JP2006266875A - Simplified electrophoretic device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problems wherein a conventional electrophoretic device using a cellulose acetate film as a support has a large size and requires an expensive power supply device, and electrophoresis preparation or care after finish of an electrophoretic vessel such as exchange of a bridge material, exchange of buffer solution, or cleaning of the electrophoretic vessel is troublesome. <P>SOLUTION: In this simplified electrophoretic device for the cellulose acetate film, when a lid of the electrophoretic vessel is closed, the cellulose acetate film forms an arch shape simultaneously by the lid, and the film ends are dipped respectively into the buffer solution in a cathode side vessel and in an anode side vessel, to thereby dispense with the bridge material into which the buffer solution is penetrated. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a simplified electrophoresis apparatus used for preparing a fraction pattern of protein or DNA in cellulose acetate membrane electrophoresis analysis.

Conventionally, fractional analysis by electrophoresis of in-vivo substances such as serum and urine has been used all over the world as a useful means for obtaining various disease information from a single drop of specimen.

Among these, cellulose acetate membranes have been widely used as a support for electrophoresis because they are convenient to handle such as being stored at room temperature and dried.

In the cellulose acetate membrane electrophoresis analysis method, after the cellulose acetate membrane is once wetted with a buffer solution, the sample is applied and then loaded into the electrophoresis tank and then energized to form a fractionation pattern by electrophoresis. Then, the dyed solution is stained with a staining solution, and unnecessary portions are decolored, and then measured and quantified with a densitometer.

The reason for wetting with the buffer solution is that the specimen to be applied is uniformly sucked into the support, and various ions contained in the specimen can move quickly through the support during energization.

Therefore, if wetting is performed using a buffer solution having a high degree of fatigue and containing a large amount of impurity ions, movement of ions to be measured contained in the specimen is hindered, resulting in a problem that the electrophoretic image is distorted.

In addition, the buffer solution used in the electrophoresis tank is used to compensate for the buffer solution evaporating from the support while power is applied, and a bridge material wetted from the periphery of the support is used. Supplied.

As a conventional simplified electrophoresis apparatus, an electrophoresis apparatus disclosed in Japanese Utility Model Publication No. 63-39639 is known. In this electrophoresis apparatus, various predetermined gel trays and boxes are appropriately selected and used using agarose gel or polyacrylamide gel as a support.
Shoko Sho 63-39639

As described in the background art, a simple electrophoresis apparatus using an agarose gel or a polyacrylamide gel as a support has been present and has become an important experimental instrument in the technical field.

On the other hand, an electrophoresis apparatus using a cellulose acetate membrane as a support has been conventionally large and requires an expensive power supply apparatus.

Preparation of the electrophoresis tank and maintenance after the completion, such as replacement of the bridge material, buffer solution, and washing of the electrophoresis tank, were complicated.

The present invention is a simple type in which a rectified alternating current is passed through a cellulose acetate membrane and electrical connection can be interrupted when an overcurrent or abnormal current flows, and an expensive power supply device is not required separately from the electrophoresis device. This is an electrophoresis apparatus for cellulose acetate membrane.

When the lid of the electrophoresis tank is closed, the cellulose acetate membrane becomes arched by the lid at the same time, and the membrane end is immersed in the buffer solution of the cathode side tank and the anode side tank, respectively, so that no bridge material infiltrated with the buffer solution is required. This is a simple cellulose acetate membrane electrophoresis apparatus that allows easy buffer exchange and easy maintenance.

This is a simple cellulose acetate membrane electrophoresis apparatus having a structure that cannot be energized without a lid.

Electrophoresis using a cellulose acetate membrane as a support is possible without requiring special equipment.

Electrophoresis using a cellulose acetate membrane as a support is possible without troublesome operations such as preparation of a bridge material, washing of an electrophoresis tank, and setting of a support.

A sample is applied to a cellulose acetate membrane wetted with a buffer solution, and then set in a simple electrophoresis apparatus. The electrophoresis tank is covered and the cellulose acetate membrane is arched. At the same time, the electrical connection is closed and the electrophoresis is started. The electrophoresis is completed in about 30 minutes.

1 and 2 are diagrams showing a configuration example of the simple electrophoresis apparatus of the present invention, and this example will be described in detail.

Plug outlet 1 into a commercial AC power source.

The buffer solution is put into the electrophoresis tank cathode side 2 and the electrophoresis tank anode side 3.

The cellulose acetate film 4 wetted with a buffer solution and coated with the specimen is set in the electrophoresis tank 5.

Close the lid 6 of the electrophoresis tank and start electrophoresis.

After the electrophoresis is completed, the lid 6 of the electrophoresis tank is opened and the cellulose acetate film 4 is taken out.

Discard the used buffer.

The electrophoresis tank 5 is lightly washed and dried. The lid 6 of the electrophoresis tank is washed in the same manner.

In addition, this invention is not limited to the said embodiment. For example, a separate power switch may be attached to the main body of the electrophoresis tank, and the electrophoresis switch may be started by operating the power switch after closing the lid 6 of the electrophoresis tank.

It is a figure before attaching the lid | cover of the electrophoresis tank which shows the example of a structure of this invention. It is a figure after attaching the lid | cover of the electrophoresis tank which shows the example of a structure of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Outlet 2 Electrophoresis tank cathode side 3 Electrophoresis tank anode side 4 Cellulose acetate membrane 5 Electrophoresis tank 6 Electrophoresis tank cover

Claims (1)

In a cellulose acetate membrane electrophoresis apparatus that can form a fractionation pattern by electrophoresis after applying a sample to a cellulose acetate membrane that has been wetted with a buffer solution, simultaneously with closing the lid of the electrophoresis tank. A simple electrophoresis apparatus having a structure in which a cellulose acetate film is formed into an arch shape by a lid, and a membrane material is immersed in a buffer solution of a cathode side tank and an anode side tank, so that a bridge material is not required.

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