JP2005055352A - Diagnostic device and diagnostic system loaded with diagnostic device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problem in a conventional perioperative quick diagnosis wherein a time is required until diagnostic information is acquired and an operation is discontinued until acquisition, because steps are required beforehand wherein a living tissue resected partially by an operating doctor in an operating room is carried to a pathologic examination room, and the living tissue treated by an examination engineer is diagnosed by a pathological doctor, and finally the diagnostic information is reported to an operating doctor. <P>SOLUTION: In this diagnostic device and this diagnostic system loaded with the diagnostic device, histopathological information is detected from the living tissue of a patient during the operation by a gene information detection part provided on an operating instrument, and collated with a pathologic database provided on a separated place by communication between a detection treatment/information transmission part and an information reception part, and confirmation or judgement by the pathologic doctor is performed relative to acquired diagnostic information, and the diagnostic information is reported quickly to the operating doctor through communication. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a diagnostic apparatus that mounts a sensor capable of diagnosing a tissue / lesioned part on a surgical instrument and accurately performs diagnosis in a short time during surgery, and a diagnostic system that includes the diagnostic apparatus.

  In general, if there are unexpected findings on the surgeon's initial findings during surgery, or if the extent of resection must be determined on the spot, it may be desirable to obtain histopathological information immediately. is there.

  The diagnosis performed at this time is generally referred to as “intraoperative rapid pathological diagnosis” (hereinafter referred to as intraoperative rapid diagnosis). This intraoperative rapid diagnosis is an examination that makes a tissue sample during the operation and makes a diagnosis, and determines the effect that was removed during the operation or whether it should be removed further, and the therapeutic effect is improved. Is an important test that is expected.

  For example, Patent Literature 1 discloses a microscope remote observation system that uses a microscope remote observation system in this intraoperative rapid diagnosis. In this microscope remote observation system, the pathologist of the remote operator operates the observation side terminal, and remotely observes the microscope through the microscope terminal unit installed in the pathology room connected by the transmission path and separated. This is a system for giving advice from a pathologist to a doctor in charge in a pathology room.

As a diagnostic technique, Patent Document 2 discloses a medical system in which diagnosis is performed using genetic information of a patient.
JP-A-7-95556, JP 2002-117138 A

  Currently, an intraoperative rapid diagnosis is carried out by carrying a lesion part partially cut by a surgeon in an operating room to a pathology laboratory and processing the tissue by an examination engineer. The processed lesion or living tissue is passed to the pathologist and diagnosed using a microscope or the like.

  It takes at least a dozen minutes for the diagnosis result to return to the surgeon, during which time the operation is interrupted and the patient is in a waiting state with the surgical site open. Moreover, since it is necessary to make a diagnosis within a limited time based on the experience of a pathologist, there is a risk that the diagnostic accuracy may be lower than that of a normal pathological examination. Such a situation places a heavy burden on the surgeon and patient during the operation. Moreover, since a pathologist must make an accurate diagnosis within a short time, rich experience and a lot of knowledge are required.

  Therefore, the present invention is equipped with a sensor for detecting histopathological information in a part of the surgical instrument, and the surgeon contacts the tissue site where the surgeon wants to know the information during the operation, so that the diagnosis result ( It is an object of the present invention to provide a diagnostic apparatus that can obtain a genetic diagnosis result) and can realize a reduction in diagnosis time during surgery and an improvement in accuracy.

  In order to achieve the above object, the present invention obtains a part of a living tissue and performs hybridization to detect gene information of the living tissue, and the gene information detected by the gene information detecting means. Transmission / reception means for converting to electrical signals for communication, pathology database in which a plurality of diagnostic information associated with a plurality of types of gene information is stored in advance, and reference to the pathology database for the gene information received from the transmission / reception means Then, there is provided a diagnostic apparatus comprising diagnostic information search means for searching for relevant diagnostic information and display means for displaying the diagnostic information searched by the diagnostic information search means.

  And a forceps having a gene information detection mechanism that includes a DNA chip at the tip of the jaw, performs hybridization on a biological tissue attached to the DNA chip, converts optically detected gene information into an electrical signal, and outputs the electrical signal. , Connected to the handle side of the forceps, supplying and collecting the chemical solution for performing the hybridization, propagating the irradiation light to the DNA chip, converting the converted genetic information into a radio signal and transmitting it A detection processing / information transmitting unit, an information receiving unit located apart from the detection processing / information transmitting unit, receiving a radio signal from the detection processing / information transmitting unit, and decoding the genetic information, and a plurality of A pathological database in which diagnostic information associated with the genetic information of the species is pre-stored, and the received genetic information is referred to the pathological database, A diagnostic information retrieval unit that retrieves the diagnostic information, a confirmation display unit that is displayed to confirm whether the diagnostic information retrieved by the diagnostic information retrieval unit is correct, and a confirmation display unit that confirms that the diagnostic information is correct. And a display unit for displaying the diagnostic information in the vicinity of the location where the forceps are located.

  The diagnostic device configured as described above detects histopathological information during an operation in an operating room or the like, makes a reference to a database provided at another location by communication, and quickly obtains diagnostic information. Can be sought.

  In addition, the diagnostic system detects histopathological information during an operation in an operating room or the like, makes an inquiry with a database provided at another place by communication, and for the obtained diagnostic information, The pathologist is confirmed and diagnosed, and the diagnostic information can be quickly notified to the surgeon. Accordingly, the time required for diagnosis is shortened, that is, the time required for surgery can be shortened and the accuracy of diagnosis is improved, so that the burden on the surgeon and patient during the surgery is reduced.

  According to the present invention, a genetic information detection unit for detecting histopathological information is provided in a part of the surgical instrument, and the surgeon is brought into contact with the tissue site where the surgeon wants to know information during the operation. It is possible to provide a diagnostic apparatus that collects a part of a living tissue and obtains diagnostic information confirmed by a pathologist on the spot, thereby realizing a reduction in diagnosis time and an improvement in accuracy during surgery. The diagnosis system of the present invention uses highly accurate genetic information for diagnosis, and exchange of information and diagnosis information is performed by communication as in the past, so that the diagnosis information is returned to the surgeon in a short time. The operation interruption time is shortened.

Hereinafter, the best mode of the present invention will be described in detail with reference to the drawings.
FIG. 1A is a block configuration diagram showing an overall configuration example of the diagnostic apparatus of the present invention, and FIG. 1B is a block configuration diagram showing a configuration example of the gene information detection unit shown in FIG. It is.

  As shown in FIG. 1 (a), the diagnostic apparatus 1 includes a gene information detection unit 3 provided in a grasping unit 2 for grasping a biological tissue (or lesioned part) 100 desired to be examined, and gene information. Information reception for receiving a detection processing / information transmission unit 4 connected to the detection unit 3 by a cable (tube) and a spot information signal (such as gene information of the living tissue 100) transmitted from the detection processing / information transmission unit 4 Unit 5, an information processing unit 6 comprising a personal computer for processing received information, a display unit 7 for displaying processed information and operation instructions, an operation panel, a keyboard, etc. An input unit 8 for inputting instructions for the pathology, a pathological database 9 constructed by a database of various biological tissues, a database of DNA, and the like, and a pathology database 9 by an instruction from the information processing unit 6 Diagnostic information retrieval unit 10 that retrieves detected gene information for recorded information and obtains corresponding diagnostic information, and a display unit that incorporates a diagnostic result based on the diagnostic information obtained from diagnostic information retrieval unit 10 And a pathologist confirmation unit 11 composed of a personal computer or the like displayed on the screen and confirmed by a pathologist (for example, malignant or benign).

  In this diagnostic apparatus, for example, the grasping unit 2, the gene information detecting unit 3, the detection processing / information transmitting unit 4, the information receiving unit 5, the information processing unit 6 and the display unit 7 are arranged in an operating room where a surgeon is present. Is done. The pathology database 8, the diagnostic information search unit 9, and the pathologist confirmation unit 10 do not need to be arranged in the operating room, but are connected by a communication line 38 such as a line cable such as a LAN and arranged in a separate room such as a central management room. Also good. Instead of the communication line 38, a network such as the Internet may be used to communicate with an operating room in a remote hospital. Furthermore, the communication line 38 may use not only wired communication using a line cable but also wireless communication or optical communication. In particular, optical communication is suitable for a large amount of information such as an image.

  As shown in FIG. 1 (b), the gene information detection unit 3 includes an action part 12a provided on the lower grip part 2a of the grip part 2 and an action part 12b provided on the upper grip part 2b. When gripped, the action part 12a and the action part 12b face each other in a sealed state. Among these, the action unit 12b includes an optical cable 39a that propagates irradiation light, which will be described later, a cable 39b for an electrical signal (gene information) by photoelectric conversion of reflected light, and a cleaning solution and a target solution (fluorescence for hybridization and cleaning). A sample containing a substance and reacting with a specific DNA (hereinafter referred to as a sample) is connected to the detection processing / information transmitting unit 4 by a tube 39c or the like for feeding a chemical solution. Furthermore, the action part 12a is connected to the detection processing / information transmission part 4 by a discharge tube 40 for discharging the chemical solution. These cables and tubes are integrated together.

  2A shows a configuration of the action portion 12a viewed from above, and FIG. 2B is a diagram showing a cross-sectional configuration (AA) of the action portion 12a of FIG. 3A shows a configuration of the preprocessing / observing unit 21 of the action unit 12b as viewed from above, FIG. 3B shows an example of the configuration of the reference cell 22, and FIG. 3 is a diagram showing a cross-sectional configuration of a cell 22. FIG. FIG. 4 shows and describes a specific configuration of the gene information detection unit 3 and the detection processing / information transmission unit 4. A gripping part 2 (2a, 2b) shown in FIG. 1B shows a gripping state in which the living tissue 100 is sandwiched between the action part 12a and the action part 12b.

  As shown in FIG. 2A, the action part 12a includes a DNA chip 14 and a discharge port 16a of the discharge path 16. The discharge port 16a is not limited to the illustrated shape and arrangement.

  The DNA chip 14 is spotted so that a large number of spots (or probes) 15 in which a plurality of types of DNA are solid-phased on a plate substrate made of a slide glass or the like are arranged in a matrix or staggered, so-called microarray. It has become. Here, the spots 15 are arranged in a matrix.

  The discharge path 16 discharges a chemical solution applied to the DNA chip 14, such as a sample or a cleaning solution, when performing hybridization or washing on the DNA chip 14.

  On the other hand, the action unit 12b is provided with a preprocessing / observation unit 21 including a large number of reference cells 22 arranged so as to face each of the spots 15 when held. A transparent member, for example, a glass plate 22a, for preventing the chemical solution from entering is provided on the front surface of the reference cell 22. In this example, in the reference cell 22, an irradiation port 24 for irradiating illumination light propagated to the optical cable 39a is disposed in the center, and the liquid ejection unit 25 and the light receiving unit 26 for the chemical liquid are disposed in an oblique direction. ing. Of course, it is not limited to such an arrangement. The light receiving unit 26 includes a photodiode 23, receives reflected light including fluorescent color from the spot 15, and includes a signal including fluorescent intensity generated by photoelectric conversion, and spot information signal having spot identification information and position information. Is generated. The photodiode 23 may be a semiconductor image sensor such as a CCD.

  As shown in FIG. 4, the detection processing / information transmission unit 4 is composed of a water supply unit 31, a suction unit 32, a light source unit 33, a detection unit 34, and a communication unit 35. These units are provided from a personal computer or the like. It is controlled by the control unit 36. Among these, the water supply unit 31 supplies a sample or a liquid for cleaning to the DNA chip 14 to the liquid discharge unit 25 through the water supply path (tube 39c). The suction unit 32 collects the chemical solution supplied to the DNA chip 14 via the discharge path 16.

  The light source unit 33 is optically connected to each irradiation port 24 of the reference cell 22 via the optical fiber 27, propagates light from the light source unit 33, and irradiates each spot with light. The detection unit 34 includes a light receiving unit 26 in the reference cell 22 and generates a spot information signal from the reacted spot 15. The communication unit 35 converts the spot information signal into a radio signal or an optical signal suitable for communication, and transmits the signal to the information receiving unit 5 through the antenna 37.

  In the liquid discharge unit 25, a heater for heating the sample to about 50 ° C. to 95 ° C. may be incorporated in the action unit 12b. This heating may be used when a PCR reaction is used.

  With such a configuration, the reference cell 22 irradiates all the spots 15 with the light transmitted through the optical fiber 24 and receives the reflected light including the fluorescent color (fluorescence intensity) from the spot 15 reacted by the hybridization by the photodiode 23. Then, it is transmitted as a photoelectrically converted electrical signal to the detection processing / information transmitting unit 4 through a cable.

Next, the operation of the thus configured diagnostic apparatus will be described.
In order to obtain genetic information of the biological tissue 100 such as a patient, the biological tissue 100 is grasped by the grasping unit 2. When this is held, a part of the surface of the living tissue 100 is attached to the DNA chip 14. Thereafter, the sample is removed from the living tissue 100 and the sample 2 is allowed to flow from the liquid ejection part 25 of the reference cell 22 in a closed state in which the grasping part 2 is closed, that is, the action parts 12a and 12b are combined. Wash. Then, light is irradiated from the irradiation port 24 to all the spots 15 of the DNA chip 14. The reflected light including the fluorescent color from each reacted spot 15 is received by the photodiode 23, and a spot information signal (gene information) including the fluorescence intensity is generated by photoelectric conversion. The spot information signal is subjected to predetermined processing such as amplification and noise removal by the detection unit 34, converted into a radio signal by the detection processing / information transmission unit 4, and transmitted to the information reception unit 5 by radio. . The information receiving unit 5 decodes the received spot information signal and outputs it to the information processing unit 6 as gene information.

  The information processing unit 6 performs diagnosis based on the gene information according to a predetermined sequence (program). First, this genetic information is sent to the diagnostic information search 10, where it is compared with data recorded in the pathological database 9 (pre-recorded data or past accumulated data) and corresponding data (diagnostic information). ) Is read out. The diagnostic information search unit 10 transfers the read diagnostic information to the pathologist confirmation unit 11 and displays it. The pathologist checks whether the diagnostic information is correct. Of course, if there is no corresponding data in the database and diagnostic information cannot be obtained, a pathologist makes a diagnosis. The confirmed items are transferred together with the diagnostic information to the information processing unit 6 and displayed on the display unit 7.

  The surgical method (for example, excision range) is examined while viewing the diagnostic information and the confirmation items or diagnostic information of the pathologist.

  As described above, the diagnostic apparatus of the present invention detects histopathological information in an operating room or the like, and performs communication with a database provided at another location by communication to confirm and diagnose a pathologist. The diagnostic information is quickly notified to the surgeon. As a result, the time required for diagnosis can be shortened, that is, the time required for surgery can be shortened, and the accuracy of diagnosis can be improved, thereby reducing the burden on the surgeon and patient during surgery.

  Next, a diagnostic system using the above-described diagnostic apparatus will be described as a first embodiment. FIG. 5 is a diagram illustrating a configuration example in which the grasping unit 2 and the gene information detection unit 3 of the diagnostic apparatus are mounted on the inspection forceps 41. In addition, about the component part of this diagnostic system, the same referential mark is attached | subjected to the site | part equivalent to the component part shown in FIG. 1 thru | or FIG. 4 mentioned above, and the detailed description is abbreviate | omitted.

  This inspection forceps 41 is a forceps for grasping a living tissue (or lesion) 100 desired to be examined. The lower jaw 41a and the upper jaw 41b of the tip of the inspection forceps 41 are provided with the gene information detection unit 3. The gene information detection unit 3 is connected to the detection processing / information transmission unit 4 by a cable (and a tube) from the handle 41c side through forceps inner diameter paths 42a and 42b provided inside the inspection forceps 41.

  In addition to these, the information receiving unit 5, the information processing unit 6, the display unit 7, the input unit 8, the pathology database 9, the diagnostic information search unit 10, and the pathologist confirmation unit shown in FIG. 11 will be omitted here.

  In this inspection forceps 41, the living tissue 100 is sandwiched between the lower jaw 41a and the upper jaw 41b, the living tissue 100 is brought into contact with the spot 15, and a part thereof is adhered. Then, after separating the biological tissue 100, the test forceps 41 is again gripped and sealed, and then a sample is injected to perform hybridization.

  In this genetic information detection unit 3, the action part 12a is provided on the lower jaw 41a, and the action part 12b is provided on the upper jaw 41b. In these gripping states, the lower jaw 41a and the upper jaw 41b are in a sealed state. In addition, a detection processing / information transmission unit 4 connected by a cable, a tube, or the like is provided on the handle 41c side.

  FIG. 6A shows a configuration of the action portion 12a provided on the inspection forceps 41 as viewed from above, FIG. 6B shows a cross-sectional configuration of the lower jaw 41a in BB of FIG. FIG. 6C is a diagram illustrating a configuration example of the spot 15 of the DNA chip 14.

  Spots (or probes) 15 of the DNA chip 14 in the inspection forceps 41 are alternately arranged in a plurality of rows, and around the DNA chip 14 are discharge ports 16a for sucking and discharging a chemical solution such as a sample or a cleaning solution. Is provided. Further, inside the lower jaw 41a, a discharge passage 16 is formed along the longitudinal direction toward the handle 41c side, and guide grooves 13 are provided on both inner side surfaces. The movable portion 41b at the distal end portion of the lower jaw 41a is opened, and the DNA chip 14 is slid into the slide groove 13 so that it can be replaced.

  Further, as shown in FIG. 6B, a discharge path 16 (forceps inner diameter path 42a) is formed in the lower jaw 41b with the DNA chip 14 fitted in the groove 13 below. The discharge path 16 is connected to a plurality of liquid discharge ports 16 a provided in the lower jaw 41 a in the vicinity of the DNA chip 14. The discharge path 16 passes from the back surface side of the DNA chip 14 toward the handle 41c and is connected to the suction unit 32 described above. The liquid discharge port 16a and the discharge path 16 are collected by the suction unit 32 after the sample or cleaning liquid applied to the DNA chip 14 is drawn to the handle 41c side when the DNA chip 14 is hybridized or washed. .

  On the other hand, the upper jaw 41b is provided with a preprocessing / observation unit 21 composed of a number of reference cells 22 equivalent to those described above so as to face each of the spots 15. A specific description of the reference cell 22 is omitted here.

Next, diagnosis by the diagnostic system using the inspection forceps 41 will be described.
The details of the description of the diagnostic system here are in accordance with the operation of the diagnostic device described above, and the characteristic features of this system will be described. First, the diagnostic system is activated and started up to a state where the gene information detection unit 3 can detect gene information.

  A surgeon or the like in the operating room grasps the living tissue 100 for diagnosing a patient or the like with the lower jaw 41a and the upper jaw 41b of the inspection forceps 41, and attaches a part of the surface of the living tissue 100 to the DNA chip 14. . Thereafter, the sample is removed from the living tissue 100, and in a sealed state where the lower jaw 41a and the upper jaw 41b are combined, the sample is allowed to flow from the liquid discharge portion 25 of the reference cell 22 to perform hybridization, react, and then wash. Then, all the spots 15 of the DNA chip 14 are irradiated with illumination light, and a spot information signal (gene information) including fluorescence intensity by reflected light obtained from each reacted spot 15 is generated and input to the information processing unit 6. .

  In this information processing unit 6, gene information is sent to the diagnostic information search unit 10, where it is referred to data already recorded in the pathology database 9 and corresponding data (diagnosis information) is read out. The diagnostic information search unit 10 transfers the read diagnostic information to the pathologist confirmation unit 11 and displays it. The pathologist checks whether the displayed diagnostic information is correct. Of course, a pathologist may make a diagnosis. For example, in the case of a diagnostic process for cancer surgery, it is only necessary to distinguish between cancer cells and normal cells. Therefore, normal biological tissue of a patient is ingested and a normal DNA pattern is recorded before surgery. Diagnosis may be performed by comparing with a DNA pattern collected from a lesion during surgery.

  The confirmed items are displayed on the display unit 7 provided in the operating room. The surgeon determines an operation method (for example, a resection range) while viewing the diagnosis information and the confirmation items or diagnosis information of the pathologist.

  As described above, the intraoperative rapid diagnosis by the diagnosis system of the present embodiment detects histopathological information in the operating room, performs communication with a database provided at another location by communication, and performs pathology. Confirmation and diagnosis are performed, and the diagnosis information is promptly notified to the surgeon. As a result, the time required for diagnosis can be shortened, that is, the time required for surgery can be shortened, and the accuracy of diagnosis can be improved, thereby reducing the burden on the surgeon and patient during surgery.

Next, a second embodiment according to the diagnostic system of the present invention will be described.
In the first embodiment described above, the inspection forceps 41 is provided with one DNA chip 14 and diagnosis is performed for one DNA chip area at one location. However, in this embodiment, a plurality of DNA chips are further provided on the inspection forceps 41. The area to be diagnosed at once is expanded.

  As shown in FIG. 7, a plurality of, for example, five DNA chips 43 a to 43 e are provided on the lower jaw 41 a of the inspection forceps 41. When the inspection forceps 41 are held, the upper jaw 41b is provided with pre-processing / observing portions 44a to 44e similar to those described above so as to face the DNA chips 43a to 43e. The other constituent parts are the same as those in the first embodiment described above. However, since the number and the processing amount of cables and the like corresponding to the increase of the DNA chip are increased, it is configured to cope with them.

  As in the first embodiment described above, the diagnostic system configured in this manner performs hybridization and washing after attaching the living tissue of the lesion of the subject or patient to the test forceps 41, and then DNA. React the chip. By irradiating light to these DNA chips 43a to 43e, intraoperative rapid diagnosis based on gene information obtained from reflected light is performed.

  As described above, when the inspection target range exceeds the inspection range (DNA chip area) according to the first embodiment, the inspection must be repeatedly performed. For example, diagnostic information information of a wider examination range can be obtained by one examination.

  Further, as a modification of the first and second embodiments of the diagnostic system, the genetic information detection unit 3 is mounted on the inspection forceps 41, that is, the forceps used in the laparotomy, for example, a laparoscopic operation as shown in FIG. You may mount in the apparatus 51 similarly. The components other than the gene information detection unit 3 construct the system using the same components as in the first embodiment.

  In this surgical instrument 51, the action part 12a and the action part 12b are provided at the respective distal end parts 52a and 52b of the grip part 52.

  Next, as a third embodiment of the present invention, an energy treatment instrument equipped with a diagnostic system will be described.

  In the first and second embodiments described above, the genetic information detection unit 3 is provided in the inspection forceps. However, in this embodiment, the genetic information detection unit 3 is installed in an energy treatment instrument capable of treating living tissue. It is provided. In the diagnostic system of the present embodiment, the components other than the energy treatment device are the same as those in the first embodiment described above, and the same reference numerals are assigned and detailed description is omitted.

  As shown in FIG. 9, the gene information detection unit 3 is provided at the tips 62 a and 62 b of both gripping units 62 of the energy treatment device 61. The treatment portion 63 is provided on the handle side 62 c of the grip portion 62. The energy treatment device 61 is connected to the treatment device drive control device 65 by a cable (including a sample tube) 64. In addition, the treatment instrument drive control device 65 is provided with a foot switch 66 for turning on and off the drive power supplied to the treatment section 63.

  The treatment instrument drive control device 65 includes a power supply unit 67 for driving the treatment unit 63, an output adjustment unit 68 for performing output adjustment in the power supply unit 67, and the detection processing / information transmission unit 4. The As described above, the detection processing / information transmission unit 4 includes the water supply unit 31, the suction unit 32, the light source unit 33, the detection unit 34, and the communication unit 35.

  The energy treatment device 61 thus configured first obtains gene information using the gene information detection unit 63 by the same procedure as that of the first embodiment described above. The surgeon judges from the diagnosis information based on the genetic information at the site (operating room) and treats the living tissue by the treatment unit 63.

  According to the present embodiment, the area to be excised can be immediately excised after diagnosis information based on genetic information is issued. As described above, diagnosis is performed in the field, and immediate treatment is performed according to the diagnosis information, thereby not only reducing the burden on the surgeon and patient during the operation, but also improving the accuracy of the diagnosis information.

  As described above, by using the diagnosis system of the present invention, information such as genetic information with high accuracy is used, and information and diagnostic information are exchanged by communication as in the past. Therefore, it takes only a short time for the diagnostic information to return to the surgeon, and the operation interruption time is shortened.

  Since the diagnosis system of the present invention can make a highly accurate diagnosis for a patient using genetic information, not only a lesion in surgery but also, for example, the allergic constitution of an individual patient at the time of drug administration. Drugs that may cause allergies or the like can be selected according to the presence or absence and the type of the drug. By recording this information on an IC card or the like, danger can be avoided by simply checking this IC card when administering a new hospital or a new medicine.

  Also, when emergency surgery is performed for an emergency patient and there is no time to investigate allergies in advance, the patient's DNA is examined even during the operation to identify drugs that cause allergies, etc. Risk can be avoided.

FIG. 1A is a block diagram showing an example of the overall configuration of the diagnostic apparatus of the present invention, and FIG. 1B is a diagram showing an example of the configuration of a gene information detection unit. FIG. 2A shows a configuration of the action portion as viewed from above, and FIG. 2B is a diagram showing a cross-sectional configuration of the action portion 12a. 3A shows the configuration of the preprocessing / observation unit in the gene information detection unit viewed from above, FIG. 3B shows an example of the configuration of the reference cell, and FIG. 3C shows the reference cell. FIG. FIG. 4 shows and describes a specific configuration with the detection processing / information transmission unit 4. It is a figure for demonstrating the diagnostic system using the diagnostic apparatus of this invention as a 1st Example. 6A shows the configuration of the preprocessing / observation unit in the gene information detection unit of the first embodiment viewed from above, FIG. 6B shows the configuration example of the reference cell, and FIG. c) is a diagram showing a cross-sectional configuration of a reference cell. It is a figure for demonstrating the diagnostic system using the diagnostic apparatus of this invention as a 2nd Example. It is a modification of the diagnostic system which mounts the diagnostic apparatus of this invention in the surgical equipment under a laparoscope. It is a figure for demonstrating the diagnostic system using the diagnostic apparatus of this invention as a 3rd Example.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Diagnostic apparatus, 2 ... Examination forceps, 2 ... Grasp part, 2a ... Upper side grip part, 2b ... Lower side grip part, 3 ... Gene information detection part, 4 ... Detection process and information transmission part, 5 ... Information reception part, DESCRIPTION OF SYMBOLS 6 ... Information processing part, 7 ... Display part, 8 ... Input part, 9 ... Pathology database, 10 ... Diagnostic information search part, 11 ... Pathologist confirmation part, 12a, 12b ... Action part, 13 ... Groove, 13a ... Guide groove 14 ... DNA chip, 15 ... spot (or probe), 16 ... discharge path, 17 ... liquid discharge port, 22 ... reference cell, 23 ... photodiode, 24 ... irradiation port, 25 ... liquid ejection part, 26 ... light receiving 27: optical fiber, 31: water supply unit, 33: light source unit, 34: detection unit, 100: biological tissue (or lesion).

Claims (7)

Gene information detection means for obtaining a part of the living tissue and performing hybridization to detect genetic information of the living tissue;
Transmission / reception means for performing communication by converting the genetic information by the genetic information detection means into an electrical signal;
A pathological database in which a plurality of diagnostic information associated with a plurality of types of genetic information is accumulated in advance;
Diagnostic information search means for referring to the pathological database for the genetic information received from the transmission / reception means and searching for the corresponding diagnostic information;
Display means for displaying the diagnostic information retrieved by the diagnostic information retrieval means;
A diagnostic apparatus comprising: The genetic information detection means is composed of a first action part and a second action part that are in a sealed state when gripped,
The first action part is
A DNA chip for arranging a plurality of spots (probes) capable of reacting with the biological tissue attached by the hybridization;
A discharge part for discharging the chemical solution hung on the DNA chip for performing the hybridization,
The second working part is
A reference for generating genetic information as an electrical signal by facing each of the spots when held, applying the chemical solution to the DNA chip, irradiating the cleaned spot with illumination light, and receiving the reflected light The diagnostic apparatus according to claim 1, further comprising a cell. The reference cell is
A liquid discharger for discharging the sample to be the chemical solution and the cleaning solution for performing the hybridization and cleaning to the spot;
An irradiation port for irradiating the spot with light after the reaction by the hybridization;
A light receiving unit that receives reflected light of the light irradiated to the spot and generates an electrical signal including genetic information of the spot reacted from the reflected light;
The diagnostic apparatus according to claim 2, comprising: A forceps having a gene information detection mechanism comprising a DNA chip at the tip of the jaw, performing hybridization on a biological tissue attached to the DNA chip, converting optically detected gene information into an electrical signal, and outputting the electrical signal;
Connected to the handle side of the forceps, supply and recovery of the chemical solution for performing the hybridization, propagate the irradiation light to the DNA chip, convert the electrical genetic information into a radio signal and transmit it A detection processing / information transmission unit;
An information receiving unit that is located away from the detection processing / information transmitting unit, receives a radio signal from the detection processing / information transmitting unit, and decodes the gene information;
A pathological database in which diagnostic information associated with multiple types of genetic information is stored in advance;
A diagnostic information search unit that queries the pathological database for the received gene information and searches for the relevant diagnostic information,
Confirmation display means for displaying the diagnostic information retrieved by the diagnostic information retrieval unit for confirming correctness;
A display unit for displaying the diagnostic information confirmed to be correct by the confirmation display means in the vicinity of the location where the forceps are located;
A diagnostic system comprising: The forceps is composed of an upper jaw, a lower jaw, and a handle for opening and closing them.
A DNA chip mounted on the lower jaw, on which the attached biological tissue is subjected to hybridization and washing, and a plurality of spots for detecting genetic information of the biological tissue are arranged;
A discharge path formed in the lower jaw for discharging the sample and the washing solution applied to the DNA chip for performing the hybridization and washing;
A liquid ejection part formed on the upper jaw and ejecting the sample and the cleaning liquid to the spot;
An irradiation port for irradiating the spot with light after the reaction by the hybridization;
A light receiving unit that receives reflected light of the light irradiated to the spot and generates an electrical signal including gene information from the reacted spot;
The diagnostic system according to claim 4, further comprising: In the diagnostic system,
The diagnostic system according to claim 4, wherein the genetic information detection mechanism is provided in an energy treatment device. In the diagnostic system,
The diagnostic system according to claim 4, wherein the gene information detection mechanism is provided in a laparoscopic surgical instrument.

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