JP2005220078A - Antibacterial composition - Google Patents
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- JP2005220078A JP2005220078A JP2004029893A JP2004029893A JP2005220078A JP 2005220078 A JP2005220078 A JP 2005220078A JP 2004029893 A JP2004029893 A JP 2004029893A JP 2004029893 A JP2004029893 A JP 2004029893A JP 2005220078 A JP2005220078 A JP 2005220078A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
Description
本発明は、果実のイチゴ果から得られる搾汁、または極性溶媒抽出エキスと、担体とからなる抗菌組成物、並びに抗菌作用物質として有用なイチゴ果の極性溶媒抽出エキスに関する。 The present invention relates to an antibacterial composition comprising a juice obtained from strawberry fruit or a polar solvent extract and a carrier, and a polar solvent extract extract of strawberry fruit useful as an antibacterial agent.
いわゆる果実としてのイチゴは、バラ科オランダイチゴ属に属するオランダイチゴ(学名:Fragaria × magna Thuill.やF. chiloensis. (L)Duch. Var ananasa Bailey等)のイチゴ果である。このイチゴ果は、膨らんだ花床(花托)とその上についている果実の痩果とからなっていて、偽果と称される。オランダイチゴは、イチゴ果(偽果)が甘く、芳香を放ち、形状も大きいことから、現在では広く一般に栽培されている食用植物である。オランダイチゴは、17ないし18世紀にヨーロッパで作出され、日本には江戸時代天保年間にオランダ人によってもたらされたと云われている。
オランダイチゴのイチゴ果は、主として生で食用とされるほか、イチゴシロップ、イチゴジャム、ゼリーなどの原料とされる。また、古来より、種々の天然物、特に植物材料に含まれる生理活性物質の探索研究も盛んに行われており、オランダイチゴの葉には、タンニン、フラボノイド、イチゴ果には、糖、リンゴ酸、クエン酸等が含まれている。また、イチゴ果にビタミンCであるアスコルビン酸が多く含まれていることもよく知られている。これらのイチゴ果および葉を、収斂、抗利尿、抗壊血病、強壮、緩下薬に、またイチゴ果を滋養、清涼薬とすることも知られている(例えば、非特許文献1参照)。
Strawberries as so-called fruits are strawberry fruits of Dutch strawberry (scientific name: Fragaria × magna Thuill. Or F. chiloensis. (L) Duchen. Var ananasa Bailey, etc.) belonging to the Rosaceae family. This strawberry fruit consists of a swelled flower bed (flower bud) and the fruit berry on it, and is called a false fruit. Dutch strawberries are edible plants that are widely cultivated nowadays because strawberry fruits (pseudofruits) are sweet, fragrant, and large in shape. Dutch strawberries were produced in Europe in the 17th and 18th centuries, and are said to have been brought to Japan by the Dutch during the Edo period Tenpo.
The Dutch strawberry fruit is used mainly as raw edible and also as a raw material for strawberry syrup, strawberry jam and jelly. In addition, since ancient times, research on bioactive substances contained in various natural products, especially plant materials, has been actively conducted. The Dutch strawberry leaves have tannins, flavonoids, strawberry fruits have sugar, malic acid. , Citric acid and the like. It is also well known that strawberry fruits contain a large amount of vitamin C ascorbic acid. It is also known that these strawberry fruits and leaves are used as astringent, antidiuretic, anti-scurvy, tonic, laxative, and strawberry fruits are used as nourishing and refreshing drugs (see, for example, Non-Patent Document 1). .
特許文献1には、ドーパミン含有の活性酸素消去種と、該消去種の作用を促進する活性酸素消去促進種を含む活性酸素消去材、フィルター、健康食品、食品、および飲料であって、その活性酸素消去種の一つとして「いちご」が含まれることが記載されている。
また、特許文献2には、バラ科に属する果実の未熟果実より搾汁および/または抽出され、かつその画分が精製されて成る果実ポリフェノール、その製造方法、酸化防止剤、血圧降下剤、抗変異原性作用剤、アレルギー剤、抗う蝕剤及び消臭剤等の発明が記載されており、従来技術として茶のポリフェノール(カテキン類)においては抗菌、抗う蝕、消臭等広範な生理作用を有することが認知されつつあることが記載されているが、当該発明においてはリンゴ、ナシ、モモなどのバラ科木本の未熟果実を記載しているだけで、イチゴについては実施例その他の具体的記載は一切ない。
さらに、特許文献3および4には、果実、果種等から得られたポリフェノール画分を逆相分離用液体カラムクロマトグラフィーにより分離したときに得られるピークであって、その内のメジャーなピークの中で、最後に溶出されるピークに相当する成分を主成分として含む静菌剤、該静菌剤を含有する飲料、飲料添加剤への処理方法等の発明について記載されている。しかしながら、実施例において専らポリフェノール含有果実、果種として記載されているのはブドウ種子抽出物を記載しているだけで、イチゴについての具体的開示は示されていない。
同様に特許文献5には、バラ科に属する果実の未熟果実より搾汁および/または抽出されて成ることを特徴とする果実ポリフェノール含有果汁をその発明の要旨とする技術が記載されているが、リンゴ、ナシ、モモなどのバラ科木本の未熟果実を記載しているだけで、イチゴ果の生理作用について具体的記載がないのは前記と同様である。
Patent Document 1 discloses an active oxygen scavenging species containing dopamine, and an active oxygen scavenging material, a filter, a health food, a food, and a beverage containing active oxygen scavenging promoting species that promote the action of the scavenging species. It is described that “strawberry” is included as one of the oxygen-erasing species.
Patent Document 2 discloses a fruit polyphenol obtained by squeezing and / or extracting from an immature fruit belonging to the family Rosaceae and purifying a fraction thereof, a production method thereof, an antioxidant, a blood pressure lowering agent, Inventions such as mutagenic agents, allergic agents, anti-cariogenic agents, and deodorants have been described, and tea polyphenols (catechins) have a wide range of physiological effects such as antibacterial, anti-cariogenic, and deodorant as conventional techniques. Although it is described that it is being recognized that it has an immature fruit of a rose family tree such as apple, pear, peach, etc. in the present invention, examples of strawberry and other specific examples are described. There is no description.
Furthermore, Patent Documents 3 and 4 include peaks obtained when a polyphenol fraction obtained from fruits, fruit seeds, and the like is separated by liquid column chromatography for reverse phase separation, and major peaks among them are obtained. Among them, inventions such as a bacteriostatic agent containing a component corresponding to the peak eluted last as a main component, a beverage containing the bacteriostatic agent, and a method for treating a beverage additive are described. However, only the grape seed extract is described in the examples as the polyphenol-containing fruit and fruit type, and no specific disclosure about strawberry is shown.
Similarly, Patent Document 5 describes a technology that includes fruit polyphenol-containing fruit juice characterized by being extracted and / or extracted from an immature fruit of a fruit belonging to the family Rosaceae, Only the immature fruits of the Rosaceae tree such as apples, pears, peaches, etc. are described, but the physiological action of the strawberry fruits is not specifically described as described above.
一方、抗菌活性を利用する食品保存剤あるいは食品添加物、あるいはにきび等の予防・治療を目的に化粧料に配合される抗菌組成物、虫歯、歯周病の予防・治療を目的に口腔衛生料に配合される抗菌組成物、抗菌活性を利用する消臭剤・脱臭剤等においても、従来より安全性が高い生薬を使用する試みが種々行われている。例えば、飲食物を長期間に保存する方法としては、従来より、加熱、乾燥、塩蔵、冷凍、燻煙処理、発酵処理のほか、飲食物中に抗菌剤を添加する方法が行われてきており、その抗菌成分として天然物由来の抗菌剤が注目されている。しかしながら、イチゴ果の搾汁や極性溶媒抽出エキスを静菌ないし抗菌活性成分として使用することについては、いずれの文献にも記載も示唆もされていない。 On the other hand, food preservatives or food additives that use antibacterial activity, or antibacterial compositions formulated in cosmetics for the purpose of prevention and treatment of acne, oral hygiene for the purpose of prevention and treatment of dental caries and periodontal disease Various attempts have been made to use herbal medicines that are safer than conventional antibacterial compositions and deodorants and deodorants that use antibacterial activity. For example, as a method for preserving food and drink for a long period of time, in addition to heating, drying, salt storage, freezing, smoke treatment, and fermentation treatment, methods for adding an antibacterial agent to food and drink have been performed. As an antibacterial component, antibacterial agents derived from natural products have attracted attention. However, there is no description or suggestion about the use of strawberry fruit juice or polar solvent extract as a bacteriostatic or antibacterial active ingredient.
従来より、飲食物の保存剤ないし日持向上剤、あるいはこの保存剤等としての食品添加物や抗菌剤などの静菌ないし抗菌性成分としては、安全性の高い天然物由来の静菌ないし抗菌性物質、例えばエゴノキ抽出物、カワラヨモギ抽出物、ヒノキチオール、ペクチン分解物、ホオノキ抽出物、レンギョウ抽出物、モウソウチク抽出物、わさび抽出物、茶抽出物、にんにく抽出物、ユッカ抽出物、マンゴー種子核抽出物等々を用いることなど種々検討されてきたが、安価で、安全性、静菌ないし抗菌活性等の点で十分満足できる抗菌組成物はまだ得られていないのが現状であり、特に安全性について十分確立されている食用植物に含まれる静菌ないし抗菌活性物質、静菌ないし抗菌活性を呈するエキス、搾汁等の提供が求められている。 Conventionally, as a bacteriostatic or antibacterial component such as a food preservative or a shelf life improver, or a food additive or antibacterial agent as a preservative, the bacteriostatic or antibacterial derived from a highly safe natural product Sexual substances, such as egonoki extract, kawara mugi extract, hinoki thiol, pectin degradation product, honoki extract, forsythia extract, mushroom extract, wasabi extract, tea extract, garlic extract, yucca extract, mango seed nuclear extract Although various studies have been made, such as the use of materials, etc., an antibacterial composition that is inexpensive and sufficiently satisfactory in terms of safety, bacteriostatic or antibacterial activity, etc. has not yet been obtained. There is a need to provide bacteriostatic or antibacterial active substances, extracts exhibiting bacteriostatic or antibacterial activity, juices and the like contained in well-established edible plants.
本発明者らは、生活習慣病などの予防のために摂取が奨励・推進されている栄養・生理学的に有効な機能を有する果実や野菜の微生物学的安全性の問題について鋭意研究を継続してきたところであるが、今般市販の18種の果実:計114検体、3種のカット果実:9検体、さらに9種の乾燥果実27検体の総計150検体について、その細菌学的汚染実態の検査を行うとともに、滅菌処理、非滅菌処理と各種保存温度との関係で果実に接種の病原性細菌の増殖の程度について研究を行った結果、滅菌リンゴジュースと、滅菌・非滅菌オレンジ・ジュースで25℃、24時間保存で本菌の増殖が認められ、他の食用果実では全く静菌ないしは殺菌あるいは抗菌活性が全く認められなかったにもかかわらず、全く予想外にも滅菌処理したオランダイチゴではその増殖を有意に抑制し、静菌ないしは抗菌効果を発揮している事実を知見した。
従来、滅菌処理した食用果実の静菌ないし抗菌作用について考察されることはなく、イチゴの搾汁あるいはエキス自体が上記のような静菌ないし抗菌活性を有していたことは、滅菌処理した果汁の微生物学的検討を経なければ見出すことができなかった全くの新知見であり、他の果実の搾汁やエキスでは静菌ないし抗菌活性が無かったにもかかわらずイチゴ果汁のみがこれらの活性を呈したことは、全く予想外のことであった。
The present inventors have continued intensive research on the problem of microbiological safety of fruits and vegetables with nutritionally and physiologically effective functions that are encouraged and promoted for the prevention of lifestyle-related diseases. Actually, the actual bacteriological contamination of 18 kinds of commercially available fruits: a total of 114 specimens, 3 kinds of cut fruit: 9 specimens, and a total of 150 specimens of 9 dried fruit 27 specimens is examined for bacteriological contamination. In addition, as a result of research on the degree of growth of pathogenic bacteria inoculated on the fruit in relation to sterilization treatment, non-sterilization treatment and various storage temperatures, as a result of sterilized apple juice and sterilized non-sterile orange juice at 25 ℃, Oran that has been sterilized completely unexpectedly despite the fact that the growth of the bacterium was observed after storage for 24 hours and no bacteriostatic or bactericidal or antibacterial activity was found in other edible fruits. The strawberries were significantly inhibited their growth was finding the fact that exhibits bacteriostatic or antibacterial effect.
Conventionally, the bacteriostatic or antibacterial action of sterilized edible fruits has never been considered, and strawberry juice or extract itself has the above bacteriostatic or antibacterial activity. This is a completely new finding that could not be found without the microbiological examination of strawberry, and only strawberry juice has these activities even though other fruit juices and extracts have no bacteriostatic or antibacterial activity. It was completely unexpected that
本発明はこれらの知見に基づいて完成されたものであり、
(1)バラ科オランダイチゴ属植物の、イチゴ果の搾汁、または極性溶媒抽出エキスと、担体とを含有する抗菌組成物、
(2)バラ科オランダイチゴ属植物の、イチゴ果の搾汁または極性溶媒抽出エキスが、無菌化処理されたものである請求項1記載の抗菌組成物、
に関するものである。
なお、本発明にいう「抗菌」とは、静菌ないし抗菌、あるいは殺菌をも包括する概念をいい、抗菌組成物とは静菌ないし抗菌活性を有する物質を有効成分として含有する食品組成物、化粧料組成物、医薬組成物、口腔衛生料組成物、消臭・脱臭剤組成物、その他の用途の組成物を意味する。以下、静菌ないし抗菌作用を単に抗菌作用と、静菌ないし抗菌活性を単に抗菌活性と、静菌ないし抗菌作用物質を有効成分とする組成物を単に抗菌組成物と、静菌剤ないし抗菌剤を単に抗菌剤と、それぞれ略記する場合もある。
The present invention has been completed based on these findings,
(1) An antibacterial composition comprising a strawberry fruit juice or polar solvent extract of a rose genus Strawberry genus plant and a carrier,
(2) The antibacterial composition according to claim 1, wherein the strawberry fruit juice or polar solvent extract of the Rosa family Dutch strawberry genus plant is sterilized.
It is about.
The term “antibacterial” as used in the present invention refers to a concept encompassing bacteriostatic or antibacterial or sterilization, and the antibacterial composition is a food composition containing a substance having bacteriostatic or antibacterial activity as an active ingredient, It means cosmetic compositions, pharmaceutical compositions, oral hygiene compositions, deodorant / deodorant compositions, and compositions for other uses. Hereinafter, a bacteriostatic or antibacterial action is simply an antibacterial action, a bacteriostatic or antibacterial activity is simply an antibacterial activity, and a composition containing an active ingredient of a bacteriostatic or antibacterial substance is simply an antibacterial composition, and a bacteriostatic or antibacterial agent. May be abbreviated simply as an antibacterial agent.
前記のように、バラ科オランダイチゴ属に属する植物のイチゴ果は、ビタミンCやリンゴ酸、クエン酸なども豊富な園芸果実として永い間摂取され、安全性も確立されている。
また、後記する実験例からも明らかなように、本発明は無菌化処理したオランダイチゴのイチゴ果の搾汁、または極性溶媒抽出エキスを用い、かつ高温保存下においてその属性である抗菌活性を初めて見出した点に特徴を有するものである。したがって、本発明によれば、バラ科オランダイチゴ属に属する植物のいわゆるイチゴ果の抗菌活性を示すその搾汁、または極性溶媒抽出エキスと、担体とからなる抗菌組成物を提供することが可能となる。
As described above, strawberry fruits of plants belonging to the genus Rosaceae of the Rosaceae family have been ingested for a long time as horticultural fruits rich in vitamin C, malic acid, citric acid, etc., and safety has been established.
Further, as will be apparent from the experimental examples described below, the present invention is the first to exhibit antibacterial activity, which is an attribute of sterilized Dutch strawberry juice or polar solvent extract and stored at high temperature. It is characterized by the found points. Therefore, according to the present invention, it is possible to provide an antibacterial composition comprising a juice or a polar solvent extract extract exhibiting antibacterial activity of a so-called strawberry fruit of a plant belonging to the genus Rosaceae belonging to the Rosaceae family, and a carrier. Become.
また、本発明によれば、抗菌活性を有するバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスを有効成分とする食品保存剤、食品添加物あるいはこれらの食品保存剤や食品添加物を配合させた飲食物を提供することが可能となる。
さらに本発明によれば、抗菌活性を有するバラ科オランダイチゴ属植物のイチゴ果の搾汁、または極性溶媒抽出エキスを有効成分とするにきび治療用などの化粧料、絆創膏などに添加する抗菌剤、抗う蝕剤、抗歯周病剤などの口中衛生料、消臭剤・脱臭剤等種々の用途の製品を提供することが可能となる。
In addition, according to the present invention, food preservatives, food additives, or food preservatives or food additives containing, as an active ingredient, squeezed strawberry fruit juice or polar solvent extract of Rosaceae, which has antibacterial activity It becomes possible to provide foods and drinks containing foods.
Furthermore, according to the present invention, antibacterial agents added to squeezed strawberry fruits of the Rosaceae genus Strawberry genus plant having antibacterial activity, or cosmetics for treating acne using a polar solvent extract as an active ingredient, adhesive bandages, It becomes possible to provide products for various uses such as oral hygiene agents such as anti-cariogenic agents and anti-periodontal agents, deodorants and deodorants.
また本発明によれば、抗菌作用物質として有用なバラ科オランダイチゴ属植物のイチゴ果の極性溶媒抽出エキス、特に水、低級アルコール、またはこれらの混合溶媒抽出エキス、特には水、メタノール、エタノール、あるいはこれらの混合溶媒抽出エキス自体の提供をも可能にするものである。 Further, according to the present invention, a polar solvent extract of strawberry fruits of Rosaceae, which is useful as an antibacterial agent, particularly water, lower alcohol, or a mixed solvent extract thereof, particularly water, methanol, ethanol, Alternatively, these mixed solvent extract extracts themselves can be provided.
以下、本発明につき詳述する。
本発明に用いられる「バラ科オランダイチゴ属に属する植物」とは、そのイチゴ果(偽果)の搾汁あるいは極性溶媒抽出エキスが抗菌作用を呈する植物であれば特に制限はない。入手の容易さにおいては交配種が優れるが場合によっては原種を使用することも出来る。原種としてはFragaria chiloensis, Fragaria daltoniana, Fragaria gracilis, Fragaria iinumae, Fragaria moschata, Fragaria nilgerrensis, Fragaria nipponica, Fragaria nubicola, Fragaria orientalis, Fragaria pentaphylla, Fragaria vesca, Fragaria virginiana, Fragaria viridis, などが挙げられる。またこれらの原種の亜種や原種間交配種が含まれる。交配種としては日本では農業品種名が普通に用いられ、女峰、とよのか、麗紅、さちのか、とちおとめ、とよひめ等多くが知られている。また、オランダイチゴの近縁属であるRubus属などの類縁植物も挙げられる。特に工業生産性をも考慮するときは、偽果が大きく、収穫量も多く、成長が早く、稔性のよいオランダイチゴ植物が好ましい。
また、本発明の「イチゴ果」は、前記の通り、膨らんだ花床および果実である痩果からなる偽果を意味する。また、特に「イチゴ果」と特定せずに単にイチゴと特定した場合においても、イチゴ果を意味する場合がある。
本発明で使用の「極性溶媒」とは極性を有する溶媒であって、無極性の溶剤とは異なる溶媒を意味し水と相溶性を有する有機溶媒も含まれる。本発明の目的を達成できる極性溶媒であれば特に限定はないが、水、低級アルコール、低級ケトンまたはこれらの混合溶媒が好ましく、さらに好ましくは、水、メタノール、エタノール、イソプロパノール、アセトンまたはこれらの混合溶媒であり、至適には水、メタノール、エタノールまたはアセトンである。
Hereinafter, the present invention will be described in detail.
The “plant belonging to the genus Rosaceae of the Rosaceae family” used in the present invention is not particularly limited as long as the juice of the strawberry fruit (pseudofruit) or the polar solvent extract exhibits an antibacterial action. In terms of availability, hybrid species are excellent, but in some cases, the original species can also be used. Examples of the original species include Fragaria chiloensis, Fragaria daltoniana, Fragaria gracilis, Fragaria iinumae, Fragaria moschata, Fragaria nilgerrensis, Fragaria nipponica, Fragaria nubicola, Fragaria orientalis, Fragaria pentaphylla, Fragaria vesca, Fragaria viviriana, Fragaria viviris Also included are subspecies of these species and hybrids between species. Agricultural varieties are commonly used in Japan as hybrids, and many are known, such as Nyoho, Toyono, Rei, Sachino, Tochiotome, and Toyohime. There are also related plants such as the genus Rubus, which is a related genus of Dutch strawberry. In particular, when considering industrial productivity, a Dutch strawberry plant having a large false fruit, a large yield, a fast growth, and good fertility is preferable.
In addition, the “strawberry fruit” of the present invention means a false fruit composed of a swollen flower bed and a fruit fruit as described above. In particular, even when a strawberry is simply specified without specifying a “strawberry fruit”, it may mean a strawberry fruit.
The “polar solvent” used in the present invention is a solvent having polarity, which means a solvent different from a nonpolar solvent, and includes an organic solvent having compatibility with water. There is no particular limitation as long as it is a polar solvent that can achieve the object of the present invention, but water, lower alcohol, lower ketone, or a mixed solvent thereof is preferable, and water, methanol, ethanol, isopropanol, acetone, or a mixture thereof is more preferable. Solvent, optimally water, methanol, ethanol or acetone.
本発明の特に特徴とするところは、食用の果実では抗菌作用が知られておらず、これまで全く予期されていなかったバラ科オランダイチゴ属に属する植物のイチゴ果の搾汁、または極性溶媒抽出エキスの属性として抗菌作用を見出した点にあり、本発明にはバラ科オランダイチゴ属植物イチゴ果の搾汁そのもの、および無菌化処理した搾汁、更に極性溶媒抽出エキス、特に極性溶媒が水、メタノール、エタノール、イソプロパノール、アセトンまたはこれらの混合溶媒であるバラ科オランダイチゴ属植物イチゴ果の極性溶媒抽出エキスも包含される。そのような搾汁や抽出エキスは以下のように製造される。
搾汁は、オランダイチゴ属植物のイチゴ果を洗浄し、イチゴ果を破砕し、必要に応じて乾燥することにより得られた破砕物を圧搾し、遠心分離や濾過などにより固液分離して調製される。得られた搾汁や破砕物を添加して食品保存剤、食品添加物、あるいはこれらを添加した飲食物、化粧料、医薬、口腔衛生料、消臭・脱臭剤などに調製する場合など、これらの製品を調製する工程においてビタミンC、エリソルビン酸、亜硫酸等の抗酸化剤等の安定化剤を添加することもできるが、前記破砕・圧搾工程において、必要に応じてこれらの安定化剤を添加し、安定性に優れた搾汁や破砕物とすることもできる。
搾汁は必要に応じ、減圧濃縮などにより濃縮することも可能である。
一方、極性溶媒抽出エキスは、オランダイチゴ属植物のイチゴ果を洗浄し、必要なら破砕し、圧搾し、遠心分離や濾過などのより固液分離した後、凍結乾燥などの手段により乾燥したものを原料とし、水、メタノール、エタノール、イソプロパノール、あるいはこれらの混合溶媒を用いて、必要に応じて加温、浸積、圧搾して、有効成分を抽出する。抽出溶媒が水の場合は、イチゴ果に含まれる水分が水であるから、搾汁から直接濃縮して製造することもできる。
アルコール抽出物は、必要により無水硫酸ナトリウムなどで乾燥する。必要ならば再度、アルコールを用いて溶解と蒸発乾固を反復することにより有効成分を分離、精製することができる。更に、用途に応じて抽出溶媒を留去して用途に適する別の有機溶媒系に移すことも出来る。
無菌化処理は、飲食物や医薬品の分野において任意慣用の無菌化処理手段、例えば加熱処理、照射などの物理的な処理、滅菌ガスやアルコール処理などを使用して実施することができる。
The special feature of the present invention is that the antibacterial action is not known in edible fruits, and the squeezed juice of a strawberry fruit of a plant belonging to the genus Rosaceae belonging to the family Rosaceae, which has never been expected so far, or polar solvent extraction In the point of finding the antibacterial action as an attribute of the extract, in the present invention, the squeezed juice of the Rosaceae genus Strawberry genus plant, and the sterilized juice, and the polar solvent extract, particularly the polar solvent is water, Also included are polar solvent extract extracts of strawberry fruits of the Rosaceae family, which are methanol, ethanol, isopropanol, acetone or mixed solvents thereof. Such juice and extract are produced as follows.
The juice is prepared by washing the strawberry fruit of the Dutch genus plant, crushing the strawberry fruit, pressing the crushed material obtained by drying if necessary, and separating it into solid and liquid by centrifugation, filtration, etc. Is done. When adding the resulting juice or crushed material to prepare food preservatives, food additives, or foods, cosmetics, medicines, oral hygiene, deodorizers, etc. Stabilizers such as antioxidants such as vitamin C, erythorbic acid and sulfurous acid can also be added in the process of preparing the products of the above, but these stabilizers are added as necessary in the crushing and pressing process And it can also be set as the squeezed juice and crushed material excellent in stability.
The squeezed juice can be concentrated by vacuum concentration or the like as necessary.
On the other hand, the polar solvent extract is a strawberry fruit of the Dutch strawberry plant, washed if necessary, crushed, pressed, solid-liquid separated by centrifugation, filtration, etc., and then dried by means such as freeze-drying. The active ingredient is extracted by heating, dipping, and pressing as necessary using water, methanol, ethanol, isopropanol, or a mixed solvent thereof as a raw material. When the extraction solvent is water, the water contained in the strawberry fruit is water, and therefore, it can be produced by concentrating directly from the juice.
The alcohol extract is dried with anhydrous sodium sulfate or the like if necessary. If necessary, the active ingredient can be separated and purified again by repeating dissolution and evaporation to dryness using alcohol. Furthermore, the extraction solvent can be distilled off according to the use and transferred to another organic solvent system suitable for the use.
The sterilization treatment can be carried out using any conventional sterilization treatment means in the field of foods and beverages and pharmaceuticals, for example, physical treatment such as heat treatment and irradiation, sterilization gas and alcohol treatment.
このようにして得られた本発明の抗菌活性を有する抽出エキスはそのまま用いることもできるが、必要に応じて、濃縮処理、あるいは抗菌活性の強力な分子量種のものを分画・採取し、精製したものを使用することもできる。
また、抗菌活性を有する搾汁やエキスより、抗菌活性を有する化学物質を単離・精製し、その化学物質を本発明の抗菌組成物の有効成分とすることもできる。
本発明によって得られたバラ科オランダイチゴ属植物イチゴ果の極性溶媒抽出エキス、特に無菌化処理したバラ科オランダイチゴ属植物イチゴ果の極性溶媒抽出エキス、あるいは抗菌活性を有するバラ科オランダイチゴ属植物イチゴ果の搾汁は、担体との組成物として、前記した食品保存剤、食品添加物、これらを配合した飲食物など抗菌活性を利用する種々の製品とすることができる。
The extract having antibacterial activity of the present invention thus obtained can be used as it is, but if necessary, it is concentrated, or fractionated and collected from those having a strong molecular weight with antibacterial activity, and purified. You can also use what you did.
In addition, a chemical substance having antibacterial activity can be isolated and purified from a juice or extract having antibacterial activity, and the chemical substance can be used as an active ingredient of the antibacterial composition of the present invention.
Polar solvent extract of the strawberry fruit of the Rosaceae family, which is obtained by the present invention, in particular, the polar solvent extract of the strawberry fruit of the Rosaceae family, which has been sterilized, or the Dutch strawberry family of antibacterial activity The juice of strawberry fruit can be made into various products utilizing antibacterial activity such as the aforementioned food preservatives, food additives, foods and drinks containing these as compositions with the carrier.
本発明の抗菌組成物の組成成分である「担体」とは、その抗菌組成物が食品保存剤、食品添加物であれば、食品保存剤や食品添加物として許容される担体であり、医薬組成物であれば製薬学的に許容される担体を意味する。以下抗菌組成物として、特に食品保存剤、食品添加物、飲食物、あるいは医薬としての組成物について詳述する。 The “carrier” as a composition component of the antibacterial composition of the present invention is a carrier that is acceptable as a food preservative or food additive if the antibacterial composition is a food preservative or food additive, and has a pharmaceutical composition. If it is a product, it means a pharmaceutically acceptable carrier. Hereinafter, the composition as a food preservative, food additive, food or drink, or medicine will be described in detail as the antibacterial composition.
本発明においてバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスを配合する食品材料は、本発明の目的を達成する限り限定されるものではないが、本発明の搾汁や極性溶媒抽出エキスを添加することにより安全性が高い、食品保存効果を発揮できる種々の飲食物であれば特に制限はないが、特に好ましくはイチゴ風味を有するジュース、ゼリー、ヨーグルト、ムース等のデザート類や、コロッケ、ハンバーグ等加熱後に常温で保存する調理済み食品、パン、うどん、そば、ピザクラフト等のボイル食品、ジャム類、クッキー類、菓子類、乳製品、ドリンク類、みそ等の調味製品等の種々の飲食物とすることが可能である。また、バラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスを、食品保存剤、食品添加物の製品形態とし、種々の飲食物に配合するための製品とすることも可能である。 In the present invention, the food material blended with strawberry fruit juice or polar solvent extract of the Rosaceae genus Strawberry is not limited as long as the object of the present invention is achieved, but the juice or polarity of the present invention There is no particular limitation as long as it is a variety of foods and drinks that are highly safe and can exhibit food preservation effects by adding a solvent extract, but desserts such as juice, jelly, yogurt, mousse and the like having a strawberry flavor are particularly preferred Cooked foods such as croquettes and hamburgers that are stored at room temperature after heating, boiled foods such as bread, udon, soba and pizza craft, jams, cookies, confectionery, dairy products, drinks, miso and other seasoning products It is possible to make various foods and drinks. In addition, squeezed strawberry fruit juice or polar solvent extract of the Rosa genus Strawberry genus plant can be made into product forms of food preservatives and food additives, and can be made into products for blending with various foods and drinks. .
これらの食品材料に添加される食品材料としては、その食品に必須の食品材料の他、蔗糖、ブドウ糖、果糖、麦芽糖、ブドウ糖果糖液糖等の糖類、ゴマ油、トウモロコシ油、大豆油、綿実油、ナタネ油、オリーブ油、ヤシ油等の植物油、高級脂肪酸トリグリセリド、中鎖脂肪酸モノグリセリド、中鎖脂肪酸トリグリセリド等の脂質、ゼラチン等の蛋白質、ポリオキシエチレン硬化ひまし油、ポリオキシエチレンソルビタン高級脂肪酸エステル等の界面活性剤などが挙げられる。
また、食品保存剤、食品添加物、あるいはこれらを配合した飲食物には、抗菌活性について相加的、あるいは相乗的に日持ち向上効果を発揮させるために、さらに一般に食品保存剤としてソルビン酸またはその塩類、ビタミンC、亜硫酸またはその塩類や、メチルパラベンなどの安息香酸系食品保存料のほか、これまで天然物由来の抗菌性物質として検討されてきた、例えばエゴノキ抽出物、カワラヨモギ抽出物、ヒノキチオール、ペクチン分解物、ホオノキ抽出物、レンギョウ抽出物、モウソウチク抽出物、わさび抽出物、茶抽出物、にんにく抽出物、ユッカ抽出物、マンゴー種子核抽出物等を併用することもできる。
これらのバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスは配合される食品保存剤、食品添加物、飲食物等における配合量が摂取される製品の形態によっても異なり、一概に規定できないが飲食物に添加される場合は製品に対し0.01乃至10重量%、好ましくは0.1乃至5重量%の配合割合で配合され、食品保存剤、食品添加剤の抗菌組成物とする場合は、0.1乃至99重量%、好ましくは1乃至80重量%の配合割合で配合される。
これらの食品保存剤、食品添加物を飲食物に添加することにより、飲食品中の一般細菌数の増殖を有効に抑制することが可能であり、飲食物の日持ち向上剤としての機能を発揮する。
The food materials added to these food materials include those essential to the food, as well as sugars such as sucrose, glucose, fructose, maltose and glucose fructose liquid sugar, sesame oil, corn oil, soybean oil, cottonseed oil, rapeseed Surfactants such as oils, vegetable oils such as olive oil, palm oil, lipids such as higher fatty acid triglycerides, medium chain fatty acid monoglycerides, medium chain fatty acid triglycerides, proteins such as gelatin, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan higher fatty acid esters Etc.
In addition, food preservatives, food additives, or foods and drinks containing these additives are generally used as a food preservative or sorbic acid or the like in order to exert an additive or synergistic improvement in antibacterial activity. In addition to benzoic acid food preservatives such as salts, vitamin C, sulfurous acid or its salts, methylparaben, etc., it has been studied as an antibacterial substance derived from natural products so far, for example, Echinoki extract, Kawaramugi extract, Hinokithiol, Pectin A decomposed product, a honoki extract, a forsythia extract, a mulberry extract, a wasabi extract, a tea extract, a garlic extract, a yucca extract, a mango seed core extract and the like can also be used in combination.
The squeezed fruit juice or polar solvent extract of these Rosaceae genus Strawberry genus plants varies depending on the form of the product ingested, such as food preservatives, food additives, food and drink, etc. Although not specified, when added to foods and drinks, it is added in an amount of 0.01 to 10% by weight, preferably 0.1 to 5% by weight, based on the product, and is an antibacterial composition for food preservatives and food additives. In this case, it is blended at a blending ratio of 0.1 to 99% by weight, preferably 1 to 80% by weight.
By adding these food preservatives and food additives to foods and drinks, it is possible to effectively suppress the growth of the number of general bacteria in foods and drinks, and to function as a shelf life improver for foods and drinks .
抗菌組成物が医薬組成物である場合に、抗菌活性を有するバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスに添加される「担体」としての賦形剤その他の添加剤としては、例えば乳糖、マンニトール、ブドウ糖、コーンスターチ、微結晶セルロース等の賦形剤、HPMC、PVP、MC、アラビアゴム等の結合剤、カルメロースカルシウム、L−HPC等の崩壊剤、クエン酸、酒石酸等の酸味料、重炭酸ナトリウムと酸からなる発泡剤、ステビア、アスパルテーム等の人工甘味料、ステアリン酸ナトリウム等の滑沢剤、酸化チタン、酸化亜鉛、赤色あるいは黄色三二酸化鉄等の着色剤、その他香料、防腐剤などが使用される。
なお、これらの医薬賦形剤やその他の添加剤としては、1種または2種以上組合せて添加することができる。また、これらの医薬賦形剤やその他の添加剤の配合量は製造される剤形によって相違し、一概に規定することはできないが、およそ10乃至99.99重量%、好ましくは50乃至99.9重量の割合で配合される。
When the antibacterial composition is a pharmaceutical composition, as an excipient or other additive as a “carrier” to be added to the squeezed fruit juice or polar solvent extract of the Rosaceae genus Strawberry having antibacterial activity Are, for example, excipients such as lactose, mannitol, glucose, corn starch, microcrystalline cellulose, binders such as HPMC, PVP, MC, gum arabic, disintegrants such as carmellose calcium, L-HPC, citric acid, tartaric acid, etc. Acidulants, foaming agents consisting of sodium bicarbonate and acid, artificial sweeteners such as stevia and aspartame, lubricants such as sodium stearate, colorants such as titanium oxide, zinc oxide, red or yellow iron sesquioxide, and others Perfumes and preservatives are used.
These pharmaceutical excipients and other additives can be added alone or in combination of two or more. Further, the blending amounts of these pharmaceutical excipients and other additives differ depending on the dosage form to be produced, and cannot be generally specified, but are approximately 10 to 99.99% by weight, preferably 50 to 99.99%. It is blended at a ratio of 9%.
また、本発明抗菌組成物の有効成分となるバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスは、例えば救急用絆創膏のガーゼに含浸、させて創傷部の細菌汚染の防止、創傷部の治癒促進とすることができるほか、マスクに含浸させて細菌性、ウィルス性疾患から予防、あるいは細菌の増殖を抑制することができる。 In addition, squeezed or polar solvent extract of strawberry fruit of the Rosaceae genus Strawberry genus plant as an active ingredient of the antibacterial composition of the present invention is impregnated in gauze for emergency bandage, for example, to prevent bacterial contamination of the wound part, In addition to promoting healing of wounds, it can be impregnated into a mask to prevent bacterial or viral diseases, or to suppress bacterial growth.
本発明のバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスが有する特異的な静菌ないし抗菌活性は、その適用を食品由来、口臭等の悪臭に対する消臭・脱臭を目的とする消臭剤・脱臭剤、にきび治療を目的とする化粧料、う蝕菌、歯周病菌の増殖を抑制する抗う蝕剤、抗歯周病剤としての有用性も期待されている。これらのバラ科オランダイチゴ属植物のイチゴ果の搾汁もしくは極性溶媒抽出エキスを配合した消臭剤、抗う蝕剤等についても当該分野において通常用いられる調製方法を適用することにより容易に製造することが可能である。 The specific bacteriostatic or antibacterial activity of the squeezed strawberry fruit juice or polar solvent extract of the Rosaceae genus Strawberry plant of the present invention is intended for deodorization and deodorization against bad odors such as food origin and bad breath It is also expected to be useful as a deodorant / deodorant, a cosmetic for the treatment of acne, a caries bacterium, an anti-cariogenic agent that suppresses the growth of periodontal disease bacteria, and an anti-periodontal disease agent. Deodorants, anti-cariogenic agents, etc. containing squeezed strawberry fruit juice or polar solvent extract of these Rosaceae genus Strawberry plants can also be easily manufactured by applying the preparation methods usually used in the field Is possible.
以下に、本発明の実施例を掲記し本発明をさらに詳細に述べるが、本発明がこれらの実施例によって限定されるべきでないことは勿論である。 In the following, examples of the present invention will be listed and the present invention will be described in more detail, but it is needless to say that the present invention should not be limited by these examples.
(1)試料の作成
オランダイチゴ偽果(以下単にイチゴ果と称す)の果汁を用いてその抗菌効果を検討した。イチゴ果100gを精製水で良く洗浄し、家庭用ミキサーを用いて破砕したのち遠心分離器で固液を分離しイチゴジュースを作成した。作成したイチゴジュースに精製水を加え30%の希釈ジュースとした。この希釈ジュースは滅菌処理を施したものと施さないものとに分けて実験試料とした。
(2)試験に供した菌株とその培地(表1)
滅菌、非滅菌の実験試料に表1に示した菌株をそれぞれ接種し5、10、25、及び32℃の環境下に保持し、0、6、24、48、及び72時間後の菌数を測定した。結果を表2に示す。
なお、非滅菌処理で、データにバラツキが見られるのは、最初から雑菌が存在し、これらが影響を与えていることと同時に、これらの雑菌も測定されるためである。
(比較例1)
実施例1と全く同様に、実験をイチゴジュースに代えてオレンジジュースとリンゴジュースについて実施した結果、これらの果実には殺菌効果が認められなかった。
Bacillus cereus菌の様に、菌自体が弱いため時間の経過により自然消滅したケースも認められた。結果を表3と表4に示す。
結果を実施例により詳しく説明する。
(1) Preparation of sample The antibacterial effect was examined using the fruit juice of Dutch strawberry false fruit (henceforth only strawberry fruit). 100 g of strawberry fruits were thoroughly washed with purified water, crushed using a home mixer, and then separated into solid and liquid with a centrifuge to prepare strawberry juice. Purified water was added to the prepared strawberry juice to obtain a diluted juice of 30%. This diluted juice was divided into a sterilized and non-sterilized sample as experimental samples.
(2) Strains used for the test and their media (Table 1)
The reason why the data show a variation in the non-sterilization treatment is that germs are present from the beginning, and these germs are measured, and at the same time, these germs are also measured.
(Comparative Example 1)
Exactly as in Example 1, the experiment was conducted on orange juice and apple juice instead of strawberry juice. As a result, no bactericidal effect was observed on these fruits.
Some cases, like Bacillus cereus, disappeared spontaneously over time due to the weakness of the bacterium itself. The results are shown in Tables 3 and 4.
The results will be described in detail with reference to examples.
イチゴ果の水抽出エキスの製造
イチゴ果100gを精製水で良く洗浄し、家庭用ミキサーで破砕したのち、凍結乾燥機にて乾燥し、5gの乾燥固化物を得た。この乾燥固形物をナス型フラスコに移し、精製水100mlを添加し、80℃に加温しながら超音波振動装置で10分間超音波振動を加えて有効成分を抽出した。更に遠心分離により固液を分離したのち、80℃に加温して余分な水分を留去した。最終的に6mlの水抽出エキスを得た。
Manufacture of water extract of strawberry fruit 100 g of strawberry fruit was washed well with purified water, crushed with a home mixer, and then dried with a freeze dryer to obtain 5 g of dried solidified product. The dried solid was transferred to an eggplant-shaped flask, 100 ml of purified water was added, and the active ingredient was extracted by applying ultrasonic vibration for 10 minutes with an ultrasonic vibration device while heating to 80 ° C. Further, the solid-liquid was separated by centrifugation, and then heated to 80 ° C. to distill off excess water. Finally, 6 ml of water extract was obtained.
イチゴ果のメタノール抽出エキスの製造
イチゴ果100gを精製水で良く洗浄し、家庭用ミキサーで破砕したのち、凍結乾燥機にて乾燥し、5gの乾燥固化物を得た。この乾燥固形物をナス型フラスコに移し、メタノール100mlを添加し、60℃に加温しながら超音波振動装置で10分間超音波振動を加えて有効成分を抽出した。更に遠心分離により固液を分離したのち、70℃に加温して余分なメタノールを留去した。最終的に5mlのメタノール抽出エキスを得た。
Production of strawberry fruit methanol extract 100 g of strawberry fruit was thoroughly washed with purified water, crushed with a home mixer, and then dried with a freeze dryer to obtain 5 g of dried solidified product. The dried solid was transferred to an eggplant-shaped flask, 100 ml of methanol was added, and the active ingredient was extracted by applying ultrasonic vibration for 10 minutes with an ultrasonic vibration device while heating to 60 ° C. Further, the solid-liquid was separated by centrifugation, and then heated to 70 ° C. to distill off excess methanol. Finally, 5 ml of methanol extract was obtained.
実施例2および実施例3で製造した濃縮エキスについてディスク法を用いて37検体の細菌に対する作用を半定量的に調べた。結果を表5に示す。結果から明らかなように、これらのエキスは抗菌性を示し、水抽出エキスに比べ、極性溶媒抽出エキスの方がより高い抗菌力を示す。
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Cited By (4)
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JP2009298765A (en) * | 2008-03-14 | 2009-12-24 | Oriza Yuka Kk | Gene expression promoting agent for skin beautifying use, and moisture-retention composition and skin beautifying composition produced by using the same |
JP2010047483A (en) * | 2008-08-19 | 2010-03-04 | Ichimaru Pharcos Co Ltd | Collagen gel shrinkage-promoting agent |
KR20190094009A (en) * | 2018-02-02 | 2019-08-12 | 동의대학교 산학협력단 | Composition for treating oral disease and inhibiting halitosis containing natural extracts and preparation method therefor |
KR20190099782A (en) * | 2018-02-20 | 2019-08-28 | 충남대학교산학협력단 | Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component |
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JPH04237456A (en) * | 1991-01-22 | 1992-08-25 | Morinaga & Co Ltd | Preparation of food utilizing raw fruit |
JPH099952A (en) * | 1995-06-27 | 1997-01-14 | Takako Momose | Medical liquor |
JPH111429A (en) * | 1997-06-11 | 1999-01-06 | Lotte Co Ltd | Anti-helicobacter pylori and food and drink for anti-gastritis, and gastric and duodenal ulcer, containing the same |
JPH1143442A (en) * | 1997-07-25 | 1999-02-16 | Lotte Co Ltd | Legionella bacteria resistant composition |
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JPH04237456A (en) * | 1991-01-22 | 1992-08-25 | Morinaga & Co Ltd | Preparation of food utilizing raw fruit |
JPH099952A (en) * | 1995-06-27 | 1997-01-14 | Takako Momose | Medical liquor |
JPH111429A (en) * | 1997-06-11 | 1999-01-06 | Lotte Co Ltd | Anti-helicobacter pylori and food and drink for anti-gastritis, and gastric and duodenal ulcer, containing the same |
JPH1143442A (en) * | 1997-07-25 | 1999-02-16 | Lotte Co Ltd | Legionella bacteria resistant composition |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009298765A (en) * | 2008-03-14 | 2009-12-24 | Oriza Yuka Kk | Gene expression promoting agent for skin beautifying use, and moisture-retention composition and skin beautifying composition produced by using the same |
JP2010047483A (en) * | 2008-08-19 | 2010-03-04 | Ichimaru Pharcos Co Ltd | Collagen gel shrinkage-promoting agent |
KR20190094009A (en) * | 2018-02-02 | 2019-08-12 | 동의대학교 산학협력단 | Composition for treating oral disease and inhibiting halitosis containing natural extracts and preparation method therefor |
KR102171798B1 (en) | 2018-02-02 | 2020-10-29 | 동의대학교 산학협력단 | Composition for treating oral disease and inhibiting halitosis containing natural extracts and preparation method therefor |
KR20190099782A (en) * | 2018-02-20 | 2019-08-28 | 충남대학교산학협력단 | Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component |
KR102050305B1 (en) * | 2018-02-20 | 2019-11-29 | 충남대학교 산학협력단 | Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component |
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