JP2005345177A - Biochemical reaction cartridge - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To store, in a cartridge, analytical information of a transition, a procedure and a result in a cartridge process for conducting a series of biochemical reactions without taking out a solution after injection of a specimen by a user, by moving the solution with an external pump without building a pump in. <P>SOLUTION: An IC chip is provided in the cartridge to allow nonvolatile storage of the information, and transceiving of the information with an external processor. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a biochemical reaction cartridge that is used by being incorporated in an apparatus for analyzing cells, microorganisms, chromosomes, nucleic acids, and the like in a specimen using biochemical reactions such as antigen-antibody reaction and nucleic acid hybridization reaction.

  Many analyzers for analyzing a sample such as blood use an immunological method utilizing an antigen-antibody reaction or a method utilizing nucleic acid hybridization. For example, antibodies such as antibodies or antigens that specifically bind to the detected substance or antigens or single-stranded nucleic acids are used as probes, and immobilized on a solid surface such as fine particles, beads, glass plates, etc., and the detected substance and antigen-antibody reaction Alternatively, nucleic acid hybridization is performed. And using a labeling substance having a specific interaction carrying a labeling substance having high detection sensitivity such as an enzyme, a fluorescent substance, a luminescent substance, such as a labeled antibody, a labeled antigen or a labeled nucleic acid, An antigen-antibody compound or a double-stranded nucleic acid is detected to detect the presence or absence of a test substance or to quantify the test substance.

  For example, US Pat. No. 5,445,934 discloses a so-called DNA array in which a large number of DNA (deoxyribonucleic acid) probes having different base sequences are arranged in an array on a substrate. Is disclosed.

  Anal. Biochem., 270 (1), 103-111, 1999 discloses a method for preparing a protein array having a structure like a DNA array by arranging many kinds of proteins on a membrane filter. As described above, it has become possible to test a very large number of items at once by using a DNA array, a protein array, or the like.

  We also propose disposable biochemical reaction cartridges that carry out necessary reactions internally for the purpose of reducing sample contamination, improving reaction efficiency, miniaturizing equipment, and simplifying work in various sample analysis methods. Has been. For example, in Japanese Patent Application Laid-Open No. 11-509094, a plurality of chambers are arranged in a biochemical reaction cartridge including a DNA array, a solution is moved by differential pressure, and DNA in a sample is extracted or amplified inside the cartridge. Alternatively, a biochemical reaction cartridge that enables a reaction such as hybridization is disclosed.

  As a method for injecting a solution from the outside into the biochemical reaction cartridge, there is a method using an external syringe pump or a vacuum pump. As a method for moving a solution inside a biochemical reaction cartridge, a method using gravity, capillary action, or electrophoresis is known. As a micro pump that is small and can be disposed inside the biochemical reaction cartridge, Japanese Patent No. 2832117 uses a heating element, Japanese Patent Application Laid-Open No. 2000-274375 uses a piezoelectric element, Table No. 11-509094 discloses a diaphragm pump.

On the other hand, an information storage IC is provided on a DNA chip used for biochemical reactions to provide DNA.
The chip is being identified. Japanese Patent Application Laid-Open No. 2001-147231 discloses DNA
It is disclosed that the base sequence information of the chip and the information of the object to be inspected are written in the information storage IC.

  Thus, it is preferable to use a disposable cartridge containing a necessary solution from the viewpoint of prevention of secondary infection and contamination and usability, but there is a problem that a cartridge containing a pump is expensive. Disposable with a structure that allows a series of biochemical reactions to proceed without causing the solution to flow outside after the user injects the sample by moving the solution by the action of an external pump A biochemical reaction cartridge is utilized.

  A DNA chip only handles a single biochemical reaction called hybridization, but such a cartridge continuously performs various biochemical reactions, and therefore requires comprehensive management. The operation of the cartridge is recorded on the device side that drives the cartridge. It is desired to store the information more safely. Furthermore, it is desired to cope with the variety of cartridge types.

  In order to achieve the above object, the biochemical reaction cartridge according to the present invention includes a specimen inlet, a chamber for containing a specimen, a chamber for containing a reagent, a chamber for flowing a mixed liquid / reaction liquid of the specimen / reagent, A flow path and a plurality of nozzle inlets for inserting nozzles for pressurization or decompression, the nozzle inlets communicating with the chamber, and air is interposed between the nozzle inlet and the chamber, The air is pressurized or depressurized by a nozzle to move the specimen / reagent, and a series of biochemical reactions are performed inside, and it has a storage means for storing the transition of the process.

  Further, the storage means is a means for storing information for analysis when inspecting a process procedure or a biochemical reaction result.

  Since the biochemical reaction cartridge according to the present invention keeps the transition of the biochemical reaction as information in the built-in storage means, when a human error occurs in the data processing on the processing device side later, as a data backup function There is an effect that it can be used for matching important information. In addition, since the final processing time is also stored, in the next process, the period after the process can be calculated at the start of the process and can be added as a process parameter, so that more accurate processing can be performed.

  Furthermore, there is an effect that it is possible to process a cartridge of a type that is not registered in the processing apparatus. Also, since the analysis information is in the cartridge when the biochemical reaction result is inspected, there is an effect that it is possible to analyze a cartridge of a type that is not registered in the apparatus to be inspected.

  The present invention will be described in detail based on the illustrated embodiment.

  FIG. 1 shows an external view of a biochemical reaction cartridge 1 according to the present embodiment. A specimen inlet 2 for injecting a specimen such as blood using a syringe or the like is provided at the top of the cartridge 1 and is provided with a rubber cap. It is sealed. Further, a plurality of nozzle inlets 3 are provided on the side surface of the cartridge 1 to pressurize or depressurize by inserting nozzles to move the solution inside, and a rubber cap is fixed to each nozzle inlet 3, The surface has the same configuration.

  The main body of the biochemical reaction cartridge 1 is made of a transparent or translucent synthetic resin such as polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene (ABS) copolymer, polystyrene, polycarbonate, polyester, polyvinyl chloride or the like. ing. Note that the material of the main body may not be transparent when the reactant in the cartridge 1 does not require optical measurement.

  An IC chip 25 is provided inside the cartridge 1. The IC chip 25 includes a non-volatile storage unit and a communication unit for receiving power from outside and transmitting / receiving a signal.

  In the storage unit of the IC chip 25, the cartridge ID is written.

  There are also cases where information for analysis of cartridge type, processing step procedure, and biochemical reaction result is written.

  Usually, the processing apparatus stores a processing step procedure for each type of cartridge. Then, the processing device determines the type of cartridge written in the IC chip 25, selects an appropriate processing step procedure, and performs processing according to the procedure.

  If the cartridge type is a type that is not stored in the processing device, the processing device calls the processing procedure written in the IC chip 25 and performs processing in accordance with the procedure.

  In the processing step procedure, a temperature condition or the like applied to cause a biochemical reaction is also defined depending on the processing step.

  FIG. 2 shows a plan cross-sectional view of the biochemical reaction cartridge 1. Ten nozzle inlets 3a to 3j are provided on one side surface, and ten nozzle inlets 3k to 3t are provided on the opposite side surface. It has been. Each nozzle inlet 3a-3t is connected to the chamber 5 which is a place which stores a solution or a reaction takes place via the air flow paths 4a-4t through which each air flows.

  However, since the nozzle inlets 3n, 3p, 3q, and 3s are not used in the process of the present embodiment, they are not communicated with the chamber 5 and are reserved. That is, the nozzle inlets 3a to 3j are communicated with the chambers 5a to 5j via the flow paths 4a to 4j, and the opposite nozzle inlets 3k, 3l, 3m, 3o, 3r, and 3t are respectively connected to the flow paths 4k, 4l, The chambers 5k, 5l, 5m, 5o, 5r, and 5t are communicated with each other through 4m, 4o, 4r, and 4t.

  The specimen inlet 2 communicates with the chamber 7, the chambers 5a, 5b, 5c, and 5k communicate with the chamber 7, the chambers 5g and 5o communicate with the chamber 8, and the chambers 5h, 5i, 5j, 5r, and 5t communicate with the chamber 9, respectively. ing. Further, the chamber 7 communicates with the chamber 8 via the flow path 10, and the chamber 8 communicates with the chamber 9 via the flow path 11. Chambers 5d, 5e, 5f, 5l, and 5m are communicated with the flow path 10 through flow paths 6d, 6e, 6f, 6l, and 6m, respectively.

  In addition, a square hole is formed in the bottom surface of the chamber 9, and several tens to several hundreds of thousands of different types of DNA probes are formed in the square hole on a solid surface such as a glass plate having a size of about a square inch. The DNA microarrays 12 arranged in the above are attached with the probe surface facing up.

  By performing a hybridization reaction with the sample DNA using this DNA microarray 12, a large number of genes can be examined at once. In addition, these DNA probes are regularly arranged in a matrix, and the address (how many rows and how many columns) of each DNA probe can be easily taken out as information. In addition to infectious disease viruses, bacteria, and disease-related genes, genes to be tested include individual polymorphisms.

  In the chambers 5a and 5b, a first hemolytic agent containing EDTA that destroys cell walls and a second hemolytic agent containing a protein denaturant such as a surfactant are accumulated. Silica-coated magnetic particles to which DNA is adsorbed are accumulated in the chamber 5c, and the first and second extraction detergents used for purifying the DNA at the time of DNA extraction in the chambers 5l and 5m. Is accumulated.

  The chamber 5d contains an eluate composed of a low-concentration salt buffer that elutes DNA from magnetic particles, and the chamber 5g contains primers necessary for PCR, polymerase, dNTP solution, buffer, Cy-3dUTP containing a fluorescent agent, and the like. The mixture is filled. The chambers 5h and 5j have a cleaning agent containing a detergent for washing the fluorescently labeled sample DNA and fluorescent label, and the chamber 5i has the inside of the chamber 9 including the DNA microarray 12 dried. Alcohol for accumulating is accumulated.

  The chamber 5e is a chamber for collecting dust other than blood DNA, the chamber 5f is a chamber for collecting waste liquids of the first and second extraction detergents in the chambers 5l and 5m, and the chamber 5r is for the first and second detergents. Chambers 5k, 5o, and 5t are blank chambers provided to prevent the solution from flowing into the nozzle inlet.

  When a liquid sample such as blood is injected into the biochemical reaction cartridge 1 and set in a processing apparatus to be described later, DNA and the like are extracted and amplified inside the cartridge 1, and the amplified sample DNA and the cartridge 1 are further extracted. Hybridization is performed with a DNA probe on a DNA microarray in the inside, and the fluorescence-labeled sample DNA that has not been hybridized and the fluorescent label are washed.

  FIG. 3 shows a schematic diagram of a processing apparatus for controlling the movement of the solution in the biochemical reaction cartridge 1 and various reactions. The table 13 shows the position where the cartridge 1 is set. On this table 13, an electromagnet 14 that is operated when DNA or the like from the sample is extracted in the cartridge 1, and DNA (sample) from the sample are subjected to PCR (Polymerase Chain Reaction). Peltier element 15 for controlling the temperature when amplifying by a method such as), when the amplified sample DNA is hybridized with the DNA probe on the DNA microarray in the cartridge 1, and the sample that has not been hybridized A Peltier element 16 for controlling the temperature when DNA is washed, and a communication unit 26 for transmitting power to the IC chip 25 and transmitting / receiving signals to the IC chip 25 in the cartridge 1 to store data from the processing device. Are connected to a control unit 17 that controls the entire processing apparatus. ing.

  On both sides of the table 13, there are electric syringe pumps 18, 19 and pump blocks in which ten pump nozzles 20, 21 are provided on each side, with an inlet / outlet for discharging or sucking air by these pumps 18, 19. 22 and 23 are arranged. A plurality of electric switching valves (not shown) are arranged between the electric syringe pumps 18 and 19 and the pump nozzles 20 and 21, and are connected to the controller 17 together with the pumps 18 and 19. Moreover, the control part 17 is connected to the input part 24 which an inspector inputs. The control unit 17 performs control to selectively open the pump nozzles 20 and 21 one by one with respect to the electric syringe pumps 18 and 19 or to close all the pump nozzles among the ten nozzles on one side. ing.

  In this embodiment, blood flows into the chamber 7 when blood is sampled and the examiner injects blood through the rubber cap of the sample inlet 2 with a syringe. Thereafter, the examiner places the biochemical reaction cartridge 1 on the table 13 and operates the lever (not shown) to move the pump blocks 22 and 23 in the direction of the arrow in FIG. A rubber cap is inserted through the nozzle inlets 3 on both sides of the cartridge 1.

  In addition, since the nozzle inlets 3a to 3t are concentrated on two surfaces, that is, both sides of the biochemical reaction cartridge 1, the shapes of the electric syringe pumps 18, 19, the electric switching valve, the pump blocks 22, 23 having built-in pump nozzles, etc. , Can simplify the arrangement. Furthermore, the pump nozzles 20 and 21 can be inserted by a simple operation of simultaneously sandwiching the cartridge 1 by the pump blocks 22 and 23 while securing the necessary chambers 5 and flow paths. The configuration can be simplified. Then, by arranging the nozzle inlets 3a to 3t all at the same height, that is, linearly, the heights of the flow paths 4a to 4t connected to the nozzle inlets 3a to 3t are all the same, and the flow paths 4a to 4t. Is easy to manufacture.

  In the processing apparatus of FIG. 3, if the pump blocks 22 and 23 are made to be n times longer for n biochemical reaction cartridges 1, n cartridges 1 are arranged in series, so that n Necessary processes can be simultaneously performed on the cartridge 1, and a biochemical reaction can be performed on a large number of cartridges with a very simple configuration.

  The process starts when the inspector inputs a process start command at the input unit 24. FIG. 4 is a flowchart illustrating a processing procedure in the processing apparatus according to the present embodiment. First, in step S1, the control unit 17 opens only the nozzle inlets 3a and 3k, discharges air from the electric syringe pump 18, sucks air from the pump 19, and puts the first hemolytic agent in the chamber 5a into the blood. Pour into the chamber 7. At this time, depending on the viscosity of the hemolytic agent and the resistance of the flow path, if the suction of the air from the pump 19 is controlled to start 10 to 200 milliseconds after the start of the discharge of the air from the pump 18, The solution does not jump out at the beginning of the flowing solution, and the solution flows smoothly.

  In this way, by controlling the pressurization and decompression by shifting the timing of air supply and suction, the solution can flow smoothly, but the suction of air by the electric syringe pump 19 If fine control such as linear increase from the start is performed, the solution can flow more smoothly. The same applies to the movement of the following solutions.

  The control of the air supply can be easily realized by using the electric syringe pumps 18 and 19. Only the nozzle inlets 3 a and 3 o are opened, and the discharge and suction of the air are alternately repeated by the pumps 18 and 19. The operation of flowing the solution through the flow path 10 and then returning to the flow path 10 is repeated to perform stirring. Alternatively, the air may be continuously discharged from the pump 19 and stirred while generating bubbles.

  FIG. 5 is a cross-sectional view through the chambers 5a, 7 and 5k shown in FIG. 2. The pump nozzle 20 is inserted into the nozzle inlet 3a for pressurization, and the pump nozzle 21 is inserted into the nozzle inlet 3k for depressurization. This shows that the first hemolytic agent flows into the chamber 7 containing blood.

  At the end of the first hemolytic agent agitation process, the processing device causes the first hemolytic agent to flow through the communication unit 26, and the IC chip 25 stores the completion of the agitation process, various conditions, and the end time. .

  In FIG. 4, next, in step S2, only the nozzle inlets 3b and 3k are opened, and the second hemolytic agent in the chamber 5b is poured into the chamber 7 in the same manner. In step S3, only the nozzle inlets 3c and 3k are opened, and the same Then, the magnetic particles in the chamber 5 c are poured into the chamber 7. In steps S2 and S3, stirring is performed in the same manner as in step S1. In step S3, the DNA obtained by dissolving the cells in steps S1 and S2 adheres to the magnetic particles.

  At the end of the second hemolytic agent agitation process, the processing device causes the IC chip 25 to store the second hemolytic agent flowing in via the communication unit 26, the completion of the agitation process, various conditions, and the end time. At the end of the magnetic particle stirring step, the magnetic particles are poured into the IC chip 25 via the communication unit 26, and the completion of the stirring step, various conditions, and the end time are stored in the IC chip 25.

  In step S4, the electromagnet 14 is turned on, only the nozzle inlets 3e and 3k are opened, the air is discharged from the electric syringe pump 19, the air is sucked from the pump 18, and the solution in the chamber 7 is moved to the chamber 5e. During this movement, the magnetic particles and DNA are captured on the electromagnet 14 in the flow path 10. The suction and discharge of the pumps 18 and 19 are alternately repeated, and the solution is reciprocated twice between the chambers 7 and 5e, thereby improving the DNA capture efficiency. Furthermore, if the number of times is increased, the capture efficiency can be further increased, but extra processing time is also required.

  In this way, DNA is captured in a flowing state on a small channel having a width of about 1 to 2 mm and a height of about 0.2 to 1 mm using magnetic particles, so that it can be captured extremely efficiently. Can do. The same applies when the capture target substance is RNA or protein.

  The processing device stores the completion of the magnetic particle and DNA supplementing step, various conditions, and the end time in the IC chip 25 via the communication unit 26 at the end of the magnetic particle and DNA supplementing step.

  Next, in step S5, the electromagnet 14 is turned off, only the nozzle inlets 3f and 3l are opened, the air is discharged from the electric syringe pump 19, the air is sucked from the pump 18, and the first extraction cleaning liquid in the chamber 5l is removed. Move to chamber 5f. At this time, the magnetic particles and DNA captured in step S4 are moved together with the extraction washing liquid to perform washing. After reciprocating twice in the same manner as in step S4, the electromagnet 14 is turned on, and reciprocating twice in the same manner to collect the magnetic particles and DNA on the electromagnet 14 in the flow path 10 and return the solution to the chamber 5l. .

  The processing apparatus captures the magnetic particles and DNA after washing with the first extraction washing liquid via the communication unit 26 after the washing with the first extraction washing liquid and at the end of the supplement process of the magnetic particles and DNA. The completion of the process, various conditions, and the end time are stored in the IC chip 25.

  In step S6, using the nozzle inlets 3f and 3m, the second extraction cleaning liquid in the chamber 5m is further cleaned by performing the same process as in step S5.

  The processing apparatus captures the magnetic particles and DNA after washing with the second extraction washing liquid via the communication unit 26 after the washing with the second extraction washing liquid and at the end of the supplement process of the magnetic particles and DNA. The completion of the process, various conditions, and the end time are stored in the IC chip 25.

  In step S7, with the electromagnet 14 turned on, only the nozzle inlets 3d and 3o are opened, air is discharged from the electric syringe pump 18, and air is sucked from the pump 19, whereby the eluate from the chamber 5d is supplied to the chamber 8. Moving.

  At this time, the magnetic particles and DNA are separated by the action of the eluate, and only the DNA moves to the chamber 8 together with the eluate, and the magnetic particles remain in the flow path 10.

  The treatment apparatus flows the eluate, and at the end of the separation process of magnetic particles and DNA, flows the eluate via the communication unit 26, completes the separation process of magnetic particles and DNA, and various conditions and ends. The time is stored in the IC chip 25.

  In this way, DNA extraction and purification are performed. Since the chamber containing the extracted washing liquid and the chamber containing the waste liquid after washing are prepared, DNA can be extracted and purified in the biochemical reaction cartridge 1.

  Next, in step S8, only the nozzle inlets 3g and 3o are opened, air is discharged from the electric syringe pump 18, the air is sucked from the pump 19, and the PCR drug in the chamber 5g is poured into the chamber 8. Further, only the nozzle inlets 3g and 3t are opened, and the pumps 18 and 19 alternately repeat the discharge and suction of air to flow the solution in the chamber 8 through the flow path 11, and then repeat the operation of returning to the subsequent stirring. Then, after controlling the Peltier element 15 to keep the solution in the chamber 8 at a temperature of 96 ° C. for 10 minutes, the process of 96 ° C. · 10 seconds, 55 ° C. · 10 seconds, 72 ° C. · 1 minute is repeated 30 times. The amplified DNA is amplified by PCR.

  At the end of the PCR amplification process of the eluted DNA, the processing apparatus stores in the IC chip 25 the completion of the PCR amplification process of the eluted DNA, various conditions, and the end time via the communication unit 26.

  In step S9, only the nozzle inlets 3g and 3t are opened, air is discharged from the electric syringe pump 18, the air is sucked from the pump 19, and the solution in the chamber 8 is moved to the chamber 9. Further, the Peltier element 16 is controlled to perform hybridization by holding the solution in the chamber 9 at 45 ° C. for 2 hours. At this time, the pumps 18 and 19 alternately repeat the discharge and suction of air, move the solution in the chamber 9 to the flow path 6t, and repeat the operation of returning to the subsequent, and the hybridization proceeds.

  At the end of the hybridization process, the processing apparatus stores the completion of the hybridization process, various conditions, and the end time in the IC chip 25 via the communication unit 26.

  Next, in step S10, while maintaining the same 45 ° C., this time, only the nozzle inlets 3h and 3r are opened, air is discharged from the electric syringe pump 18, air is sucked from the pump 19, and the solution in the chamber 9 is discharged. The first cleaning liquid in the chamber 5h is poured into the chamber 5r through the chamber 9 while moving to the chamber 5r. The suction and discharge of the pumps 18 and 19 are alternately repeated to reciprocate the solution twice between the chambers 5h, 9, and 5r, and finally return to the chamber 5h. In this way, the fluorescently labeled sample DNA and the fluorescent label that have not been hybridized are washed.

  FIG. 6 is a cross-sectional view through the chambers 5h, 9, and 5r of FIG. 2, the pump nozzle 20 is inserted into the nozzle inlet 3h and pressurized, the pump nozzle 21 is inserted into the nozzle inlet 3r, and the pressure is reduced. The first cleaning liquid flows through the chamber 9 into the chamber 5r.

  At the end of the cleaning process with the first cleaning liquid, the processing apparatus stores in the IC chip 25 the completion of the cleaning process with the first cleaning liquid, various conditions, and the end time via the communication unit 26.

  In FIG. 4, while maintaining the same 45 ° C. in step S11, the second cleaning liquid in the chamber 5j is further cleaned through the same process as in step S10 using the nozzle inlets 3j and 3r, and finally the chamber 5j is placed in the chamber 5j. return. Thus, since the chambers 5h and 5j containing the cleaning liquid and the chamber 5r containing the waste liquid after the cleaning are prepared, the DNA microarray 12 can be cleaned in the biochemical reaction cartridge 1.

  At the end of the cleaning process with the second cleaning liquid, the processing apparatus stores in the IC chip 25 the completion of the cleaning process with the second cleaning liquid, various conditions, and the end time via the communication unit 26.

  In step S12, only the nozzle inlets 3i and 3r are opened, air is discharged from the electric syringe pump 18, the air is sucked from the pump 19, and the alcohol in the chamber 5i is moved through the chamber 9 to the chamber 5r. Thereafter, only the nozzle inlets 3i and 3t are opened, air is discharged from the pump 18, and air is sucked from the pump 19 to dry the inside of the chamber 9.

  At the end of the movement and drying process of the alcohol, the processing device stores in the IC chip 25 the completion of the movement and drying process of the alcohol, various conditions, and the end time via the communication unit 26.

  When the examiner operates a lever (not shown), the pump blocks 22 and 23 move away from the biochemical reaction cartridge 1, and the pump nozzles 20 and 21 are detached from the nozzle inlet 3 of the cartridge 1.

  In this way, the transition of each process is stored in the IC chip 25 built in the cartridge 1.

  The inspector inserts the cartridge 1 into a well-known DNA microarray reading device such as a scanner to perform measurement and analysis. Such a reading device can be incorporated into a processing device and operated continuously.

  If the cartridge type is a type that is not stored in the reading device, the reading device calls the analysis information written in the IC chip 25 after the measurement and performs analysis. .

  In the DNA chip used in the biochemical reaction cartridge 1 for detecting infectious diseases, several infectious diseases are detected at a time by selecting probes constituting the array.

  Here, the detection principle will be schematically described.

  For example, in a 5 × 5 matrix-arranged probe array, five patterns as shown in FIGS. 7 to 11 are designed when infectious diseases A to E are detected alone. ● indicates that the target is hybridized with the probe and is fluorescent with a fluorescent label, and ○ indicates that the target is not hybridized with the probe and is not fluorescent with a fluorescent label.

  We are analyzing whether or not we have an infection by looking at how the actual detection pattern matches these patterns. The pattern used for such analysis is stored in the storage means of the biochemical reaction cartridge 1 in accordance with the configuration of the array.

  Some of the embodiments of the present invention are listed below.

  [Embodiment 1] Specimen inlet, chamber for containing specimen, chamber for containing reagent, chamber for flowing mixed liquid / reaction liquid of specimen / reagent, flow path, and nozzle for pressurizing or depressurizing A plurality of nozzle inlets through which the nozzle inlet communicates with the chamber, air is interposed between the nozzle inlet and the chamber, and the air is pressurized or depressurized by the nozzle. A biochemical reaction cartridge having a storage means for storing a transition of a process, in which a specimen / reagent moves and a series of biochemical reactions are performed inside.

  [Embodiment 2] The biochemical reaction cartridge according to Embodiment 1 in which the nozzle inlets are concentrated on two surfaces.

  [Embodiment 3] The biochemical reaction cartridge according to Embodiment 2 in which the nozzle inlets are arranged in a straight line.

  [Embodiment 4] The biochemical reaction cartridge according to Embodiment 2, wherein the two surfaces are side surfaces on both sides.

  [Embodiment 5] A magnetic particle adsorbed by a target substance of DNA, RNA, or protein is used as one of the reagents, and after the adsorption reaction, the magnetic force of a magnet arranged near the flow path is applied to the middle of the movement. The biochemical reaction cartridge according to Embodiment 1, wherein the magnetic substance particles are captured on the target material to purify the target material.

  [Embodiment 6] The biochemical reaction cartridge according to Embodiment 1 having a chamber containing a cleaning liquid and a chamber containing a waste liquid after cleaning.

It is a perspective view of the biochemical reaction cartridge of an embodiment. It is a plane sectional view of a biochemical reaction cartridge. It is a block block diagram of the processing apparatus which controls the movement of a solution in a biochemical reaction cartridge, and various reaction. It is a flowchart figure of a processing procedure. It is a longitudinal cross-sectional view of some chambers. It is a longitudinal cross-sectional view of another one part chamber. It is a pattern detected in the case of infection A. This is a pattern detected in the case of infection B. This is a pattern detected in the case of infection C. It is a pattern detected in the case of infection D. It is a pattern detected in the case of infection E.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Biochemical reaction cartridge 2 Specimen inlet 3 Nozzle inlet 4 Air flow path 5, 7-9 Chamber 6, 10, 11 Flow path 12 DNA microarray 13 Table 17 Control part 18, 19 Electric syringe pump 20, 21 Pump nozzle 22, 23 Pump block 24 Input unit 25 IC chip 26 Communication unit

Claims (9)

  A biochemical reaction cartridge comprising means for storing information relating to a predetermined process of a biochemical reaction performed internally.   The biochemical reaction cartridge according to claim 1, wherein the predetermined information is a process procedure.   The biochemical reaction cartridge according to claim 1, wherein the predetermined information is a reaction condition of the process.   A biochemical reaction cartridge comprising means for storing information relating to an actual process of a biochemical reaction performed inside.   The biochemical reaction cartridge according to claim 4, wherein the information regarding the actual process is an execution record of the process.   The biochemical reaction cartridge according to claim 1, wherein the means for storing information on the process further stores a method for analyzing the result of the biochemical reaction.   A biochemical reaction cartridge comprising means for storing process identification information corresponding to a predetermined process of an internal biochemical reaction.   8. The biochemical reaction cartridge according to claim 7, further comprising individual identification information for identifying individual biochemical reaction cartridges.   9. The biochemical reaction cartridge according to claim 1, wherein the storage means is any one of a bar code, an IC memory, and a wireless tag.

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