JP2006094741A - Biochemical reaction treatment apparatus and biochemical reaction treatment method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biochemical reaction treatment apparatus that can simply and surely allow a reagent in the chamber of the solution storage part to flow in the chamber of the reaction part. <P>SOLUTION: A valve rod 7 having a notch 8 is inserted into the chamber 6 of the solution storage part 2 including a solution and is held with two O rings 9 and the low part of the solution chamber 6 is closed with aluminum foil 10. In this case, a short pushing bar 13a of the pushing jig 13 is inserted into the chamber 6 to break the aluminum foil 10 and allow the solution to flow in from the chamber 6 to the chamber 11. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a biochemical reaction processing apparatus and a biochemical reaction processing method for analyzing cells, microorganisms, chromosomes, nucleic acids, etc. in a specimen using biochemical reactions such as antigen-antibody reaction and nucleic acid hybridization reaction. It is.

  Many analyzers for specimens such as blood use an immunological method utilizing an antigen-antibody reaction or a method utilizing nucleic acid hybridization. Examples of such analysis methods include using antibodies such as antibodies or antigens that specifically bind to test substances, antigens, or single-stranded nucleic acids as probes, and immobilizing them on solid surfaces such as microparticles, beads, and glass plates. Then, an antigen-antibody reaction or nucleic acid hybridization is performed with the test substance.

  And using a labeling substance having a specific interaction carrying a labeling substance with high detection sensitivity such as an enzyme, a fluorescent substance, a luminescent substance, such as a labeled antibody, a labeled antigen or a labeled nucleic acid, An antigen-antibody compound or double-stranded nucleic acid is detected to detect the presence or absence of a test substance or to quantify the test substance.

  As a development of these techniques, Patent Document 1 describes a so-called DNA array in which a large number of DNA (deoxyribonucleic acid) probes having different base sequences are arranged in an array on a substrate. Non-Patent Document 1 discloses a method for producing a protein array having a structure like a DNA array by arranging many types of proteins on a membrane filter. And it has become possible to perform inspection of a very large number of items at once by using a DNA array, a protein array, or the like.

  In various specimen analysis methods, disposable biochemical reaction cartridges that perform necessary reactions internally have been proposed for the purpose of reducing contamination by specimens, improving reaction efficiency, miniaturizing equipment, and simplifying operations. In Patent Document 2, a plurality of chambers are arranged in a biochemical reaction cartridge including a DNA array, a solution is moved by a differential pressure, and DNA in a sample is extracted or amplified, or hybridized. A biochemical reaction cartridge that enables these reactions is disclosed.

  As a reagent supply method using a biochemical reaction cartridge, Patent Document 3 discloses that a reagent is supplied from an external reagent bottle to a disposable analysis cassette, and Patent Document 2 incorporates a reagent in advance in a chamber. There are disclosures to do.

US Pat. No. 5,445,934 Japanese National Patent Publication No. 11-509094 JP 2000-266759 A Anal. Biochem. 270 (1), 103-111, 1999

  However, when reagents are supplied from the outside, a plurality of reagents must be prepared separately from the biochemical reaction cartridge, and if there are more inspection items, the number of necessary reagents increases and replenishment only becomes troublesome. In addition, there is a possibility that the type of reagent is wrong. In the case of a reagent built in the chamber of the biochemical reaction cartridge, the reagent in the chamber flows into the flow path or other chambers due to environmental changes during storage or transportation, vibration during transportation, etc. Can cause different reactions.

  Resolve the above problems, eliminate the troublesome replenishment of reagents and the wrong type of reagent, and prevent the reagent in the chamber from flowing into the flow path or other chambers even when the environment changes or vibrates during storage or transportation. In order to provide a non-biochemical reaction cartridge, applicants consist of a reaction part with a reaction chamber and a solution storage part with a solution storage chamber for transferring the solution from the solution storage chamber to the chamber of the reaction part In addition, a biochemical reaction cartridge is proposed in which a partition member that can penetrate between the solution storage portion and the reaction portion is provided.

  However, when a plurality of types of solutions are required, the plurality of partition members must be passed through one by one, which is cumbersome, and the work for penetrating the partition members is not performed correctly, so that the solution is reacted. There is a possibility that it cannot move correctly.

  An object of the present invention is to solve the above-described problems and provide a biochemical reaction processing apparatus and a biochemical reaction processing method.

  In order to achieve the above object, the technical feature of the biochemical reaction processing apparatus according to the present invention includes: a reaction part including at least one reaction chamber and a flow path for pouring a plurality of solutions together; A plurality of partition members separately including a solution storage portion including a plurality of storage chambers for storing solutions at positions corresponding to the reaction chamber, and penetrating between the reaction portion and the solution storage portion In the biochemical reaction cartridge provided with the reaction chamber, a valve rod is provided in the solution storage portion, and the reaction chamber corresponding to the solution in the storage chamber passes through the plurality of partition members simultaneously by pushing the valve rod. It is provided with a solution moving means that moves to the position.

  The technical feature of the biochemical reaction processing method according to the present invention is that the reaction part includes at least one reaction chamber and a flow path for pouring a plurality of solutions together, and the reaction part is separated or separated from the reaction part. A biochemical reaction cartridge having a solution storage portion including a plurality of storage chambers for storing a solution at a position corresponding to the chamber, and having a plurality of partition members penetrating between the reaction portion and the solution storage portion In the method, the plurality of partition members are simultaneously penetrated to move the solutions in the plurality of storage chambers to the corresponding reaction chambers.

  According to the biochemical reaction processing apparatus and the biochemical reaction processing method of the present invention, a plurality of partition members can be penetrated through a series of operations, and the reagents in the plurality of solution storage chambers can be easily and reliably moved. The intended reaction can be caused correctly without flowing into the flow path or other chambers.

  FIG. 1 shows a perspective view of a biochemical reaction cartridge in this embodiment. The cartridge has a two-layer structure of a reaction portion 1 where a reaction is performed and a solution storage portion 2 which is disposed on the cartridge and stores a solution such as a reagent / cleaning agent. The main body of the reaction part 1 and the solution storage part 2 is composed of a synthetic resin such as polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene (ABS) copolymer, polystyrene, polycarbonate, polyester, polyvinyl chloride and the like. Yes. When performing optical measurements on the reactants, the reaction portion 1 requires a transparent or translucent plastic.

  A sample inlet 3 for injecting a sample such as blood using a syringe is provided above the reaction portion 1, and the sample inlet 3 is sealed with a rubber cap. Moreover, in order to move the solution in the reaction part 1, a plurality of nozzle inlets 4 for inserting a nozzle and performing pressurization or decompression are provided on both side surfaces of the reaction part 1. Is sealed with a rubber cap, and the opposite surface has the same configuration.

  Further, for example, three aluminum foil sheets 5 are attached to the upper part of the solution storage part 2 in order to close the upper part of the solution storage chamber described later. The reaction portion 1 and the solution storage portion 2 are welded by ultrasonic waves. In addition, the reaction part 1 and the solution storage part 2 may be configured separately and overlapped when used.

  FIG. 2 shows a plan cross-sectional view of the solution storage part 2 of FIG. 1, wherein independent chambers 6a to 6m containing the solution are provided, and the chambers 6a and 6b each include a first hemolytic agent containing EDTA that breaks the cell wall, A second hemolytic agent containing a protein denaturant such as a surfactant is accumulated. Silica-coated magnetic particles that adsorb DNA are accumulated in the chamber 6c, and the first and second extraction detergents used for purifying the DNA during DNA extraction are stored in the chambers 6l and 6m. Is accumulated.

  The chamber 6d has an eluate composed of a low-concentration salt buffer that elutes DNA from magnetic particles, and the chamber 6g has a primer, polymerase, dNTP solution, buffer, and a mixed solution such as Cy-3dUTP containing a fluorescent agent. Is filled. The chambers 6h and 6j have a cleaning agent containing a detergent for washing the fluorescently labeled sample DNA and fluorescent label, and the chamber 6i has a chamber containing a DNA microarray to be described later dried. Because of the accumulation of alcohol. Each chamber 6a to 6m is provided with a pointed valve rod 7a to 7m for penetrating a sheet to be described later.

  FIG. 3 is a cross-sectional view showing the state of the biochemical reaction cartridge during storage. A valve stem 7 having a notch 8 is inserted into the chamber 6 of the solution storage portion 2 containing the solution, and two O-rings. 9 is held. The lower part of the chamber 6 is closed with an aluminum foil sheet 10, and a sealing material 12 is interposed between the chamber 6 and the chamber 11 of the reaction portion 1 to prevent air from entering and exiting. Changes in the volume and pressure of the solution and air due to the environment can be absorbed by the deformation of the aluminum foil sheet 10, and the solution in the chamber 6 does not enter the reaction portion 1 at any time.

  FIG. 4 is a perspective view of a valve rod pushing jig 13 for simultaneously pushing a large number of valve rods 7 into the chamber 11, and the surface of the valve rod pushing jig 13 has positions corresponding to the solution chambers 6a to 6m. A large number of short pressing rods 13a for penetrating the partition member are provided. Although not shown in FIG. 4, a long pressing rod 13b for sealing is provided on the back surface at the same position. In order to inject the solution in the chamber 6 into the chamber 11, the inspector first sets the pressing rod 13a of the valve rod pressing jig 13 so as to contact the chamber 6 in which the respective solutions are put.

  In FIG. 5, a tester injects a liquid sample such as blood from the sample inlet 3 and sets it in a processing apparatus to be described later, and then inserts a short pressing rod 13 a provided in the valve rod pressing jig 13 into the chamber 6. The valve rod 7 is inserted downward, the aluminum foil sheet 10 is broken by the tip of the valve rod 7, and the solution starts to move from the chamber 6 to the chamber 11. In this state, the notch 8 provided in the valve stem 7 communicates with the upper and lower sides of the two O-rings 9, so that air can be passed between the chamber 6 and the outside, and the solution can smoothly enter the chamber 6 to the chamber 11. Can move.

  Thus, since the aluminum foil sheet 10 that can be penetrated as a partition member is provided at the lower part of the chamber 6, the solution can be very easily transferred from the chamber 6 to the chamber by simply pressing the pressing rod 13a into the reaction portion 1 side of the cartridge. 11 can be poured. In this embodiment, there is a chamber 11 of the reaction portion 1 corresponding to a position immediately below the position of the chamber 6 of the solution storage portion 2, but the corresponding chamber 11 is not directly below the chamber 6 by forming a flow path or the like. There is no hindrance.

  Next, the inspector lifts the valve rod pushing jig 13 and temporarily pulls out the short pushing rod 13a, and as shown in FIG. 6, the second stage is formed by the long pushing rod 13b arranged on the back surface of the valve rod pushing jig 13. By pushing in, the valve stem 7 is further pushed down. Then, the air is sealed by the upper O-ring 9 so that the solution can move within the reaction portion 1 as described later. The inspector performs this process simultaneously on all the chambers 6a to 6m. Thus, since the solution can be poured by the first push using the short push rod 13a and sealed by the second push using the long push rod 13b, the solution can be introduced into the chamber 11 by a simple operation of pushing. Two operations of pouring and sealing can be performed. Note that the pressing rods 13 a and 13 b may not be provided on the same pressing jig 13, but two dedicated pressing jigs provided separately may be created.

  Alternatively, the short pressing rod 13a and the long pressing rod 13b may not be provided separately, but only the long pressing rod may be used, and the pressing depth of the pressing rod may be operated in two steps.

  FIG. 7 is a plan sectional view of the reaction portion 1. Ten nozzle inlets 4a to 4j are provided on one side surface, and ten nozzle inlets 4k to 4t are provided on the opposite side surface. The nozzle inlets 4a to 4t communicate with chambers 11a to 11t, which are places where solutions are stored or where reactions occur, via air flow paths 14a to 14t through which air flows. However, since the nozzle inlets 4n, 4p, 4q, and 4s are not used in the process of this embodiment, they are not communicated with the chamber 11 and are reserved.

  That is, the nozzle inlets 4a to 4j communicate with the chambers 11a to 11j via the flow paths 14a to 14j, and the nozzle inlets 4k, 4l, 4m, 4o, 4r, and 4t on the opposite side are respectively connected to the flow paths 14k and 14l. , 14m, 14o, 14r, and 14t communicate with the chambers 11k, 11l, 11m, 11o, 11r, and 11t.

  The specimen inlet 3 communicates with the chamber 16, the chambers 11a, 11b, 11c, and 11k communicate with the chamber 16, the chambers 11g and 11o communicate with the chamber 17, and the chambers 11h, 11i, 11j, 11r, and 11t communicate with the chamber 18. ing. Further, the chamber 16 communicates with the chamber 17 via the flow path 19, and the chamber 17 communicates with the chamber 18 via the flow path 20. Chambers 11d, 11e, 11f, 11l, and 11m communicate with the flow path 19 through flow paths 15d, 15e, 15f, 15l, and 15m, respectively.

  A square hole is formed in the bottom surface of the chamber 18, and a DNA microarray 21 made of a glass substrate is attached to the square hole with the probe surface facing upward. In the DNA microarray 21, different types of DNA probes are arranged in a high density of several hundreds to several hundreds of thousands on a solid phase surface such as a glass plate of about 1 inch square. By performing a hybridization reaction with the sample DNA using this DNA microarray 21, a large number of genes can be examined at once. Further, these DNA probes are regularly arranged in a matrix, and there is an advantage that the addresses (how many rows and what columns) of each DNA probe can be easily taken out as information. In addition to infectious disease viruses / bacteria and disease-related genes, genes to be tested include individual gene polymorphisms.

  In the chambers 11a and 11b of the reaction portion 1, the first hemolytic agent and the second hemolytic agent that have flowed from the chambers 6a and 6b of the solution storage portion 2 by the action of the valve rod 7 are accumulated. The magnetic particles flowing from the chamber 6c are accumulated in the chamber 11c, and the first and second extracted cleaning agents flowing from the chamber 6l and the chamber 6m are accumulated in the chamber 11l and the chamber 11m, respectively. The chamber 11d is filled with the eluate flowing from the chamber 6d, and the chamber 11g is filled with a mixture necessary for PCR (Polymerase Chain Reaction) flowing from the chamber 6g. The chambers 11h and 11j store the cleaning agent that flows from the chambers 6h and 6j, respectively, and the chamber 11i stores the alcohol that flows from the chamber 6i.

  The chamber 11e is a chamber for collecting dust other than blood DNA, the chamber 11f is a chamber for collecting waste liquids of the first and second extraction detergents in the chambers 11l and 11m, and the chamber 11r is for the first and second detergents. The chambers 11k, 11o, and 11t are blank chambers provided to prevent the solution from flowing into the nozzle inlet 4.

  FIG. 8 shows a processing apparatus for controlling the movement of the solution and various reactions in the biochemical reaction cartridge. The table 22 is a place where a biochemical reaction cartridge is set. On the table 22, an electromagnet 23 that is operated when extracting DNA or the like from the sample in the cartridge, and amplifying the DNA from the sample by a method such as PCR. When performing hybridization between the Peltier element 24 for temperature control, the amplified sample DNA and the DNA probe on the DNA microarray in the cartridge, and when washing the sample DNA that has not been hybridized A Peltier element 25 for temperature control is disposed, and these are connected to a control unit 26 for controlling the entire processing apparatus.

  8 is an electric syringe pump 27, 28, and a pump block in which a plurality of pump nozzles 29, 30 are provided on each side at the inlet / outlet for discharging or sucking air by these pumps 27, 28. 31 and 32 are arranged. In addition, the control unit 26 is connected to an input unit 33 that is input by an inspector.

  A plurality of well-known electric switching valves (not shown) are arranged between the electric syringe pumps 27 and 28 and the pump nozzles 29 and 30, and are connected to the control unit 26 together with the pumps 27 and 28. The control unit 26 can control to selectively open one pump nozzle 29, 30 out of 10 on each side with respect to the pumps 27, 28, or to close all pump nozzles 29, 30. Has been.

  When the solution is moved from the solution storage part 2 to the reaction part 1 and a processing start signal is input, DNA and the like are extracted and amplified in the reaction part 1. Hybridization is performed with a DNA probe on a certain DNA microarray, and the fluorescence-labeled sample DNA that has not been hybridized and the fluorescence label are washed.

  In the present embodiment, when blood is used as a specimen and the examiner passes the rubber cap of the specimen inlet 3 through the syringe and injects blood, the blood flows into the chamber 16. Thereafter, the inspector places the biochemical reaction cartridge on the table 22, operates a lever (not shown), and moves the pump blocks 31 and 32 in the direction of the arrow in FIG. 8 to penetrate the rubber cap. Then, pump nozzles 29 and 30 are inserted into the nozzle inlet 4 of the reaction portion 1.

  As shown in FIG. 7, since the nozzle inlet 4 is concentrated on the two surfaces on both sides of the biochemical reaction cartridge, the pump block 31 including the electric syringe pumps 27 and 28, the electric switching valve, and the pump nozzles 29 and 30 is built-in. , 32 is simple in shape and arrangement. Furthermore, the pump nozzles 29 and 30 can be inserted by a simple operation of sandwiching the cartridges at the same time with the pump blocks 31 and 32 while securing the necessary chambers and flow paths. It will be easy. And if all the nozzle inlets 4a-4t are arrange | positioned at the same height, ie, linear form, all the height of the flow paths 14a-14t connected to the nozzle inlets 4a-4t will become the same, and preparation of the flow paths 14a-14t will be carried out. It becomes easy.

  In the processing apparatus of FIG. 8, if the pump blocks 31 and 32 are made to be n times longer for n biochemical reaction cartridges, n cartridges are arranged in series, so that n cartridges are arranged in series. Necessary steps can be performed simultaneously, and a biochemical reaction can be performed on a large number of cartridges with a very simple configuration.

  FIG. 9 is a flowchart for explaining the processing procedure. In step S0, the inspector performs the process of pouring and sealing the solution described with reference to FIGS. 5 and 6 and then inputs a processing start command at the input unit 33 to start the processing. Of course, a robot arm may be provided on the apparatus side, the valve rod pushing jig 13 may be attached to the robot arm, and the steps described with reference to FIGS. 5 and 6 may be performed automatically. By operating the electric syringe pumps 27 and 28, the solution can be poured smoothly.

  In step S1, the control unit 26 opens only the nozzle inlets 4a and 4k, discharges air from the electric syringe pump 27, sucks air from the pump 28, and supplies the first hemolytic agent in the chamber 11a to the chamber containing blood. Pour into 16. At this time, depending on the viscosity of the hemolytic agent and the resistance of the flow path, if the suction of the air from the pump 28 is controlled to start 10 to 200 milliseconds after the start of the discharge of the air from the pump 27, at the head of the flowing solution The solution flows smoothly without splashing out.

  As described above, by controlling the pressurization and decompression by shifting the air supply and suction timings, the solution can flow smoothly. However, the suction of air from the electric syringe pump 28 If fine control such as increasing linearly from the start of discharge is performed, it becomes possible to flow more smoothly. Further, by using both pressurization and depressurization, the pressure generated in the reaction portion 1 can be reduced. As a result, there is a branch channel or chamber in which the solution is not desired to flow during the movement of the solution. In addition, it has the effect of preventing it from flowing into it. The same applies to the movement of the following solutions.

  Control of the air supply can be easily realized by using the electric syringe pumps 27 and 28. Only the nozzle inlets 4a and 4o are opened, and the discharge and suction of air are alternately repeated by the syringe pumps 27 and 28. The operation of flowing the solution into the flow path 19 and returning it to the flow path 19 is repeated to perform stirring. Alternatively, the air may be continuously discharged from the pump 28 and stirred while generating bubbles.

  FIG. 10 is a cross-sectional view of the reaction portion 1 passing through the chamber 11a, the chamber 16, and the chamber 11k of FIG. 7, and the pump nozzle 29 is inserted into the nozzle inlet 4a and pressurized, and the pump nozzle 30 is inserted into the nozzle inlet 4k. The pressure is reduced and the first hemolytic agent in the chamber 11a flows into the chamber 16 containing blood. The chamber 11a actually communicates with the solution storage portion 2, but for convenience, a ceiling is provided and is not communicated.

  Further, in the flowchart of FIG. 9, only the nozzle inlets 4b and 4k are opened in the next step S2, and the second hemolytic agent in the chamber 11b is poured into the chamber 16 in the same manner. In step S3, only the nozzle inlets 4c and 4k are opened. Similarly, the magnetic particles in the chamber 11 c are poured into the chamber 16. In steps S2 and S3, stirring is performed in the same manner as in step S1. In step S3, the DNA produced by the dissolution of the cells in steps S1 and S2 adheres to the magnetic particles.

  Then, when the electromagnet 23 is turned on in step S4, only the nozzle inlets 4e and 4k are opened, the air is discharged from the electric syringe pump 28, the air is sucked from the pump 27, and the solution in the chamber 16 is moved to the chamber 11e. During this movement, magnetic particles and DNA are captured on the electromagnet 23 of the flow path 19. The suction and discharge of the electric syringe pumps 27 and 28 are alternately repeated, and the solution is reciprocated twice between the chambers 16 and 11e to increase the DNA capture efficiency. Increasing the number of times further increases the capture efficiency, but increases the processing time.

  In this way, DNA is captured in a flowing state on a small channel having a width of about 1 to 2 mm and a height of about 0.2 to 1 mm using magnetic particles, so that it can be captured extremely efficiently. Can do. The same applies when the capture target substance is RNA or protein.

  Next, in step S5, the electromagnet 23 is turned off, only the nozzle inlets 4f and 4l are opened, the air is discharged from the electric syringe pump 28, the air is sucked from the pump 27, and the first extraction cleaning liquid in the chamber 11l is supplied to the chamber. Move to 11f. At this time, the magnetic particles and DNA captured in step S4 move together with the extraction cleaning liquid, and cleaning is performed. After reciprocating twice in the same manner as in step S4, the electromagnet 23 is turned on, and reciprocating twice in the same manner to collect the magnetic particles and DNA on the electromagnet 23 in the flow path 19, and return the solution to the chamber 11l. deep.

  In step S6, the second extraction cleaning liquid in the chamber 11m is further cleaned by performing the same process as in step S5 using the nozzle inlets 4f and 4m. In step S7, with the electromagnet 23 turned on, only the nozzle inlets 4d and 4o are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the eluate in the chamber 11d is moved to the chamber 17 To do.

  At this time, the magnetic particles and DNA are separated by the action of the eluate, and only the DNA moves to the chamber 17 together with the eluate, and the magnetic particles remain in the flow path 19, thus performing DNA extraction and purification. Is called. By preparing the chambers 11l and 11m containing the extraction washing liquid and the chamber 11f containing the washing waste liquid, it becomes possible to extract and purify the DNA in the biochemical reaction cartridge 1.

  Next, in step S8, only the nozzle inlets 4g and 4o are opened, the air is discharged from the electric syringe pump 27, the air is sucked from the pump 28, and the PCR drug in the chamber 11g is poured into the chamber 17. Further, only the nozzle inlets 4g and 4t are opened, and the electric syringe pumps 27 and 28 alternately discharge and suck air, and the solution in the chamber 16 is allowed to flow through the flow path 20, and then the operation of returning to it is repeated for stirring. Do. Then, after controlling the Peltier element 24 and holding the solution in the chamber 17 at a temperature of 96 ° C. for 10 minutes, the process of 96 ° C. · 10 seconds, 55 ° C. · 10 seconds, 72 ° C. · 1 minute is repeated 30 times. The amplified DNA is amplified by PCR.

  In step S9, only the nozzle inlets 4g and 4t are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the solution in the chamber 17 is moved to the chamber 18. Further, the Peltier element 25 is controlled to perform hybridization by keeping the solution in the chamber 18 at 45 ° C. for 2 hours. At this time, the pumps 27 and 28 alternately discharge and suck air, and move the solution in the chamber 18 to the flow path 15t, and then repeat the operation of returning to the subsequent, and the hybridization proceeds.

  Next, while maintaining the same 45 ° C. in step S10, this time, only the nozzle inlets 4h and 4r are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the solution in the chamber 18 is discharged. While moving to the chamber 11r, the first cleaning liquid in the chamber 11h is poured into the chamber 11r through the chamber 18. By alternately repeating suction and discharge of the pumps 27 and 28, the solution is reciprocated twice between the chambers 11h, 18 and 11r, and finally returned to the chamber 11h. In this way, the fluorescently labeled sample DNA and the fluorescent label that have not been hybridized are washed.

  FIG. 11 is a cross-sectional view passing through the chamber 11h, the chamber 18, and the chamber 11r of FIG. 7. The pump nozzle 29 is inserted into the nozzle inlet 4h and pressurized, the pump nozzle 30 is inserted into the nozzle inlet 4r, and the pressure is reduced. A state in which the first cleaning liquid in the chamber 11h flows into the chamber 11r through the chamber 18 is shown. The chamber 11h actually communicates with the solution storage portion 2, but for convenience, a ceiling is provided and is not communicated.

  In step S11, while maintaining the same 45 ° C., the second cleaning liquid in the chamber 11j is further cleaned using the nozzle inlets 4j and 4r by performing the same process as in step S10, and finally returned to the chamber 11j. Thus, since the chambers 11h and 11j containing the cleaning liquid and the chamber 11r containing the cleaned waste liquid are prepared, the DNA microarray 21 can be cleaned in the biochemical reaction cartridge.

  In step S12, only the nozzle inlets 4i and 4r are opened, air is discharged from the electric syringe pump 27, air is sucked from the pump 28, and the alcohol in the chamber 11i is moved through the chamber 18 to the chamber 11r. Thereafter, only the nozzle inlets 4i and 4t are opened, air is discharged from the pump 27, air is sucked from the pump 28, and the inside of the chamber 18 is dried.

  Next, when the inspector operates a lever (not shown), the pump blocks 31 and 32 are moved away from the biochemical reaction cartridge by a mechanism (not shown), and the pump nozzles 29 and 30 are detached from the nozzle inlet 4 of the cartridge. Then, the examiner inserts this cartridge into a DNA microarray reader such as a well-known scanner (not shown) to perform measurement and analysis.

It is a perspective view of the biochemical reaction cartridge of an Example. It is a plane sectional view of a solution storage part. It is a partial cross section figure of a biochemical reaction cartridge at the time of preservation. It is a perspective view of a valve stem pushing jig. It is sectional drawing of a part of biochemical reaction cartridge of the state which pushed in the valve stem. It is a partial cross section figure of the biochemical reaction cartridge of the state which pushed the valve rod further. It is a plane sectional view of a reaction part. It is a block block diagram of the processing apparatus which controls the movement of a solution and various reaction. It is a flowchart figure of a processing procedure. It is sectional drawing of a one part chamber. It is sectional drawing of a one part chamber.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Reaction part 2 Solution storage part 3 Sample inlet 4 Nozzle inlet 5, 10 Aluminum foil sheet 7 Valve rod 8 Notch 9 O-ring 11, 16-18 Chamber 13 Valve rod pushing jig | tool 13a, 13b Press rod 15, 19, 20 Channel 21 DNA microarray 22 Table 26 Control unit 27, 28 Electric syringe pump 29, 30 Pump nozzle 31, 32 Pump block

Claims (5)

  A reaction portion including at least one reaction chamber into which a plurality of solutions are poured together and a flow path; and a plurality of storage chambers that store the solution in a position corresponding to the reaction chamber in isolation or separate from the reaction portion. A biochemical reaction cartridge comprising a plurality of partition members that can pass between the reaction portion and the solution storage portion, wherein the solution storage portion is provided with a valve rod, A biochemical reaction processing apparatus, comprising: a solution moving means for simultaneously penetrating the plurality of partition members by pushing and moving the solution in the storage chamber to the corresponding reaction chamber.   The biochemical reaction processing apparatus according to claim 1, further comprising a sealing means for further pressing the valve rod and sealing the reaction chamber from outside air by the valve rod.   The biochemical reaction processing apparatus according to claim 1 or 2, wherein the valve rod is operated by a pressing rod that is pushed in from the outside of the cartridge.   The pressing rod has two types of long and short, the partition member is penetrated by the valve rod using the short pressing rod, and the reaction chamber is sealed from outside air by the valve rod using the long pressing rod. The biochemical reaction processing apparatus according to claim 3.   A reaction portion including at least one reaction chamber into which a plurality of solutions are poured together and a flow path; and a plurality of storage chambers that store the solution in a position corresponding to the reaction chamber in isolation or separate from the reaction portion. A biochemical reaction cartridge having a plurality of partition members penetrating between the reaction portion and the solution storage portion, and simultaneously penetrating the plurality of partition members, A biochemical reaction processing method, wherein the solution in the storage chamber is moved to the corresponding reaction chamber.

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