JP2006045121A - Antibacterial composition - Google Patents
Antibacterial composition Download PDFInfo
- Publication number
- JP2006045121A JP2006045121A JP2004228602A JP2004228602A JP2006045121A JP 2006045121 A JP2006045121 A JP 2006045121A JP 2004228602 A JP2004228602 A JP 2004228602A JP 2004228602 A JP2004228602 A JP 2004228602A JP 2006045121 A JP2006045121 A JP 2006045121A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- oil
- antibacterial composition
- soluble licorice
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Non-Alcoholic Beverages (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
本発明は、抗菌性及び安全性に優れ、飲食品、医薬品、医薬部外品、及び化粧料のいずれかに好適に用いられる抗菌性組成物に関する。 The present invention relates to an antibacterial composition that is excellent in antibacterial properties and safety, and is suitably used for any of foods and drinks, pharmaceuticals, quasi drugs, and cosmetics.
従来より、微生物の繁殖による変質や腐敗を起こしやすい飲食品や化粧料等の保存性向上のために配合する抗菌剤としては、各種の合成品や天然物由来のものが使用されている。しかし、人の口や皮膚を経由して体内に入ることが多い飲食品や化粧料用途の抗菌剤としては合成品よりも安全性の高い天然物由来のものが好ましい。また、天然物由来のものであっても、可能な限り添加量を少なし、添加対象物の色、匂い、味、物性等に悪影響を及ぼさないものであることが望まれている。
このような微生物に対する有効な天然物系抗菌剤として、例えば、油溶性甘草抽出物が優れた抗菌作用を有することが知られている（非特許文献１及び非特許文献２参照）。しかし、油溶性甘草抽出物の添加濃度を上げると、その製品が着色したり、混濁してしまうという欠点がある。
Conventionally, various synthetic products and those derived from natural products have been used as antibacterial agents for improving the storage stability of foods and beverages and cosmetics that are prone to deterioration and spoilage due to the growth of microorganisms. However, antibacterial agents for foods and cosmetics that often enter the body via the human mouth and skin are preferably derived from natural products that are safer than synthetic products. Moreover, even if it is derived from a natural product, it is desired that the amount added is as small as possible and does not adversely affect the color, odor, taste, physical properties, etc. of the object to be added.
As an effective natural product antibacterial agent against such microorganisms, for example, it is known that an oil-soluble licorice extract has an excellent antibacterial action (see Non-Patent Document 1 and Non-Patent Document 2). However, when the additive concentration of the oil-soluble licorice extract is increased, there is a drawback that the product is colored or turbid.
また、緑茶抽出物が抗菌作用を有していることは知られており、また、緑茶抽出物が抗菌作用を示す濃度以下の濃度で抗生物質と併用することにより、メチシリン耐性黄色ブドウ状球菌（ＭＲＳＡ）等の薬剤耐性細菌類に対し、抗生物質の抗菌作用を回復させる作用を有することが報告されている（非特許文献３及び非特許文献４参照）。しかし、植物抽出物が抗菌作用を有する天然物との併用により、その天然物の抗菌性を増強させることは知られていない。 In addition, it is known that the green tea extract has an antibacterial action, and the methicillin-resistant Staphylococcus aureus ( It has been reported that it has an action of restoring the antibacterial action of antibiotics against drug resistant bacteria such as MRSA) (see Non-Patent Document 3 and Non-Patent Document 4). However, it is not known that plant extracts enhance the antibacterial properties of natural products when used in combination with natural products having antibacterial activity.
したがって消費者の自然指向及び安全指向の商品に対する需要増加を図る面から、抗菌剤の使用を必要最低限に抑えることができる抗菌性及び安全性に優れた代替組成物の開発が強く望まれているのが現状である。 Therefore, from the aspect of increasing demand for consumer-oriented and safety-oriented products, there is a strong demand for the development of an antibacterial and safe alternative composition that can minimize the use of antibacterial agents. The current situation is.
本発明は、前記従来における問題を解決し、以下の目的を達成することを課題とする。即ち、本発明は、油溶性甘草抽出物及びその精製物の抗菌作用を飛躍的に増強させることができ、飲食品や化粧品中の微生物に対して、少量の使用で優れた抗菌作用を示し、安全性に優れた抗菌性組成物を提供することを目的とする。 An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention can dramatically enhance the antibacterial action of the oil-soluble licorice extract and its purified product, and exhibits an excellent antibacterial action with a small amount of use against microorganisms in foods and beverages and cosmetics, An object is to provide an antibacterial composition excellent in safety.
前記課題を解決するため本発明者らが、油溶性甘草抽出物の抗菌作用を増強させることができる植物抽出物について鋭意検討を重ねた結果、没食子酸残基を有する化合物含有植物の抽出物が油溶性甘草抽出物及びその精製物の抗菌作用を飛躍的に増強させる作用を有することを知見した。 In order to solve the above-mentioned problems, the present inventors have conducted extensive studies on plant extracts that can enhance the antibacterial action of oil-soluble licorice extract. As a result, an extract of a compound-containing plant having a gallic acid residue is obtained. It has been found that the oil-soluble licorice extract and its purified product have the effect of dramatically enhancing the antibacterial action.
本発明は、本発明者らによる前記知見に基づくものであり、前記課題を解決するための手段としては、以下の通りである。即ち、
＜１＞ （Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種、並びに（Ｂ）没食子酸残基を有する化合物含有植物の抽出物を含有することを特徴とする抗菌性組成物である。
＜２＞ 油溶性甘草抽出物が、グリチルリーザ・グラブラ（Ｇｌｙｃｙｒｒｈｉｚａ ｇｌａｂｒａ）、グリチルリーザ・インフラータ（Ｇｌｙｃｙｒｒｈｉｚａ ｉｎｆｌａｔａ）及びグリチルリーザ・ウラレンシス（Ｇｌｙｃｙｒｒｈｉｚａ ｕｒａｌｅｎｓｉｓ）から選択される少なくとも１種の抽出物である前記＜１＞に記載の抗菌性組成物である。
＜３＞ 油溶性甘草抽出物の精製物が、グラブリジン、グラブレン及びリコカルコンＡのいずれかである前記＜１＞から＜２＞のいずれかに記載の抗菌性組成物である。
＜４＞ 没食子酸残基を有する化合物植物が、エピガロカテキンガレート含有植物及びガロタンニン含有植物から選択される少なくとも１種である前記＜１＞から＜３＞のいずれかに記載の抗菌性組成物である。
＜５＞ エピガロカテキンガレート含有植物が、ツバキ科カメリア属のチャ（Ｃａｍｅｌｌｉａ ｓｉｎｅｎｓｉｓ）である前記＜４＞に記載の抗菌性組成物である。
＜６＞ ツバキ科カメリア属のチャが、緑茶、ウーロン茶、及び紅茶のいずれかである前記＜５＞に記載の抗菌性組成物である。
＜７＞ ガロタンニン含有植物が、タラ、ユカン、ミロバラン、ウワウルシ、コウホネ、ヌルデ、ヨーロッパカエデ及びカシ類から選択される少なくとも１種である前記＜４＞に記載の抗菌性組成物である。
＜８＞ （Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種１質量部に対し（Ｂ）没食子酸残基を有する化合物含有植物の抽出物を０．０１〜１０００質量部含有する前記＜１＞から＜７＞のいずれかに記載の抗菌性組成物。
＜９＞ 飲食品、医薬品、医薬部外品、及び化粧料のいずれかに用いられる前記＜１＞から＜８＞のいずれかに記載の抗菌性組成物である。
The present invention is based on the above findings by the present inventors, and means for solving the above problems are as follows. That is,
<1> (A) containing at least one selected from an oil-soluble licorice extract and a purified product of the oil-soluble licorice extract, and (B) an extract of a compound-containing plant having a gallic acid residue. It is an antibacterial composition characterized.
<2> Oil-soluble licorice extract is an extract of Glycyrrhiza glabra , Glycyrrhiza inflata and Glycyrrhiza larensis 1 The antibacterial composition described.
<3> The antibacterial composition according to any one of <1> to <2>, wherein the purified product of the oil-soluble licorice extract is any one of grabrizine, grabrene, and lycochalcone A.
<4> The antibacterial composition according to any one of <1> to <3>, wherein the compound plant having a gallic acid residue is at least one selected from an epigallocatechin gallate-containing plant and a gallotannin-containing plant. It is.
<5> The antibacterial composition according to <4>, wherein the epigallocatechin gallate-containing plant is Camellia sinensis .
<6> The antibacterial composition according to <5>, wherein the Camellia family Camellia is one of green tea, oolong tea, and black tea.
<7> The antibacterial composition according to <4>, wherein the gallotannin-containing plant is at least one selected from cod, yukan, myrobalan, walrus, kohone, nulle, European maple, and oak.
<8> (A) An extract of a plant containing a compound having a gallic acid residue is added to 1 part by mass of at least one kind selected from an oil-soluble licorice extract and a purified product of the oil-soluble licorice extract. The antibacterial composition according to any one of <1> to <7>, containing 0.01 to 1000 parts by mass.
<9> The antibacterial composition according to any one of <1> to <8>, which is used in any of foods and drinks, pharmaceuticals, quasi drugs, and cosmetics.
本発明の抗菌性組成物は、（Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種、並びに（Ｂ）没食子酸残基を有する化合物含有植物の抽出物を含有することによって、両者が相乗的に作用して、油溶性甘草抽出物及びその精製物の使用量が少量であっても優れた抗菌性を有し、油溶性甘草抽出物の欠点である、着色、濁り、におい、味の悪影響を減少させることが可能となる。 The antibacterial composition of the present invention comprises (A) at least one selected from an oil-soluble licorice extract and a purified product of the oil-soluble licorice extract, and (B) extraction of a compound-containing plant having a gallic acid residue. By containing the product, both of them act synergistically, and have excellent antibacterial properties even when the amount of the oil-soluble licorice extract and its purified product used is small, due to the disadvantages of the oil-soluble licorice extract. Certain adverse effects of coloring, turbidity, odor and taste can be reduced.
本発明によると、従来における諸問題を解決でき、油溶性甘草抽出物及びその精製物の抗菌作用を飛躍的に増強させることができ、微生物に対して、少量の使用で優れた抗菌作用を示し、安全性に優れた抗菌性組成物を提供でき、飲食品、医薬品、医薬部外品、及び化粧料に好適に用いることができる。 According to the present invention, the conventional problems can be solved, the antibacterial action of oil-soluble licorice extract and its purified product can be dramatically increased, and it exhibits excellent antibacterial action against microorganisms even in a small amount. The antibacterial composition excellent in safety can be provided, and can be suitably used for foods and drinks, pharmaceuticals, quasi drugs, and cosmetics.
本発明の抗菌性組成物は、（Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種、並びに（Ｂ）没食子酸残基を有する化合物含有植物の抽出物を含有してなり、更に必要に応じてその他の成分を含有してなる。 The antibacterial composition of the present invention comprises (A) at least one selected from an oil-soluble licorice extract and a purified product of the oil-soluble licorice extract, and (B) extraction of a compound-containing plant having a gallic acid residue. In addition, it contains other components as necessary.
−油溶性甘草抽出物−
甘草は、マメ科Ｇｌｙｃｙｒｒｈｉｚａ属に属する多年生草本植物である。該甘草としては、中国・東北〜内蒙古〜西北地方などに産する東北甘草、西北甘草（Ｇｌｙｃｙｒｒｈｉｚａ ｕｒａｌｅｎｓｉｓ）、中国・新疆地方などに産する新疆甘草（脹果甘草、Ｇｌｙｃｙｒｒｈｉｚａ ｉｎｆｌａｔａ）、旧ソ連、アフガニスタン、トルコ、パキスタン、スペインなどに産するスペイン甘草（光果甘草、Ｇｌｙｃｙｒｒｈｉｚａ ｇｌａｂｒａ）などが挙げられる。これらの中でも、グリチルリーザ・グラブラ（Ｇｌｙｃｙｒｒｈｉｚａ ｇｌａｂｒａ）、グリチルリーザ・インフラータ（Ｇｌｙｃｙｒｒｈｉｚａ ｉｎｆｌａｔａ）、グリチルリーザ・ウラレンシス（Ｇｌｙｃｙｒｒｈｉｚａ ｕｒａｌｅｎｓｉｓ）が好ましい。
-Oil-soluble licorice extract-
Licorice is a perennial herbaceous plant belonging to the genus Glycyrrhiza . Examples of the licorice include Tohoku licorice, Glycyrrhiza uralensis produced in China, Tohoku to Inner Mongolia to the Northwest region, Xinjiang licorice (gulberry licorice, Glycyrrhiza inflata ) produced in China, Xinjiang, the former Soviet Union, , Spanish licorice ( Glycyrrhiza grabra) produced in Turkey, Pakistan, Spain and the like. Among these, glycyrrhiza grabra ( Glycyrrhiza glabra ), glycyrrhiza infrata ( Glycyrrhiza inflata ), and glycyrrhiza uralensis ( Glycyrrhiza uralensis ) are preferable.
前記甘草の根、根茎、葉、及び茎のいずれの部位も抽出原料として使用することができるが、これらの中でも、根及び根茎の少なくともいずれかが特に好ましい。また、抽出原料としては、生のものを使用しても乾燥させたものを使用してもよいが、工業的に製造されているグリチルリチンの抽出原料となっている乾燥根及び乾燥根茎、或いはグリチルリチンなどを得るために水で抽出した後の水抽出残渣を原料として使用することもできる。 Any of the roots, rhizomes, leaves and stems of the licorice can be used as an extraction raw material, and among these, at least one of the roots and rhizomes is particularly preferable. In addition, as raw materials for extraction, raw or dried ones may be used, but dry roots and dry rhizomes that are raw materials for industrially produced glycyrrhizin, or glycyrrhizin In order to obtain the above, a water extraction residue after extraction with water can be used as a raw material.
前記抽出に用いる有機溶媒としては、例えば、ベンゼン、トルエン、キシレン、エチルエーテル、メチルエチルケトン、メチルイソブチルケトン、ジクロロメタン、ジクロロエタン、クロロホルム、酢酸エチル、酢酸プロピル、酢酸ブチル、アセトン、メタノール、エタノール、プロパノール、含水メタノール、含水エタノール、含水プロパノールなどが挙げられる。更には、超臨界流体として二酸化炭素を用いることもできる。これらの有機溶媒の中では、エタノール又は含水エタノールを使用するのが食品衛生法上、問題が少ないため好ましい。 Examples of the organic solvent used for the extraction include benzene, toluene, xylene, ethyl ether, methyl ethyl ketone, methyl isobutyl ketone, dichloromethane, dichloroethane, chloroform, ethyl acetate, propyl acetate, butyl acetate, acetone, methanol, ethanol, propanol, water content Examples include methanol, hydrous ethanol, hydrous propanol and the like. Furthermore, carbon dioxide can also be used as the supercritical fluid. Among these organic solvents, it is preferable to use ethanol or hydrous ethanol because there are few problems in the Food Sanitation Law.
前記甘草又は甘草水抽出残渣から上述した有機溶媒で甘草油性抽出物を得るための条件は特に限定されるものではないが、標準的な方法を示すと、抽出原料に対し２〜１０倍量の有機溶媒を加え、攪拌しながら常温で抽出する方法及び加熱還流して抽出する方法がある。また、これらの方法をそれぞれ単独で、又は組み合わせて繰り返し操作すれば、抽出効率が向上し、より好ましい。 The conditions for obtaining the licorice oily extract from the licorice or licorice water extraction residue with the organic solvent described above are not particularly limited. There are a method of adding an organic solvent and extracting at room temperature while stirring, and a method of extracting by heating to reflux. In addition, it is more preferable to repeat these methods alone or in combination to improve extraction efficiency.
得られた抽出液は、遠心分離及び濾過により不溶物を取り除いた後、甘草油性抽出物として、そのまま使用することもできるし、更に常法により、濃縮して使用することもできる。また、適当な方法で抽出液を乾燥させれば、甘草油性抽出物として、黄褐色の抽出物粉末を得ることができる。
得られた液状抽出物をそのまま、又は液状抽出物を濃縮したもの、更には液状抽出物の粉末或いは固形の乾燥物が甘草油性抽出物として利用される。
The obtained extract can be used as it is as a licorice oily extract after removing insolubles by centrifugation and filtration, or can be further concentrated and used by a conventional method. If the extract is dried by an appropriate method, a tan extract powder can be obtained as a licorice oily extract.
The obtained liquid extract is used as it is, or the liquid extract is concentrated, and the powder of the liquid extract or the solid dried product is used as the licorice oily extract.
得られた油用性甘草抽出液は、該抽出液の希釈液若しくは濃縮液、該抽出液の乾燥物、又はこれらの粗精製物若しくは精製物を得るために、常法に従って希釈、濃縮、乾燥、精製等の処理を施してもよい。 The obtained oily licorice extract is diluted, concentrated and dried according to a conventional method to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crudely purified product or a purified product thereof. Further, treatment such as purification may be performed.
−油用性甘草抽出物の精製物−
前記油溶性甘草抽出物の精製物としては、抗菌性を有するグラブリジン、グラブレン及びリコカルコンＡのいずれかが好適に挙げられる。
前記精製物は、前記油溶性甘草抽出物を常法により精製することにより得ることができる。
前記精製方法としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、活性炭、多孔性樹脂を用いた方法、吸着剤を用いた方法、高速液体クロマトグラフィー（ＨＰＬＣ）などが挙げられる。
前記多孔性樹脂としては、芳香族系又は芳香族系修飾型のものが適当である。使用可能な多孔性樹脂の具体例としては、ダイヤイオンＨＰ，同２０，同２１、セパビーズＳＰ２０７，同８２５，同８５０（以上、三菱化学社製）、アンバーライトＸＡＤ２，同４，同７（以上、オルガノ社製）、等が挙げられる。
また、吸着剤としてはオクタデシルシリカゲル（ＯＤＳ）が好適に使用される。
-Purified product of oily licorice extract-
As the purified product of the oil-soluble licorice extract, any one of antibacterial grabrizine, grabrene and lycochalcone A is preferably mentioned.
The purified product can be obtained by purifying the oil-soluble licorice extract by a conventional method.
The purification method is not particularly limited and may be appropriately selected depending on the purpose. Examples thereof include activated carbon, a method using a porous resin, a method using an adsorbent, and high performance liquid chromatography (HPLC). Can be mentioned.
As the porous resin, those of aromatic type or aromatic type are suitable. Specific examples of usable porous resins include Diaion HP, 20, 21, Sepabead SP207, 825, 850 (above, manufactured by Mitsubishi Chemical Corporation), Amberlite XAD2, 4, 7, (above) , Manufactured by Organo Corporation), and the like.
Further, octadecyl silica gel (ODS) is preferably used as the adsorbent.
−没食子酸残基を有する化合物含有植物−
前記没食子酸残基を有する化合物含有植物としては、エピガロカテキンガレート含有植物及びガロタンニン含有植物から選択される少なくとも１種が挙げられる。
-Compound-containing plant having a gallic acid residue-
Examples of the compound-containing plant having a gallic acid residue include at least one selected from epigallocatechin gallate-containing plants and gallotannin-containing plants.
前記エピガロカテキンガレート含有植物は、ツバキ科カメリア属のチャ（Ｃａｍｅｌｌｉａ ｓｉｎｅｎｓｉｓ）が好適であり、該ツバキ科カメリア属のチャとしては、緑茶、ウーロン茶、紅茶、などが挙げられ、抽出原料としては、葉が用いられる。 The epigallocatechin gallate-containing plant is preferably Camellia sinensis tea, and examples of the Camellia camellia cha include green tea, oolong tea, black tea, etc. Leaves are used.
前記ガロタンニン含有植物としては、タラ、ユカン、ミロバラン、ウワウルシ、コウホネ、ヌルデ、ヨーロッパカエデ、及びカシ類から選択される少なくとも１種が好適である。
前記タラ（マメ科、Ｃａｅｓａｌｐｉｎｉａ ｓｐｉｎｏｓａ）の抽出原料としては、果皮、幹などが挙げられる。前記ヌルデ（ウルシ科、Ｒｈｕｓ ｊａｖａｎｉｃａ）の抽出原料としては、葉にできた虫こぶなどが挙げられる。前記カシ類（ブナ科、Ｑｕｅｒｃｕｓ ｉｎｆｅｃｔｏｒｉａ）の抽出原料としては、葉にできた虫こぶなどが挙げられる。前記ミロバラン（シクンシ科、Ｔｅｒｍｉｎａｌｉａ ｃｈｅｂｕｌａ）の抽出原料としては、果実なそが挙げられる。前記コウホネ（スイレン科、Ｎｕｐｈａｒ ｊａｐｏｎｉｃｕｍ）の抽出原料としては、葉などが挙げられる。前記ヨーロッパカエデ（カエデ科、Ａｃｅｒ ｐｌａｔａｎｏｉｄｅｓ）の抽出原料としては、葉などが挙げられる。前記ウワウルシ（ツツジ科、Ａｒｃｔｏｓｔａｐｈｙｌｏｓ ｕｖａ−ｕｒｓｉ）の抽出原料としては、葉などが挙げられる。前記ユカン（トウダイクサ科、Ｐｈｙｌｌａｎｔｈｕｓ ｅｍｂｌｉｃａ）の抽出原料としては、果実などが挙げられる。
As the gallotannin-containing plant, at least one selected from cod, yukan, myrobalan, walrus, kouhone, null de, European maple, and oak is preferable.
Examples of the raw material for extracting the cod (Leguminosae, Caesalpinia spinosa ) include pericarp and trunk. As an extraction raw material of the above-mentioned Nurde (Ruthaceae, Rhus javanica ), insect galling formed on a leaf can be mentioned. Examples of the raw material for extracting the oaks ( Quercus inferior ) include insect gallings formed on leaves. An example of an extraction raw material for the Myrobalan ( Terminalia chebula ) is fruit berry. Examples of the extraction raw material for the mulberry ( Nymphalaceae , Nufar japonicum ) include leaves. Examples of the extraction raw material for the European maple ( Acer platanoides ) include leaves. As an extraction raw material of the above-mentioned walrus (Azalea, Arctostaphylos uva-ursi ), a leaf etc. are mentioned. Examples of the extraction material for the Yukan (Phyperanthaceae, Phyllanthus emblica ) include fruits.
前記没食子酸残基を有する化合物含有植物から没食子酸残基を有する化合物を抽出する方法としては、特に制限はなく、目的に応じて適宜選択することができ、植物の抽出に一般に用いられている抽出方法により得ることができる。前記抽出物には、抽出液、該抽出液の希釈液を乾燥して得られる乾燥物、又はこれらの粗精製物もしくは精製物のいずれもが含まれる。 The method for extracting the compound having a gallic acid residue from the compound-containing plant having a gallic acid residue is not particularly limited and can be appropriately selected depending on the purpose, and is generally used for extracting a plant. It can be obtained by an extraction method. Examples of the extract include an extract, a dried product obtained by drying a diluted solution of the extract, and a crude product or a purified product thereof.
前記抽出に用いる溶媒としては、水若しくは親水性有機溶媒又はこれらの混合液を室温又は溶媒の沸点以下の温度で用いることが好ましい。
前記抽出溶媒として使用し得る水としては、例えば、純水、水道水、井戸水、鉱泉水、鉱水、温泉水、湧水、淡水等の他、これらに各種処理を施したものが含まれる。水に施す処理としては、例えば、精製、加熱、殺菌、ろ過、イオン交換、浸透圧の調整、緩衝化等が含まれる。従って、本発明において抽出溶媒として使用し得る水には、精製水、熱水、イオン交換水、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水等も含まれる。
As the solvent used for the extraction, it is preferable to use water, a hydrophilic organic solvent, or a mixture thereof at room temperature or a temperature not higher than the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.
前記親水性有機溶媒としては、例えば、メタノール、エタノール、プロピルアルコール、イソプロピルアルコール等の炭素数１〜５の低級アルコール；アセトン、メチルエチルケトン等の低級脂肪族ケトン；１，３−ブチレングリコール、プロピレングリコール、グリセリン等の炭素数２〜５の多価アルコールなどが挙げられ、これら親水性有機溶媒と水との混合溶媒などを用いることができる。 Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and a mixed solvent of these hydrophilic organic solvents and water can be used.
得られた抽出液は、該抽出液の希釈液若しくは濃縮液、該抽出液の乾燥物、又はこれらの粗精製物若しくは精製物を得るために、常法に従って希釈、濃縮、乾燥、精製等の処理を施してもよい。 The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.
前記（Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種１質量部に対し、前記（Ｂ）没食子酸残基を有する化合物含有植物の抽出物を０．０１〜１０００質量部含有することが好ましく、０．１〜１００質量部がより好ましく、１〜１００質量部が更に好ましい。 For (A) at least one kind selected from the oil-soluble licorice extract and the purified product of the oil-soluble licorice extract, (B) the extract of the compound-containing plant having a gallic acid residue is 0. It is preferable to contain 0.01-1000 mass parts, 0.1-100 mass parts is more preferable, and 1-100 mass parts is still more preferable.
本発明の抗菌性組成物には、前記（Ａ）油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種、並びに前記（Ｂ）没食子酸残基を有する化合物含有植物の抽出物以外にも、組成物の種類、剤型などに応じた公知のその他の成分を添加することができる。該その他の成分としては、例えば、界面活性剤、油脂類、アルコール類、増粘剤、キレート剤、ｐＨ調整剤、香料、色素、ビタミン類、アミノ酸類などが挙げられる。 The antibacterial composition of the present invention contains at least one selected from (A) the oil-soluble licorice extract and a purified product of the oil-soluble licorice extract, and (B) a compound having a gallic acid residue. In addition to the plant extract, other known components can be added according to the type and dosage form of the composition. Examples of the other components include surfactants, fats and oils, alcohols, thickeners, chelating agents, pH adjusters, fragrances, pigments, vitamins, amino acids, and the like.
本発明の抗菌性組成物が適用される微生物としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、細菌、真菌類、などが挙げられる。 There is no restriction | limiting in particular as microorganisms to which the antibacterial composition of this invention is applied, According to the objective, it can select suitably, For example, bacteria, fungi, etc. are mentioned.
前記細菌としては、例えば、グラム陽性菌、グラム陰性菌などが挙げられ、また、嫌気性菌、通性嫌気性菌、微好気性菌、好気性菌等のいずれであってもよい。該細菌の具体的な種としては、例えば、Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ、Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｐｙｏｇｅｎｅｓ、Ｅｎｔｅｒｏｃｏｃｃｕｓ ｆａｅｃａｌｉｓ、Ｂａｃｉｌｌｕｓ ａｎｔｈｒａｃｉｓ、Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｉｓ、Ｃｌｏｓｔｒｉｄｉｕｍ ｔｅｔａｎｉ、Ｌｉｓｔｅｒｉａ ｍｏｎｏｃｙｔｏｇｅｎｅｓ、Ｐｓｅｕｄｏｍｏｎａｓ ａｅｒｕｇｉｎｏｓａ、Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ、Ｈａｅｍｏｐｈｉｌｕｓ ｉｎｆｌｕｅｎｚａｅ、Ｎｅｉｓｓｅｒｉａ ｇｏｎｏｒｒｈｏｅａｅ、Ｍｙｃｏｂａｃｔｅｒｉｕｍ ｔｕｂｅｒｃｕｌｏｓｉｓ、Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ、Ｓｔｒｅｐｔｏｍｙｃｅｓ ａｎｔｉｂｉｏｔｉｃｕｓ、Ｓａｌｍｏｎｅｌｌａ ｔｙｐｈｉ、Ｅｄｗａｒｄｓｉｅｌｌａ ｔａｒｄａ、Ｃｉｔｒｏｂａｃｔｅｒ ｆｒｅｕｎｄｉｉ、Ｖｉｂｒｉｏ ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ、Ｍｏｒｇａｎｅｌｌａ ｍｏｒｇａｎｉｉ、Ｓｅｒａｔｉａ ｍａｒｃｅｓｃｅｎｓ、Ｋｌｅｂｓｉｅｌｌａ ｐｎｅｕｍｏｎｉａｅ、Ｓｈｉｇｅｌｌａ ｄｙｓｅｎｔｅｒｉａｅ、Ｙｅｒｓｉｎｉａ ｐｅｓｔｉｓ、Ｔｒｅｐｏｎｅｍａ ｐａｌｌｉｄｕｍ、Ｌｅｐｔｏｓｐｉｒａ ｉｎｔｅｒｒｏｇａｎｓｅ、Ｃａｍｐｙｌｏｂａｃｔｏｒ ｊｅｊｕｎｉ、Ｌａｃｔｏｂａｃｉｌｌｕｓ ｌａｔｃｉｓ、Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｍｕｔａｎｓ、Ｌａｃｔｏｂａｃｉｌｌｕｓ ｃａｓｅｉ等が挙げられる。 Examples of the bacterium include gram positive bacteria and gram negative bacteria, and may be any of anaerobic bacteria, facultative anaerobic bacteria, microaerobic bacteria, aerobic bacteria, and the like. Specific species of the bacterium, for example, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis, Bacillus anthracis, Bacillus subtilis, Clostridium tetani, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli, Haemophilus influenzae, Neisseria gonorrhoeae, Mycobacterium tuberculosis, Corynebacterium glutamicum , Streptomyces antibiotics , Salm onella typhi, Edwardsiella tarda, Citrobacter freundii , Vibrio parahaemolyticus, Morganella morganii, Seratia marcescens, Klebsiella pneumoniae, Shigella dysenteriae, Yersinia pestis, Treponema pallidum, Leptospira interroganse, Campylobactor jejuni, Lactobacillus latcis, Streptococcus mutans, Lactobacillus casei and the like.
前記真菌類としては、大きく酵母と糸状菌に分類することができる。前記酵母としては、例えば、Ｃａｎｄｉｄａ ａｌｂｉｃａｎｓ、Ｃ． ｇｌａｂｒａｔａ、Ｃ． ｔｒｏｐｉｃａｌｉｓ、Ｃ． ｐａｒａｐｓｉｌｏｓｉｓ、Ｃ． ｓｔｅｌｌａｔｏｉｄｅａ、Ｃｒｙｐｔｏｃｏｃｃｕｓ ｎｅｏｆｏｒｍａｎｓ、Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉｓｉａｅ等が挙げられる。前記糸状菌としては、例えば、Ａｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ、Ｃｌａｄｏｓｐｏｒｉｕｍ ｃｌａｄｏｓｐｏｒｉｏｉｄｅｓ、Ｐａｅｃｉｌｏｍｙｃｅｓ ｖａｒｉｏｔｉｉ、Ｅｕｒｏｔｉｕｍ ｓｐ．、Ｐｅｎｉｃｉｌｌｉｕｍ ｓｐ．等が挙げられる。 The fungi can be broadly classified into yeast and filamentous fungi. Examples of the yeast include Candida albicans , C.I. glabrata , C.I. tropicalis , C.I. parapsilosis , C.I. Stellatoidea , Cryptococcus neoformans , Saccharomyces cerevisiae and the like. Examples of the filamentous fungi include Aspergillus niger , Cladosporum cladosporoides , Paecilomyces variotii , and Eurotium sp. , Penicillium sp. Etc.
本発明の抗菌性組成物は、飲食品、医薬品、医薬部外品、及び化粧料のいずれかに配合して、保存性を向上させることができる。なお、本発明の抗菌性組成物には、一般に用いられている抗菌剤、防腐剤、鮮度保持剤、保存剤、酸化防止剤、日持ち向上剤などを合わせて配合することができる。 The antibacterial composition of the present invention can be blended in any of foods and drinks, pharmaceuticals, quasi drugs, and cosmetics to improve storage stability. In addition, generally used antibacterial agents, preservatives, freshness-preserving agents, preservatives, antioxidants, shelf life improvers and the like can be blended in the antibacterial composition of the present invention.
前記飲食品としては、特に制限はなく、目的に応じて適宜選定することができるが、例えば、清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等の飲料；アイスクリーム、アイスシャーベット、かき氷等の冷菓；そば、うどん、はるさめ、ぎょうざの皮、しゅうまいの皮、中華麺、即席麺等の麺類；飴、キャンディー、ガム、チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子、パン等の菓子類；カニ、サケ、アサリ、マグロ、イワシ、エビ、カツオ、サバ、クジラ、カキ、サンマ、イカ、アカガイ、ホタテ、アワビ、ウニ、イクラ、トコブシ等の水産物；かまぼこ、ハム、ソーセージ等の水産・畜産加工食品；加工乳、発酵乳等の乳製品；サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等の油脂及び油脂加工食品；ソース、たれ等の調味料；カレー、シチュー、親子丼、お粥、雑炊、中華丼、かつ丼、天丼、うな丼、ハヤシライス、おでん、マーボドーフ、牛丼、ミートソース、玉子スープ、オムライス、餃子、シューマイ、ハンバーグ、ミートボール等のレトルトパウチ食品；種々の形態の健康・栄養補助食品；錠剤、カプセル剤、ドリンク剤、トローチ等の医薬品、医薬部外品などが挙げられる。なお、前記飲食物は上記例示に限定されるものではない。 There is no restriction | limiting in particular as said food-drinks, Although it can select suitably according to the objective, For example, drinks, such as a soft drink, a carbonated drink, a nutrient drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice Noodles such as buckwheat noodles, udon, harusame, gyoza skin, Chinese noodles, instant noodles, etc .; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery Confectionery such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallops, abalone, sea urchin, crabs, tocobushi, etc .; Fishery and livestock processed foods such as sausages; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, show Oils and processed oils such as ning, whipped cream and dressing; seasonings such as sauces and sauces; curry, stew, oyakodon, rice bowl, miscellaneous foods, Chinese rice bowl, and rice cake, tempura, eel rice cake, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburg, meatballs; various forms of health and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. Goods. In addition, the said food / beverage is not limited to the said illustration.
前記化粧料としては、例えば、軟膏、クリーム、乳液、ローション、パック、ゼリー、リップクリーム、口紅、入浴剤、トニック、リンス、シャンプー、アストリンゼント、などが挙げられる。 Examples of the cosmetics include ointments, creams, emulsions, lotions, packs, jellies, lip balms, lipsticks, bath agents, tonics, rinses, shampoos, and astringents.
以下、本発明の実施例を説明するが、本発明は、これらの実施例に何ら限定されるものではない。 Examples of the present invention will be described below, but the present invention is not limited to these examples.
（製造例１）
−油溶性甘草抽出物Ａの製造−
アフガニスタン産甘草（Ｇｌｙｃｙｒｒｈｉｚａ ｇｌａｂｒａ）１０００ｇを５ｃｍ程度の大きさに切断した後フラスコに取り、メタノール５０００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下で濃縮した後、減圧乾燥を行って、油溶性甘草抽出物Ａを１９５ｇ得た。
(Production Example 1)
-Production of oil-soluble licorice extract A-
After 1,000 g of Afghanistan licorice ( Glycyrrhiza glabra ) was cut into a size of about 5 cm, it was taken in a flask, 5000 ml of methanol was added, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 195 g of oil-soluble licorice extract A.
（製造例２）
−グラブリジンの製造−
製造例１で得られた抽出物１００ｇを、シリカゲル、ＯＤＳカラムクロマトグラフィーを繰り返し行った後、再結晶を行って、グラブリジンを１．０ｇ得た。
(Production Example 2)
-Production of grabrizine-
100 g of the extract obtained in Production Example 1 was repeatedly subjected to silica gel and ODS column chromatography, and then recrystallized to obtain 1.0 g of grabridine.
（製造例３）
−油溶性甘草抽出物Ｂの製造−
中国・新疆産甘草（Ｇｌｙｃｙｒｒｈｉｚａ ｉｎｆｌａｔａ）１０００ｇを１ｃｍ程度の大きさに切断後フラスコに取り、酢酸エチル５０００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下で濃縮した後、減圧乾燥を行って、油溶性甘草抽出物Ｂを９３ｇ得た。
(Production Example 3)
-Production of oil-soluble licorice extract B-
1000 g of licorice from China / Xinjiang ( Glycyrrhiza inflata ) was cut into a size of about 1 cm, taken into a flask, added with 5000 ml of ethyl acetate, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 93 g of oil-soluble licorice extract B.
（製造例４）
−リコカルコンＡの製造−
製造例３で得られた抽出物５０ｇを、シリカゲル、ＯＤＳカラムクロマトグラフィーを繰り返し行った後、再結晶を行って、リコカルコンＡを２．１ｇ得た。
(Production Example 4)
-Manufacture of Ricochalcone A-
50 g of the extract obtained in Production Example 3 was repeatedly subjected to silica gel and ODS column chromatography, and then recrystallized to obtain 2.1 g of lycochalcone A.
（製造例５）
−油溶性甘草抽出物Ｃの製造−
中国・東北産甘草（Ｇｌｙｃｙｒｒｈｉｚａ ｕｒａｌｅｎｓｉｓ）１００ｇを粉砕後フラスコに取り、塩化メチレン５００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下濃縮した後、減圧乾燥を行って、油溶性甘草抽出物Ｃを５ｇ得た。
(Production Example 5)
-Production of oil-soluble licorice extract C-
100 g of licorice from Tohoku, China ( Glycyrrhiza uralensis ) was pulverized, placed in a flask, added with 500 ml of methylene chloride, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 5 g of oil-soluble licorice extract C.
（製造例６）
−緑茶抽出物の製造−
緑茶１００ｇを粉砕後フラスコに取り、水５００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下濃縮した後、減圧乾燥を行って、緑茶抽出物３０ｇを得た。
(Production Example 6)
-Manufacture of green tea extract-
100 g of green tea was pulverized, placed in a flask, added with 500 ml of water, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 30 g of a green tea extract.
（製造例７）
−タラ抽出物の製造−
タラの幹１００ｇを粉砕後フラスコに取り、５０％エタノール水溶液５００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下濃縮した後、減圧乾燥を行って、タラ抽出物２８ｇを得た。
(Production Example 7)
-Manufacture of cod extract-
After crushing 100 g of the cod trunk, it was taken into a flask, 500 ml of a 50% ethanol aqueous solution was added, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 28 g of cod extract.
（製造例８）
−ヌルデ抽出物の製造−
ヌルデの虫こぶ１００ｇを粉砕後フラスコに取り、７０％アセトン水溶液５００ｍｌを加えて、８０℃で２時間抽出した後、ろ過した。得られたろ液を減圧下濃縮した後、減圧乾燥を行い、ヌルデ抽出物３３ｇを得た。
(Production Example 8)
-Production of Nurde extract-
After pulverizing 100 g of Nurde's insect hump, it was taken into a flask, 500 ml of 70% acetone aqueous solution was added, extracted at 80 ° C. for 2 hours, and then filtered. The obtained filtrate was concentrated under reduced pressure and then dried under reduced pressure to obtain 33 g of a null de extract.
（実施例１）
−抗菌活性の測定方法−
製造例１，３，５の３種の油溶性甘草抽出物Ａ〜Ｃ、製造例２のグラブリジン、及び製造例４のリコカルコンＡ、並びに製造例６〜８の没食子酸残基を有する化合物含有植物の抽出物について、下記の微生物に対する抗菌活性についてブレインハートインヒュージョン寒天培地を用いた寒天培地希釈法による最小発育阻止濃度（ＭＩＣ）で評価した。培養温度は３０℃、培養時間は４８時間とし、発育の有無を肉眼で判断した。結果を表１に示す。なお、表１中の数値はＭＩＣ（μｇ／ｍＬ）であり、抗菌活性が強いほどこの数値は小さくなる。
Example 1
-Measurement method of antibacterial activity-
Three kinds of oil-soluble licorice extracts A to C of Production Examples 1, 3, and 5, Grabrizine of Production Example 2, Lycochalcone A of Production Example 4, and a compound-containing plant having a gallic acid residue of Production Examples 6 to 8 The antibacterial activity against the following microorganisms was evaluated by the minimum inhibitory concentration (MIC) by the agar medium dilution method using brain heart infusion agar medium. The culture temperature was 30 ° C., the culture time was 48 hours, and the presence or absence of growth was judged with the naked eye. The results are shown in Table 1. In addition, the numerical value of Table 1 is MIC (microgram / mL), and this numerical value becomes small, so that antibacterial activity is strong.
＜試験微生物＞
Ａ．スタヒロコッカス アウレウス（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）
Ｂ．バチルス ズブチリス（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｉｓ）
Ｃ．ストレプトコッカス ミュータンス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｍｕｔａｎｓ）
Ｄ．ラクトバチルス カゼイ（Ｌａｃｔｏｂａｃｉｌｌｕｓ ｃａｓｅｉ）
<Test microorganism>
A. Staphylococcus aureus
B. Bacillus subtilis (Bacillus subtilis)
C. Streptococcus mutans ( Streptococcus mutans )
D. Lactobacillus casei ( Lactobacillus casei )
＜抗菌活性試験結果＞
これに対し、製造例６〜８の没食子酸残基を有する化合物含有植物の抽出物の抗菌活性は弱く、緑茶抽出物の最小発育阻止濃度（ＭＩＣ）が４００μｇ／ｍＬを示した以外には抗菌活性は認められなかった。
<Antimicrobial activity test results>
On the other hand, the antibacterial activity of the extracts of the compounds-containing plants having gallic acid residues in Production Examples 6 to 8 was weak and the antibacterial activity except that the minimum inhibitory concentration (MIC) of the green tea extract was 400 μg / mL. No activity was observed.
（実施例２）
−油溶性甘草抽出物及び該油溶性甘草抽出物の精製物から選択される少なくとも１種と没食子酸残基を有する化合物含有植物の抽出物との併用による抗菌活性の試験−
製造例１，３，５の３種の油溶性甘草抽出物Ａ〜Ｃ、製造例２のグラブリジン、及び製造例４のリコカルコンＡ、並びに製造例６〜８の没食子酸残基を有する化合物含有植物の抽出物についての抗菌相乗効果を実施例１と同様にして調べた。結果を表２〜表１３に示す。なお、表２〜表４は、スタヒロコッカス アウレウスに対するＭＩＣの結果、表５〜表７は、バチルス ズブチリスに対するＭＩＣの結果、表８〜表１０は、ストレプトコッカス ミュータンスに対するＭＩＣの結果、表１１〜表１３は、ラクトバチルス カゼイに対するＭＩＣの結果をそれぞれ示す。
(Example 2)
-Test of antibacterial activity by combined use of an extract of a plant containing a compound having a gallic acid residue and at least one selected from an oil-soluble licorice extract and a purified product of the oil-soluble licorice extract-
Three kinds of oil-soluble licorice extracts A to C of Production Examples 1, 3, and 5, Grabrizine of Production Example 2, Lycochalcone A of Production Example 4, and a compound-containing plant having a gallic acid residue of Production Examples 6 to 8 The antibacterial synergistic effect of the extract of was examined in the same manner as in Example 1. The results are shown in Tables 2 to 13. Tables 2 to 4 show the MIC results for Staphylococcus aureus, Tables 5 to 7 show the MIC results for Bacillus subtilis, Tables 8 to 10 show the MIC results for Streptococcus mutans, Tables 11 to 11 Table 13 shows the MIC results for Lactobacillus casei, respectively.
表２〜表１３の結果から、油溶性甘草抽出物と没食子酸残基を有する化合物含有植物の抽出物とを併用した場合には、油溶性甘草抽出物のＭＩＣ＝３．１３〜２５μｇ／ｍＬと顕著な抗菌作用を示し、油溶性甘草抽出物単独の場合のＭＩＣ＝２００〜４００μｇ／ｍＬを大幅に低下させることができる。また、油溶性甘草抽出物の精製物であるグラブリジン及びリコカルコンＡについても、これらの単独の場合のＭＩＣ＝１２．５〜２５μｇ／ｍＬであったが、没食子酸残基を有する化合物含有植物の抽出物とを併用した場合は、ＭＩＣ＝３．１３〜６．２５μｇ／ｍＬであった。
従って、油溶性甘草抽出物及びその精製物と没食子酸残基を有する化合物含有植物の抽出物とを併用することによって、顕著な抗菌作用の相乗効果を有することが認められた。
From the results of Tables 2 to 13, when the oil-soluble licorice extract and the extract of a compound-containing plant having a gallic acid residue were used in combination, the MIC of the oil-soluble licorice extract = 3.13 to 25 μg / mL. It shows a remarkable antibacterial action, and can significantly reduce MIC = 200 to 400 μg / mL in the case of the oil-soluble licorice extract alone. Further, for glabrizine and lycochalcone A, which are purified products of oil-soluble licorice extract, MIC = 12.5 to 25 μg / mL in these cases alone, but extraction of a compound-containing plant having a gallic acid residue When the product was used together, the MIC was 3.13 to 6.25 μg / mL.
Therefore, it was recognized that the oil-soluble licorice extract and the purified product thereof and the extract of the compound-containing plant having a gallic acid residue have a synergistic effect of remarkable antibacterial action.
本発明の抗菌性組成物は、優れた抗菌性と安全性を兼ね備えており、各種飲食品、医薬品、医薬部外品、及び化粧料のいずれかに幅広く用いることができ、これらの保存性を向上させることができる。
The antibacterial composition of the present invention has excellent antibacterial properties and safety, and can be widely used in any of various foods, medicines, quasi-drugs, and cosmetics. Can be improved.
Claims (9)
The antibacterial composition according to any one of claims 1 to 8, which is used in any of foods and drinks, pharmaceuticals, quasi drugs, and cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004228602A JP4798973B2 (en) | 2004-08-04 | 2004-08-04 | Antibacterial composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004228602A JP4798973B2 (en) | 2004-08-04 | 2004-08-04 | Antibacterial composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006045121A true JP2006045121A (en) | 2006-02-16 |
| JP4798973B2 JP4798973B2 (en) | 2011-10-19 |
Family
ID=36024119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004228602A Active JP4798973B2 (en) | 2004-08-04 | 2004-08-04 | Antibacterial composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4798973B2 (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009221148A (en) * | 2008-03-17 | 2009-10-01 | Maruzen Pharmaceut Co Ltd | Immunoactivator and immunoactivation feed for fish |
| JP2012519690A (en) * | 2009-03-04 | 2012-08-30 | メタアクティブ、インコーポレイテッド | Method and material for forming site-activated complex of biomolecule |
| CN103235067A (en) * | 2013-04-23 | 2013-08-07 | 浙江大学 | Method for enriching gallotannin with antioxidation activity from mangos |
| JP2013249263A (en) * | 2012-05-30 | 2013-12-12 | Ajinomoto Co Inc | Gallotannin-containing composition |
| JP2014500038A (en) * | 2010-12-23 | 2014-01-09 | デュポン ニュートリション バイオサイエンシーズ エーピーエス | Bactericidal composition |
| US8809399B1 (en) | 2012-12-23 | 2014-08-19 | Liveleaf, Inc. | Methods of treating gastrointestinal spasms triggered by stress |
| JP2014148495A (en) * | 2013-01-11 | 2014-08-21 | Okayama Univ | Antibacterial agent, and antibacterial activity enhancing agent |
| US9192635B2 (en) | 2011-06-24 | 2015-11-24 | Liveleaf, Inc. | Method of treating damaged mucosal or gastrointestinal tissue by administering a composition comprising a mixture of pomegranate and green tea extracts and releasably bound hydrogen peroxide |
| US9636361B2 (en) | 2007-12-28 | 2017-05-02 | Liveleaf, Inc. | Method of killing a bacteria with a plant-based biocidal solution |
| KR101799984B1 (en) * | 2015-06-01 | 2017-11-21 | 국민대학교산학협력단 | Composition for Preveting Biofilm Formation Containing Extract of licorice |
| US10780072B2 (en) * | 2015-12-01 | 2020-09-22 | The Procter & Gamble Company | Methods for inhibiting conversion of choline to trimethylamine (TMA) |
| JP2021054758A (en) * | 2019-09-30 | 2021-04-08 | 丸善製薬株式会社 | Antibacterial agent and antibacterial skin cosmetics |
| CN113786399A (en) * | 2021-10-13 | 2021-12-14 | 吉林大学 | Application of glabridin in preparation of salmonella III type secretion system inhibitor |
| WO2022030443A1 (en) * | 2020-08-07 | 2022-02-10 | キッコーマン株式会社 | Food/beverage article |
| US11246844B2 (en) | 2016-06-29 | 2022-02-15 | The Procter & Gamble Company | Methods for inhibiting conversion of choline to trimethylamine (TMA) |
| CN115300496A (en) * | 2022-08-04 | 2022-11-08 | 石河子大学 | Catechol nanoparticles, catechol protein nanoparticles, and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6041473A (en) * | 1983-08-15 | 1985-03-05 | Shiraimatsu Shinyaku Kk | Antimicrobial agent |
| JPH09327504A (en) * | 1996-06-11 | 1997-12-22 | Maruzen Pharmaceut Co Ltd | Deodorant |
| JPH11302142A (en) * | 1998-04-24 | 1999-11-02 | Sunstar Inc | Food composition for prevention and treatment of periodontal disease, oral composition and pharmaceutical composition |
| JP2001231523A (en) * | 2000-02-25 | 2001-08-28 | Mitsui Norin Co Ltd | Method of manufacturing food and drink filled and packaged in sterilized condition |
| JP2002223734A (en) * | 2002-01-21 | 2002-08-13 | Mitsui Norin Co Ltd | Low-acid drink containing antifungal agent effective on drug-resistant eumycetes and method for producing the same |
| JP2002234828A (en) * | 2001-02-07 | 2002-08-23 | Nikko Seiyaku Kk | Skin whitening cosmetic preparation |
| WO2003099110A2 (en) * | 2002-05-28 | 2003-12-04 | The Regents Of The University Of California | Herbs and herbal combinations useful for the treatment of microbial infections |
-
2004
- 2004-08-04 JP JP2004228602A patent/JP4798973B2/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6041473A (en) * | 1983-08-15 | 1985-03-05 | Shiraimatsu Shinyaku Kk | Antimicrobial agent |
| JPH09327504A (en) * | 1996-06-11 | 1997-12-22 | Maruzen Pharmaceut Co Ltd | Deodorant |
| JPH11302142A (en) * | 1998-04-24 | 1999-11-02 | Sunstar Inc | Food composition for prevention and treatment of periodontal disease, oral composition and pharmaceutical composition |
| JP2001231523A (en) * | 2000-02-25 | 2001-08-28 | Mitsui Norin Co Ltd | Method of manufacturing food and drink filled and packaged in sterilized condition |
| JP2002234828A (en) * | 2001-02-07 | 2002-08-23 | Nikko Seiyaku Kk | Skin whitening cosmetic preparation |
| JP2002223734A (en) * | 2002-01-21 | 2002-08-13 | Mitsui Norin Co Ltd | Low-acid drink containing antifungal agent effective on drug-resistant eumycetes and method for producing the same |
| WO2003099110A2 (en) * | 2002-05-28 | 2003-12-04 | The Regents Of The University Of California | Herbs and herbal combinations useful for the treatment of microbial infections |
Cited By (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10525080B2 (en) | 2007-12-28 | 2020-01-07 | Liveleaf, Inc. | Increasing the half-life of hydrogen peroxide in an ingestible composition |
| US9636361B2 (en) | 2007-12-28 | 2017-05-02 | Liveleaf, Inc. | Method of killing a bacteria with a plant-based biocidal solution |
| JP2009221148A (en) * | 2008-03-17 | 2009-10-01 | Maruzen Pharmaceut Co Ltd | Immunoactivator and immunoactivation feed for fish |
| JP2012519690A (en) * | 2009-03-04 | 2012-08-30 | メタアクティブ、インコーポレイテッド | Method and material for forming site-activated complex of biomolecule |
| JP2016000743A (en) * | 2009-03-04 | 2016-01-07 | ライブリーフ，インコーポレーテッド | Method and material for site activated complexing of biologic molecules |
| JP2014500038A (en) * | 2010-12-23 | 2014-01-09 | デュポン ニュートリション バイオサイエンシーズ エーピーエス | Bactericidal composition |
| US9192635B2 (en) | 2011-06-24 | 2015-11-24 | Liveleaf, Inc. | Method of treating damaged mucosal or gastrointestinal tissue by administering a composition comprising a mixture of pomegranate and green tea extracts and releasably bound hydrogen peroxide |
| JP2013249263A (en) * | 2012-05-30 | 2013-12-12 | Ajinomoto Co Inc | Gallotannin-containing composition |
| US9603871B2 (en) | 2012-12-23 | 2017-03-28 | Liveleaf, Inc. | Methods of treating gastroesophageal reflux disease |
| US9907818B2 (en) | 2012-12-23 | 2018-03-06 | Liveleaf, Inc. | Methods of treating a treatment-resistant gastrointestinal spasm |
| US9023895B1 (en) | 2012-12-23 | 2015-05-05 | Liveleaf, Inc. | Methods of treating necrotic enteritis |
| US9089596B1 (en) | 2012-12-23 | 2015-07-28 | Liveleaf, Inc. | Methods of treating drug side-effects that include a gastrointestinal spasm |
| US8946304B2 (en) | 2012-12-23 | 2015-02-03 | Liveleaf, Inc. | Methods of treating malnutrition |
| US8822545B1 (en) | 2012-12-23 | 2014-09-02 | Liveleaf, Inc. | Tannin formulation for treating gastrointestinal spasms triggered by stress |
| US9408869B2 (en) | 2012-12-23 | 2016-08-09 | Liveleaf, Inc. | Methods of treating a gastrointestinal spasm associated with chemotherapy or radiation therapy |
| US9603883B2 (en) | 2012-12-23 | 2017-03-28 | Liveleaf, Inc. | Methods of inhibiting a bacterial virulence in a subject |
| US10493102B2 (en) | 2012-12-23 | 2019-12-03 | Liveleaf, Inc. | Methods of inhibiting the virulence of a pathogen with an oxidized tannin to treat a gastrointestinal spasm |
| US8809399B1 (en) | 2012-12-23 | 2014-08-19 | Liveleaf, Inc. | Methods of treating gastrointestinal spasms triggered by stress |
| US10039784B2 (en) | 2012-12-23 | 2018-08-07 | Liveleaf, Inc. | Methods of treating a treatment-resistant gastrointestinal spasm with an oxidized tannin |
| US8952072B2 (en) | 2012-12-23 | 2015-02-10 | Liveleaf, Inc. | Tannin formulation for treating malnutrition |
| JP2014148495A (en) * | 2013-01-11 | 2014-08-21 | Okayama Univ | Antibacterial agent, and antibacterial activity enhancing agent |
| CN103235067A (en) * | 2013-04-23 | 2013-08-07 | 浙江大学 | Method for enriching gallotannin with antioxidation activity from mangos |
| KR101799984B1 (en) * | 2015-06-01 | 2017-11-21 | 국민대학교산학협력단 | Composition for Preveting Biofilm Formation Containing Extract of licorice |
| US10780072B2 (en) * | 2015-12-01 | 2020-09-22 | The Procter & Gamble Company | Methods for inhibiting conversion of choline to trimethylamine (TMA) |
| US10786479B2 (en) | 2015-12-01 | 2020-09-29 | The Procter & Gamble Company | Methods for inhibiting conversion of carnitine to trimethylamine (TMA) |
| US11246844B2 (en) | 2016-06-29 | 2022-02-15 | The Procter & Gamble Company | Methods for inhibiting conversion of choline to trimethylamine (TMA) |
| JP2021054758A (en) * | 2019-09-30 | 2021-04-08 | 丸善製薬株式会社 | Antibacterial agent and antibacterial skin cosmetics |
| JP7485325B2 (en) | 2019-09-30 | 2024-05-16 | 丸善製薬株式会社 | Antibacterial agents and antibacterial skin cosmetics |
| WO2022030443A1 (en) * | 2020-08-07 | 2022-02-10 | キッコーマン株式会社 | Food/beverage article |
| CN113786399A (en) * | 2021-10-13 | 2021-12-14 | 吉林大学 | Application of glabridin in preparation of salmonella III type secretion system inhibitor |
| CN115300496A (en) * | 2022-08-04 | 2022-11-08 | 石河子大学 | Catechol nanoparticles, catechol protein nanoparticles, and preparation method and application thereof |
| CN115300496B (en) * | 2022-08-04 | 2023-09-26 | 石河子大学 | Catechol nanoparticle, catechol protein nanoparticle, and preparation methods and applications thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4798973B2 (en) | 2011-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4798973B2 (en) | Antibacterial composition | |
| JP2023547872A (en) | Novel Lactobacillus strains and their uses | |
| JP5226297B2 (en) | Method for producing at least one of 8-hydroxyliquiritigenin and 3'-hydroxyisoquiritigenin | |
| JP2023052866A (en) | Hair restorer, cosmetic, food and drink | |
| KR102086166B1 (en) | Antimicrobial composition comprising the extracts of rambutan peel | |
| JP2022001589A (en) | Anti-obesity agent, cosmetic product, and food and drink products | |
| KR101602552B1 (en) | Antimicrobial composition comprising the extract of cornus officinalis | |
| JP2007282632A (en) | Method for preparation of dried plant extract | |
| JP2003055244A (en) | Agent promoting hyaluronic acid production, cosmetics, food and beverage including the hyaluronic acid production-promoting agent | |
| KR101718611B1 (en) | Novel microorganism producing carotinoid | |
| KR100876667B1 (en) | The complex extract of Myristica fra grans Houtt, Cinnamomum cassia Blume and Alpinia officinarum Hance, antibacterial activity fraction isolated, preparation method and uses thereof | |
| JP5095893B2 (en) | Active oxygen scavenger | |
| JP5832714B2 (en) | Tyrosinase activity inhibitor and melanin production inhibitor | |
| JP2005343823A (en) | Antibacterial composition | |
| KR101636608B1 (en) | Composition for antioxidation comprising the seed extract of cornus officinalis | |
| KR20140106058A (en) | Composition for enhancing immunological activity comprising soluble salmon milt extract and vegetable lactic acid bacteria | |
| KR20180107785A (en) | Antimicrobial composition comprising organic solvent fractions of arctium lappa root extracts as an effective component | |
| JP2012006905A (en) | Composition for skin care | |
| KR101685671B1 (en) | Pharmaceutical composition for preventing or treating peridontal disease comprising extract of Lignum Acronychiae containing nerolidol | |
| JP4427233B2 (en) | Antibacterial composition | |
| JP5422137B2 (en) | Whitening agent, tyrosinase activity inhibitor, melanin production inhibitor, and skin external preparation for whitening | |
| JP2017165686A (en) | Liver function improver | |
| JP2010202606A (en) | Aggregation inhibitor of amyloid-forming protein and decomposition agent of aggregated amyloid protein | |
| JP3710854B2 (en) | Easily water soluble bayberry plant extract | |
| KR102323665B1 (en) | Antibacterial and synergic effect of diphlorethohydroxycarmalol derived from brown alga Ishige okamurae against Pseudomonas aeruginosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070619 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20101026 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20101227 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110125 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110316 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20110405 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110602 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20110623 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20110719 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20110802 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140812 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 4798973 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |