JP2003320016A - Living tissue filling material, living tissue filling body, and method and device of manufacturing living tissue filling body - Google Patents
Living tissue filling material, living tissue filling body, and method and device of manufacturing living tissue filling bodyInfo
- Publication number
- JP2003320016A JP2003320016A JP2002129263A JP2002129263A JP2003320016A JP 2003320016 A JP2003320016 A JP 2003320016A JP 2002129263 A JP2002129263 A JP 2002129263A JP 2002129263 A JP2002129263 A JP 2002129263A JP 2003320016 A JP2003320016 A JP 2003320016A
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- Japan
- Prior art keywords
- living tissue
- tissue filling
- filling material
- cells
- bone
- Prior art date
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、生体組織欠損部を
再生する際に使用される生体組織補填材、生体組織補填
体、生体組織補填体の製造方法および生体組織補填体の
製造装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a living tissue filling material, a living tissue filling body, a method for manufacturing a living tissue filling body, and a living tissue filling body manufacturing apparatus used for regenerating a living tissue defective portion.
【０００２】[0002]
【従来の技術】近年、骨腫瘍摘出や外傷等により生じた
骨欠損部等の生体組織欠損部に骨補填材等の生体組織補
填材を補填することにより、骨等の生体組織を再生させ
て骨欠損部等を修復することが可能になってきている。
骨補填材としては、ハイドロキシアパタイト（ＨＡＰ）
やリン酸三カルシウム（ＴＣＰ）が知られているが、体
内に異物を残さないとする考え方から、例えば、β−Ｔ
ＣＰのようなリン酸カルシウム多孔体からなる足場材が
使用される。β−ＴＣＰを骨欠損部の骨細胞に接触させ
ておくと、破骨細胞がβ−ＴＣＰを食べ、骨芽細胞が新
しい骨を形成する、いわゆるリモデリングが行われる。
すなわち、骨欠損部に補填された骨補填材は、経時的に
自家骨に置換されていくことになる。2. Description of the Related Art In recent years, a living tissue such as a bone has been regenerated by filling a living tissue defect such as a bone defect caused by excision of a bone tumor or trauma with a living tissue repair material such as a bone filling material. It has become possible to repair bone defects and the like.
Hydroxyapatite (HAP) as bone filling material
And tricalcium phosphate (TCP) are known, but from the idea that no foreign matter remains in the body, for example, β-T
A scaffolding material made of porous calcium phosphate such as CP is used. When β-TCP is brought into contact with bone cells in a bone defect, osteoclasts eat β-TCP and osteoblasts form new bone, so-called remodeling is performed.
That is, the bone filling material filled in the bone defect portion is replaced with autogenous bone over time.
【０００３】ところで、術後の骨欠損部の修復速度を高
めるために、骨補填材をそのまま用いるのではなく、患
者から採取した細胞を骨補填材に付与して骨補填体と
し、このような骨補填体を骨欠損部に補填することが行
われている。By the way, in order to increase the speed of repair of a postoperative bone defect, the bone prosthetic material is not used as it is, but cells collected from a patient are added to the bone prosthetic material to form a bone prosthetic material. The bone filling body is filled with a bone defect portion.
【０００４】[0004]
【発明が解決しようとする課題】この場合、患者から骨
髄液を抽出し、骨補填材をこの骨髄液に浸した後に、骨
欠損部に補填することになる。しかしながら、単に骨補
填材を骨髄液に浸すだけでは、手術中という限られた時
間の中で骨補填材に十分に細胞を付与することができな
いという問題があった。In this case, bone marrow fluid is extracted from the patient, the bone filling material is immersed in this bone marrow fluid, and then the bone defect is filled. However, there is a problem that simply immersing the bone prosthetic material in the bone marrow fluid cannot sufficiently add cells to the bone prosthetic material within a limited time during surgery.
【０００５】したがって、本発明は、限られた時間の中
でも十分に細胞を付与できる生体組織補填材、生体組織
補填体、生体組織補填体の製造方法および生体組織補填
体の製造装置の提供を目的とする。Therefore, the object of the present invention is to provide a living tissue filling material, a living tissue filling body, a method for producing a living tissue filling body, and an apparatus for producing a living tissue filling body, which can provide sufficient cells even in a limited time. And
【０００６】[0006]
【課題を解決するための手段】上記目的を達成するため
に、請求項１に係る発明は、細胞が付与された後に生体
組織欠損部に補填される生体組織補填材において、少な
くともその一部が負に帯電していることを特徴としてい
る。In order to achieve the above-mentioned object, the invention according to claim 1 is a living tissue filling material to be filled in a living tissue defective portion after cells are added, at least part of which is It is characterized by being negatively charged.
【０００７】このように負に帯電しているため、負に帯
電した部分に結合しやすい細胞が容易に付着する。した
がって、限られた時間の中でも十分に細胞が付与される
ことになり、その結果、術後の生体組織欠損部の修復速
度を高めることができる。Since the cells are negatively charged in this manner, cells that are likely to bind to the negatively charged portions easily attach to them. Therefore, the cells are sufficiently added even in a limited time, and as a result, the rate of repair of the biological tissue defect portion after surgery can be increased.
【０００８】請求項２に係る発明は、請求項１に係る発
明に関し、前記生体組織欠損部は骨欠損部であることを
特徴としている。The invention according to claim 2 relates to the invention according to claim 1, characterized in that the living tissue defective portion is a bone defective portion.
【０００９】請求項３に係る発明は、生体組織補填材に
細胞が付与されて構成され、生体組織欠損部に補填され
る生体組織補填体において、前記生体組織補填材の少な
くとも一部が負に帯電していることを特徴としている。According to a third aspect of the present invention, in a living tissue filling body configured by adding cells to a living tissue filling material and filling a living tissue defective portion, at least part of the living tissue filling material is negative. It is characterized by being charged.
【００１０】このように生体組織補填材が負に帯電して
いるため、負に帯電した部分に結合しやすい細胞が容易
に付着する。したがって、限られた時間の中でも十分に
生体組織補填材に細胞が付与されることになり、その結
果、術後の生体組織欠損部の修復速度を高めることがで
きる。Since the biological tissue filling material is negatively charged as described above, cells that are easily bonded to the negatively charged portion easily attach to the negatively charged portion. Therefore, the cells can be sufficiently added to the biological tissue filling material even within a limited time, and as a result, the rate of repair of the biological tissue defective portion after surgery can be increased.
【００１１】請求項４に係る発明は、請求項３に係る発
明に関し、前記生体組織欠損部は骨欠損部であることを
特徴としている。The invention according to claim 4 relates to the invention according to claim 3, characterized in that the living tissue defective portion is a bone defective portion.
【００１２】請求項５に係る発明は、細胞を多孔質の生
体組織補填材に浸透させることにより、生体組織欠損部
に補填される生体組織補填体を形成する生体組織補填体
の製造方法であって、前記細胞を容器内で電気泳動によ
り移動させ、該移動により前記生体組織補填材に前記細
胞を浸透させることを特徴としている。[0012] The invention according to claim 5 is a method for producing a biological tissue filling body, which comprises forming a living tissue filling body to be filled in a living tissue defective portion by permeating cells into a porous living tissue filling material. Then, the cells are electrophoretically moved in the container, and the movement causes the cells to permeate the biological tissue filling material.
【００１３】このように、細胞を容器内で電気泳動によ
り移動させ、該移動により生体組織補填材に細胞を浸透
させるため、生体組織補填材の内部に細胞が強制的に浸
透させられる。したがって、限られた時間の中でも、生
体組織補填材に十分に細胞が付与された生体組織補填体
を得ることができることになり、その結果、術後の生体
組織欠損部の修復速度を高めることができる。As described above, since the cells are electrophoretically moved in the container and the migration causes the cells to permeate the biological tissue filling material, the cells are forcibly permeated into the biological tissue filling material. Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００１４】請求項６に係る発明は、請求項５に係る発
明に関し、前記電気泳動用の負極の近傍に前記生体組織
補填材を配置することを特徴としている。The invention according to claim 6 relates to the invention according to claim 5, characterized in that the biological tissue filling material is arranged in the vicinity of the negative electrode for electrophoresis.
【００１５】このように、電気泳動用の負極の近傍に生
体組織補填材を配置するため、電気泳動で負に帯電した
部分に結合しやすい細胞が生体組織補填材に集まって浸
透させられる。したがって、限られた時間の中でも、生
体組織補填材に一層多くの細胞が付与された生体組織補
填体を得ることができることになり、その結果、術後の
生体組織欠損部の修復速度をさらに高めることができ
る。Since the biological tissue filling material is arranged in the vicinity of the negative electrode for electrophoresis as described above, cells that are likely to bind to the negatively charged portion by electrophoresis gather and permeate into the biological tissue filling material. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to.
【００１６】請求項７に係る発明は、請求項５に係る発
明に関し、前記生体組織補填材を前記電気泳動用の負極
とすることを特徴としている。The invention according to claim 7 relates to the invention according to claim 5, characterized in that the living tissue filling material is used as the negative electrode for electrophoresis.
【００１７】このように、電気泳動用の負極として生体
組織補填材を配置するため、電気泳動で負に帯電した部
分に結合しやすい細胞が生体組織補填材に集まって浸透
させられる。したがって、限られた時間の中でも、生体
組織補填材に一層多くの細胞が付与された生体組織補填
体を得ることができることになり、その結果、術後の生
体組織欠損部の修復速度をさらに高めることができる。Since the biological tissue filling material is arranged as the negative electrode for electrophoresis in this manner, cells which are easily bound to the negatively charged portion by electrophoresis are collected in the biological tissue filling material and permeated. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to.
【００１８】請求項８に係る発明は、細胞を多孔質の生
体組織補填材に浸透させることにより、生体組織欠損部
に補填される生体組織補填体を形成する生体組織補填体
の製造方法であって、前記生体組織補填材の内側に前記
細胞が注入される注入空間を形成し、前記生体組織補填
材の外面に負極を配置するとともに前記注入空間に注入
された前記細胞に正極を配置して電圧を印加することを
特徴としている。The invention according to claim 8 is a method for producing a biological tissue filling body, which comprises forming a living tissue filling body to be filled in a living tissue defective portion by infiltrating cells into a porous living tissue filling material. Then, an injection space into which the cells are injected is formed inside the living tissue filling material, a negative electrode is arranged on the outer surface of the living tissue filling material, and a positive electrode is arranged on the cells injected into the filling space. The feature is that a voltage is applied.
【００１９】これにより、生体組織補填材の外面に配置
された負極と生体組織補填材の注入空間に注入された細
胞に配置された正極とに電圧を印加すると、負に帯電し
た部分に結合しやすい細胞が生体組織補填材を内側から
外側に向けて移動させられて生体組織補填材に浸透させ
られる。したがって、限られた時間の中でも、生体組織
補填材に細胞が十分に付与された生体組織補填体を得る
ことができることになり、その結果、術後の生体組織欠
損部の修復速度を高めることができる。As a result, when a voltage is applied to the negative electrode arranged on the outer surface of the biological tissue filling material and the positive electrode arranged on the cells injected into the injection space of the biological tissue filling material, they are bonded to the negatively charged portion. The easy cells are moved from the inside of the biological tissue filling material toward the outside to penetrate the living tissue filling material. Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００２０】請求項９に係る発明は、細胞を生体組織補
填材に付与することにより、生体組織欠損部に補填され
る生体組織補填体を形成する生体組織補填体の製造方法
であって、前記生体組織補填材を負に帯電させることを
特徴としている。The invention according to claim 9 is a method for producing a biological tissue filling body, which comprises forming a living tissue filling body to be filled in a living tissue defective portion by applying cells to the living tissue filling material, The feature is that the biological tissue filling material is negatively charged.
【００２１】このように、生体組織補填材を負に帯電さ
せるため、生体組織補填材に塗布あるいは散布等され
た、負に帯電した部分に結合しやすい細胞が生体組織補
填材に容易に付着する。したがって、限られた時間の中
でも十分に生体組織補填材に細胞が付与されることにな
り、その結果、術後の生体組織欠損部の修復速度を高め
ることができる。As described above, since the living tissue filling material is negatively charged, the cells which are applied or sprayed on the living tissue filling material and are easily bonded to the negatively charged portion easily attach to the living tissue filling material. . Therefore, the cells can be sufficiently added to the biological tissue filling material even within a limited time, and as a result, the rate of repair of the biological tissue defective portion after surgery can be increased.
【００２２】請求項１０に係る発明は、細胞を生体組織
補填材に付与することにより、生体組織欠損部に補填さ
れる生体組織補填体を形成する生体組織補填体の製造装
置であって、前記生体組織補填材を負に帯電させること
を特徴としている。A tenth aspect of the present invention is an apparatus for producing a biological tissue filling body, which forms a living tissue filling body to be filled in a living tissue defective portion by applying cells to the living tissue filling material, The feature is that the biological tissue filling material is negatively charged.
【００２３】請求項１１に係る発明は、細胞を多孔質の
生体組織補填材に浸透させることにより、生体組織欠損
部に補填される生体組織補填体を形成する生体組織補填
体の製造装置であって、前記細胞が注入されるとともに
通路を有する容器と、該容器の前記通路内で電気泳動に
より前記細胞を移動させる電気泳動部と、装填された前
記生体組織補填材を前記通路に配置する装填部とを有す
ることを特徴としている。The invention according to claim 11 is an apparatus for producing a biological tissue filling body, which forms a biological tissue filling body to be filled in a living tissue defective portion by permeating cells into a porous living tissue filling material. A container into which the cells are injected and which has a passage, an electrophoretic section for moving the cells by electrophoresis in the passage of the container, and a loading for disposing the loaded biological tissue filling material in the passage. It is characterized by having a part.
【００２４】これにより、装填部に生体組織補填材を装
填すると、生体組織補填材が自動的に電気泳動で細胞を
移動させる通路に配置される。よって、通路で細胞を電
気泳動により移動させると生体組織補填材に細胞が強制
的に浸透させられる。したがって、限られた時間の中で
も、生体組織補填材に十分に細胞が付与された生体組織
補填体を得ることができることになり、その結果、術後
の生体組織欠損部の修復速度を高めることができる。As a result, when the biological tissue filling material is loaded in the loading section, the biological tissue filling material is automatically arranged in the passage for moving cells by electrophoresis. Therefore, when the cells are electrophoretically moved in the passage, the cells are forcibly permeated into the biological tissue filling material. Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００２５】請求項１２に係る発明は、細胞を多孔質の
生体組織補填材に浸透させることにより、生体組織欠損
部に補填される生体組織補填体を形成する生体組織補填
体の製造装置であって、前記細胞が注入される容器と、
該容器に注入された前記細胞を電気泳動させる正極およ
び負極と、装填された前記生体組織補填材を前記負極に
接触させる装填部とを有することを特徴としている。The invention according to claim 12 is the apparatus for producing a living tissue filling body, which forms a living tissue filling body to be filled in a living tissue defective portion by permeating cells into a porous living tissue filling material. And a container into which the cells are injected,
It is characterized by having a positive electrode and a negative electrode for electrophoresing the cells injected into the container, and a loading part for bringing the loaded biological tissue filling material into contact with the negative electrode.
【００２６】これにより、装填部に生体組織補填材を装
填すると、生体組織補填材が自動的に電気泳動用の負極
に接触させられ、電気泳動で負に帯電した部分に結合し
やすい細胞が生体組織補填材に集まって浸透させられ
る。したがって、限られた時間の中でも、生体組織補填
材に一層多くの細胞が付与された生体組織補填体を得る
ことができることになり、その結果、術後の生体組織欠
損部の修復速度をさらに高めることができる。しかも、
生体組織補填材が装填部への装填で自動的に電気泳動用
の負極に接触させられるため、生体組織補填材をセット
する作業が容易となる。As a result, when the living tissue filling material is loaded in the loading portion, the living tissue filling material is automatically brought into contact with the negative electrode for electrophoresis, and cells that easily bind to the negatively charged portion by electrophoresis are formed in the living body. Collected in tissue filling material and permeated. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to. Moreover,
Since the biological tissue filling material is automatically brought into contact with the negative electrode for electrophoresis when loaded into the loading portion, the work of setting the biological tissue filling material becomes easy.
【００２７】[0027]
【発明の実施の形態】本発明の第１実施形態を図１〜図
３を参照して以下に説明する。ここで、第１実施形態に
おいて製造される生体組織補填材としての骨補填体のベ
ースとなる骨補填材１１として、例えば、特開平５−２
３７１７８号公報に開示されている方法により製造され
たβ−ＴＣＰ（β−リン酸三カルシウム）からなる直方
体状の多孔体が使用される。β−ＴＣＰのようなセラミ
ックスの多孔体は、焼結製造時に表面に電圧を印加する
ことで、その後は電圧を印加しなくても常に全面的に負
に帯電するものとなる（図１はこのように全面的に負に
帯電しているイメージを示している）。DETAILED DESCRIPTION OF THE INVENTION A first embodiment of the present invention will be described below with reference to FIGS. Here, as the bone filling material 11 serving as the base of the bone filling body as the living tissue filling material manufactured in the first embodiment, for example, Japanese Patent Application Laid-Open No. 5-2
A rectangular parallelepiped porous body made of β-TCP (β-tricalcium phosphate) produced by the method disclosed in 37178 is used. By applying a voltage to the surface of a ceramic porous body such as β-TCP during sintering production, the entire surface is always negatively charged even if no voltage is applied thereafter (see FIG. 1). It shows an image of being negatively charged as a whole).
【００２８】なお、このように常に負に帯電させること
ができる多孔性の生体親和材料としては、β−ＴＣＰの
他に、ＨＡＰ（ハイドロキシアパタイト）のセラミック
ス等の多孔体セラミックスがある。なお、ＰＬＡ（ポリ
乳酸）、さらには、β−ＴＣＰとＨＡＰとポリカプロラ
クトン（ＰＣＬ）の複合体等でも良い。これらのうち、
ＨＡＰは、β−ＴＣＰと同様、常に全面的に負に帯電す
るものとなり、また、β−ＴＣＰとＨＡＰとポリカプロ
ラクトン（ＰＣＬ）の複合体は、静電的な作用によって
特にポリカプロラクトンの部分が局部的に常に負に帯電
するものとなる（図２はこのように局部的に負に帯電し
ているイメージを示している）。さらに、ＨＡＰは低温
で焼結すると吸収性を高めることができるため、このよ
うなものを負に帯電させるようにしても良い。In addition to β-TCP, porous ceramics such as HAP (hydroxyapatite) ceramics are examples of porous biocompatible materials that can always be negatively charged. Note that PLA (polylactic acid), a complex of β-TCP, HAP, and polycaprolactone (PCL), or the like may be used. Of these,
Similar to β-TCP, HAP is always negatively charged on the whole surface, and the complex of β-TCP, HAP and polycaprolactone (PCL) has a polycaprolactone part particularly due to an electrostatic action. It will always be locally negatively charged (FIG. 2 shows such an image of locally negatively charged). Further, since HAP can enhance its absorbability when sintered at a low temperature, such a material may be negatively charged.
【００２９】ここで、骨補填材１１は、その気孔の大き
さおよび密度等が、骨髄液、末梢血や臍帯血等の体液の
中から所望の不要成分を通過させる一方、主に間葉系幹
細胞からなる濃縮体を捕捉可能となるように製造されて
いる。Here, the bone filling material 11 has a pore size and a density, etc., which allow desired unwanted components to pass through from body fluids such as bone marrow fluid, peripheral blood and umbilical cord blood. It is manufactured so as to be able to capture the concentrate of stem cells.
【００３０】そして、手術中に、患者の例えば腸骨から
抽出した体液である骨髄液１３を、図３に示すように上
部開口型の容器１４に入れる。次に、骨補填材１１を容
器１４内で骨髄液１３に浸して骨補填体１５とする。そ
の後、この骨補填体１５が図示略の骨欠損部に補填され
ることになる。このとき、第１実施形態の骨補填材１１
を骨髄液１３に浸すと、骨補填材１１は、自ら負に帯電
しているため、負に帯電した部分に結合しやすい細胞、
例えば間葉系幹細胞が、この骨補填材１１に容易に付着
する。したがって、手術中という限られた時間の中でも
骨補填材１１に十分に細胞が付与されることになり、そ
の結果、術後の骨欠損部の修復速度を高めることができ
る。Then, during the operation, the bone marrow fluid 13, which is a body fluid extracted from, for example, the iliac bone of a patient, is put into an upper opening type container 14 as shown in FIG. Next, the bone filling material 11 is immersed in the bone marrow fluid 13 in the container 14 to form the bone filling body 15. After that, the bone filling body 15 is filled in a bone defect portion (not shown). At this time, the bone filling material 11 of the first embodiment
When the bone filling material 11 is immersed in the bone marrow fluid 13, since the bone prosthetic material 11 is negatively charged by itself, cells that easily bond to the negatively charged portion,
For example, mesenchymal stem cells easily attach to the bone prosthetic material 11. Therefore, the cells can be sufficiently added to the bone prosthetic material 11 even during the limited time during the operation, and as a result, the speed of repair of the bone defect portion after the operation can be increased.
【００３１】なお、第１実施形態では、手術中に患者か
ら抽出した体液である骨髄液１３に骨補填材１１を浸す
場合を例にとり説明したが、骨髄液１３にかえて、患者
から抽出した体液１３を、遠心分離器や免疫磁気細胞分
離装置やフローサイトメトリー等の細胞分離装置で適宜
濃縮して所望の不要な成分を除去した濃縮体を用いた
り、この濃縮体にサイトカインや濃縮血小板等の成長因
子を適宜付与したものを用いたりすることができる。濃
縮の際には、血球成分を除去したり、遠心分離による上
澄み液を除去する。ここで、細胞分離装置としては、フ
ロー・サイトメトリーを利用したもの、あるいは磁気ビ
ーズを利用したものを用いても良い。また、骨補填材１
１を浸す細胞は、体液に含まれる細胞や、骨髄細胞の
他、間葉系幹細胞や、分化した骨細胞、骨芽細胞、破骨
細胞でも良い。加えて、これら細胞にＦＧＦやＢＭＰ、
ＴＧＦ−β、ＶＥＧＦ、ＰＤＧＦ、ＩＧＦ、ＨＧＦ等の
成長因子を加えたものでも良い。また、骨以外の軟骨細
胞や表皮細胞、真皮細胞、角膜細胞、消化管上皮や内皮
細胞、神経細胞等でも良い。In the first embodiment, the case where the bone prosthetic material 11 is soaked in the bone marrow fluid 13 which is the body fluid extracted from the patient during the operation has been described as an example, but instead of the bone marrow fluid 13, it is extracted from the patient. The body fluid 13 may be concentrated using a centrifuge, an immunomagnetic cell separation device, a cell separation device such as flow cytometry, to remove a desired unnecessary component, or a concentrated substance such as a cytokine or concentrated platelets may be used. It is also possible to use the one to which the growth factor described above is appropriately added. At the time of concentration, the blood cell components are removed and the supernatant liquid by centrifugation is removed. Here, as the cell separation device, a device using flow cytometry or a device using magnetic beads may be used. Also, bone replacement material 1
The cells soaking 1 may be cells contained in body fluid, bone marrow cells, mesenchymal stem cells, differentiated osteocytes, osteoblasts, or osteoclasts. In addition, FGF, BMP,
It may be one to which a growth factor such as TGF-β, VEGF, PDGF, IGF or HGF is added. Further, chondrocytes other than bone, epidermal cells, dermal cells, corneal cells, gastrointestinal epithelium, endothelial cells, nerve cells and the like may be used.
【００３２】次に、本発明の第２実施形態を主に図４を
参照して第１実施形態との相違部分を中心に以下に説明
する。なお、第１実施形態と同様の部分には同一の符号
を付しその説明は略す。Next, a second embodiment of the present invention will be described below mainly with reference to FIG. 4, focusing on the differences from the first embodiment. The same parts as those in the first embodiment are designated by the same reference numerals, and the description thereof will be omitted.
【００３３】第２実施形態の骨補填体の製造装置１７
は、骨髄液や末梢血や臍帯血等の体液１３が注入される
とともに骨補填材１１の断面積と実質的に同じ流路断面
積を有する通路１８が中央に形成された容器１９と、こ
の容器１９の通路１８を挟んで両側に配置された正極２
０および負極２１とこれらに電圧を印加する電源２２と
を有し通路１８内で電気泳動により体液１３を移動させ
る電気泳動部２３と、骨補填材１１が装填されるととも
に装填された骨補填材１１を通路１８内にこれを遮るよ
うに、しかもその移動を規制する状態に配置する装填部
２４とを有している。Bone prosthesis manufacturing apparatus 17 of the second embodiment
Is a container 19 in which a body fluid 13 such as bone marrow fluid, peripheral blood, or umbilical cord blood is injected, and a passage 18 having a flow passage cross-sectional area substantially the same as the cross-sectional area of the bone filling material 11 is formed in the center, Positive electrodes 2 arranged on both sides of the passage 18 of the container 19
0 and the negative electrode 21, and a power source 22 for applying a voltage to them, and an electrophoretic section 23 that moves the body fluid 13 by electrophoresis in the passage 18, and a bone filling material 11 and the loaded bone filling material. 11 has a loading section 24 arranged so as to block the passage 11 in the passage 18 and regulate the movement thereof.
【００３４】そして、手術中に、患者の例えば腸骨から
体液である骨髄液１３を抽出し、この骨髄液１３を、容
器１９に入れるとともに、装填部２４に骨補填材１１を
装填して、電気泳動部２３により骨髄液１３に電圧を印
加する。すると、骨髄液１３の間葉系幹細胞等が電気泳
動で通路１８内を正極２０から負極２１側に移動する。
その結果、間葉系幹細胞等の細胞が、骨補填材１１で捕
捉されて骨補填材１１内に留まる。このようにして、電
気泳動による移動によって間葉系幹細胞等の細胞が骨補
填材１１に送り込まれ、骨補填材１１の内部に強制的に
浸透させられて捕捉される。その結果、第１実施形態と
同様、術後の骨欠損部の修復速度を高めることができる
効果が得られる。During operation, bone marrow fluid 13, which is a body fluid, is extracted from, for example, the iliac bone of a patient, the bone marrow fluid 13 is put into a container 19, and the bone filling material 11 is loaded into the loading section 24. A voltage is applied to the bone marrow fluid 13 by the electrophoresis unit 23. Then, the mesenchymal stem cells and the like of the bone marrow fluid 13 migrate in the passage 18 from the positive electrode 20 to the negative electrode 21 side by electrophoresis.
As a result, cells such as mesenchymal stem cells are captured by the bone filling material 11 and remain in the bone filling material 11. In this way, cells such as mesenchymal stem cells are sent to the bone prosthetic material 11 by migration by electrophoresis, and are forcibly permeated into the bone prosthetic material 11 and captured. As a result, similar to the first embodiment, the effect of increasing the repair speed of the bone defect portion after surgery can be obtained.
【００３５】次に、本発明の第３実施形態を主に図５〜
図１０を参照して第１実施形態との相違部分を中心に以
下に説明する。なお、第１実施形態と同様の部分には同
一の符号を付しその説明は略す。Next, the third embodiment of the present invention will be mainly described with reference to FIGS.
The difference from the first embodiment will be mainly described below with reference to FIG. 10. The same parts as those in the first embodiment are designated by the same reference numerals, and the description thereof will be omitted.
【００３６】第３実施形態の骨補填体の製造装置２７
は、体液１３が注入される容器２８と、この容器２８に
注入された体液１３を電気泳動させる正極２９、負極３
０および電源３１を有する電気泳動部３２と、骨補填材
１１が装填されるとともに装填された骨補填材１１をそ
の移動を規制しかつ自動的に負極３０に接触させること
で骨補填材１１自体を負極とする（言い換えれば負に帯
電させる）装填部３３とを有している。Bone prosthesis manufacturing apparatus 27 of the third embodiment
Is a container 28 into which the body fluid 13 is injected, and a positive electrode 29 and a negative electrode 3 for electrophoresing the body fluid 13 injected into the container 28.
0 and the electrophoretic part 32 having the power source 31 and the bone filling material 11 are loaded, and the loaded bone filling material 11 is regulated in its movement and is automatically brought into contact with the negative electrode 30, thereby the bone filling material 11 itself. Is a negative electrode (in other words, it is negatively charged).
【００３７】そして、手術中に、患者の例えば腸骨から
体液である骨髄液１３を抽出し、この骨髄液１３を、容
器２８に入れるとともに、装填部３３に骨補填材１１を
装填して、電気泳動部３２により骨髄液１３に電圧を印
加する。すると、骨髄液１３のうち負に帯電した部分に
吸引される間葉系幹細胞等の細胞が、負極３０に接触す
ることで自ら負に帯電する骨補填材１１に容易に付着す
る。その結果、第１実施形態と同様、術後の骨欠損部の
修復速度を高めることができる効果が得られる。During operation, bone marrow fluid 13, which is a body fluid, is extracted from the patient's iliac bone, for example, and the bone marrow fluid 13 is placed in a container 28 and the bone filling material 11 is loaded in the loading portion 33. A voltage is applied to the bone marrow fluid 13 by the electrophoresis unit 32. Then, cells such as mesenchymal stem cells that are attracted to the negatively charged portion of the bone marrow fluid 13 are easily attached to the bone prosthesis material 11 that is negatively charged by contacting the negative electrode 30. As a result, similar to the first embodiment, the effect of increasing the repair speed of the bone defect portion after surgery can be obtained.
【００３８】なお、装填部３３にセットし負極３０に接
触させることで間接的に骨補填材１１を負極にするので
はなく、例えば、図７に示すように、骨補填材１１を負
極そのものとしてもよい。また、図８に示すように、負
極を構成する骨補填材１１を複数設けても良い。さら
に、図９に示すように関節部材３５が連結されることで
人工関節を構成する凸字形状の骨補填材１１を上記のよ
うに負極にすることでこの骨補填材１１に主に間葉系幹
細胞を付着させ、その後、この骨補填材１１にさらに細
胞を塗布あるいは散布等で付与しても良い。この場合、
図１０に示すように間葉系幹細胞等の細胞を付着させる
べき骨補填材１１の凸側の段状面部３６（図９および図
１０で太線で示す）を体液１３に浸けることになる。It should be noted that the bone prosthesis material 11 is not used as the negative electrode indirectly by setting it in the loading section 33 and contacting it with the negative electrode 30, but, for example, as shown in FIG. 7, the bone prosthesis material 11 is used as the negative electrode itself. Good. Further, as shown in FIG. 8, a plurality of bone prosthesis materials 11 forming the negative electrode may be provided. Further, by connecting the joint member 35 as shown in FIG. 9 to the negative electrode of the convex-shaped bone filling material 11 that constitutes the artificial joint as described above, the bone filling material 11 is mainly formed by the mesenchyme. The stem cells may be adhered, and then the cells may be applied to the bone prosthetic material 11 by coating or spraying. in this case,
As shown in FIG. 10, the convex stepped surface portion 36 (shown by the thick line in FIGS. 9 and 10) of the bone prosthetic material 11 to which cells such as mesenchymal stem cells are to be attached is immersed in the body fluid 13.
【００３９】次に、本発明の第４実施形態を主に図１１
を参照して第１実施形態との相違部分を中心に以下に説
明する。なお、第１実施形態と同様の部分には同一の符
号を付しその説明は略す。Next, the fourth embodiment of the present invention will be mainly described with reference to FIG.
The following description will be centered on the difference from the first embodiment with reference to FIG. The same parts as those in the first embodiment are designated by the same reference numerals, and the description thereof will be omitted.
【００４０】第４実施形態においては、骨補填材１１が
中央に凹部３８を有している。そして、体液１３が注入
される容器３９と、この容器３９に注入された体液１３
を電気泳動させる正極４０、負極４１および電源４２を
有する電気泳動部４３とを有する製造装置４４に、その
負極４１を凹部３８に挿入させるようにして骨補填材１
１を配置する。すなわち、電気泳動部４３の負極４１の
近傍にこの負極４１と隙間をあけて骨補填材１１を配置
する。In the fourth embodiment, the bone prosthesis material 11 has a recess 38 in the center. The container 39 into which the body fluid 13 is injected, and the body fluid 13 injected into the container 39
The negative electrode 41 is inserted into the concave portion 38 of the manufacturing apparatus 44 having the positive electrode 40 for electrophoresing the negative electrode, the negative electrode 41, and the electrophoretic portion 43 having the power source 42, and the bone filling material 1
Place 1 That is, the bone prosthesis material 11 is arranged in the vicinity of the negative electrode 41 of the electrophoretic section 43 with a gap from the negative electrode 41.
【００４１】そして、手術中に、患者の例えば腸骨から
骨髄液１３を抽出し、この骨髄液１３を、容器３９に入
れるとともに、骨補填材１１を容器３９に配置して、電
気泳動部４３により骨髄液１３に電圧を印加する。する
と、骨髄液１３は電気泳動で負に帯電した部分に吸引さ
れる間葉系幹細胞等の細胞が、負極４１に集まることで
負極４１の周囲に配置された骨補填材１１の内部に浸透
させられ骨補填材１１で捕捉される。その結果、第１実
施形態と同様、術後の骨欠損部の修復速度を高めること
ができる効果が得られる。Then, during the operation, the bone marrow fluid 13 is extracted from, for example, the iliac bone of the patient, the bone marrow fluid 13 is put into the container 39, and the bone filling material 11 is placed in the container 39. Applies a voltage to the bone marrow fluid 13. Then, the bone marrow fluid 13 causes cells such as mesenchymal stem cells, which are attracted to the negatively charged portion by electrophoresis, to collect in the negative electrode 41 and permeate into the bone prosthesis material 11 arranged around the negative electrode 41. It is captured by the bone filling material 11. As a result, similar to the first embodiment, the effect of increasing the repair speed of the bone defect portion after surgery can be obtained.
【００４２】次に、本発明の第５実施形態を主に図１２
を参照して第１実施形態との相違部分を中心に以下に説
明する。なお、第１実施形態と同様の部分には同一の符
号を付しその説明は略す。Next, the fifth embodiment of the present invention will be mainly described with reference to FIG.
The following description will be centered on the difference from the first embodiment with reference to FIG. The same parts as those in the first embodiment are designated by the same reference numerals, and the description thereof will be omitted.
【００４３】第５実施形態においては、骨補填材１１が
その中央に凹形状の注入空間４７を有している。そし
て、骨補填材１１が挿入されることによりその外面に接
触する容器状の負極４８と、この負極４８に接続される
電源４９と、この電源４９に接続される棒状の正極５０
とを有する製造装置５１の負極４８に骨補填材１１を挿
入しておく。In the fifth embodiment, the bone prosthetic material 11 has a concave injection space 47 in the center thereof. The container-shaped negative electrode 48 that comes into contact with the outer surface of the bone filling material 11 when it is inserted therein, the power source 49 connected to the negative electrode 48, and the rod-shaped positive electrode 50 connected to the power source 49.
The bone filling material 11 is inserted into the negative electrode 48 of the manufacturing apparatus 51 having the above.
【００４４】そして、手術中に、患者の例えば腸骨から
体液である骨髄液１３を抽出し、この骨髄液１３を、骨
補填材１１の注入空間４７に注入するとともに、正極５
０をこの注入空間４７の骨髄液１３に挿入する。する
と、骨髄液１３のうち負に帯電した部分に吸引される間
葉系幹細胞等の細胞が、負極４８に向かって移動し、骨
補填材１１の内部に浸透させられる。その結果、第１実
施形態と同様、術後の骨欠損部の修復速度を高めること
ができる効果が得られる。During operation, bone marrow fluid 13, which is a body fluid, is extracted from the patient's iliac bone, for example, and the bone marrow fluid 13 is injected into the injection space 47 of the bone filling material 11 and the positive electrode 5
0 is inserted into the bone marrow fluid 13 in the injection space 47. Then, cells such as mesenchymal stem cells attracted to the negatively charged portion of the bone marrow fluid 13 move toward the negative electrode 48 and permeate into the bone prosthesis material 11. As a result, similar to the first embodiment, the effect of increasing the repair speed of the bone defect portion after surgery can be obtained.
【００４５】[0045]
【発明の効果】以上詳述したように、本発明の生体組織
補填材によれば、負に帯電しているため、負に帯電した
部分に結合しやすい細胞が容易に付着する。したがっ
て、限られた時間の中でも十分に細胞が付与されること
になり、その結果、術後の生体組織欠損部の修復速度を
高めることができる。As described in detail above, according to the biological tissue filling material of the present invention, since it is negatively charged, cells that easily bond to the negatively charged portion easily attach. Therefore, the cells are sufficiently added even in a limited time, and as a result, the rate of repair of the biological tissue defect portion after surgery can be increased.
【００４６】本発明の生体組織補填体によれば、生体組
織補填材が負に帯電しているため、負に帯電した部分に
結合しやすい細胞が容易に付着する。したがって、限ら
れた時間の中でも十分に生体組織補填材に細胞が付与さ
れることになり、その結果、術後の生体組織欠損部の修
復速度を高めることができる。According to the biological tissue filling material of the present invention, since the living tissue filling material is negatively charged, cells that easily bond to the negatively charged portion easily attach. Therefore, the cells can be sufficiently added to the biological tissue filling material even within a limited time, and as a result, the rate of repair of the biological tissue defective portion after surgery can be increased.
【００４７】本発明の生体組織補填体の製造方法によれ
ば、細胞を容器内で電気泳動により移動させ、該移動に
より生体組織補填材に細胞を浸透させるため、生体組織
補填材の内部に細胞が強制的に浸透させられる。したが
って、限られた時間の中でも、生体組織補填材に十分に
細胞が付与された生体組織補填体を得ることができるこ
とになり、その結果、術後の生体組織欠損部の修復速度
を高めることができる。According to the method for producing a biological tissue filling material of the present invention, the cells are moved by electrophoresis in the container and the migration causes the cells to permeate into the biological tissue filling material. Is forced to penetrate. Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００４８】本発明の生体組織補填体の製造方法によれ
ば、電気泳動用の負極の近傍に生体組織補填材を配置す
るため、電気泳動で負に帯電した部分に結合しやすい細
胞が生体組織補填材に集まって浸透させられる。したが
って、限られた時間の中でも、生体組織補填材に一層多
くの細胞が付与された生体組織補填体を得ることができ
ることになり、その結果、術後の生体組織欠損部の修復
速度をさらに高めることができる。According to the method for producing a living tissue filling of the present invention, the living tissue filling material is arranged in the vicinity of the negative electrode for electrophoresis, so that cells that easily bind to the negatively charged portion by electrophoresis are living tissue. It gathers in the filling material and is permeated. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to.
【００４９】本発明の生体組織補填体の製造方法によれ
ば、電気泳動用の負極として生体組織補填材を配置する
ため、電気泳動で負に帯電した部分に結合しやすい細胞
が生体組織補填材に集まって浸透させられる。したがっ
て、限られた時間の中でも、生体組織補填材に一層多く
の細胞が付与された生体組織補填体を得ることができる
ことになり、その結果、術後の生体組織欠損部の修復速
度をさらに高めることができる。According to the method for producing a biological tissue filling material of the present invention, since the biological tissue filling material is arranged as the negative electrode for electrophoresis, the cells which are easily bonded to the negatively charged portion by the electrophoresis are the biological tissue filling material. Gathered in and penetrated. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to.
【００５０】本発明の生体組織補填体の製造方法によれ
ば、生体組織補填材の外面に配置された負極と生体組織
補填材の注入空間に注入された細胞に配置された正極と
に電圧を印加すると、負に帯電した部分に結合しやすい
細胞が生体組織補填材を内側から外側に向けて移動させ
られて生体組織補填材に浸透させられる。したがって、
限られた時間の中でも、生体組織補填材に十分に細胞が
付与された生体組織補填体を得ることができることにな
り、その結果、術後の生体組織欠損部の修復速度を高め
ることができる。According to the method for producing a biological tissue filling material of the present invention, a voltage is applied to the negative electrode arranged on the outer surface of the biological tissue filling material and the positive electrode arranged in the cells injected into the injection space of the biological tissue filling material. When applied, cells that tend to bind to the negatively charged portion are moved from the inside of the biological tissue filling material toward the outside and are permeated into the biological tissue filling material. Therefore,
Even within a limited time, it is possible to obtain a living tissue filling body in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery.
【００５１】本発明の生体組織補填体の製造方法によれ
ば、生体組織補填材を負に帯電させるため、生体組織補
填材に塗布あるいは散布等された、負に帯電した部分に
結合しやすい細胞が生体組織補填材に容易に付着する。
したがって、限られた時間の中でも、生体組織補填材に
十分に細胞が付与された生体組織補填体を得ることがで
きることになり、その結果、術後の生体組織欠損部の修
復速度を高めることができる。According to the method for producing a biological tissue filling material of the present invention, since the living tissue filling material is negatively charged, cells that are easily applied to the negatively charged portion that is applied or sprayed on the biological tissue filling material. Easily adheres to the biological tissue filling material.
Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００５２】本発明の生体組織補填体の製造装置によれ
ば、装填部に生体組織補填材を装填すると、生体組織補
填材が自動的に電気泳動で細胞を移動させる通路に配置
される。よって、通路で細胞を電気泳動により移動させ
ると生体組織補填材に細胞が強制的に浸透させられる。
したがって、限られた時間の中でも、生体組織補填材に
十分に細胞が付与された生体組織補填体を得ることがで
きることになり、その結果、術後の生体組織欠損部の修
復速度を高めることができる。According to the living tissue filling production apparatus of the present invention, when the loading portion is loaded with the living tissue filling material, the living tissue filling material is automatically arranged in the passage for moving the cells by electrophoresis. Therefore, when the cells are electrophoretically moved in the passage, the cells are forcibly permeated into the biological tissue filling material.
Therefore, even within a limited time, it is possible to obtain a living tissue filling material in which cells are sufficiently added to the living tissue filling material, and as a result, it is possible to increase the repair speed of the living tissue defective portion after surgery. it can.
【００５３】本発明の生体組織補填体の製造装置によれ
ば、装填部に生体組織補填材を装填すると、生体組織補
填材が自動的に電気泳動用の負極に接触させられ、電気
泳動で負に帯電した部分に結合しやすい細胞が生体組織
補填材に集まって浸透させられる。したがって、限られ
た時間の中でも、生体組織補填材に一層多くの細胞が付
与された生体組織補填体を得ることができることにな
り、その結果、術後の生体組織欠損部の修復速度をさら
に高めることができる。しかも、生体組織補填材が装填
部への装填で自動的に電気泳動用の負極に接触させられ
るため、生体組織補填材をセットする作業が容易とな
る。According to the apparatus for producing a living tissue filling of the present invention, when the loading portion is loaded with the living tissue filling material, the living tissue filling material is automatically brought into contact with the negative electrode for electrophoresis, and the negative electrode is electrophoresed. Cells that tend to bind to the electrically charged parts gather in the tissue filling material and are permeated. Therefore, it is possible to obtain a living tissue filling material in which more cells are added to the living tissue filling material even within a limited time, and as a result, the repair speed of the living tissue defective portion after operation is further increased. be able to. Moreover, since the biological tissue filling material is automatically brought into contact with the negative electrode for electrophoresis when loaded in the loading portion, the work of setting the biological tissue filling material becomes easy.
【図１】 本発明の第１実施形態の生体組織補填材につ
いてその一例の帯電状態をイメージ的に示す斜視図であ
る。FIG. 1 is a perspective view conceptually showing a charged state of an example of a biological tissue filling material according to a first embodiment of the present invention.
【図２】 本発明の第１実施形態の生体組織補填材につ
いてその別の例の帯電状態をイメージ的に示す斜視図で
ある。FIG. 2 is a perspective view conceptually showing a charged state of another example of the biological tissue filling material according to the first embodiment of the present invention.
【図３】 本発明の第１実施形態の生体組織補填体の製
造過程を示す側断面図である。FIG. 3 is a side sectional view showing a manufacturing process of the biological tissue filling body according to the first embodiment of the present invention.
【図４】 本発明の第２実施形態の生体組織補填体の製
造装置を示す正断面図である。FIG. 4 is a front cross-sectional view showing a biological tissue filling device manufacturing apparatus according to a second embodiment of the present invention.
【図５】 本発明の第３実施形態の生体組織補填体の製
造装置を示す正断面図である。FIG. 5 is a front sectional view showing a device for manufacturing a biological tissue filling body according to a third embodiment of the present invention.
【図６】 本発明の第３実施形態の生体組織補填体の製
造装置を示す平面図である。FIG. 6 is a plan view showing an apparatus for manufacturing a biological tissue filling body according to a third embodiment of the present invention.
【図７】 本発明の第３実施形態の生体組織補填体の製
造装置についての変形例１を示す正断面図である。FIG. 7 is a front cross-sectional view showing a modified example 1 of the device for manufacturing a biological tissue filler according to the third embodiment of the present invention.
【図８】 本発明の第３実施形態の生体組織補填体の製
造装置についての変形例２を示す正断面図である。FIG. 8 is a front cross-sectional view showing a modified example 2 of the apparatus for manufacturing a biological tissue filler according to the third embodiment of the present invention.
【図９】 本発明の第３実施形態の生体組織補填体の製
造装置で製造可能な生体組織補填体を含む人工関節を示
す平面図である。FIG. 9 is a plan view showing an artificial joint including a biological tissue filling body that can be manufactured by the biological tissue filling body manufacturing apparatus according to the third embodiment of the present invention.
【図１０】 図９に示す生体組織補填体用の製造装置を
示す正断面図である。10 is a front cross-sectional view showing a manufacturing apparatus for a biological tissue filling body shown in FIG.
【図１１】 本発明の第４実施形態の生体組織補填体の
製造装置を示す平面図である。FIG. 11 is a plan view showing an apparatus for manufacturing a biological tissue filler according to a fourth embodiment of the present invention.
【図１２】 本発明の第５実施形態の生体組織補填体の
製造装置を示す平面図である。FIG. 12 is a plan view showing an apparatus for manufacturing a biological tissue filling body according to a fifth embodiment of the present invention.
１１ 骨補填材（生体組織補填材） １３ 体液（細胞） １５ 骨補填体（生体組織補填体） １７，２７ 製造装置 １８ 通路 １９，２８ 容器 ２３ 電気泳動部 ２４，３３ 装填部 ２９ 正極 ３０，４１，４８ 負極 ４７ 注入空間 ５０ 正極 11 Bone filling material (living tissue filling material) 13 Body fluid (cell) 15 Bone filling material (living tissue filling material) 17,27 Manufacturing equipment 18 passages 19,28 containers 23 Electrophoresis unit 24, 33 loading section 29 Positive electrode 30, 41, 48 Negative electrode 47 injection space 50 positive electrode
フロントページの続き (72)発明者 日比野 浩樹 東京都渋谷区幡ヶ谷２丁目43番２号 オリ ンパス光学工業株式会社内 Ｆターム(参考） 4C081 AB04 BA02 BA12 BA13 BA17 CF021 CF031 DA01 EA06Continued front page (72) Inventor Hiroki Hibino 2-43 Hatagaya, Shibuya-ku, Tokyo Ori Inside Npus Optical Industry Co., Ltd. F-term (reference) 4C081 AB04 BA02 BA12 BA13 BA17 CF021 CF031 DA01 EA06
Claims (12)
補填される生体組織補填材において、少なくともその一
部が負に帯電していることを特徴とする生体組織補填
材。1. A living tissue filling material to be filled in a living tissue defective portion after cells are added, wherein at least a part of the living tissue filling material is negatively charged.
とを特徴とする請求項１記載の生体組織補填材。2. The living tissue filling material according to claim 1, wherein the living tissue defective portion is a bone defective portion.
され、生体組織欠損部に補填される生体組織補填体にお
いて、前記生体組織補填材の少なくとも一部が負に帯電
していることを特徴とする生体組織補填体。3. A living tissue filling body configured by adding cells to a living tissue filling material and filling a living tissue defective portion, wherein at least a part of the living tissue filling material is negatively charged. A characteristic biological tissue supplement.
とを特徴とする請求項３記載の生体組織補填体。4. The body tissue filling member according to claim 3, wherein the body tissue defect portion is a bone defect portion.
せることにより、生体組織欠損部に補填される生体組織
補填体を形成する生体組織補填体の製造方法であって、 前記細胞を容器内で電気泳動により移動させ、該移動に
より前記生体組織補填材に前記細胞を浸透させることを
特徴とする生体組織補填体の製造方法。5. A method for producing a biological tissue filling body which forms a living tissue filling body to be filled in a living tissue defective portion by infiltrating cells into a porous living tissue filling material, wherein the cells are contained in a container. A method for producing a biological tissue filling body, which comprises migrating the inside of the body tissue by electrophoresis and causing the cells to permeate the living tissue filling material by the movement.
組織補填材を配置することを特徴とする請求項５記載の
生体組織補填体の製造方法。6. The method for producing a biological tissue filling body according to claim 5, wherein the biological tissue filling material is arranged in the vicinity of the negative electrode for electrophoresis.
負極とすることを特徴とする請求項５記載の生体組織補
填体の製造方法。7. The method for producing a biological tissue filling body according to claim 5, wherein the living tissue filling material is used as the negative electrode for electrophoresis.
せることにより、生体組織欠損部に補填される生体組織
補填体を形成する生体組織補填体の製造方法であって、 前記生体組織補填材の内側に前記細胞が注入される注入
空間を形成し、 前記生体組織補填材の外面に負極を配置するとともに前
記注入空間に注入された前記細胞に正極を配置して電圧
を印加することを特徴とする生体組織補填体の製造方
法。8. A method for producing a living tissue filling body, which comprises forming a living tissue filling body to be filled in a living tissue defective portion by infiltrating cells into a porous living tissue filling material, the living tissue filling body comprising: Forming an injection space into which the cells are injected inside the material, arranging a negative electrode on the outer surface of the biological tissue filling material, and arranging a positive electrode to the cells injected into the injection space to apply a voltage. A method for producing a characteristic biological tissue filler.
より、生体組織欠損部に補填される生体組織補填体を形
成する生体組織補填体の製造方法であって、 前記生体組織補填材を負に帯電させることを特徴とする
生体組織補填体の製造方法。9. A method for producing a biological tissue filling body, which comprises forming a biological tissue filling body to be filled in a living tissue defective portion by applying cells to the living tissue filling material, wherein the living tissue filling material is negative. A method for producing a body tissue filling body, comprising:
により、生体組織欠損部に補填される生体組織補填体を
形成する生体組織補填体の製造装置であって、 前記生体組織補填材を負に帯電させることを特徴とする
生体組織補填体の製造装置。10. An apparatus for producing a living tissue filling body, which forms a living tissue filling body to be filled in a living tissue defective portion by applying cells to the living tissue filling material, wherein the living tissue filling material is negative. An apparatus for producing a body tissue filling body, which is characterized in that the body tissue is charged.
させることにより、生体組織欠損部に補填される生体組
織補填体を形成する生体組織補填体の製造装置であっ
て、 前記細胞が注入されるとともに通路を有する容器と、該
容器の前記通路内で電気泳動により前記細胞を移動させ
る電気泳動部と、装填された前記生体組織補填材を前記
通路に配置する装填部とを有することを特徴とする生体
組織補填体の製造装置。11. An apparatus for producing a biological tissue filling body, which forms a biological tissue filling body to be filled in a living tissue defective portion by infiltrating cells into a porous living tissue filling material, wherein the cells are injected. A container having a passage and a passage, an electrophoretic portion for moving the cells by electrophoresis in the passage of the container, and a loading portion for arranging the loaded biological tissue filling material in the passage. A device for producing a characteristic biological tissue filling body.
させることにより、生体組織欠損部に補填される生体組
織補填体を形成する生体組織補填体の製造装置であっ
て、 前記細胞が注入される容器と、該容器に注入された前記
細胞を電気泳動させる正極および負極と、装填された前
記生体組織補填材を前記負極に接触させる装填部とを有
することを特徴とする生体組織補填体の製造装置。12. A device for producing a living tissue filling body, which forms a living tissue filling body to be filled in a living tissue defective portion by infiltrating cells into a porous living tissue filling material, wherein the cells are injected. And a negative electrode for electrophoresing the cells injected into the container, and a loading part for bringing the loaded biological tissue filling material into contact with the negative electrode. Manufacturing equipment.
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|---|---|---|---|
| JP2002129263A JP2003320016A (en) | 2002-04-30 | 2002-04-30 | Living tissue filling material, living tissue filling body, and method and device of manufacturing living tissue filling body |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002129263A JP2003320016A (en) | 2002-04-30 | 2002-04-30 | Living tissue filling material, living tissue filling body, and method and device of manufacturing living tissue filling body |
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|---|---|
| JP2003320016A true JP2003320016A (en) | 2003-11-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005168360A (en) * | 2003-12-09 | 2005-06-30 | Olympus Corp | Method for examining biological tissue-supplying material, device, cell culture container and method for examining culturing state |
| JP2014103460A (en) * | 2012-11-16 | 2014-06-05 | Jai Corporation | Image reading apparatus with aberration correction function |
-
2002
- 2002-04-30 JP JP2002129263A patent/JP2003320016A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005168360A (en) * | 2003-12-09 | 2005-06-30 | Olympus Corp | Method for examining biological tissue-supplying material, device, cell culture container and method for examining culturing state |
| JP2014103460A (en) * | 2012-11-16 | 2014-06-05 | Jai Corporation | Image reading apparatus with aberration correction function |
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