JP2005330270A - Decalcification inhibitory composition and food and beverage containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an oral cavity composition exhibiting a decalcification inhibitory activity under an acidic condition, and to provide a food and beverage containing the same. <P>SOLUTION: The decalcification inhibitory composition contains at least 1 kind selected from maltodextrin phosphate, a reduced maltodextrin phosphate, an oligo-saccharide phosphate, a starch phosphate and their salts, and the food, the beverage, a seasoning, an oral cavity preparation, a medicine, a cosmetic, feed or a fertilizer containing the decalcification inhibitory composition are provided. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a composition for inhibiting demineralization that suppresses demineralization under the surface of the tooth surface under acidic conditions, food and drink containing the same, and the like.

  Although the oral cavity is exposed to various environments, it usually repeats demineralization (dissolution of hydroxyapatite under the tooth surface) and remineralization (calcium phosphate recrystallizes under the tooth surface). When this balance is lost, remineralization cannot catch up with decalcification, and when self-repair is impossible, initial caries (subsurface demineralization) occurs. The first step in preventing caries is to prevent this subsurface demineralization.

  Demineralization is said to occur when the oral pH drops to about 5.5 or lower. This is mainly due to (1) monosaccharides and oligosaccharides such as sucrose and glucose, which are mainly fermentable carbohydrates, or these The reason is that the pH is lowered by the acid produced by oral bacteria using the polymer of (2), and (2) the acidic food or drink is in direct contact with the tooth surface. As a method for preventing such demineralization, xylitol and fluorine are known to be effective. In addition, Patent Document 1 discloses prevention of caries and suppression of progression using hexafluorotitanate as an essential component. Regarding the composition, Patent Document 2 describes a water-soluble phosphate that suppresses demineralization without negatively impacting remineralization, particularly an enamel demineralization inhibitor containing pyrophosphate or tripolyphosphate. is there. Hexafluorotitanate needs to be periodically applied to the teeth, leaving the user bothersome. Water-soluble phosphates are mainly applied to dentifrices.

  Moreover, as what suppresses demineralization by the acidic food and drink of (2) contacting a tooth surface directly, about the decalcification inhibitor which uses isomaltoligosaccharide reductant as an active ingredient in patent document 3 Patent Document 4 describes a food containing paratinite having a decalcification-inhibiting action and a specific organic acid calcium salt. Isomaltoligosaccharide reductate is close to suppressing demineralization under the surface of the tooth surface under acidic conditions, which is the gist of the present invention, but in order to increase the effect, isomaltoligosaccharide reductate is 30% in food and drink. When this is applied to drinking water, a large amount of a reduced product of isomaltoligosaccharide will be consumed at once. Many sugar reducts (sugar alcohols), including isomalttooligosaccharide reducts, have the physiological effect of “relaxing stomach” when ingested in large amounts, and it is dangerous to drink all 350 ml of beverage, although it varies depending on the individual. There is. Patent Document 4 describes that demineralization is suppressed by promoting remineralization by containing palatinit and a specific organic acid calcium salt. The remineralization action is effective only in the vicinity of neutrality, and is different from the demineralization suppression of the present invention.

Japanese Patent Laid-Open No. 55-35015 JP 7-505400 A JP-A-10-87461 JP 2000-270810 A

  This invention is made | formed in view of the said condition, and it aims at providing the composition for oral cavity which expresses a decalcification inhibitory action on acidic conditions, food / beverage products containing it, etc.

  As a result of intensive studies to solve the above problems, the present inventors have been selected from maltodextrin phosphate, maltodextrin phosphate phosphate, oligosaccharide phosphate, reduced phosphate oligosaccharide, phosphate starch and salts thereof. At least one kind was found and the present invention was completed.

That is, the present invention includes the following inventions.
(1) A composition for inhibiting demineralization comprising at least one selected from maltodextrin phosphate, reduced maltodextrin phosphate, oligosaccharide phosphate, reduced phosphate oligosaccharide, phosphate starch and salts thereof.
(2) The composition for suppressing demineralization according to (1), further comprising at least one water-soluble calcium salt.
(3) The demineralization-suppressing composition according to (1) or (2), wherein the demineralization-suppressing composition has a decalcification-suppressing action at a pH of 4 or less.
(4) The composition for suppressing demineralization according to any one of (1) to (3), which is added to foods, beverages, seasonings, oral preparations, pharmaceuticals, cosmetics, feed, or fertilizers.
(5) Foods, beverages, seasonings, oral preparations, pharmaceuticals, cosmetics, feeds or fertilizers containing the composition for suppressing demineralization according to any one of (1) to (3).

  According to the present invention, decalcification can be suppressed even under acidic conditions of pH 5.5 or less in the oral cavity where demineralization begins. Furthermore, deashing can be suppressed even in a low pH region, which is often unknown in beverages, of pH 4 or less, which is not known at all.

The present invention is described in detail below.
The maltodextrin phosphate (hereinafter referred to as PMD) in the present invention is a phosphorylated saccharide in which at least one phosphate group is bonded to maltodextrin having a glucose polymerization degree exceeding 10, and a reduced maltodextrin phosphate (hereinafter referred to as PMD). (Referred to as reduced PMD) is a phosphorylated saccharide in which at least one phosphate group is bound to a reduced maltodextrin having a glucose polymerization degree exceeding 10. Phosphate oligosaccharide (hereinafter referred to as POS) is a phosphorylated saccharide in which at least one phosphate group is bonded to an oligosaccharide having a glucose polymerization degree of 10 or less, and a reduced phosphate oligosaccharide (hereinafter referred to as reduced POS). Is a phosphorylated saccharide in which at least one phosphate group is bound to a reducing oligosaccharide having a glucose polymerization degree of 10 or less. Phosphoric acid starch is an acidic polysaccharide in which phosphate groups are bonded to starch, and “starch phosphate sodium” defined in food additives is one of them. Starch phosphate sodium is specified to have a bound phosphorus content of 3% by weight or less and an inorganic phosphorus ratio of 20% or less of the total phosphorus. In addition, in this invention, the phosphate starch which is not contained in prescription | regulation of a food additive is also included as object.

  Examples of salts of PMD, reduced PMD, POS and reduced POS, and phosphate starch include alkali metal salts such as sodium salt and potassium salt, alkaline earth metal salts such as calcium salt and magnesium salt, ammonium salt, iron salt and zinc Examples include salts and aluminum salts.

  In the present invention, decalcification is suppressed by using at least one selected from PMD, reduced PMD, POS, reduced POS and salts thereof, preferably a water-soluble calcium salt in combination with the above components.

  PMD, reduced PMD, POS, reduced POS, and salts thereof exhibit a decalcification inhibitory action in a small amount and are not assimilated by oral bacteria, so that an acid that lowers oral pH is not produced. In addition, these substances have a Ca solubilization action that prevents calcium phosphate from precipitating as crystals in a system in which phosphate ions and calcium ions coexist, and at the same time, hydroxyapatite that is a crystal component of calcium phosphate in the teeth is contained in the same system. When present, it is a substance having two actions of remineralization that deposits calcium phosphate on crystalline hydroxyapatite. In addition, these substances promote remineralization as in Patent Document 4, and as a result do not suppress decalcification, but also have an effect of suppressing decalcification itself under acidic conditions.

  When used as a decalcification inhibitor, 0.0001-10 w / w% of at least one selected from PMD, reduced PMD, POS, reduced POS, phosphate starch and salts thereof may be added to food and drink. preferable.

  The effect of the deashing suppression composition of the present invention is enhanced by further blending a water-soluble calcium salt. Examples of the water-soluble calcium salt include calcium lactate, calcium gluconate, calcium pantothenate, calcium citrate, calcium hydrogen phosphate, calcium phosphate, calcium glycerophosphate, calcium chloride, and the like. It is preferable to add 0.001 to 1 w / w%.

  The pH of the food or drink using the deashing suppression composition of the present invention is preferably 3 to 7, more preferably 3 to 6. For adjusting the pH, for example, citric acid, malic acid, tartaric acid, fumaric acid gluconate, succinic acid, acetic acid, lactic acid, adipic acid, itaconic acid, phytic acid, glucono delta lactone, and some salts thereof are used. .

  The composition for inhibiting demineralization of the present invention can be used for foods, beverages, seasonings, oral preparations, pharmaceuticals, cosmetics, feeds, fertilizers and the like.

  EXAMPLES Hereinafter, although an Example and an Example demonstrate this invention concretely, the technical scope of this invention is not limited by the following Example. PMD, POS, reduced PMD, (and) reduced POS and phosphate starch used in the test were prepared by the following method. The amount of bound phosphorus and the average degree of polymerization are shown in Table 1.

(Preparation of test materials)
<Phosphate starch>
Introduce 1200 kg of cornstarch (manufactured by Oji Cornstarch Co., Ltd., 13 wt% water) into the turbulator at a constant flow rate, and simultaneously dissolve 176 kg of monobasic sodium phosphate dihydrate and 32 kg of anhydrous dibasic sodium phosphate in water. 655 kg of phosphoric acid solution (phosphorus content in the solution 6.4% by weight, pH 6.0) was added at a constant flow rate and mixed uniformly. This phosphoric acid mixed starch was dried with a flash dryer until the water content became 6% by weight, and 750 kg of the resulting phosphoric acid-impregnated starch (phosphorus content 3.5% by weight, pH 5.6) was added to a fluidized bed heater (Oji Cornstarch Co., Ltd.). (Made by company). The heated hot air was supplied and fluidized and heated, and the exhausted hot air was discharged out of the fluidized bed. The temperature was raised to 175 ° C. in 30 minutes after the start of heating, and the hot air exhaust continued to be discharged out of the system as it was, and the reaction was performed at 175 ° C. for 120 minutes. After completion of the heating reaction, the air flow was switched to cold air, and the hot air exhaust was continuously discharged out of the system to cool the product temperature to 100 ° C. or lower. The recovered phosphate starch was 700 kg, the bound phosphorus content was 2.8% by weight, and the phosphorylation rate was 81%.

<PMD>
After dissolving 34 g of calcium chloride dihydrate in 71 L of water at 70 ° C., 8 kg of the above phosphate starch was gradually added and dissolved while stirring. After adjusting the pH to 6.0 with sodium hydroxide, 0.05% by weight of starch was added to the super thermostable α-amylase (Termamyl Classic, manufactured by Novozymes) and held for 5 minutes. When the viscosity began to drop, 45 kg of phosphate starch was gradually added. After re-adjusting to pH 6.0 with sodium hydroxide, 0.05% by weight of Termamyl Classic was added to the added starch and held for 10 minutes. Next, the prepared phosphate starch dispersion was treated with a jet cooker under conditions of an inlet temperature of 110 ° C. and a residence time of 5 minutes. The liquid subjected to the jet cooker treatment was collected in a tank, and 0.05% by weight of Termamyl classic was added to the starch and reacted at 60 ° C. for 3 hours. The enzymatic reaction was adjusted to pH 3.5 with hydrochloric acid and completed.

  The enzyme treatment solution was purified in the same manner as in the comparative example, and 8 hours were required for ceramic filtration. Then, it powdered by spray-drying like the comparative example, and obtained 36.2 kg PMD.

<Reduced PMD>
Reduced dextrin 100kg (PO-10, manufactured by Towa Kasei Co., Ltd.) and sodium phosphate solution (15kg monosodium phosphate dihydrate and 2.7kg disodium phosphate dissolved in 400kg water dried powder with a spray dryer This was put into a fluidized bed, heated to 170 ° C. and then roasted for 2 hours, and the resulting sample was made into a 20% aqueous solution and 10% by weight of powdered activated carbon was added to the solid, and stirred at 60 ° C. for 2 hours. After that, the residue and activated carbon were removed with a ceramic filter.When the filtration feed liquid decreased, pure water was added and filtration was performed until the Brix of the permeate was 1 or less. Then, the solution was adjusted to pH 6.2 with 10% by weight sodium hydroxide, filtered through a 0.45 μm polysulfone membrane filter, and dried with a spray dryer. Sueka to obtain a reducing PMD.

<POS>
POS was obtained according to the method of Example 1 of JP-A-2002-325556.

<Reduced POS>
Reduced POS was obtained according to the method of Example 10 of JP-A No. 2002-325556 using the POS obtained above as a starting material.

(Experimental example)
The following deashing suppression test was conducted to determine and evaluate the deashing suppression rate.

(Decalcification suppression test)
A test solution having a pH of 4.5 shown in Table 2 was prepared, and 5 mg of hydroxyapatite (SIGMA) was added to 10 ml of the control solution and the test solution and stirred, followed by decalcification at 36 ° C. for 1 hour. After completion of the reaction, the mixture was centrifuged at 12,000 rpm for 3 minutes, the soluble calcium concentration of the supernatant was measured with a calcium measurement kit (Calcium C Test Wako manufactured by Wako Pure Chemical Industries), and the decalcification inhibition rate was calculated by the following formula. Unless otherwise indicated,% is w / v%.
Decalcification rate = (calcium concentration of test solution or control solution ^* / calcium concentration when all of the added hydroxyapatite is dissolved) × 100
* In the test in which water-soluble calcium salt is added, the concentration obtained by subtracting the calcium content of the added calcium salt is used. Demineralization inhibition rate = (1− (decalcification rate of test solution / decalcification rate of control solution)) × 100
The results are shown in Table 3.

As can be seen from the results of the experimental examples, the addition of PMD, reduced PMD, POS, reduced POS, and phosphate starch can significantly suppress decalcification and confirm that these substances have a strong demineralization inhibitory effect. It was. It was also confirmed that the action was enhanced by using a water-soluble calcium salt in combination.
Next, the example which utilized this invention composition for food and drink is shown.

(Example 1)
Food and drink (acidic water: lemon tea)
5 mg of hydroxyapatite (SIGMA) was added to both solutions as a test solution in which 0.1 g of PMD was dissolved in 10 ml of lemon tea (pH 3.9) which is an acidic drinking water, After stirring, the reaction was decalcified at 36 ° C. for 1 hour. After completion of the reaction, the mixture was centrifuged at 12000 rpm for 3 minutes, and the soluble calcium concentration of the supernatant was measured. The demineralization inhibition rate was calculated from the formula for the demineralization inhibition test described above. The decalcification inhibition rate was 40%.

(Example 2)
Food and drink (acidic water: lemon tea)
The same operation as in Example 1 was performed except that 0.1 g of PMD and 0.01 g of calcium lactate were dissolved in 10 ml of lemon tea (pH 3.9) which is acidic drinking water. The decalcification inhibition rate was 52%.

(Comparative Example 1: PMD-free example)
The same operation as in Example 1 was performed except that 0.1 g of sugar was dissolved instead of PMD. The demineralization inhibition rate was 0%.

(Example 3)
Food and drink (acidic drinking water: black coffee with sugar)
Hydroxyapatite (SIGMA) was used as a test solution in which 0.01 g of PMD was dissolved in 10 ml of sugar-containing black type coffee (pH 5.3), which is acidic drinking water, and in which nothing was added as a control solution. After 5 mg was added and stirred, the reaction was decalcified at 36 ° C. for 1 hour. After completion of the reaction, the mixture was centrifuged at 12000 rpm for 3 minutes, and the soluble calcium concentration of the supernatant was measured. The decalcification inhibition rate was 93%.

(Comparative example 2: PMD additive-free example)
The same operation as in Example 3 was performed except that 0.01 g of glucose was dissolved instead of PMD. The demineralization inhibition rate was 0%.

Example 4
Food and drink (acidic drinking water: fruit juice)
5 mg of hydroxyapatite (SIGMA) was added to both solutions as a test solution in which 0.1 g of PMD was dissolved in 10 ml of fruit juice (pH 3.7) which is acidic drinking water, and nothing added. After stirring, the reaction was decalcified at 36 ° C. for 1 hour. After completion of the reaction, the mixture was centrifuged at 12000 rpm for 3 minutes, and the soluble calcium concentration of the supernatant was measured. The demineralization inhibition rate was 21%.

(Example 5)
Food and drink (acidic drinking water: fruit juice)
The same operation as in Example 4 was performed except that 0.01 g of calcium lactate and 0.1 g of PMD were dissolved in 10 ml of fruit juice (pH 3.7) as acidic drinking water. The demineralization inhibition rate was 35%.

(Comparative example 3: PMD additive-free example)
The same operation as in Example 4 was performed except that 0.1 g of sugar was dissolved instead of PMD. The demineralization inhibition rate was 0%.
The results of Examples 1 to 5 and Comparative Examples 1 to 3 are summarized in Table 4.

(Example 6)
In this example, a decalcification suppression test was performed by replacing the hydroxyapatite (SIGMA) with a hydroxyapatite molded product (CELLYARD HA scaffold manufactured by PENTAX), which is a structure closer to a tooth.
Food and drink (acidic water: sports drink)
A test drink is prepared by dissolving 0.01 g of PMD in 10 ml of sports drink (pH 3.5), which is an acidic drinking water, and one tablet of hydroxyapatite molded product is added to both solutions, with nothing added as a control solution, and stirred. After that, decalcification was performed at 36 ° C. for 2 hours. After completion of the reaction, the soluble calcium concentration of the supernatant was measured. The demineralization inhibition rate was calculated from the formula for the demineralization inhibition test described above. The decalcification inhibition rate was 47%.

(Example 7)
The same operation as in Example 6 was performed except that 0.05 g of PMD was dissolved in 10 ml of sports drink (pH 3.5) which is acidic drinking water. The decalcification inhibition rate was 65%.

(Example 8)
The same operation as in Example 6 was performed, except that 0.01 g of calcium chloride was dissolved together with 0.03 g of PMD in 10 ml of sports drink (pH 3.5) which is an acidic drinking water. The decalcification inhibition rate was 65%.

(Comparative example 4: PMD additive-free example)
The same operation as in Example 6 was performed except that 0.1 g of xylitol was dissolved instead of PMD. The demineralization inhibition rate was 0%.

Example 9
Food and drink (acidic drinking water: peach juice)
A solution of 0.05 g of reduced PMD dissolved in 10 ml of peach juice (pH 3.6), which is acidic drinking water, was added to the test solution, and one tablet of hydroxyapatite molded product was added to both solutions. After stirring, the reaction was decalcified at 36 ° C. for 2 hours. After completion of the reaction, the soluble calcium concentration of the supernatant was measured. The demineralization inhibition rate was calculated from the formula for the demineralization inhibition test described above. The decalcification inhibition rate was 37%.

(Comparative Example 5: PMD-free example)
The same operation as in Example 8 was performed except that 0.1 g of paratinite was dissolved instead of reduced PMD. The demineralization inhibition rate was 0%.
The results of Examples 6 to 9 and Comparative Examples 4 and 5 are summarized in Table 5.

Claims (5)

  A composition for suppressing demineralization, comprising at least one selected from maltodextrin phosphate, reduced maltodextrin phosphate, oligosaccharide phosphate, reduced phosphate oligosaccharide, phosphate starch and salts thereof.   Furthermore, the composition for demineralization suppression of Claim 1 containing at least 1 sort (s) of water-soluble calcium salt.   The demineralization-suppressing composition according to claim 1 or 2, wherein the demineralization-suppressing composition has a decalcification-suppressing action at a pH of 4 or less.   The composition for demineralization suppression according to any one of claims 1 to 3, which is added to foods, beverages, seasonings, oral preparations, pharmaceuticals, cosmetics, feed or fertilizers.   A food, beverage, seasoning, oral preparation, pharmaceutical, cosmetic, feed or fertilizer containing the composition for suppressing demineralization according to any one of claims 1 to 3.