JP2006191834A - Lid body of container for biochemicals - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide the lid body of a container for biochemicals, which has excellent organic solvent resistance and with which observation and assay of high accuracy are carried out while covering the container. <P>SOLUTION: The lid body 3 of the biochemical container covering the top of the biochemical container 1 equipped with wells 2. The lid body 3 is constituted of a glass plate 4 covering the tops of the wells 2 and a frame body 5 attached to the circumference of the glass plate 4 so as to reinforce the glass plate 4. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a cover for a biochemical container that covers an upper surface of a biochemical container having a well.

As such a biochemical container, a petri dish that is a dish-shaped glass container with a glass lid is conventionally known. Although the petri dish is made of glass, both the container and the lid are made of glass that is extremely thick and has a low flatness. Therefore, the petri dish has lower light transmittance and has autofluorescence. For example, after culturing cells in a petri dish, it is unsuitable for observing or measuring the cells in the petri dish as they are.
Thus, a cell culture container has been proposed in which at least the bottom of the biochemical container is made of a material having a high light transmittance and a low autofluorescence intensity so that cells cultured in the container can be observed or measured as they are (for example, , See Patent Document 1).
However, the lid of this kind of biochemical container is still conventional, and a lid made of a resin plate or a resin film is used (actually used). However, there are no appropriate patent publications).

Japanese Patent Laid-Open No. 10-295362

Recently, a gene analysis method for observing the hybridization behavior of DNA by UV spectroscopic measurement in a specific environment of reverse micelles has been proposed and attracted attention. In this gene analysis method, a sample is separated from an organic solvent such as isooctane. Become.
Therefore, the resin lid may be dissolved by an organic solvent, which not only affects the measurement accuracy, but is also vulnerable to heat. For example, there is a disadvantage that heat sterilization cannot be performed with the lid on. is there.
In addition, the plastic lid has low light transmittance and high autofluorescence intensity, so it is necessary to remove the lid during observation and measurement, and there is a possibility that fine dust and other objects may get in between. In the case where a volatile component is contained in the culture solution, there is also a drawback that the concentration of the measurement component in the culture solution is changed by volatilization of the volatile component.

  The present invention pays attention to such various conventional problems, and its purpose is excellent in resistance to organic solvents, and enables accurate observation and measurement with the biochemical container kept covered. It is to provide a lid for a biochemical container.

  A first characteristic configuration of the present invention is a lid for a biochemical container that covers an upper surface of a biochemical container having a well, and the lid reinforces the plate glass that covers the upper surface of the well. In order to do so, it is in the place comprised by the frame attached around the plate glass.

According to the first characteristic configuration of the present invention, the lid that covers the upper surface of the biochemical container provided with the well includes a plate glass that covers the upper surface of the well, and a frame that is attached around the plate glass to reinforce the plate glass. In addition to improving the organic solvent resistance by the use of plate glass, if the frame is made of a heat-resistant material, it can be heat sterilized with the lid on it, Due to the reinforcing effect of the body, the plate glass covering the well upper surface can be made as thin as possible.
Therefore, it is possible to increase the light transmittance of the plate glass and suppress the self-fluorescence intensity, and it is not necessary to remove the lid during observation or measurement. Even if a volatile component is contained in the culture solution, volatilization of the volatile component can be suppressed.

  The 2nd characteristic structure of this invention exists in the place where the said plate glass is comprised with 1 sheet glass.

  According to the second characteristic configuration of the present invention, since the plate glass covering the upper surface of the well is formed of a single plate glass, that is, even if the biochemical container includes a large number of wells, all the wells are provided. Since one plate glass covers the upper surface of the cover, the structure of the lid itself is simplified, and the cost can be reduced.

  A third characteristic configuration of the present invention is that, in the one sheet glass, the thickness of the portion corresponding to the upper surface of the well is configured to be thinner than the thickness of the other portion.

  According to the third characteristic configuration of the present invention, in one sheet glass covering the upper surface of the well, the thickness of the portion corresponding to the upper surface of the well is configured to be thinner than the thickness of the other portion. The thin plate thickness portion corresponding to the upper surface of the well is reinforced by the thick plate thickness portion in the other portion. Therefore, the plate thickness of the portion corresponding to the upper surface of the well can be further reduced to further increase the light transmittance.

  According to a fourth characteristic configuration of the present invention, when the upper surface of the well is covered with the lid body, a sealing material for sealing between the lid body and the upper surface of the biochemical container is provided on the lid body. It is in.

  According to the fourth characteristic configuration of the present invention, when the well upper surface is covered with the lid, the lid is provided with the sealing material for sealing between the lid and the biochemical container upper surface. Intrusion of fine dust and other objects into the inside can be prevented more reliably, and even if volatile components are contained in the culture solution, the volatilization of the volatile components can be suppressed more reliably. Can do.

  According to a fifth characteristic configuration of the present invention, when the upper surface of the well is covered with the lid body, a locking portion for locking the lid body to the biochemical container is provided on the lid body. It is in.

  According to the fifth characteristic configuration of the present invention, when the upper surface of the well is covered with the lid, the lid is provided with the locking portion for locking the lid to the biochemical container. In addition to preventing inadvertent dropout of the body, for example, when measuring by vibrating a biochemical container or when using it for vibrating culture where the biochemical container is vibrated, the effect Also become prominent.

  A sixth characteristic configuration of the present invention is that the plate glass is provided with an antireflection layer on at least the upper surface thereof.

  According to the sixth characteristic configuration of the present invention, since the plate glass covering the upper surface of the well is provided with at least the antireflection layer on the upper surface, the light transmittance of the plate glass is further improved, and measurement accuracy by ultraviolet rays or visible light is improved. Can be improved.

  The 7th characteristic structure of this invention exists in the place by which the lower surface of the said plate glass is comprised by the anti-fogging surface.

  According to the seventh characteristic configuration of the present invention, since the lower surface of the plate glass covering the upper surface of the well is configured as an anti-fogging surface, the lower surface of the plate glass is hardly fogged, so that observation of cells and the like in the container is not hindered. It can be carried out.

  The 8th characteristic structure of this invention exists in the place by which the lower surface of the said plate glass is comprised by the hydrophilic surface.

  According to the eighth characteristic configuration of the present invention, since the lower surface of the plate glass covering the upper surface of the well is configured as a hydrophilic surface, even if volatile components contained in the culture solution are condensed on the lower surface of the plate glass. Further, since it spreads thinly over the entire surface of the lower surface of the glass sheet, in addition to the antifogging effect, it is also possible to expect the effect of removing deposits on the lower surface of the glass sheet.

  A ninth characteristic configuration of the present invention is that the lower surface of the plate glass is formed as a water repellent surface.

  According to the ninth characteristic configuration of the present invention, since the lower surface of the plate glass covering the upper surface of the well is configured as a water-repellent surface, when volatile components contained in the culture solution are condensed on the lower surface of the plate glass, While it interferes with the observation of cells in the container, dewed volatile components fall into the culture solution in a short time, so the change in the composition of the culture solution is suppressed and the desired culture is possible. Become.

An embodiment of a biochemical container lid according to the present invention will be described with reference to the drawings.
As a biochemical container, for example, there is a microplate, the microplate 1 has a rectangular shape in plan view, as shown in FIG. 1 and FIG. A number of wells 2 are provided on the upper surface.
The lid 3 used for the microplate 1 is attached to the periphery of the plate glass 4 in order to reinforce the plate glass 4 and a single plate glass 4 covering the upper surfaces of many wells 2 as shown in an exploded view in FIG. It is comprised by the frame 5 made.

The plate glass 4 constituting the lid 3 is formed of various glasses such as quartz glass, high-purity soda lime glass, and high-purity borosilicate glass, and the plate thickness is preferably 0.5 to 0.2 mm. Is set to about.
The plate glass 4 is excellent in light transmittance, and has, for example, a characteristic that a light transmittance of 260 nm is 80% or more and a light transmittance of 400 to 700 nm is 91% or more.
Further, at least the upper surface of the plate glass 4 is subjected to etching, anti-reflective coating, and the like, whereby at least the upper surface is provided with an antireflection layer 8, and if necessary, the upper and lower surfaces are provided with an antireflection layer. 8 is provided.
Further, the lower surface of the plate glass 4 is configured as an anti-fogging surface that is not easily fogged, configured as a hydrophilic surface with hydrophilicity, or configured as a water-repellent surface with water repellency, as necessary. . For example, in order to form a surface sharing antifogging properties and hydrophilicity, a polymer of a hydrophilic polymer and silicon alkoxide is impregnated on the surface of the plate glass 4 or fine irregularities are formed on the surface of the plate glass 4. It becomes possible by performing an appropriate surface treatment, and it is possible to form an anti-fogging surface having only anti-fogging property by forming a film having water absorption on the surface of the plate glass 4.

A frame 5 constituting the lid 3 is composed of a frame-like lower frame 6 and an upper frame 7, and a hanging portion 6 a that hangs downward is integrally provided on the outer peripheral edge of the lower frame 6. Is provided with a step portion 6b for mounting and accommodating the plate glass 4 thereon. The hanging portion 6 a of the lower frame 6 functions as a locking portion for locking the lid 3 to the microplate 1, and is configured to be fitted on the upper surface of the microplate 1.
A drooping portion 7 a that hangs downward is also integrally provided on the outer peripheral edge of the upper frame 7, and the drooping portion 7 a of the upper frame 7 is configured to be externally fitted to the drooping portion 6 a of the lower frame 6.
These upper and lower frames 6 and 7 can be formed of various materials such as resin, metal, ceramics, and wood, but are easy to process and produce, have appropriate strength and elasticity, and easily provide dimensional accuracy. Therefore, vacuum molding with PET is preferable.

The plate glass 4 is placed in the step 6 b of the lower frame 6, the upper frame 7 is covered from above, and the drooping portion 7 a is externally fitted to the drooping portion 6 a of the lower frame 6. The lid 3 is constituted by the frame 5 and the plate glass 4 which are firmly sandwiched by the upper frame 7 and the upper and lower frames 6 and 7.
Further, by covering the upper surface portion of the microplate 1 with the lid body 3, the hanging portion 6 a of the lower frame 6 is firmly fitted on the upper surface portion of the microplate 1, and the lid body 3 is locked to the microplate 1. It is done.

[Another embodiment]
Next, another embodiment will be described. However, in order to avoid redundant description, the components described in the previous embodiment and the components having the same action are denoted by the same reference numerals, and the description thereof is omitted. A configuration different from the embodiment will be described.

(1) In the previous embodiment, the configuration in which the lid 3 is provided with the locking portion 6a for the microplate 1 is shown, but the locking portion 6a is not necessarily indispensable.
For example, as shown in FIG. 4, the lid 3 is constituted by the frame 5 and the plate glass 4 that can be placed on the upper surface of the microplate 1, and the lid 3 is placed on the upper surface of the microplate 1. It can also be configured to cover the upper surfaces of a large number of wells 2.
Further, as shown in FIG. 5, when the sealing material 9 made of silicon rubber or the like is provided on the lower surface of the frame body 5 and the upper surface of the well 2 of the microplate 1 is covered with the lid body 3, It can also be configured to seal between the upper surface of the microplate 1.
Although not shown, the same sealing material 9 can also be provided on the lid 3 in the embodiment shown in FIGS.

(2) In the embodiments described so far, as the plate glass 4 constituting the lid 3, a single plate glass having substantially the same plate thickness over the entire surface is illustrated, but as shown in FIG. It can also be configured and implemented so that the plate thickness t1 of the corresponding portion is thinner than the plate thickness t2 of the other portion.
Such a plate glass 4 can thin a portion corresponding to the upper surface of the well 2 by etching. For example, by etching a 0.5 mm plate glass, t1 is 0.2 mm and t2 is 0.5 mm. 4 can be configured.

(3) In the embodiments described so far, the configuration in which the frame body 5 is disposed only around the plate glass 4 is illustrated. However, as illustrated in FIG. 7, the portion corresponding to the well 2 has a shape corresponding to the well 2. The frame 5 can also be constituted by the lower frame 6 and the upper frame 7 having the openings 6c and 7c.
In another embodiment shown in FIG. 7, as in the previous embodiments, the plate glass 4 is made of one plate glass, but it can be made of two or more plate glasses. As shown in FIG. 8, the same number of plate glasses as the number of wells 2 can be used.
Furthermore, in the embodiment shown in FIGS. 1 to 3, as shown in FIGS. 9 and 10, an annular protrusion 4 a is integrally projected from the lower surface of the plate glass 4 so as to correspond to each well 2, When the lid 3 is externally fitted to the upper surface of the microplate 1, each projection 4 a abuts against the upper surface of the microplate 1 to prevent communication between adjacent wells 2. Such a protrusion 4a can also be provided in the embodiment shown in FIGS.

Cross section of biochemical container and its lid Perspective view of biochemical container and its lid Exploded perspective view of biochemical container lid Sectional drawing of the container for biochemistry and its cover by another embodiment Sectional drawing of the container for biochemistry and its cover by another embodiment Sectional drawing of the container for biochemistry and its cover by another embodiment The partially cutaway perspective view of the lid of the biochemical container according to another embodiment The partially cutaway perspective view of the lid of the biochemical container according to another embodiment Sectional drawing of the container for biochemistry and its cover by another embodiment The perspective view of the cover body of the container for biochemistry by another embodiment

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Biochemical container 2 Well 3 Lid 4 Plate glass 5 Frame 6a Locking part 8 Antireflection layer 9 Sealing material t1 Thickness of part corresponding to well upper surface t2 Thickness of other part

Claims (9)

A lid for a biochemical container covering the top surface of a biochemical container with a well,
A lid for a biochemical container, the lid comprising a plate glass covering the upper surface of the well and a frame attached around the plate glass to reinforce the plate glass.   The lid for a biochemical container according to claim 1, wherein the plate glass is composed of a single plate glass.   3. The cover of a biochemical container according to claim 2, wherein, in the one sheet glass, a plate thickness of a portion corresponding to the upper surface of the well is configured to be thinner than a plate thickness of other portions.   The sealing material which seals between the lid body and the biochemical container upper surface when the top surface of the well is covered with the lid body is provided in any one of claims 1 to 3. The lid of the biochemical container described.   The lid according to any one of claims 1 to 4, wherein a locking portion for locking the lid to the biochemical container when the top surface of the well is covered with the lid is provided on the lid. The lid of the biochemical container described.   The lid for a biochemical container according to any one of claims 1 to 5, wherein the plate glass includes an antireflection layer on at least an upper surface thereof.   The lid of a biochemical container according to any one of claims 1 to 6, wherein a lower surface of the plate glass is configured as an anti-fogging surface.   The lid of a biochemical container according to any one of claims 1 to 6, wherein the lower surface of the plate glass is configured as a hydrophilic surface.   The lid of a biochemical container according to any one of claims 1 to 6, wherein the lower surface of the plate glass is configured as a water repellent surface.

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