JP2005343796A - Cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To prepare a cosmetic free from adverse effect on the skin, or the like and having also high safety in addition to unconventional high bleaching effect. <P>SOLUTION: The present invention enabled development of the cosmetic free from adverse effect on the skin, or the like and having also high safety in addition to unconventional high bleaching effect by synergistic action obtained by combination of a protease capable of degrading a horny layer protein with a peroxidase having a melanin-decomposing property. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

The present invention relates to a whitening cosmetic, more specifically, by incorporating protease and a melanin-degrading substance, particularly manganese peroxidase, melanin present in the epidermal horny layer can be efficiently decomposed and removed. The present invention relates to a whitening cosmetic having an unprecedented high effect.

In addition to the skin, melanin is biosynthesized in the central nerve and retina. In addition to animals, it is widely distributed in nature from plants to microorganisms. Melanin is produced by the conversion of tyrosine to dopa and further dopaquinone by the action of tyrosinase, further oxidation to indole-5,6-dihydroquinone, which is polymerized.

The biosynthesis of melanin takes place in melanocytes present in the basal layer of the epidermis. In melanocytes, melanin is synthesized and matured in granules called melanosomes, migrates and disperses into epidermal cells, and finally becomes plaque and falls off. Melanin plays an important role in protecting the body from the harmful effects of ultraviolet rays and is an important medical factor. However, if the amount of melanin is too large, it becomes dark skin, which is a serious cosmetic problem such as rash, stain, buckwheat.

Currently known countermeasures against Kusumi, stains and buckwheat include a method of shielding the ultraviolet rays that trigger melanin biosynthesis with sunscreen agents, melanin synthesis inhibitors such as arbutin, kojic acid, vitamin C derivatives, and melanin biosynthesis. A method using a substance that inhibits biosynthesis of melanin such as a plant extract that suppresses synthesis is known.

However, the present condition is that the safe method without the side effect for decomposing | disassembling and disappearing the melanin in the stratum corneum once formed has not been developed yet. In the past, hydroquinone, 4-isopropylcatechol, and hydroquinone monobenzyl ether have been used as skin bleaching agents, but these substances have a strong decoloring action, but their action is based on degeneration and lethality of pigmented cells. Therefore, when it was continuously applied externally, not only became permanent vitiligo, but also the side effects of skin troubles such as rash were unavoidable. For this reason, these agents are not currently marketed, and there has been a strong demand for the development of a whitening agent that is safe and effective against Kusumi, blemishes and buckwheat.

Under these circumstances, a method for decomposing stratum corneum melanin with peroxidase has been proposed as a measure for removing rashes. However, peroxidase alone does not provide a sufficient melanolytic action. Cosmetics using protease have also been used for a long time, but have a stratum corneum protein removal effect, but are not effective in decomposing / removing melanin itself, and have a sufficient effect on Kusumi, stains, and freckles There was no.

JP-A-6-219933 Japanese Patent Laid-Open No. 7-213294 JP-A-8-217659

The present invention has been made in view of the state of the art as described above, and develops a cosmetic that has high safety and no side effects on the skin in addition to having an unprecedented high whitening effect. It was made for the purpose.

In order to achieve the above-mentioned purpose, a combination of a protease capable of degrading stratum corneum protein and a peroxidase having melanin decomposing ability, it has a synergistic effect, and it has never had a whitening effect on Kumi, blemishes and buckwheat. In addition, the present invention has been found to have high safety with respect to the skin.

The protease that can be used in the present invention is not particularly limited, and any microorganism-derived, plant-derived, or animal-derived protease can be used. Examples of microorganism-derived proteases include mold-derived proteases, yeast-derived proteases, and bacterial-derived proteases. Examples of plant-derived proteases include papain, chymopapain, promeline, ficin and the like. Examples of animal-derived proteases include pepsin, trypsin, chymotrypsin, cathepsin, pancreatin and the like.

As a peroxidase that can be used in the present invention, a plant-derived peroxidase includes, for example, horseradish peroxidase as a representative example, but is not particularly limited. Examples of animal-derived substances include lactoperoxidase and myeloperoxidase, but are not particularly limited. Examples of microorganism-derived substances include laccase, lignin peroxidase, and manganese peroxidase, but are not particularly limited.

As the protease and peroxidase that can be used in the present invention, any of the enzymes described above can be used. Among these enzymes, many proteases are commercially available, and any of them can be suitably used. For example, Nagase Seikagaku biopulase, purified papain and the like can be exemplified. As for peroxidase, commercially available enzymes can be used, but they can also be produced by culturing microorganisms.

Among peroxidases, manganese peroxidase, in particular, can be suitably produced by culturing mycelium of basidiomycetes. Basidiomycetes can induce mycelia from natural fruiting bodies and soil, and can also be sold from strain distribution agencies. By culturing these mycelia, it is possible to produce a melanin-degrading substance such as manganese peroxidase in the culture solution.

In the present invention, the amount of each enzyme obtained by any of the methods described above is not particularly limited, but the enzyme activity value during use is 1 to 1000 units / ml, preferably 20 to 100 units / ml. It is desirable to prepare it. Moreover, in order to prepare so that it may become said enzyme activity value, 0.01-10 weight% of enzyme powder whose enzyme activity value is 1,000-100,000 unit / g, Preferably it is 0.2-1 weight. It is desirable to mix so that it may become%. In addition, although the compounding ratio of the enzyme of protease and peroxidase is not specifically limited, The ratio of peroxidase 0.01-100 with respect to protease 1 can be used by a titer activity ratio. Furthermore, the ratio of 0.1-10 is suitable.

In addition to the above essential components, the cosmetics of the present invention are aqueous components, oily components, plant extracts, animal extracts, powders, excipients, surfactants, oils, alcohols, pH adjusters, preservatives, antioxidants. It is prepared by mixing an agent, a thickener, a pigment, a fragrance, and the like as necessary and blending appropriately. The dosage form of the cosmetic of the present invention is not particularly limited, and can be various dosage forms such as lotion, milky lotion, cream, pack, powder, spray, ointment, dispersion, and cleaning agent.

The cosmetic of the present invention enhances the effect of decomposing melanin in the keratin due to the synergistic effect of protease and peroxidase, and has high whitening effect against kusumi, blemishes and buckwheat, as well as high safety against skin. Also has sex.

In the following, examples relating to the elimination of kusumumi, blemishes and freckles due to the melanin decomposing effect of various products according to the present invention will be shown and examples of prescriptions applied to cosmetics using the materials will be described, but the present invention was described here Needless to say, the present invention is not limited to the examples.

Preparation of Manganese Peroxidase In order to prepare manganese peroxidase, culturing was performed using oyster mushroom (IFO: No. 30776) strain purchased from the Fermentation Research Institute. The medium used was a sterilized medium in which 300 g of yeast extract, 300 g of polypeptone, 300 g of malt extract, and 2000 g of sucrose were dissolved in 100 L of purified water. The culture was performed by adding 100 ml of the culture solution to a 200 ml Erlenmeyer flask, sterilizing, planting the mycelium, planting the rotating culture at 24 ° C. and 150 rpm for 10 days as the inoculum, and performing aeration culture at 24 ° C. for 14 days. After completion of the culture, the mycelium was removed to obtain a culture solution. After concentration, dextrin was added and spray-dried to make powder.

Determination of manganese peroxidase titer
Add 1 ml of 2,6-dimethoxyphenol (2,6-DMP) 1% aqueous solution, 1.0 ml of buffer solution prepared to pH 4.5 with 0.2 M-citric acid-NaOH, and 1.0 ml of purified water and mix. 1 g of the above culture solution powder is dissolved in 10 ml of purified water, and 1 ml of the solution is added to the mixture and left at 37 ° C. Absorbance at 475 nm was measured before the addition of the culture solution powder solution and 10 minutes after the addition.
Manganese peroxidase has a titer of 475nm in a 1% aqueous solution of 2,6-dimethoxyphenol (2,6-DMP).
The concentration at which the absorbance value was increased by 1 per minute was 1 unit / ml. The result of measuring the titer of the culture powder was 2,000 units / ml.

As panelists for confirming the effects of panelists, 30 women aged 20 to 50 years were divided into three groups: A, B, and C. Each group of panelists received samples twice a day (morning and evening) for two consecutive months In the meantime, each of the product of the present invention and the comparative product was used, and the state of the application site was compared before and after the test to confirm the improvement effect. The sample used for this test prepared (1) lotion shown in [0024] and (2) emulsion shown in [0025] as examples. As Comparative Example 1, a lotion and a milky lotion were prepared by removing the manganese peroxidase powder from (1) the lotion shown in [0024] and (2) the milky lotion shown in [0025]. In Comparative Example 2, (1) lotion shown in [0024], and (2) lotion and emulsion obtained by removing the biolase powder from the emulsion shown in [0025] were prepared. The group A panelists used the skin lotion and emulsion of the example formulation in the first half of the month, and the skin lotion and emulsion of the comparative example 1 in the second half of the month, and confirmed the improvement effect. . Group B panelists used the skin lotion and emulsion of Comparative Example 1 in the first half of the month, and used the skin lotion and milk of the Example Formula in the latter half of the month, and confirmed the improvement effect. Group C panelists were allowed to use the skin lotion and emulsion of Comparative Example 2 in the first half of the month, and the skin lotion and emulsion of Example Formula in the latter half of the month, and confirmed the improvement effect. The panelists of each group were answered by questionnaire before starting the test and after using it for 2 months. The results are shown in Table 1.

As is clear from Table 1, compared with Comparative Example 1 consisting only of the conventional protease and Comparative Example 2 consisting only of the peroxidase, the Example consisting of the protease and peroxidase of the present invention is highly effective. It was. A questionnaire regarding skin irritation was also conducted at the same time, but no panelists felt irritation including mild discomfort to the skin throughout the test period and at the end of the test using the examples for one month. In addition, panelists who used Comparative Example 1 and Comparative Example 2 for 1 month and felt skin irritation during and after the test period were zero, but there were some panelists who felt uncomfortable. From this result, it is clear that the present invention has high safety.

Next, although the example of the formulation example of the cosmetics which mix | blended various components of this invention is shown, this invention is not limited to this.

Cosmetic formulation example (1) lotion
(weight%)
a) Bioplase powder (10,000unit / g) 1.0
b) Mangan oxidase powder (10,000unit / g) 1.0
c) Dipotassium glycyrrhizinate
0.1
d) Glycerin
5.0
e) Hyaluronic acid 0.05
f) Polyoxyethylene sorbitan monolaurate (20E.O.)
1.0
g) ethanol
6.0
h) Fragrance
Appropriate amount
i) Preservatives and antioxidants
Appropriate amount
j) Purified water
The remaining preparation methods a) to e) and j) are uniformly mixed to obtain an aqueous phase. f) to i) are mixed and dissolved uniformly, and mixed with the aqueous phase for use.

(2) Latex
(weight%)
a) Beeswax
0.5
b) Petrolatum
2.0
c) Squalane
8.0
d) Sorbitan sesquioleate
0.8
e) Polyoxyethylene oleyl ether (20E.O.) 1.2
f) Gray shimeji broth
15.0
g) Ireisen extract
2.0
h) 1,3-butylene glycol
7.0
i) Carboxyvinyl polymer
0.2
j) Potassium hydroxide
0.1
k) Purified water
The rest
l) Preservatives and antioxidants
Appropriate amount
m) ethanol
7.0
n) Biolase powder (1,000unit / g) 0.2
0) Manganese oxidase powder (1,000unit / g) 0.2
The production methods a) to e) are heated and dissolved to obtain an oil phase, which is kept at 80 ° C. Heat up to h) to l), keep in water phase at 80 ° C., add to a) to e), emulsify, and cool to 50 ° C. with stirring. Add f), g), m) at 50 ° C., stir to 40 ° C. and cool. When using, dissolve n) and 0) in the emulsion prepared by the above-mentioned method.

(3) Lotion
(weight%)
a) Refined papain powder (10,000unit / g) 0.01
b) Mangan oxidase powder (10,000unit / g) 0.01
c) Dipotassium glycyrrhizinate
0.1
d) Glycerin
5.0
e) Hyaluronic acid 0.05
f) Polyoxyethylene sorbitan monolaurate (20E.O.) 1.0
g) ethanol
6.0
h) Fragrance
Appropriate amount
i) Preservatives and antioxidants
Appropriate amount
j) Purified water
The remaining preparation methods a) to e) and j) are uniformly mixed to obtain an aqueous phase. f) to i) are mixed, dissolved uniformly, and mixed with the aqueous phase for use.

(5) Powder face cleanser
(weight%)
a) Manganese oxidase powder (1,000unit / g)
10.0
b) Biolase powder (1,000unit / g)
10.0
c) Peonies extract 0.5
d) Talc
The rest
e) Cellulose
20.0
f) Potassium myristate
30.0
g) Sodium lauryl phosphate
10.0
h) Fragrance
Appropriate amount
i) Preservatives
Appropriate amount of production method a) to i) are mixed and stirred and dispersed well to make uniform. Dissolve in water when used and foam.

(5) Sheet pack
(weight%)
a) Manganese oxidase powder (10,000unit / g)
0.75
b) Purified phine powder (10,000unit / g)
0.5
c) Trimethylglycine
2.0
d) Glycerin
5.0
e) Hyaluronic acid 0.05
f) Purified water
Mix the rest of the production methods a) to f) and stir and disperse them uniformly. A face mask cut into a face mold is impregnated with an appropriate amount and applied to the face.

In addition to having a high whitening effect that has never been seen before, the present invention has no side effects on the skin and the like, and has high safety. Therefore, the present invention can be widely used in cosmetics and pharmaceuticals.

Claims (3)

A cosmetic comprising a protease and a melanin degradable substance. The cosmetic according to claim 1, wherein the melanin-degrading substance is peroxidase. 3. The cosmetic according to claim 1, wherein the melanin degrading substance is manganese peroxidase.