JP2005263770A - Method for producing powdery 1,5-d-anhydrofructose - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for readily and inexpensively producing powdery 1,5-D-anhydrofructose. <P>SOLUTION: The method for producing the powdery 1,5-D-anhydrofructose comprises spraying and drying 1,5-D-anhydrofructose-containing composition obtained by mixing 1 pt. wt. 1,5-D-anhydrofructose with 0.2-10.0 pts. wt. degraded starch as a powdery base. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a process for producing powdered 1,5-D-anhydrofructose.

  1,5-D-anhydrofructose (hereinafter referred to as 1,5-AF) is a saccharide that can be prepared by enzymatic degradation of starch, and exhibits properties not found in other saccharides. Antioxidants that can be safely added to foods (see [Patent Document 1]), antibacterial agents that are particularly effective against Bacillus subtilis and lactic acid bacteria (see [Patent Document 2]), and anti-aging agents for starch (see [Patent Document 3]) ), Sugar donors to amino compounds such as proteins (see [Patent Document 4]), and food coloring agents (see [Patent Document 5]), etc. This monosaccharide is also a precursor of the antibiotic pyrone microthecin (see [Patent Document 6]).

  In Patent Document 1, a method for isolating α-1,4-glucan lyase from fungi such as Morcella costa or Morchella vulgaris and algae such as Gracilariapsis lemaneformis, and a solution of starch or starch degradation product as α-1,4- A method for producing 1,5-AF easily, inexpensively and in large quantities by degrading with glucan lyase is disclosed.

  1,5-AF is a carbohydrate expected to be used in various fields. 1,5-AF has low stability in the state of an aqueous solution, and in the storage of a preparation containing 1,5-AF, the product quality such as a decrease in 1,5-AF content with time or coloring of the preparation itself Changes occur. In order to reduce the change in quality, storage at a lower temperature is required. However, when distribution in the market is taken into consideration, there are disadvantages such as an increase in cost due to storage costs and a limitation of sales routes.

On the other hand, spray drying is a method generally used in pulverization of starch sugar, but whether spray drying is possible or not depends greatly on the degree of polymerization of starch sugar. In other words, maltodextrin with a high degree of polymerization is relatively easy to spray dry, but glucose, which is a monosaccharide, and maltose, which is a disaccharide, are difficult to spray and are highly hygroscopic even when forced to dry. Therefore, spray drying is not performed in industrial production. Crystalline products are common as powder products of these sugars. As with glucose and maltose, spray drying alone is difficult for 1,5-AF, and as a method for pulverization, it has been reported that a high-purity 1,5-AF solution is pulverized by freeze-drying. However, this method has disadvantages that the production cost is very high and the hygroscopicity of the dried product is very high.
Special table hei 9-505988 JP 2001-89377 A Japanese Patent Laid-Open No. 2001-333712 JP 2001-213891 A JP 2002-27945 French Patent Application Publication No. 2617502 J. et al. Apply. Glycosci. , Vol 46, No 4, 439-444 (1999)

  An object of the present invention is to provide a method for pulverizing 1,5-AF easily and inexpensively.

  Another object of the present invention is to provide powdered 1,5-AF.

  Still other objects and advantages of the present invention will become apparent from the following description.

  As a result of intensive research on pulverization of 1,5-AF, the present inventors have found that 1,5-AF can be easily pulverized by mixing with a starch degradation product and spray-drying. The present invention has been reached based on the facts.

  That is, according to the present invention, the above-mentioned objects and advantages of the present invention are as follows. First, 0.2 to 10.0 parts by weight of a starch decomposition product or the like is mixed with 1 part by weight of 1,5-AF. This is achieved by a method for producing powder 1,5-AF, characterized by spray-drying a 5-AF-containing composition.

  In addition, according to the present invention, the above-mentioned objects and advantages of the present invention are, secondly, 1,5-AF obtained by decomposing starch or starch degradation product with α-1,4-glucan lyase, and the decomposition residue. This is achieved by a method for producing powder 1,5-AF, which comprises spray-drying a 1,5-AF-containing composition comprising maltodextrin as a product.

  Furthermore, according to the present invention, the above objects and advantages of the present invention are achieved thirdly by providing the powder 1,5-AF obtained by the above production method.

  These are described in detail below.

  In the present invention, 1,5-AF used as a raw material can be obtained by the following known methods.

  After adding water to the red algae Gracilaria verrucosa and grinding it, the α-1,4-glucan lyase enzyme solution prepared by the starch adsorption method from the extract obtained by solid-liquid separation was allowed to act on the glutinous rice starch aqueous solution. Thereafter, ethanol was added to a concentration of 50%, and unreacted starch was precipitated and removed, and then the supernatant was concentrated to obtain a sample having a 1,5-AF purity of 95% or more.

  When spray drying is performed without mixing the thus obtained 1,5-AF having a purity of 95% or more with the powdered base material, it is impossible to form a powder regardless of the spray drying conditions. Met.

  In the method for producing powder 1,5-AF of the present invention, by spray-drying a 1,5-AF-containing composition obtained by mixing 1,5-AF prepared by the above method and a starch degradation product The powder 1,5-AF can be produced easily and inexpensively.

  The mixing ratio of the starch decomposition product is preferably 0.2 to 10.0 parts by weight and more preferably 0.5 to 3.0 parts by weight with respect to 1,5-AF 1 part by weight.

  Further, a reaction solution obtained by allowing an α-1,4-glucan lyase enzyme solution to act on the maltodextrin solution described above, that is, a 1,5-AF-containing composition comprising 1,5-AF and undegraded maltodextrin It is also possible to spray and dry the powder as it is.

  In the pulverization method of the present invention, the solid content of the 1,5-AF-containing composition that is the spray-drying stock solution is preferably 5 to 70% by weight, and 30 to 60% by weight in consideration of cost and drying efficiency. It is more preferable that

  As the spray dryer used for drying, a device generally used for spray drying, such as a nozzle type or a rotating disk type, can be used.

  The temperature of the hot air blown into the spray dryer is preferably from 70 to 200 ° C. in consideration of conversion or decomposition of 1,5-AF into other compounds, product coloring, and the like during drying.

  Thus, the 1,5-AF powder product obtained by the method of the present invention was a white fine powder, and its water content was about 1 to 3%. When this powder was confirmed with a microscope, it was spherical with a relatively uniform particle diameter, and was excellent in fluidity, solubility and dispersibility. Since the 1,5-AF powder product has very high storage stability compared to the liquid product, it can be stored at room temperature or higher.

  1,5-AF powder products should be added to various compositions such as foods, beverages, feeds, cosmetics, and pharmaceuticals as antibacterial agents, antioxidants, anti-discoloring agents, and taste improvers, etc. The addition amount is not particularly limited.

    According to the present invention, 1,5-AF can be easily and inexpensively manufactured by mixing 1,5-AF with a starch decomposition product as a powdered base material and spray drying. . The 1,5-AF powder product thus obtained has improved storage stability and can be stored at room temperature for a long time.

  The following examples further illustrate the present invention, but the present invention is not limited thereto. In addition,% used in the following examples means weight%. Moreover, the Okawahara Kako Co., Ltd. product L-8 type (rotary disk type) was used for the spray dryer.

Example 1
After adjusting the concentration of the 1,5-AF solution to 30%, spray drying was attempted under the conditions described in Table 1. However, it became fine droplets and a dry powder was not obtained. Furthermore, spray drying under various concentration conditions and drying conditions was attempted, but powdering was impossible.

Example 2
A 1,5-D-anhydrofructose solution with a concentration of 30% and a maltodextrin (DE = 16) solution with a concentration of 30% were mixed at a ratio of 1: 0.8, and then dried under the conditions described in Table 2. As a result, a fine spherical dry powder could be obtained. The resulting product had a moisture content of 3.11%.

Example 3
A 1,5-D-anhydrofructose solution with a concentration of 30% and a maltodextrin (DE = 16) solution with a concentration of 30% were mixed at a ratio of 1: 1.5, and then dried under the conditions described in Table 3. As a result, a fine spherical dry powder could be obtained. The resulting product had a moisture content of 1.80%.

Example 4
A sugar solution (sugar composition: glucose 8%, 1,5-AF 36%, undegraded maltodextrin 56%) obtained by allowing glucan lyase to act on a maltodextrin aqueous solution of DE12 was dried under the conditions described in Table 4. As a result, a fine spherical dry powder could be obtained. The resulting product had a moisture content of 1.72%.

Claims (8)

A process for producing powdered 1,5-D-anhydrofructose comprising spray-drying a 1,5-D-anhydrofructose-containing composition comprising 1,5-D-anhydrofructose and a starch degradation product. The 1,5-D-anhydrofructose-containing composition contains 0.2 to 10.0 parts by weight of a starch degradation product with respect to 1 part by weight of 1,5-D-anhydrofructose. The manufacturing method of Claim 1. The starch-decomposed product is contained in the 1,5-D-anhydrofructose-containing composition in an amount of 0.5 to 3.0 parts by weight based on 1,5-D-anhydrofructose. The production method described in 1. The process according to claim 1, wherein the degree of polymerization of glucose residues constituting the starch degradation product is 7 or more. A 1,5-D-anhydrofructose-containing composition was obtained by decomposing starch or starch degradation product with α-1,4-glucan lyase, and 1,5-D-anhydrofructose and The manufacturing method of Claim 1 which consists of maltodextrin which is the decomposition | disassembly residue. The manufacturing method in any one of Claims 1-5 whose solid content of a 1, 5-D-anhydrofructose containing composition is 5 to 60 weight%. The manufacturing method in any one of Claims 1-6 whose temperature of the hot air in spray drying is 70-200 degreeC. Powder 1,5-D-anhydrofructose obtained by the production method according to claim 1.