JP2005104877A - Stress reliever - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a stress reliever that is derived from a natural product and is safe even by long-term internal use by searching a plant extract expected to make a person recover from stress influence and to ameliorate a depressed state and a food, a functional food, a beverage and a medicine containing the stress reliever. <P>SOLUTION: The stress reliever is obtained from black cohosh, Cimicifuga racemosa by scientific name, which is a plant belonging to the family Ranunculaceae. The stress reliever comprises actein or a plant extract containing actein as an active ingredient. The food, the functional food, the beverage and the medicine contain the stress reliever. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a stress relieving agent containing actin or a plant extract containing it as an active ingredient.

  Today is an era of stress overload, and more or less living in the present age lives under the influence of stress. The influence of such stress on the human body has recently been investigated in detail, and it is becoming clear that it has an unexpectedly large effect. In recent years, the number of criminal cases that have had a social impact is said to be due to the effects of such stress. Reducing the cause of stress is a fundamental solution, but it is difficult to do so at present, and food materials that reduce the effects of stress are desired.

Known are plant extracts and herbal medicine extracts that are expected to recover from the effects of stress and to improve depression due to stress. For example, leguminous confectionery, orchidaceae spruce, liliaceae aloe vera, coriaceae coral, coriaceae coral, mistletoe coriander, cicada cinamao, ginger ginger, legume kudu, buttercup genus, cirrus Foods that have an antidepressant effect, including an accelerator for recovery from adverse effects of stress (for example, see Patent Document 1) consisting of the essence of a herbal essence of Chinese herbal medicines, and a rafu extract (See, for example, Patent Document 2).

Black cohosh is a plant belonging to the family Ranunculaceae under the scientific name of Cimicifuga racemosa. Its main component, Cimicifugin, is known to relieve symptoms of menopause by suppressing rapid estrogen loss. As for the use of black cohosh extract, as an estrogen-type period selective drug for the treatment and / or prevention of cardiovascular diseases, especially atherosclerosis, osteoporosis and menopause, for example prevention or alleviation of transient heat sensation (Patent Document 3). However, no stress mitigating action is mentioned.

JP 2001-192338 A JP 2002-201139 A Japanese translation of PCT publication No. 2002-506827

  An object of the present invention is to provide a stress relieving agent that is naturally derived and that is safe even after long-term use.

  The present inventors have found that black cohosh has a stress relieving action (Japanese Patent Application No. 2000-370604). As a result of further intensive studies, it was found that Actein in black cohosh roots exhibits a particularly stress relieving action, and the present invention has been completed.

  Accordingly, the present invention is to provide a stress relieving agent containing actin or a plant extract containing actin as an active ingredient. Furthermore, foods, functional foods, beverages and pharmaceuticals containing the stress relieving agent are provided.

  The actin of the present invention or a plant extract containing actin exhibits a stress relieving action and is useful as a component of a stress relieving agent. In addition, actins or plant extracts containing actins can be used in compositions such as foods, nutritional supplements, functional foods, and pharmaceuticals intended to alleviate stress.

As the stress relieving agent in the present invention, actin itself or a plant extract containing actin can be used. The plant extract containing actin includes, but is not limited to, black cohosh extract. The black cohosh extract is an extract obtained by extracting and concentrating black cohosh with water, ethanol, hydrous ethanol, or an organic solvent, and obtained by purifying the extract obtained here using an ultrafiltration membrane. is there. Black cohosh leaves, stems, flowers, rhizomes and the like can be used for extraction, but rhizomes are preferred. The hydrous ethanol has an ethanol concentration of 10 to 95%, preferably 30 to 70%, and more preferably 50%.

  The present invention further provides a composition having stress relieving action comprising actin or a plant extract containing actin, which composition is preferably in the form of a food, a dietary supplement, a functional food or a pharmaceutical. The administration method when the composition of the present invention is a pharmaceutical composition is not particularly limited, but an orally administrable dosage form is preferred. The pharmaceutical composition of the present invention can be in various dosage forms. For example, for oral administration, tablets, capsules, powders, granules, pills, solutions, emulsions, troches, suspensions, solutions, spirits, syrups, extracts, elixirs Can be, but is not limited to. In addition, various pharmaceutically acceptable carriers can be added to the preparation. For example, excipients, binders, disintegrants, lubricants, flavoring agents, coloring agents, sweeteners, flavoring agents, solubilizers, suspending agents, emulsifiers, coating agents, vitamin C, antioxidants It can include but is not limited to these.

  The dosage of the pharmaceutical composition of the present invention is generally 0.1 mg to 200 mg, preferably 0.1 mg to 100 mg, as an adult daily dose of actin, and as a daily dose for an adult in terms of black cohosh extract. 1 mg to 2000 mg, preferably 1 mg to 1000 mg is used. Of course, it can be changed individually according to the age, weight, symptoms, administration route, administration period, course of treatment, etc. of the administered human. The daily dose can be administered in several divided doses. It can also be administered in combination with other stress relieving agents and treatments.

  The composition of the present invention may be in the form of a food or a dietary supplement. For example, it can be made into the form of noodles, bread, candy, jelly, cookies, soup, and health drinks by blending actin or a plant extract containing actin into the raw material. Such foods, dietary supplements and functional foods include actins or plant extracts containing actins, inorganic components such as iron and calcium, various vitamins, dietary fibers such as oligosaccharides and chitosan, soybeans Proteins such as extracts, lipids such as lecithin, saccharides such as sucrose and lactose can be added.

In addition, the actin or actin-containing plant extract of the present invention is a rabu extract, a ginseng leaf extract, a ginkgo biloba extract, a camellia extract, an elephant extract, a peony extract, a guarana extract, a maca extract, Dandelion extract, artichoke extract, gentian extract, shisandra extract, western pumpkin extract, soy extract, licorice extract, toki extract, western carrot extract, pomegranate extract, yam extract, giant grasshopper extract Foods, nutritional supplements, functional foods, pharmaceuticals, etc. that have the effect of alleviating symptoms caused by mental stress by combining foods, yellow-tailed aloe extract, rice carrot extract, chabodiai extract, mistletoe extract, etc. A composition is obtained.

Study on Actein extraction, purification and isolation
Black cohosh roots and rhizomes (5.2 kg) were extracted with hot methanol (3 hours, twice), and the extract was concentrated under reduced pressure. Suspend 445 g of concentrated extract in 30% methanol, apply to DiaionHP-20 column chromatography, elute with 30% methanol, 50% methanol, methanol, ethanol, ethyl acetate in order of decreasing polarity, It was fractionated into minutes. 181 g of the obtained methanol-eluted fraction was separated into 7 fractions (fractions A to G) by silica gel column chromatography [chloroform-methanol (19: 1; 9: 1; 4: 1; 2: 1) → methanol]. I drew it. Fraction C was further purified by silica gel column chromatography [chloroform-methanol (19: 1)], octadecylsilanization (ODS) silica gel column chromatography [acetonitrile-water (1: 1)], Sephadex LH-20 [methanol], and further 5 Fractions were fractionated (fractions C-1 to C-5). Fraction C-3 was dissolved in methanol and allowed to stand at room temperature, resulting in precipitation. The precipitate was collected by filtration, silica gel column chromatography [chloroform-methanol (30: 1; 19: 1)], ODS
Purification by repeated silica gel column chromatography [acetonitrile-water (1: 1)] and semi-preparative ODS HPLC [acetonitrile-methanol-water (1: 1: 1)] yields actin (3.2 g). Released.

Examination of stress relieving effect 6 Examination was performed using BALB / c male mice of appropriate ages. Actin 100 mg / kg was orally administered at the age of 7 weeks after preliminary breeding for 1 week. From 30 minutes after the administration, a stress for trapping the mouse in a 30 ml syringe for injection was applied for 1 hour, and blood was collected immediately after that to measure the corticosterone concentration and LHD concentration in plasma. As shown in Table 1, the test group significantly suppressed the increase in each parameter as compared with the control group.
Table 1: Stress relieving effect of Actin

Corticosterone (ng / m1) LDH (IU / 1)

Control group 687.0 ± 32.0 98.4 ± 54.5
Actin group 361. 0 ± 41.3 3 ** 345.5 ± 44.5 5 **

(N = 5, mean ± standard deviation, ** p <0.01)
As shown in Table 1, the test group significantly suppressed the increase of each parameter in a dose-dependent manner as compared with the control group.

(Troche)
Gum arabic 6.0
Magnesium stearate 3.0
Glucose 73.0
Lactose 17.6
Dibasic potassium phosphate 0.2
Potassium phosphate 0.1
Perfume 0.0995
Actin 0.0005
Total 100.0
Using each component of each of the above parts by weight, a lozenge was prepared according to a conventional method.

(candy)
Sucrose 20.0
Chickenpox (75% solids) 70.0
Water 9.5
Coloring agent 0.45
Perfume 0.0495
Actin 0.0005
Total 100.0
Using each component of each of the above parts by weight, it was made into a candy according to a conventional method.

(Chewing gum)
Gum base 20.0
Calcium carbonate 2.0
Lactose 77.0
Stevioside 0.0995
Actin 0.0005
Fragrance 0.9
Total 100.0
A chewing gum was prepared in accordance with a conventional method using each component of each part by weight.

(juice)
Concentrated tangerine juice 15.0
Fructose 5.0
Citric acid 0.2
Fragrance 0.1
Dye 0.15
Sodium ascorbate 0.0498
Actin 0.0002
Water 79.5
Total 100.0
Using each component of the above-mentioned parts by weight, juice was prepared according to a conventional method.

(cookie)
Soft flour 32.0
Whole egg 16.0
Butter 16.0
Sugar 25.0
Water 10.8
Baking powder 0.1998
Actin 0.0002
Total 100.0
Using each component of the above-mentioned parts by weight, a cookie was prepared according to a conventional method.

(caramel)
Granulated sugar 31.0
Chickenpox (75% solids) 20.0
Milk powder 40.0
Hardened oil 5.0
Salt 0.6
Perfume 0.0295
Actin 0.0005
Water 3.37
Total 100.0
Caramel was prepared according to a conventional method using each of the above components by weight.

(Tablets and capsules)
Actin 1.0
Lactose 84.0
Magnesium stearate 15.0
Total 100.0
The above respective parts by weight were uniformly mixed, and tablets and capsules were prepared according to a conventional method.

(Powder, granule)
Actin 2.0
Starch 48.0
Lactose 50.0
Total 100.0
The above respective parts by weight were uniformly mixed to obtain powders and granules in a conventional manner.

(Injection)
Actin 0.1
Surfactant 9.0
Saline 90.9
Total 100.0
Each of the above weight parts was mixed by heating and sterilized to obtain an injection.

Claims (4)

A stress relieving agent containing actin as an active ingredient. A stress relieving agent comprising a plant extract containing actin as an active ingredient. A composition having a stress relieving action, comprising actin or a plant extract containing actin. The composition according to claim 3, wherein the composition is a food, a dietary supplement, a functional food or a pharmaceutical.