JP2003339395A5 - - Google Patents

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Publication number
JP2003339395A5
JP2003339395A5 JP2002152245A JP2002152245A JP2003339395A5 JP 2003339395 A5 JP2003339395 A5 JP 2003339395A5 JP 2002152245 A JP2002152245 A JP 2002152245A JP 2002152245 A JP2002152245 A JP 2002152245A JP 2003339395 A5 JP2003339395 A5 JP 2003339395A5
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JP
Japan
Prior art keywords
cell
protein synthesis
free protein
germ
embryo
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Pending
Application number
JP2002152245A
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English (en)
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JP2003339395A (ja
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Priority to JP2002152245A priority Critical patent/JP2003339395A/ja
Priority claimed from JP2002152245A external-priority patent/JP2003339395A/ja
Publication of JP2003339395A publication Critical patent/JP2003339395A/ja
Publication of JP2003339395A5 publication Critical patent/JP2003339395A5/ja
Pending legal-status Critical Current

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Claims (4)

  1. 衝撃式粉砕機を用いて、衝撃速度２５〜７０ｍ／ｓで胚芽を植物種子から分離することを含む、無細胞タンパク質合成用胚芽抽出物の製造方法。
  2. 胚芽が、コムギ、オオムギ又はイネの胚芽である請求項１記載の方法。
  3. 請求項１又は２に記載の方法で製造された胚芽抽出物を含有する無細胞タンパク質合成用溶液。
  4. ＡＴＰ、ＧＴＰ、クレアチンリン酸、クレアチンキナーゼ、Ｌ型アミノ酸、カリウムイオン及びマグネシウムイオンを含む請求項３記載の無細胞タンパク質合成用溶液。
JP2002152245A 2002-05-27 2002-05-27 無細胞タンパク質合成用胚芽の選別方法および無細胞タンパク質合成用胚芽抽出物の製造方法 Pending JP2003339395A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2002152245A JP2003339395A (ja) 2002-05-27 2002-05-27 無細胞タンパク質合成用胚芽の選別方法および無細胞タンパク質合成用胚芽抽出物の製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002152245A JP2003339395A (ja) 2002-05-27 2002-05-27 無細胞タンパク質合成用胚芽の選別方法および無細胞タンパク質合成用胚芽抽出物の製造方法

Publications (2)

Publication Number Publication Date
JP2003339395A JP2003339395A (ja) 2003-12-02
JP2003339395A5 true JP2003339395A5 (ja) 2005-10-06

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ID=29769620

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002152245A Pending JP2003339395A (ja) 2002-05-27 2002-05-27 無細胞タンパク質合成用胚芽の選別方法および無細胞タンパク質合成用胚芽抽出物の製造方法

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JP (1) JP2003339395A (ja)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US8044260B2 (en) 2007-03-09 2011-10-25 Monsanto Technology Llc Method of meristem excision and transformation

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