JP2006042794A5 - - Google Patents

Download PDF

Info

Publication number
JP2006042794A5
JP2006042794A5 JP2004354966A JP2004354966A JP2006042794A5 JP 2006042794 A5 JP2006042794 A5 JP 2006042794A5 JP 2004354966 A JP2004354966 A JP 2004354966A JP 2004354966 A JP2004354966 A JP 2004354966A JP 2006042794 A5 JP2006042794 A5 JP 2006042794A5
Authority
JP
Japan
Prior art keywords
resin
fine particles
specific gravity
base polymer
resin beads
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2004354966A
Other languages
Japanese (ja)
Other versions
JP2006042794A (en
Filing date
Publication date
Application filed filed Critical
Priority to JP2004354966A priority Critical patent/JP2006042794A/en
Priority claimed from JP2004354966A external-priority patent/JP2006042794A/en
Publication of JP2006042794A publication Critical patent/JP2006042794A/en
Publication of JP2006042794A5 publication Critical patent/JP2006042794A5/ja
Pending legal-status Critical Current

Links

Claims (11)

ベースポリマー（ａ）と、（ａ）の真比重より小さい見掛け比重を持つ微粒子（ｂ）とを含有してなることを特徴とする細胞培養用樹脂ビーズ。 A resin bead for cell culture comprising a base polymer (a) and fine particles (b) having an apparent specific gravity smaller than the true specific gravity of (a). ベースポリマー（ａ）の真比重（Ｄａ）が１．０５０〜１．６０である請求項１に記載の樹脂ビーズ。 The resin beads according to claim 1, wherein the base polymer (a) has a true specific gravity (Da) of 1.050 to 1.60. 微粒子（ｂ）の見掛け比重（Ｄｂ）が０．０３〜０．９８である請求項１又は２に記載の樹脂ビーズ。 The resin beads according to claim 1 or 2, wherein the apparent specific gravity (Db) of the fine particles (b) is 0.03 to 0.98. ベースポリマー（ａ）の真比重（Ｄａ）と微粒子（ｂ）の見掛け比重（Ｄｂ）との差（Ｄａ−Ｄｂ）が０．０１〜１．５８である請求項１〜３のいずれかに記載の樹脂ビーズ。 The difference (Da-Db) between the true specific gravity (Da) of the base polymer (a) and the apparent specific gravity (Db) of the fine particles (b) is 0.01 to 1.58. Resin beads. 樹脂ビーズ中の微粒子（ｂ）の含有量が樹脂ビーズの重量に基づいて０．０１〜５０重量％である請求項１〜４のいずれかに記載の樹脂ビーズ。 The resin beads according to any one of claims 1 to 4, wherein the content of the fine particles (b) in the resin beads is 0.01 to 50% by weight based on the weight of the resin beads. 見掛け比重が１．０１〜１．０５である請求項１〜５のいずれかに記載の樹脂ビーズ。 The apparent specific gravity is 1.01-1.05, The resin bead in any one of Claims 1-5. ベースポリマー（ａ）が架橋ウレタン樹脂、架橋エポキシ樹脂、架橋ビニル樹脂及び架橋エステル樹脂からなる群より選ばれる少なくとも１種である請求項１〜６のいずれかに記載の樹脂ビーズ。 The resin beads according to any one of claims 1 to 6, wherein the base polymer (a) is at least one selected from the group consisting of a crosslinked urethane resin, a crosslinked epoxy resin, a crosslinked vinyl resin, and a crosslinked ester resin. 細胞接着性最小アミノ酸配列（Ｘ）を１分子中に少なくとも１個有するポリペプチド（Ｐ）を樹脂ビーズの表面に含有してなる請求項１〜７のいずれかに記載の樹脂ビーズ。 The resin bead according to any one of claims 1 to 7, comprising a polypeptide (P) having at least one cell adhesion minimal amino acid sequence (X) per molecule on the surface of the resin bead. 請求項１〜８のいずれかに記載の樹脂ビーズ及び無血清培地を用いて、細胞培養する工程を含むことを特徴とする細胞の生産方法。 A method for producing cells comprising the step of culturing cells using the resin beads and serum-free medium according to any one of claims 1 to 8. 請求項１〜８のいずれかに記載の樹脂ビーズを用いて、幹細胞を細胞培養する工程を含むことを特徴とする細胞の生産方法。 A method for producing cells comprising the step of culturing stem cells using the resin beads according to claim 1. 下記（１）〜（４）のいずれかの方法であることを特徴とする、ベースポリマー（ａ）と、（ａ）の真比重より小さい見掛け比重を持つ微粒子（ｂ）とを含有してなる細胞培養用樹脂ビーズの製造方法。
（１）ベースポリマー（ａ）と微粒子（ｂ）との液状混合物を水性媒体に乳化分散する方法。
（２）ベースポリマー（ａ）と微粒子（ｂ）との液状混合物を噴霧する方法。
（３）ベースポリマー（ａ）と微粒子（ｂ）との固状混合物を破砕又は粉砕する方法。
（４）ベースポリマー（ａ）の前駆体と微粒子（ｂ）との液状混合物を乳化分散しながら、または乳化分散してから重合する方法。
The base polymer (a) is characterized by being one of the following methods (1) to (4), and contains fine particles (b) having an apparent specific gravity smaller than the true specific gravity of (a). A method for producing resin beads for cell culture.
(1) A method of emulsifying and dispersing a liquid mixture of a base polymer (a) and fine particles (b) in an aqueous medium.
(2) A method of spraying a liquid mixture of the base polymer (a) and the fine particles (b).
(3) A method of crushing or pulverizing the solid mixture of the base polymer (a) and the fine particles (b).
(4) A method in which a liquid mixture of the precursor of the base polymer (a) and the fine particles (b) is polymerized while being emulsified and dispersed or after being emulsified and dispersed.
JP2004354966A 2004-06-28 2004-12-08 Resin bead for cell culture Pending JP2006042794A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2004354966A JP2006042794A (en) 2004-06-28 2004-12-08 Resin bead for cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004190285 2004-06-28
JP2004354966A JP2006042794A (en) 2004-06-28 2004-12-08 Resin bead for cell culture

Publications (2)

Publication Number Publication Date
JP2006042794A JP2006042794A (en) 2006-02-16
JP2006042794A5 true JP2006042794A5 (en) 2007-09-20

Family

ID=36022034

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2004354966A Pending JP2006042794A (en) 2004-06-28 2004-12-08 Resin bead for cell culture

Country Status (1)

Country Link
JP (1) JP2006042794A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8563312B2 (en) 2008-01-30 2013-10-22 Geron Corporation Synthetic surfaces for culturing stem cell derived cardiomyocytes

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329469B2 (en) 2008-01-30 2012-12-11 Geron Corporation Swellable (meth)acrylate surfaces for culturing cells in chemically defined media
DK2247716T3 (en) 2008-01-30 2012-05-29 Geron Corp Synthetic surfaces for growing cells in chemically defined media
SG188096A1 (en) 2008-01-30 2013-03-28 Geron Corp Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells
EP3971201A4 (en) * 2019-05-15 2023-11-22 Sekisui Chemical Co., Ltd. Cell culturing scaffold material and cell culturing container

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0775711B2 (en) * 1986-06-28 1995-08-16 清水建設株式会社 Floating granules and method for producing the same
JP2003189848A (en) * 2001-10-19 2003-07-08 Sanyo Chem Ind Ltd Bead for culturing animal cell
JP2003304868A (en) * 2002-04-17 2003-10-28 Sanyo Chem Ind Ltd Polypeptide-containing substrate

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8563312B2 (en) 2008-01-30 2013-10-22 Geron Corporation Synthetic surfaces for culturing stem cell derived cardiomyocytes

Similar Documents

Publication Publication Date Title
KR101099058B1 (en) Method for producing polymer beads of uniform size
Miletić et al. Macroporous poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins—Versatile immobilization supports for biocatalysts
US9279102B2 (en) Temperature-responsive substrate for cell culture and production method thereof
JP5222116B2 (en) Method for producing thermally expandable microcapsules
EP0696610A3 (en) Acrylic sheet, acrylic adhesive sheet and process for preparing the sheets
JP2009535025A5 (en)
EP1602670B8 (en) Production method of porous spherical particles of vinyl polymer
JP2008104359A (en) Carrier for immobilizing enzyme, immobilized enzyme and method for producing carrier for immobilizing enzyme
JP2006042794A5 (en)
Kaul et al. Stereoselective nitrile hydrolysis by immobilized whole-cell biocatalyst
CN105037626A (en) Method for preparing big hole large particle diameter polymer microspheres
CN109486342A (en) A kind of anticorrosive paint of D structure graphene reinforced epoxy
WO2004056976A3 (en) Process for coating cell-culture support
CN106565908B (en) A kind of preparation method of monodispersed large grain-size polymer microballoon
JP2008208368A (en) Monodispersed weak acidic cation exchanger
US20150064468A1 (en) Product and method for making uniform spherical, acrylic polymeric beads
JP2005027532A (en) Cell culture base material and method for producing the same, and method for culturing cell
JP2007217616A (en) Deformed polymer particulate and method for producing the same
CN206512227U (en) A kind of carrier for being used to expand multipotential stem cell
JP2001329209A5 (en)
JP2005326597A5 (en)
CN104603160B (en) Mixed salt suspension polymerization process and resins and catalysts produced thereof
JP3775706B2 (en) Method for producing granular molded product for enzyme or microbial cell immobilization
Divya et al. Catalytic amidolysis of amino acid p-nitroanilides using transition state analogue imprinted artificial enzymes: Cooperative effect of pyridine moiety
JP2005534753A5 (en)