7.0, it is adjusted to pH 3.0 to 5.5 for ease of drinking. It is preferable.

  The pH of the composition for internal use liquid includes, for example, organic acids such as citric acid, malic acid, fumaric acid, tartaric acid, lactic acid and succinic acid, salts of these organic acids, inorganic acids such as phosphoric acid and hydrochloric acid, sodium hydroxide It can adjust by adding inorganic bases, such as.

  In the composition for internal use liquid containing the copper compound of the present invention, vitamins, minerals, other amino acids, herbal medicines, herbal extracts, caffeine, royal jelly, etc. are appropriately selected within a range not impairing the effects of the present invention. Can be blended. Moreover, additives such as antioxidants, colorants, flavors, flavoring agents, surfactants, solubilizers, preservatives, sweeteners, and the like can be appropriately blended as necessary so long as the effects of the present invention are not impaired.

  The method of preparing the composition for internal use liquid which mix | blended the copper compound of this invention is not specifically limited. Usually, after dissolving each component with an appropriate amount of purified water, adjust the pH, adjust the volume by adding the remaining purified water, and filter and sterilize as needed to mix the desired copper compound. The composition for use is obtained.

  Hereinafter, the present invention will be described in more detail with reference to examples and test examples. An example is an example which shows an embodiment of the invention concretely, and a test example is an example of a test which evaluated an example. The present invention is not limited to these examples.

Example 1
Copper gluconate 0.004g
Zinc gluconate 0.02g
Arginine hydrochloride 0.20g
0.25 g of magnesium aspartate
Calcium gluconate 0.50g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 2.00g
Xylitol 4.00g
Trehalose 5.00g
Erythritol 5.00g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 2
Copper gluconate 0.007g
Zinc gluconate 0.05g
Histidine hydrochloride 0.10g
Arginine hydrochloride 0.20g
Calcium gluconate 0.40g
Magnesium aspartate 0.30 g
0.01 g of thiamine nitrate
Riboflavin 0.01g
0.10 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 1.00g
Sorbitol 4.00g
Trehalose 5.00g
Xylitol 4.00g
Stevia extract 0.03g
Acesulfame potassium 0.03g
Malic acid 0.10g
Citric acid 0.40g
Sodium citrate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
Apple flavor 0.10g
After dissolving the above components in purified water, the pH was adjusted to 4.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 3
0.03 g of copper gluconate
Zinc gluconate 0.08g
γ-aminobutyric acid 0.20 g
Arginine hydrochloride 0.20g
Riboflavin 0.01g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Cyanocobalamin 120μg
Panthenol 0.01g
Nicotinamide 0.05g
Aminoethylsulfonic acid 1.00g
Sorbitol 5.00g
Trehalose 2.00g
Maltitol 2.00g
Citric acid 0.40g
Sodium malate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 4.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 4
Copper gluconate 0.007g
Zinc gluconate 0.05g
Histidine hydrochloride 0.10g
Arginine hydrochloride 0.20g
0.03 g of ammonium iron citrate
Calcium gluconate 0.80 g
Magnesium aspartate 0.40 g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Nicotinamide 0.10g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Glucose 5.00g
Indigestible dextrin 4.00 g
Erythritol 5.00g
Xylitol 2.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Sucralose 0.005g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 5
0.03 g of copper gluconate
Zinc gluconate 0.08g
γ-aminobutyric acid 0.20 g
Glutamine 0.20g
Arginine hydrochloride 0.20g
Histidine hydrochloride 0.10g
0.03 g of ammonium iron citrate
Calcium gluconate 0.20g
Calcium lactate 0.20g
Sodium aspartate 0.10g
Magnesium aspartate 0.30 g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.02g
0.03 g of pyridoxine hydrochloride
Nicotinamide 0.05g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Royal jelly 0.60g
Glucose 5.00g
Sorbitol 5.00g
Xylitol 5.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Citric acid 0.60g
Sodium citrate 0.10g
Phosphoric acid 0.30 g
Hydrochloric acid appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Test example
Hagerman et al. Reported that protein (bovine serum albumin: BSA) in solution was aggregated by tannin, and the amount of precipitation was proportional to the amount of tannin ( J. Agric . Food . Chem ., 1978, Vol. 26 , 809) . -812). The present inventors have found that when a BSA solution is added to a copper ion solution, the solution is suspended, the amount of light transmitted decreases, and the decrease in the amount of transmission correlates with the concentration of copper ions.

Test method (1) Preparation of BSA solution: 6 g of BSA (Sigma Chemical Co .; fraction V, fatty acid free) is dissolved in an appropriate amount of purified water, 100 mg of citric acid is added, and the pH is adjusted with NaOH solution (1 mol / L). Adjusted to 4.8 and made up to 100 mL with purified water.

  (2) Preparation of diluting solution: 100 mg of citric acid was dissolved in an appropriate amount of water, pH was adjusted to 4.8 with NaOH solution (1 mol / L), and purified water was adjusted to 100 mL.

  (3) Preparation of copper ion solution: 0.10 g of citric acid was added to 0.02 g, 0.11 g and 0.18 g of copper gluconate. Each was dissolved in an appropriate amount of purified water, adjusted to pH 4.8 with NaOH solution (1 mol / L), and made up to 100 mL with purified water.

(4) Evaluation of protein-copper ion interaction (convergence) 6 mL of BSA solution was added to 2 mL of each copper ion solution, and the total volume was adjusted to 10 mL with a diluted solution. This was shaken at 40 ° C. for 30 minutes. Using a quartz cell (L = 1 cm), the absorbance at 500 nm, which is a wavelength that is not absorbed by each transparent solution, was measured with a spectrophotometer (manufactured by Hitachi, Ltd .: U-3300). This result (FIG. 1) shows that the copper ion concentration and the absorbance are correlated.

  It was confirmed that the results of sensory evaluation of the astringent taste of copper ion solutions having various concentrations were correlated with the absorbance when the BSA solution was added to the copper ion solution. The sensory evaluation was performed as B when the astringent taste was strongly unacceptable, A4 to A1 according to the strength of the astringent taste, and A when the astringent taste was not felt at all. The results are shown in FIG.

  From these facts, when the absorbance when a copper ion solution added with a certain substance is mixed with a BSA solution decreases compared to the case where no addition is made, the addition of that substance causes the aggregation (convergence) due to protein-copper ion interaction. ) Is reduced, that is, the astringency is reduced.

(5) Protein Aggregation Properties of Various Amino Acids After preparing various amino acid solutions shown in Table 1, the protein aggregation properties of various amino acids were evaluated by the methods described in the above test methods (1) to (4). The results are shown in Table 2. From Table 2 and FIG. 2, it is shown that acidic amino acids and their amides, basic amino acids, and γ-aminobutyric acid have an excellent inhibitory effect on protein aggregation, and their absorbance is in a range where almost no astringency is felt. It was done. Furthermore, BSA solution-extracted specimens were actually prepared for acidic amino acids and their amides, basic amino acids, and γ-aminobutyric acid according to the method described in the above test method (4). The astringent taste was evaluated and the results are shown in Table 3 below. As is apparent from Table 3, acidic amino acids and their amides, basic amino acids, and γ-aminobutyric acid were easier to drink and did not leave an unpleasant aftertaste as compared to the case of no addition.

FIG. 1 shows the correlation between protein aggregation and copper ion concentration. FIG. 2 shows the correlation between absorbance and sensory evaluation.

According to the present invention, since it is possible to drink without feeling unpleasant taste of the copper compound despite being a composition for internal use liquid containing a copper compound, for example, syrups, drinks, etc. It is possible to provide various beverages such as various internal liquid solutions and nutritional functional foods including pharmaceuticals and quasi drugs.

Claims (3)

  The copper compound is blended with one or more amino acids selected from the group consisting of acidic amino acids and their amides, basic amino acids, γ-aminoacetic acid and salts thereof, and the pH is 2.5-7. The composition for internal use liquid characterized by being zero.   The composition for internal use according to claim 1, wherein the copper compound is one or more compounds selected from the group consisting of copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate and copper phosphate. object. Discomfort of composition for internal use of copper compound, characterized in that it contains one or more amino acids selected from the group consisting of acidic amino acids and amides thereof, basic amino acids, γ-aminoacetic acid and salts thereof. Taste reduction method.

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