JP2006169340A - Information-carrying nucleic acid-containing leather material and its manufacturing process - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an information-carrying nucleic acid-containing leather material which provides unremovable individual authentication information and enables identification of its source, history and the like by decoding the information, a manufacturing process of the information-carrying nucleic acid-containing leather material and a leather product using the leather material. <P>SOLUTION: The leather material utilizes a sequence of nucleic acids as represented by DNA's as an individual authentication information contains information-carrying nucleic acids having an arbitrary and known base sequence preferably in an amount of 0.5-500 μg of 100 g of the leather material. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a leather material and a leather product, and more specifically, an information nucleic acid-containing leather material and leather product containing an information nucleic acid that can be used for individual authentication such as source / history information, and further, the information nucleic acid-containing product The present invention relates to a method for manufacturing a leather material.

For individual authentication so far, watermark printing such as license plates, banknotes, IC chips, credit card photos, etc. are used.
However, these individual authentication means have a drawback that they can be removed from the product by peeling, cutting, erasing or the like. For this reason, the development of authentication information that cannot be removed from the product and is not lost is expected.

On the other hand, DNA is an information biomolecule originally possessed by all living organisms and containing all genetic information in each organism, and many of them correspond to amino acid sequences of many proteins. That is, in DNA, deoxyadenosine (dA), deoxyguanosine (dG), deoxycytosine (dC), and deoxythymine (dT) are bound with a certain direction through a phosphate ester bond, and the number of bases If n is ^n, there are 4 ⁿ types of DNA of that length. Therefore, for example, there can be about 4.3 billion types of DNA that can be distinguished even with only 16 types of bases.
At present, any DNA having several tens of nucleotide sequences can be arbitrarily synthesized, and if there is a certain amount or more, an automatic sequence reader (sequencer) is used. The sequence can be determined automatically.

As a technique for applying such DNA, the use of a forgery-preventing label in which DNA is contained in a water-insoluble medium is used for the product, and the presence or absence of the DNA is used as a clue to clarify the authenticity of the product. Has been proposed (see Patent Document 1).
JP 2004-159502 A

However, the technique described in Patent Document 1 basically relates to a method of mixing DNA and a water-insoluble medium, and Patent Document 1 discloses a ribonucleic acid obtained by PCR as a method for confirming authenticity of a product. It is only suggested to identify the target product containing ribonucleic acid by confirming the presence or absence of amplification.
In addition to authenticity verification data that uses the presence / absence of DNA as a test index, there is no disclosure of data related to individual authentication that enables authentication for each product of the same type of product using the DNA sequence as a test index. Not.

  On the other hand, if we can establish a technology that allows information related to the origin and history to be comprehensively included in leather products such as bags, belts, shoes, and clothing, and that it can be authenticated as necessary, these can be established. It is now possible to authenticate the origin and history individually.For example, identification of victims from clothing and personal belongings at the time of an accident or disaster, identification of the criminal from leftovers at the time of crime, identification of the owner from stolen goods, There is a demand for establishing such technical means, which can be widely used for authenticating brand products.

  The present invention has been made in view of such problems in the prior art, and its object is to provide individual authentication information that cannot be removed, and by deciphering this information, its origin and history It is to provide a leather material containing an information nucleic acid, a method for producing a leather material containing such an information nucleic acid, and a leather product using the leather material.

  Based on the idea of using the sequence of a nucleic acid represented by DNA as individual authentication information as a result of intensive studies to solve the above problems, the present inventors have included the nucleic acid in leather, The inventors have found that the above problems can be solved by reading the base sequence as necessary, and have completed the present invention.

  That is, the present invention is based on the above findings, and the information nucleic acid-containing leather material of the present invention includes an information nucleic acid having a site having an arbitrary and known base sequence in the comparison. In the method for producing an information nucleic acid-containing leather material of the present invention, a solution containing the information nucleic acid, for example, a dyeing solution is allowed to permeate the material leather. The above-mentioned information nucleic acid-containing leather material of the present invention is used.

  According to the present invention, since the information nucleic acid is included in the leather material, the information nucleic acid is extracted from the leather product to be investigated, and the base sequence is determined to determine the product information using the base sequence as a detection index. For example, individual authentication of the origin / history data becomes possible, and an extremely excellent effect is obtained that information on the origin / biography of the subject of the survey can be obtained. In addition, such information nucleic acids cannot be visually recognized and can be distributed over a wide range throughout the leather material, so it is practically impossible to remove them, and excellent individual authentication means It becomes.

  Hereinafter, the information nucleic acid-containing leather material of the present invention and the production method thereof will be described in more detail. In the present specification, “%” indicates a mass percentage unless otherwise specified.

The information nucleic acid-containing leather material of the present invention contains an information nucleic acid having a site having an arbitrary and known base sequence.
In the present invention, “leather” means not only natural leather collected from various animals but also synthetic leather. The leather product refers to a bag, a bag, a wallet, shoes, a belt, clothing, a hat, exercise equipment, furniture, an interior material for construction or automobile, and the like made of the leather material as described above.

Here, the information nucleic acid is provided with a part having an arbitrary and known base sequence, and as described above, by preparing in advance information-enriched nucleic acid having a different base sequence in a product, the base sequence It is possible to perform individual authentication of various product information using, for example, origin / history data, and to be an individual authentication means that is virtually impossible to remove from the product.
And this information nucleic acid refers to DNA (deoxyribonucleic acid), RNA (ribonucleic acid) and derivatives thereof, which may be natural or artificial, but considering that they are added to products with harsh usage environments, It is preferred to use a structurally stable artificial mold. In the artificial type, a sequence having a binding mode that does not exist in the natural type (for example, a linkage between nucleosides includes not only a phosphate ester bond but also a non-natural type such as a thiophosphate ester bond) may be formed. it can.

  In the information nucleic acid, that the base sequence site is arbitrary means that it can be randomly selected as long as it is a detectable base sequence, and the base sequence site is known. This means that the base sequence used for individual authentication is known in advance.

As the size of the information nucleic acid, the number of bases in the whole nucleic acid is preferably 200 or less. That is, when the number of bases exceeds 200, unreacted sites are generated little by little at the stage of synthesis, and the content of what is covered by the base tends to increase. In addition, it is more preferable that it is about 100 bases.
Moreover, it is preferable that thymine does not adjoin in the said base sequence from a viewpoint of suppressing that thymine dimerizes.

Furthermore, the information nucleic acid is preferably derivatized with a protecting group from the viewpoint of improving stability when used in combination with a compound that reacts with an OH group or when used in a severe environment, Specifically, the hydroxyl group at one or both of the 5′-position and the 3′-position is derivatized with a phosphate group, acyl group, alkoxycarbonyl group, benzyl group, substituted benzyl group, allyl group, or the like. be able to.
FIG. 1A shows natural DNA, and FIG. 1B shows DNA derivatized at the 5 ′ position.

  From the viewpoint of enhancing the convenience of isolation and purification, it is preferable to derivatize the hydroxyl group at the 5 ′ position with biotin or a fluorescent molecule. Specifically, when vithion is used, it is easily adsorbed selectively on a column to which a protein called avidin is bound. On the other hand, when a fluorescent molecule such as fluorescein is used, the nucleic acid itself becomes fluorescent, so that it can be detected with high sensitivity and purification and the like are facilitated. Thus, individual authentication becomes very easy by improving the convenience of isolation and purification.

Further, for example, when the 5′-position is substituted with sulfur, it can be easily separated by passing the product extracted with water through a column of a carrier coated with gold (Au).
When RNA is used as the information nucleic acid, the 2′-position hydroxyl group can be derivatized with the above-described protecting group from the viewpoint of improving stability.

  In addition, in the case of performing individual authentication by extracting an information nucleic acid, the base sequence portion may be a portion used for amplification of the information nucleic acid from the viewpoint of efficiently detecting even a small content. preferable. As a method for amplifying such an information nucleic acid, a polymerase chain reaction (PCR) that can be synergistically amplified can be appropriately employed.

In the PCR method, even if the amount of information nucleic acid is extremely small, it can be amplified extremely. For example, while controlling the temperature in the presence of a base (primer) complementary to several tens of bases of DNA, When the sex DNA polymerase is allowed to act, the original DNA can be doubled. For example, if this is repeated 30 times, it can be amplified several hundred million times.
Such amplification makes it possible to obtain a sufficient amount of DNA to determine its sequence even from a very small amount of sample. As a result, the “identity” of the product that contained it from the information corresponding to the sequence is obtained. Will be revealed.

At this time, it is preferable that the sites used for the amplification have primer-corresponding sites necessary for polymerase chain reaction (PCR) at both ends. That is, as the information nucleic acid, a nucleic acid without a primer can be used, but by providing a primer, it becomes possible to identify in a shorter time.
For such a primer corresponding site, the lower limit of the number of bases is preferably 5. More preferably, it is 10 or more. When the number of bases is less than 5, the number of distinguishable nucleic acids decreases, and it takes time to identify many products. On the other hand, the upper limit of the number of bases is preferably 100. This is because when the number of bases exceeds 100, the ratio of by-products lacking a base at any position becomes high, so that purification takes time and tends to be difficult in some cases.
In addition, when RNA is used as the information nucleic acid, DNA complementary to the sequence can be obtained using reverse transcriptase, and PCR can be performed using this DNA.

Further, the information nucleic acid preferably further has an authentication information site in addition to the base sequence site, whereby individual authentication can be performed with more detailed information setting.
For example, as shown in FIG. 2, in the case of information-oriented DNA having primer-corresponding sites at both ends, a sequence with m bases is placed in the center (B ₁ -B _m ), and the sequence information of this part is authenticated. Correspond to information. At both ends, sequences (X _{1 to} X ₁ , P _{1 to} P _n ) complementary to l (el) and n primers, respectively, are linked. The presence of this part makes it possible to adopt the PCR method for the first time.

The information DNA can be used as an information element of this single-stranded DNA or a double-stranded DNA complexed with a complementary sequence of DNA. The sequence of the primer corresponding site can be devised so that the binding of complementary sequences is as stable as possible and amplification by the PCR method proceeds smoothly.
In FIG. 2, X _{1 to} X ₁ , B _{1 to} B _m , and P _{1 to} P _n are deoxyadenosine (dA), deoxyguanosine (dG), deoxycytosine (dC), and deoxythymine (dT), respectively. It is comprised from either.

In the individual authentication of a product using the base sequence of the information nucleic acid as a detection index, when the information nucleic acid is amplified by the PCR method, the extracted information nucleic acid solution, PCR buffer solution, sterile distilled water, at least 1 Mixing seed primer, 2,3-dideoxynucleoside triphosphate (dNTP) and polymerase
(1) Heat at 92-95 ° C for 2-5 minutes, then (2) a: 92-95 ° C for 30-60 seconds,
b: 30 to 60 seconds at 20 to 50 ° C.,
c: 70 to 80 ° C. for 30 to 120 seconds
The heating cycle is repeated 20 to 50 times, and thereafter (3) It is preferable to heat treatment at 70 to 80 ° C. for 1 to 10 minutes.
At this time, it is desirable to use two kinds of primers from the viewpoint of enhancing the arbitraryness of the basic sequence of the information nucleic acid.

Here, regarding the condition range of (1), it becomes difficult to separate DNA into double strands in a short time at a temperature lower than 92 ° C. × 2 minutes, and in a long time at a temperature higher than 95 ° C. × 5 minutes, However, it is particularly preferable to heat at 94 ° C. for 5 minutes. In addition, when the information nucleic acid contained in a leather material is single strand, this (1) process is unnecessary.
In addition, the condition range in (2) -a is that the amplification factor decreases in a short time at a temperature lower than 92 ° C. × 30 seconds, and the enzyme is deactivated in a long time at a temperature higher than 95 ° C. × 60 seconds. It is particularly preferable to heat at 94 ° C. for 30 seconds.
Furthermore, in the condition range of (2) -b, it is difficult to bind the primer and DNA in a short time at a temperature lower than 20 ° C. × 30 seconds, and the enzyme is lost in a long time at a temperature higher than 50 ° C. × 60 seconds. Although it depends, it is particularly preferable to heat at 40 ° C. for 30 seconds.
Further, the condition range of (2) -c is due to the fact that the elongation becomes insufficient in a short time at a temperature lower than 70 ° C. × 30 seconds and the enzyme is deactivated in a long time at a temperature higher than 80 ° C. × 120 seconds. It is particularly preferable to heat at 72 ° C. for 30 seconds. .

And in (3), it is especially preferable to heat at 72 degreeC for 7 minutes. That is, elongation is insufficient in a short time at a temperature lower than 70 ° C. × 1 minute, and time is wasted in a long time at a temperature higher than 80 ° C. × 10 minutes.
Further, regarding the number of repetitions of the heating cycles a to c in (2) above, if the number is less than 20, the amplification factor decreases, and if the number is more than 50, time is wasted. preferable.

FIG. 3 is a process diagram showing an embodiment of the individual authentication procedure of a product using the above-described information nucleic acid. As shown in the figure, first, in S1, information DNA is obtained from a product, that is, a leather material or a leather product. Extract.
Next, in S2, after the extraction solution is concentrated by freeze-drying, in S3, two kinds of primers and a polymerase are added.

Next, in S4, DNA is amplified by repeating PCR, and in S5, the remaining excess primer is decomposed by a single-stranded DNA cleavage enzyme.
In S6, the double-stranded information DNA is purified by gel filtration. In S7, the sequence of the information DNA is determined by a sequencer.

  In the above S1, the leather material may be powdered and mixed with a small amount of water. However, when the information DNA is carried on the fine particles and chemically bound, for example, it is efficiently extracted by hydrolysis. Will be able to. Moreover, in S2, you may concentrate using a centrifugal evaporator, for example. Further, in S5, for example, Taq DNA polymerase, Tth DNA polymerase, Tf1 DNA polymerase, Vent polymerase, Pfu polymerase, Bca BEST polymerase, KOD DNA polymerase or the like can be used as the single-stranded DNA cleaving enzyme. In addition, the target DNA can be amplified between S6 and S7 by repeating the same operations as in S3 and S4. In S7, the sequence may be determined by a mass spectrometer or may be combined with the split determination by a sequencer.

In determining the base sequence, it is desirable to compare information nucleic acid data extracted from a product with an information nucleic acid database including at least the information nucleic acid data. By comparing with a database of informatized nucleic acids ascertained in advance, the time required for product authentication can be greatly reduced.
Examples of data stored in such a database include electrophoresis time and movement distance when gel filtration is performed (this may be achieved by simply flowing the information nucleic acid itself to the control lane).

The content of the information nucleic acid in the information nucleic acid-containing leather material and leather product of the present invention is preferably 0.5 to 500 μg, more preferably in the range of 50 to 100 μg with respect to 100 g of the material leather. Is more preferable.
If the information nucleic acid content is less than 0.5 μg, there is a possibility that the information nucleic acid cannot be detected from the target product, such as leather materials or leather products, and the base sequence cannot be determined. Is costly and wasteful.

In addition, the information nucleic acid is supported in fine particles such as silica, zinc oxide, titanium oxide, molybdenum oxide, tungsten oxide, and barium titanate, rather than being contained in the leather material as it is as a single substance. In this way, the information nucleic acid can be reliably and stably added and dispersed in the leather material, and can be stably maintained in the leather material for a long period of time. This makes it possible to perform individual authentication of the origin and history of a product using such a base sequence of information nucleic acid as a detection index over a long period of time.
Here, “supporting” means that the information nucleic acid is in close contact with the fine particles so that they can move together with the fine particles, and the information nucleic acids are firmly attached to the surface of the fine particles. Even if it has adhered, it may adhere to the surface of the fine particles or its recesses with a strength sufficient to withstand use.

  When the information nucleic acid is supported on the fine particles as described above, the fine particles are dispersed in sterilized distilled water to form a suspension, and the information nucleic acid is left in the suspension as it is or the information nucleic acid is used. An information nucleic acid aqueous solution dissolved in sterilized distilled water can be added and dried. At this time, the information nucleic acid may be added as it is without making it an aqueous solution, and the rest may be added in the state of an aqueous solution.

At this time, the suspension includes alcohol (eg, methanol, ethanol, propanol, butanol, pentanol, hexanol, heptanol, octanol, nonanol, etc.), ester (eg, ethyl acetate, butyl acetate, propyl acetate, etc.) ), Ketones (for example, acetone, dimethyl ketone, methyl ethyl ketone, diethyl ketone, etc.) and aromatic solvents (toluene, hexane, cyclohexane, xylene, etc.) are also desirably added. As well as improving dispersibility, the volatilization of water and solvent after the addition of the information nucleic acid is promoted.
In addition, these solvents are not limited to only 1 type, It is also possible to use 2 or more types of solvents together. These solvents may be added at the same time as the information nucleic acid or after the information nucleic acid is added.

As the amount of the solvent added, in the case of alcohol, the volume ratio of sterilized distilled water / alcohol is preferably in the range of 1 to 99. In the case of a solvent other than alcohol, that is, the ester, ketone, aromatic solvent In some cases, the volume ratio of sterile distilled water / solvent is preferably in the range of 1 to 75.
This is because if the addition amount of the solvent is too small, the above-mentioned effect due to the addition of the solvent cannot be obtained sufficiently, and conversely, even if it is too much, the water tends to remain without volatilization due to a decrease in compatibility with water. This is because there is a tendency to become insufficient.

  The size of the fine particles is desirably in the range of 0.01 to 1 μm, preferably 0.02 to 0.1 μm in average particle size. This is because if the average particle size of the microparticles is less than 0.01 μm, the detection accuracy of the information nucleic acid decreases, and if it exceeds 1 μm, the amount of penetration into the leather decreases, making detection difficult and the microparticles sedimenting. It becomes easy to do.

  The carrying amount of the information nucleic acid in the fine particles is desirably in the range of 0.5 to 10,000 μg, more preferably 1.0 to 1000 μg with respect to 100 g of the fine particles. When the loading amount of the above-mentioned information nucleic acid is less than 0.5 μg per 100 g of fine particles, the information nucleic acid cannot be stably added to the product, and the detection accuracy is lowered. On the other hand, when it exceeds 10,000 μg This is because the information nucleic acid cannot be stably supported on the fine particles, and the free information nucleic acid tends to be generated and difficult to disperse.

  In the information nucleic acid-containing leather material of the present invention, the amount of the fine particles added is preferably 0.5 to 50 g, more preferably 0.5 to 2 g, with respect to 100 g of leather. If the amount of fine particles added is less than 0.5 g per 100 g of leather, the amount of fine particles being sampled is reduced, the detection accuracy of the information nucleic acid is reduced, and if it exceeds 50 g, fine particles are not contained in the staining solution. May settle and condense.

In order to make the leather material contain the information nucleic acid, it can be carried out by infiltrating the material leather with a liquid added with the information nucleic acid, preferably in a state of being supported on fine particles, and then drying. In addition, when dye | staining a leather raw material, it is desirable to use the dyeing | staining liquid which added information nucleic acid, and dyeing | staining and addition of information nucleic acid can be performed simultaneously by this.
In the case of synthetic leather, it is also possible to contain information nucleic acid inside the leather by using a resin raw material to which information nucleic acid has been added in advance.

  Hereinafter, the present invention will be described in more detail based on examples, but the present invention is not limited to these examples.

[1] Preparation of information nucleic acid-carrying fine particles [1-1] Synthesis of information DNA Sequences of TGCACGCACCGTGTACTC and AGTGGACACGTTGGTCGG at both ends of the authentication information site having the sequence of GGGATATATGGAGG by sequentially binding nucleosides by the phosphoramidite method Primer-corresponding sites having γ were bound to each other to synthesize information DNA (5′-TGCACGCACCGTGTACTTC-GGGATATATGGAGG-AGTGGACACGTTGGTCCG-3 ′) as an information nucleic acid.

[1-2] Preparation of Suspension Zinc oxide (ZS-032 manufactured by Showa Denko KK) having an average particle diameter of 0.02 μm and silica having an average particle diameter of 1 μm (Suzuki) as fine particles for carrier 3 types of silica (E-2C manufactured by Yushi Kogyo Co., Ltd.) and silica having an average particle size of 4 μm (E-16C manufactured by Suzuki Yushi Kogyo Co., Ltd.) were used. 15 mL of sterilized distilled water was added and stirred to obtain a suspension of each fine particle.

[1-3] Addition of informational DNA 10 nmol (164 μg) of informational DNA obtained in [1-1] above is dissolved in 15 mL of sterile distilled water, and the obtained aqueous solution of informational DNA is added to each of the above suspensions. Stir well and add 4 mL of ethanol and stir.

[1-4] Natural Drying Each of the suspensions to which the informational DNA had been added was poured into a plastic circular dish, and naturally dried for two days with the top covered with paper or cloth having good air permeability.

[1-5] Drying under reduced pressure What was almost dried by the above [1-4] was put into a desiccator, dried under reduced pressure under heating at 40 ° C., and sufficiently dried.

[1-6] Pulverization According to the above [1-5], each of the finely dried and lump-like fine powders was put in a mortar and crushed to obtain information nucleic acid-carrying fine particles as the original fine powder.

[2] Preparation of informational DNA-containing leather material [2-1] Preparation of staining solution Various informational nucleic acid-carrying fine particles having different particle diameters and carrying amounts of informational DNA prepared as described above are dyed by Pillar Aiton color It added in the ratio of 1.0g with respect to 100g of liquids, and each stirred for 1 hour.

[2-2] Impregnation of information-added DNA-added staining solution Each staining solution prepared as described above was sprayed on 1000 g of 5 mm-thick cowhide by spraying, and then dried in an atmosphere at 80 ° C. for 20 minutes. The leather material shown in Example 1 was obtained. As for Examples 2 to 20, as shown in Table 1, by using the same method as in Example 1 except that the amount of added information DNA, the diameter of fine particles, and the amount added were changed corresponding to Table 1. Twenty kinds of leather materials having different information DNA content, fine particle addition amount, and fine particle diameter were obtained.

[3] Detection of DNA [3-1] Cutting of Leather Piece Each piece of leather in each of the above examples was cut finely with a cutter.

[3-2] DNA extraction 5 mL of sterilized distilled water was added to each of the obtained leather strips, and the mixture was stirred with a magnetic stirrer to extract informational DNA contained therein into an aqueous layer.

[3-3] Concentration of aqueous solution Using a centrifuge, each leather strip and the aqueous layer were separated, and the aqueous layer was concentrated using a centrifugal evaporator.

[3-4] Preparation of treatment solution Each of the DNA solutions (5 μL) eluted and collected was mixed with PCR buffer (5 μL), Taq polymerase (0.25 μL), sterile distilled water (24.75 μL), 5 μM first primer (5 '-TGCACGCACCGTGTACTC-3': 5 μL), 5 μM of the second primer (5′-CCGACCAACGGTCCACT-3 ′: 5 μL), and 2 mM of 2,3-dideoxynucleoside triphosphate (5 μL) were mixed.

[3-5] Thermal cycle After heating each said liquid mixture at 94 degreeC for 5 minutes, the cycle of 30 second heating at 94 degreeC-> 30 second heating at 40 degreeC-> 30 second heating at 72 degreeC was repeated 30 times, respectively.

[3-6] Storage Each of the mixed liquids subjected to the above heating cycle was subjected to a heat treatment at 72 ° C. for 7 minutes, and then stored at 4 ° C.

[3-7] Purification Using a single-strand DNA cleavage enzyme S1 nuclease (reacts specifically with single-strand DNA), the excess primer was decomposed and the target double-strand information DNA was purified by gel filtration. .

[3-8] Addition of primer and dNTP One kind of primer 5′-TGCACGCACCGTGTTACTC-3 ′ and fluorescently labeled 2,3-dideoxynucleoside triphosphate (dNTP) were mixed with the purified information DNA.

[3-9] Thermal cycle The same operation as in the above step [3-5] was performed.

[3-10] Sequencing After gel filtration purification, it was subjected to sequencing using an automatic sequencer.

[3-11] Detection Result Table 1 also shows the identification result of the information DNA according to the above process. In the table, “◯” indicates that the sequence of the informational DNA contained in the leather can be easily identified by the above-described method, and “Δ” indicates that the number of amplifications by the PCR must be increased. This shows the sequence that could be identified.

[4] Evaluation of sedimentation properties of information-supported DNA-supported microparticles For each information-added DNA-added staining solution used in each of the above examples, the degree of sedimentation of the information-supported DNA-supported microparticles when left for 2 hours after stirring for 1 hour. Visual evaluation was made. The results are also shown in Table 1.
In the table, “◯” indicates that there is no sediment, “Δ” indicates that there is some sediment, and “x” indicates that there are many sediments.

  As is clear from the results shown in Table 1, it can be seen that the information nucleic acid can be identified in the information nucleic acid-containing leather material of the present invention. In addition, the information-containing nucleic acid-containing leather materials of Examples 1 to 12 and Example 18 are compared with the information-containing nucleic acid-containing leather materials of Examples 13 to 17 and Examples 19 to 20, and the information is obtained with fewer PCR amplifications. It can be seen that the activated nucleic acid can be identified. Further, the information nucleic acid-containing leather materials of Examples 1 to 15 and Example 17 are superior to the information nucleic acid-containing leather materials of Example 16 and Examples 18 to 20 without the precipitation of fine particles. Recognize.

As an example of DNA, there are structural formulas showing natural DNA (A) and DNA (B) derivatized at the 5 ′ position. FIG. 2 is a schematic diagram showing the structure of a DNA having primer-corresponding sites at both ends of an authentication information site as an example of an information nucleic acid used in the present invention. It is the flow schematic which shows an example of the detection procedure of the information-ized nucleic acid contained in the leather material of this invention.

Claims (8)

  An information nucleic acid-containing leather material comprising an information nucleic acid having a site having an arbitrary and known base sequence.   The information nucleic acid-containing leather material according to claim 1, wherein the content of the information nucleic acid is 0.5 to 500 µg with respect to 100 g of leather.   The information nucleic acid-containing leather material according to claim 1 or 2, wherein the information nucleic acid is added in a state of being supported on fine particles.   The information nucleic acid-containing leather material according to claim 3, wherein the fine particles have an average particle diameter of 0.01 to 1 µm.   The information nucleic acid-containing leather material according to claim 3 or 4, wherein the addition amount of the fine particles is 0.5 to 50 g with respect to 100 g of leather.   The method for producing an information nucleic acid-containing leather material according to any one of claims 1 to 5, wherein the solution containing the information nucleic acid is permeated into the material leather.   The method for producing an information nucleic acid-containing leather material according to claim 6, wherein the solution containing the information nucleic acid is a staining solution.   An information nucleic acid-containing leather product comprising the leather material according to any one of claims 1 to 5.

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