JP2004511237A - 代謝生成物の蓄積のモジュレーターについてスクリーニングするための哺乳動物２−ハイブリッドシステム - Google Patents

代謝生成物の蓄積のモジュレーターについてスクリーニングするための哺乳動物２−ハイブリッドシステム Download PDF

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JP2004511237A
JP2004511237A JP2002534565A JP2002534565A JP2004511237A JP 2004511237 A JP2004511237 A JP 2004511237A JP 2002534565 A JP2002534565 A JP 2002534565A JP 2002534565 A JP2002534565 A JP 2002534565A JP 2004511237 A JP2004511237 A JP 2004511237A
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nucleic acid
cell
protein
chimeric protein
binds
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Japanese (ja)
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JP2004511237A5 (enExample
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鉄　治
脇田　賢一
フランク・マコーミック
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University of California
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University of California San Diego UCSD
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Publication of JP2004511237A publication Critical patent/JP2004511237A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0058—Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2002534565A 2000-10-13 2001-10-09 代謝生成物の蓄積のモジュレーターについてスクリーニングするための哺乳動物２−ハイブリッドシステム Pending JP2004511237A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/687,593 US6787321B1 (en) 2000-10-13 2000-10-13 Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
PCT/US2001/031745 WO2002031201A2 (en) 2000-10-13 2001-10-09 Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products

Publications (2)

Publication Number Publication Date
JP2004511237A true JP2004511237A (ja) 2004-04-15
JP2004511237A5 JP2004511237A5 (enExample) 2009-01-29

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Application Number Title Priority Date Filing Date
JP2002534565A Pending JP2004511237A (ja) 2000-10-13 2001-10-09 代謝生成物の蓄積のモジュレーターについてスクリーニングするための哺乳動物２−ハイブリッドシステム

Country Status (7)

Country Link
US (2) US6787321B1 (enExample)
EP (1) EP1327003B1 (enExample)
JP (1) JP2004511237A (enExample)
AT (1) ATE520788T1 (enExample)
AU (1) AU2001296805A1 (enExample)
CA (1) CA2424317A1 (enExample)
WO (1) WO2002031201A2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
AU2006247234A1 (en) * 2005-05-19 2006-11-23 Wyeth Method of identifying compounds that modulate interaction of androgen receptor with beta-catenin
US20100099096A1 (en) * 2007-02-28 2010-04-22 The Brigham And Women's Hospital, Inc. Compositions and Methods for Identifying Factors Affecting Protein Stability
WO2009007980A1 (en) * 2007-07-11 2009-01-15 Yeda Research And Development Co. Ltd. Nucleic acid construct systems capable of diagnosing or treating a cell state
US20120156180A1 (en) * 2009-08-10 2012-06-21 The Board Of Trustees Of The University Of Illinois Compositions and methods for the treatment of krabbe and other neurodegenerative diseases
GB2584211B (en) * 2017-11-16 2023-05-24 Synthex Inc Selective modulation of protein-protein interactions
AU2020275425B2 (en) 2019-05-15 2024-06-27 Synthex, Inc. Selective degradation of proteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0370813A3 (en) 1988-11-25 1991-06-19 Exemplar Corporation Rapid screening mutagenesis and teratogenesis assay
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5525490A (en) 1994-03-29 1996-06-11 Onyx Pharmaceuticals, Inc. Reverse two-hybrid method
CA2217545A1 (en) 1995-04-11 1996-10-17 Marc Vidal Reverse two-hybrid systems
FR2734567B1 (fr) 1995-05-26 1997-08-08 Commissariat Energie Atomique Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines
USH1892H (en) 1995-07-27 2000-10-03 Pharmacia And Upjohn Company High volume nutrient based yeast two-hybrid assay for the identification of specific protein:protein interacting inhibitors
US5948620A (en) 1996-08-05 1999-09-07 Amersham International Plc Reverse two-hybrid system employing post-translation signal modulation
US6133027A (en) 1996-08-07 2000-10-17 City Of Hope Inducible expression system
US6051381A (en) 1996-12-11 2000-04-18 Kornacker; Michael G. Prokaryotic two-hybrid system
US5851775A (en) * 1997-03-20 1998-12-22 Johns Hopkins University β-catenin, Tcf-4, and APC interact to prevent cancer
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions
US6242253B1 (en) * 1997-10-09 2001-06-05 Regents Of The University Of California IkB kinase, subunits thereof, and methods of using same

Also Published As

Publication number Publication date
AU2001296805A1 (en) 2002-04-22
US7358086B2 (en) 2008-04-15
ATE520788T1 (de) 2011-09-15
EP1327003A2 (en) 2003-07-16
WO2002031201A2 (en) 2002-04-18
WO2002031201A3 (en) 2002-11-21
CA2424317A1 (en) 2002-04-18
US20040265791A1 (en) 2004-12-30
EP1327003B1 (en) 2011-08-17
US6787321B1 (en) 2004-09-07

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