JP2005330212A - Skin function ameliorant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a preparation with which a consumer realizes an antiaging function. <P>SOLUTION: The subject skin function ameliorant comprises an extract of Malus baccata var. mandschuria. The ameliorant is an antiaging preparation which satisfactorily exhibits bleaching effect and prevention effect on wrinkles of the extract of Malus baccata var. mandschuria. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a function improver characterized by blending an active ingredient extracted from Japanese apple (Malus baccata var. Mandschuria), and more specifically, blending an active ingredient having both a whitening effect and an anti-wrinkle effect. The present invention relates to an anti-aging preparation having a function of normalizing the skin condition.

  The modern society is also called a stress society, and although there is a marked difference (improvement) in material richness from the past, there is also a trend that external conditions such as the living environment are increasingly getting worse. is there. For this reason, the health consciousness in recent years has been deeply rooted, and efforts have been made to maintain an internally healthy body and spirit while paying attention to eating habits. For example, ingestion of health foods and functional foods appears.

  On the other hand, appearance problems, that is, skin care using various cosmetics to maintain healthy, beautiful and youthful skin, massage and relaxation by aesthetics, etc. have become common, and a series of life It is becoming established as a style. However, since many of these cares themselves are complicated, a vicious circle in which they feel tired from continuation of care and generate new stress has also become prominent. Moreover, none of the conventional measures are out of symptomatic treatment, and the effect of care for emerging skin troubles is only temporary, and it has become a cosmetic experience for troubled consumers. Absent.

As a so-called anti-aging material, plant-derived research has been extensive from the viewpoint of safety, but materials that can simultaneously realize a whitening effect and an anti-wrinkle effect have been obtained in terms of restoring a healthy skin condition. Absent.
Therefore, it has been demanded to provide materials that can easily realize daily care and can realize improvement of skin function.

The apples we eat daily are classified into the genus Malus pumila, and there are many known examples of their application to cosmetic ingredients. This is an area that can be expected.
In particular, conventional apple extracts have a moisturizing function, and there is no whitening effect and wrinkle prevention effect from the viewpoint of preventing aging that is currently required. This is insufficient as a material for improving skin function (see, for example, Patent Documents 1 to 4).
Japanese Patent Laid-Open No. 55-19205 JP-A-9-175982 JP 2000-264828 A JP 2001-213750 A

  The present invention is based on such a current situation, and an object of the present invention is to provide a preparation that allows consumers to realize an anti-aging function.

  As a result of continual search to solve the above-described conventional problems, the present inventor found specific aptitude as a desired anti-aging material for Japanese apple (Malus baccata var. Mandschuria), and completed the present invention. It came to do.

That is, the skin function improving agent of the present invention is characterized in that it contains an active ingredient extracted from Japanese apple (Malus baccata var. Mandschuria).

  ADVANTAGE OF THE INVENTION According to this invention, the formulation which has a whitening effect and the wrinkle prevention effect, and has the function to normalize a skin state is provided.

Hereinafter, embodiments of the present invention will be described in detail.
The skin function-improving agent of the present invention is characterized in that it contains an active ingredient extracted from a plant identified from among the Rosaceae plants, a Japanese apple (Malus baccata var. Mandschuria).

  The extract of Japanese apple (Malus baccata var. Mandschuria) used in the present invention can be obtained by extracting Japanese apple (Malus baccata var. Mandschuria) with water, a hydrophilic solvent such as ethanol and 1,3-butylene glycol. Extracts are particularly preferred. The suitable manufacturing method of the Japanese apple extract which is an active ingredient of this invention is described below.

A hydrophilic solvent is added to Japanese apple (Malus baccata var. Mandschuria) at a ratio of 1: 1 to 1:10, and the mixture is extracted by immersion or stirring for 1 to 48 hours at a temperature of 0 to 70 ° C. After squeezing the extract, it can be subjected to a purification process such as filtration or centrifugation to obtain the extract of the apple of the present invention.

The starting raw material apple (Malus baccata var. Mandschuria) is usually pulverized to about 1000 microns or less in advance by a pulverizer in consideration of extraction efficiency, but the pulverized raw material itself is easily oxidized over time. It is sometimes preferred to prepare.

Examples of the hydrophilic solvent include, in addition to water, methanol, ethanol, propanol, 1,3-butylene glycol, propylene glycol, glycerin and the like, and any of them can be used. In view of the above, water, ethanol, and 1,3-butylene glycol are most preferable. During extraction, it is preferable to adjust the pH to 4.0 to 9.0 in that the fraction of the active ingredient can be obtained efficiently. In the extraction step, extreme heating is avoided in order to stabilize the retention of active ingredients, and the conditions for stirring are arbitrary.

The form of the preparation can be provided externally or as a food, but as an external preparation, generally an acceptable base is selected and applied directly to the affected area. In this case, in addition to homogeneous preparations represented by lotions and essences, general emulsification systems such as O / W and W / O types represented by creams and emulsions, W / O / W, O / W / O-type special multi-layer emulsions, other types such as pastes, ointments and tinctures, sprays such as aerosols and sprays, patch types such as poultices and plasters, etc. The basic base is also used in combination with other components and is not particularly limited.

In foods, various forms such as solids and liquids, for example, capsules, tablets, granules, powders, syrups, drinks and the like can be provided in known forms that can be taken orally.

In these present inventions, the amount of Japanese apple extract is used for cosmetics such as creams, lotions, milky lotions, packs, lotions, essences, and external preparations such as ointments, poultices, and plasters. In any case, it is blended in the range of 0.01 to 50% by weight, preferably 0.05 to 5% by weight with respect to the whole preparation. When the blending amount is “less than 0.01% by weight”, the skin function improving action is insufficient. Further, even if it exceeds 50% by weight, there is no particular difference in effect from the case of less than that, and in this case, there is a problem that it is economically disadvantageous.

  In the present invention, various known active ingredients that are usually used, for example, quercetin, glutathione, hydroquinone and derivatives thereof known as whitening agents, phenolic compounds such as condensed tannins, caffeic acid and ellagic acid As peripheral vasodilators, vitamin E, vitamin E nicotinate, nicotinic acid, nicotinic acid amide, nicotinic acid benzyl and other vitamins, ginger tincture, chili pepper tincture, anti-inflammatory agents are corticosteroids, ε-aminocaproic acid, Various compounds such as lysozyme chloride, glycyrrhizin, allantoin, and other extracts derived from animals, plants, and microorganisms such as placenta extract, licorice extract, purple root extract, and lactic acid bacteria culture extract, etc., as long as the effects of the present invention are not impaired. In addition, it should be added as appropriate according to the purpose at the time. Can.

  Furthermore, in addition to these known active ingredients, the external preparation of the present invention includes base ingredients such as fats and oils, as well as known humectants, preservatives, antioxidants, chelating agents, and pH adjusters as necessary. Various additives such as fragrances and coloring agents can be used in combination as long as the effects of the present invention are not impaired.

  EXAMPLES Next, the present invention will be described by way of examples, but these disclosures show preferred embodiments of the present invention and do not limit the present invention in any way.

<Production Example 1>
1 part of ground apple crush was adjusted to 3 parts water and pH 6.0 and stirred at 40 ° C. for 3 hours. After cooling to room temperature, the mixture was filtered to obtain a clear Japanese apple apple extract.

<Production Example 2>
Add 3 parts of ethanol to 1 part of ground apple and add it at 5 ° C overnight or longer for extraction. This liquid was filtered to obtain a clear Japanese apple extract.

<Production Example 3>
3 parts of 1,3-butylene glycol was added to 1 part of a ground apple, and the mixture was extracted by stirring at 70 ° C for 1 hour. This liquid was heated to 40 ° C. and filtered, and after cooling, a clear Japanese apple extract was obtained.

<Test Example 1> Tyrosinase Activity Inhibiting Action a) Test Method As shown in Table 1, the above Production Examples 1 to 3 were used as samples of the examples. Moreover, the apple extract of the comparative example used the sample which replaced the palm apple with the commercial apple [Jonagold (Malus pumila)] in manufacture example 1 thru | or 3.

マッシュルーム由来タイロシネース（ＳＩＧＭＡ社、３０００Ｕｎｉｔｓ／ｍｇ）のミクロスパーテル１杯を１０ｍＬの０．０５Ｍリン酸緩衝液に加え、タイロシネース液とする。ヒメリンゴ抽出液試料の５０μＬに、０．０５Ｍリン酸緩衝液（ｐＨ６.８）の５７７μＬ、タイロシネース液４０μＬを１ｍＬのセルに順次加える。これに、１０ｍＭ ＤＯＰＡ液３３３μＬを加え、３７℃で２分間反応させ、４７５ｎｍの吸光度を測定した。比較例として市販のリンゴエキスを同様の方法で評価した。ヒメリンゴ抽出液試料及び市販のリンゴエキス試料のタイロシネース活性抑制率は以下の式にて算出した。

抑制率(％) ＝
１−(試料の終了時の吸光度−試料の開始時の吸光度）／（コントロールの終了時の吸光度−コントロールの開始時の吸光度）×100

A glass of mushroom-derived tyrosinase (SIGMA, 3000 Units / mg) of microspatel is added to 10 mL of 0.05 M phosphate buffer to form a tyrosinase solution. Add 577 μL of 0.05 M phosphate buffer solution (pH 6.8) and 40 μL of tyrosinase solution to 50 μL of the Japanese apple extract sample sequentially in a 1 mL cell. To this, 333 μL of 10 mM DOPA solution was added and reacted at 37 ° C. for 2 minutes, and the absorbance at 475 nm was measured. As a comparative example, a commercially available apple extract was evaluated by the same method. The tyrosinase activity inhibitory rate of a Japanese apple extract sample and a commercially available apple extract sample was calculated by the following formula.

Suppression rate (%) =
1- (absorbance at the end of the sample-absorbance at the start of the sample) / (absorbance at the end of the control-absorbance at the start of the control) × 100

b) Test results The test results are shown in Table 2.

ｃ）考察
本発明の実施例については、いずれも優れたタイロシネース活性抑制作用を示したのに対し、比較例では、抑制効果は認められなかった。このことから、本発明のヒメリンゴ抽出液は、従来のリンゴエキスに対して優れた効果を有することが明らかである。

c) Consideration All the examples of the present invention showed excellent tyrosinase activity inhibitory action, whereas the comparative example showed no inhibitory effect. From this, it is clear that the Japanese apple extract of the present invention has an excellent effect on the conventional apple extract.

<Test Example 2> Radical scavenging action a) Test method The same samples as in Test Example 1 were used. Table 3 shows the evaluation concentration of each sample.

各試料の２ｍＬに、エタノールの２ｍLを加える。０．５ｍM DPPH(ナカライテスク社)の1ｍLを加え、３０分放置後、５１７ｎｍの吸光値を測定する。ラジカル消去率は以下の計算式にて計算する。
ラジカル消去率(%)＝
(コントロールの吸光値−試料の吸光値) ／コントロールの吸光値×１００

Add 2 mL of ethanol to 2 mL of each sample. Add 1 mL of 0.5 mM DPPH (Nacalai Tesque), leave it for 30 minutes, and measure the absorbance at 517 nm. The radical scavenging rate is calculated by the following formula.
Radical scavenging rate (%) =
(Absorption value of control−Absorption value of sample) / Absorption value of control × 100

b) Test results Table 4 shows the test results.

ｃ）考察
本発明の実施例については、いずれも優れたラジカル消去作用を示したのに対し、比較例では、同様の作用は認められたものの、その効果は弱かった。このことから、本発明のヒメリンゴ抽出液は、従来のリンゴエキスに対して優れた効果を有することが明らかである。
試験例１の結果を併せて考えると、その効果の差は歴然である。すなわち、本発明のヒメリンゴ抽出液は、従来のリンゴ抽出液には認められなかったタイロシネース活性抑制作用とラジカル消去作用の双方を兼ね備えているため、老化防止用の素材として最適なものであるということができる。

c) Discussion Although all of the examples of the present invention showed excellent radical scavenging action, in the comparative example, the same action was observed, but the effect was weak. From this, it is clear that the Japanese apple extract of the present invention has an excellent effect on the conventional apple extract.
Considering the result of Test Example 1 together, the difference in the effect is obvious. That is, the Japanese apple extract of the present invention has both a tyrosinase activity inhibitory action and a radical scavenging action not found in conventional apple extracts, and is therefore an optimum material for preventing aging. Can do.

Formulation Example 1 Lotion

Formulation Example 2 Essence

Formulation Example 3 Cream

Formulation Example 4 Cream Pack

Formulation Example 5 Drink

Formulation Example 6 Tablets

これら処方例1乃至７は、いずれも本発明の目的を達成する効果を有していることが確認された。

Formulation Example 7 Candy

It was confirmed that any of these Formulation Examples 1 to 7 has an effect of achieving the object of the present invention.

Claims (4)

An agent for improving skin function, comprising an active ingredient extracted from Japanese apple (Malus baccata var. Mandschuria). The skin function improving agent according to claim 1, which is an active ingredient extracted from water or a hydrophilic solvent. 3. The skin function improving agent according to claim 1, wherein the hydrophilic solvent is ethanol or 1,3-butylene glycol. 4. The skin function improving agent according to claim 1, wherein the form of the skin function improving agent is for external use or food.