JP2003509066A - 遺伝学的毒性マーカー、その調製及び使用 - Google Patents
遺伝学的毒性マーカー、その調製及び使用Info
- Publication number
- JP2003509066A JP2003509066A JP2001523800A JP2001523800A JP2003509066A JP 2003509066 A JP2003509066 A JP 2003509066A JP 2001523800 A JP2001523800 A JP 2001523800A JP 2001523800 A JP2001523800 A JP 2001523800A JP 2003509066 A JP2003509066 A JP 2003509066A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- cell
- cells
- bank
- deregulated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 231100000025 genetic toxicology Toxicity 0.000 title claims abstract description 29
- 230000007674 genetic toxicity Effects 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title description 17
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 146
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 140
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 140
- 238000000034 method Methods 0.000 claims abstract description 135
- 150000001875 compounds Chemical class 0.000 claims abstract description 130
- 238000012360 testing method Methods 0.000 claims abstract description 63
- 230000004083 survival effect Effects 0.000 claims abstract description 19
- 239000003550 marker Substances 0.000 claims abstract description 18
- 230000004663 cell proliferation Effects 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 180
- 230000002074 deregulated effect Effects 0.000 claims description 88
- 238000009396 hybridization Methods 0.000 claims description 83
- 108090000623 proteins and genes Proteins 0.000 claims description 83
- 230000002068 genetic effect Effects 0.000 claims description 67
- 230000001988 toxicity Effects 0.000 claims description 67
- 231100000419 toxicity Toxicity 0.000 claims description 67
- 230000014509 gene expression Effects 0.000 claims description 60
- 239000000523 sample Substances 0.000 claims description 54
- 230000003831 deregulation Effects 0.000 claims description 42
- 230000037361 pathway Effects 0.000 claims description 35
- 230000005754 cellular signaling Effects 0.000 claims description 33
- 230000006907 apoptotic process Effects 0.000 claims description 32
- 230000004044 response Effects 0.000 claims description 28
- 102000054765 polymorphisms of proteins Human genes 0.000 claims description 26
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 23
- 239000002299 complementary DNA Substances 0.000 claims description 22
- 108020004414 DNA Proteins 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 18
- 206010028980 Neoplasm Diseases 0.000 claims description 16
- 108700025716 Tumor Suppressor Genes Proteins 0.000 claims description 16
- 102000044209 Tumor Suppressor Genes Human genes 0.000 claims description 16
- 108020004999 messenger RNA Proteins 0.000 claims description 15
- 230000004048 modification Effects 0.000 claims description 14
- 238000012986 modification Methods 0.000 claims description 14
- 230000002588 toxic effect Effects 0.000 claims description 14
- 230000006698 induction Effects 0.000 claims description 13
- 230000035772 mutation Effects 0.000 claims description 13
- 231100000331 toxic Toxicity 0.000 claims description 13
- 230000004913 activation Effects 0.000 claims description 12
- 210000001519 tissue Anatomy 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 230000002103 transcriptional effect Effects 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims description 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 9
- 239000002853 nucleic acid probe Substances 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 230000010261 cell growth Effects 0.000 claims description 8
- 239000012634 fragment Substances 0.000 claims description 8
- 231100000683 possible toxicity Toxicity 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 210000004881 tumor cell Anatomy 0.000 claims description 8
- 239000012472 biological sample Substances 0.000 claims description 7
- 238000002955 isolation Methods 0.000 claims description 7
- 241001465754 Metazoa Species 0.000 claims description 6
- 108091000080 Phosphotransferase Proteins 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 229940079920 digestives acid preparations Drugs 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- 102000020233 phosphotransferase Human genes 0.000 claims description 6
- 238000013518 transcription Methods 0.000 claims description 6
- 230000035897 transcription Effects 0.000 claims description 6
- 108020005196 Mitochondrial DNA Proteins 0.000 claims description 5
- 230000037442 genomic alteration Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 108020003175 receptors Proteins 0.000 claims description 4
- 102000005962 receptors Human genes 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000000018 DNA microarray Methods 0.000 claims description 3
- 108010091356 Tumor Protein p73 Proteins 0.000 claims description 3
- 102000018252 Tumor Protein p73 Human genes 0.000 claims description 3
- 230000002285 radioactive effect Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- AFWRJOYNLMVZQO-GMFATLNBSA-N (1r,2r,4as,8as)-1-[(1e,3e)-5-hydroxy-3-methylpenta-1,3-dienyl]-2,5,5,8a-tetramethyl-3,4,4a,6,7,8-hexahydro-1h-naphthalen-2-ol Chemical compound CC1(C)CCC[C@]2(C)[C@@H](/C=C/C(=C/CO)/C)[C@](C)(O)CC[C@H]21 AFWRJOYNLMVZQO-GMFATLNBSA-N 0.000 claims description 2
- 102000001556 1-Phosphatidylinositol 4-Kinase Human genes 0.000 claims description 2
- 108010029190 1-Phosphatidylinositol 4-Kinase Proteins 0.000 claims description 2
- 102000006589 Alpha-ketoglutarate dehydrogenase Human genes 0.000 claims description 2
- 108020004306 Alpha-ketoglutarate dehydrogenase Proteins 0.000 claims description 2
- 102000004149 Annexin A2 Human genes 0.000 claims description 2
- 108090000668 Annexin A2 Proteins 0.000 claims description 2
- 102100034501 Cyclin-dependent kinases regulatory subunit 1 Human genes 0.000 claims description 2
- 102100034690 Delta(14)-sterol reductase LBR Human genes 0.000 claims description 2
- 102000003817 Fos-related antigen 1 Human genes 0.000 claims description 2
- 108090000123 Fos-related antigen 1 Proteins 0.000 claims description 2
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 claims description 2
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 claims description 2
- 102000012215 HSC70 Heat-Shock Proteins Human genes 0.000 claims description 2
- 108010036652 HSC70 Heat-Shock Proteins Proteins 0.000 claims description 2
- 101001090865 Homo sapiens 26S proteasome regulatory subunit 7 Proteins 0.000 claims description 2
- 101000710200 Homo sapiens Cyclin-dependent kinases regulatory subunit 1 Proteins 0.000 claims description 2
- 101001033293 Homo sapiens Interleukin enhancer-binding factor 3 Proteins 0.000 claims description 2
- 101000707561 Homo sapiens Splicing factor 3A subunit 2 Proteins 0.000 claims description 2
- 101000597043 Homo sapiens Transcriptional enhancer factor TEF-5 Proteins 0.000 claims description 2
- 101000653679 Homo sapiens Translationally-controlled tumor protein Proteins 0.000 claims description 2
- 101000828889 Homo sapiens tRNA modification GTPase GTPBP3, mitochondrial Proteins 0.000 claims description 2
- 102100039062 Interleukin enhancer-binding factor 3 Human genes 0.000 claims description 2
- 102000045959 Interleukin-1 Receptor-Like 1 Human genes 0.000 claims description 2
- 108700003107 Interleukin-1 Receptor-Like 1 Proteins 0.000 claims description 2
- 102100033421 Keratin, type I cytoskeletal 18 Human genes 0.000 claims description 2
- 108010066327 Keratin-18 Proteins 0.000 claims description 2
- 101150026369 MT-ND6 gene Proteins 0.000 claims description 2
- 102000013379 Mitochondrial Proton-Translocating ATPases Human genes 0.000 claims description 2
- 108010026155 Mitochondrial Proton-Translocating ATPases Proteins 0.000 claims description 2
- 101000954570 Mus musculus V-type proton ATPase 16 kDa proteolipid subunit c Proteins 0.000 claims description 2
- 102100028386 NADH-ubiquinone oxidoreductase chain 6 Human genes 0.000 claims description 2
- 101150090932 ND6 gene Proteins 0.000 claims description 2
- 102000005877 Peptide Initiation Factors Human genes 0.000 claims description 2
- 108010044843 Peptide Initiation Factors Proteins 0.000 claims description 2
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 claims description 2
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 claims description 2
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 claims description 2
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 claims description 2
- 102100031712 Splicing factor 3A subunit 2 Human genes 0.000 claims description 2
- 102000004399 TNF receptor-associated factor 3 Human genes 0.000 claims description 2
- 108090000922 TNF receptor-associated factor 3 Proteins 0.000 claims description 2
- 102100035147 Transcriptional enhancer factor TEF-5 Human genes 0.000 claims description 2
- 102100029887 Translationally-controlled tumor protein Human genes 0.000 claims description 2
- 102000004243 Tubulin Human genes 0.000 claims description 2
- 108090000704 Tubulin Proteins 0.000 claims description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 108010022838 lamin B receptor Proteins 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 210000000056 organ Anatomy 0.000 claims description 2
- 102000027426 receptor tyrosine kinases Human genes 0.000 claims description 2
- 108091008598 receptor tyrosine kinases Proteins 0.000 claims description 2
- 229930002330 retinoic acid Natural products 0.000 claims description 2
- 210000001324 spliceosome Anatomy 0.000 claims description 2
- 238000010361 transduction Methods 0.000 claims description 2
- 230000026683 transduction Effects 0.000 claims description 2
- 230000014621 translational initiation Effects 0.000 claims description 2
- 229960001727 tretinoin Drugs 0.000 claims description 2
- 101001109052 Homo sapiens NADH-ubiquinone oxidoreductase chain 4 Proteins 0.000 claims 1
- 101000628647 Homo sapiens Serine/threonine-protein kinase 24 Proteins 0.000 claims 1
- 102100025315 Mannosyl-oligosaccharide glucosidase Human genes 0.000 claims 1
- 102100021506 NADH-ubiquinone oxidoreductase chain 4 Human genes 0.000 claims 1
- 102000007568 Proto-Oncogene Proteins c-fos Human genes 0.000 claims 1
- 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 claims 1
- 102100026764 Serine/threonine-protein kinase 24 Human genes 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 108010050669 glucosidase I Proteins 0.000 claims 1
- 102100023793 tRNA modification GTPase GTPBP3, mitochondrial Human genes 0.000 claims 1
- 231100000440 toxicity profile Toxicity 0.000 abstract description 9
- 238000011156 evaluation Methods 0.000 abstract description 7
- 238000010276 construction Methods 0.000 abstract description 6
- 231100000721 toxic potential Toxicity 0.000 abstract description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 3
- 239000008194 pharmaceutical composition Substances 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 37
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 37
- 230000000875 corresponding effect Effects 0.000 description 36
- 230000030833 cell death Effects 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 239000013615 primer Substances 0.000 description 8
- 231100000167 toxic agent Toxicity 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 239000002773 nucleotide Substances 0.000 description 7
- 125000003729 nucleotide group Chemical group 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000001574 biopsy Methods 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000035755 proliferation Effects 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 230000019491 signal transduction Effects 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 5
- 230000036425 denaturation Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 230000037353 metabolic pathway Effects 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 230000001640 apoptogenic effect Effects 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 230000002596 correlated effect Effects 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 230000006882 induction of apoptosis Effects 0.000 description 4
- 239000003999 initiator Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 210000002569 neuron Anatomy 0.000 description 4
- 230000001575 pathological effect Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000011727 Caspases Human genes 0.000 description 3
- 108010076667 Caspases Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 102100034343 Integrase Human genes 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000004075 alteration Effects 0.000 description 3
- 238000003782 apoptosis assay Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000001738 genotoxic effect Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 210000005260 human cell Anatomy 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 230000005522 programmed cell death Effects 0.000 description 3
- AQHHHDLHHXJYJD-UHFFFAOYSA-N propranolol Chemical class C1=CC=C2C(OCC(O)CNC(C)C)=CC=CC2=C1 AQHHHDLHHXJYJD-UHFFFAOYSA-N 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000003196 serial analysis of gene expression Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 102000051485 Bcl-2 family Human genes 0.000 description 2
- 108700038897 Bcl-2 family Proteins 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 108020004518 RNA Probes Proteins 0.000 description 2
- 239000003391 RNA probe Substances 0.000 description 2
- 108700025701 Retinoblastoma Genes Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 206010070863 Toxicity to various agents Diseases 0.000 description 2
- 108010078814 Tumor Suppressor Protein p53 Proteins 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000036952 cancer formation Effects 0.000 description 2
- 231100000504 carcinogenesis Toxicity 0.000 description 2
- 230000025084 cell cycle arrest Effects 0.000 description 2
- 230000033077 cellular process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 description 2
- 229960001117 clenbuterol Drugs 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000009509 drug development Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 108010052621 fas Receptor Proteins 0.000 description 2
- 102000018823 fas Receptor Human genes 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000002547 new drug Substances 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 238000011330 nucleic acid test Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- -1 oligonucleotides Chemical class 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- AQHHHDLHHXJYJD-AWEZNQCLSA-N (2s)-1-naphthalen-1-yloxy-3-(propan-2-ylamino)propan-2-ol Chemical compound C1=CC=C2C(OC[C@@H](O)CNC(C)C)=CC=CC2=C1 AQHHHDLHHXJYJD-AWEZNQCLSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 102100034682 26S proteasome regulatory subunit 7 Human genes 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108010029607 4-nitrophenyl-alpha-glucosidase Proteins 0.000 description 1
- 102100022900 Actin, cytoplasmic 1 Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 238000010953 Ames test Methods 0.000 description 1
- 231100000039 Ames test Toxicity 0.000 description 1
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 102100033040 Carbonic anhydrase 12 Human genes 0.000 description 1
- 102100033029 Carbonic anhydrase-related protein 11 Human genes 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 description 1
- 108010036949 Cyclosporine Proteins 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 102000010170 Death domains Human genes 0.000 description 1
- 108050001718 Death domains Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 description 1
- 102100038980 Exosome complex component CSL4 Human genes 0.000 description 1
- 101150064015 FAS gene Proteins 0.000 description 1
- 230000010190 G1 phase Effects 0.000 description 1
- 101150031823 HSP70 gene Proteins 0.000 description 1
- 206010019851 Hepatotoxicity Diseases 0.000 description 1
- 108091027305 Heteroduplex Proteins 0.000 description 1
- 101000867855 Homo sapiens Carbonic anhydrase 12 Proteins 0.000 description 1
- 101000867841 Homo sapiens Carbonic anhydrase-related protein 11 Proteins 0.000 description 1
- 101001075218 Homo sapiens Gastrokine-1 Proteins 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 206010043275 Teratogenicity Diseases 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108700014807 Translationally-Controlled 1 Tumor Proteins 0.000 description 1
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 1
- 101800001476 Viral genome-linked protein Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 230000003217 anti-cancerogenic effect Effects 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 230000005775 apoptotic pathway Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012148 binding buffer Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000013043 cell viability test Methods 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 230000004637 cellular stress Effects 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229960001265 ciclosporin Drugs 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229930182912 cyclosporin Natural products 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- DCOPUUMXTXDBNB-UHFFFAOYSA-N diclofenac Chemical compound OC(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl DCOPUUMXTXDBNB-UHFFFAOYSA-N 0.000 description 1
- 229960001259 diclofenac Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 101150052825 dnaK gene Proteins 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 201000010536 head and neck cancer Diseases 0.000 description 1
- 208000014829 head and neck neoplasm Diseases 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000007686 hepatotoxicity Effects 0.000 description 1
- 231100000304 hepatotoxicity Toxicity 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 108091008147 housekeeping proteins Proteins 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 230000006680 metabolic alteration Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 231100000243 mutagenic effect Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 231100001160 nonlethal Toxicity 0.000 description 1
- 238000003499 nucleic acid array Methods 0.000 description 1
- 101800000605 p13 Proteins 0.000 description 1
- 108700025694 p53 Genes Proteins 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 230000000861 pro-apoptotic effect Effects 0.000 description 1
- 229960003712 propranolol Drugs 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 231100000004 severe toxicity Toxicity 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000004960 subcellular localization Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 231100000378 teratogenic Toxicity 0.000 description 1
- 230000003390 teratogenic effect Effects 0.000 description 1
- 231100000211 teratogenicity Toxicity 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 230000030968 tissue homeostasis Effects 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000008207 working material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9911405A FR2798392B1 (fr) | 1999-09-13 | 1999-09-13 | Marqueurs genetiques de la toxicite, preparation et utilisations |
| FR99/11405 | 1999-09-13 | ||
| PCT/FR2000/002503 WO2001020029A2 (fr) | 1999-09-13 | 2000-09-12 | Marquers genetiques de la toxicite, preparation et utilisations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003509066A true JP2003509066A (ja) | 2003-03-11 |
| JP2003509066A5 JP2003509066A5 (https=) | 2007-11-08 |
Family
ID=9549766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001523800A Pending JP2003509066A (ja) | 1999-09-13 | 2000-09-12 | 遺伝学的毒性マーカー、その調製及び使用 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6509153B1 (https=) |
| EP (1) | EP1220950B9 (https=) |
| JP (1) | JP2003509066A (https=) |
| AT (1) | ATE263255T1 (https=) |
| AU (1) | AU783310B2 (https=) |
| CA (1) | CA2380383A1 (https=) |
| DE (1) | DE60009530T2 (https=) |
| DK (1) | DK1220950T3 (https=) |
| ES (1) | ES2216958T3 (https=) |
| FR (1) | FR2798392B1 (https=) |
| IL (1) | IL148615A0 (https=) |
| PT (1) | PT1220950E (https=) |
| WO (1) | WO2001020029A2 (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010507809A (ja) * | 2006-10-23 | 2010-03-11 | ザ ユーエービー リサーチ ファウンデーション | がん感受性についてのバイオマーカー及びその用途 |
| JP2013534820A (ja) * | 2010-06-24 | 2013-09-09 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | 遺伝毒性化合物を同定し、特徴付けるための遺伝子発現分析 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6881571B1 (en) * | 1998-03-11 | 2005-04-19 | Exonhit Therapeutics S.A. | Qualitative differential screening |
| FR2798673B1 (fr) * | 1999-09-16 | 2004-05-28 | Exonhit Therapeutics Sa | Methodes et compositions pour la detection d'evenements pathologiques |
| US7361488B2 (en) * | 2000-02-07 | 2008-04-22 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| US20050112573A1 (en) * | 2001-08-24 | 2005-05-26 | Hitoshi Iwahashi | Method of detecting toxic substance |
| GB0410105D0 (en) * | 2004-05-06 | 2004-06-09 | Amersham Biosciences Uk Ltd | Method for characterising compounds |
| US20080194417A1 (en) * | 2005-03-09 | 2008-08-14 | Luca Barella | Method for Measuring and Comparing the Activity of Biologically Active Compounds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010529A1 (en) * | 1997-08-22 | 1999-03-04 | Zeneca Limited | Methods for analyzing ltc4 synthase polymorphisms and diagnostic use |
| WO1999014356A2 (en) * | 1997-09-17 | 1999-03-25 | The Johns Hopkins University | P53-induced apoptosis |
| JP2002505887A (ja) * | 1998-03-11 | 2002-02-26 | エグゾニ・テラピューティック・ソシエテ・アノニム | 示差スクリーニング |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4888278A (en) * | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
| US5985549A (en) * | 1985-10-22 | 1999-11-16 | University Of Massachusetts | Non-isotopic in-situ hybridization method for detection of nucleic acids |
| US5057410A (en) * | 1988-08-05 | 1991-10-15 | Cetus Corporation | Chimeric messenger RNA detection methods |
| US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US6309822B1 (en) * | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US5674682A (en) * | 1992-10-29 | 1997-10-07 | Thomas Jefferson University | Nucleic acid primers for detecting micrometastasis of prostate cancer |
| US5521297A (en) * | 1993-06-04 | 1996-05-28 | Salk Institute Biotechnology/Industrial Associates | Nucleic acids encoding human metabotropic glutamate receptors |
| US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| US5529925A (en) * | 1993-12-03 | 1996-06-25 | St. Jude Children's Research Hospital | Nucleic acid sequences and fusion proteins present in human t(2;5) lymphoma |
| US5484702A (en) * | 1994-01-27 | 1996-01-16 | Research Foundation Of The State University Of New York At Buffalo | Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation with preselected clones |
| US5679541A (en) * | 1994-02-14 | 1997-10-21 | California Institute Of Technology | Programmed cell death antagonist protein |
| PH31414A (en) * | 1994-02-24 | 1998-10-29 | Boehringer Ingelheim Int | Method of diagnosing cancer precancerous state, orsusceptibility to other forms of diseases by anal ysis of irf-1 specific rna in biopsy samples. |
| US5767258A (en) * | 1995-05-12 | 1998-06-16 | The Johns Hopkins University School Of Medicine | Cell cycle regulatory gene |
| WO1997013877A1 (en) * | 1995-10-12 | 1997-04-17 | Lynx Therapeutics, Inc. | Measurement of gene expression profiles in toxicity determination |
| US5922535A (en) * | 1995-11-07 | 1999-07-13 | Huo; Li | Identifying sequence differences in nucleic acid populations |
| US6881571B1 (en) * | 1998-03-11 | 2005-04-19 | Exonhit Therapeutics S.A. | Qualitative differential screening |
| FR2755984B1 (fr) * | 1996-11-15 | 2002-12-27 | Bourachot Philippe | Borne escamotable |
| US6060240A (en) * | 1996-12-13 | 2000-05-09 | Arcaris, Inc. | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
| CA2225187A1 (en) * | 1997-07-14 | 1999-01-14 | Universite D'ottawa/ University Of Ottawa | Xaf genes and polypeptides: methods and reagents for modulating apoptosis |
| US6849400B1 (en) * | 1997-07-23 | 2005-02-01 | Gen-Probe Incorporated | Methods for detecting and measuring spliced nucleic acids |
| EP1548123A1 (en) * | 1998-02-23 | 2005-06-29 | Phylonix Pharmaceuticals Inc. | Methods of screening agents for activity using teleosts |
| JPH11262621A (ja) * | 1998-03-17 | 1999-09-28 | Ebara Corp | 除湿空調装置 |
| US6168920B1 (en) * | 1998-08-10 | 2001-01-02 | Incyte Genomics, Inc. | Extracellular adhesive proteins |
| US6160105A (en) * | 1998-10-13 | 2000-12-12 | Incyte Pharmaceuticals, Inc. | Monitoring toxicological responses |
| CA2340589A1 (en) * | 1998-08-28 | 2000-03-09 | Incyte Pharmaceuticals, Inc. | Toxicological response markers |
| FR2798673B1 (fr) * | 1999-09-16 | 2004-05-28 | Exonhit Therapeutics Sa | Methodes et compositions pour la detection d'evenements pathologiques |
| CA2450577A1 (en) * | 2001-06-21 | 2003-01-03 | Diversa Corporation | Methods for the manufacture of pure single enantiomer compounds and for selecting enantioselective enzymes |
-
1999
- 1999-09-13 FR FR9911405A patent/FR2798392B1/fr not_active Expired - Fee Related
- 1999-12-08 US US09/456,370 patent/US6509153B1/en not_active Expired - Fee Related
-
2000
- 2000-09-12 CA CA002380383A patent/CA2380383A1/fr not_active Abandoned
- 2000-09-12 EP EP00962602A patent/EP1220950B9/fr not_active Expired - Lifetime
- 2000-09-12 AU AU74269/00A patent/AU783310B2/en not_active Ceased
- 2000-09-12 DK DK00962602T patent/DK1220950T3/da active
- 2000-09-12 JP JP2001523800A patent/JP2003509066A/ja active Pending
- 2000-09-12 WO PCT/FR2000/002503 patent/WO2001020029A2/fr not_active Ceased
- 2000-09-12 ES ES00962602T patent/ES2216958T3/es not_active Expired - Lifetime
- 2000-09-12 DE DE60009530T patent/DE60009530T2/de not_active Expired - Lifetime
- 2000-09-12 PT PT00962602T patent/PT1220950E/pt unknown
- 2000-09-12 IL IL14861500A patent/IL148615A0/xx unknown
- 2000-09-12 AT AT00962602T patent/ATE263255T1/de not_active IP Right Cessation
-
2001
- 2001-03-22 US US10/070,676 patent/US20030059788A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010529A1 (en) * | 1997-08-22 | 1999-03-04 | Zeneca Limited | Methods for analyzing ltc4 synthase polymorphisms and diagnostic use |
| WO1999014356A2 (en) * | 1997-09-17 | 1999-03-25 | The Johns Hopkins University | P53-induced apoptosis |
| JP2002505887A (ja) * | 1998-03-11 | 2002-02-26 | エグゾニ・テラピューティック・ソシエテ・アノニム | 示差スクリーニング |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010507809A (ja) * | 2006-10-23 | 2010-03-11 | ザ ユーエービー リサーチ ファウンデーション | がん感受性についてのバイオマーカー及びその用途 |
| JP2013534820A (ja) * | 2010-06-24 | 2013-09-09 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | 遺伝毒性化合物を同定し、特徴付けるための遺伝子発現分析 |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2798392A1 (fr) | 2001-03-16 |
| CA2380383A1 (fr) | 2001-03-22 |
| US20030059788A1 (en) | 2003-03-27 |
| EP1220950B9 (fr) | 2004-11-03 |
| PT1220950E (pt) | 2004-07-30 |
| ATE263255T1 (de) | 2004-04-15 |
| WO2001020029A3 (fr) | 2001-11-29 |
| AU7426900A (en) | 2001-04-17 |
| DE60009530D1 (de) | 2004-05-06 |
| US6509153B1 (en) | 2003-01-21 |
| AU783310B2 (en) | 2005-10-13 |
| EP1220950B1 (fr) | 2004-03-31 |
| DK1220950T3 (da) | 2004-08-02 |
| FR2798392B1 (fr) | 2005-07-15 |
| EP1220950A2 (fr) | 2002-07-10 |
| ES2216958T3 (es) | 2004-11-01 |
| IL148615A0 (en) | 2002-09-12 |
| DE60009530T2 (de) | 2005-02-03 |
| WO2001020029A2 (fr) | 2001-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0743989B1 (en) | Methed of identifying differentially expressed genes | |
| US6251590B1 (en) | Differential Qualitative screening | |
| Burmistrova et al. | MicroRNA in schizophrenia: genetic and expression analysis of miR-130b (22q11) | |
| US8003375B2 (en) | Qualitative differential screening | |
| AU774263B2 (en) | Methods and compositions for detecting pathological events | |
| JP2019531741A (ja) | 胃癌の生物学的特性に基づく群区分および予後予測システム | |
| US20050003410A1 (en) | Allele-specific expression patterns | |
| US20100144555A1 (en) | Qualitative differential screening | |
| Dwivedi et al. | Application of single-cell omics in breast cancer | |
| Darnell | Developing global insight into RNA regulation | |
| WO2001032927A2 (en) | Tissue specific genes of diagnostic import | |
| JP2003509066A (ja) | 遺伝学的毒性マーカー、その調製及び使用 | |
| US20060024705A1 (en) | Molecular analysis of hair follicles for disease | |
| CA2803677A1 (en) | Gene expression analyses for characterizing and identifying genotoxic compounds | |
| Kharlap et al. | Atrial appendage transcriptional profile in patients with atrial fibrillation with structural heart diseases | |
| CN113637758B (zh) | miR-19b/miR-26a作为标志物在制备恶性间皮瘤检测试剂盒中的应用 | |
| EP2057288A2 (en) | Method of prognosis | |
| Orlando | Functional Annotation of Human Long Non-Coding RNAs via Molecular Phenotyping | |
| WO2004007768A1 (en) | Method to analyze polymeric nucleic acid sequence variations | |
| JPWO2000065050A1 (ja) | 花粉症関連遺伝子、７９５ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070907 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070907 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100629 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20101221 |