JP2004261102A5 - - Google Patents

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JP2004261102A5
JP2004261102A5 JP2003055409A JP2003055409A JP2004261102A5 JP 2004261102 A5 JP2004261102 A5 JP 2004261102A5 JP 2003055409 A JP2003055409 A JP 2003055409A JP 2003055409 A JP2003055409 A JP 2003055409A JP 2004261102 A5 JP2004261102 A5 JP 2004261102A5
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ester bond
containing plastic
adipate
enzyme
polyethylene
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JP2003055409A
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JP2004261102A (en
JP4117646B2 (en
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バークホルデリア属に属し、エステル結合含有プラスチック分解能を有する微生物、またはその変異株。  A microorganism belonging to the genus Burkholderia and having a resolution of ester-containing plastics, or a mutant thereof. バークホルデリア属に属する微生物がバークホルデリア セパシアである、請求項１記載の微生物。  The microorganism according to claim 1, wherein the microorganism belonging to the genus Burkholderia is Burkholderia cepacia. バークホルデリア属に属する微生物が、バークホルデリア セパシアＴＢ−６２株、バークホルデリア セパシアＴＢ−６３株、またはバークホルデリア セパシアＴＢ−６５株である、請求項１記載の微生物。  The microorganism according to claim 1, wherein the microorganism belonging to the genus Burkholderia is the Burkholderia cepacia TB-62 strain, the Burkholderia cepacia TB-63 strain, or the Burkholderia cepacia TB-65 strain. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項１乃至３のいずれか一項に記載の微生物。The microorganism according to any one of claims 1 to 3 , wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項１乃至３のいずれか一項に記載の微生物。The microorganism according to any one of claims 1 to 3, wherein the ester bond-containing plastic is polyurethane. 請求項１乃至３のいずれか一項に記載の微生物をエステル結合含有プラスチックと接触させる工程を含む、エステル結合含有プラスチックの分解方法。A method for decomposing an ester bond-containing plastic, comprising the step of bringing the microorganism according to any one of claims 1 to 3 into contact with the ester bond-containing plastic. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項６記載の分解方法。  The decomposition method according to claim 6, wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項６記載の分解方法。  The decomposition method according to claim 6, wherein the ester bond-containing plastic is polyurethane. バークホルデリア属の微生物由来エステル結合含有プラスチック分解酵素を生産する方法であって、
バークホルデリア属の微生物を培養する工程：
前記培養物から前記酵素を分離・精製する工程：
からなる、前記酵素の生産方法。A method for producing an ester bond-containing plastic degrading enzyme derived from a microorganism of the genus Burkholderia,
Steps for culturing Burkholderia microorganisms:
Separating and purifying the enzyme from the culture:
A method for producing the enzyme, comprising: 配列番号１乃至３のいずれかに示すペプチド配列、または該ペプチド配列に１ないし数個のアミノ酸の、欠失、置換、挿入または付加を有するペプチド配列であって、エステル結合含有プラスチック分解酵素活性を維持したペプチド配列からなる、エステル結合含有プラスチック分解酵素。  A peptide sequence shown in any one of SEQ ID NOs: 1 to 3, or a peptide sequence having a deletion, substitution, insertion or addition of 1 to several amino acids in the peptide sequence, wherein the ester bond-containing plastic degrading enzyme activity is An ester bond-containing plastic degrading enzyme comprising a maintained peptide sequence. 請求項１０に記載のエステル結合含有プラスチック分解酵素であって、配列番号１乃至３のいずれかに示すペプチド配列の最初のアミノ酸から１９番目アミノ酸までが欠失したペプチド配列、または該ペプチド配列に１ないし数個のアミノ酸の、欠失、置換、挿入または付加を有するペプチド配列であって、エステル結合含有プラスチック分解酵素活性を維持したペプチド配列からなる、エステル結合含有プラスチック分解酵素。  11. The ester bond-containing plastic degrading enzyme according to claim 10, wherein the peptide sequence shown in any one of SEQ ID NOs: 1 to 3 is deleted from the first amino acid to the 19th amino acid, or 1 in the peptide sequence. An ester bond-containing plastic degrading enzyme comprising a peptide sequence having deletions, substitutions, insertions or additions of several amino acids and maintaining the ester bond-containing plastic degrading enzyme activity. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項１０または１１記載の酵素。  The enzyme according to claim 10 or 11, wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項１０または１１記載の酵素。  The enzyme according to claim 10 or 11, wherein the ester bond-containing plastic is polyurethane. （ａ）、（ｂ）、または（ｃ）に示す塩基配列を含む、ポリヌクレオチド：
（ａ）配列番号１乃至３のいずれかに示すアミノ酸配列、または該アミノ酸配列の最初のアミノ酸から１９番目アミノ酸までが欠失したアミノ酸配列をコードするポリヌクレオチド、またはそれらの相補配列からなるポリヌクレオチド；
（ｂ）ポリエステル分解活性を有するポリペプチドをコードし（ａ）の塩基配列の一部が欠失、置換、挿入若しくは付加された配列、またはその相補配列からなるポリヌクレオチド；
（ｃ）ストリンジェントな条件下で（ａ）または（ｂ）の塩基配列にハイブリダイズする、ポリヌクレオチド。A polynucleotide comprising the base sequence shown in (a), (b), or (c):
(A) a polynucleotide encoding the amino acid sequence shown in any one of SEQ ID NOs: 1 to 3, or an amino acid sequence from which the first amino acid to the 19th amino acid is deleted, or a polynucleotide comprising a complementary sequence thereof ;
(B) a polynucleotide encoding a polypeptide having polyester-degrading activity and comprising a sequence in which a part of the base sequence of (a) is deleted, substituted, inserted or added, or a complementary sequence thereof;
(C) A polynucleotide that hybridizes with the nucleotide sequence (a) or (b) under stringent conditions. 配列番号４乃至６のいずれかに示される塩基配列を有する、ポリヌクレオチド。  A polynucleotide having the base sequence shown in any one of SEQ ID NOs: 4 to 6. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項１４または１５記載のポリヌクレオチド。  The polynucleotide according to claim 14 or 15, wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項１４または１５記載のポリヌクレオチド。  The polynucleotide according to claim 14 or 15, wherein the ester bond-containing plastic is polyurethane. 請求項１０または１１記載の酵素をエステル結合含有プラスチックと接触させる工程を含む、エステル結合含有プラスチックの分解方法。  A method for decomposing an ester bond-containing plastic comprising the step of contacting the enzyme according to claim 10 or 11 with an ester bond-containing plastic. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項１８記載の方法。  19. The method of claim 18, wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項１８記載の方法。  The method according to claim 18, wherein the ester bond-containing plastic is polyurethane. 請求項１０または１１記載の酵素を発現する遺伝子組換え細胞をエステル結合含有プラスチックと接触させる工程を含む、エステル結合含有プラスチックの分解方法。  A method for decomposing an ester bond-containing plastic, comprising a step of bringing a genetically modified cell expressing the enzyme according to claim 10 or 11 into contact with the ester bond-containing plastic. 該細胞が大腸菌である、請求項２１記載のエステル結合含有プラスチックの分解方法。  The method for decomposing an ester bond-containing plastic according to claim 21, wherein the cell is Escherichia coli. エステル結合含有プラスチックがポリブチレンアジペート、ポリエチレンアジペート、ポリエチレンブチレンアジペート、またはポリジエチレンアジペートである、請求項２１または２２記載の方法。  The method according to claim 21 or 22, wherein the ester bond-containing plastic is polybutylene adipate, polyethylene adipate, polyethylene butylene adipate, or polydiethylene adipate. エステル結合含有プラスチックがポリウレタンである、請求項２１または２２記載の方法。  The method according to claim 21 or 22, wherein the ester bond-containing plastic is polyurethane. 遺伝子組換え酵素の生産方法であって、
（ｉ）請求項１０または１１記載の酵素をコードしているポリヌクレオチド配列を含むベクターを含有する宿主細胞を、該宿主細胞の生育に適した条件下で培養する工程；
（ｊ）該酵素を該培養物から分離・精製する工程；
を含む、遺伝子組換え酵素の生産方法。A method for producing a recombinant enzyme comprising:
(I) a step of culturing a host cell containing a vector comprising a polynucleotide sequence encoding the enzyme according to claim 10 or 11 under conditions suitable for the growth of the host cell;
(J) separating and purifying the enzyme from the culture;
A method for producing a recombinant enzyme, comprising: 生分解性を評価すべきポリエステルを、請求項１乃至３のいずれかに記載の微生物、請求項１０または１１に記載の酵素、または請求項１０あるいは１１記載の酵素を発現する遺伝子組換え細胞と接触させて、該ポリエステルの分解程度によりポリエステルの生分解性を評価する方法。  Polyester to be evaluated for biodegradability is a microorganism according to any one of claims 1 to 3, an enzyme according to claim 10 or 11, or a recombinant cell expressing the enzyme according to claim 10 or 11. A method of contacting and evaluating the biodegradability of the polyester by the degree of degradation of the polyester.
JP2003055409A 2003-03-03 2003-03-03 An ester bond-containing plastic-degrading microorganism, a plastic-degrading enzyme, and a polynucleotide encoding the enzyme. Expired - Fee Related JP4117646B2 (en)

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JP2003055409A JP4117646B2 (en) 2003-03-03 2003-03-03 An ester bond-containing plastic-degrading microorganism, a plastic-degrading enzyme, and a polynucleotide encoding the enzyme.

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JP2004261102A5 true JP2004261102A5 (en) 2005-09-02
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US7960154B1 (en) 2005-01-21 2011-06-14 Japan Science And Technology Agency Polyester-based-plastic-degrading bacteria, polyester-based-plastic-degrading enzymes and polynucleotides encoding the enzymes
EP1849859B1 (en) * 2005-01-21 2014-03-12 Japan Science and Technology Agency Polyester plastic-degrading microorganism, polyester plastic-degrading enzyme and polynucleotide encoding the enzyme
JP4670048B2 (en) * 2005-03-03 2011-04-13 国立大学法人 筑波大学 Method for obtaining gene encoding biodegradable plastic degrading enzyme, novel gene and enzyme obtained thereby
JP7096411B2 (en) 2019-03-18 2022-07-05 旭化成メディカル株式会社 Body cavity fluid treatment device
JP7096410B2 (en) 2019-05-22 2022-07-05 旭化成メディカル株式会社 Body cavity fluid treatment device
CN112063295B (en) * 2020-09-04 2022-05-17 宁波爱甬新材料科技有限公司 Low-gloss bending-resistant powder coating and preparation method thereof
WO2024197531A1 (en) * 2023-03-27 2024-10-03 中国科学院深圳先进技术研究院 Engineering bacterium capable of degrading plastic, and degradable living plastic and preparation method therefor and use thereof

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