JP2005179243A - Laminin-5 production promotor containing pantothenic acid or its derivative as active ingredient - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a laminin-5 production promotor effective to exalt and activate the ability of epidermal keratinocytes to produce laminin-5. <P>SOLUTION: This laminin-5 production promotor contains a pantothenic acid derivative as an active ingredient. The promotor is useful mainly in the field of cosmetics or dermatology. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to the cosmetics or dermatological technical field, and more specifically to a production promoter for laminin 5.

  Although the skin structure has been studied using a microscope and an electron microscope for a long time, it was not clear what components contributed to maintaining the structure of the skin. However, in 1991, Dr. Burgerson discovered laminin 5 (see, for example, Non-Patent Document 1), and in addition, autoimmune diseases against laminin 5 and the presence of serious diseases lacking laminin 5 in the gene were discovered. (See Non-Patent Documents 2 and 3, respectively.) In this disease, formation of water bubbles was observed at the boundary between the epidermis and dermis, and it became clear that laminin 5 was an essential component for adhesion between the two. Laminin 5 is the main component of a structure composed of various glycoproteins called basement membranes and proteoglycans located at the boundary between the epidermis and dermis. Laminin 5 further has an activity of promoting adhesion of epidermal cells, and functions to directly bind epidermal cells to the basement membrane (see Non-Patent Document 4). In addition, it is known that it is important for epithelial cells to adhere to basement membrane components in maintaining their cell functions (see Non-Patent Document 4).

  From these facts, it is predicted that laminin 5 plays an essential role in maintaining the entire structure of the skin by maintaining a normal basement membrane structure. Specifically, the production of laminin 5 is promoted by repairing structural changes in the basement membrane due to a decrease in the ability to produce laminin 5 (see Non-Patent Document 5) and healing of wounds that destroy the basement membrane structure (non- This contributes to the regulation of epidermal differentiation (see Non-Patent Document 7), which contributes to preventing or healing skin disorders caused by a decrease in the differentiation ability of the epidermis due to a decrease in the ability to produce laminin 5. That is, it is essential for normal skin function expression that the epidermal basal cells are firmly bound to and adhered to the basement membrane.

Accordingly, it has been expected to identify and obtain substances that improve the skin function by inducing the remodeling of the basement membrane structure by promoting the production of laminin 5 for exhibiting the above specific functions.
Rousselle et al. Cell Biol. 114,567,1991 Fine et al. Am. Acad. Dermatol. , 24, 119, (1991) Aberdam et al., Nat. Genet. 6, 299, (1994) Schmidhauser et al., Proc. Natn. Acad. U. S. A. , 87, 9118, (1990) Tsunenaga et al., Matrix Biology 17, 603-613 (1998). Ryan et al. Biol. Chem. 269, 22779-22787 (1994) J. et al. Cell. Biol. 133, 417-430 (1998)

  Therefore, an object of the present invention is to identify a new substance that promotes the production of laminin 5 so as to exert an action such as repair of structural changes of the basement membrane and wound healing that destroys the basement membrane structure. And is on offer.

  The present inventors have searched for a wide range of compounds in order to obtain a substance capable of promoting the production of laminin 5 as described above. As a result, pantothenic acid is mainly included as an active ingredient in hair conditioners and shampoos. Alternatively, it has been found that a salt or derivative thereof can significantly improve the ability to produce laminin 5 in human epidermal keratinocytes.

  In addition, JP 2001-348369 A suggests that a specific pantothenic acid derivative is useful for skin care through activation of fibroblasts (present in the dermis, which is a lower tissue of the basement membrane). . However, since laminin 5 that is of interest to the present inventors is produced not in the dermis but in epidermal keratinocytes, which are upper tissues or organs, skin disorders associated with a decrease in the ability to produce laminin 5 or Diseases can be distinguished from those associated with impaired fibroblast function or when it is desirable to employ different application methods to prevent or cure them (eg, deliver active ingredients to the dermis) There is no need.)

  Accordingly, the present invention relates to pantothenic acid and salts thereof (for example, salts of alkali metals such as sodium and potassium, or alkaline earth metals such as calcium and magnesium, salts of cosmetic or dermatologically acceptable organic bases) and Provided is a laminin 5 production promoter comprising at least one compound selected from the group consisting of pantothenyl ethyl ether as an active ingredient.

  According to the present invention, in a specific embodiment, the laminin 5 production promoter is used exclusively to prevent or treat skin disorders associated with structural changes in the basement membrane.

  According to the invention, in another specific embodiment, the laminin 5 promoter is used exclusively for healing wounds.

  According to the invention, in yet another specific embodiment, the laminin-5 promoter is used exclusively to regulate epidermal differentiation.

  As another invention, pantothenic acid or a salt thereof or pantothenyl ethyl ether is externally applied to human skin in the cosmetic industry as an active ingredient to provide a method for improving the ability to produce laminin 5 in epidermal keratinocytes of the skin. Is done.

  Further, it can be used as a reagent for detecting or screening for the ability to produce laminin 5 in the skin, particularly human epidermal keratinocytes, such as a positive control.

<Specific Embodiments of Invention>
Pantothenic acid or a salt thereof or pantothenyl ethyl ether (corresponding to panthenol ethyl ether) as an active ingredient in the present invention is commercially available and can be used as it is after purification.

  The laminin 5 production-promoting agent of the present invention is formulated in the cosmetic or dermatological field according to the specific purpose of use, and can be in any dosage form that can come into contact with laminin 5 producing epidermal keratinocytes. There can also be. Examples of such dosage forms usually include aqueous solutions, oil solutions, other solutions, emulsions, creams, gels, suspensions, microcapsules, powders, granules, capsules, solid agents, and the like. After preparing these forms by a method known per se, lotions, emulsions, creams, ointments, plasters, haps, aerosols, injections, internal use (tablets, powders, granules, rounds) Suppositories, syrups, lozenges, etc.), suppositories, etc., can be applied to, pasted, sprayed, injected, drunk and inserted into the body. Among these dosage forms, external preparations for skin such as lotion preparations, emulsions, creams, ointments, plasters, haptics, and aerosols are understood as examples of preparations suitable for the purpose of the present invention. The topical skin preparation described here includes pharmaceuticals, quasi-drugs, and cosmetics, and will be used in the same meaning hereinafter.

  The preparations described in the previous section include excipients, fragrances, etc. that are commonly used in preparing these preparations, fats and oils, surfactants, preservatives, sequestering agents, water-soluble polymers, A sticking agent, a powder component, an ultraviolet protective agent, a moisturizer, a medicinal component, an antioxidant, a pH adjuster, a cleaning agent, a drying agent, an emulsifier, and the like can be appropriately blended. When these various components are blended in the skin activator of the present invention, it is possible as long as the intended effects of the present invention are not impaired.

  Examples of the fats and oils include liquid fats and oils, solid fats and oils, waxes, hydrocarbon oils, higher fatty acids, higher alcohols, synthetic ester oils, and silicones.

  That is, liquid oils and fats include avocado oil, camellia oil, evening primrose oil, turtle oil, macadamia nut oil, corn oil, mink oil, olive oil, rapeseed oil, egg yolk oil, sesame oil, persic oil, wheat germ oil, sasanka oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, cinnagari oil, Japanese kiri oil, jojoba oil, germ oil, triglycerin, glyceryl trioctanoate, tri Isopalmitic acid glycerin and the like are solid fats such as cocoa butter, coconut oil, horse tallow, hydrogenated coconut oil, palm oil, beef tallow, sheep fat, hardened beef tallow, palm kernel oil, pork tallow, beef bone fat, mollusc kernel oil , Hardened oil, beef leg fat, owl, hardened castor oil, etc. waxes include beeswax, candelilla wax, cotton wax, carnauba wax, bayberry wax, warts Wax, whale wax, montan wax, nukarou, lanolin, kapok wax, lanolin acetate, liquid lanolin, sugarcane wax, lanolin fatty acid isopropyl, hexyl laurate, reduced lanolin, jojoballow, hard lanolin, shellac wax, POE lanolin alcohol ether, POE lanolin alcohol acetate Examples of hydrocarbon oils such as POE cholesterol ether, lanolin fatty acid polyethylene glycol, POE hydrogenated lanolin alcohol ether, and oils such as liquid paraffin, ozokerite, squalene, pristane, paraffin, ceresin, squalene, petrolatum, microcrystalline wax, etc. .

  Examples of the higher fatty acid include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tolic acid, isostearic acid, linoleic acid, linolenic acid, ethyl alcohol Examples include icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).

  Examples of higher alcohols include linear alcohols such as lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetostearyl alcohol, monostearyl glycerol ether (batyl alcohol), 2-decyltetradecinol, and lanolin. Examples thereof include branched chain alcohols such as alcohol, cholesterol, phytosterol, hexyldodecanol, isostearyl alcohol, and octyldodecanol.

  Synthetic ester oils include isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate Lanolin acetate, isocetyl stearate, isocetyl isostearate, cholesteryl 12-hydroxystearylate, ethylene glycol di-2-ethylhexylate, dipentaerythritol fatty acid ester, monoisostearic acid N-alkyl glycol, dicapric acid neopentyl glycol, malic acid Diisostearyl, di-2-heptylundecanoic acid glycerin, tri-2-ethylhexyl trimethylolpropane, Trimethylolpropane phosphate, pentane erythritol tetra-2-ethylhexylate, glycerin tri-2-ethylhexylate, trimethylolpropane triisostearate, cetyl 2-ethylhexanoate, 2-ethylhexyl palmitate, glyceryl trimyristate, tris -2-Heptylundecanoic acid glyceride, castor oil fatty acid methyl ester, oleic oil, cetostearyl alcohol, acetoglyceride, 2-heptylundecyl palmitate, diisobutyl adipate, N-lauroyl-L-glutamic acid-2-octyldodecyl ester , Di-2-heptylundecyl adipate, ethyl laurate, di-2-ethylhexyl sebacate, 2-hexyldecyl myristate, 2-hexyl palmitate Sill, 2-hexyldecyl adipate, sebacic acid diisopropyl, 2-ethylhexyl succinate, ethyl acetate, butyl acetate, amyl acetate, and triethyl citrate.

  Examples of silicones include chain polysiloxanes such as dimethylpolysiloxane, methylphenylpolysiloxane, and methylhydrogenpolysiloxane; cyclic polysiloxanes such as decamethylpolysiloxane, dodecamethylpolysiloxane, and tetramethyltetrahydrogenpolysiloxane; Examples thereof include silicone resin and silicone rubber forming a dimensional network structure.

  Examples of the surfactant include an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and a nonionic surfactant.

  Examples of the anionic surfactant include soap bases, fatty acid soaps such as sodium laurate and sodium palmitate, higher alkyl sulfates such as sodium lauryl sulfate and potassium lauryl sulfate, POE lauryl sulfate triethanolamine, and POE lauryl sulfate. Alkyl ether sulfates such as sodium, N-acyl sarcosine acid such as sodium lauroyl sarcosine, N-myristoyl-N-methyl taurine sodium, coconut oil fatty acid methyl tauride sodium, lauryl methyl tauride sodium and other higher fatty acid amide sulfonic acids Salts, phosphoric acid ester salts such as sodium POE oleyl ether phosphate, POE stearyl ether phosphoric acid, sodium di-2-ethylhexyl sulfosuccinate, monolauroylmolybdate Sulfosuccinates such as sodium ethanolamide polyoxyethylene sulfosuccinate and sodium lauryl polypropylene glycol sulfosuccinate, sodium linear dodecylbenzene sulfonate, triethanolamine linear dodecyl benzene sulfonate, alkyl benzene sulfonates such as linear dodecyl benzene sulfonic acid, N -Monosodium lauroyl glutamate, disodium N-stearoyl glutamate, N-acyl glutamate such as monosodium N-myristoyl-L-glutamate, higher fatty acid ester sulfates such as hardened coconut oil fatty acid sodium glycerol sulfate, funnel oil, etc. Sulfated oil, POE alkyl ether carboxylic acid, POE alkyl allyl ether carboxylate, α-olefin sulfonate Higher fatty acid ester sulfonates, secondary alcohol sulfates, higher fatty acid alkylolamide sulfates, sodium lauroyl monoethanolamide succinate, ditriethanolamine N-palmitoyl aspartate, sodium caseinate, and the like.

  Examples of cationic surfactants include alkyltrimethylammonium salts such as stearyltrimethylammonium chloride and lauryltrimethylammonium chloride, distearyldimethylammonium dialkyldimethylammonium chloride, and poly (N, N′-dimethyl-3,5-methylene chloride). Piperidinium), alkyl pyridinium salts such as cetyl pyridinium chloride, alkyl quaternary ammonium salts, alkyl dimethyl benzyl ammonium salts, alkyl isoquinolinium salts, dialkyl morpholinium salts, POE alkyl amines, alkyl amine salts, polyamine fatty acid derivatives Amyl alcohol fatty acid derivatives, benzalkonium chloride, benzethonium chloride and the like.

  Examples of amphoteric surfactants include 2-undecyl-N, N, N- (hydroxyethylcarboxymethyl) -2-imidazoline sodium, 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy 2 Imidazoline-based amphoteric surfactants such as sodium salts, betaine-based surfactants such as 2-heptadecyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, alkylbetaines, amide betaines, sulfobetaines Etc.

  Examples of the lipophilic nonionic surfactant include sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, penta-2 -Sorbitan fatty acid esters such as diglycerol sorbitan tetrahexylhexylate, diglycerol sorbitan tetrahexyl-2-ethylhexylate, mono-cotton oil fatty acid glycerin, glyceryl monoerucate, glyceryl sesquioleate, glyceryl monostearate, α, α'-oleic acid pyro Glycerol polyglycerin fatty acids such as glyceryl glutamate, glyceryl monostearate malate, and propylene glycol fatty acids such as propylene glycol monostearate Examples include esters, hydrogenated castor oil derivatives, glycerin alkyl ethers, polyoxyethylene / methylpolysiloxane copolymers, and the like.

  Examples of the hydrophilic nonionic surfactant include POE sorbitan fatty acid esters such as POE sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate, and POE-sorbit monolaur. POE-sorbite monooleate, POE-sorbite pentaoleate, POE sorbite fatty acid esters such as POE-sorbitol monostearate, POE-glycerol monostearate, POE-glycerol monoisostearate, POE-glycerol triiso POE glycerin fatty acid esters such as stearate, POE fatty acid esters such as POE monooleate, POE distearate, POE monodiolate, ethylene glycol stearate POE alkyl ethers such as POE lauryl ether, POE oleyl ether, POE stearyl ether, POE behenyl ether, POE2-octyldodecyl ether, POE cholestanol ether, POE octyl phenyl ether, POE nonyl phenyl ether, POE dinonyl phenyl ether, etc. POE alkylphenyl ethers, pluronic types such as pluronic, POE / POP cetyl ether, POE / POP2-decyltetradecyl ether, POE / POP monobutyl ether, POE / POP hydrogenated lanolin, POE / POP glycerin ether, etc.・ POP alkyl ethers, Tetra POE such as Tetronic, Tetra POP ethylenediamine condensates, POE castor oil, POE cured H POE castor oil hardened castor oil derivatives, POE hardened castor oil monoisostearate, POE hardened castor oil triisostearate, POE hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE hardened castor oil maleic acid, POE POE beeswax / lanolin derivatives such as sorbitol beeswax, coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, alkanolamides such as fatty acid isopropanolamide, POE propylene glycol fatty acid ester, POE alkylamine, POE fatty acid amide, sucrose fatty acid ester, POE Nonylphenyl formaldehyde condensate, alkylethoxydimethylamine oxide, trioleyl phosphoric acid and the like can be mentioned.

  Examples of the preservative include methyl paraben, ethyl paraben, butyl paraben and the like.

  Examples of the sequestering agent include sodium edetate and EDTA.

  Examples of the water-soluble polymer include natural polymers, semi-synthetic polymers, synthetic polymers, and inorganic polymers.

  Natural water-soluble polymers include gum arabic, gum tragacanth, galactan, guar gum, carob gum, caraya gum, carrageenan, tamarind gum, xanthan gum, pectin, agar, quince seed (malmello), alge colloid (gum extract), starch (rice, corn) Potato, wheat), plant polymers such as glycyrrhizic acid, microbial polymers such as xanthan gum, dextran, succinoglucan and pullulan, and animal polymers such as collagen, casein, albumin and gelatin.

  Semi-synthetic water-soluble polymers include starch polymers such as dextrin, carboxymethyl starch, methylhydroxypropyl starch, methylcellulose, nitrocellulose, ethylcellulose, methylhydroxypropylcellulose, hydroxyethylcellulose, cellulose dimethyldialkyl sulfate (12-20). ) Cellulose polymers such as ammonium, hydroxypropylcellulose, sodium carboxymethylcellulose (CMC), crystalline cellulose, cellulose powder, and alginic acid polymers such as sodium alginate and propylene glycol alginate.

  Synthetic water-soluble polymers include vinyl polymers such as polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, and polyoxyethylene polymers such as polyethylene glycol 2000, 4000, and 6000. , Polyoxyethylene polyoxypropylene copolymer polymer, acrylic polymer such as sodium polyacrylate, polyethylene acrylate, and polyacrylamide, polyethyleneimine, and cationic polymer.

  Examples of inorganic water-soluble polymers include bentonite, aluminum magnesium silicate, laponite, hectorite, and anhydrous silicic acid.

  Examples of the powder component include talc, kaolin, mica, sericite (sericite), muscovite, phlogopite, synthetic mica, saucite, biotite, lithia mica, vermiculite, magnesium carbonate, calcium carbonate, aluminum silicate, silica Barium oxide, calcium silicate, magnesium silicate, strontium silicate, metal tungstate, magnesium, silica, zeolite, barium sulfate, calcined calcium sulfate (baked gypsum), calcium phosphate, fluorine apatite, hydroxyapatite, ceramic powder, metal soap (Zinc myristate, calcium palmitate, aluminum stearate), inorganic powder such as boron nitride, polyamide resin powder (nylon powder), polyethylene powder, polymethyl methacrylate powder, polystyrene powder, styrene Copolymer resin powder of rilic acid, benzoguanamine resin powder, polytetrafluoroethylene powder, organic powder such as cellulose powder, inorganic white pigment such as titanium dioxide and zinc oxide, inorganic such as iron oxide (Bengara) and iron titanate Red pigments, inorganic brown pigments such as γ-iron oxide, inorganic yellow pigments such as yellow iron oxide and ocher, inorganic black pigments such as black iron oxide, carbon black, low-order titanium oxide, mango violet, cobalt violet Inorganic purple pigments such as inorganic oxide pigments such as chromium oxide, chromium hydroxide and cobalt titanate, inorganic blue pigments such as ultramarine and bitumen, titanium oxide coated mica, titanium oxide coated bismuth oxychloride, titanium oxide coated talc , Colored titanium oxide coated mica, bismuth oxychloride, pearl pigments such as fish scale foil, aluminum powder, Metal powder pigments such as upper powder, red 201, red 202, red 204, red 205, red 220, red 226, red 228, red 405, orange 203, orange 204, yellow 205 Yellow 401, Blue 404, Red 3, Red 104, Red 106, Red 227, Red 230, Red 401, Red 505, Orange 205, Yellow 4, Yellow 5, Yellow Organic pigments such as zirconium, barium or aluminum lake such as 202, yellow 203, green 3 and blue 1, natural pigments such as chlorophyll, β-carotene, etc., colorants such as titanium yellow, carsamine, safflower red, Etc.

  The UV protection agent includes both an “UV absorber” that is a substance that chemically absorbs UV rays and an “UV blocker” that is a substance that scatters and reflects UV rays by physical action.

  That is, as a long wavelength ultraviolet (UVA) absorber, anthranilic acid ultraviolet absorbers such as methyl anthranilate and homomenthyl-N-acetylanthranilate, such as 2,4-dihydroxybenzophenone, 2,2-dihydroxy-4- Methoxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2,2 ', 4,4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4 '-Methylbenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfone hydrochloride, 4-phenylbenzophenone, 2-ethylhexyl-4'-phenyl-benzophenone-2-carboxylate, 2-hydroxy-4-n-octoxy Benzophenone, Benzophenone ultraviolet absorbers such as -hydroxy-3-carboxybenzophenone, such as 2,2'-hydroxy-5-methylphenylbenzotriazole, 2- (2'-hydroxy-5'-t-octylphenyl) benzotriazole, 2 Examples include benzotriazole ultraviolet absorbers such as-(2'-hydroxy-5'-methylphenyl) benzotriazole, such as dianisoylmethane, 4-methoxy-4'-t-butyldibenzoylmethane, and the like. Among these long wavelength UV absorbers, 4-methoxy-4'-tert-butyldibenzoylmethane, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone derivatives such as 2-hydroxy-4- Methoxybenzophenone-5-sulfonate is a long wavelength ultraviolet absorber excellent in safety and effectiveness, and is preferable.

  Further, as a medium wavelength ultraviolet (UVB) absorber, paraaminobenzoic acid (hereinafter referred to as PABA), PABA monoglycerin ester, N, N-dipropoxy PABA ethyl ester, N, N-diethoxy PABA ethyl ester, N, N- Benzoic acid UV absorbers such as dimethyl PABA ethyl ester, N, N-dimethyl PABA butyl ester, N, N-dimethyl PABA amyl ester, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, amyl salicylate, menthyl Salicylic acid-based UV absorbers such as salicylate, homomenthyl salicylate, octyl salicylate, phenyl salicylate, benzyl salicylate, p-isopropanol phenyl salicylate Octyl cinnamate, ethyl-4-isopropyl cinnamate, methyl-2,5-diisopropyl cinnamate, ethyl-2,4-diisopropyl cinnamate, methyl-2,4-diisopropyl cinnamate, propyl-p-methoxycinnamate, Isopropyl-p-methoxycinnamate, isoamyl-p-methoxycinnamate, octyl-p-methoxycinnamate (2-ethylhexyl-p-methoxycinnamate), 2-ethoxyethyl-p-methoxycinnamate, cyclohexyl-p- Methoxycinnamate, ethyl-α-cyano-β-phenylcinnamate, 2-ethylhexyl-α-cyano-β-phenylcinnamate, glyceryl mono-2-ethylhexanoyl-diparamethoxycinnamate, methoxycinnamate Cinnamic acid UV absorbers such as octyl, 3,4,5-trimethoxycinnamic acid-3-methyl-4- [methylbis (trimethylsiloxy) silyl] butyl, p-dimethoxycinnamic acid monoethyl ester, 3 Camphor derivatives such as-(4'-methylbenzylidene) -d, 1-camphor, 3-benzylidene-d, 1-camphor, 5- (3,3-dimethyl-2-norbornylidene) -3-pentyn-2-one , Urocanic acid, urocanic acid ethyl ester, 2-phenyl-5-methylbenzoxazole, dibenzalazine and the like.

Furthermore, examples of the ultraviolet blocking agent include titanium oxide (TiO ₂ ), talc (MgSiO ₂ ), carmine (FeO ₂ ), bentonite, kaolin, and zinc oxide (ZnO).

  Examples of the humectant include polyethylene glycol, propylene glycol, glycerin, 1,3-butylene glycol, hexylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucoitin sulfate, caronic acid, atelocollagen, cholesteryl-12. -Hydroxy stearate, sodium lactate, bile salt, dl-pyrrolidone carboxylate, short-chain soluble collagen, diglycerin (EO) PO adduct, Izayoi rose extract, yarrow extract, Merrilot extract, etc. it can.

Examples of the medicinal ingredients include whitening agents such as arbutin, vitamin C and derivatives thereof, kojic acid placenta extract, glutathione, and yukinoshita extract, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, zinc oxide, allantoin and other anti-inflammatory agents Agents, royal jelly, photosensitizers, cholesterol derivatives, activators such as calf blood extract, nonyl acid wallenyl amide, nicotinic acid benzyl ester, nicotinic acid β-butoxyethyl ester, capsaicin, gingerone, cantalis tincture, ictamol, caffeine, Tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, cyclandrate, cinnarizine, tolazoline, acetylcholine, verapamil, cephalanthin, γ-oryzanol, etc. Accelerator: Anti-seborrheic agents such as sulfur and thianthol, for various purposes, alum extract, auren extract, sicon extract, peonies extract, assembly extract, birch extract, sage extract, loquat extract , Carrot extract component, aloe extract component, mallow extract component, iris extract component, grape extract component, yokuinin extract component, loofah extract component, lily extract component, saffron extract component, nematode extract component, ginger extract component, hypericum extract component, Ononisu extract, rosemary extract, garlic extract, capsicum extract, citrus unshiu peel, angelica, etc., retinol, vitamin a such as retinol acetate, riboflavin, riboflavin butyrate, vitamin B ₂ such as flavin adenine nucleotide, pyridoxine hydrochloride , Pyridoxy Vitamin _{B 2} such as dioctanoate, L- ascorbic acid, L- ascorbic acid dipalmitate ester, L- ascorbic acid-2-sodium sulfate, L- ascorbic acid phosphoric acid ester, DL-alpha-tocopherol -L- ascorbate Vitamin C such as dipotassium phosphate diester, pantothenic acid such as calcium pantothenate, D-pantothenyl alcohol, pantothenyl ethyl ether, acetyl pantothenyl ethyl ether, vitamin D such as ergocalciferol, cholecalciferol, nicotinic acid Nicotinic acids such as nicotinamide, benzyl nicotinate, α-tocopherol, tocopherol acetate, DL-α-tocopherol nicotinate, DL-α-tocopherol succinate, vitamin E, vitamin P, biochi Vitamins and the like, can be mentioned.

  In addition, about the chemical | medical agent component, it can mix | blend widely in the range by which the effect of this invention is not impaired by the mixing | blending.

  When the preparation of the present invention thus prepared is applied topically to human skin, the pantothenic acid derivative as an active ingredient is 0.0001% by weight or more, preferably 0, per total weight of the preparation or composition. It may be included in an amount of .0005 wt% to 1 wt%. However, for example, taking into account the type or degree of skin disorder, based on the opinions of experts or doctors in the art, it may be dispensed at concentrations other than those mentioned above, and the patient or an individual who requires treatment with such a formulation Can be administered topically continuously or intermittently until the required amount is applied. Since the preparation according to the present invention has the activity of enhancing the ability to produce laminin 5 in human epidermal keratinocytes, it prevents the functional deterioration associated with structural changes of the skin, particularly the basement membrane, and also activates the skin. Can be promoted.

Hereinafter, the present invention will be described more specifically with specific examples.
Measurement test of laminin 5 production ability:
(1) Culture of epidermal keratinocytes Epidermal keratinocytes were isolated from human foreskin and cultured in epidermal keratinocyte growth medium (KGM) having a low calcium concentration. The bovine pituitary extract and EGF were added to this medium. The cells were cultured in KGM until the fourth generation, then the adherent cells were suspended by trypsin-EDTA treatment, and the cell aggregates were removed by filtration to obtain a uniform cell suspension. Cells were collected by centrifugation and resuspended in DMEM-F12 (2: 1) -0.1% BSA at 8 × 10 ⁴ / ml. 0.5 ml of this cell suspension was added to 0.5 ml of the same medium containing an active ingredient (test drug) having a double concentration. The culture was performed at 37 ° C. for 24 hours using a 24-well plate. At the end of the culture, the culture supernatant was transferred to an Eppendorf tube, centrifuged at 10,000 rpm for 5 minutes, and the supernatant was transferred to a new tube and stored at −20 ° C. until the day of laminin 5 measurement. In order to solubilize laminin 5 bound inside the cell and on the culture plastic, Tris-HCl buffer (pH 7.4) containing various surfactants was added to each well and stored overnight at -20 ° C. The next day, it was sonicated and frozen again. The next day, after dissolving again, the mixture was centrifuged at 10,000 rpm for 5 minutes, the supernatant was transferred to a tube, and stored at −20 ° C. until the day of measurement of laminin 5.

(2) Measurement of laminin 5 by sandwich ELISA method Laminin 5 present in the culture supernatant and cell layer was measured by sandwich ELISA method. A monoclonal antibody against the laminin α3 chain of laminin 5, BM165, was bound to a solid layer of a 96-well ELISA plate. In order to sandwich and measure laminin 5, 6F12, which is a monoclonal antibody against laminin β3 chain, was biotinylated (b-6F12) in advance as another type of antibody. In this method, only the heterotrimeric body (α3β3γ2) capable of exhibiting the function is measured, and the heterodimer (β3γ2) is not detected. Samples are added to each well previously filled with 3% gelatin / phosphate buffer solution containing b-6F12. The final dilution ratio in the sample hole was 1/4 for the culture solution and 1/10 for the cell layer. The antigen-antibody reaction was performed at 37 ° C. for 2 hours, washed, an avidin HRP (horseradish peroxidase) solution was added, and the reaction was further continued for 30 minutes to 1 hour. After washing, an ABTS solution as a substrate for HRP was added, and the absorbance at 405 nm was measured using an ELISA plate reader. A calibration curve was prepared in the range of 0 to 40 ng / ml.

  The production amount of laminin 5 was obtained by calculating the sum of the amount released in the medium and the amount remaining in the cell layer, and expressed as a relative value when the drug-free (control) group was taken as 100%.

(3) Results The results are shown in Table 1 below.

  As is clear from Table 1, the pantothenic acid derivative used in the present invention significantly improves the production of laminin 5 in human epidermal keratinocytes.

Percentages [%] in the following examples represent weight / weight% unless otherwise specified.
Example 1 Cream (Prescription)
(%)
Polyoxyethylene (20 mol addition) cetyl alcohol ether 1.0
Methylphenyl polysiloxane (20cs) 2.0
Liquid paraffin 3.0
2-Hydroxy-4-methoxybenzophenone 5.0
Calcium pantothenate 0.1
Propylene glycol 5.0
Glycerin 2.0
Ethyl alcohol 15.0
Carboxyvinyl polymer (trade name: Carbopol 941,
B. F. Goodrich Chemical company) 0.3
Droxypropylcellulose 0.1
2-Aminomethylpropanol 0.1
Preservative Appropriate amount of perfume Appropriate amount of ion-exchanged water Residual Example 2 Cream (prescription) (%)
Cyclomethicone 10.0
Cetyl octanoate 5.0
Squalane 10.0
Meadow Foam Oil 3.0
Tetrahydrotetramethylcyclotetrasiloxane 5.0
Dimethicone polyol 3.0
Quaternium-18 Hectorite 2.0
Edetate 0.1
Polyethylene glycol 6000 1.0
Glycerin 5.0
Dipropylene glycol 5.0
Pantothenyl ethyl ether 0.1
Tranexamic acid 0.5
Magnesium ascorbate phosphate 0.1
Sodium hyaluronate 0.01
L-arginine hydrochloride 0.01
Methylparaben 0.1
Perfume Appropriate amount of ion-exchanged water Residual Example 3 Wrinkle prevention cream (prescription) (%)
Stearic acid 2.0
Stearyl alcohol 7.0
Hydrogenated Lanolin 2.0
Squalane 5.0
2-Octyldodecyl alcohol 6.0
Polyoxyethylene (25 mol) cetyl alcohol ether 3.0
Glycerin monostearate ester 2.0
Propylene glycol 5.0
Calcium pantothenate 0.05
Turmeric ethanol extract 0.05
Sodium bisulfite 0.03
Ethylparaben 0.3
Fragrance Appropriate amount of ion-exchanged water Residual Example 4 Massage cream (Prescription) (%)
Solid paraffin 5.0
Beeswax 10.0
Vaseline 15.0
Liquid paraffin 41.0
Glycerin monostearate ester 2.0
Polyoxyethylene (20 mol) sorbitan monolaurate 2.0
Soap powder 0.1
Borax 0.2
Pantothenyl ethyl ether 0.05
Psychoethanol extract 0.05
Sodium bisulfite 0.03
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Residual Example 5 Cream (Prescription) (%)
Stearic acid 10.0
Stearyl alcohol 4.0
Butyl stearate 8.0
Stearic acid monoglycerin ester 2.0
Vitamin E acetate 0.5
Vitamin A palmitate 0.1
Macadamia nut oil 1.0
Glycerin 4.0
1,2-pentanediol 3.0
Potassium hydride 0.4
Magnesium ascorbate phosphate 0.1
L-arginine hydrochloride 0.01
Edetate trisodium 0.005
Calcium pantothenate 0.5
Preservative Preservative Perfume Fragrance Ion Exchange Water Residual Example 6 Emulsion (Prescription) (%)
Stearic acid 2.5
Vaseline 5.0
Liquid paraffin 10.0
Polyoxyethylene (10 mol) monooleate 2.0
Polyethylene glycol 1500 3.0
Triethanolamine 1.0
Carboxyvinyl polymer (same as above) 0.05
Pantothenyl ethyl ether 0.01
Arbutin 1.0
Seaweed extract 1.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Residual Example 7 Emulsion (prescription) (%)
Squalane 5.0
Oleyl oleate 3.0
Vaseline 2.0
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20EO) 1.2
Evening primrose oil 0.5
1,3-butylene glycol 4.5
Cascon ethanol extract 1.5
Ethanol 3.0
Carboxyvinyl polymer (same as above) 0.2
Sodium hydride 0.1
L-arginine L-aspartate 0.01
Calcium pantothenate 1.5
Erythritol 0.5
Sodium hexametaphosphate 0.05
Preservative Appropriate amount of perfume Appropriate amount of ion-exchanged water Residual Example 8 Gel (prescription) (%)
95% ethyl alcohol 10.0
Dipropylene glycol 15.0
Polyoxyethylene (50 mol) oleyl alcohol ether 2.0
Carboxyvinyl polymer (same as above) 1.0
Xanthan gum 0.01
Caustic soda 0.15
L-Arginine 0.1
Pantothenyl ethyl ether 7.0
Sodium 2-hydroxy-4-methoxybenzophenone sulfonate 0.05
Ethylenediaminetetraacetate, 3 sodium, 2 water 0.05
Methylparaben 0.2
Perfume Appropriate amount of ion-exchanged water Residual Example 9 Cosmetic liquid (prescription) (%)
Ethyl alcohol (95%) 10.0
Polyoxyethylene (20 mol) octyldodecanol 1.0
Pantothenyl ethyl ether 0.1
Winged bean extract 1.5
Methylparaben 0.15
Potassium hydroxide 0.1
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer (same as above) 0.2
Ion-exchange water Residual Example 10 Lotion (Prescription) (%)
Ethanol 5.0
Polyoxyethylene hydrogenated castor oil 2.0
Oleyl alcohol 0.1
2-Ethylhexyl-P-dimethylaminobenzoate 0.18
1,3-butylene glycol 9.5
Glycerin 2.0
Sodium pyrrolidonecarboxylate 0.5
Nicotinamide 0.3
Calcium pantothenate 0.1
Hinogi extract 1.0
β-cyclodextrin 1.0
Erythritol 0.05
Fragrance Appropriate amount of ion-exchanged water Residual Example 11 Pack (Prescription) (%)
Dipropylene glycol 5.0
Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
Pantothenyl ethyl ether 0.001
Olive oil 5.0
Tocopherol acetate 0.2
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2,000) 13.0
Ethanol 7.0
Ethylparaben 0.2
Fragrance Appropriate amount of ion-exchanged water Residual Example 12 Solid foundation (prescription) (%)
Talc 43.1
Kaolin 15.0
Sericite 10.0
Zinc flower 7.0
Titanium dioxide 3.8
Yellow iron oxide 2.9
Red iron oxide 1.0
Black iron oxide 0.2
Squalane 8.0
Isostearic acid 4.0
Monooleic acid POE sorbitan 3.0
Isocetyl octoate 2.0
Calcium pantothenate 0.5
Preservative Appropriate amount Fragrance Appropriate amount Example 13 Emulsified foundation (cream type)
(Prescription) (%)
Titanium dioxide 10.3
Sericite 5.4
Kaolin 3.0
Yellow iron oxide 0.8
Bengala 0.3
Black iron oxide 0.2
Decamethylcyclopentasiloxane 11.5
Liquid paraffin 4.5
Polyoxyethylene-modified dimethylpolysiloxane 4.0
Ion exchange water 50.0
1,3-butylene glycol 4.5
Pantothenyl ethyl ether 1.5
Sorbitan sesquioleate 3.0
Antiseptic agent

Claims (5)

  A laminin 5 production promoter comprising as an active ingredient at least one compound selected from the group consisting of pantothenic acid and salts thereof, and pantothenyl ethyl ether.   The laminin-5 production promoter according to claim 1, which is exclusively for preventing or treating skin disorders associated with structural changes in the basement membrane.   The laminin 5 production promoter according to claim 1, which is exclusively used for healing wounds.   The laminin-5 production promoter according to claim 1, which is exclusively for regulating epidermal differentiation. The ability to produce laminin-5 in epidermal keratinocytes of the skin by externally applying to the human skin as an active ingredient at least one compound selected from the group consisting of pantothenic acid and salts thereof, and pantothenyl ethyl ether How to improve.