JP2006069940A - Composition for improving lowering of bloodstream of skin derived from stress, composition for improving skin function and food and beverage using the same composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for improving lowering of bloodstream of the skin and a composition for improving skin function each derived from stress, capable of effectively preventing and alleviating lowering of bloodstream of the skin and lowering of skin function derived from stress, free from adverse effect, being safe and capable of readily being administered and ingested and to provide foods and beverages using these compositions. <P>SOLUTION: The composition for improving lowering of bloodstream of the skin derived from stress comprises fermented material of milk obtained by fermenting milk containing Lactococcus lactis subsp. cremoris FC (FERM AP-20185) as an active ingredient. The composition for improving the skin function comprises the fermented material of the milk as an active ingredient. The foods or the beverage are each obtained by using these compositions. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention has a stress-derived skin blood flow reduction improving composition, a skin function improving composition, and a composition for improving skin function, which have an effect of improving stress-induced skin blood flow reduction and skin function improvement by oral administration. It relates to food and drink.

In modern society, people are exposed to various types of physical, chemical, psychological and social stress. In particular, since work and human relations tend to be complicated in recent years, the proportion of people who have psychological stress is high regardless of generations. However, continued exposure to such stress can adversely affect various organs throughout the body, resulting in severe psychosomatic disorders, depression, gastric ulcers, hypertension and the like. Currently, drugs used for the purpose of reducing stress or preventing or treating diseases caused by stress include, for example, anxiolytic drugs and sleeping drugs using benzodiazepine compounds. In recent years, oral administration compositions containing triterpenic acid (compounds such as actinide acid), which are beneficial for the improvement of stress-induced microcirculatory insufficiency, have also been proposed. It is said that it also has an effect | action (for example, refer patent document 1).
JP 2003-321369 A

  However, although these chemicals are relatively safe, they have habits and side effects, so it is not preferable to take them frequently against temporary stress in daily life, and use after taking them Suspending the rebound may cause rebound and even worsen the symptoms, which is not ideal.

  On the other hand, as the oral administration composition disclosed in Patent Document 1 above, demand for an oral administration composition that significantly expresses an action for improving skin function has been increasing in recent years. There is also a need to develop new compositions that are safe and easy to take and ingest.

  The present invention has been made in view of such circumstances, and can effectively prevent and reduce stress-induced skin blood flow reduction and skin function deterioration, and is safe without side effects and can be taken and ingested. It is an object of the present invention to provide an easy-to-stress composition for improving skin blood flow reduction, a composition for improving skin function, and food and drink using them.

  In order to achieve the above-mentioned object, the present invention includes a fermented milk product containing Lactococcus lactis subsp. Cremoris FC, FERM AP-20185, which is fermented by containing Lactococcus lactis subsp. As a first gist, a composition for improving the reduction in skin blood flow derived from stress, containing Lactococcus lactis subsp. Cremoris FC, FERM AP- The composition for improving skin function containing the fermented milk product containing the fermented milk as an active ingredient is contained in the second gist, and the composition described in the first and second gist is used. The food and drink is the third gist.

  That is, the present inventors have intensively studied to solve the above problems. In the course of the research, milk was fermented with a special strain (Lactococcus lactis subsp. Cremoris FC) deposited by the present applicant as a kind of lactic acid bacteria, and thus obtained When a fermented milk is regularly ingested by subjects suffering from stress and subjects with decreased skin function or skin dysfunction, it has an improvement effect that could not be seen with normal ingestion of fermented milk. I found out. And, by various animal experiments, etc., the oral administration of the composition comprising the above milk fermented product as an active ingredient can significantly prevent or reduce stress-induced skin blood flow reduction or skin function reduction without causing side effects. Since this data and foods and drinks using the composition were applied to stress-induced skin blood flow reduction improvement applications and skin function improvement applications, The inventors have found that the intended purpose can be achieved without causing side effects, and have reached the present invention.

  As described above, the composition for improving skin blood flow reduction and the skin function improving composition derived from stress of the present invention contains lactococcus lactis subsp. Cremoris FC (Lactococcus lactis subsp. Cremoris) in milk. FC) is contained and fermented milk fermented product is contained as an active ingredient. Therefore, there are no side effects and habits unlike conventional chemicals, and it is possible to effectively prevent and reduce stress-induced skin blood flow reduction and skin function deterioration. Furthermore, even in foods and drinks made using this composition, since it becomes a functional food having an effect of improving the reduction of skin blood flow and skin function due to stress, it is excellent in daily health maintenance, etc. Show the effect.

  In particular, the above-mentioned fermented milk, together with Lactococcus lactis subsp. If it contains at least one kind of bacteria selected from the group consisting of subsp. thermophilus and is fermented, various functions as the product of the present invention will be expressed more significantly.

  In addition, when the milk is soy milk, the characteristics of soy milk itself act synergistically, and the stress-derived skin blood flow reduction improving effect is more significantly expressed.

  Furthermore, when the milk is milk, the skin function improving action of the product of the present invention is more significantly expressed.

  Next, an embodiment of the present invention will be described.

  As described above, the composition for improving skin blood flow reduction and the skin function improving composition derived from stress of the present invention contains lactococcus lactis subspices cremolith FC (Lactococcus lactis subsp. cremoris FC) is contained and fermented milk product obtained by fermentation is contained as an active ingredient. The “stress-derived skin blood flow reduction improving effect” and “skin function improving action” as used in the present invention are related to both actions when the skin function reduction is caused by stress-derived skin blood flow reduction, for example. However, even if there is no such relationship, the product of the present invention promotes the action of both, and can be applied in each application. Therefore, the above-mentioned “stress-derived skin blood flow reduction improving effect” and “skin function improving effect” are not synonymous and indicate individual actions depending on circumstances.

  The milk is not particularly limited, and, for example, animal-type products such as cow's milk (which may be skim milk powder) and plant-type products such as soy milk are used. These may be used alone or in combination of two or more. In addition, the said soymilk can be obtained by processing these by the conventionally well-known method, for example using the whole soybeans containing the fats and oils, molting soybeans, flake soybeans, etc. as the raw material.

  Here, when the product of the present invention is used for improving skin function, it is preferable to use milk as the milk because the skin function improving action can be expressed more significantly. On the other hand, when the product of the present invention is used for improving the reduction in skin blood flow derived from stress, soy milk can be used to improve the reduction in skin blood flow derived from stress more significantly as the milk. ,preferable.

  When only soy milk is used as the milk, it is difficult to promote fermentation as it is, so it is preferable to add lactose (lactose) at a ratio of about 5% by weight (hereinafter abbreviated as “%”). . Moreover, even if it is not the case where only soy milk is used as the said milk (For example, also when using only soy milk and milk, or using only milk), you may add lactose suitably. Furthermore, in addition to lactose, sugars such as glucose and fructose may be added to the milk as necessary.

  As a strain for fermenting the milk, Lactococcus lactis subsp. Cremoris FC, FERM AP-, which has been deposited by the applicant with the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, is incorporated. 20185) is used. This strain is different from ordinary Lactococcus lactis subspecies cremolith, and by using this strain, a composition having a remarkable stress-induced skin blood flow lowering effect and a skin function improving effect can be obtained. Obtainable. In addition, the said deposit strain has the characteristic activated at 10 degreeC or more, and makes 25-30 degreeC the optimal temperature. In addition, the deposited strain forms a colony having a white to milky white consistency on the medium. For example, when cultured on a BCP medium at 25 ° C. for 2 days, the deposited strain has a diameter of 0.5 to 1.0 mm. It becomes a circular (hemispherical) colony. Furthermore, there is no change in colony morphology due to culture conditions or physiological conditions, and there is no change in colony morphology due to mutation.

  If necessary, together with the deposited strain, for example, acetic acid bacteria belonging to the genus Acetobacter, Gluconacetobacter, etc., Lactobacillus acidphilus, Lactobacillus casei (Lactobacillus casei) ), Lactobacillus bulgaricus (Lactobacillus bulgaricus), Streptococcus salivarius subsp. Thermophilus, etc., may be used alone or in combination of two or more. In particular, together with the deposited strain, at least one selected from the group consisting of Acetobacter orientalis, Lactobacillus bulgaricus, and Streptococcus salivarius subsp. Thermophilus. What was obtained using these bacteria together is preferable because various functions as the product of the present invention can be expressed more significantly. Of these, those obtained by using Acetobacter orientalis together with the deposited strain are more preferable. In addition to the above deposited strain, the combination of Lactobacillus bulgaricus, Streptococcus salivarius, subspices, and thermophilus was used to improve the flavor not seen with other bacteria. This facilitates ingestion and is particularly advantageous for application to food and drink.

  The composition for improving stress-derived skin blood flow reduction or the composition for improving skin function according to the present invention can be produced, for example, as follows.

That is, first, about 1 g of the deposited strain [freeze-dried and powdered. The number of viable bacteria in 1 g of the powder is 1 × 10 ⁵ or more (preferably 1 × 10 ⁷ to 1 × 10 ⁹ )], and this is put in a sterilized culture bottle. If necessary, other bacteria (for example, Acetobacter orientalis etc.) are also added. In addition, these are not limited to a powdery thing. Then, sterilized milk (milk, soy milk, etc.) is prepared, and a predetermined amount (30-40 ml) thereof is added to the culture bottle. Moreover, saccharides, such as lactose, are added to the said milk as needed. Here, the order of each material put into the culture bottle may be different from the above. Then, the culture bottle is covered with a lid, shaken well, and then left at room temperature (15 to 30 ° C.) for 3 hours or longer, preferably 8 to 24 hours. In this way, the strain is activated and promotes fermentation. By subjecting the fermented milk product thus obtained as it is (in a live bacterial state) or by heat sterilization at about 85 ° C. for about 30 minutes, and further appropriately preparing it as an active ingredient, A composition (a composition for improving stress-derived skin blood flow reduction or a composition for improving skin function) can be obtained.

  The stress-derived skin blood flow reduction composition or skin function improving composition of the present invention may directly use the above-mentioned milk fermented product as it is (including heat-sterilized). Can be prepared by drying by freeze-drying or the like, or by mixing the powder with a suitable powder carrier. Alternatively, the powder may be tableted and granulated, or the powder may be encapsulated in a capsule. Further, the fermented milk product may be used by dissolving or dispersing in a suitable liquid carrier.

  In addition, the stress-derived skin blood flow reduction improving composition or the skin function improving composition of the present invention is not only for humans but also for animals such as pets and domestic animals. The effect is to be obtained, and the dosage is intended to be used to improve the target organism, the sex, weight, age, etc. It is set as appropriate according to the difference such as whether or not it is used, and not the improvement effect but the condition of using it for prevention. For example, for an adult, the effect of reducing stress-derived skin blood flow can be significantly obtained by administering the composition in several doses such that the composition is 60 to 600 g per day. On the other hand, the skin function improving effect can be significantly obtained by administering the composition in several batches so as to be 60 to 600 g per day. In animals such as pets and livestock, the above composition is preferably contained in an animal feed in a range of 3 to 5% and administered in a range of 1 to 500 g / kg body weight per day.

  On the other hand, the composition for improving stress-derived skin blood flow reduction or the composition for improving skin function of the present invention can be provided as a form in which the composition is associated with food or drink. Examples of the food and drink include health food, food for specified health use, soft drink, tea, jelly, yogurt, pudding, ice cream, popsicle, candy, chocolate, bread, cake, ham, meat sauce, curry, stew, Cheese, butter, dressing, etc.

  Next, examples will be described together with comparative examples.

  First, prior to Examples and Comparative Examples, powders of strains A to G shown in Table 1 below were prepared. “Lactococcus lactis subspecies cremoris FC” in the table is a strain (FERM AP-20185) deposited by the applicant at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology.

  In addition, HOS: HR-1 hairless mice (female, 10-12 weeks old) were prepared as experimental mice. These mice were transferred to a 26.5 cm × 15.5 cm × 12.5 cm height incubator around 6 weeks of age and acclimatized for 6 weeks at an environmental density of 5 animals (standard environment) per incubator. is there. Until the experiment, all mice were reared only with the basic feed (CLEA purified feed).

[Examples 1-8, Comparative Examples 1-6]
First, a sterilized culture bottle is prepared, and the strain powder of the above A is put therein, and further, sterilized milk (commercially available non-adjusted milk, non-fat milk solid content of 8.3% or more, milk fat content 3.5% or more) was prepared, and the milk (35 ml of milk per 1 g of the strain powder of A) was placed in the culture bottle. The culture bottle containing these was covered with a lid, shaken well, and then fermented under conditions of 25 ° C. × 12 to 15 hours to obtain a fermented milk product. And in Example 1, this milk fermented product was freeze-dried as it was, pulverized, and it mixed in the basic feed (CLEA refined feed, Nippon Claire Co., Ltd.) ratio of 3%. Moreover, in Example 2, after sterilizing the said fermented milk product at 85 ° C. for 30 minutes, it was freeze-dried and powdered, and this was mixed in the basic feed at a rate of 3%. In Comparative Example 1, milk was freeze-dried as it was to make powder, and this was mixed in the basic feed at a rate of 3%.

  On the other hand, in Example 3, instead of the milk of Example 1 above, soy milk (non-adjusted soy milk for business use, soy solid content of 10 to 11%) was used, and 5% lactose was added thereto to promote fermentation. A fermented milk was obtained. Then, using this fermented milk product, a mixed feed was prepared in the same manner as in Example 1. In accordance with this, in Example 4, soymilk: milk = 7: 3 was used to obtain a fermented milk product, and a mixed feed was prepared. In Example 5, soymilk: milk = 2: 10 was used to obtain a fermented milk and a mixed feed was prepared. In Comparative Example 2, the soymilk was freeze-dried as it was to obtain a powder, and this was mixed in the basic feed at a rate of 3%.

  On the other hand, in Example 6, it replaced with the strain A and obtained the fermented milk like Example 1 except using the strain B, and mixed feed was prepared using this. Accordingly, in Example 7, strain C was used to obtain a fermented milk product, and a mixed feed was prepared in the same manner. In Example 8, a strain D was used to obtain a fermented milk product, and a mixed feed was prepared in the same manner. In Comparative Example 3, strain E was used to obtain a fermented milk product, and a mixed feed was prepared in the same manner. In Comparative Example 4, strain F was used to obtain a fermented milk product, and a mixed feed was prepared in the same manner. In Comparative Example 5, strain G was used to obtain a fermented milk product, and a mixed feed was prepared in the same manner.

  Next, the previous experimental mouse was moved to an overcrowded environment (overcrowded stress load), and each of the feeds of Examples and Comparative Examples (Comparative Example 6 was a basic feed only) was reared for 7 days while allowing free intake. The overcrowded environment refers to a 26.5 cm x 15.5 cm x 12.5 cm incubator with a cross-shaped sill that divides the incubator into four equal parts, and 5 animals per frame ( (20 per incubator) refers to those that were bred with experimental mice. In the control group, the basic feed was continuously ingested in the standard environment (5 animals per incubator), and the animals were subsequently raised for 7 days.

  The experimental mice bred in this way were tested according to the following criteria and evaluated. The results are also shown in Tables 2 to 4 below.

[Blood flow]
The epidermal blood flow in mice was measured with a laser Doppler blood flow meter (Laser Doppler Perfusion Imager PIM II, manufactured by Lisca). This measurement was performed over time, 4 days and 7 days after the first day of the experiment (the first day when each individual feed was started). In addition, this measured value is shown by the ratio (%) calculated | required by contrast with this amount when the blood flow rate of a control group is set to 100.0.

[Epidermal moisture transpiration]
For the mice 7 days after the start of the experiment, the work of peeling the epidermis of the back (around the waist) with cellophane tape (tape stripping) was repeated 10 times. And the moisture transpiration | evaporation amount of the part (inflammatory part) which peeled off the epidermis was measured with time by the transpiration | evaporation measuring device (Thewameter TM210, product made by Curage & Khaza).

  From the results of the above “blood flow”, the mice of the examples were reared in an overcrowded environment and loaded with stress, and after 4 days of stress loading, a certain decrease in blood flow was observed compared to the initial stage. However, since the feed of the example was continued, the improvement effect was confirmed after 7 days of stress load. In addition, even if it sterilized the fermented milk like Example 2, the improvement effect was acquired significantly. From this, it can be judged that Example goods have the effect which improves the skin blood flow fall resulting from stress irrespective of a living microbe or a dead microbe. Further, in Examples, even when any of milk, soy milk, and a mixture thereof is used as a medium for obtaining the fermented milk product, the effect of improving the reduction of skin blood flow due to stress is significantly obtained. In particular, the effect of Example 3 using soymilk was conspicuous.

  On the other hand, the mice fed with the comparative diet showed a decrease in blood flow with each day due to stress, and no significant results were obtained. Further, even in Comparative Examples 3 to 5 using other Cremoris bacteria or lactic acid bacteria, the blood flow reduction improvement effect was not as significant as in the Examples.

  On the other hand, from the results of the above-mentioned “epidermal water transpiration”, the mice that continued to receive the diet of the example had higher skin regeneration ability than the mice that continued to receive the comparative diet, and the epidermal moisture transpiration due to tape stripping was higher. It can be seen that the increase was quickly suppressed (the recovery was quick). Further, in the examples, it was confirmed that the skin function improving effect was significantly obtained even when any of milk, soy milk, and a mixture thereof was used as a medium for obtaining the fermented milk product. In particular, the effect was remarkable in the examples using milk.

  The composition of the present invention and foods and drinks using the composition are preferably used for stress-derived skin blood flow reduction and skin function improvement applications, but also have immunostimulatory activity and intestinal regulation. Due to the fact that it contains a large amount of polysaccharides, it can be applied to immunostimulatory activity use and intestinal action use.

Claims (7)

  Lactococcus lactis subsp. Cremoris FC (FERM AP-20185) is contained in milk and is characterized by containing a fermented milk fermented product as an active ingredient. A composition for improving reduction in skin blood flow derived from stress.   Along with Lactococcus lactis sub-species Cremolis FC, the above milk fermented product is Acetobacter orientalis, Lactobacillus bulgaricus and Streptococcus salivarius subsp. 2. The composition for improving reduction in skin blood flow derived from stress according to claim 1, wherein the composition contains and fermented with at least one bacterium selected from the group consisting of thermophilus).   The composition for improving reduction in skin blood flow derived from stress according to claim 1 or 2, wherein the milk is soy milk.   Lactococcus lactis subsp. Cremoris FC (FERM AP-20185) is contained in milk and is characterized by containing a fermented milk fermented product as an active ingredient. A composition for improving skin function.   Along with Lactococcus lactis sub-species Cremolis FC, the above milk fermented product is Acetobacter orientalis, Lactobacillus bulgaricus and Streptococcus salivarius subsp. The composition for improving skin function according to claim 4, wherein the composition contains at least one bacterium selected from the group consisting of thermophilus and is fermented.   The composition for improving skin function according to claim 4 or 5, wherein the milk is milk.   A food or drink comprising the composition according to any one of claims 1 to 6.