JP2006239393A - Allergen inactivating agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an effective allergen inactivating agent with no odor or color. <P>SOLUTION: An aqueous material comprising a water-soluble zinc compound or a water-insoluble zinc compound, or zinc/metal oxide composite material, is used. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

The present invention relates to a drug that adsorbs or immunologically inactivates a protein allergen that is difficult to remove by conventional methods and a method for using the same.

  Conventionally, allergic diseases that become sensitized by continuously inhaling, contacting or ingesting vegetable proteins such as cedar pollen, animal proteins such as mite worms and excrement, and livestock hair have become a problem. ing. Once the sensitized state is obtained in this way, a remarkably allergic reaction such as dermatitis, rhinitis, and asthma is caused by re-ingestion of a trace amount of the same allergen by inhalation, contact, etc. Turn into. In order to suppress such an allergic reaction, it is important to avoid contact with the causative substance allergen, and many studies have been made.

As a means for avoiding contact with allergens, for example, a method of physically removing floor accumulated dust or airborne dust with a vacuum cleaner or an air cleaner to reduce allergens is recommended. However, it is difficult to completely remove the finely divided particulate matter, which may cause re-scattering in some cases. Moreover, curtains, futons, air purification filters, etc. are difficult to remove effectively due to restrictions on the shape and installation location.

  Also disclosed is a method of spraying, spraying, or applying a drug having allergen inactivating ability in the environment.

  For example, an antiallergen agent that inactivates allergens present in the environment by utilizing the protein binding action of a tannic acid compound, and a method for using the same (for example, see Patent Documents 1 and 2), and a tea extract containing the above components The filter etc. which have allergen inactivation property by apply | coating (for example, refer patent document 3) are disclosed.

  However, (1) Tannic acid and polyphenols having a catechol-like structure are likely to be discolored by oxidation or chelate formation with coexisting metal ions. (2) In the case of a crude extract with a low degree of purification, contamination with impurities Odor generation is unavoidable, and (3) since it is substantially water-soluble, re-elution occurs due to contact with the coating substance.

JP-A 61-44821 Japanese Patent Laid-Open No. 06-279273 JP 2000-5553 A

  The present invention has been made against the background of the problems of the prior art. An object of the present invention is to provide an odorless and colorless allergen inactivating agent while having excellent allergen inactivating ability.

  As a result of intensive studies to solve the above problems, the present inventors have found that a zinc-based compound is effective as an allergen-inactivating component, and finally completed the present invention. That is, the present invention is (1) an aqueous allergen inactivating agent characterized by containing a water-soluble zinc compound, (2) a water-insoluble zinc compound and / or a water-insoluble zinc / metal oxide composite particle An allergen inactivating agent characterized by being dispersed in water, (3) an allergen inactivating agent according to (2), wherein the particle size is 50 μm or less, and (4) a metal oxide is titania The allergen inactivating agent according to (2) or (3), or the allergen inactivating agent according to any one of (5) (1) to (4), comprising at least one of silica, silica, and It is a filter having antiallergenic properties that is carried.

The allergen inactivating agent according to the present invention has the advantage that, by using an odorless and colorless zinc compound, excellent properties that could not be achieved in the past can be obtained and can be developed for a wider range of applications.

Hereinafter, the present invention will be described in detail.
The allergen inactivating agent according to the present invention preferably contains a water-soluble zinc compound. Zinc-based compounds have an excellent allergen inactivating effect and are substantially colorless and odorless, so they can eliminate the problems of coloring, discoloration, and odor, and further exist in the aqueous solution as ions. This is because the property is extremely good. Although the water-soluble zinc compound used for this invention is not specifically limited, For example, zinc nitrate, zinc sulfate, etc. can be illustrated.
The concentration of the water-soluble zinc compound in the allergen inactivating agent according to the present invention is preferably 0.005 mol / L to 3 mol / L. When the amount is less than 0.005 mol / L, a sufficient effect cannot be obtained. On the other hand, when the amount exceeds mol / L, the effect hardly changes and the cost increases. More preferably, they are 0.05 mol / L-1 mol / L, More preferably, they are 0.1 mol / L-0.5 mol / L.

The allergen inactivating agent according to the present invention may be one in which water-insoluble zinc compound particles are dispersed in water. This is because protein allergens can be inactivated by spraying and applying such a liquid to the environment or contaminated articles. The zinc compound used in the present invention is not particularly limited, and examples thereof include metal zinc and zinc oxide. The content of the water-insoluble zinc compound particles is preferably 0.1% by weight to 30% by weight. If the amount is less than 0.1% by weight, a sufficient effect cannot be obtained. On the other hand, if the amount exceeds 30% by weight, particle dispersibility (reaggregation) and clogging problems occur when used in a spray. . More preferably, it is 0.5 weight%-20 weight%, More preferably, it is 1 weight%-15 weight%.

The allergen inactivating agent according to the present invention may be a carrier made of a metal oxide different from water-insoluble zinc / metal oxide composite particles, or may be mixed with the water-insoluble zinc. As a form of the composite particles, a zinc compound is supported on the surface layer or a part thereof. This is because the amount of zinc element used can be reduced. Examples of preferred metal oxides include alumina, silica, titania and the like.

The particle diameter of the water-insoluble zinc compound particles and the water-insoluble zinc / metal oxide composite particles used in the present invention is preferably 50 μm or less. This is because within such a range, the handleability in the spraying or coating stage can be improved, and the appearance change of the deposit can be suppressed. More preferably, it is 100 nm or less, and most preferably 10 nm or less. In addition, by reducing the particle size, the surface area is dramatically increased, so that the inactivation effect is enhanced, and there is also an advantage that scattering after coating can be suppressed by improving adhesion. In particular, when the particle diameter is 10 nm or less, a film can be formed by drying.

The lower limit of the particle diameter of the water-insoluble zinc compound particles used in the present invention is not particularly limited, but is preferably 1 nm or more from the viewpoint of the handleability and dispersion stability of the powder.

Since the allergen inactivating agent of the present invention is intended for use in the living environment, it is preferably dissolved or dispersed in a solvent mainly composed of water from the viewpoint of inhalation toxicity, flammability, etc. A complete aqueous solvent is preferred. However, it does not preclude the addition of a low-boiling solvent such as alcohol, an antibacterial agent, a deodorizing agent, or a surfactant as an auxiliary agent as necessary.

  Further, the pH of the allergen inactivating agent is preferably in the range of 3 to 11 in view of safety, more preferably 5 to 9, and most preferably 6 to 8. Further, insolubility in the present invention means insoluble in the pH of an aqueous solution used as a dispersion medium.

  In addition, the allergen inactivating agent of the present invention is not only used for contaminated environments and articles, but also for building components such as floors and walls, air conditioners, textile products such as woven fabrics and nonwoven fabrics, masks, filters, etc. It is also preferable to apply, spray, and carry the filter member in advance.

  The allergen applicable in the present invention is not particularly limited as long as the inactivation effect can be confirmed, but specifically, derived from domestic animals such as dogs, cats and bird hairs, derived from plants such as cedar and ragweed, mites and cockroaches. Examples of animal and plant proteins such as body and excrement can be given.

The present invention will be specifically described below with reference to examples.

(Adjustment of derf2 allergen test solution)
100 mg of Mite-Dermatophhgolides farinae was collected from LSL and ground using an agate mortar. The obtained pasty substance was stirred and dispersed in 5 mL of 0.05% PBS-T (0.05% Tween 20-containing PBS pH 7.4), and allowed to stand at 4 ° C. for 18 hours. Particulate matter was removed using a syringe membrane filter having a hole diameter of 0.45 μm to prepare a Derf2 allergen test solution, which was used in the following experiments.

(Measurement of optimum concentration of derf2 allergen test solution)
In carrying out the test, a Derf2 titer test on mite extracts was carried out. For detection of Derf2, a simple tick detection kit Taniscan (trademark) manufactured by Asahi Breweries Co., Ltd. was used, and the above-mentioned extract and 10-fold and 100-fold diluted solutions with PBS-T (pH 7.4) were adjusted, and a micropipette was used. 150 μL was added dropwise.
Since the result of concentration labeling (3: C = T) was obtained with a 100-fold diluted product, in subsequent tests, it was decided to use it at a 100-fold diluted concentration in the detection kit inoculation stage.

Example 1
ZSI was collected in a hydrophobic microtube (volume: 1.5 mL) manufactured by SSI, and 100 μL of PBS-T was added to make a 5 w% aqueous solution. Subsequently, 100 μL of the mite extraction stock solution was dispensed and stirred.
The sample was left in the dark at 25 ° C. for 22 hours, diluted 50-fold with PBS-T, and 150 μL was inoculated into the detection kit. A result of “1: No contamination” was obtained, and it was confirmed that the Derf2 allergen was immunologically inactivated or adsorbed. The test solution was applied and dried on a trade name: Kimwipe manufactured by Crecia Co., Ltd., but it was colorless.

(Example 2)
SSI Hydrophobic Microtube (capacity 1.5mL) and Catalytic Chemical Industry Co., Ltd. ATOMY B
900 mL of ALL-TZR and then 100 μL of mite extraction stock solution were injected and mixed. After leaving it in the dark at 25 ° C. for 22 hours, it was diluted 10-fold with PBS-T, of which 150 μL was inoculated into the detection kit. A result of “1: No contamination” was obtained, and it was confirmed that the Derf2 allergen was immunologically inactivated or adsorbed. The test solution was applied and dried on a trade name: Kimwipe manufactured by Crecia Co., Ltd., but it was colorless.

(Comparative Example 1)
900 ml of PBS-T solution was dispensed into an SSI hydrophobic microtube (volume 1.5 ml), and then 100 μL of a tick extraction stock solution was injected and mixed. After standing in the dark at 25 ° C for 22 hours, diluted 10-fold with PBS-T. Of these, 150 μL was inoculated into the detection kit, resulting in “3: Contaminated”, confirming that the Derf2 allergen was not immunologically affected or adsorbed. The test solution was applied to Crecia Co., Ltd. Product name: Kimwipe and dried, but it was colorless.

(Comparative Example 2)
0.08 g of tea extract (Shirai Matsushin Pharmaceutical Co., Ltd., catechin 60W) was dissolved in PBS-T to make a 10% solution.
100 μL of green tea extract and mite extraction stock solution were injected and mixed in an SSI hydrophobic microtube (volume: 1.5 mL). The mixture was left at 25 ° C. for 22 hours and diluted 50-fold with PBS-T. As a result of inoculating 150 μL of the detection kit, “1: No contamination”,
It was confirmed that the derf2 allergen was immunologically inactivated or adsorbed. However, the test solution was applied to Crecia Co., Ltd., trade name: Kimwipe Kimwipe, and as a result, it turned brown and had a tea-specific odor.

(Example 3)
Catalytic Chemical Industry Co., Ltd. ATOMY BALL-TZR was supported on a 1 cm square polyester non-woven fabric (weight per unit: 60 g / m 2) corresponding to 0.015 mL to prepare a filter having antiallergenic properties. This filter is immersed in 1 mL of PBS-T containing 50 ng / mL of purified mite antigen (Der f2) (manufactured by Asahi Food and Healthcare Co., Ltd.) and allowed to stand at room temperature for 24 hours. Measured. As a result, the Derf2 allergen was inactivated by 95.3%. The test solution was applied to Cressia Co., Ltd. Product name: Kimwipe and dried, but it was colorless and odorless.

(Comparative Example 3)
0.08 g of tea extract (Shirai Matsushin Pharmaceutical Co., Ltd., catechin 60W) was dissolved in PBS-T to make a 10% solution. This tea extract solution was carried on a 1 cm square polyester non-woven fabric (weight per unit: 60 g / m 2) corresponding to 0.3 mL to prepare a filter having antiallergenic properties. This filter is immersed in 1 mL of PBS-T containing 50 ng / mL of purified mite antigen (Der f2) (manufactured by Asahi Food and Healthcare Co., Ltd.) and allowed to stand at room temperature for 24 hours. Measured. As a result, the Derf2 allergen was inactivated by 72.0%. However, the test solution was applied to Crecia Co., Ltd., trade name: Kimwipe Kimwipe, and as a result, it turned brown and had a tea-specific odor.

According to the present invention, there can be provided an effective allergen inactivating agent without odor and coloring, and a building component such as a floor and a wall surface, an air conditioner, a textile product such as a woven fabric and a non-woven fabric, a filtering member such as a mask and a filter. It can be widely used even in fields where odors and coloring are problems, and contributes to the industry.

Claims (5)

An aqueous allergen inactivating agent comprising a water-soluble zinc compound. An allergen inactivating agent, wherein a composite particle of a water-insoluble zinc compound and / or water-insoluble zinc / metal oxide is dispersed in water. 3. The allergen inactivating agent according to claim 2, wherein the particle diameter is 50 μm or less. The allergen inactivating agent according to claim 2 or 3, wherein the metal oxide contains at least one of titania, silica, and alumina. The filter which has the antiallergenic property which carry | supports the allergen inactivation agent in any one of Claims 1-4.