JP2004301625A - Biochemical reaction cartridge - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To make a reagent in a chamber of a solution storage part surely flow into a chamber of a reaction part. <P>SOLUTION: A valve rod 7 having a cutout 8 formed thereon is inserted into the chamber 6 of the solution storage part 2 containing a solution and held by two O-rings 9, and the lower part of the solution chamber 6 is blocked by an aluminium foil sheet 10. A liquid specimen such as blood is injected from a specimen inlet by an inspector, and the valve rod 7 is plunged by using a short press rod 13a of a tool needle 13 to break the aluminium foil sheet 10, and then the solution is moved from the chamber 6 into the chamber 11. Since the notch 8 is connected to the upper and lower parts of the two O-rings 9 in this state, the air passes between the chamber 6 and the outside, and thereby the solution is moved smoothly. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a biochemical reaction cartridge used by incorporating cells, microorganisms, chromosomes, nucleic acids, and the like in a sample into an apparatus for analyzing by utilizing a biochemical reaction such as an antigen-antibody reaction or a nucleic acid hybridization reaction. .
[0002]
[Prior art]
Many analyzers for specimens such as blood use an immunological method utilizing an antigen-body reaction or a method utilizing nucleic acid hybridization. As an example of such an analysis method, a protein such as an antibody or an antigen or a single-stranded nucleic acid that specifically binds to a substance to be detected is used as a probe and immobilized on a solid phase surface such as fine particles, beads, and a glass plate. Then, an antigen-antibody reaction or nucleic acid hybridization is performed with the substance to be detected.
[0003]
Then, using a labeling substance having a specific interaction carrying a labeling substance having a high detection sensitivity such as an enzyme, a fluorescent substance, and a luminescent substance, for example, a labeled antibody or a labeled antigen or a labeled nucleic acid, It detects antigen-antibody compounds and double-stranded nucleic acids to detect the presence or absence of a test substance or to quantify the test substance.
[0004]
As a development of these techniques, US Pat. No. 5,445,934 discloses a so-called DNA array in which a large number of DNA (deoxyribonucleic acid) probes having mutually different base sequences are arranged in an array on a substrate. Have been. Also, Anal. Biochem. 270 (1), 103-111 and 1999 disclose a method for preparing a protein array having a configuration such as a DNA array by arranging various types of proteins on a membrane filter. In addition, it has become possible to inspect a very large number of items at once by using a DNA array, a protein array, or the like.
[0005]
Also, in various sample analysis methods, disposable biochemical reaction cartridges that perform the necessary reactions internally have been proposed for the purpose of reducing contamination by the sample, increasing the efficiency of the reaction, miniaturizing the device, and simplifying the work. Japanese Patent Application Laid-Open No. 11-509094 discloses a method in which a plurality of chambers are arranged in a biochemical reaction cartridge including a DNA array, a solution is moved by a differential pressure, and extraction or amplification of DNA in a sample is performed inside the cartridge. A biochemical reaction cartridge that enables a reaction such as hybridization is disclosed.
[0006]
As a method for supplying a reagent in a biochemical reaction cartridge, Japanese Patent Application Laid-Open No. 2000-266759 discloses that a reagent is supplied from an external reagent bottle to a disposable analysis cassette, and JP-A-11-509094 is disclosed. Discloses that a reagent is previously incorporated in a chamber.
[0007]
[Problems to be solved by the invention]
However, when supplying reagents from the outside, it is necessary to prepare a plurality of reagents separately from the biochemical reaction cartridge, and if there are many test items, the types of reagents required are increased, and replenishment becomes complicated. In addition, there is a possibility that the type of the reagent is mistaken. In the case of a reagent containing a reagent in the chamber of a biochemical reaction cartridge, the reagent in the chamber flows into the flow path or other chambers due to environmental changes during storage / transport or vibrations during transport, and the intended reaction is not performed. Different reactions can occur.
[0008]
An object of the present invention is to solve the above-mentioned problems, troublesome replenishment of reagents, mistakes in the types of reagents, and allow the reagents in the chamber to flow through the flow path and other parts even when the environment changes or vibrations during storage and transport. It is an object of the present invention to provide a biochemical reaction cartridge that does not flow into a chamber.
[0009]
[Means for Solving the Problems]
In order to achieve the above object, a biochemical reaction cartridge according to the present invention comprises a reaction part including a chamber and a flow path, and a solution storage part which is separated from or separate from the reaction part and stores a solution at a position corresponding to the chamber. And a partition member that can penetrate between the solution storage part and the reaction part is provided to move the solution from the solution storage part to the chamber of the reaction part.
[0010]
BEST MODE FOR CARRYING OUT THE INVENTION
The present invention will be described in detail based on the illustrated embodiment.
FIG. 1 is a perspective view of a biochemical reaction cartridge according to the present embodiment. The cartridge has a two-layer structure including a reaction part 1 in which a reaction is performed, and a solution storage part 2 disposed on the reaction part 1 for storing a solution such as a reagent and a cleaning agent. The main body of the reaction part 1 and the solution storage part 2 is made of synthetic resin such as polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene (ABS) copolymer, polystyrene, polycarbonate, polyester, polyvinyl chloride and the like. I have. When performing an optical measurement on the reactant, the reaction part 1 requires a transparent or translucent plastic.
[0011]
A sample inlet 3 for injecting a sample such as blood using a syringe is provided at an upper portion of the reaction part 1, and the sample inlet 3 is sealed with a rubber cap. In addition, a plurality of nozzle inlets 4 are provided on both side surfaces of the reaction part 1 for inserting a nozzle and performing pressurization or depressurization in order to move the solution in the reaction part 1. Are sealed by a rubber cap, and the opposite surface has the same configuration.
[0012]
Further, on the upper part of the solution storage part 2, three aluminum foil sheets 5 for covering an upper part of a solution storage chamber described later are stuck. The reaction part 1 and the solution storage part 2 are welded by ultrasonic waves. In addition, the reaction part 1 and the solution storage part 2 may be configured separately, and may be overlapped at the time of use.
[0013]
FIG. 2 shows a plan cross-sectional view of the solution storage part 2 of FIG. 1, provided with independent chambers 6 a to 6 m containing a solution, wherein the chambers 6 a and 6 b each include a first hemolytic agent containing EDTA that breaks cell walls, A second hemolytic agent including a protein denaturant such as a surfactant is accumulated. In the chamber 6c, silica-coated magnetic particles to which DNA is adsorbed are accumulated. In the chambers 6l and 6m, first and second extraction detergents used for purifying DNA at the time of extracting DNA are used. Has been accumulated.
[0014]
The chamber 6d contains an eluate composed of a buffer of a low-concentration salt that elutes DNA from magnetic particles, and the chamber 6g contains a mixture of a primer, a polymerase, a dNTP solution, a buffer, and a Cy-3dUTP containing a fluorescent agent necessary for PCR. Is filled. In the chambers 6h and 6j, a detergent containing a surfactant for washing the unhybridized fluorescently labeled sample DNA and the fluorescent label is contained. In the chamber 6i, a chamber containing a DNA microarray to be described later is dried. Alcohol has accumulated. Each of the chambers 6a to 6m is provided with a sharp valve stem 7a to 7m for penetrating a seat to be described later.
[0015]
FIG. 3 is a cross-sectional view showing a state of the biochemical reaction cartridge during storage, in which a valve stem 7 having a notch 8 is inserted into a chamber 6 of a solution storage part 2 containing a solution, and two O-rings are provided. 9. The lower part of the solution chamber 6 is closed by an aluminum foil sheet 10, and a sealing material 12 is provided between the chamber 6 and the chamber 11 of the reaction part 1 to prevent air from entering and leaving. Changes in the volume and pressure of the solution and air due to the environment can be absorbed by deformation of the aluminum foil sheet 10, so that the solution in the chamber 6 does not inadvertently enter the reaction section 1.
[0016]
FIG. 4 shows a state in which the examiner injects a liquid sample such as blood from the sample inlet 3 and sets it in a processing device described later, and then uses the short pressing rod 13 a of the tool needle 13 to move the valve rod 7 to the first stage. , The aluminum foil sheet 10 is broken, and the solution starts to move from the chamber 6 to the chamber 11. In this state, the notch 8 provided in the valve stem 7 is connected to the upper and lower sides of the two O-rings 9, so that air flows between the inside and the outside of the chamber 6 and the solution can move smoothly.
[0017]
As described above, since the partition member has the penetrable aluminum foil sheet 10, the pressing needle 13a of the tool needle 13 is simply pushed into the reaction portion 1 side, and the tool needle 13 does not come into contact with the tool. The solution can be poured into the chamber 11 from the chamber 6. In the present embodiment, the corresponding chamber of the reaction part 1 is located immediately below the position of the chamber of the solution storage part 2. However, even if the corresponding chamber is not directly below by forming a flow path or the like. No problem.
[0018]
Next, the inspector once pulls out the tool needle 13 and turns it upside down as shown in FIG. 5 to further push down the valve rod 7 by pushing in the second stage with the long pressing rod 13b. Then, the air is sealed by the upper O-ring 9 and the movement of the solution in the reaction part 1 described later becomes possible. The inspector performs this step for all the chambers 6a to 6m. As described above, since the solution can be poured by pressing the first stage by using the tool needle 13 and the container can be sealed by pressing the second stage, the solution can be poured into the chamber 11 and sealed by the simple operation of pressing. Two tasks can be performed simultaneously.
[0019]
FIG. 6 is a plan sectional view of the reaction part 1. Ten nozzle inlets 4a to 4j are provided on one side surface, and ten nozzle inlets 4k to 4t are also provided on the opposite side surface. The nozzle inlets 4a to 4t communicate with the chambers 11a to 11t through which the solution is stored or where the reaction takes place via the air passages 14a to 14t through which the air flows. However, since the nozzle inlets 4n, 4p, 4q, and 4s are not used in the process of this embodiment, they are not connected to the chamber and are reserved.
[0020]
That is, the nozzle inlets 4a to 4j communicate with the chambers 11a to 11j via the flow paths 14a to 14j, and the opposite nozzle inlets 4k, 41, 4m, 4o, 4r, and 4t flow through the flow paths 14k and 141, respectively. , 14m, 14o, 14r, and 14t, and communicates with the chambers 11k, 11l, 11m, 11o, 11r, and 11t.
[0021]
The sample inlet 3 communicates with the chamber 16, the chambers 11a, 11b, 11c, and 11k communicate with the chamber 16, the chambers 11g and 11o communicate with the chamber 17, and the chambers 11h, 11i, 11j, 11r, and 11t communicate with the chamber 18. ing. Further, the chamber 16 communicates with the chamber 17 via the flow path 19, and the chamber 17 communicates with the chamber 18 via the flow path 20. Chambers 11d, 11e, 11f, 11l, and 11m communicate with the flow path 19 via flow paths 15d, 15e, 15f, 151, and 15m, respectively.
[0022]
A square hole is formed in the bottom surface of the chamber 18, and a DNA microarray 21 made of a glass substrate is attached to the square hole with the probe surface facing upward. In the DNA microarray 21, several hundreds to several hundred thousand kinds of different types of DNA probes are arranged at a high density on a solid surface such as a glass plate of about 1 inch square. By performing a hybridization reaction with a sample DNA using the DNA microarray 21, a large number of genes can be tested at once. Further, these DNA probes are regularly arranged in a matrix, and there is an advantage that the address (how many rows and how many columns) of each DNA probe can be easily taken out as information. Genes to be tested include infectious disease viruses / bacteria, disease-related genes, as well as individual polymorphisms.
[0023]
The first and second hemolytic agents flowing from the chambers 6a and 6b of the solution storage part 2 are accumulated in the chambers 11a and 11b of the reaction part 1, respectively. The magnetic particles flowing from the chamber 6c are accumulated in the chamber 11c, and the first and second extraction cleaning agents flowing from the chambers 6l and 6m are accumulated in the chambers 11l and 11m, respectively. The chamber 11d is filled with an eluate flowing from the chamber 6d, and the chamber 11g is filled with a mixed solution required for PCR (Polymerase Chain Reaction) flowing from the chamber 6g. The cleaning agents flowing from the chambers 6h and 6j are accumulated in the chambers 11h and 11j, respectively, and the alcohol flowing from the chamber 6i is accumulated in the chamber 11i.
[0024]
The chamber 11e is a chamber for storing dust other than blood DNA, the chamber 11f is a chamber for storing waste liquid of the first and second extraction cleaning agents of the chambers 11l and 11m, and the chamber 11r is a chamber for storing the first and second cleaning agents. The chambers 11k, 11o, and 11t are blank chambers provided to prevent the solution from flowing into the nozzle inlet.
[0025]
FIG. 7 shows a processing apparatus for controlling the movement of a solution and various reactions in a biochemical reaction cartridge. The table 22 is a place where a biochemical reaction cartridge is set. On the table 22, an electromagnet 23 that is activated when extracting DNA or the like from the sample in the cartridge, and amplifies DNA from the sample by a method such as PCR. The Peltier element 24 for controlling the temperature during the hybridization between the amplified sample DNA and the DNA probe on the DNA microarray inside the cartridge, and when the unhybridized sample DNA is washed. Peltier elements 25 for controlling the temperature are arranged, and these are connected to a control unit 26 for controlling the entire processing apparatus.
[0026]
Above and below the table 22 in FIG. 7, a pump block provided with electric syringe pumps 27 and 28 and a plurality of pump nozzles 29 and 30 at each of the inlets and outlets for discharging or sucking air by these pumps 27 and 28. 31 and 32 are arranged. Further, the control unit 26 is connected to an input unit 33 for inputting by the examiner.
[0027]
A plurality of well-known electric switching valves (not shown) are arranged between the electric syringe pumps 27 and 28 and the pump nozzles 29 and 30, and are connected to the control unit 26 together with the pumps 27 and 28. The control unit 26 can control to selectively open one of the pump nozzles 29 and 30 out of ten on one side to the pumps 27 and 28 or to close all the pump nozzles 29 and 30. Have been.
[0028]
When the solution is moved from the solution storage section 2 to the reaction section 1 and a processing start signal is inputted, extraction and amplification of DNA and the like are performed inside the reaction section 1 and the amplified sample DNA and the inside of the reaction section 1 are mixed. Hybridization with a DNA probe on a certain DNA microarray and washing of the unlabeled fluorescent-labeled sample DNA and the fluorescent label are performed.
[0029]
In the present embodiment, when blood is used as a sample and an examiner injects blood through a rubber cap of the sample inlet 2 with a syringe, the blood flows into the chamber 16. Thereafter, the examiner places the biochemical reaction cartridge on the table 22 and operates a lever (not shown) to move the pump blocks 31 and 32 in the direction of the arrow in FIG. The pump nozzles 29, 30 are inserted into the nozzle inlet 4 of the reaction part 1.
[0030]
As described in FIG. 6, since the nozzle inlet 4 is concentrated on the two surfaces on both sides of the biochemical reaction cartridge, the pump block including the electric syringe pumps 27 and 28, the electric switching valves, and the pump nozzles 29 and 30 is built in. The shapes and arrangements of 31, 32 are simplified. Furthermore, the pump nozzles 29 and 30 can be inserted only by a simple operation of simultaneously sandwiching the cartridges with the pump blocks 31 and 32 while securing the necessary chambers and flow paths, and the configuration of the pump blocks 31 and 32 is also improved. It's easy. When all the nozzle inlets 4a to 4t are arranged at the same height, that is, linearly, the heights of the flow paths 14a to 14t connected to the nozzle inlets 4a to 4t are all the same, and the production of the flow paths 14a to 14t is completed. It will be easier.
[0031]
In the processing apparatus of FIG. 7, if the pump blocks 31 and 32 are configured to be n times longer for n biochemical reaction cartridges, the n cartridges can be arranged in series to provide n cartridges. The necessary steps can be performed simultaneously, and the biochemical reaction can be performed on a large number of cartridges with a very simple structure.
[0032]
The inspector performs the steps of pouring and sealing the solution described with reference to FIGS. 4 and 5, and then inputs a processing start command through the input unit 33, thereby starting the processing. Of course, a robot arm may be provided on the device side, and the valve rod 7 may be attached to the robot arm to automatically perform the steps described with reference to FIGS. 4 and 5. In this case, the electric syringe is used similarly to the method described later. By operating the pumps 27 and 28, the solution can be smoothly poured. Furthermore, instead of performing the pouring step immediately before using the biochemical reaction cartridge as in the present embodiment, it is also possible to pour individual solutions immediately before the solutions are needed.
[0033]
FIG. 8 is a flowchart illustrating a processing procedure in the processing apparatus according to the present embodiment. First, in step S1, the control unit 26 opens only the nozzle inlets 4a and 4k, discharges air from the electric syringe pump 27, sucks air from the pump 28, and puts the first hemolytic agent in the chamber 11a into blood. Pour into chamber 16. At this time, depending on the viscosity of the hemolytic agent and the resistance of the flow path, if the suction of the air from the pump 28 is controlled so as to start 10 to 200 msec after the start of the discharge of the air from the pump 27, at the head of the flowing solution The solution flows smoothly without jumping out.
[0034]
As described above, by controlling the pressurization and decompression by shifting the timing of the supply and suction of the air, the solution can flow smoothly, but the suction of the air from the electric syringe pump 28 If fine control is performed, such as increasing the pressure linearly from the start of the discharge, the fluid can flow more smoothly. Further, by using both pressurization and decompression, the pressure generated in the reaction part 1 can be reduced. As a result, when there is a branch channel or a chamber in which the solution does not want to flow during the movement of the solution, It also has the effect of preventing it from flowing into it. The same applies to the transfer of the following solution.
[0035]
The control of the supply of air can be easily realized by using the electric syringe pumps 27 and 28. Only the nozzle inlets 4a and 4o are opened, and the discharge and suction of air are alternately repeated by the syringe pumps 27 and 28. The above operation of flowing the solution through the flow path 19 and returning the solution to the flow path 19 is repeated to perform stirring. Alternatively, the air may be continuously discharged from the pump 28 and stirred while generating bubbles.
[0036]
FIG. 9 is a cross-sectional view of the reaction part 1 passing through the chambers 11a, 16 and 11k of FIG. 6, in which the pump nozzle 29 is inserted into the nozzle inlet 4a and pressurized, and the pump nozzle 30 is inserted into the nozzle inlet 4k. This shows a state where the pressure is reduced and the first hemolytic agent in the chamber 11a flows into the chamber 16 containing blood. The chamber 11a actually communicates with the solution storage part 2, but for convenience, a ceiling is provided so that the chamber 11a does not communicate therewith.
[0037]
In FIG. 8, in the next step S2, only the nozzle inlets 4b, 4k are opened, and similarly, the second hemolytic agent in the chamber 11b is poured into the chamber 16, and in step S3, only the nozzle inlets 4c, 4k are opened. Then, the magnetic particles in the chamber 11c are poured into the chamber 16. In both steps S2 and S3, stirring is performed in the same manner as in step S1. In step S3, the DNA obtained by lysing the cells in steps S1 and S2 is attached to the magnetic particles.
[0038]
Then, in step S4, the electromagnet 23 is turned on, only the nozzle inlets 4e, 4k are opened, air is discharged from the electric syringe pump 28, air is sucked from the pump 27, and the solution in the chamber 16 is moved to the chamber 11e. During this movement, the magnetic particles and the DNA are captured on the electromagnet 23 in the flow path 19. The suction and discharge of the electric syringe pumps 27 and 28 are alternately repeated, and the solution is reciprocated twice between the chambers 16 and 11e to increase the DNA capturing efficiency. Increasing the number further increases the capture efficiency, but adds extra processing time.
[0039]
As described above, the magnetic particles are used to capture DNA on a small flow path having a width of about 1 to 2 mm and a height of about 0.2 to 1 mm, and in a flowing state. Can be. The same applies when the capture target substance is RNA or protein.
[0040]
Next, in step S5, the electromagnet 23 is turned off, only the nozzle inlets 4f, 4l are opened, air is discharged from the electric syringe pump 28, air is sucked from the pump 27, and the first extraction washing liquid in the chamber 11l is supplied to the chamber 11l. Move to 11f. At this time, the magnetic particles and the DNA captured in step S4 move together with the extraction washing solution to perform washing. After two reciprocations in the same manner as in step S4, the electromagnet 23 is turned on, and two reciprocations are performed in the same manner to collect the magnetic particles and DNA on the electromagnet 23 in the flow path 19, and the solution is returned to the chamber 11l. deep.
[0041]
In step S6, the second extraction cleaning liquid in the chamber 11m is further cleaned by performing the same process as step S5 using the nozzle inlets 4f and 4m. In step S7, while the electromagnet 23 is kept on, only the nozzle inlets 4d and 4o are opened, the air is discharged from the electric syringe pump 27, the air is sucked from the pump 28, and the eluate in the chamber 11d is moved to the chamber 17. I do.
[0042]
At this time, the magnetic particles and the DNA are separated by the action of the eluate, only the DNA moves to the chamber 17 together with the eluate, and the magnetic particles remain in the flow path 19, thus extracting and purifying the DNA. Is By preparing the chambers 11 l and 11 m containing the extraction washing liquid and the chamber 11 f containing the waste liquid after washing, it becomes possible to perform extraction and purification of DNA in the biochemical reaction cartridge 1.
[0043]
Next, in step S8, only the nozzle inlets 4g and 4o are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the PCR reagent in the chamber 11g flows into the chamber 17. Further, only the nozzle inlets 4g and 4t are opened, and the discharge and suction of air are alternately repeated by the electric syringe pumps 27 and 28, and the solution in the chamber 16 is caused to flow through the flow path 20, and then the operation of returning the solution is repeated. Do. After controlling the Peltier element 24 to maintain the solution in the chamber 17 at a temperature of 96 ° C. for 10 minutes, the steps of 96 ° C. for 10 seconds, 55 ° C. for 10 seconds, and 72 ° C. for 1 minute are repeated 30 times. Then, the eluted DNA is amplified by performing PCR.
[0044]
In step S9, only the nozzle inlets 4g and 4t are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the solution in the chamber 17 is moved to the chamber 18. Further, the Peltier device 25 is controlled to carry out hybridization while keeping the solution in the chamber 18 at 45 ° C. for 2 hours. At this time, the discharge and suction of the air are alternately repeated by the pumps 27 and 28, the solution in the chamber 18 is moved to the flow path 15t, and the operation of returning the solution after that is repeated, and the hybridization is advanced while stirring.
[0045]
Next, in step S10, while maintaining the same 45 ° C., only the nozzle inlets 4h and 4r are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the solution in the chamber 18 is discharged. Is moved to the chamber 11r, and the first cleaning liquid in the chamber 11h flows into the chamber 11r through the chamber 18. The suction and discharge of the pumps 27 and 28 are alternately repeated, and the solution is reciprocated twice between the chambers 11h, 18 and 11r, and finally returned to the chamber 11h. In this manner, the fluorescently labeled sample DNA and the fluorescent label that have not been hybridized are washed.
[0046]
FIG. 10 is a cross-sectional view passing through the chamber 11h, the chamber 18, and the chamber 11r in FIG. 6, in which the pump nozzle 29 is inserted into the nozzle inlet 4h to be pressurized, and the pump nozzle 30 is inserted into the nozzle inlet 4r to be depressurized. The state where the first cleaning liquid in the chamber 11h flows into the chamber 11r through the chamber 18 is shown. The chamber 11h is actually in communication with the solution storage part 2, but for convenience, a ceiling is provided so that the chamber 11h is not in communication.
[0047]
In FIG. 8, in step S11, while maintaining the same 45 ° C., the second cleaning liquid in the chamber 11j is further cleaned by performing the same process as in step S10 using the nozzle inlets 4j and 4r. Return to Since the chambers 11h and 11j containing the washing liquid and the chamber 11r containing the waste liquid after washing are prepared, the DNA microarray 21 can be washed in the biochemical reaction cartridge.
[0048]
In step S12, only the nozzle inlets 4i and 4r are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the alcohol in the chamber 11i is moved to the chamber 11r through the chamber 18. Thereafter, only the nozzle inlets 4i and 4t are opened, air is discharged from the pump 27, and air is sucked from the pump 28 to dry the inside of the chamber 18.
[0049]
Thereafter, when the examiner operates a lever (not shown), the pump blocks 31 and 32 are moved away from the biochemical reaction cartridge by a mechanism not shown, and the pump nozzles 29 and 30 are disengaged from the nozzle inlet 4 of the cartridge. Then, the inspector inserts the cartridge into a DNA microarray reader such as a well-known scanner (not shown) to perform measurement and analysis.
[0050]
Some of the embodiments of the present invention are listed below.
[0051]
[Embodiment 1] A reaction part including a chamber and a flow path, and a solution storage part that is separated from or separate from the reaction part and stores a solution at a position corresponding to the chamber, and a solution from the solution storage part A biochemical reaction cartridge provided with a partition member that can penetrate between the solution storage part and the reaction part in order to move the liquid to the chamber of the reaction part.
[0052]
[Embodiment 2] The biochemical reaction cartridge according to Embodiment 1, wherein the partition member can be penetrated by being pushed by a valve stem.
[0053]
[Embodiment 3] By the first stage pushing of the valve stem by the tool needle to the partition member, the chamber is opened to the outside, and the solution in the solution storage part moves to the chamber, 3. The biochemical reaction cartridge according to Embodiment 2, wherein the chamber is hermetically sealed from the outside by pushing in the second stage.
[0054]
[Embodiment 4] The partition member includes two pressing rods having different lengths, wherein the first-stage pressing uses a short pressing bar, and the second-stage pressing uses a long pressing bar. The biochemical reaction cartridge according to aspect 3.
[0055]
Fifth Embodiment The biochemical reaction cartridge according to the fourth embodiment, wherein the two pressing rods having different lengths are arranged on the same axis in opposite directions.
[0056]
【The invention's effect】
As described above, the biochemical reaction cartridge according to the present invention includes a reaction part including a chamber and a flow path, and a solution storage part that is separated from the reaction part or separate from the reaction part and stores a solution such as a reagent or a cleaning agent. Having a member that can penetrate a member that separates the solution from the solution storage part to the reaction part, or a member that allows the solution storage part and the reaction part to come into contact and penetrate their walls, The solution can be prepared in the biochemical reaction cartridge at any time, reducing the need for replenishment of reagents and mistakes in the types of reagents. There is an advantage that an intended reaction can be correctly caused without flowing into another chamber.
[Brief description of the drawings]
FIG. 1 is a perspective view of a biochemical reaction cartridge according to an embodiment.
FIG. 2 is a plan sectional view of a solution storage part.
FIG. 3 is a partial cross-sectional view of the biochemical reaction cartridge during storage.
FIG. 4 is a cross-sectional view of a part of the biochemical reaction cartridge in a state where a valve stem is pushed in one step.
FIG. 5 is a partial cross-sectional view of the biochemical reaction cartridge in a state where the valve stem is pushed in two steps.
FIG. 6 is a plan sectional view of a reaction part.
FIG. 7 is a block diagram of a processing apparatus for controlling movement of a solution and various reactions in a biochemical reaction cartridge according to the present embodiment.
FIG. 8 is a flowchart of a processing procedure.
FIG. 9 is a sectional view of a part of the chamber in FIG. 6;
FIG. 10 is a sectional view of a part of the chamber of FIG. 6;
[Explanation of symbols]
1 Reaction part
2 Solution storage part
3 Sample entrance
4 Nozzle entrance
5, 10 Aluminum foil sheet
7 Valve stem
8 Notch
9 O-ring
11, 16-18 chambers
13 Needle for tool
15, 19, 20 channels
21 DNA microarray
22 tables
26 control unit
27, 28 Electric syringe pump
29, 30 Pump nozzle
31, 32 Pump block

Claims (1)

A reaction part including a chamber and a flow path; and a solution storage part which is separated from or separate from the reaction part and stores a solution at a position corresponding to the chamber. A biochemical reaction cartridge provided with a partition member that can penetrate between the solution storage part and the reaction part in order to move the cartridge.

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