JP2005047890A - Communication disturbing agent and communication disturbing method for trigonotylus caelestialium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a communication disturbing agent and to provide a communication disturbing method using the sex pheromone of Trigonotylus caelestialium. <P>SOLUTION: The communication disturbing agent for Trigonotylus caelestialium contains a mixture of n-hexyl n-hexanoate, (E)-2-hexenyl n-hexanoate and n-octyl n-butyrate at weight ratios of 1,000:(400-500):(10-100). The communication disturbance is induced in Trigonotylus caelestialium by the use of the agent. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a communication disruption method using a sex pheromone of Trigonotylus caestialium, which is an important pest of rice, and if this communication disruption method is used, the sex pheromone communication between males and females is disturbed. This contributes to the control of pests, such as by suppressing the generation density.

  Traditionally, the red bearded mosquito turtle has been considered a problem only in Hokkaido as a pest that causes spotted rice. However, in recent years, the occurrence and damage of this species has increased rapidly from the Sea of Japan side of the Tohoku region, mainly in the Hokuriku region, and it is becoming a major pest that causes speckled rice in these regions.

  The wintering generation and the first generation of Akahigehosomidorikamisukame live on the shores of paddy fields and the surrounding grassland, but after the heading, the second generation adults invade the paddy field and damage the rice and repeat the generation. Therefore, in this type of control, it is important to control the density of the wintering generation and the first generation to prevent entry into the paddy field and to prevent proliferation in the paddy field.

  Weed management methods have been studied from various angles to prevent invasion of paddy fields, but no technology has been established with many uncertain factors regarding the timing and scale of weeding. After invading the paddy field, there is no control means other than pesticide spraying, which is a major obstacle to reduced pesticide cultivation. Agricultural sites and markets require the establishment of new control methods that can replace pesticides.

  The communication disruption method using a sex pheromone has been widely put to practical use in lepidopterous pests, and has been used for about 400,000 hectares of crops in 2002, which is useful for reducing pesticide cultivation. In stink bugs, one successful case of a communication disruption test using a synthetic pheromone in Campromma verbasci has been reported (Non-patent Document 1), but it has not been put into practical use at present.

  The sex pheromone communication system in pest species that have been successfully disrupted by communication until now (including lepidopterous and crustacea) includes sex pheromone only in both sexes (generally females). It is synthesized and released to attract the opposite gender (generally male) with no exceptions.

  In the type in which both sexes biosynthesize pheromones, alarm pheromones are known, but there have been no examples of successful communication disruption using such pheromones. This is probably because the individual to be disturbed owns the pheromone, so that the threshold rises and the disturbance is unlikely to occur.

  The sex pheromone of Akagegesomidorikasukameka was identified as a 1000: 400: 30 mixture of n-hexyl n-hexanoate), n-hexanoic acid (E) -2-hexenyl, n-octyl n-butyrate (non- Patent Document 2).

  As described above, the sex pheromone is generally released only by a female and attracts a male. However, these sex pheromone components are normally present in the male body surface of the red bearded winged turtle. The sex pheromone component is biosynthesized by both males and females, and is significantly different from lepidoptera and existing stink bug sex pheromones (Non-patent Document 2).

  The cause of the lack of attractiveness in males with sex pheromones is unknown in the red beetle butterfly turtle, but in species where males and females biosynthesize sex pheromone compounds, such as this species, communication disruption is similar to alarm pheromones. It was thought that the control effect by can not be expected.

H.L.McBrien, G.J.R.Judo and J.H.Borden (1996) Can.Entomol. 128: 1057-1064. Kakizaki M. and H. Sugie, (2001) J. Chem. Ecol. 27: 2447-2458.

  An object of the present invention is to provide a communication disruptor and a communication disruption method using a sex pheromone of the red bearded mosquito turtle.

  In order to solve the above-mentioned problems, the present inventors have intensively studied the communication disruption method of the sex pheromone of the red beetle moss turtle, and as a result, n-hexanoic acid n-hexyl, n-hexanoic acid (E) -2-hexenyl and It was found that the use of a mixture obtained by mixing n-octyl n-butyrate at a weight ratio of 1000: (400 to 500) :( 10 to 100) causes a communication disruption phenomenon in this species, thereby completing the present invention.

  With the use of the communication disruptor according to the present invention, it is possible to effectively inhibit sex pheromone communication of the red bearded mosquito turtle, and it is possible to suppress the growth of this species in rice fields that could only be controlled with insecticides in the past. Can contribute to the establishment of technical pest control technology.

The n-hexyl n-hexanoate, n-hexanoic acid (E) -2-hexenyl and n-octyl n-butyrate, which are sex pheromones used in the present invention, can be synthesized by known methods. For example, if an alcohol and a carboxylic acid chloride are reacted in the presence of a base such as triethylamine, all three compounds can be synthesized by the same method. Specifically, n-hexanoic acid n-hexyl is 1-hexanol and n-hexanoic acid chloride, and n-hexanoic acid (E) -2-hexenyl is (E) -2-hexenol and n-hexanoic acid chloride. N-octyl n-butyrate is 1-octanol and n-butanoic acid chloride. The purity is preferably 98% or more.
n-hexyl n-hexanoate, n-hexanoic acid (E) -2-hexenyl and n-octyl n-butyrate are mixed so that the weight ratio is 1000: (400 to 500): (10 to 100). The

  Further, in the communication disruptor according to the present invention, an appropriate amount of an antioxidant such as butylhydroxytoluene, butylhydroxyanisole, hydroquinone, vitamin E, or an ultraviolet absorber such as 2-hydroxy-4-octoxybenzophenone may be added to the above components. good. The appropriate amount is, for example, 1 to 5% by weight of the antioxidant and 1 to 5% by weight of the ultraviolet absorber with respect to the weight of the sex pheromone mixture.

In the communication disruption method of the present invention, in order to maintain the above-mentioned sex pheromone substance contained as an active ingredient over a long period of time, it is provided with a function of controlling the release amount of, for example, polyethylene, polypropylene, ethylene-vinyl acetate copolymer, etc. It may be used by being filled in a container such as a thin tube made of plastic, a bag made of laminate, an ampoule, or may be used by impregnating a rubber cap or the like. The preferable usage-amount of the said pheromone substance is 10-200 g / ha.
In particular, in the case of the sex pheromone of Akagegehosomidikasikame, it is possible to create a preparation with excellent sustained release by using a high-density polyethylene tube.

Hereinafter, specific embodiments of the present invention will be described with reference to Examples and Comparative Examples.
Examples 1-2 and Comparative Examples 1-2
n-hexyl n-hexanoate, n-hexanoic acid (E) -2-hexenyl and n-octyl n-butyrate were mixed at a weight ratio of 1000: 400: 30, and diluted to 10 mg / 100 μl with n-hexane. (Examples 1-2). To this diluted solution, 2% by weight of butylhydroxytoluene was added as an antioxidant. 100 μl was taken out from the diluted solution, impregnated with isoprene rubber, and evaporated to use the communication disruptor.
^Two sections of 5 m × 5 m (25 m ² ) were separated from an approximately 0.5 ha Italian ryegrass field by 10 m or more. At the four corners of the communication disruption zone (Examples 1 and 2), each of the communication disruptors described above is placed at a height of about 50 cm above the ground, and the center of the red-bellied fossil midge turtle with reference to Non-Patent Document 2 is placed at the center. A basin trap equipped with an attractant was installed. In addition, empty disturbing preparations containing no synthetic pheromone were placed at the four corners of the untreated section (Comparative Examples 1 and 2), and a basin-type trap was placed at the center as in the case of the communication disturbing agent.
Every day after installation, the number of adult males of the red bearded winged turtle was counted in each trap. The test was conducted twice from August 30 to September 23, 2002 and from October 7 to October 31, 2002.
As a result, the number of killings in each section is shown in Table 1.

  In Comparative Example 1 and Comparative Example 2 in which no disturbing agent treatment is performed, 150 or more male adults are attracted in 3 weeks. However, in Example 1 and Example 2 in which the disturbing agent was treated, the attracting argument decreased by 70% or more, and it can be seen that a clear communication disturbing phenomenon occurred.

Examples 3-4 and Comparative Examples 3-4
Next, when 10 virgin females within 5 days after emergence are placed on the central laver trap as the attractant, and 10 mg of communication disrupter is treated at the four corners of the 5 m square (total of 4), and Table 2 shows the results of the communication disruption test when 10 mg of the communication disruptor was processed in a lattice pattern at intervals of 1 m in a 5 m square section (a total of 36).

When a synthetic pheromone was used as the attracting source, 4 attractants had a sufficient attracting inhibitory effect. However, when 10 virgin females were used as the attracting source, the attracting argument was higher than that of Comparative Example 3. Although a decrease was observed, the effect was not sufficient (Example 3).
However, when the number of disturbing agents is increased to 36 (Example 4), only two animals are attracted in one month, and it can be seen that the sex pheromone communication between males and females is disturbed very efficiently.

Claims (2)

  A weight ratio of n-hexyl n-hexylate, n-hexyl n-hexanoate (E) -2-hexenyl, and n-octyl n-butyrate, which is a sex pheromone of the red beetle moss turtle, 1000: (400 to 500): (10 to 100) A communication disturbing agent for red-bellied flies.   A method for disrupting the communication of the red-bellied squirrel turtle using the communication disrupter for red-skinned turtles according to claim 1.