JP2006089473A - Alkaline bactericide - Google Patents

Alkaline bactericide Download PDF

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JP2006089473A
JP2006089473A JP2005244633A JP2005244633A JP2006089473A JP 2006089473 A JP2006089473 A JP 2006089473A JP 2005244633 A JP2005244633 A JP 2005244633A JP 2005244633 A JP2005244633 A JP 2005244633A JP 2006089473 A JP2006089473 A JP 2006089473A
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alkaline
bactericide
ethanol
bacteria
disinfectant
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Masahiro Okajima
眞裕 岡島
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AI SYSTEM PRODUCT KK
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AI SYSTEM PRODUCT KK
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Abstract

<P>PROBLEM TO BE SOLVED: To obtain an alkaline bactericide having immediate and lasting bactericidal effects. <P>SOLUTION: This alkaline bactericide is obtained by mixing ionized alkaline water with ethanol at a weight ratio of 0.9:0.1. As a result, after 0.5 min, all of Escherichia coli, Pseudomonas aeruginosa and salmonellas are not detected. The bactericide has immediate and lasting bactericidal effects on these bacteria. The ionized alkaline water composing the alkaline bactericide is mild to the human skin and, as the concentration of ethanol for mixing is about 10% and is same as alcohol for beverage use, so the skin is not affected. As the bactericidal activity is exerted immediately and lastingly, the human skin is immediately and lastingly sterilized without being damaged. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

本発明は、アルカリ性殺菌剤に関する。   The present invention relates to an alkaline disinfectant.

本発明では、殺菌の効果について即効性があり、かつ、持続性のあるアルカリ性殺菌剤を提供することを目的とする。   An object of the present invention is to provide an alkaline disinfectant that has an immediate effect on the effect of disinfection and that is durable.

上述した問題点を解決するためになされた請求項1に記載されたアルカリ性殺菌剤は、アルカリ性殺菌剤において、アルカリイオン水とエタノールとを、0.97:0.03〜0.80:0.20の重量比で混合したことを特徴とする。このような比率で作成されたアルカリ性殺菌剤は、即効性があり、かつ、持続性のある殺菌性能を発揮する。   The alkaline disinfectant according to claim 1, which has been made in order to solve the above-mentioned problems, is an alkaline disinfectant, wherein alkaline ionized water and ethanol are used in an amount of 0.97: 0.03 to 0.80: 0. It is characterized by mixing at a weight ratio of 20. The alkaline disinfectant prepared at such a ratio has an immediate effect and exhibits a sustained disinfecting performance.

請求項2に記載したように、アルカリイオン水とエタノールとを0.95:0.05〜0.85:0.15の重量比で混合したもののほうが好ましく、さらに請求項3に記載したように、アルカリイオン水とエタノールとを0.9:0.1の重量比で混合したものがもっとも好ましい。   As described in claim 2, it is preferable to mix alkaline ionized water and ethanol in a weight ratio of 0.95: 0.05 to 0.85: 0.15, and further as described in claim 3. A mixture of alkali ion water and ethanol in a weight ratio of 0.9: 0.1 is most preferable.

以下、本発明を実施するための最良の形態について図面を用いて説明する。
図1及び図2は、本実施形態のアルカリ性殺菌剤で殺菌の実験を行った実験結果の表である。
The best mode for carrying out the present invention will be described below with reference to the drawings.
FIG.1 and FIG.2 is a table | surface of the experimental result which conducted the sterilization experiment with the alkaline disinfectant of this embodiment.

本実施形態のアルカリ性殺菌剤は、アルカリイオン水とエタノールとを、0.9:0.1の重量比で混合したものである。アルカリイオン水は、極めて低濃度の電解質を含有した水を、隔膜を設けた電解槽において電気分解をすることによって、陰極室に生成するアルカリ水である。   The alkaline disinfectant of this embodiment is a mixture of alkaline ionized water and ethanol at a weight ratio of 0.9: 0.1. Alkaline ion water is alkaline water produced in the cathode chamber by electrolyzing water containing an extremely low concentration of electrolyte in an electrolytic cell provided with a diaphragm.

以下、本実施形態のアルカリ性殺菌剤の殺菌能力を証明する実験を行ったので、詳細に説明する。
試験は、財団法人日本食品分析センターで行った。
1)試験1
試験の概要 検体である本実施形態のアルカリ性殺菌剤(以下試験体と呼ぶ)に黄色ブドウ球菌及びメキシリン耐性黄色ブドウ球菌(以下「MRSA」)の菌液をそれぞれ添加、混合後、25℃で保存し、0.5、1及び10分間並びに、3、6、及び24時間保存後に生菌数を測定した。なお、試験液をSCDLP培地で10倍に希釈することにより、検体の影響を受けずに生菌数が測定できることを予備試験により確認した。
Hereinafter, since the experiment which proves the disinfection ability of the alkaline disinfectant of this embodiment was conducted, it explains in detail.
The test was conducted at the Japan Food Analysis Center.
1) Test 1
Outline of the test The bacterial solutions of Staphylococcus aureus and mexillin-resistant Staphylococcus aureus (hereinafter referred to as “MRSA”) were added to the alkaline bactericidal agent (hereinafter referred to as “test body”) of the present embodiment, which is a specimen, respectively, and then stored at 25 ° C. The viable cell counts were measured after storage for 0.5, 1 and 10 minutes and 3, 6, and 24 hours. In addition, it was confirmed by a preliminary test that the viable cell count can be measured without being influenced by the specimen by diluting the test solution 10 times with the SCDLP medium.

結果は図1に示す表1のように、黄色ブドウ球菌については、6時間後以後検出されなかった。MRSAについては、3時間後以後検出されなかった。尚、図1では比較対照として精製水に上記菌を混合したものについての生菌数も記載してある。   As shown in Table 1 of FIG. 1, the results were not detected after 6 hours for Staphylococcus aureus. MRSA was not detected after 3 hours. In addition, in FIG. 1, the number of living bacteria about what mixed the said microbe with purified water as a comparison control is also described.

これらの菌に対しては、持続的に殺菌力を発揮する。
2)試験2
試験の概要 検体を試験液とした。試験液に大腸菌、緑黄菌及びサルモネラ菌液をそれぞれ添加、混合後、25℃で保存し、0.5、1及び10分間並びに、3、6、及び24時間保存後に生菌数を測定した。なお、試験液をSCDLP培地で10倍に希釈することにより、検体の影響を受けずに生菌数が測定できることを予備試験により確認した。
These bacteria are sterilized continuously.
2) Test 2
Outline of the test The specimen was used as a test solution. Escherichia coli, S. yellow and Salmonella were added to the test solution, mixed and stored at 25 ° C., and the number of viable cells was measured after storage for 0.5, 1 and 10 minutes, and 3, 6, and 24 hours. In addition, it was confirmed by a preliminary test that the viable cell count can be measured without being influenced by the specimen by diluting the test solution 10 times with the SCDLP medium.

結果は図2に示す表1のように、大腸菌、緑黄菌及びサルモネラ菌のすべてについて、0.5分後以後検出されなかった。尚、図2では比較対照として精製水に上記菌を混合したものについての生菌数も記載してある。   As shown in Table 1 shown in FIG. 2, the results were not detected after 0.5 minutes for all of E. coli, S. yellow and Salmonella. In addition, in FIG. 2, the number of living bacteria about what mixed the said microbe with purified water is also described as a comparison control.

これらの菌に対しては、殺菌に関する効果ついて即効性があるうえに、持続性もある。
以上述べたように、本実施形態のアルカリ性殺菌剤を構成するアルカリイオン水は、人の肌にやさしく、また、混合するエタノールも10%前後と、飲料用のアルコールと変わらない濃度なので、人体にかけても肌が痛まない。
These bacteria have an immediate effect on sterilization and are also persistent.
As described above, the alkaline ionized water constituting the alkaline disinfectant of this embodiment is gentle to human skin, and the ethanol to be mixed is about 10%, which is a concentration that is not different from alcohol for beverages. Even the skin does not hurt.

しかも、上述したように、特定の菌に対しては即効かつ持続的に殺菌力を発揮するので、人肌を荒らさず殺菌することができる。また、即効性がない菌であっても、一定時間後には殺菌され、持続的に殺菌力を発揮するので、これらの菌についても有用である。   Moreover, as described above, since it exhibits an immediate and continuous sterilizing power against specific bacteria, it can sterilize human skin without damaging it. In addition, even bacteria having no immediate effect are sterilized after a certain period of time and exert their sterilizing power continuously. Therefore, these bacteria are also useful.

本実施形態のアルカリ性殺菌剤は、病院の殺菌や、家庭での手洗い、クリーンルームや給食センター等での殺菌などに用いると有用である。
次に、本実施形態のアルカリ性殺菌剤についての抗菌活性性能に関する追加実験を行ったので、以下に説明する。
The alkaline disinfectant of this embodiment is useful when used for sterilization in hospitals, hand washing at home, sterilization in clean rooms, lunch centers, and the like.
Next, additional experiments regarding the antibacterial activity performance of the alkaline disinfectant of the present embodiment were performed, and will be described below.

[方法] 試験液として本実施形態のアルカリ性殺菌剤(アルカリ性イオン水:エタノール=0.9:0.1、0.02mol/L NaOH、滅菌蒸留水を用いた。前培養培地としてハートインフュージョン寒天(Heart infusion agar )斜面培地を作成した。これに黄色ブドウ球菌(Staphylococcus aureus、IFO 12732)、枯草菌(Bacillus subtilis、IFO 3134)、大腸菌(Escherichia coil、IFO 3972)、緑膿菌(Pseudomonas aeruginosa、IFO 13275)を播種し37℃、17時間培養した。培養後、滅菌生理食塩液を加えて菌を浮遊させ光電比色計を用いて107CFU/mLの菌液を調製した。この菌液500μLを各試験液50mLに加え、25℃で撹搾しながらインキュベートした。そして、これら各試験液について、経時的(0.5、1、3、6、12時間毎)にサンプリングした。この際、BSゼラチン(Buffered saline with gelatin)を用いて希釈(10-1、2×10-2、10-2倍)した。サンプリング原液および希釈液を各3枚ずつハートインフュージョン寒天平面培地に播種し37℃、17時間培養後、コロニー数をカウントした。 [Method] The alkaline fungicide of this embodiment (alkaline ionized water: ethanol = 0.9: 0.1, 0.02 mol / L NaOH, sterilized distilled water was used as a test solution. Heart infusion agar ( Heart infusion agar was prepared, and S. aureus (Staphylococcus aureus, IFO 12732), Bacillus subtilis (IFO 3134), E. coli (Escherichia coil, IFO 3972), Pseudomonas aeruginosa, IFO 13275) and cultivated for 17 hours at 37 ° C. After culturing, sterilized physiological saline was added to suspend the bacteria, and a 10 7 CFU / mL bacterial solution was prepared using a photoelectric colorimeter. Was added to 50 mL of each test solution and incubated while being stirred at 25 ° C. Each of these test solutions was sampled over time (every 0.5, 1, 3, 6, 12 hours). BS gelatin (Buffered saline w (10 −1 , 2 × 10 −2 , 10 −2 times) The seeding stock solution and the diluted solution were inoculated on a heart infusion agar flat medium and cultured at 37 ° C. for 17 hours. Thereafter, the number of colonies was counted.

[結果および考察] 図3、図4、図5はそれぞれ本実施形態のアルカリ性殺菌剤、0.02mol/L NaOH、減菌蒸留水を適用後の細菌数の対数を、時間に対してプロットしたグラフを示す。アルカリ性殺菌剤は0.02mol/L NaOHと比較して細菌数の減少は緩やかであるものの、減菌蒸留水と比較すると枯草菌(Bacillus subtilis)以外は細菌数の減少は著しかった。このことより、アルカリ性殺菌剤は他の殺菌作用のある試験液と比較しても強い殺菌作用があると言える。    [Results and Discussion] FIG. 3, FIG. 4, and FIG. 5 are graphs in which the logarithm of the number of bacteria after application of the alkaline disinfectant, 0.02 mol / L NaOH, and sterilized distilled water of this embodiment is plotted against time. Indicates. Alkaline fungicides showed a gradual decrease in the number of bacteria compared to 0.02 mol / L NaOH, but a significant decrease in the number of bacteria except for Bacillus subtilis compared to sterilized distilled water. From this, it can be said that the alkaline bactericidal agent has a strong bactericidal action even when compared with other test liquids having a bactericidal action.

以上本発明の一実施形態ついて説明したが、本発明はこの実施形態に何等限定されるものではなく、本発明の要旨を逸脱しない範囲において、種々なる態様で実施し得ることは勿論である。   Although one embodiment of the present invention has been described above, the present invention is not limited to this embodiment, and it is needless to say that the present invention can be implemented in various modes without departing from the gist of the present invention.

尚、上記実施形態のアルカリ性殺菌剤を構成するアルカリイオン水のpHは、11.5〜12.5であることが好ましく、12がもっとも好ましい。   In addition, it is preferable that the pH of alkaline ionized water which comprises the alkaline disinfectant of the said embodiment is 11.5-12.5, and 12 is the most preferable.

本実施形態のアルカリ性殺菌剤で殺菌の実験を行った実験結果の表である。It is a table | surface of the experimental result which performed the experiment of disinfection with the alkaline disinfectant of this embodiment. 本実施形態のアルカリ性殺菌剤で殺菌の実験を行った実験結果の表である。It is a table | surface of the experimental result which performed the experiment of disinfection with the alkaline disinfectant of this embodiment. アルカリ性殺菌剤を適用後の細菌数の対数を、時間に対してプロットしたグラフである。It is the graph which plotted the logarithm of the number of bacteria after applying an alkaline disinfectant with respect to time. 0.02mol/LNaOHを適用後の細菌数の対数を、時間に対してプロットしたグラフである。It is the graph which plotted the logarithm of the number of bacteria after applying 0.02 mol / L NaOH with respect to time. 減菌蒸留水を適用後の細菌数の対数を、時間に対してプロットしたグラフである。It is the graph which plotted the logarithm of the number of bacteria after applying sterilized distilled water with respect to time.

Claims (3)

アルカリ性殺菌剤において、アルカリイオン水とエタノールとを、0.97:0.03〜0.80:0.20の重量比で混合したことを特徴とするアルカリ性殺菌剤。 An alkaline disinfectant, wherein alkaline ionized water and ethanol are mixed at a weight ratio of 0.97: 0.03 to 0.80: 0.20. 請求項1記載のアルカリ性殺菌剤において、アルカリイオン水とエタノールとを0.95:0.05〜0.85:0.15の重量比で混合したことを特徴とするアルカリ性殺菌剤。 The alkaline disinfectant according to claim 1, wherein alkaline ionized water and ethanol are mixed at a weight ratio of 0.95: 0.05 to 0.85: 0.15. 請求項1,2のいずれかに記載のアルカリ性殺菌剤において、アルカリイオン水とエタノールとを0.9:0.1の重量比で混合したことを特徴とするアルカリ性殺菌剤。 The alkaline bactericide according to any one of claims 1 and 2, wherein alkaline ionized water and ethanol are mixed at a weight ratio of 0.9: 0.1.
JP2005244633A 2004-08-25 2005-08-25 Alkaline bactericide Pending JP2006089473A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010504279A (en) * 2006-07-14 2010-02-12 アーステック、エルエルシー Methods and compositions for treating substances
WO2022131338A1 (en) * 2020-12-16 2022-06-23 株式会社エー・アイ・システムプロダクト Virus inactivator

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000107265A (en) * 1998-10-06 2000-04-18 Tokuriki Honten Co Ltd Alkaline ion agent for sterilization and disinfection
JP2000247892A (en) * 1999-02-26 2000-09-12 Ikeda Bussan Kk Preparation composition
JP2002080374A (en) * 2000-09-05 2002-03-19 Hisamitsu Pharmaceut Co Inc Alkali ion water-containing medicine composition for mammal application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000107265A (en) * 1998-10-06 2000-04-18 Tokuriki Honten Co Ltd Alkaline ion agent for sterilization and disinfection
JP2000247892A (en) * 1999-02-26 2000-09-12 Ikeda Bussan Kk Preparation composition
JP2002080374A (en) * 2000-09-05 2002-03-19 Hisamitsu Pharmaceut Co Inc Alkali ion water-containing medicine composition for mammal application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010504279A (en) * 2006-07-14 2010-02-12 アーステック、エルエルシー Methods and compositions for treating substances
WO2022131338A1 (en) * 2020-12-16 2022-06-23 株式会社エー・アイ・システムプロダクト Virus inactivator

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