JP2003530118A5 - - Google Patents
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- Publication number
- JP2003530118A5 JP2003530118A5 JP2001575233A JP2001575233A JP2003530118A5 JP 2003530118 A5 JP2003530118 A5 JP 2003530118A5 JP 2001575233 A JP2001575233 A JP 2001575233A JP 2001575233 A JP2001575233 A JP 2001575233A JP 2003530118 A5 JP2003530118 A5 JP 2003530118A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- sample
- life
- cells
- death discrimination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 21
- 238000000034 method Methods 0.000 claims 19
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 108020004707 nucleic acids Proteins 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 17
- 230000034994 death Effects 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- ZJVOHJVSTORGLI-UHFFFAOYSA-N 5-Methylangelicin Chemical compound O1C(=O)C=CC2=C1C(C=CO1)=C1C=C2C ZJVOHJVSTORGLI-UHFFFAOYSA-N 0.000 claims 1
- GHUXAYLZEGLXDA-UHFFFAOYSA-N 8-azido-5-ethyl-6-phenylphenanthridin-5-ium-3-amine;bromide Chemical compound [Br-].C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 GHUXAYLZEGLXDA-UHFFFAOYSA-N 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 102000016911 Deoxyribonucleases Human genes 0.000 claims 1
- 108010053770 Deoxyribonucleases Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 230000006037 cell lysis Effects 0.000 claims 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical group [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims 1
- 229960005542 ethidium bromide Drugs 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- 230000002427 irreversible effect Effects 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000001216 nucleic acid method Methods 0.000 claims 1
- 230000001293 nucleolytic effect Effects 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0008787.4 | 2000-04-10 | ||
| GBGB0008787.4A GB0008787D0 (en) | 2000-04-10 | 2000-04-10 | A method of cell detection |
| PCT/GB2001/001619 WO2001077379A2 (en) | 2000-04-10 | 2001-04-10 | A method of cell detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003530118A JP2003530118A (ja) | 2003-10-14 |
| JP2003530118A5 true JP2003530118A5 (https=) | 2005-06-09 |
| JP4340734B2 JP4340734B2 (ja) | 2009-10-07 |
Family
ID=9889587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001575233A Expired - Lifetime JP4340734B2 (ja) | 2000-04-10 | 2001-04-10 | 細胞検出方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7262009B2 (https=) |
| EP (1) | EP1272664A2 (https=) |
| JP (1) | JP4340734B2 (https=) |
| AU (2) | AU4671201A (https=) |
| CA (1) | CA2405769A1 (https=) |
| GB (1) | GB0008787D0 (https=) |
| NO (1) | NO20024879L (https=) |
| WO (1) | WO2001077379A2 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006007479A2 (en) * | 2004-07-01 | 2006-01-19 | Litron Laboratories, Ltd. | Method for enumerating mammalian cell micronuclei via differential staining |
| EP1918385B1 (en) | 2005-07-21 | 2011-06-29 | Morinaga Milk Industry Co., Ltd. | Method for detection of microorganism and kit for detection of microorganism |
| WO2007094077A1 (ja) | 2006-02-17 | 2007-08-23 | Kyushu University | 微生物検出法及び微生物検出キット |
| US8198040B2 (en) | 2006-02-28 | 2012-06-12 | Montana State University | Use of phenanthridium derivatives for distinguishing between live and dead cells |
| JP4113556B2 (ja) * | 2006-12-04 | 2008-07-09 | 国立大学法人九州大学 | 核酸分解剤および核酸の分解方法 |
| AU2008287928B2 (en) | 2007-08-16 | 2011-07-28 | Morinaga Milk Industry Co., Ltd. | Method and kit for detection of microorganism |
| KR101383389B1 (ko) | 2009-07-24 | 2014-04-08 | 모리나가 뉴교 가부시키가이샤 | 미생물 검출법 및 미생물 검출 키트 |
| WO2011043737A1 (en) * | 2009-10-05 | 2011-04-14 | Nanyang Technological University | Viability analysis of protozoa using polymerase chain reaction (pcr) |
| FR2974117B1 (fr) | 2011-04-18 | 2018-05-11 | Societe Des Eaux De Marseille | Procede de quantification de bacteries vivantes dans un milieu liquide |
| CA2872149C (en) | 2012-05-02 | 2019-03-19 | Charles River Laboratories, Inc. | Viability staining method |
| CN104755931B (zh) | 2012-05-02 | 2016-08-31 | 查尔斯河实验室公司 | 用于检测细胞样品中的活细胞的方法 |
| CN104685346B (zh) | 2012-05-02 | 2018-05-29 | 查尔斯河实验室公司 | 细胞捕获系统及其使用 |
| DE102020103971B4 (de) | 2020-02-14 | 2022-02-24 | Testo bioAnalytics GmbH | Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem |
| WO2022051405A1 (en) | 2020-09-02 | 2022-03-10 | Promega Corporation | Nucleic acid modifying reagents and uses thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0517154A1 (en) * | 1991-06-06 | 1992-12-09 | F. Hoffmann-La Roche Ag | Method for detecting giardia |
| US5314805A (en) * | 1991-10-28 | 1994-05-24 | Molecular Probes, Inc. | Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM |
| US5525471A (en) * | 1994-10-12 | 1996-06-11 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enzymatic degrading subtraction hybridization |
| US20020119438A1 (en) | 1995-12-29 | 2002-08-29 | Fuminori Kato | Method for determining viable cell count |
| US5780278A (en) * | 1996-05-20 | 1998-07-14 | Vanderbilt University | IceA gene and related methods |
| GB9709728D0 (en) | 1997-05-13 | 1997-07-02 | Dynal As | Single step method |
-
2000
- 2000-04-10 GB GBGB0008787.4A patent/GB0008787D0/en not_active Ceased
-
2001
- 2001-04-10 AU AU4671201A patent/AU4671201A/xx active Pending
- 2001-04-10 US US10/257,493 patent/US7262009B2/en not_active Expired - Fee Related
- 2001-04-10 EP EP01919651A patent/EP1272664A2/en not_active Withdrawn
- 2001-04-10 JP JP2001575233A patent/JP4340734B2/ja not_active Expired - Lifetime
- 2001-04-10 AU AU2001246712A patent/AU2001246712C1/en not_active Ceased
- 2001-04-10 CA CA002405769A patent/CA2405769A1/en not_active Abandoned
- 2001-04-10 WO PCT/GB2001/001619 patent/WO2001077379A2/en not_active Ceased
-
2002
- 2002-10-09 NO NO20024879A patent/NO20024879L/no unknown
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