JP2004231851A

JP2004231851A - Aqueous pigment liquid derived from algae

Info

Publication number: JP2004231851A
Application number: JP2003023685A
Authority: JP
Inventors: Toshimitsu Kato; 敏光加藤; Sachiko Nagatsuka; 佐智子長塚; Hiraki Kubo; 開久保; Masaki Sakakibara; 正樹榊原
Original assignee: Dainippon Ink and Chemicals Co Ltd
Current assignee: DIC Corp
Priority date: 2003-01-31
Filing date: 2003-01-31
Publication date: 2004-08-19

Abstract

<P>PROBLEM TO BE SOLVED: To provide an aqueous pigment liquid derived from algae scarcely causing proliferation of various germs even when stored for a long period at a normal temperature and enabling long-term storage. <P>SOLUTION: The aqueous pigment liquid of a pigment derived from algae comprises preferably 8-40 pts. wt. (expressed in terms of solid content) pigment derived from blue algae such as Spirulina and/or a pigment derived from red algae such as porphyra and preferably 10-40 pts. wt. one or more kinds of compounds selected from a group consisting of ethanol, propylene glycol and glycerol and water. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

【０００１】
【発明の属する技術分野】
本発明は、雑菌が増殖しにくく、長期間保存可能な藻類由来の水性色素液に関する。
【０００２】
【従来の技術】
近年、食品用色素液として、安全性の高い天然物由来の水性色素液が好んで用いられており、天然物由来の水性色素液のなかでも、藻類由来の水性色素液が注目されている。藻類由来の水性色素液は、例えば、スピルリナをｐＨが４〜７の緩衝液に懸濁した懸濁液を遠心分離して残渣を除去して得られる青色の抽出液に硫酸アンモニウムを添加して塩析を行った後、遠心分離して得られた青色色素に水を添加し水溶液とする方法により製造されている（例えば、特許文献１参照。）。
【０００３】
しかしながら、前記特許文献１の製造方法で得られた藻類由来の水性色素液は低温、例えば、４℃で保存しておいても雑菌が増殖しやすく、長期間保存するのは困難であった。
【０００４】
【特許文献１】
特開昭５２−１３４０５８号公報（３〜５頁）
【０００５】
【発明が解決しようとする課題】
本発明の課題は、雑菌が増殖しにくい藻類由来の水性色素液を提供することである。
【０００６】
【課題を解決するための手段】
本発明者らは鋭意検討した結果、藻類由来の水性色素液にアルコールを含有させると雑菌が増殖しにくくなり、長期保存が可能となることを見出し、本発明をなすに至った。
【０００７】
即ち、本発明は、藻類由来色素（Ａ）とアルコール（Ｂ）と水とを含有することを特徴とする藻類由来の水性色素液を提供するものである。
【０００８】
【発明の実施の形態】
以下に本発明をより詳細に説明する。
本発明で用いる藻類由来色素（Ａ）は、調製方法には特に制限が無く、例えば、藻類をｐＨ４〜７の緩衝液に懸濁した懸濁液を遠心分離して残渣を除去した抽出液に、硫酸アンモニウムを添加して塩析を行った後、遠心分離して沈殿物を回収する等の方法により調製できる。藻類由来色素（Ａ）は、粉末のものを使用してもよいし、水で溶解し水溶液となっているものを使用しても良い。また、藻類由来色素（Ａ）は、市販品としては例えば、リナブルーＨＧＥ（大日本インキ化学工業株式会社製のスピルリナ色素の粉末）等が挙げられる。
【０００９】
前記藻類由来色素（Ａ）としては、例えば、藍藻類由来色素、緑藻類由来色素、褐藻類由来色素、紅藻類由来色素、クリプト藻由来色素等が挙げられる。藻類由来色素（Ａ）としては藍藻類由来色素、紅藻類由来色素が好ましい。
【００１０】
前記藍藻類由来色素を得るための藍藻類としては、例えば、スピルリナ属、アファニゾメノン属、フィッシェレラ属、アナベナ属、ネンジュモ属、シネコキスチス属、シネココッカス属、トリポスリクス属、スイゼンジノリ属、マスティゴクラディス属、プルロカプサ属等が挙げられる。前記紅藻類由来色素を得るための紅藻類としては、例えば、ポルフィラ属（アマノリ）、ポルフィロプシス属、ポルフィリディウム属、ゴニオトリクム属、ロリシフォニア属、カルポポルチス属等が挙げられる。
【００１１】
前記藍藻類由来色素（Ａ）としてはスピルリナ属由来色素が、前記紅藻類由来色素液としてはポルフィラ属由来色素が好ましい。
【００１２】
本発明の藻類由来の水性色素液は、藻類由来色素（Ａ）を固形分換算で５〜５０重量％含有しているのが好ましく、８〜４０重量％含有しているのがより好ましく、１０〜３０重量％含有しているのが特に好ましい。
【００１３】
本発明で用いるアルコール（Ｂ）としては、特に制限は無いが、食品用途に安全に使用できるのでエタノール、プロピレングリコール、グリセリンが好ましく使用できる。なかでもグリセリンが、雑菌の増殖を抑制するのに加え、長期保存しても藻類由来の水性色素液の色価が低下しにくいのでより好ましい。
【００１４】
本発明の藻類由来の水性色素液は、アルコール（Ｂ）を１〜６０重量％含有しているのが好ましく、５〜５０重量％含有しているのがより好ましく、１０〜４０重量％含有しているのが特に好ましい。
【００１５】
本発明の藻類由来の水性色素液は、前記藻類由来色素（Ａ）とアルコール（Ｂ）と共に水を含有する。藻類由来の水性色素液１００重量部中の水の含有量としては１０〜９０重量部が好ましく、３０〜８０重量部がより好ましく、３５〜７０重量部が特に好ましい。
【００１６】
本発明の藻類由来の水性色素液は、例えば、以下の方法により調製することができる。
▲１▼藻類由来色素（Ａ）として藻類由来色素の水溶液を用い、該藻類由来色素の水溶液とアルコール（Ｂ）と必要に応じて水とを混合し、攪拌機により攪拌する。これらは同時に混合しても良いし、藻類由来色素の水溶液とアルコール（Ｂ）とを混合した後に必要に応じて水を添加し混合しても良いし、藻類由来色素の水溶液と必要に応じて水を混合した後にアルコール（Ｂ）を添加し混合しても良い。
▲２▼藻類由来色素（Ａ）として粉末の藻類由来色素を用い、該藻類由来色素とアルコール（Ｂ）と水とを混合し、攪拌機により攪拌する。これらは同時に混合しても良いし、粉末の藻類由来色素と水とを混合した後にアルコール（Ｂ）を添加して混合しても良いし、粉末の藻類由来色素とアルコール（Ｂ）とを混合した後に水を添加して混合しても良いし、アルコール（Ｂ）と水と混合した後に粉末の藻類由来色素を添加して混合しても良い。
【００１７】
【実施例】
次に本発明を実施例、比較例により説明する。例中において「％」は特に断りにない限り、重量基準である。
【００１８】
実施例１
リナブルーＨＧＥ（大日本インキ化学工業株式会社製のスピルリナ色素の粉末、色素の含有率：４０％）３２ｇ、水４０ｇおよびグリセリン２８ｇを加えて攪拌し、目視で均一な状態になったことを確認し、青色のスピルリナ由来の水性色素液（１）を得た。
【００１９】
得られたスピルリナ由来の水性色素液（１）の評価（雑菌の増殖試験、色価変化測定試験）を下記方法に従い行った。その結果を第１表に示す。
【００２０】
＜雑菌の増殖試験＞
スピルリナ由来の水性色素液（１）を滅菌済みのキャップ付ガラス容器に入れて、２５℃で７日間静置した。その後、シャーレ上のコロニー数が３０〜３００個になるように滅菌済み生理緩衝食塩水で所定希釈倍数に希釈した試料液１．０ｍｌをシャーレに加え、そこへ加温溶解して５０℃に保った標準寒天培地〔日水製薬（株）製〕１０ｍＬを添加した。シャーレ上で試料液と標準寒天培地を十分混和し、冷却固化した。３５℃で２日間静置し、寒天培地上にできたコロニーの数を数え、その数に希釈倍数を乗じて２５℃で７日間培養したスピルリナ由来の水性色素液（１）１ｇ中の一般生菌数を求めた。一般生菌数が少ないほど、雑菌の増殖が抑制されていることを示す。
【００２１】
＜色価変化測定試験＞
スピルリナ由来の水性色素液（１）を蒸留水で１０００倍希釈し、波長６１８ｎｍの吸光度を分光光度計で測定した。この値を１０倍することでスピルリナ由来の水性色素液（１）の１％水溶液の色価（Ｅ１％）Ａを求めた。同様の操作を滅菌済みキャップ付きガラス容器に入れて、２５℃で７日間静置したスピルリナ由来の水性色素液に対しても行い色価（Ｅ１％）Ｂを求めた。２５℃、７日間静置後の色価残存率（％）を、計算式（Ｂ÷Ａ×１００）から求めた。
【００２２】
実施例２
リナブルーＨＧＥ３２ｇ、水４０ｇおよびプロピレングリコール２８ｇを加えて攪拌し目視で均一な状態になったことを確認し、青色のスピルリナ由来の水性色素液（２）を得た。実施例１と同様にして試験を行い、その結果を第１表に示す。
【００２３】
実施例３
リナブルーＨＧＥ３２ｇ、水５８ｇおよびエタノール１０ｇを加えて攪拌し、目視で均一な状態になったことを確認し、青色のスピルリナ由来の水性色素液（３）を得た。実施例１と同様にして試験を行い、その結果を第１表に示す。
【００２４】
比較例１
リナブルーＨＧＥ３２ｇ、水６８ｇを加えて攪拌した。目視で均一な状態になったことを確認し、青色の比較対照用スピルリナ由来の水性色素液（１′）を得た。実施例１と同様にして試験を行い、その結果を第１表に示す。
【００２５】
【表１】

【００２６】
【発明の効果】
本発明の藻類由来の水性色素液は、雑菌が増殖しにくく長期間の保存が可能である。[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to an aqueous pigment liquid derived from algae, which hardly grows various bacteria and can be stored for a long time.
[0002]
[Prior art]
In recent years, aqueous dye liquids derived from natural products having high safety have been favorably used as dye liquids for food, and among aqueous dye liquids derived from natural products, aqueous dye liquids derived from algae have attracted attention. The aqueous pigment solution derived from algae is, for example, a salt obtained by adding ammonium sulfate to a blue extract obtained by centrifuging a suspension of spirulina in a buffer having a pH of 4 to 7 and removing the residue. It is manufactured by a method in which water is added to a blue dye obtained by centrifuging after performing precipitation to form an aqueous solution (for example, see Patent Document 1).
[0003]
However, the aqueous pigment liquid derived from algae obtained by the production method of Patent Literature 1 easily grows germs even when stored at a low temperature, for example, at 4 ° C., and is difficult to store for a long time.
[0004]
[Patent Document 1]
JP-A No. 52-134,058 (pages 3 to 5)
[0005]
[Problems to be solved by the invention]
An object of the present invention is to provide an aqueous pigment liquid derived from algae in which various bacteria are difficult to grow.
[0006]
[Means for Solving the Problems]
As a result of intensive studies, the present inventors have found that when an alcohol is contained in an aqueous pigment solution derived from algae, it is difficult for bacteria to grow and long-term storage is possible, and the present invention has been accomplished.
[0007]
That is, the present invention provides an aqueous pigment liquid derived from algae, which contains an algal pigment (A), an alcohol (B), and water.
[0008]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, the present invention will be described in more detail.
The algal-derived pigment (A) used in the present invention is not particularly limited in its preparation method. For example, the extract obtained by removing the residue by centrifuging a suspension of algae in a buffer having a pH of 4 to 7 is removed. And salting out by adding ammonium sulfate, followed by centrifugation to collect a precipitate. The algae-derived pigment (A) may be in the form of a powder or may be dissolved in water to form an aqueous solution. Examples of the algae-derived dye (A) include commercially available products such as Lina Blue HGE (a powder of spirulina dye manufactured by Dainippon Ink and Chemicals, Inc.).
[0009]
Examples of the algae-derived pigment (A) include cyanobacterium-derived pigments, green algae-derived pigments, brown algae-derived pigments, red algae-derived pigments, and cryptoalga-derived pigments. Algae-derived pigments (A) are preferably cyanobacteria-derived pigments and red algae-derived pigments.
[0010]
Examples of the cyanobacteria for obtaining the cyanobacteria-derived pigment include, for example, genus Spirulina, Aphanizomenone, Fischerella, Anabena, Nostoc, Synechocystis, Synechococcus, Triphosricus, Suizendinori, Mastigoladis, Purlocapsa And the like. Examples of the red algae for obtaining the red algae-derived pigment include the genus Porphyra (Amanori), the genus Porphyropsis, the genus Porphyridium, the genus Goniotrichum, the genus Loriciphonia, and the genus Carpoportis.
[0011]
The cyanobacterium-derived pigment (A) is preferably a spirulina-derived pigment, and the red algae-derived pigment liquid is preferably a Porphyra-derived pigment.
[0012]
The algae-derived aqueous dye liquid of the present invention preferably contains 5 to 50% by weight, more preferably 8 to 40% by weight, and more preferably 10 to 40% by weight of the algae-derived dye (A) in terms of solid content. It is particularly preferred that the content is 30 to 30% by weight.
[0013]
The alcohol (B) used in the present invention is not particularly limited, but ethanol, propylene glycol and glycerin can be preferably used because they can be used safely for food applications. Among them, glycerin is more preferred because it suppresses the growth of various bacteria and the color value of the aqueous pigment liquid derived from algae does not easily decrease even after long-term storage.
[0014]
The algae-derived aqueous pigment liquid of the present invention preferably contains 1 to 60% by weight of alcohol (B), more preferably 5 to 50% by weight, and more preferably 10 to 40% by weight. Is particularly preferred.
[0015]
The aqueous pigment liquid derived from algae of the present invention contains water together with the algal pigment (A) and the alcohol (B). The content of water in 100 parts by weight of the aqueous pigment liquid derived from algae is preferably 10 to 90 parts by weight, more preferably 30 to 80 parts by weight, and particularly preferably 35 to 70 parts by weight.
[0016]
The aqueous pigment solution derived from algae of the present invention can be prepared, for example, by the following method.
{Circle around (1)} An aqueous solution of an algal-derived pigment is used as the algal-derived pigment (A), and the aqueous solution of the algal-derived pigment, the alcohol (B) and, if necessary, water are mixed, and the mixture is stirred by a stirrer. These may be mixed at the same time, or may be mixed with an aqueous solution of the algal-derived pigment after addition of the aqueous solution of the algal-derived pigment and the alcohol (B), or may be mixed with the aqueous solution of the algal-derived pigment, if necessary. After mixing the water, the alcohol (B) may be added and mixed.
{Circle around (2)} Algae-derived pigment (A) is a powdered algal-origin pigment, and the algae-derived pigment is mixed with alcohol (B) and water, and stirred with a stirrer. These may be mixed at the same time, may be mixed with powdered algal pigment and water and then added with alcohol (B), or may be mixed with powdered algal pigment and alcohol (B) Then, water may be added and mixed, or the alcohol (B) and water may be mixed and then a powdered algal pigment may be added and mixed.
[0017]
【Example】
Next, the present invention will be described with reference to Examples and Comparative Examples. In the examples, “%” is based on weight unless otherwise specified.
[0018]
Example 1
32 g of Lina Blue HGE (powder of spirulina dye manufactured by Dainippon Ink and Chemicals, Inc., content of dye: 40%), 40 g of water and 28 g of glycerin were added and stirred, and it was confirmed that the mixture was visually uniform. Thus, an aqueous dye liquid (1) derived from blue spirulina was obtained.
[0019]
Evaluation of the obtained aqueous dye liquid (1) derived from Spirulina (growth test of various germs, measurement test of color value change) was performed according to the following methods. Table 1 shows the results.
[0020]
<Bacterial growth test>
The aqueous dye solution (1) derived from Spirulina was placed in a sterilized glass container with a cap, and allowed to stand at 25 ° C. for 7 days. Thereafter, 1.0 ml of a sample solution diluted to a predetermined dilution with a sterilized physiological buffer solution was added to the Petri dish so that the number of colonies on the Petri dish was 30 to 300, and the mixture was heated and dissolved therein and kept at 50 ° C. 10 mL of standard agar medium [manufactured by Nissui Pharmaceutical Co., Ltd.] was added. The sample solution and the standard agar medium were sufficiently mixed on a Petri dish, and cooled and solidified. After standing at 35 ° C for 2 days, counting the number of colonies formed on the agar medium, multiplying the number by a dilution factor, and culturing at 25 ° C for 7 days. The number of bacteria was determined. The smaller the number of general viable bacteria, the more the growth of various bacteria is suppressed.
[0021]
<Color value change measurement test>
The aqueous dye solution (1) derived from Spirulina was diluted 1000 times with distilled water, and the absorbance at a wavelength of 618 nm was measured with a spectrophotometer. By multiplying this value by 10 times, the color value (E1%) A of a 1% aqueous solution of the aqueous dye liquid (1) derived from Spirulina was determined. The same operation was performed on a spirulina-derived aqueous dye solution which was placed in a sterilized glass container with a cap and allowed to stand at 25 ° C. for 7 days, to obtain a color value (E1%) B. The color value residual rate (%) after standing at 25 ° C. for 7 days was determined from a calculation formula (B ÷ A × 100).
[0022]
Example 2
32 g of Lina Blue HGE, 40 g of water and 28 g of propylene glycol were added, and the mixture was stirred and visually confirmed to be in a uniform state, whereby a blue aqueous spirulina-derived aqueous dye liquid (2) was obtained. The test was performed in the same manner as in Example 1, and the results are shown in Table 1.
[0023]
Example 3
32 g of Linablue HGE, 58 g of water and 10 g of ethanol were added and stirred, and it was visually confirmed that the state became uniform. Thus, an aqueous dye liquid (3) derived from blue Spirulina was obtained. The test was performed in the same manner as in Example 1, and the results are shown in Table 1.
[0024]
Comparative Example 1
32 g of Linablue HGE and 68 g of water were added and stirred. It was confirmed by visual observation that the state became uniform, and a blue aqueous pigment liquid (1 ') derived from spirulina for comparison was obtained. The test was performed in the same manner as in Example 1, and the results are shown in Table 1.
[0025]
[Table 1]

[0026]
【The invention's effect】
The aqueous pigment liquid derived from algae of the present invention is hard to grow germs and can be stored for a long time.

Claims

An aqueous pigment liquid derived from algae, comprising an algal pigment (A), an alcohol (B) and water.

The aqueous pigment liquid according to claim 1, wherein the algal pigment (A) is a cyanobacterial pigment or a red algal pigment.

The aqueous pigment liquid according to claim 2, wherein the cyanobacterial pigment is a spirulina pigment, and the red algae pigment is a porphyra pigment.

The aqueous pigment liquid according to claim 3, which contains the algal pigment (A) in an amount of 8 to 40% by weight in terms of solid content.

The aqueous dye liquid according to any one of claims 1 to 4, wherein the alcohol (B) is at least one compound selected from the group consisting of ethanol, propylene glycol, and glycerin.

The aqueous dye liquid according to claim 5, comprising 10 to 40% by weight of the alcohol (B).