JP2004517603A5 - - Google Patents
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- JP2004517603A5 JP2004517603A5 JP2001538960A JP2001538960A JP2004517603A5 JP 2004517603 A5 JP2004517603 A5 JP 2004517603A5 JP 2001538960 A JP2001538960 A JP 2001538960A JP 2001538960 A JP2001538960 A JP 2001538960A JP 2004517603 A5 JP2004517603 A5 JP 2004517603A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- protein
- coupled receptor
- host cell
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000003688 G-protein coupled receptors Human genes 0.000 description 11
- 108090000045 G-protein coupled receptors Proteins 0.000 description 11
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 229920000023 polynucleotide Polymers 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920001850 Nucleic acid sequence Polymers 0.000 description 1
- 102000011587 Polyproteins Human genes 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 Ｇタンパク質共役型受容体（ＧＰＣＲ）をコードする核酸を含む単離されたポリヌクレオチドであって、該Ｇタンパク質共役型受容体は、配列番号２；配列番号４；配列番号６；配列番号８；配列番号１０；配列番号１２；配列番号１４；配列番号１６；配列番号１８；配列番号２０；配列番号２２；配列番号２４；配列番号２６；配列番号２８；配列番号３０；配列番号３２；配列番号３４；配列番号３６；配列番号３８；および配列番号４０からなる群より選択されるアミノ酸配列に対して少なくとも約８０％の同一性を有するアミノ酸配列を含む、単離されたポリヌクレオチド。
【請求項２】 請求項１に記載の単離されたポリヌクレオチドであって、前記核酸は、配列番号１；配列番号３；配列番号５；配列番号７；配列番号９；配列番号１１；配列番号１３；配列番号１５；配列番号１７；配列番号１９；配列番号２１；配列番号２３；配列番号２５；配列番号２７；配列番号２９；配列番号３１；配列番号３３；配列番号３５；配列番号３７；および配列番号３９からなる群より選択されるヌクレオチド配列を含む、単離されたポリヌクレオチド。
【請求項３】 請求項１に記載のポリヌクレオチドを含む、ベクター。
【請求項４】 請求項３に記載のベクターであって、該ベクターは、発現ベクターであり、前記ポリヌクレオチドは、プロモーターに作動可能に連結されている、ベクター。
【請求項５】 請求項３に記載のベクターを含む、組換え宿主細胞。
【請求項６】 請求項４に記載の発現ベクターを含む、組換え宿主細胞。
【請求項７】 組換え宿主細胞を作製するための方法であって、
（ａ）請求項４に記載の発現ベクターを適切な宿主細胞中に導入する工程；および
（ｂ）該宿主細胞を、該細胞による前記Ｇタンパク質共役型受容体の発現を可能にする条件下で培養する工程；
を包含する、方法。
【請求項８】 請求項７に記載の組換え宿主細胞の単離された膜であって、該単離された膜は、前記Ｇタンパク質共役型受容体を含む、膜。
【請求項９】 単離されたＧタンパク質共役型受容体または組換えＧタンパク質共役型受容体であって、該Ｇタンパク質共役型受容体は、配列番号２；配列番号４；配列番号６；配列番号８；配列番号１０；配列番号１２；配列番号１４；配列番号１６；配列番号１８；配列番号２０；配列番号２２；配列番号２４；配列番号２６；配列番号２８；配列番号３０；配列番号３２；配列番号３４；配列番号３６；配列番号３８；および配列番号４０からなる群より選択されるアミノ酸配列に対して少なくとも約８０％の同一性を有するアミノ酸配列を含む、Ｇタンパク質共役型受容体。
【請求項１０】 方法であって、
（ａ）候補化合物を、請求項９に記載のＧタンパク質共役型受容体を含む組換え宿主細胞またはその膜と接触させる工程；および
（ｂ）該化合物が該Ｇタンパク質共役型受容体を阻害または刺激する能力を測定する工程；
を包含する、方法。
[Claims]
1. An isolated polynucleotide comprising a nucleic acid encoding a G protein-coupled receptor (GPCR), wherein the G protein-coupled receptor is SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6. SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; SEQ ID NO: 22; SEQ ID NO: 24; SEQ ID NO: 26; SEQ ID NO: 32; SEQ ID NO: 36; SEQ ID NO: 38; and SEQ ID NO: 40, wherein the isolated polyprotein comprises an amino acid sequence having at least about 80% identity to an amino acid sequence selected from the group consisting of: nucleotide.
2. The isolated polynucleotide of claim 1, wherein said nucleic acid is SEQ ID NO: 1; SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO: 9; SEQ ID NO: 11; SEQ ID NO: 13; SEQ ID NO: 17; SEQ ID NO: 19; SEQ ID NO: 21; SEQ ID NO: 23; SEQ ID NO: 25; SEQ ID NO: 27; SEQ ID NO: 29; SEQ ID NO: 31; And a nucleotide sequence selected from the group consisting of SEQ ID NO: 39.
[3] A vector comprising the polynucleotide according to [1] .
4. The vector according to claim 3, wherein said vector is an expression vector and said polynucleotide is operably linked to a promoter.
5. A recombinant host cell comprising the vector according to claim 3.
6. A recombinant host cell comprising the expression vector according to claim 4.
7. A method for producing a recombinant host cell, comprising:
(A) introducing the expression vector according to claim 4 into a suitable host cell; and
(B) culturing the host cell under conditions that allow the cell to express the G protein-coupled receptor;
A method comprising:
8. An isolated membrane of the recombinant host cell according to claim 7, wherein the isolated membrane comprises the G protein-coupled receptor.
9. An isolated G protein-coupled receptor or a recombinant G protein-coupled receptor, wherein the G protein-coupled receptor is SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; SEQ ID NO: 22; SEQ ID NO: 24; A G protein-coupled receptor comprising an amino acid sequence having at least about 80% identity to an amino acid sequence selected from the group consisting of: SEQ ID NO: 34; SEQ ID NO: 36; SEQ ID NO: 38;
10. A method, comprising :
(A) contacting the candidate compound with a recombinant host cell comprising the G protein-coupled receptor according to claim 9 or a membrane thereof; and
(B) measuring the ability of the compound to inhibit or stimulate the G protein-coupled receptor;
A method comprising:
Applications Claiming Priority (45)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16636999P | 1999-11-17 | 1999-11-17 | |
| US16608899P | 1999-11-17 | 1999-11-17 | |
| US16609999P | 1999-11-17 | 1999-11-17 | |
| US60/166,088 | 1999-11-17 | ||
| US60/166,369 | 1999-11-17 | ||
| US60/166,099 | 1999-11-17 | ||
| US17190099P | 1999-12-23 | 1999-12-23 | |
| US17190299P | 1999-12-23 | 1999-12-23 | |
| US17190199P | 1999-12-23 | 1999-12-23 | |
| US60/171,900 | 1999-12-23 | ||
| US60/171,901 | 1999-12-23 | ||
| US60/171,902 | 1999-12-23 | ||
| US18174900P | 2000-02-11 | 2000-02-11 | |
| US60/181,749 | 2000-02-11 | ||
| US18925900P | 2000-03-14 | 2000-03-14 | |
| US18925800P | 2000-03-14 | 2000-03-14 | |
| US60/189,259 | 2000-03-14 | ||
| US60/189,258 | 2000-03-14 | ||
| US19589800P | 2000-04-10 | 2000-04-10 | |
| US19607800P | 2000-04-10 | 2000-04-10 | |
| US19589900P | 2000-04-10 | 2000-04-10 | |
| US60/196,078 | 2000-04-10 | ||
| US60/195,898 | 2000-04-10 | ||
| US60/195,899 | 2000-04-10 | ||
| US20041900P | 2000-04-28 | 2000-04-28 | |
| US60/200,419 | 2000-04-28 | ||
| US20363000P | 2000-05-12 | 2000-05-12 | |
| US60/203,630 | 2000-05-12 | ||
| US21098200P | 2000-06-12 | 2000-06-12 | |
| US21074100P | 2000-06-12 | 2000-06-12 | |
| US60/210,741 | 2000-06-12 | ||
| US60/210,982 | 2000-06-12 | ||
| US22676000P | 2000-08-21 | 2000-08-21 | |
| US60/226,760 | 2000-08-21 | ||
| US23541800P | 2000-09-26 | 2000-09-26 | |
| US23577900P | 2000-09-26 | 2000-09-26 | |
| US60/235,779 | 2000-09-26 | ||
| US60/235,418 | 2000-09-26 | ||
| US24233200P | 2000-10-20 | 2000-10-20 | |
| US24234300P | 2000-10-20 | 2000-10-20 | |
| US60/242,332 | 2000-10-20 | ||
| US60/242,343 | 2000-10-20 | ||
| US24301900P | 2000-10-24 | 2000-10-24 | |
| US60/243,019 | 2000-10-24 | ||
| PCT/US2000/031509 WO2001036471A2 (en) | 1999-11-17 | 2000-11-16 | Endogenous and non-endogenous versions of human g protein-coupled receptors |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010294149A Division JP2011097950A (en) | 1999-11-17 | 2010-12-28 | Endogenous and non-endogenous versions of human g protein-coupled receptor |
| JP2011105725A Division JP2011147461A (en) | 1999-11-17 | 2011-05-10 | Endogenous and non-endogenous version of human g protein-coupled receptor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004517603A JP2004517603A (en) | 2004-06-17 |
| JP2004517603A5 true JP2004517603A5 (en) | 2007-12-20 |
| JP4768946B2 JP4768946B2 (en) | 2011-09-07 |
Family
ID=44693672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001538960A Expired - Fee Related JP4768946B2 (en) | 1999-11-17 | 2000-11-16 | Endogenous and non-endogenous forms of the human G protein coupled receptor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4768946B2 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6555339B1 (en) * | 1997-04-14 | 2003-04-29 | Arena Pharmaceuticals, Inc. | Non-endogenous, constitutively activated human protein-coupled receptors |
| US5891720A (en) * | 1997-04-17 | 1999-04-06 | Millennium Pharmaceuticals, Inc. | Isolated DNA encoding a novel human G-protein coupled receptor |
-
2000
- 2000-11-16 JP JP2001538960A patent/JP4768946B2/en not_active Expired - Fee Related
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