JP2005503762A5 - - Google Patents
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- Publication number
- JP2005503762A5 JP2005503762A5 JP2002561074A JP2002561074A JP2005503762A5 JP 2005503762 A5 JP2005503762 A5 JP 2005503762A5 JP 2002561074 A JP2002561074 A JP 2002561074A JP 2002561074 A JP2002561074 A JP 2002561074A JP 2005503762 A5 JP2005503762 A5 JP 2005503762A5
- Authority
- JP
- Japan
- Prior art keywords
- hybridization buffer
- molecular weight
- dextran sulfate
- daltons
- vol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 6
- 229960000633 Dextran Sulfate Drugs 0.000 claims 3
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 2
- 210000001519 tissues Anatomy 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 1
- 238000001704 evaporation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000010186 staining Methods 0.000 claims 1
Claims (4)
- スライド上のハイブリダイゼーション緩衝液被覆組織を覆っている蒸発防止液体を有する自動化組織染色系に用いるためのハイブリダイゼーション緩衝液であって、改良点が、約８，０００から約１６，０００ダルトンまでの範囲の分子量を有する低分子量デキストラン硫酸を含む、上記ハイブリダイゼーション緩衝液。
- 前記デキストラン硫酸が約１３，０００ダルトンの平均分子量を有する、請求項１記載のハイブリダイゼーション緩衝液。
- 低分子量デキストラン硫酸の前記濃度が、ｗｔ．／ｖｏｌ．で約５％から約２５％までの範囲にわたる、請求項１記載のハイブリダイゼーション緩衝液。
- 前記緩衝液が、ｗｔ．／ｖｏｌ．で約５％から約８０％までの濃度を有するホルムアミドを場合によっては含有する、請求項１記載のハイブリダイゼーション緩衝液。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/772,123 US6656685B2 (en) | 2001-01-29 | 2001-01-29 | Hybridization buffers using low molecular weight dextran sulfate and methods for their use |
| PCT/US2002/002642 WO2002061139A2 (en) | 2001-01-29 | 2002-01-29 | Hybridization buffers using low molecular weight dextran sulfate and methods for their use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005503762A JP2005503762A (ja) | 2005-02-10 |
| JP2005503762A5 true JP2005503762A5 (ja) | 2005-12-22 |
Family
ID=25093987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002561074A Pending JP2005503762A (ja) | 2001-01-29 | 2002-01-29 | 低分子量デキストラン硫酸を使用するハイブリダイゼーション緩衝液およびそれらの使用方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6656685B2 (ja) |
| EP (1) | EP1356116B1 (ja) |
| JP (1) | JP2005503762A (ja) |
| AT (1) | ATE361995T1 (ja) |
| AU (1) | AU2002240180B2 (ja) |
| CA (1) | CA2434854A1 (ja) |
| DE (1) | DE60220018T2 (ja) |
| WO (1) | WO2002061139A2 (ja) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002305300B2 (en) * | 2001-04-30 | 2006-06-01 | Ventana Medical Systems, Inc. | Reagents and methods for automated in situ or microarray hybridization |
| WO2005033333A2 (en) | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
| DE102004028303A1 (de) * | 2004-06-11 | 2005-12-29 | Roche Diagnostics Gmbh | Verfahren und Vorrichtung zum Nachweis von Analyten |
| EP2057289B1 (en) * | 2006-08-09 | 2016-08-10 | Univ Singapore | METHOD FOR MOLECULAR BIOLOGY APPLICATIONS |
| AU2007322261B2 (en) | 2006-11-01 | 2014-01-23 | Ventana Medical Systems, Inc. | Haptens, hapten conjugates, compositions thereof and method for their preparation and use |
| CA2687178C (en) * | 2007-05-23 | 2014-02-04 | Ventana Medical Systems, Inc. | Polymeric carriers for immunohistochemistry and in situ hybridization |
| EP2285982B1 (en) | 2008-05-27 | 2018-04-11 | Dako Denmark A/S | Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers |
| ES2906426T3 (es) | 2008-06-05 | 2022-04-18 | Ventana Med Syst Inc | Composición para procesamiento histoquímico |
| WO2010097655A1 (en) | 2009-02-26 | 2010-09-02 | Dako Denmark A/S | Compositions and methods for rna hybridization applications |
| WO2011057017A1 (en) * | 2009-11-06 | 2011-05-12 | Bend Research, Inc. | Aqueous nanoparticle suspensions for use in drug discovery |
| WO2012125123A1 (en) | 2011-03-11 | 2012-09-20 | National University Of Singapore | Pericyte progenitors from peripheral blood |
| US10662465B2 (en) * | 2011-09-30 | 2020-05-26 | Agilent Technologies, Inc. | Hybridization compositions and methods using formamide |
| EP2768974B1 (en) | 2011-10-21 | 2017-07-19 | Dako Denmark A/S | Hybridization compositions and methods |
| US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
| DK3133166T4 (da) * | 2015-08-21 | 2023-03-20 | 42 Life Sciences Gmbh & Co Kg | Sammensætning og fremgangsmåde til hybridisering |
| EP3351263A1 (en) | 2017-01-20 | 2018-07-25 | Universitätsklinikum Hamburg-Eppendorf | Pharmaceutical preparation for treating or preventing tissue adhesion |
| BR112020003403A2 (pt) | 2017-08-18 | 2020-08-25 | Neutrolis Inc. | enzimas de dnase engenheiradas e uso na terapia |
| MX2021001709A (es) * | 2018-08-15 | 2021-05-27 | Illumina Inc | Composiciones y metodos para mejorar el enriquecimiento de genotecas. |
| US11058724B2 (en) | 2018-10-08 | 2021-07-13 | Neutrolis, Inc. | Methods of using DNASE1-like 3 in therapy |
| US10988746B2 (en) | 2018-10-08 | 2021-04-27 | Neutrolis, Inc. | Manufacturing and engineering of DNASE enzymes for therapy |
| BR112021006737A2 (pt) * | 2018-10-08 | 2021-07-13 | Neutrolis, Inc. | engenharia de enzimas dnase para fabricação e terapia |
| US11352613B2 (en) | 2019-02-04 | 2022-06-07 | Neutrolis, Inc. | Engineered human extracellular DNASE enzymes |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4302204A (en) * | 1979-07-02 | 1981-11-24 | The Board Of Trustees Of Leland Stanford Junior University | Transfer and detection of nucleic acids |
| US4689294A (en) | 1984-11-19 | 1987-08-25 | Miles Laboratories, Inc. | Enhancement of hybridization of nucleic acids by anionic polymers |
| US5985549A (en) | 1985-10-22 | 1999-11-16 | University Of Massachusetts | Non-isotopic in-situ hybridization method for detection of nucleic acids |
| US5447841A (en) * | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| US5273905A (en) * | 1991-02-22 | 1993-12-28 | Amoco Corporation | Processing of slide mounted material |
| US5750340A (en) | 1995-04-07 | 1998-05-12 | University Of New Mexico | In situ hybridization solution and process |
| US6127188A (en) * | 1995-10-06 | 2000-10-03 | Mj Research, Inc. | Method and apparatus for controlling evaporation in histological procedures |
| US5763185A (en) * | 1996-12-30 | 1998-06-09 | Becton Dickinson And Company | Method for reducing inhibitors of nucleic acid hybridization |
| ATE489613T1 (de) * | 1998-02-27 | 2010-12-15 | Ventana Med Syst Inc | Automatisierter molekularer pathologieapparat mit unabhängigen objektträgerwärmern |
| US6855552B2 (en) * | 1998-09-03 | 2005-02-15 | Ventana Medical Systems | Automated immunohistochemical and in situ hybridization assay formulations |
-
2001
- 2001-01-29 US US09/772,123 patent/US6656685B2/en not_active Expired - Fee Related
-
2002
- 2002-01-29 AT AT02706073T patent/ATE361995T1/de not_active IP Right Cessation
- 2002-01-29 JP JP2002561074A patent/JP2005503762A/ja active Pending
- 2002-01-29 DE DE60220018T patent/DE60220018T2/de not_active Expired - Fee Related
- 2002-01-29 CA CA002434854A patent/CA2434854A1/en not_active Abandoned
- 2002-01-29 AU AU2002240180A patent/AU2002240180B2/en not_active Ceased
- 2002-01-29 WO PCT/US2002/002642 patent/WO2002061139A2/en active IP Right Grant
- 2002-01-29 EP EP02706073A patent/EP1356116B1/en not_active Expired - Lifetime
-
2003
- 2003-08-28 US US10/652,676 patent/US20040253603A1/en not_active Abandoned
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