JP2006189578A - Transmission illumination system of microscope, and microscope using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To enable transmission illumination observation to be performed by transmission illumination of a sample, even if a focusing handle is arranged near a place that crosses the observing optical axis. <P>SOLUTION: A transmission illumination system 10 is detachably installed, between a stage 11 on which the sample is laid and a focusing pedestal 8 for moving the stage in the observing optical axis direction. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a transmission illumination device for a microscope that is detachably provided to a microscope having an epi-illumination device, for example, and illuminates a specimen, and a microscope using the same.

  For example, Patent Document 1 discloses that a transmission illumination projector is added as a retrofit unit to a microscope body provided with an epi-illumination device. This transmitted illumination projector has a light projecting unit for projecting transmitted illumination light. Further, the microscope main body includes an irradiation unit that irradiates the specimen with transmitted illumination light projected by the transmitted illumination projector. The light projecting unit and the irradiation unit are configured in separate units.

  The light projecting unit is detachably provided on the base side surface of the microscope body. The light projecting unit includes an adapter frame for attaching a light guide for guiding transmitted illumination light, and each optical element as a transmitted illumination optical system for introducing the transmitted illumination light guided by the light guide attached to the adapter frame. (First lens group) and a folding mirror that changes the direction of transmitted illumination light introduced from these optical elements and folds on the observation optical axis on which the objective lens is arranged.

  The irradiation unit is detachably provided between a stage on which the sample is placed and a focusing table for moving the stage up and down. This irradiating unit incorporates each optical element (second lens group) for irradiating the specimen with transmitted illumination light.

With such a microscope, the transmitted illumination light emitted from the light source is guided to the insertion portion by the light guide and introduced into the light projecting portion by the first lens group. The introduced transmitted illumination light is redirected on the observation optical axis by the bending mirror, passes through the second lens group, and is irradiated onto the specimen.
JP 2001-33706 A

  Since the focusing handle of the microscope is operated when the stage is retracted when the sample is focused and the sample is exchanged, its operation frequency is relatively high. Therefore, it is necessary to provide the focusing handle at an optimal position so that the operator can operate it comfortably. For example, the position of the focusing handle is determined by comprehensively considering the physique of the operator who will perform the operation, the size of the microscope body, the eye point position, the position of the operation handle of the stage, and the like. Thereby, the focusing handle may be arranged in the vicinity of crossing the observation optical axis.

  In the transmitted illumination projector disclosed in Patent Document 1, an adapter frame for attaching a light guide to the base side surface of the microscope body is provided to detachably attach the light projecting unit to the base side surface of the microscope body. ing. For this reason, a space for accommodating the light projecting unit is required near the side surface of the base of the microscope main body and the observation optical axis inside the base.

  However, when the focusing handle has to be arranged in the vicinity of the crossing with the observation optical axis from the viewpoint of operation, the focusing handle and its transmission mechanism section occupy the space for housing the above-described light projecting section. For this reason, the above-mentioned transmitted illumination floodlighting device cannot cope with transmitted illumination.

  The present invention is a transmission illumination device for a microscope that is detachably provided between a stage on which a sample is placed and a focusing stage that moves the stage in the direction of the observation optical axis, and irradiates the sample with transmitted illumination light. .

  The present invention relates to a microscope having a stage on which a specimen is placed and a focusing stage that moves the stage in the direction of the observation optical axis. The microscope is detachably provided between the stage and the focusing stage and transmits the specimen. A transmission illumination device that irradiates illumination light, and an intermediate member that is detachably provided between the stage and the focusing table. The intermediate member is a microscope that is provided between the stage and the focusing table when transmission illumination observation is not performed on the specimen and the height position of the stage is varied.

  The present invention can provide a transmission illumination device for a microscope and a microscope using the same, which can transmit and illuminate a specimen to perform transmission illumination observation even when a focusing handle is disposed in the vicinity of the crossing with the observation optical axis.

  Hereinafter, a first embodiment of the present invention will be described with reference to the drawings.

  FIG. 1 shows a configuration diagram of a microscope. A focusing handle shaft 3 is provided on the body frame 2 of the microscope body 1. Focusing handles 4 are provided on the left and right sides of the focusing handle shaft 3 as shown in a partial front view of FIG. The focusing handle shaft 3 is held so as to be able to rotate and slide within a frame 5 fixed to the base portion.

A pinion 6 is formed on the outer periphery of the focusing handle shaft 3, and a rack 7 is engaged with the pinion 6. The rack 7 is attached to the focusing table 8. The focusing table 8 is movably held in the observation optical axis L ₁ direction by a guide 9 which is fixed to the main body frame 2. On the upper surface of the focusing table 8, a transmitted illumination device 10 to be operated later is attached. A stage 11 is assembled on the upper part of the transmission illumination device 10.

Accordingly, when the focusing handle 4 is operated, the focusing handle shaft 3 rotates within the frame 5. Since the rotation of the pinion 6 in conjunction with the rotation of the focusing handle shaft 3 is transmitted to the rack 7, this rack 7 is given if acting on the observation optical axis L ₁ direction. Thus, Asejundai 8 is moved to the observation optical axis L ₁ direction along the guide 9. At the same time, the transmission illumination device 10 and the stage 11 assembled to the focusing table 8 move in the direction of the observation optical axis L ₁ together with the focusing table 8.

An epi-illumination projector 12 is mounted on the upper side of the microscope body 1. A light source 13 for epi-illumination is attached to the back side of the epi-illumination projector 12. The light source 13 emits epi-illumination light 14. An optical member 15 and a half mirror 16 are provided on the optical axis L ₂ of the epi-illumination light 14 emitted from the light source 13. Reflection light path to below the half mirror 16 is coincident with the observation optical axis L _1.

A revolver 17 is rotatably provided on the lower surface of the epi-illumination projector 12. A plurality of objective lenses 18 are attached to the revolver 17. The revolver 17 is arranged a magnification of the objective lens 18 according to the observation of the specimen 19 in the observation light axis L ₁ of the objective lens 18 by rotating.

In the observation light axis L ₁ at the top surface of the vertical illuminator unit 12, the barrel portion 20 is provided. An eyepiece lens 21 is attached to the lens barrel portion 20.

Accordingly, the reflected illumination light 14 from the light source 13 is emitted, the reflected illumination light 14 travels along the optical axis L _2, it passes through the optical member 15, redirected to the lower side by the half mirror 16, traveling along the observation light axis L _1. The epi-illumination light 14 whose direction is changed downward is applied to the specimen 19 through the revolver 17 and the objective lens 18. The observation image 22 from the specimen 19 passes through the objective lens 18, the revolver 17, and the half mirror 16 again and is guided to the lens barrel portion 20, and is enlarged by the eyepiece lens 21.

  A light source device 22 is provided outside the microscope body 1. The light source device 22 is provided with a light source 23 for transmitted illumination and a light control knob 25 for adjusting the brightness of the transmitted illumination light 24. A light guide 26 for transmitting a light beam emitted from the light source 23 is attached to the light source device 22.

FIG. 3 is a partial cross-sectional view of the transmission illumination device 10. The transmitted illumination device 10 includes a base body 27 that is detachable between the stage 11 and the focusing table 8. The base body 27 is formed, for example, the height T _1. The base body 27 is provided with cylindrical holes 28 and 29 that are orthogonal to each other. One cylindrical hole 28 is provided from the side surface of the base main body 27 toward the central portion, and the other cylindrical hole 29 is provided from the central portion of the base main body 27 toward the upper surface. One of the cylindrical bore 28 is coincident with the optical axis _{L 3.} Position of the other of the cylindrical bore 29, when mounted between the quasi-table 8 focus the transmitted illumination device 10 and the stage 11, coincides with the observation optical axis L _1.

  An opening hole 30 is provided on the side surface of the base body 27. The opening hole 30 communicates with one cylindrical hole 28 and has a diameter larger than the diameter of the cylindrical hole 28. A mounting step 31 is provided on the inner wall of the opening hole 30. A light guide mounting portion 32 is fixed to the mounting step 31.

  The light guide attachment portion 32 attaches the light guide 26 to the side surface of the base body 27. An attachment hole 33 and an optical path hole 34 are provided in the light guide attachment portion 32 so as to communicate with each other. An end face 35 is provided in the attachment hole 33. An emission end 26 a of the light guide 26 is inserted into the mounting hole 33. An end surface 26 b of the light emitting end 26 a of the light guide 26 is positioned in contact with the end surface 35 in the mounting hole 33.

  A knob 36 is screwed into the side surface of the light guide mounting portion 32 as shown in FIG. The knob 36 is screwed into the side surface of the light guide mounting portion 32 so that the end surface 36 a of the knob 36 contacts the side surface 26 c of the light guide 26. Accordingly, when the knob 36 is screwed in, the light guide 26 is fixed to the light guide mounting portion 32, and when the knob 36 is loosened, the light guide 26 can be detached from the light guide mounting portion 32.

In the opening hole 30, a light projection lens 37 as an optical element is provided. The light projecting lens 37 is provided in front of the end face 26 b of the emission end 26 a of the light guide 26. The light projecting lens 37 projects the light beam emitted from the end face 26 b of the light emitting end 26 a of the light guide 26 into the opening hole 30. The end face 26b of the light guide 26 corresponds to a light beam emission source. Emitting end 26a and projection lens 37 of the light guide 26 is provided on the optical axis _{L 3.}

On intersection of the observation optical axis L ₁ and the optical axis L ₃ in the base body 27, a mirror 38 is provided. The intersection of the observation optical axis L ₁ and the optical axis L ₃ is also where the one cylindrical hole 28 and the other cylindrical hole 29 intersect. The mirror 38 changes the direction along the light beam projected by the light projecting lens 37 to the observation optical axis L _1.

The other cylindrical hole 29 is provided with an opening hole 39. The opening hole 39 is provided with an irradiation part 40 formed of a cylindrical part protruding from the upper surface 27 a of the base body 27. A condensing lens 41 is provided in the irradiation unit 40. The condensing lens 41 condenses the light beam whose direction is changed by the mirror 38 on the specimen 19 as transmitted illumination light. The stage 11 is provided with an opening hole 42. The opening hole 42 is coaxial to the observation optical axis L _1. Accordingly, the transmitted illumination light transmits and illuminates the specimen 19 through the opening hole 42.

  Next, transmission illumination when the transmission illumination device 10 is attached to the microscope will be described.

  When a light beam is emitted from the light source 23 of the light source device 22, the light beam is guided into the light guide mounting portion 32 through the light guide 26. The light beam guided into the light guide mounting portion 32 is emitted from the emission end 26 a of the light guide 26.

The emitted light beam enters the projection lens 37 from the optical path hole 34 through the opening hole 30. The light beam the incident on the projection lens 37 is projected at the intersection of the observation optical axis L ₁ and the optical axis L ₃ by the projection lens 37, the observation optical axis L ₁ by a mirror 38 provided on the intersection point The direction can be changed upward along the top.

  The light beam whose direction is changed upward is condensed on the specimen 19 by the condenser lens 41 as transmitted illumination light. The brightness of the transmitted illumination light applied to the specimen 19 is adjusted by operating the dimming knob 25 of the light source device 22.

  Next, the attachment / detachment structure of the transmissive illumination device 10 will be described.

  FIG. 5 shows an exploded configuration diagram of the focusing table 8, the transmission illumination device 10 and the stage 11, and FIG. 6 shows a top view of the focusing table 8. On the upper surface of the focusing table 8, convex mounting surfaces 50 and 51 are provided on both sides. Thereby, a recess 52 is formed between the mounting surfaces 50 and 51. However, the wall surfaces 50a and 51a are provided at the boundaries between the mounting surfaces 50 and 51 and the recess 52, respectively. A wall surface 53 is provided between the mounting surfaces 50 and 51 and on the side where the light guide mounting portion 32 is mounted. The mounting surfaces 50 and 51 are provided with screw holes 54 and 55, respectively.

  FIG. 7 shows a top view of the stage 11. The stage 11 is provided with counterbore holes 56. Each bolt 57 is inserted into each of the counterbore holes 56. The lower surface of the stage 11 is a flat mounting surface 58.

  FIG. 8 shows a top view of the transmission illumination device 10. A counterbore 59 and a screw hole 60 are provided in the mounting surface 27 a on the upper surface of the transmissive illumination device 10. The counterbore 59 penetrates the base 27. Bolts 61 are inserted into the counterbored holes 59.

  An attachment surface 27 b is provided on the lower surface of the transmission illumination device 10. The mounting surface 27b is provided with a convex portion 62 protruding downward. This convex part 62 has each wall surface 62a, 62b, 62c, as shown in FIG.

  Next, the attaching / detaching operation of the transmission illumination device 10 to / from the microscope will be described.

  When the transmitted illumination device 10 and the stage 11 are attached, as shown in FIG. 5, the transmitted illumination device 10 is provided on the focusing table 8, and the stage 11 is provided on the transmitted illumination device 10. When the transmissive illumination device 10 is provided on the focusing table 8, the convex part 62 provided on the mounting surface 27 b on the lower surface of the transmissive illumination device 10 is fitted into each wall surface 50 a, 51 a, 53 on the focusing table 8. . At this time, the wall surface 62c of the convex portion 62 contacts the wall surface 53 on the focusing table 8, and the wall surface 62a of the convex portion 62 contacts the wall surface 50a of the focusing table 8, so that the wall surface 62b of the convex portion 62 Comes into contact with the wall surface 51 a on the focusing table 8. Thereby, the mounting position of the transmissive illumination device 10 on the focusing table 8 is determined.

  In this state, the bolt 61 is inserted into a counterbore 59 that penetrates the base 27 of the transmission illumination device 10. The bolt 61 passes through the base 27 of the transmission illumination device 10 and is screwed into the screw hole 54 of the focusing table 8. Thereby, the transmitted illumination device 10 is fixed on the focusing table 8.

  When the stage 11 is provided on the transmitted illumination device 10, the stage 11 applies the lower mounting surface 58 to the upper mounting surface 27 a of the transmitted illumination device 10. At this time, the irradiation unit 40 of the transmission illumination device 10 is inserted into the opening hole 42 of the stage 11. In this state, the bolt 57 is inserted into the counterbore hole 56 that penetrates the stage 11. The bolt 57 passes through the stage 11 and is screwed into the screw hole 68 of the transmission illumination device 10. Thereby, the stage 11 is fixed on the transmission illumination device 10.

  When removing the transmitted illumination device 10 and the stage 11, first, the stage 11 is removed from the transmitted illumination device 10 by loosening the bolts 57. Next, the transmitted illumination device 10 is removed from the focusing table 8 by loosening the bolt 61.

  Next, a configuration for changing the height position of the stage 11 will be described.

As shown in FIG. 9, the microscope includes an intermediate member 70. The intermediate member 70 is provided between the focusing table 8 and the stage 11. The intermediate member 70 is formed, for example, to the height T _2. The intermediate member 70 does not perform transmission illumination observation on the specimen 19 and varies the height position of the stage 11 during epi-illumination observation.

  Mounting surfaces 71 and 72 are provided on the upper surface and the lower surface of the intermediate member 70, respectively. On the attachment surface 72 on the lower surface of the intermediate member 70, a convex portion 73 protruding downward is provided. The intermediate member 70 is provided with a screw hole 74 and a counterbore hole 75. Bolts 76 are inserted into the counterbored holes 75.

  Next, the change in the height position of the stage 11 when performing the epi-illumination observation without performing the transmission illumination observation on the specimen 19 will be described.

  As described above, the transmitted illumination device 10 is removed from between the focusing table 8 and the stage 11 by loosening the bolts 57 and 61.

  When attaching the intermediate member 70 and the stage 11, the intermediate member 70 is provided on the focusing table 8, and the stage 11 is provided on the intermediate member 70. When the intermediate member 70 is provided on the focusing table 8, the convex portion 73 provided on the lower surface of the intermediate member 70 is fitted into each wall surface 50 a, 51 a, 53 on the focusing table 8. Thereby, the mounting position of the intermediate member 70 on the focusing table 8 is determined.

  In this state, the bolt 75 is inserted into the counterbore hole 76 of the intermediate member 70. The bolt 75 passes through the intermediate member 70 and is screwed into the screw hole 54 of the focusing table 8. Thereby, the intermediate member 70 is fixed on the focusing table 8.

  When the stage 11 is provided on the intermediate member 70, the stage 11 applies the lower mounting surface 58 to the upper mounting surface 71 of the intermediate member 70. In this state, the bolt 57 is inserted into the counterbore hole 56 of the stage 11 and screwed into the screw hole 74 of the intermediate member 70. Thereby, the stage 11 is fixed on the intermediate member 70.

As a result, the stage 11 is set higher by the height T ₂ of the intermediate member 70. In this state, epi-illumination observation is performed on the specimen 19 placed on the stage 11.

  Next, the case where the height position of the stage 11 is lowered in the epi-illumination observation will be described.

  The intermediate member 70 and the stage 11 are removed from the focusing table 8 by loosening the bolts 57 and 75.

  Next, the stage 11 is placed directly on the focusing table 8 as shown in FIG. In this state, the bolt 57 is inserted into the counterbore hole 56 of the stage 11, passes through the stage 11, and is screwed into the screw hole 55 of the focusing table 8. Thereby, the stage 11 is fixed on the focusing table 8.

As a result, the height position of the stage 11 is set by a height T ₂ which eliminates the intermediate member 70 low. In this state, epi-illumination observation is performed on the specimen 19 placed on the stage 11.

  As described above, according to the first embodiment, the transmissive illumination device 10 is detachably provided between the stage 11 and the focusing table 8. Thereby, even when transmitting illumination observation is performed, the degree of freedom of the position where the focusing handle 4 is provided can be increased, and the focusing handle 4 can be arranged at an optimal position in terms of operation.

For example, the position of the focusing handle is determined by comprehensively considering the physique of the operator who will perform the operation, the size of the microscope body, the eye point position, the position of the operation handle of the stage, and the like. Thereby, the focusing handle may be arranged in the vicinity of crossing the observation optical axis. Thus even when the focusing handle 4 is provided near the observation optical axis L ₁ and the cross, it can be transmitted illumination observation by irradiating the transmitted illumination light to the specimen 19.

  Since the transmission illumination device 10 is not incorporated in the main body frame 2 of the microscope main body 1, the transmission illumination can be handled at low cost without separately preparing a microscope main body dedicated to transmission illumination.

Light guide mounting portion is disposed 32 to the observation optical axis L ₁ outside and the structure by introducing a light beam emitted from the light guide 26 from the side of the transmissive illumination system 10 deflects along the observation optical axis L1 by the mirror 38 Therefore, the transmission illumination device 10 can be thinned. Thereby, the freedom degree of the setting height of an eye point can be enlarged, and it becomes easy to arrange an eye point in an ergonomic height position.

  Since the intermediate member 70 is detachably provided between the stage 11 and the focusing table 8, if transmission illumination observation is unnecessary, the transmission illumination device 10 is removed and the stage 11 is directly mounted on the focusing table 8. By mounting the stage 11 on the focusing table 8 with the intermediate member 70 interposed, the height position of the stage 11 can be varied. Thereby, even when the transmission illumination observation is unnecessary, the epi-illumination observation can be performed widely from the thin specimen 19 to the thick specimen 19 in a comfortable state while maintaining the eye point height during the transmission illumination observation.

  Since the light beam emitted from the light source device 22 is sent to the transmissive illumination device 10 through the light guide 26, the heat generated in the light source device 22 is difficult to be transmitted to the transmissive illumination device 10, the stage 11 and the focusing table 8, and the performance deteriorates due to heat. It is hard to be affected by.

  Next, a second embodiment of the present invention will be described. The same parts as those in FIG. 3 are denoted by the same reference numerals, and detailed description thereof is omitted.

  FIG. 11 is a partial cross-sectional view of a transmission illumination device in a microscope. This transmitted illumination device 80 has a detachable base body 81 between the stage 11 and the focusing table 8. The attachment / detachment structure of the base body 81 between the stage 11 and the focusing table 8 has the same configuration as that of the transmission illumination device 10 of the first embodiment, and detailed description thereof is omitted.

The base body 81 is provided with a cylindrical hole 82. The cylindrical hole 82 is linearly directed from the side surface of the base main body 81 to the central portion, and is vertically bent upward. An irradiation unit 40 is provided in the opening above the cylindrical hole 82, and a condenser lens 41 is provided in the irradiation unit 40. Therefore, transmission illumination device 80 may provided between the quasi-table 8 focus the stage 11, above the opening of the cylindrical bore 82 is disposed in the observation optical axis L ₁ coaxially.

  A light guide mounting portion 83 is provided at the opening of the cylindrical hole 82 on the side surface of the base body 81. The light guide attachment portion 83 is provided with an attachment hole 84 for attaching the light guide 26. As shown in FIG. 12, a knob 85 for fixing the light guide 26 is provided on the side surface of the light guide mounting portion 83.

A light guide emitting end 86 is provided at the tip of the light guide 26. The light guide emitting end 86 is formed in an L shape by bending the tip portion vertically upward. Emission end face 87 of the light guide 26 is provided to match in the observation light axis L _1.

  Next, transmission illumination when the transmission illumination device 80 is attached to the microscope will be described.

When a light beam is emitted from the light source 23 of the light source device 22, this light beam is guided into the light guide mounting portion 83 through the light guide 26. The light beam guided to the light guide mounting portion 83 is guided by the light guide exit end 86, and is emitted from the exit end face 87 in the observation light axis L _1. The luminous flux emitted from the emission end face 87 is condensed on the specimen 19 as transmitted illumination light by the condenser lens 41.

  Further, similarly to the first embodiment, the intermediate member 70 can be attached and detached between the stage 11 and the focusing table 8. When transmission illumination observation is unnecessary, the transmission illumination device 80 is removed, and the stage 11 is directly mounted on the focusing table 8, or the stage 11 is mounted on the focusing table 8 with the intermediate member 70 interposed therebetween. The height position can be changed. Thereby, epi-illumination observation can be performed in a wide range from a thin specimen 19 to a thick specimen 19 in a comfortable state while maintaining the eye point height during transmission illumination observation.

  As described above, according to the second embodiment, the transmission illumination device 80 including the light guide emitting end 86 formed in the L shape is detachably provided between the stage 11 and the focusing table 8. The same effects as those of the first embodiment can be obtained.

  Next, a third embodiment of the present invention will be described. The same parts as those in FIG. 3 are denoted by the same reference numerals, and detailed description thereof is omitted.

  FIG. 13 shows a partial cross-sectional view of the transmission illumination device in the microscope, and FIG. 14 shows a bottom view of the transmission illumination device. The transmitted illumination device 90 includes a base body 92 that is detachable between the stage 11 and the focusing table 8. A storage space 91 is formed in the base body 92. An electric board 93 is provided in the base body 92. A cover 94 is assembled on the bottom surface side of the base body 92 so as to cover the electric substrate 93.

The electric board 93 is supported in the base main body 92 by the support columns 95 and 96. On the electric substrate 93, a light emitting diode (hereinafter referred to as LED) 97 as an emission source for emitting a light beam is directly mounted. Mounting position of the LED97 is on the observation optical axis _{L 1.}

  On the electrical board 93, an electrical component for lighting the LED 97 is mounted. That is, the input connector 98 is mounted on one side surface of the electric board 93. The input connector 98 is connected to an AC (alternating current) adapter cable 99 (not shown). Thereby, electric power is supplied to LED97 through the cable 99 of an AC adapter. The cable 99 is led out of the base body 92 through a hole 100 provided on the side surface of the base body 92.

  A dimming volume 101 is mounted on the other side surface of the electric substrate 93. The dimming volume 101 performs dimming of the amount of light emitted from the LED 97. The shaft 102 of the dimming volume 101 is led out of the base main body 92 through a hole 103 provided in the cover 94. An operation knob 104 is attached to the shaft 102.

  An irradiation unit 40 is provided in the opening on the upper surface of the base main body 92, and a condenser lens 41 is provided in the irradiation unit 40.

  Next, transmission illumination when the transmission illumination device 90 is attached to the microscope will be described.

When electric power is supplied from the AC adapter cable 99 to the electric board 93 through the input connector 98, the LED 97 mounted on the electric board 93 is turned on. The luminous flux emitted from the LED 97 travels upward on the observation optical axis L ₁ and is condensed on the specimen 19 as transmitted illumination light by the condenser lens 41.

  When the operation knob 104 is operated to rotate the shaft 102 of the dimming volume 101, the amount of light emitted from the LED 97 is dimmed. Thereby, the brightness when the specimen 19 is transmitted and illuminated is adjusted.

  Further, similarly to the first embodiment, the intermediate member 70 can be attached and detached between the stage 11 and the focusing table 8. When transmission illumination observation is unnecessary, the transmission illumination device 90 is removed and the stage 11 is directly attached to the focusing table 8 or the stage 11 is attached to the focusing table 8 with the intermediate member 70 interposed therebetween. The height position can be changed. Thereby, epi-illumination observation can be performed in a wide range from a thin specimen 19 to a thick specimen 19 in a comfortable state while maintaining the eye point height during transmission illumination observation.

  As described above, according to the third embodiment, the transmissive illumination device 90 that houses the electric board 93 on which the LED 97 is mounted is detachably provided between the stage 11 and the focusing table 8. Needless to say, the LED 97 and the electrical component for lighting the LED 97 are mounted on the electric board 93, so that the light source device used in the first and second embodiments is obtained. 22 or the like need not be separately prepared outside the microscope body 1, and the cost can be reduced accordingly.

  Since the LED 97 is provided directly in the base main body 81 of the transmissive illumination device 90, the transmissive illumination device 90 can be made thinner or thinner than the transmissive illumination device 10 of the first embodiment. Thereby, it becomes easy to arrange an eye point at an ergonomic height position.

  The LED 97 is power saving and has a longer life than the halogen lamp, which is widely used for illumination, for example, and is friendly to the environment.

In the third embodiment, one LED 97 is mounted, but a plurality of LEDs 97 may be mounted. The LED 97 is not limited to a type that is directly mounted on the electric substrate 93, and an independently configured type may be used. Instead of causing the light beam emitted from the LED 97 to travel directly on the observation optical axis L ₁ , the light beam emitted from the LED 97 is observed using each optical member such as a mirror and a prism as in the first embodiment. it may be configured to guide on the optical axis L _1.

  Next, a fourth embodiment of the present invention will be described. The same parts as those in FIG. 1 are denoted by the same reference numerals, and detailed description thereof is omitted.

  FIG. 15 shows a configuration diagram of the microscope. An emission end 110 of the light guide 26 attached to the light source device 22 is attached to the introduction portion 111. That is, the introduction part 111 is fitted and fixed in a hole 112 provided on the back surface of the main body frame 2 of the microscope.

  The introduction portion 111 is provided with a first mounting hole 113 on the outer side of the main body frame 2 of the microscope and a second mounting hole 114 on the inner side of the main body frame 2 of the microscope. Yes. The first mounting hole 113 and the second mounting hole 114 communicate with each other through the communication hole 115.

  An emission end 110 of the light guide 26 is inserted into the first mounting hole 113. An end face 113 a is provided in the first mounting hole 113. The end surface 110 a of the light emitting end 110 of the light guide 26 is positioned in contact with the end surface 113 a in the first mounting hole 113.

  A knob 116 is screwed into the outer peripheral surface of the introduction part 111. When the knob 116 is screwed in, the end surface of the knob 116 abuts against the side surface 117 of the emission end 110 of the light guide 26 to fix the light guide 26. The knob 116 makes it possible to remove the light guide 26 from the first mounting hole 113 by loosening.

  An incident end 119 of the relay ride guide 118 is fitted into the second mounting hole 114. An end face 114 a is provided in the second mounting hole 114. The end face of the incident end 119 of the relay ride guide 118 is positioned in contact with the end face 114 a in the second mounting hole 114. Accordingly, the end surface of the incident end 119 of the relay ride guide 118 and the end surface 110a of the output end 110 of the light guide 26 face each other.

The relay ride guide 118 has an incident end 119 on one end side and an emission end 120 on the other end side. The relay ride guide 118 is connected between an incident end 119 and an output end 120 by a flexible snake tube 121. The relay ride guide 118 is disposed in the observation light axis L ₁ of the exit end 120 to avoid the failure portion of such main body frame e.g. epi-illumination provided in the 2 power supply 122 and the focusing handle shaft 3 .

A support member 124 is provided on the inner wall 123 of the main body frame 2. The support member 124 is formed in an L-shape, it is provided in the observation light axis L ₁ of the distal end. A hole 125 is provided at the tip of the support member 124. Further, the distal end of the support member 124, the holding member 126 is erected along the direction of the observation optical axis L _1.

  The holding member 126 is provided with a cylindrical attachment hole 127, and an opening hole 128 is provided at the tip. The mounting hole 127 and the opening hole 128 communicate with each other through the hole 129.

  The attachment hole 127 is provided with an end face 127a. The exit end 120 of the relay ride guide 118 is fitted into the mounting hole 127. The end face of the exit end 120 of the relay ride guide 118 is positioned and fixed in contact with the end face 127a in the mounting hole 127. The end face of the exit end 120 of the relay ride guide 118 serves as an exit source that emits a light beam.

  A lens 130 for projecting a light beam is incorporated in the opening hole 128.

Exit end 120 and the lens 130 of the relay Ride guide 118 is provided coaxially with respect to the observation optical axis _{L 1.}

The Asejundai 8, the observation optical axis L ₁ and the coaxial opening hole 131 is provided. A holding member 126 that holds the emission end 120 of the relay ride guide 118 is inserted into the opening hole 131.

An irradiation member 132 is detachably provided on the focusing table 8. The irradiation member 132 is provided with an illumination light passage hole 133. The position of the illumination light passing hole 133, matching the openings 131, an observation optical axis L ₁ coaxially.

  An annular and convex irradiation unit 134 is provided on the upper surface of the irradiation member 132, and the condenser lens 41 is provided on the irradiation unit 134.

  Next, transmitted illumination in the microscope will be described.

  The stage 11 shown in FIG. 15 is moved upward by an operation on the focusing handle 4 and is in a state where the specimen 19 is in focus.

In this state, the light beam emitted from the light source device 22 is guided to the introducing portion 111 through the light guide 26 and is emitted from the emission end 110 of the light guide 26. The emitted light beam enters the incident end 119 of the relay light guide 118, passes through the relay light guide 118, and is emitted from the output end 120. Since the exit end 120 of the relay light guide 118 is provided coaxially with the observation optical axis L ₁ , the light beam emitted from the exit end 120 of the relay light guide 118 passes through the illumination light passage hole 133. The light travels along the observation optical axis L ₁ and is condensed on the specimen 19 as transmitted illumination light having a predetermined performance obtained by the condenser lens 41. As a result, the specimen 19 is transmitted and illuminated so as to satisfy a predetermined transmitted illumination performance.

  FIG. 16 shows a state where the stage 11 is lowered to the lowest position. In this case, a gap is secured between the bottom surface of the irradiation member 132 and the top surface of the holding member 126 without contact. At the same time, even if the focusing table 8 is lowered to the lowest position, a gap is secured between the lower surface of the focusing table 8 and the upper surface of the support member 124 without contact. Therefore, even if the stage 11 is lowered and retracted to the lowest position, the irradiation member 132 and the holding member 126 do not come into contact with each other, and the focusing table 8 and the support member 124 do not come into contact with each other.

  The brightness of the transmitted illumination light applied to the specimen 19 is adjusted by operating the light control knob 25 of the light source device 22.

  The irradiation member 132 is detachable between the stage 11 and the focusing table 8. Therefore, the height position of the stage 11 is varied by attaching or removing the irradiation member 132 between the stage 11 and the focusing table 8. Thereby, epi-illumination observation can be performed in a wide range from a thin specimen 19 to a thick specimen 19 in a comfortable state while maintaining the eye point height during transmission illumination observation.

As described above, according to the fourth embodiment, the relay ride guide 121 is provided in the main body frame 2 of the microscope main body 1, and the exit end 120 of the relay ride guide 121 is held by the holding member 126 and the support member 124. since there is provided on the observation optical axis L ₁ coaxially, the as in the first embodiment, it is possible to increase the degree of freedom of the position where the quasi handle 4 focus even when performing transmission illumination observation, operational, The focusing handle 4 can be arranged at an optimal position. Thus, focusing handle is sometimes disposed near to cross the observation optical axis, even when provided near the focusing handle 4 to observe the optical axis L ₁ and the cross, transmitted illumination to the specimen 19 Transmitted light observation can be performed by irradiating light.

  Since the irradiation member 132 is detachable between the stage 11 and the focusing table 8, the epi-illumination is widely applied from the thin specimen 19 to the thick specimen 19 in a comfortable state while maintaining the height of the eye point during observation of transmitted illumination. Can be observed.

  Since the introduction portion 111 for introducing the light beam from the light source device 22 is provided on the outer peripheral side of the main body frame 2, a sufficiently wide space can be secured in the vicinity of the focusing handle 4 and the periphery of the operation handle of the stage 11, A comfortable operating environment can be obtained.

  In the fourth embodiment, the lens 130 is provided on the holding member 126 side of the relay light guide 118. However, the lens 130 may be provided in the illumination light passage hole 133 of the irradiation member 132. .

The block diagram which shows 1st Embodiment of the microscope which concerns on this invention. The partial front view of the microscope. The partial cross section figure of the transmission illuminating device used for the microscope. The side view of the transmission illumination apparatus used for the microscope. FIG. 2 is a configuration diagram of a focusing table, a transmission illumination device, and a stage in the microscope. The top view of the focusing stand in the microscope. The top view of the stage in the microscope. The top view of the transmission illumination apparatus in the microscope. The figure which shows the attachment structure of the intermediate member in the microscope. The figure which shows the attachment of the stage on the focusing stand in the microscope. The partial cross section figure of the transmission illumination apparatus in 2nd Embodiment of the microscope which concerns on this invention. The side view of the transmission illumination apparatus used for the microscope. The partial cross section figure of the transmission illuminating device in 3rd Embodiment of the microscope which concerns on this invention. The bottom view of the same transmissive illuminating device. The block diagram which shows 4th Embodiment of the microscope which concerns on this invention. The figure which shows the state which lowered the stage of the microscope to the lowest position.

Explanation of symbols

  1: microscope body, 2: body frame, 3: focusing handle shaft, 4: focusing handle, 5: frame, 6: pinion, 7: rack, 8: focusing table, 9: guide, 10: transmission illumination device , 11: stage, 12: epi-illumination projector, 13: light source, 14: epi-illumination light, 15: optical member, 16: half mirror, 17: revolver, 18: objective lens, 19: specimen, 20: lens barrel , 21: eyepiece lens, 22: light source device, 23: light source, 24: transmitted illumination light, 25: light control knob, 26: light guide, 27: base body, 28, 29: cylindrical hole, 30: aperture hole, 31 : Mounting step, 32: light guide mounting portion, 33: mounting hole, 34: optical path hole, 35: end face, 26a: light guide exit end, 26b: end face of light guide, 36: knob, 36a: end face of knob, 26c: side surface of light guide, 3 : Projection lens, 38: Mirror, 39: Opening hole, 40: Irradiation part, 41: Condensing lens, 42: Opening hole, 50, 51: Mounting surface, 52: Recess, 50a, 51a, 53: Wall surface, 54 55: Screw hole, 56: Counterbore hole, 57: Bolt, 58: Mounting surface, 59: Counterbore hole, 60: Screw hole, 61: Bolt, 62: Projection, 62a, 62b, 62c: Wall surface, 70: Intermediate Member, 71, 72: Mounting surface of intermediate member, 73: Projection, 74: Screw hole, 75: Counterbore hole, 76: Bolt, 80: Transmitting illumination device, 81: Base body, 82: Cylindrical hole, 83: Light Guide mounting portion, 84: mounting hole, 85: knob, 86: light guide exit end, 86: light guide exit end, 87: light guide exit end surface, 90: transmission illumination device, 91: storage space, 92: base body , 93: Electric substrate, 94: Bar, 95, 96: support, 97: light emitting diode (LED), 98: input connector, 99: cable, 100: hole, 101: dimming volume, 102: axis of dimming volume, 103: hole, 104: operation Knob, 110: Light guide exit end, 111: Introducing section, 112: Hole, 113: First mounting hole, 114: Second mounting hole, 115: Communication hole, 113: End face of the first mounting hole, 110a: End surface of the light guide, 113a: End surface of the first mounting hole, 116: Knob, 117: Side surface of the light guide, 118: Relay ride guide, 119: Incident end of the ride guide for relay, 120: Ride for relay Guide exit end, 121: snake tube, 122: epi-illumination power supply, 123: inner wall of the main body frame, 124: support member, 125: hole, 126: holding member, 127: mounting hole, 12 8: Opening hole, 129: Hole, 130: Lens, 131: Opening hole, 132: Irradiation member, 133: Illumination light passage hole, 134: Irradiation part.

Claims (19)

  Transmission of a microscope, which is detachably provided between a stage on which a specimen is placed and a focusing stage that moves the stage in the direction of the observation optical axis, and irradiates the specimen with transmitted illumination light Lighting device. A detachable base body between the stage and the focusing table;
An emission source provided on the base body for emitting a luminous flux;
A light projecting / collecting optical system that is provided in the base body and emits the light beam emitted from the emission source along the observation optical axis to illuminate the sample as transmitted illumination light;
The transmission illumination device for a microscope according to claim 1, wherein: The emission source is provided outside the observation optical axis,
The light projecting / collecting optical system includes an optical element that changes the direction of the light beam emitted from the emission source and guides the light beam to the observation optical axis.
The transmission illumination apparatus for a microscope according to claim 2. The emission source is an emission end of a light guide that guides the light beam output from a light source device provided outside the microscope.
4. The transmission illumination device for a microscope according to claim 2, wherein the transmission illumination device is a microscope. A light guide mounting portion that attaches the light emitting end of the light guide to the base body and makes it removable.
The transmission illumination device for a microscope according to claim 4.   6. The transmission illumination device for a microscope according to claim 5, wherein the light guide mounting portion is provided on a side surface of the base body. The light projecting / collecting optical system includes a light projecting lens that projects the light beam emitted from the emission source,
A mirror that changes the direction of the light beam projected by the light projecting lens and guides it to the observation optical axis;
A condensing lens for condensing the luminous flux whose direction has been changed by the mirror as the transmitted illumination light on the specimen;
The transmission illumination device for a microscope according to claim 2, comprising:   3. The transmission illumination device for a microscope according to claim 2, wherein the emission source is provided on the observation optical axis. The emission source is an emission end of a light guide that guides the light beam output from a light source device provided outside the microscope,
The exit end of the light guide is provided on the observation optical axis,
The transmission illumination apparatus for a microscope according to claim 2.   9. The transmission illumination apparatus for a microscope according to claim 8, wherein the emission source includes a light emitting element that emits the light flux.   11. The transmission illumination device for a microscope according to claim 10, wherein the light emitting element is a light emitting diode.   The transmission illumination device for a microscope according to claim 11, further comprising an electrical unit that is provided in the base body and lights the light emitting diode.   13. The transmission illumination device for a microscope according to claim 12, wherein the electrical unit includes a light control unit that adjusts a light amount of the light beam emitted from the light emitting diode. A stage for placing the specimen;
A focusing stage for moving the stage in the direction of the observation optical axis;
A light guide for relay that is provided in the main body of the microscope and guides a light beam incident from the outside of the main body of the microscope;
A light guide light projecting unit that projects the light beam guided by the relay light guide onto the observation optical axis;
An irradiating member that is detachably provided between the stage and the focusing table, and irradiates the sample with the light beam projected by the light guide light projecting unit as transmitted illumination light,
A transmission illumination device for a microscope, comprising:   15. The transmission illumination apparatus for a microscope according to claim 14, wherein the irradiation member has a condensing optical system that irradiates the specimen with the light beam as the transmission illumination light. The light guide light projecting unit has a support member that supports the inside of the microscope main body so that the axis of the emission end of the relay light guide coincides with the observation optical axis.
The transmission illumination device for a microscope according to claim 14. At the incident end of the light guide for relay, a light guide that guides the light beam from the outside of the microscope is attached, is removable, and an incident end that receives the light beam from the light guide is provided,
The incident end is provided on the microscope body.
The transmission illumination device for a microscope according to claim 14. In a microscope having a stage for placing a specimen and a focusing stage for moving the stage in the direction of the observation optical axis,
The transmitted illumination device according to any one of claims 1 to 15, wherein the transmitted illumination device is detachably provided between the stage and the focusing table, and irradiates the transmitted illumination light to the specimen.
An intermediate member detachably provided between the stage and the focusing table;
Comprising
The transmitted illumination device is provided between the stage and the focusing table when the specimen is observed for transmitted illumination,
The intermediate member is provided between the stage and the focusing table when the transmitted illumination observation is not performed on the sample and the height position of the stage is changed.
A microscope characterized by that. In a microscope having a stage for placing a specimen and a focusing stage for moving the stage in the direction of the observation optical axis,
The transmission illumination device according to any one of claims 14 to 17, comprising:
The intermediate member is changed by attaching and removing between the stage and the focusing table when changing the height position of the stage,
The intermediate member is provided between the stage and the focusing table when the transmitted illumination observation is not performed on the sample and the height position of the stage is changed.
A microscope characterized by

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