JP2007016025A - Topical composition for skin and method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a topically used composition used for recuperation and moisture retaining of skin, removal of acne, acne vulgaris and comedones äopen comedones (blackheads), closed comedones (whiteheads), papules or pustules} and anti-oxidation without containing vitamin A acid readily causing adverse effects on the skin. <P>SOLUTION: The topically used composition for skin is composed of principal ingredients of vitamin C in an amount accounting for 1-45 wt.%, vitamin B group in an amount accounting for 1-5 wt.%, carotene in an amount accounting for 0.1-3 wt.%, vitamin E in an amount accounting for 2-90 wt.%, a perfume in an amount accounting for 0.1-2 wt.%, a thickener in an amount accounting for 1-5 wt.%, a surfactant in an amount accounting for 1-8 wt.% and the balance of an adequate amount of distilled water. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a topical composition for topical use of the skin, and more particularly to a composition containing no vitamin A acid used locally for skin nourishing, moisturizing, acne, acne, youthful wrinkles and antioxidants.

  The structure of human skin encompasses the epidermis, dermis, subcutaneous adipose tissue, sebaceous glands, sweat glands, hair and nails, of which the dermis is a single layer of epidermal thickness, and the hair is surrounded by the surrounding hair follicle Has sebaceous glands. When the sebaceous glands secrete sebum, the sebum penetrates the skin surface through the hair and hair follicles, and then becomes a fat thin film that adheres to the skin surface and protects the skin. The skin is classified according to the number of sebaceous glands and is usually classified into oily skin, dry skin and mixed skin.

  Acne is usually recognized as acne and youth buds, and belongs to a kind of chronic inflammation of the hair follicle and sebaceous glands. However, youth buds usually appear in places with hair follicles throughout the body, such as the forehead, four nose wings, both cheeks, back, front chest, thighs, etc.Acne type, redness type and Divided into cyst type. Men and women in adolescence, when the gonads begin to mature, the male hormone content in the testicles, ovaries, and suprarenal glands increases. It deposits on the surface and reddens by bacterial action. Adolescent pupae usually have blackheads or baldheads first, and then acne is further infected with bacteria to develop symptoms such as papules, pustules, nodules, and cysts. In addition, if too much male hormone is secreted, red, wrinkles, heat, burns, and molting may appear on the T-shaped part of the face and the skin on the subcutaneous surface of the eye, and sometimes a distinct capillary phenomenon appears. This is called seborrheic dermatitis.

  Usually, an external preparation is expected to generate skin cleansing and bactericidal action, prevent evaporation of skin moisture, and enhance moisturizing action. For example, Patent Document 1 describes a balsam that can soften and smooth the skin by containing a sodium chloride component, and Patent Document 2 can cleanse the skin hygienically by containing mineral salts. It is disclosed. Patent Document 3 describes sugars that can be mixed with sucrose to lubricate the skin, and Patent Document 4 describes shaving using a sucrose aqueous solution.

  Sodium chloride can be the main body that maintains the body fluid osmotic pressure. Especially, 0.9% solution is called physiological saline, but it cannot absorb high concentration sodium chloride solution from the skin. Cause dehydration. Therefore, the addition of sodium chloride can irritate the skin or bacteria, but it is extremely difficult to improve the effect of preventing moisture evaporation and promoting the moisturizing action.

  Sucrose improves the quality of sugar and enhances cell functions throughout the body to enhance the metabolic function of the organism, but it also has a disinfecting effect. But if not oral supplementation, supplementation is provided through intravenous or skin injection. Even if sucrose is added to an external preparation directly applied to the skin, it cannot be expected that these sucrose will have a specific effect.

  Various locally used pharmaceutically active agents are added with a percutaneous absorption enhancer, and usually dibutyl hexanedioate and a mixture of hexanedioic acid and isopropyl bean (Amomum costatum) are employed. It is known that isopropyl bean succinate is already a locally formulated penetration enhancer. However, the applicant has not found from any prior art the combined effect of promoting skin penetration of DBA and IPM compositions.

  Various therapeutic and cosmetic agents, such as hydrogenated cortisone for pruritus and red spots in anaplastic dermatitis, sulconazol nitrate for dermatophyte infection, tretinoin for photoaging, and 5-fluorine for psoriasis and skin cancer Uracil is used in the treatment of several skin conditions. For the treatment of skin diseases, penetration enhancers such as dimethyl sulfoxide (DMSO, dimethylformamide, methyl decyl sulfoxide (Patent Document 5), dimethylacetamide (Patent Document 6) and N-alkyl-2-camphenylone (Patent Document 7) are usually added. However, the penetration enhancers have a number of drawbacks, such as dimethyl sulfoxide, which has a nasty taste and body odor, causing burns and red spots in the skin, reducing the transparency of the lens skin and severely causing animal tissue necrosis. (Non-patent Document 1) Dimethylformamide and dimethylacetamide also cause burns and red spots in the skin.

  Trebosc et al. In US Pat. No. 6,037,099 published compositions of cosmetic compositions containing modified caffeine derivatives as active agents have “good and long-lasting lipolytic properties, so slimming plans and sebum particles It has been proven to be “very effective in therapy”. Mausner, Patent Document 9, uses methylsilanol theophyllinacetate alginate and methylsilanol mannuronate as components of anti-cellulite.

  For example, if the retylene of Kligman of Patent Document 10 is applied locally, fat grains can be finitely improved. Kligman says that moderate to sharp improvements can be observed from increased skin thickness, increased new blood vessels, and twist test (column 6, lines 50-61). Patent Document 11 discloses aminolevulinic acid-photodynamic therapy “Topical aminolevulinic acid-photodynamic therapy for the treatment of acne vulgaris”. Patent Document 12 discloses a composition for treating acne using water and glycol. Patent Document 13 discloses a composition for treating acne using reveratrol (3,4 ', 5-trihydroxy-trans-stil bene), melatonin, vitamins D, E, and A. Patent Document 14 uses biotin, biotin ester, hydroxycarboxylic acid, antioxidant, and Patent Document 15 discloses vitamin B group vitamins, vitamin E, vitamin C, phosphorus Disclosed using phospholipids to protect the skin.

From the above materials, a number of patents allege that they have therapeutic effects on acne treatment, fat removal, and treatment of several skin conditions, all of which are vitamin A acids of the present invention. It is different from the local use composition which does not contain. The main purpose of the present invention is to rejuvenate, moisturize and treat acne, acne, youthful wrinkles and antioxidants using a composition that does not contain vitamin A acid locally.
US Patent 3,574,854 German patent 3,327,840 US Patent 3,859,436 US Patent 3,777,597 US Pat. No. 3,527,864 U.S. Pat. No. 3,472,931 US Pat. No. 3,696,516 US Patent 5,030,451 US Pat. No. 5,215,759 US Pat. No. 5,051,449 US Patent Application Publication No. 20020099094 US Patent Application Publication No. 20020061855 US Patent Application Publication No. 20010056071 US Pat. No. 5,710,177 US Pat. No. 4,938,960 (Mart idale, The Extra Pharmacopeia, pages 1461 to 1463, Twenty-Seventhedition, 1977)

  The present invention discloses a topical skin-use composition that does not contain vitamin A acid, and is a composition used for the treatment of skin rejuvenation, moisturizing acne, acne, and youthful wrinkles. shapiross and saliouc were prepared by blending vitamin A, vitamin D and its derivatives with antioxidants such as vitamin C, vitamin E and coenteam Q in 2001 Nutrition Vol. 17, 10th pp. 839-444. Explained that it can improve and treat acne. However, it does not provide an overall practicable formulation. This is because the above vitamins are important substances that cause the physiological activity of the human body.

  Vitamin A acid is also called Tretinoin, Retnoids, and belongs to a kind of derivative of vitamin A structurally. The main effect of A acid is to remove keratin chemicals. The A acid removes the uppermost stratum corneum of the stratum corneum of the epidermis, so that it can improve the blockage of hair pores, skin wrinkles, Improves circulating blood flow, reduces pigment scars and disrupts skin keratinization, promotes replacement of epithelial cells, promotes shedding, suppresses synthesis of keratin, and reduces formation of facets Can be prevented. However, many vitamin A acid products cause photosensitivity to the skin, and when used excessively, there are side effects that cause dry skin, peeling, fever, itchiness, dermatitis and the like.

  Vitamin B group vitamins are not a single type, but a combination of dozens of vitamins, including vitamin B1 (thiamin), vitamin B2 (riboflavin), niacin, vitamin B5 (pantothenic acid), vitamin B6 (pyridoxal), It contains folic acid (tolic acid), vitamin B12 (cobalamin), and biotin. Its main function is that of coenteam, responsible for sucrose oxidation and fat and protein energy release. It maintains the normal function of the nervous system and is essential for growth, cell regeneration, and synthesis of nuclear proteins and myelin. It can promote the production of red blood cells by activating folic acid and assisting the enzyme.

  Vitamin E is also recognized as an antioxidant, which suppresses platelet aggregation, prevents oxidation of erythrocyte cell membranes and prevents it from being destroyed and causing anemia. Maintains cell membrane integrity and promotes normal function of flax oil. By protecting the structure and function of the skin and nerve tissue and increasing the blood flow of the terminal blood vessel, the situation can be effectively improved. Vitamin A has the above-mentioned activity, but among recent commercial brands, for example, a product that combines vitamin E with Wal Green contains vitamin E, Loreal Furtur E or Jasom Natural Cosmetics, It is not described as having the function of treating acne, acne, youthful candy and antioxidant. In addition, examples prepared from the main component of the composition of the present invention from the activity test of the “topical use composition” of the present invention are shown to have a good effect. Therefore, it should be emphasized that the “locally used composition” of the present invention is composed of vitamin C, vitamin B group, carotene, vitamin E and a fragrance, a thickener, and a surfactant. It cannot be suggested or estimated from.

  The “topical use composition” of the present invention is composed mainly of vitamin C, vitamin B group, carotene, vitamin E, flavor, thickener, and surface activity. Since some of the components in the composition are vitamins essential to the human body and the main component does not contain vitamin A acid, there is no doubt of overdose or bone relaxation even after long-term use. Vitamins are in low demand in the body, but despite their vital function, the human body cannot be self-synthesized and must be offered to the outside world. Vitamin C prevents the formation of lipid peroxide in the human body, promotes the formation of collagen, relaxes the function of cell aging as a coenteam of many enzymes, and can gradually reduce black pigment. Therefore, it is recognized that it contributes to the regeneration of skin, and it can suppress the generation of black pigment and enhance immunity.

  US Pat. No. 5,834,445 describes that mucosal langerhans cells enhance local immunity by including carotene and vitamins. However, technical problems exist in some balsams, emulsions, facial cream skins or cosmetic agents because carotene contains a rich color tone and belongs to fat solubility. The weight% of the main components of the “locally used composition” of the present invention is 1 to 45% for vitamin C, 1 to 5% for vitamin B group, 0.1 to 5% for carotene, 2 to 90% for vitamin E, and flavoring 0.1 to 2%, thickener 1 to 5%, surfactant 1 to 8%, and the remaining appropriate amount of distilled water. The preferred proportion of the main components is 4% by weight of vitamin C, 1 to 3% of vitamin B group, 0.1 to 3% of carotene, and 20 to 65% of vitamin E.

  The present invention relates to a composition that does not contain vitamin A acid. With the above composition, the skin rejuvenation, moisturizing and acne, acne, and youthful moxibustion are effective. Since the composition of the present invention is a complete formulation composed of vitamin C, vitamin B group, carotene, vitamin E, fragrance, thickener and surfactant, the activity exhibited by conventional oral single vitamin compounds Is different. In addition, since the present invention is applied directly to the affected area of acne, acne and youthful buds, it has the effect of antioxidant and removal of acne, acne and youthful folds, and is used in the local position of skin, face and other limbs. Achieves skin rejuvenation and moisturizing effects.

  In the present invention, various excipients, carriers or diluents are added if necessary, and cartilage, emulsion, detergent or patch is directly applied to the affected area. These coatings are made by adding a viscosity agent such as starch or sodium methylcellulose carboxylate according to the conventional formulation method, or adjusting the acid alkalinity with a buffer solution such as phosphate to reach an appropriate pH value. ing. These coating agents can be selected from natural plant extracts such as licorice by adding a penetration enhancer according to a conventional formulation method.

  The present invention has the therapeutic effects on skin rejuvenation, moisturizing and acne, acne and youth buds by the above composition. The composition of the present invention is a formulation composed of vitamin C, vitamin B group, carotene, vitamin E, fragrance, thickener and surfactant, and is different from the activity exhibited by conventional oral single vitamin compounds. is doing. In addition, since the present invention is applied directly to the affected area of acne, acne and youthful buds, it has the effect of antioxidant and removal of acne, acne and youthful folds, and is used in the local position of skin, face and other limbs. Achieves skin rejuvenation and moisturizing effects.

  After describing various embodiments of the overall topical use composition, and the objects and effects achieved, the details of the present invention will be further explained based on the maintenance method and activity experiment of the topical use composition.

Formula LO-107

  The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-108

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-109

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-110

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-122

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-130

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formulation LO-18

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formulation LO-27

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formulation LO-22

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

上記原料ビタミンＣ、ビタミンＢ-complexを少量の蒸留水と相互溶解する。他にビタミンＢ-カロチン、ビタミンＥ、香料、界面活性剤、増稠剤を混合する。しかる後、両溶液を混合し、蒸留水量を補足する。 Formula LO-39

The above raw material vitamin C and vitamin B-complex are mutually dissolved with a small amount of distilled water. In addition, vitamin B-carotene, vitamin E, fragrance, surfactant and thickener are mixed. Thereafter, both solutions are mixed to supplement the amount of distilled water.

Activity test 1: 60 patients (30 males and 30 females) with acne disease in a half-year outpatient from February to August 2001 using Lo-108 treatment effect assessment for acne disease patients , Age 17-42 years, average 25 years). Lo-108 was used to treat the local surface treatment for a total of 6 months, smeared daily for at least 3 hours, and a maximum of 8 hours, then removed. A visit was made once every two weeks and changes in acne quantity and properties were recorded. The use of details is as follows.

1. The affected area is first washed with a wash cream (soap), and then the skin is dried and then applied.
2. The amount of coating is 2ml left and right, and it is covered with gauze so that it does not overflow all four rounds.
3. Apply medicine (ointment) for at least 3 hours before washing. In this case, do not wipe off any moisturizing emulsion.
4). If there are pustules, crush them first before applying the medicine. If the disease has pustules, add oral antibiotics: treat with tetracyclomordine or Vibramycin for a week, and for the rest of the time, various acne treatments such as oral or topical vitamin A acid or hormonal treatment The use of was prohibited.

  The results were evaluated by examining the number of acne, papules, pustules, and swelling from the doctor and the patient, and determined the severity and percentage of improvement.

Results As shown in Table 1, the number of blackhead and whitehead acne clearly decreased from an average of 43.5 to 20.1 (P <0.01) after 8 weeks of treatment. And the opening reduction of the hair pore was seen, and the redness papule showed a more remarkable quantity reduction. The average descent from 21.0 to 2.1 showed P <0.001. The disappearance of the erythematous phenomenon of pustules and cysts dropped from an average of 8.9 and 0.8 to less than half, with only a few red spots and concave scars remaining. In fact, erythoma is already clearly improving at 4 weeks before use, and the desire to continually treat their acne using Lo-108 is shown. It was. During the evaluation, Lo-108 has the ability of extinguishing, lowering the temperature, and erythema, and the phenomenon of excessive keratinization and growth of the skin also decreased, and the skin was changed relatively smoothly. The improvement of the concave scars was somewhat improved only by those with a slight tumor. This is a phenomenon obtained after the disappearance of the tumor.

  The exfoliated topical or antibiotic topical drugs used in the past have always caused pains caused by dry molting of the skin, severe perceptions such as swelling, but Lo-108 has a moisturizing effect. Therefore, such a situation has not occurred. However, some test takers have put up with their benefits, admitting that their complexion and taste are relatively difficult to perceive.

  By combining the above data, it can be recognized that Lo-108 has a therapeutic effect for acne. The action needs to go one step further and explore each type of test. This is achieved, for example, by having an increase in the keratin water content effect as well as the potential for keratinization, a decrease in sebum secretion, the possibility of bactericidal anti-inflammation.

Test 2: Control evaluation of ability to suppress sebum overflow
Twenty healthy volunteers participated in the clinical trial in this section. The skin of the forehead of those who are between the ages of 18 and 25 is divided into left and right sides, one of which is smeared with Lo-108 and the other is not smeared. . As a smearing method, 1 ml to 2 ml of Lo-108 was smeared every night at 3 o'clock and then washed. This method was used for smearing for a total of 4 weeks. A round visit was made every week, and the amount of sebum in each of the left, right, and forehead was examined and measured. Each time, each received a test measurement within the same fixed time interval.

In the sebum overflow test, sebometer 810PC (Courage and Khazaka Ltd, Germany) was used to measure the amount of oil on the surface of the skin. . The principle of sebum surveying is to press 0.1mm thick, 64mm ² area opaque plastic material at the tip of the instrument on the skin surface for 30 seconds, and then absorb the sebum to increase the transparency, and linearly with the amount of sebum By increasing the amount of sebum, the higher the transparency, the higher the transparency. The photometer survey can be converted to milligram / cm ² through the formula.

Results Since the test group and the control group are the forehead of the same person, the measurement of oil on the left and right sides removed the interfering factors such as temperature in the climate, temperature change, degree of exercise of the examinee, and degree of sweating. The data was obtained using the statistical method of ANOVA (Analysis of variance), and it was shown that the forehead oil in the test group was clearly lower than the control group. As shown in Fig. 1, the average difference between weeks tends to become clearer. However, as shown in Table 2, according to the ANOVA statistical data table of Lo-108 sebum amount, the increase or decrease in the amount of sebum is between one type. There was already a clear statistical difference in use (Pr> F0.001). However, if calculated in terms of time, as shown in Table 3, the difference in the amount of fat and oil generated according to the length of time shows a similar result as the time of use increases, and increases with the passage of time. Therefore, there is an alternating relationship (Pr> F0.2854) without objection. From the above results, it was shown that Lo-108 can effectively remove or suppress oil secretion for more than 12 hours and relax the amount of open sebum (one of the main causes of acne) of the sebaceous glands for a while. Such an effect can be achieved within a short period of time, and it cannot be proved that the more it is used, the greater the effect is. However, if used daily, the inhibitory effect of sebum could be maintained for 4 weeks or more.

Test 3: Animal experiment of burn and scar restoration The male rat (wistar species) 8 weeks in size used in this experiment is a successful university animal that is the only animal breeding unit that meets the overseas requirements (SPF) of the weekdays. It is kept in the center. Rats are housed in an animal room with air conditioning and room temperature maintained at 25 ± 1 ° C. so that any rat can freely eat and drink.

Burn Experiment The steps of this experiment were mainly made with reference to the experimental method of kistle et al., Using the animals themselves and observed in comparison with controls. In the experiment, (a) each rat was first anesthetized with 65 mg / kg pentabarbital and comatose, then the back was divided into 4 fixed areas (each area was 4 cm ² ); (b) lashes in each area with a razor (C) After that, a high heat iron plate (temperature of about 80 to 85 ° C. left and right) was placed in four areas on the back for about 10 seconds to take a step of causing burns on the back of the rat. After these steps were united, the iron pieces were removed and the wounds were cleaned by simple disinfection with oxidol (37%). After that, the treatment is different according to the division of the area, and the control area does not receive the drug, and the other three areas receive vitamin E, the product base and the test product based on the wound area, respectively. The amount was smeared evenly on the wound. After application, the wound was protected by wrapping tightly with gauze immediately so that the wound was not infected with bacteria. The drug was changed once a day every day to observe changes in the wounds of the rats, and photographs were taken every day for evidence. After 7 consecutive days, the animals were killed and 4 sections of tissue were taken and pathological sections were made. He was commissioned by Dr. Shin Cheng Li, a pathologist specializing in Shinko Medicine, to evaluate changes in wound pathology.

Result From the burn recovery situation, the change in inflammation is relatively reduced at the site of product treatment. The results showed that there was no significant difference between the inflammation at the control site and the product base-treated site. However, the site of product treatment was relatively unchanged in inflammation.

Wound Restoration Experiment This experiment also uses male rats (wistar species) that are 8 weeks in size. According to the above experimental steps, the anesthetized rat's back is divided into 4 fixed areas (each area is approximately 4 cm ² ) with a razor. I shaved off the eyelashes in each area. After that, an incision was made about 1 cm long so that scars of the flesh layer could be seen in each area of the back with a scalpel. Subsequently, the wound was cleaned by disinfection with Okididol (37%). In the same manner, the treatments are different depending on the division of the area, and the control area does not receive the drug. In the other three areas, vitamin E, the product base and the test product are respectively administered based on the wound area. The amount was smeared evenly on the wound. After application, the wound was protected by wrapping tightly with gauze immediately so that the wound was not infected with bacteria. Each day, the drug was changed once a day to observe the recovery of the rat's scars, and photographs were taken every day for evidence. Finally, we compared each other based on the number of days required from restoration to normality, and showed that the smaller the number of days, the faster the restoration.

Results In terms of the recovery status of the cut, the number of days for restoration is short at the site of product processing, only about 7.13 ± 1.27 days (N = 8). However, the restoration of the scar on the target area took 11.00 ± 2.24 days (N = 8), and there was little difference from 10.13 ± 1.62 days (N = 8) of the product base treatment site (P> 0.05).

Test 4: Bacterial inhibition experiment This experiment was applied using three strains of Staphylococus aureus, Methicillin Restant (ATCC 33591), Staphylococcus aureus and Acne (Propionibacterium acnes (ATCC6919)). And then analyzed with 0.03 ul / ml, 0.1 ul / ml, 0.3 ul / ml, 1 ul / ml, 3 ul / ml, 10 ul / ml, 30 ul / ml, 100 ul / ml Lo-110 However, the bacterial inhibitory concentration was 100 ul / ml.

Test 5: Skin moisturizing effect Among 20 to 40 years old, 12 healthy men and women who had no drug hypersensitivity reaction to the skin in the past, no skin disease within 3 months, and no scar on both cheeks, combined 24 people were screened. Candidates must not smear any substance on the test site before or during the test period, reduce sweating and sunburn on both cheeks, sign the test consent form, and proceed with the following experiment. did.
The first group of 12 men and women smeared the Lo-127 prescription, and the second group of 12 men and women smeared the Lo-108 prescription. In each tester's left and right cheeks, 1 ml of the preparation was uniformly smeared on the left half (about 15 cm ² ) of the experimental group, and not smeared on the right half of the control group.
Candidates arrived at the test site at 4 pm, washed their face with a neutral wash, and after 15 minutes weighed the skin moisture baseline and smeared the product. After 30 minutes of smearing time, the preparation on the skin surface was lightly applied with wet paper and washed with a neutral washing solution. After 15 minutes, the skin water content (data) was measured. The moisture content of the skin was measured with Corneometer CM 825 (Courage and Khazaka Ltd. Germany). The skin water content was analyzed by ANOVA (Analysis of yariance).

Results As a result, Lo-107, Lo-108 two types of prescriptions were applied to the forehead area of the face. I discovered that.

註：20名病人の左顔頬にLo-108を塗抹するのを処理組とし、右顔頬に基剤をすりつけるのを対照組とする。

Note: The treatment group is smearing Lo-108 on the left face cheek of 20 patients, and the control group is rubbing the base on the right face cheek.

It is a figure which shows the skin moisturizing effect which used Lo-107 prescription for the frontal part of the face. It is a figure which shows the skin moisturizing effect which used Lo-107 prescription for the cheek part. It is a figure which shows the skin moisturizing effect which used Lo-108 prescription for the forehead part of the face. It is a figure which shows the skin moisturizing effect which used Lo-108 prescription for the cheek part.

Claims (6)

  Vitamin C, whose main ingredient is 1 to 45% by weight, vitamin B group, 1-5%, carotene, 0.1-3%, vitamin E, 2-90%, and fragrance, 0.1-2% And a 1 to 5% thickener, a 1 to 8% surfactant and the remaining distilled water.   The topical use according to claim 1, wherein the preferred proportion of the main ingredients is 4 to 15% by weight, vitamin C is 1 to 3%, carotene is 0.1 to 2%, vitamin E is 15 to 65% Composition.   Vitamin C, which has a therapeutic effect on acne, acne, and youth moxibustion, and whose main component is 1 to 45% by weight, vitamin B group that accounts for 1 to 5%, and carotene that accounts for 0.1 to 3%, 2 A topical composition composed of -90% vitamin E, 0.1-2% fragrance, 1-5% thickener, 1-8% surfactant and the remaining distilled water.   Vitamin C, which has skin rejuvenation and whose main component is 1 to 45% by weight, vitamin B group that accounts for 1 to 5%, carotene that accounts for 0.1 to 3%, and vitamins that account for 2 to 90% A locally used composition comprising E, a perfume occupying 0.1-2%, a thickener occupying 1-5%, a surfactant occupying 1-8% and the remaining distilled water.   The composition for local use according to claim 1, which is used at a local position of a human limb such as skin or face.   Vitamin C, which has an antioxidant effect and its main component accounts for 1 to 45% by weight, vitamin B group that accounts for 1 to 5%, carotene that accounts for 0.1 to 3%, and 2 to 90% A topical composition comprising vitamin E, a fragrance occupying 0.1-2%, a thickener occupying 1-5%, a surfactant occupying 1-8%, and the remaining distilled water.

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