JP2004534526A - 細胞内細菌からのタンパク質を同定する方法 - Google Patents
細胞内細菌からのタンパク質を同定する方法 Download PDFInfo
- Publication number
- JP2004534526A JP2004534526A JP2002579810A JP2002579810A JP2004534526A JP 2004534526 A JP2004534526 A JP 2004534526A JP 2002579810 A JP2002579810 A JP 2002579810A JP 2002579810 A JP2002579810 A JP 2002579810A JP 2004534526 A JP2004534526 A JP 2004534526A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- gene name
- proteins
- chlamydia
- trachomatis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 532
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 407
- 241000894006 Bacteria Species 0.000 title claims abstract description 109
- 238000000034 method Methods 0.000 title claims abstract description 97
- 230000003834 intracellular effect Effects 0.000 title claims abstract description 75
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 117
- 239000012634 fragment Substances 0.000 claims abstract description 54
- 230000002163 immunogen Effects 0.000 claims abstract description 52
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract description 33
- -1 nucleic acid compounds Chemical class 0.000 claims abstract description 27
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 26
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 16
- 235000018102 proteins Nutrition 0.000 claims description 397
- 210000004027 cell Anatomy 0.000 claims description 216
- 208000015181 infectious disease Diseases 0.000 claims description 74
- 241000606161 Chlamydia Species 0.000 claims description 73
- 239000012130 whole-cell lysate Substances 0.000 claims description 52
- 238000002372 labelling Methods 0.000 claims description 48
- 229940079156 Proteasome inhibitor Drugs 0.000 claims description 44
- 150000001413 amino acids Chemical class 0.000 claims description 44
- 239000003207 proteasome inhibitor Substances 0.000 claims description 44
- 241000606153 Chlamydia trachomatis Species 0.000 claims description 41
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 41
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 claims description 37
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 claims description 37
- 108091054437 MHC class I family Proteins 0.000 claims description 27
- 239000000427 antigen Substances 0.000 claims description 27
- 235000001014 amino acid Nutrition 0.000 claims description 26
- 108091007433 antigens Proteins 0.000 claims description 25
- 102000036639 antigens Human genes 0.000 claims description 25
- 241001647372 Chlamydia pneumoniae Species 0.000 claims description 22
- 102000043129 MHC class I family Human genes 0.000 claims description 22
- 229940038705 chlamydia trachomatis Drugs 0.000 claims description 20
- 229960005486 vaccine Drugs 0.000 claims description 20
- 238000011161 development Methods 0.000 claims description 18
- 239000013598 vector Substances 0.000 claims description 17
- 230000002829 reductive effect Effects 0.000 claims description 16
- 238000004949 mass spectrometry Methods 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- 238000000539 two dimensional gel electrophoresis Methods 0.000 claims description 11
- 241001465754 Metazoa Species 0.000 claims description 10
- 238000001437 electrospray ionisation time-of-flight quadrupole detection Methods 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 108020001507 fusion proteins Proteins 0.000 claims description 8
- 102000037865 fusion proteins Human genes 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- XUJNEKJLAYXESH-UHFFFAOYSA-N Cysteine Chemical compound SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 6
- 230000028993 immune response Effects 0.000 claims description 6
- 238000003119 immunoblot Methods 0.000 claims description 6
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 108090000631 Trypsin Proteins 0.000 claims description 5
- 102000004142 Trypsin Human genes 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 238000003114 enzyme-linked immunosorbent spot assay Methods 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 5
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 claims description 5
- 230000002285 radioactive effect Effects 0.000 claims description 5
- 239000012588 trypsin Substances 0.000 claims description 5
- 241000023308 Acca Species 0.000 claims description 4
- 101000621943 Acholeplasma phage L2 Probable integrase/recombinase Proteins 0.000 claims description 4
- 101000618348 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) Uncharacterized protein Alvin_0065 Proteins 0.000 claims description 4
- 101000781117 Autographa californica nuclear polyhedrosis virus Uncharacterized 12.4 kDa protein in CTL-LEF2 intergenic region Proteins 0.000 claims description 4
- 101000708323 Azospirillum brasilense Uncharacterized 28.8 kDa protein in nifR3-like 5'region Proteins 0.000 claims description 4
- 101000770311 Azotobacter chroococcum mcd 1 Uncharacterized 19.8 kDa protein in nifW 5'region Proteins 0.000 claims description 4
- 101000748761 Bacillus subtilis (strain 168) Uncharacterized MFS-type transporter YcxA Proteins 0.000 claims description 4
- 101000765620 Bacillus subtilis (strain 168) Uncharacterized protein YlxP Proteins 0.000 claims description 4
- 101000916134 Bacillus subtilis (strain 168) Uncharacterized protein YqxJ Proteins 0.000 claims description 4
- 101000754349 Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) UPF0065 protein BP0148 Proteins 0.000 claims description 4
- 101000827633 Caldicellulosiruptor sp. (strain Rt8B.4) Uncharacterized 23.9 kDa protein in xynA 3'region Proteins 0.000 claims description 4
- 101000947628 Claviceps purpurea Uncharacterized 11.8 kDa protein Proteins 0.000 claims description 4
- 101000686796 Clostridium perfringens Replication protein Proteins 0.000 claims description 4
- 102100039203 Cytochrome P450 3A7 Human genes 0.000 claims description 4
- 101000788129 Escherichia coli Uncharacterized protein in sul1 3'region Proteins 0.000 claims description 4
- 101100540144 Escherichia phage 186 apl gene Proteins 0.000 claims description 4
- 101000788370 Escherichia phage P2 Uncharacterized 12.9 kDa protein in GpA 3'region Proteins 0.000 claims description 4
- 101000787096 Geobacillus stearothermophilus Uncharacterized protein in gldA 3'region Proteins 0.000 claims description 4
- 101000976889 Haemophilus phage HP1 (strain HP1c1) Uncharacterized 19.2 kDa protein in cox-rep intergenic region Proteins 0.000 claims description 4
- 101000745715 Homo sapiens Cytochrome P450 3A7 Proteins 0.000 claims description 4
- 101000827627 Klebsiella pneumoniae Putative low molecular weight protein-tyrosine-phosphatase Proteins 0.000 claims description 4
- 101001130841 Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) Non-structural protein ORF5 Proteins 0.000 claims description 4
- 102100035832 Phakinin Human genes 0.000 claims description 4
- 108090000709 Phakinin Proteins 0.000 claims description 4
- 206010035664 Pneumonia Diseases 0.000 claims description 4
- 101000974028 Rhizobium leguminosarum bv. viciae (strain 3841) Putative cystathionine beta-lyase Proteins 0.000 claims description 4
- 101000756519 Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) Uncharacterized protein RCAP_rcc00048 Proteins 0.000 claims description 4
- 101000948219 Rhodococcus erythropolis Uncharacterized 11.5 kDa protein in thcD 3'region Proteins 0.000 claims description 4
- 101000984442 Sorghum bicolor Phosphoenolpyruvate carboxylase 3 Proteins 0.000 claims description 4
- 101000936711 Streptococcus gordonii Accessory secretory protein Asp4 Proteins 0.000 claims description 4
- 101000929863 Streptomyces cinnamonensis Monensin polyketide synthase putative ketoacyl reductase Proteins 0.000 claims description 4
- 101000788468 Streptomyces coelicolor Uncharacterized protein in mprR 3'region Proteins 0.000 claims description 4
- 101000845085 Streptomyces violaceoruber Granaticin polyketide synthase putative ketoacyl reductase 1 Proteins 0.000 claims description 4
- 101000711771 Thiocystis violacea Uncharacterized 76.5 kDa protein in phbC 3'region Proteins 0.000 claims description 4
- 101000711318 Vibrio alginolyticus Uncharacterized 11.6 kDa protein in scrR 3'region Proteins 0.000 claims description 4
- 101150019250 dksA gene Proteins 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 239000008194 pharmaceutical composition Substances 0.000 claims description 4
- 238000000159 protein binding assay Methods 0.000 claims description 4
- 238000011510 Elispot assay Methods 0.000 claims description 3
- 238000011887 Necropsy Methods 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 3
- 238000000132 electrospray ionisation Methods 0.000 claims description 3
- 101150093674 lpxD gene Proteins 0.000 claims description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 230000001131 transforming effect Effects 0.000 claims description 3
- 108090000317 Chymotrypsin Proteins 0.000 claims description 2
- 101100278012 Escherichia coli (strain K12) dnaG gene Proteins 0.000 claims description 2
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims description 2
- 102000043131 MHC class II family Human genes 0.000 claims description 2
- 108091054438 MHC class II family Proteins 0.000 claims description 2
- 101100203658 Mus musculus Spag1 gene Proteins 0.000 claims description 2
- 101100006766 Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) clpX gene Proteins 0.000 claims description 2
- 108090000284 Pepsin A Proteins 0.000 claims description 2
- 102000057297 Pepsin A Human genes 0.000 claims description 2
- 101100165173 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) basS gene Proteins 0.000 claims description 2
- 101100421924 Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) spo0C gene Proteins 0.000 claims description 2
- 229960002376 chymotrypsin Drugs 0.000 claims description 2
- 101150113622 clpC gene Proteins 0.000 claims description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 claims description 2
- 101150004979 flmA gene Proteins 0.000 claims description 2
- 101150054895 ftsH gene Proteins 0.000 claims description 2
- 230000009149 molecular binding Effects 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 101150048892 parB gene Proteins 0.000 claims description 2
- 229940111202 pepsin Drugs 0.000 claims description 2
- 101150079601 recA gene Proteins 0.000 claims description 2
- 101150049069 rpsM gene Proteins 0.000 claims description 2
- 238000001269 time-of-flight mass spectrometry Methods 0.000 claims description 2
- 101100492609 Talaromyces wortmannii astC gene Proteins 0.000 claims 1
- 101150116772 aatA gene Proteins 0.000 claims 1
- 101150005925 aspC gene Proteins 0.000 claims 1
- 238000005520 cutting process Methods 0.000 claims 1
- 101150100742 dapL gene Proteins 0.000 claims 1
- 238000006911 enzymatic reaction Methods 0.000 claims 1
- 101150037704 rplJ gene Proteins 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 abstract description 12
- 230000037361 pathway Effects 0.000 abstract description 3
- 239000000499 gel Substances 0.000 description 80
- 229940125575 vaccine candidate Drugs 0.000 description 60
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 23
- 230000007306 turnover Effects 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 18
- 239000006166 lysate Substances 0.000 description 16
- 230000015556 catabolic process Effects 0.000 description 15
- 238000006731 degradation reaction Methods 0.000 description 15
- 230000000694 effects Effects 0.000 description 14
- 210000000805 cytoplasm Anatomy 0.000 description 13
- 238000001906 matrix-assisted laser desorption--ionisation mass spectrometry Methods 0.000 description 13
- 230000028327 secretion Effects 0.000 description 13
- 108700026244 Open Reading Frames Proteins 0.000 description 12
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 12
- 238000013459 approach Methods 0.000 description 11
- 108010077805 Bacterial Proteins Proteins 0.000 description 10
- 150000002500 ions Chemical class 0.000 description 10
- 238000002965 ELISA Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 210000003000 inclusion body Anatomy 0.000 description 9
- 239000003112 inhibitor Substances 0.000 description 9
- 239000012139 lysis buffer Substances 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 8
- 238000003776 cleavage reaction Methods 0.000 description 8
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 8
- 239000012636 effector Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 210000004898 n-terminal fragment Anatomy 0.000 description 8
- 230000007017 scission Effects 0.000 description 8
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 7
- 108010029485 Protein Isoforms Proteins 0.000 description 7
- 102000001708 Protein Isoforms Human genes 0.000 description 7
- 239000002671 adjuvant Substances 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 6
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 6
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 6
- 238000000376 autoradiography Methods 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 210000004900 c-terminal fragment Anatomy 0.000 description 6
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 6
- 230000004960 subcellular localization Effects 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 5
- 238000013528 artificial neural network Methods 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 5
- 230000036425 denaturation Effects 0.000 description 5
- 238000001962 electrophoresis Methods 0.000 description 5
- 210000003527 eukaryotic cell Anatomy 0.000 description 5
- 238000013467 fragmentation Methods 0.000 description 5
- 238000006062 fragmentation reaction Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 238000010820 immunofluorescence microscopy Methods 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 5
- 238000001243 protein synthesis Methods 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- 229960004799 tryptophan Drugs 0.000 description 5
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 4
- 241000283707 Capra Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 102100036242 HLA class II histocompatibility antigen, DQ alpha 2 chain Human genes 0.000 description 4
- 101000930801 Homo sapiens HLA class II histocompatibility antigen, DQ alpha 2 chain Proteins 0.000 description 4
- 101710164702 Major outer membrane protein Proteins 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 239000002033 PVDF binder Substances 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 4
- 239000013592 cell lysate Substances 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000000562 conjugate Substances 0.000 description 4
- 235000018417 cysteine Nutrition 0.000 description 4
- 231100000433 cytotoxic Toxicity 0.000 description 4
- 230000001472 cytotoxic effect Effects 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 4
- 230000002035 prolonged effect Effects 0.000 description 4
- 230000017854 proteolysis Effects 0.000 description 4
- 230000002797 proteolythic effect Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 208000007190 Chlamydia Infections Diseases 0.000 description 3
- 206010061041 Chlamydial infection Diseases 0.000 description 3
- 102100038385 Coiled-coil domain-containing protein R3HCC1L Human genes 0.000 description 3
- 241000032203 Conus aureus Species 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 229930182566 Gentamicin Natural products 0.000 description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 3
- 101000743767 Homo sapiens Coiled-coil domain-containing protein R3HCC1L Proteins 0.000 description 3
- 102100037850 Interferon gamma Human genes 0.000 description 3
- 108010074328 Interferon-gamma Proteins 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 239000012980 RPMI-1640 medium Substances 0.000 description 3
- 241000607142 Salmonella Species 0.000 description 3
- 108091058545 Secretory proteins Proteins 0.000 description 3
- 102000040739 Secretory proteins Human genes 0.000 description 3
- 241000607768 Shigella Species 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000004721 adaptive immunity Effects 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000008045 co-localization Effects 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000002298 density-gradient ultracentrifugation Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 3
- 229960002518 gentamicin Drugs 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000004844 protein turnover Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 238000010600 3H thymidine incorporation assay Methods 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 108010078959 C-terminal processing peptidase Proteins 0.000 description 2
- 201000005019 Chlamydia pneumonia Diseases 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- 108010041986 DNA Vaccines Proteins 0.000 description 2
- 229940021995 DNA vaccine Drugs 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 102000025850 HLA-A2 Antigen Human genes 0.000 description 2
- 108010074032 HLA-A2 Antigen Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 108090000144 Human Proteins Proteins 0.000 description 2
- 102000003839 Human Proteins Human genes 0.000 description 2
- 241000186781 Listeria Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 108010026552 Proteome Proteins 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 108010075344 Tryptophan synthase Proteins 0.000 description 2
- 102000004243 Tubulin Human genes 0.000 description 2
- 108090000704 Tubulin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000030741 antigen processing and presentation Effects 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 229910052804 chromium Inorganic materials 0.000 description 2
- 239000011651 chromium Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 244000052637 human pathogen Species 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 208000030773 pneumonia caused by chlamydia Diseases 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 108020003519 protein disulfide isomerase Proteins 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 238000004885 tandem mass spectrometry Methods 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 108010087967 type I signal peptidase Proteins 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- LEVWYRKDKASIDU-OLPSTFOWSA-N (2R)-2-amino-3-[[(2R)-2-amino-2-carboxyethyl]di(35S)sulfanyl]propanoic acid Chemical compound C([C@@H](C(=O)O)N)[35S]SC[C@@H](C(=O)O)N LEVWYRKDKASIDU-OLPSTFOWSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- FFEARJCKVFRZRR-FOEKBKJKSA-N 3654-96-4 Chemical compound C[35S]CC[C@H](N)C(O)=O FFEARJCKVFRZRR-FOEKBKJKSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 102100038222 60 kDa heat shock protein, mitochondrial Human genes 0.000 description 1
- 101710154868 60 kDa heat shock protein, mitochondrial Proteins 0.000 description 1
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000607528 Aeromonas hydrophila Species 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 101100019907 Arabidopsis thaliana KDSA2 gene Proteins 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108090000549 Calreticulin Proteins 0.000 description 1
- 102100029968 Calreticulin Human genes 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 description 1
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 description 1
- 241000183712 Cerinthe major Species 0.000 description 1
- 241001674218 Chlamydia pecorum Species 0.000 description 1
- 241000606069 Chlamydiaceae Species 0.000 description 1
- 241000498849 Chlamydiales Species 0.000 description 1
- 241000201562 Chlamydophila pneumoniae AR39 Species 0.000 description 1
- 241000989743 Chlamydophila pneumoniae J138 Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 102100031375 Endothelial lipase Human genes 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 101100473116 Escherichia phage 186 CII gene Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 101000941275 Homo sapiens Endothelial lipase Proteins 0.000 description 1
- 101000959820 Homo sapiens Interferon alpha-1/13 Proteins 0.000 description 1
- 101001136981 Homo sapiens Proteasome subunit beta type-9 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 101150106931 IFNG gene Proteins 0.000 description 1
- 241000976924 Inca Species 0.000 description 1
- 108010065805 Interleukin-12 Proteins 0.000 description 1
- 102000013462 Interleukin-12 Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- 125000002842 L-seryl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])O[H] 0.000 description 1
- 241000589248 Legionella Species 0.000 description 1
- 208000007764 Legionnaires' Disease Diseases 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 1
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 101100462201 Mus musculus Opn4 gene Proteins 0.000 description 1
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 1
- 208000022873 Ocular disease Diseases 0.000 description 1
- 101710098399 Outer membrane protein YopN Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108700020474 Penicillin-Binding Proteins Proteins 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010026809 Peptide deformylase Proteins 0.000 description 1
- 102100021418 Peptide deformylase, mitochondrial Human genes 0.000 description 1
- 108010090127 Periplasmic Proteins Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 108091036414 Polyinosinic:polycytidylic acid Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010030304 Progesterone-Binding Globulin Proteins 0.000 description 1
- 102100031300 Proteasome activator complex subunit 1 Human genes 0.000 description 1
- 101710103872 Proteasome activator complex subunit 1 Proteins 0.000 description 1
- 102100035764 Proteasome subunit beta type-9 Human genes 0.000 description 1
- 241000287531 Psittacidae Species 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 101710137010 Retinol-binding protein 3 Proteins 0.000 description 1
- 102100038247 Retinol-binding protein 3 Human genes 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 101100324158 Salmonella typhi aroA gene Proteins 0.000 description 1
- 208000007893 Salpingitis Diseases 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 101710127774 Stress response protein Proteins 0.000 description 1
- 108010008038 Synthetic Vaccines Proteins 0.000 description 1
- 230000006052 T cell proliferation Effects 0.000 description 1
- 102100036407 Thioredoxin Human genes 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000003092 anti-cytokine Effects 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 201000000902 chlamydia Diseases 0.000 description 1
- 208000012538 chlamydia trachomatis infectious disease Diseases 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000037012 chymotrypsin-like activity Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 238000012303 cytoplasmic staining Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 238000002784 cytotoxicity assay Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- AFOSIXZFDONLBT-UHFFFAOYSA-N divinyl sulfone Chemical compound C=CS(=O)(=O)C=C AFOSIXZFDONLBT-UHFFFAOYSA-N 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 201000003511 ectopic pregnancy Diseases 0.000 description 1
- 238000001425 electrospray ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 201000010063 epididymitis Diseases 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 229940009493 gel-one Drugs 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000008348 humoral response Effects 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 230000008073 immune recognition Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 201000001371 inclusion conjunctivitis Diseases 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 108020004201 indoleamine 2,3-dioxygenase Proteins 0.000 description 1
- 102000006639 indoleamine 2,3-dioxygenase Human genes 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 210000005061 intracellular organelle Anatomy 0.000 description 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 101150049174 kdsA gene Proteins 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007347 lysosomal proteolysis Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000000863 peptide conjugate Substances 0.000 description 1
- 238000012510 peptide mapping method Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 108091008695 photoreceptors Proteins 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000012018 process simulation test Methods 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 238000000575 proteomic method Methods 0.000 description 1
- 230000004063 proteosomal degradation Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 229940124551 recombinant vaccine Drugs 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 208000020029 respiratory tract infectious disease Diseases 0.000 description 1
- 230000017702 response to host Effects 0.000 description 1
- 102000029752 retinol binding Human genes 0.000 description 1
- 108091000053 retinol binding Proteins 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 150000003839 salts Chemical group 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 201000000306 sarcoidosis Diseases 0.000 description 1
- 238000003345 scintillation counting Methods 0.000 description 1
- 101150008186 sctN gene Proteins 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 229940094937 thioredoxin Drugs 0.000 description 1
- 150000004992 toluidines Chemical class 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 206010044325 trachoma Diseases 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 108091008023 transcriptional regulators Proteins 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 101150019416 trpA gene Proteins 0.000 description 1
- 230000001810 trypsinlike Effects 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 208000000143 urethritis Diseases 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/295—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Chlamydiales (O)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Reproductive Health (AREA)
- General Engineering & Computer Science (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Ophthalmology & Optometry (AREA)
- Bioinformatics & Computational Biology (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28251301P | 2001-04-09 | 2001-04-09 | |
DKPA200100581 | 2001-04-09 | ||
PCT/DK2002/000234 WO2002082091A2 (en) | 2001-04-09 | 2002-04-09 | Method for identification of proteins from intracellular bacteria |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2004534526A true JP2004534526A (ja) | 2004-11-18 |
JP2004534526A5 JP2004534526A5 (xx) | 2006-01-05 |
Family
ID=26069005
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002579810A Pending JP2004534526A (ja) | 2001-04-09 | 2002-04-09 | 細胞内細菌からのタンパク質を同定する方法 |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP1412757A2 (xx) |
JP (1) | JP2004534526A (xx) |
KR (1) | KR20040002905A (xx) |
CN (1) | CN1531653A (xx) |
BR (1) | BR0208786A (xx) |
CA (1) | CA2443813A1 (xx) |
IL (1) | IL158136A0 (xx) |
MX (1) | MXPA03009222A (xx) |
WO (1) | WO2002082091A2 (xx) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2020510829A (ja) * | 2017-03-02 | 2020-04-09 | ビオスパーク・ベー・フェーBiosparq B.V. | 診断方法および診断システム |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9828000D0 (en) | 1998-12-18 | 1999-02-10 | Chiron Spa | Antigens |
US7576206B2 (en) | 2003-08-14 | 2009-08-18 | Cephalon, Inc. | Proteasome inhibitors and methods of using the same |
US7223745B2 (en) | 2003-08-14 | 2007-05-29 | Cephalon, Inc. | Proteasome inhibitors and methods of using the same |
GB0409748D0 (en) * | 2004-04-30 | 2004-06-09 | Chiron Srl | Lactoferrin cleavage |
US7468383B2 (en) | 2005-02-11 | 2008-12-23 | Cephalon, Inc. | Proteasome inhibitors and methods of using the same |
EP2152303A4 (en) | 2007-05-01 | 2011-10-05 | Univ Texas | CHLAMYDIA ANTIGENS AS REAGENTS FOR THE DIAGNOSIS AND TREATMENT OF CHLAMYDIA INFECTION AND DISEASE |
US7892567B2 (en) | 2007-10-01 | 2011-02-22 | Board Of Regents, The University Of Texas System | Methods and compositions for immunization against chlamydial infection and disease |
JP2012505212A (ja) | 2008-10-09 | 2012-03-01 | ボード・オヴ・リージェンツ,ユニヴァーシティ・オヴ・テキサス・システム | クラミジア感染および疾患の診断および治療のためのクラミジア抗原に関する方法および組成物 |
AU2010341530B2 (en) | 2009-12-22 | 2016-03-10 | Cephalon, Inc. | Proteasome inhibitors and processes for their preparation, purification and use |
CN102749233A (zh) * | 2012-06-04 | 2012-10-24 | 王新华 | 一种maldi-tof ms直接检测感染尿液病原菌前处理方法 |
CN112175955B (zh) * | 2020-10-29 | 2022-05-24 | 西南大学 | 一种在植物花粉中特异表达的强启动子cp09及其应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001515064A (ja) * | 1997-08-15 | 2001-09-18 | フランソワ・スーシー | クラスト−ラクタシスチンβ−ラクトンおよびそのアナログの合成 |
KR20010032336A (ko) * | 1997-11-21 | 2001-04-16 | 브랑디 빠스깔 | 클라미디아 뉴모니아 게놈 서열과 폴리펩티드, 이의 단편및 이의 용도, 특히, 감염의 진단, 예방 및 치료 용도 |
KR100769103B1 (ko) * | 1997-11-28 | 2007-10-23 | 세로노 제네틱스 인스티튜트 에스.에이. | 클라미디아 트라코마티스 게놈 서열과 폴리펩티드, 이의단편 및 이의 용도, 특히, 감염의 진단, 예방 및 치료 용도 |
-
2002
- 2002-04-09 CA CA002443813A patent/CA2443813A1/en not_active Abandoned
- 2002-04-09 JP JP2002579810A patent/JP2004534526A/ja active Pending
- 2002-04-09 MX MXPA03009222A patent/MXPA03009222A/es unknown
- 2002-04-09 KR KR10-2003-7013236A patent/KR20040002905A/ko not_active Application Discontinuation
- 2002-04-09 WO PCT/DK2002/000234 patent/WO2002082091A2/en not_active Application Discontinuation
- 2002-04-09 EP EP02759766A patent/EP1412757A2/en not_active Withdrawn
- 2002-04-09 BR BR0208786-3A patent/BR0208786A/pt not_active IP Right Cessation
- 2002-04-09 IL IL15813602A patent/IL158136A0/xx unknown
- 2002-04-09 CN CNA028091140A patent/CN1531653A/zh active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2020510829A (ja) * | 2017-03-02 | 2020-04-09 | ビオスパーク・ベー・フェーBiosparq B.V. | 診断方法および診断システム |
Also Published As
Publication number | Publication date |
---|---|
IL158136A0 (en) | 2004-03-28 |
MXPA03009222A (es) | 2004-11-12 |
EP1412757A2 (en) | 2004-04-28 |
CN1531653A (zh) | 2004-09-22 |
WO2002082091A3 (en) | 2004-03-04 |
BR0208786A (pt) | 2004-03-09 |
KR20040002905A (ko) | 2004-01-07 |
CA2443813A1 (en) | 2002-10-17 |
WO2002082091A2 (en) | 2002-10-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Vandahl et al. | Proteome analysis of the Chlamydia pneumoniaeelementary body | |
Fields et al. | Chlamydia trachomatis type III secretion: evidence for a functional apparatus during early‐cycle development | |
Wang et al. | A genome-wide profiling of the humoral immune response to Chlamydia trachomatis infection reveals vaccine candidate antigens expressed in humans | |
US20050239160A1 (en) | Method for identification of proteins from intracellular bacteria | |
Montigiani et al. | Genomic approach for analysis of surface proteins in Chlamydia pneumoniae | |
Hoelzle et al. | First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1) | |
US20080226679A1 (en) | Novel surface exposed proteins from chlamydia pneumoniae | |
US20090011426A1 (en) | Methods of Diagnosis and Treatment of M.Tuberculosis Infection and Reagents Therefor | |
JP2004534526A (ja) | 細胞内細菌からのタンパク質を同定する方法 | |
JP2004534526A5 (xx) | ||
Tanzer et al. | Identification of polymorphic outer membrane proteins of Chlamydia psittaci 6BC | |
Dechamma et al. | Identification of T-helper and linear B epitope in the hypervariable region of nucleocapsid protein of PPRV and its use in the development of specific antibodies to detect viral antigen | |
Finco et al. | Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings | |
US7105170B2 (en) | Latent human tuberculosis model, diagnostic antigens, and methods of use | |
Tomson et al. | Assessment of cell-surface exposure and vaccinogenic potentials of Treponema pallidum candidate outer membrane proteins | |
Faria et al. | Immunoproteomics of Brucella abortus reveals potential of recombinant antigens for discriminating vaccinated from naturally infected cattle | |
AU2008246064A1 (en) | Chlamydial antigens as reagents for diagnosis and treatment of chlamydial infection and disease | |
Vandahl et al. | Secretion of Cpn0796 from Chlamydia pneumoniae into the host cell cytoplasm by an autotransporter mechanism | |
Wu et al. | Proteomic identification of immunodominant membrane-related antigens in Campylobacter jejuni associated with sheep abortion | |
US20110212133A1 (en) | Novel Methods of Diagnosis and Treatment of P. Aeruginosa Infection and Reagents Therefor | |
Luo et al. | Localization of the hypothetical protein Cpn0585 in the inclusion membrane of Chlamydia pneumoniae-infected cells | |
AU2002338351A1 (en) | Method for identification of proteins from intracellular bacteria | |
Shabani et al. | Mass spectrometry-based immunopeptidomics and computational vaccinology strategies for the identification of universal Shigella immunogenic candidates | |
Birkelund et al. | Characterization of a linear epitope on Chlamydia trachomatis serovar L2 DnaK-like protein | |
DelVecchio et al. | Proteomics for the development of vaccines and therapeutics |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050210 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050216 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050317 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20080125 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20080704 |