JP2005220078A - Antibacterial composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an excellent antibacterial component, an extract, squeezed juice, or the like contained in an edible plant whose safety is sufficiently confirmed and which is conventionally used as a crude drug or served in forms of various foods and beverages. <P>SOLUTION: The subject antibacterial composition contains juice or a polar solvent extract of a fruit of a genus Fragaria plant belonging to the Rosaceae family and a carrier. An antibacterial agent, a deodorant, an anti-caries agent, a functional food, a food additive and food and beverage each contains the juice or the polar solvent extract of the fruit of the Fragaria plant belonging to the Rosaceae family as an active ingredient. The polar solvent extract of the Fragaria plant belonging to the Rosaceae family is also provided. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an antibacterial composition comprising a juice obtained from strawberry fruit or a polar solvent extract and a carrier, and a polar solvent extract extract of strawberry fruit useful as an antibacterial agent.

Strawberries as so-called fruits are strawberry fruits of Dutch strawberry (scientific name: Fragaria × magna Thuill. Or F. chiloensis. (L) Duchen. Var ananasa Bailey, etc.) belonging to the Rosaceae family. This strawberry fruit consists of a swelled flower bed (flower bud) and the fruit berry on it, and is called a false fruit. Dutch strawberries are edible plants that are widely cultivated nowadays because strawberry fruits (pseudofruits) are sweet, fragrant, and large in shape. Dutch strawberries were produced in Europe in the 17th and 18th centuries, and are said to have been brought to Japan by the Dutch during the Edo period Tenpo.
The Dutch strawberry fruit is used mainly as raw edible and also as a raw material for strawberry syrup, strawberry jam and jelly. In addition, since ancient times, research on bioactive substances contained in various natural products, especially plant materials, has been actively conducted. The Dutch strawberry leaves have tannins, flavonoids, strawberry fruits have sugar, malic acid. , Citric acid and the like. It is also well known that strawberry fruits contain a large amount of vitamin C ascorbic acid. It is also known that these strawberry fruits and leaves are used as astringent, antidiuretic, anti-scurvy, tonic, laxative, and strawberry fruits are used as nourishing and refreshing drugs (see, for example, Non-Patent Document 1). .

Patent Document 1 discloses an active oxygen scavenging species containing dopamine, and an active oxygen scavenging material, a filter, a health food, a food, and a beverage containing active oxygen scavenging promoting species that promote the action of the scavenging species. It is described that “strawberry” is included as one of the oxygen-erasing species.
Patent Document 2 discloses a fruit polyphenol obtained by squeezing and / or extracting from an immature fruit belonging to the family Rosaceae and purifying a fraction thereof, a production method thereof, an antioxidant, a blood pressure lowering agent, Inventions such as mutagenic agents, allergic agents, anti-cariogenic agents, and deodorants have been described, and tea polyphenols (catechins) have a wide range of physiological effects such as antibacterial, anti-cariogenic, and deodorant as conventional techniques. Although it is described that it is being recognized that it has an immature fruit of a rose family tree such as apple, pear, peach, etc. in the present invention, examples of strawberry and other specific examples are described. There is no description.
Furthermore, Patent Documents 3 and 4 include peaks obtained when a polyphenol fraction obtained from fruits, fruit seeds, and the like is separated by liquid column chromatography for reverse phase separation, and major peaks among them are obtained. Among them, inventions such as a bacteriostatic agent containing a component corresponding to the peak eluted last as a main component, a beverage containing the bacteriostatic agent, and a method for treating a beverage additive are described. However, only the grape seed extract is described in the examples as the polyphenol-containing fruit and fruit type, and no specific disclosure about strawberry is shown.
Similarly, Patent Document 5 describes a technology that includes fruit polyphenol-containing fruit juice characterized by being extracted and / or extracted from an immature fruit of a fruit belonging to the family Rosaceae, Only the immature fruits of the Rosaceae tree such as apples, pears, peaches, etc. are described, but the physiological action of the strawberry fruits is not specifically described as described above.

  On the other hand, food preservatives or food additives that use antibacterial activity, or antibacterial compositions formulated in cosmetics for the purpose of prevention and treatment of acne, oral hygiene for the purpose of prevention and treatment of dental caries and periodontal disease Various attempts have been made to use herbal medicines that are safer than conventional antibacterial compositions and deodorants and deodorants that use antibacterial activity. For example, as a method for preserving food and drink for a long period of time, in addition to heating, drying, salt storage, freezing, smoke treatment, and fermentation treatment, methods for adding an antibacterial agent to food and drink have been performed. As an antibacterial component, antibacterial agents derived from natural products have attracted attention. However, there is no description or suggestion about the use of strawberry fruit juice or polar solvent extract as a bacteriostatic or antibacterial active ingredient.

Primary color Makino Wakahan medicinal herb encyclopedia, October 31, 1988, published by Hokuryukan Co., Ltd. JP 2001-158742 A JP-A-7-285876 JP 2001-220307 A JP-A-8-275633 JP-A-8-259453

  Conventionally, as a bacteriostatic or antibacterial component such as a food preservative or a shelf life improver, or a food additive or antibacterial agent as a preservative, the bacteriostatic or antibacterial derived from a highly safe natural product Sexual substances, such as egonoki extract, kawara mugi extract, hinoki thiol, pectin degradation product, honoki extract, forsythia extract, mushroom extract, wasabi extract, tea extract, garlic extract, yucca extract, mango seed nuclear extract Although various studies have been made, such as the use of materials, etc., an antibacterial composition that is inexpensive and sufficiently satisfactory in terms of safety, bacteriostatic or antibacterial activity, etc. has not yet been obtained. There is a need to provide bacteriostatic or antibacterial active substances, extracts exhibiting bacteriostatic or antibacterial activity, juices and the like contained in well-established edible plants.

The present inventors have continued intensive research on the problem of microbiological safety of fruits and vegetables with nutritionally and physiologically effective functions that are encouraged and promoted for the prevention of lifestyle-related diseases. Actually, the actual bacteriological contamination of 18 kinds of commercially available fruits: a total of 114 specimens, 3 kinds of cut fruit: 9 specimens, and a total of 150 specimens of 9 dried fruit 27 specimens is examined for bacteriological contamination. In addition, as a result of research on the degree of growth of pathogenic bacteria inoculated on the fruit in relation to sterilization treatment, non-sterilization treatment and various storage temperatures, as a result of sterilized apple juice and sterilized non-sterile orange juice at 25 ℃, Oran that has been sterilized completely unexpectedly despite the fact that the growth of the bacterium was observed after storage for 24 hours and no bacteriostatic or bactericidal or antibacterial activity was found in other edible fruits. The strawberries were significantly inhibited their growth was finding the fact that exhibits bacteriostatic or antibacterial effect.
Conventionally, the bacteriostatic or antibacterial action of sterilized edible fruits has never been considered, and strawberry juice or extract itself has the above bacteriostatic or antibacterial activity. This is a completely new finding that could not be found without the microbiological examination of strawberry, and only strawberry juice has these activities even though other fruit juices and extracts have no bacteriostatic or antibacterial activity. It was completely unexpected that

The present invention has been completed based on these findings,
(1) An antibacterial composition comprising a strawberry fruit juice or polar solvent extract of a rose genus Strawberry genus plant and a carrier,
(2) The antibacterial composition according to claim 1, wherein the strawberry fruit juice or polar solvent extract of the Rosa family Dutch strawberry genus plant is sterilized.
It is about.
The term “antibacterial” as used in the present invention refers to a concept encompassing bacteriostatic or antibacterial or sterilization, and the antibacterial composition is a food composition containing a substance having bacteriostatic or antibacterial activity as an active ingredient, It means cosmetic compositions, pharmaceutical compositions, oral hygiene compositions, deodorant / deodorant compositions, and compositions for other uses. Hereinafter, a bacteriostatic or antibacterial action is simply an antibacterial action, a bacteriostatic or antibacterial activity is simply an antibacterial activity, and a composition containing an active ingredient of a bacteriostatic or antibacterial substance is simply an antibacterial composition, and a bacteriostatic or antibacterial agent. May be abbreviated simply as an antibacterial agent.

As described above, strawberry fruits of plants belonging to the genus Rosaceae of the Rosaceae family have been ingested for a long time as horticultural fruits rich in vitamin C, malic acid, citric acid, etc., and safety has been established.
Further, as will be apparent from the experimental examples described below, the present invention is the first to exhibit antibacterial activity, which is an attribute of sterilized Dutch strawberry juice or polar solvent extract and stored at high temperature. It is characterized by the found points. Therefore, according to the present invention, it is possible to provide an antibacterial composition comprising a juice or a polar solvent extract extract exhibiting antibacterial activity of a so-called strawberry fruit of a plant belonging to the genus Rosaceae belonging to the Rosaceae family, and a carrier. Become.

In addition, according to the present invention, food preservatives, food additives, or food preservatives or food additives containing, as an active ingredient, squeezed strawberry fruit juice or polar solvent extract of Rosaceae, which has antibacterial activity It becomes possible to provide foods and drinks containing foods.
Furthermore, according to the present invention, antibacterial agents added to squeezed strawberry fruits of the Rosaceae genus Strawberry genus plant having antibacterial activity, or cosmetics for treating acne using a polar solvent extract as an active ingredient, adhesive bandages, It becomes possible to provide products for various uses such as oral hygiene agents such as anti-cariogenic agents and anti-periodontal agents, deodorants and deodorants.

  Further, according to the present invention, a polar solvent extract of strawberry fruits of Rosaceae, which is useful as an antibacterial agent, particularly water, lower alcohol, or a mixed solvent extract thereof, particularly water, methanol, ethanol, Alternatively, these mixed solvent extract extracts themselves can be provided.

Hereinafter, the present invention will be described in detail.
The “plant belonging to the genus Rosaceae of the Rosaceae family” used in the present invention is not particularly limited as long as the juice of the strawberry fruit (pseudofruit) or the polar solvent extract exhibits an antibacterial action. In terms of availability, hybrid species are excellent, but in some cases, the original species can also be used. Examples of the original species include Fragaria chiloensis, Fragaria daltoniana, Fragaria gracilis, Fragaria iinumae, Fragaria moschata, Fragaria nilgerrensis, Fragaria nipponica, Fragaria nubicola, Fragaria orientalis, Fragaria pentaphylla, Fragaria vesca, Fragaria viviriana, Fragaria viviris Also included are subspecies of these species and hybrids between species. Agricultural varieties are commonly used in Japan as hybrids, and many are known, such as Nyoho, Toyono, Rei, Sachino, Tochiotome, and Toyohime. There are also related plants such as the genus Rubus, which is a related genus of Dutch strawberry. In particular, when considering industrial productivity, a Dutch strawberry plant having a large false fruit, a large yield, a fast growth, and good fertility is preferable.
In addition, the “strawberry fruit” of the present invention means a false fruit composed of a swollen flower bed and a fruit fruit as described above. In particular, even when a strawberry is simply specified without specifying a “strawberry fruit”, it may mean a strawberry fruit.
The “polar solvent” used in the present invention is a solvent having polarity, which means a solvent different from a nonpolar solvent, and includes an organic solvent having compatibility with water. There is no particular limitation as long as it is a polar solvent that can achieve the object of the present invention, but water, lower alcohol, lower ketone, or a mixed solvent thereof is preferable, and water, methanol, ethanol, isopropanol, acetone, or a mixture thereof is more preferable. Solvent, optimally water, methanol, ethanol or acetone.

The special feature of the present invention is that the antibacterial action is not known in edible fruits, and the squeezed juice of a strawberry fruit of a plant belonging to the genus Rosaceae belonging to the family Rosaceae, which has never been expected so far, or polar solvent extraction In the point of finding the antibacterial action as an attribute of the extract, in the present invention, the squeezed juice of the Rosaceae genus Strawberry genus plant, and the sterilized juice, and the polar solvent extract, particularly the polar solvent is water, Also included are polar solvent extract extracts of strawberry fruits of the Rosaceae family, which are methanol, ethanol, isopropanol, acetone or mixed solvents thereof. Such juice and extract are produced as follows.
The juice is prepared by washing the strawberry fruit of the Dutch genus plant, crushing the strawberry fruit, pressing the crushed material obtained by drying if necessary, and separating it into solid and liquid by centrifugation, filtration, etc. Is done. When adding the resulting juice or crushed material to prepare food preservatives, food additives, or foods, cosmetics, medicines, oral hygiene, deodorizers, etc. Stabilizers such as antioxidants such as vitamin C, erythorbic acid and sulfurous acid can also be added in the process of preparing the products of the above, but these stabilizers are added as necessary in the crushing and pressing process And it can also be set as the squeezed juice and crushed material excellent in stability.
The squeezed juice can be concentrated by vacuum concentration or the like as necessary.
On the other hand, the polar solvent extract is a strawberry fruit of the Dutch strawberry plant, washed if necessary, crushed, pressed, solid-liquid separated by centrifugation, filtration, etc., and then dried by means such as freeze-drying. The active ingredient is extracted by heating, dipping, and pressing as necessary using water, methanol, ethanol, isopropanol, or a mixed solvent thereof as a raw material. When the extraction solvent is water, the water contained in the strawberry fruit is water, and therefore, it can be produced by concentrating directly from the juice.
The alcohol extract is dried with anhydrous sodium sulfate or the like if necessary. If necessary, the active ingredient can be separated and purified again by repeating dissolution and evaporation to dryness using alcohol. Furthermore, the extraction solvent can be distilled off according to the use and transferred to another organic solvent system suitable for the use.
The sterilization treatment can be carried out using any conventional sterilization treatment means in the field of foods and beverages and pharmaceuticals, for example, physical treatment such as heat treatment and irradiation, sterilization gas and alcohol treatment.

The extract having antibacterial activity of the present invention thus obtained can be used as it is, but if necessary, it is concentrated, or fractionated and collected from those having a strong molecular weight with antibacterial activity, and purified. You can also use what you did.
In addition, a chemical substance having antibacterial activity can be isolated and purified from a juice or extract having antibacterial activity, and the chemical substance can be used as an active ingredient of the antibacterial composition of the present invention.
Polar solvent extract of the strawberry fruit of the Rosaceae family, which is obtained by the present invention, in particular, the polar solvent extract of the strawberry fruit of the Rosaceae family, which has been sterilized, or the Dutch strawberry family of antibacterial activity The juice of strawberry fruit can be made into various products utilizing antibacterial activity such as the aforementioned food preservatives, food additives, foods and drinks containing these as compositions with the carrier.

  The “carrier” as a composition component of the antibacterial composition of the present invention is a carrier that is acceptable as a food preservative or food additive if the antibacterial composition is a food preservative or food additive, and has a pharmaceutical composition. If it is a product, it means a pharmaceutically acceptable carrier. Hereinafter, the composition as a food preservative, food additive, food or drink, or medicine will be described in detail as the antibacterial composition.

  In the present invention, the food material blended with strawberry fruit juice or polar solvent extract of the Rosaceae genus Strawberry is not limited as long as the object of the present invention is achieved, but the juice or polarity of the present invention There is no particular limitation as long as it is a variety of foods and drinks that are highly safe and can exhibit food preservation effects by adding a solvent extract, but desserts such as juice, jelly, yogurt, mousse and the like having a strawberry flavor are particularly preferred Cooked foods such as croquettes and hamburgers that are stored at room temperature after heating, boiled foods such as bread, udon, soba and pizza craft, jams, cookies, confectionery, dairy products, drinks, miso and other seasoning products It is possible to make various foods and drinks. In addition, squeezed strawberry fruit juice or polar solvent extract of the Rosa genus Strawberry genus plant can be made into product forms of food preservatives and food additives, and can be made into products for blending with various foods and drinks. .

The food materials added to these food materials include those essential to the food, as well as sugars such as sucrose, glucose, fructose, maltose and glucose fructose liquid sugar, sesame oil, corn oil, soybean oil, cottonseed oil, rapeseed Surfactants such as oils, vegetable oils such as olive oil, palm oil, lipids such as higher fatty acid triglycerides, medium chain fatty acid monoglycerides, medium chain fatty acid triglycerides, proteins such as gelatin, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan higher fatty acid esters Etc.
In addition, food preservatives, food additives, or foods and drinks containing these additives are generally used as a food preservative or sorbic acid or the like in order to exert an additive or synergistic improvement in antibacterial activity. In addition to benzoic acid food preservatives such as salts, vitamin C, sulfurous acid or its salts, methylparaben, etc., it has been studied as an antibacterial substance derived from natural products so far, for example, Echinoki extract, Kawaramugi extract, Hinokithiol, Pectin A decomposed product, a honoki extract, a forsythia extract, a mulberry extract, a wasabi extract, a tea extract, a garlic extract, a yucca extract, a mango seed core extract and the like can also be used in combination.
The squeezed fruit juice or polar solvent extract of these Rosaceae genus Strawberry genus plants varies depending on the form of the product ingested, such as food preservatives, food additives, food and drink, etc. Although not specified, when added to foods and drinks, it is added in an amount of 0.01 to 10% by weight, preferably 0.1 to 5% by weight, based on the product, and is an antibacterial composition for food preservatives and food additives. In this case, it is blended at a blending ratio of 0.1 to 99% by weight, preferably 1 to 80% by weight.
By adding these food preservatives and food additives to foods and drinks, it is possible to effectively suppress the growth of the number of general bacteria in foods and drinks, and to function as a shelf life improver for foods and drinks .

When the antibacterial composition is a pharmaceutical composition, as an excipient or other additive as a “carrier” to be added to the squeezed fruit juice or polar solvent extract of the Rosaceae genus Strawberry having antibacterial activity Are, for example, excipients such as lactose, mannitol, glucose, corn starch, microcrystalline cellulose, binders such as HPMC, PVP, MC, gum arabic, disintegrants such as carmellose calcium, L-HPC, citric acid, tartaric acid, etc. Acidulants, foaming agents consisting of sodium bicarbonate and acid, artificial sweeteners such as stevia and aspartame, lubricants such as sodium stearate, colorants such as titanium oxide, zinc oxide, red or yellow iron sesquioxide, and others Perfumes and preservatives are used.
These pharmaceutical excipients and other additives can be added alone or in combination of two or more. Further, the blending amounts of these pharmaceutical excipients and other additives differ depending on the dosage form to be produced, and cannot be generally specified, but are approximately 10 to 99.99% by weight, preferably 50 to 99.99%. It is blended at a ratio of 9%.

  In addition, squeezed or polar solvent extract of strawberry fruit of the Rosaceae genus Strawberry genus plant as an active ingredient of the antibacterial composition of the present invention is impregnated in gauze for emergency bandage, for example, to prevent bacterial contamination of the wound part, In addition to promoting healing of wounds, it can be impregnated into a mask to prevent bacterial or viral diseases, or to suppress bacterial growth.

  The specific bacteriostatic or antibacterial activity of the squeezed strawberry fruit juice or polar solvent extract of the Rosaceae genus Strawberry plant of the present invention is intended for deodorization and deodorization against bad odors such as food origin and bad breath It is also expected to be useful as a deodorant / deodorant, a cosmetic for the treatment of acne, a caries bacterium, an anti-cariogenic agent that suppresses the growth of periodontal disease bacteria, and an anti-periodontal disease agent. Deodorants, anti-cariogenic agents, etc. containing squeezed strawberry fruit juice or polar solvent extract of these Rosaceae genus Strawberry plants can also be easily manufactured by applying the preparation methods usually used in the field Is possible.

  In the following, examples of the present invention will be listed and the present invention will be described in more detail, but it is needless to say that the present invention should not be limited by these examples.

（３）実験方法
滅菌、非滅菌の実験試料に表１に示した菌株をそれぞれ接種し５、１０、２５、及び３２℃の環境下に保持し、０、６、２４、４８、及び７２時間後の菌数を測定した。結果を表２に示す。

一方、非滅菌処理でも、抗菌傾向は認められたが、滅菌処理を行って初めてイチゴジュースの抗菌活性が確認された。
なお、非滅菌処理で、データにバラツキが見られるのは、最初から雑菌が存在し、これらが影響を与えていることと同時に、これらの雑菌も測定されるためである。
（比較例１）
実施例１と全く同様に、実験をイチゴジュースに代えてオレンジジュースとリンゴジュースについて実施した結果、これらの果実には殺菌効果が認められなかった。
Bacillus cereus菌の様に、菌自体が弱いため時間の経過により自然消滅したケースも認められた。結果を表３と表４に示す。

表２、３、４の結果から、非滅菌処理の場合は雑菌の影響もあり判断は困難であるが、滅菌イチゴジュースにはオレンジジュースやリンゴジュースに比べ抗菌力で優位性が認められる。この現象を明確にするため、抽出物の実験を実施した。
結果を実施例により詳しく説明する。 (1) Preparation of sample The antibacterial effect was examined using the fruit juice of Dutch strawberry false fruit (henceforth only strawberry fruit). 100 g of strawberry fruits were thoroughly washed with purified water, crushed using a home mixer, and then separated into solid and liquid with a centrifuge to prepare strawberry juice. Purified water was added to the prepared strawberry juice to obtain a diluted juice of 30%. This diluted juice was divided into a sterilized and non-sterilized sample as experimental samples.
(2) Strains used for the test and their media (Table 1)

(3) Experimental method Sterilized and non-sterilized experimental samples were inoculated with the strains shown in Table 1, respectively, and kept in an environment of 5, 10, 25, and 32 ° C. for 0, 6, 24, 48, and 72 hours. The number of later bacteria was measured. The results are shown in Table 2.

On the other hand, although antibacterial tendency was recognized even in non-sterilization treatment, the antibacterial activity of strawberry juice was confirmed only after sterilization treatment.
The reason why the data show a variation in the non-sterilization treatment is that germs are present from the beginning, and these germs are measured, and at the same time, these germs are also measured.
(Comparative Example 1)
Exactly as in Example 1, the experiment was conducted on orange juice and apple juice instead of strawberry juice. As a result, no bactericidal effect was observed on these fruits.
Some cases, like Bacillus cereus, disappeared spontaneously over time due to the weakness of the bacterium itself. The results are shown in Tables 3 and 4.

From the results of Tables 2, 3 and 4, in the case of non-sterilization treatment, judgment is difficult due to the influence of various bacteria, but sterilized strawberry juice has superior antibacterial activity compared to orange juice and apple juice. In order to clarify this phenomenon, an experiment of an extract was performed.
The results will be described in detail with reference to examples.

Manufacture of water extract of strawberry fruit 100 g of strawberry fruit was washed well with purified water, crushed with a home mixer, and then dried with a freeze dryer to obtain 5 g of dried solidified product. The dried solid was transferred to an eggplant-shaped flask, 100 ml of purified water was added, and the active ingredient was extracted by applying ultrasonic vibration for 10 minutes with an ultrasonic vibration device while heating to 80 ° C. Further, the solid-liquid was separated by centrifugation, and then heated to 80 ° C. to distill off excess water. Finally, 6 ml of water extract was obtained.

Production of strawberry fruit methanol extract 100 g of strawberry fruit was thoroughly washed with purified water, crushed with a home mixer, and then dried with a freeze dryer to obtain 5 g of dried solidified product. The dried solid was transferred to an eggplant-shaped flask, 100 ml of methanol was added, and the active ingredient was extracted by applying ultrasonic vibration for 10 minutes with an ultrasonic vibration device while heating to 60 ° C. Further, the solid-liquid was separated by centrifugation, and then heated to 70 ° C. to distill off excess methanol. Finally, 5 ml of methanol extract was obtained.

About the concentrated extract manufactured in Example 2 and Example 3, the effect | action with respect to bacteria of 37 samples was investigated semiquantitatively using the disk method. The results are shown in Table 5. As is clear from the results, these extracts exhibit antibacterial properties, and the polar solvent extract extract exhibits higher antibacterial activity than the water extract.

Claims (2)

  An antibacterial composition comprising a strawberry fruit juice or polar solvent extract of a rose genus Strawberry plant and a carrier.   The antibacterial composition according to claim 1, wherein a strawberry fruit juice or a polar solvent extract of a rose genus Strawberry genus plant is sterilized.