JP2003313130A - Inhibitor against oxidation damage to gene - Google Patents
Inhibitor against oxidation damage to geneInfo
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- JP2003313130A JP2003313130A JP2002116726A JP2002116726A JP2003313130A JP 2003313130 A JP2003313130 A JP 2003313130A JP 2002116726 A JP2002116726 A JP 2002116726A JP 2002116726 A JP2002116726 A JP 2002116726A JP 2003313130 A JP2003313130 A JP 2003313130A
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- gene
- oxidative damage
- molecular weight
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【発明が属する技術分野】本発明は、様々な因子により
引き起こされる遺伝子の酸化損傷を有効に抑制する物
質、特に核中に含まれる核タンパク質を分解して得られ
る水溶性の低分子化核タンパク質に関する。TECHNICAL FIELD The present invention relates to a substance which effectively suppresses oxidative damage of genes caused by various factors, particularly a water-soluble low molecular weight nuclear protein obtained by degrading nuclear protein contained in the nucleus. Regarding
【０００２】[0002]
【従来の技術】生物の遺伝情報がアデニン、チミン、グ
アニンおよびシトシンのヌクレオチドを構成要素とする
遺伝子により暗号化されていることは、もはや周知の事
実である。ところが、遺伝子は紫外線照射、各種の化学
物質、特に発癌物質および活性酸素種への暴露等により
一定頻度で酸化損傷を受けて、複製エラーが生じること
が近年の研究により明らかになった。該複製エラーは遺
伝情報の劣化を生じ、そして細胞や個体の各種疾患や死
滅の原因となる。従って、ある物質が遺伝子の酸化損傷
を促進するかそれとも抑制するかを調査することによ
り、その物質が生物に対して有害性または有効性のいず
れを有するかを判断することができると考えられる。2. Description of the Related Art It is a well-known fact that the genetic information of an organism is encoded by a gene whose nucleotides are adenine, thymine, guanine and cytosine. However, recent studies have revealed that genes undergo oxidative damage at a constant frequency due to ultraviolet irradiation, exposure to various chemical substances, particularly carcinogens and reactive oxygen species, and replication errors occur. The replication error causes deterioration of genetic information and causes various diseases and death of cells and individuals. Therefore, by investigating whether a substance promotes or suppresses the oxidative damage of genes, it is possible to judge whether the substance is harmful to the organism or effective.
【０００３】遺伝子損傷を引き起こす頻度を決定するこ
とによる物質の生物学的評価法としては、国際公開０１
６９２３５号公報に記載のものが挙げられる。該方法で
は、遺伝子を構成するモノヌクレオシドの酸化に着目
し、被検物質を含有する溶液に２’−デオキシグアノシ
ン（ｄＧ）を添加し、一定時間経過および／または紫外
線照射等の酸化負荷を加えた後、該溶液中に存在するｄ
Ｇの酸化体：８−ヒドロキシ−２’−デオキシグアノシ
ン（８ＯＨｄＧ）の濃度を測定する。そして、生成した
８ＯＨｄＧの量に基づいて、被検物質の有害性または有
効性の有無およびその程度を決定する。８ＯＨｄＧの生
成量が多ければ多いほど被検物質の有害性は高く、一
方、８ＯＨｄＧの生成量が少なければ少ないほど被検物
質の有効性は高いこととなる。As a biological evaluation method for a substance by determining the frequency of causing gene damage, International Publication 01
Examples thereof include those described in Japanese Patent No. 69235. In the method, attention is paid to the oxidation of mononucleosides constituting a gene, 2′-deoxyguanosine (dG) is added to a solution containing a test substance, and a certain period of time and / or an oxidative load such as ultraviolet irradiation is added. D present in the solution
The concentration of the oxidant of G: 8-hydroxy-2′-deoxyguanosine (8OHdG) is measured. Then, based on the amount of generated 8OHdG, the presence or absence and the degree of toxicity or effectiveness of the test substance are determined. The greater the amount of 8OHdG produced, the higher the toxicity of the test substance, while the smaller the amount of 8OHdG produced, the higher the effectiveness of the test substance.
【０００４】[0004]
【発明が解決しようとする結果】ところで、現在、天然
素材をエキス化したものおよび特定の物質を許容量の範
囲内で添加したものを主として、様々な種類の健康食品
が販売されている。しかしながら現在まで、健康食品の
有効性を客観的に評価し得る方法は確立されておらず、
そのため、健康食品には確かな有効性を示すものと実際
には有効性を示さないものとが混在しているのが現状で
ある。By the way, various kinds of health foods are currently on the market, mainly those obtained by extracting natural materials and those obtained by adding specific substances within an allowable range. However, to date, no method has been established that can objectively evaluate the effectiveness of health foods,
Therefore, in the present situation, healthy foods are mixed with those showing certain effectiveness and those not actually showing effectiveness.
【０００５】従って、物質の遺伝子損傷を引き起こす程
度を評価し、遺伝子損傷を抑制する物質を含み確実な有
効性を示す健康食品を提供することが望まれている。[0005] Therefore, it is desired to evaluate the extent to which a substance causes gene damage and to provide a health food containing a substance that suppresses gene damage and showing certain effectiveness.
【０００６】また、水、一般食品、化学品、医薬品、食
品添加物等、経口的に摂取する物質が数多くあり、該物
質が遺伝子損傷して病気・老化を引き起こすものか、ま
たは遺伝子を守るものであるかを知ることは重要であ
る。In addition, there are many substances to be taken orally, such as water, general foods, chemicals, pharmaceuticals, food additives, etc., which cause gene damage and cause illness / aging, or those which protect genes. It is important to know what is.
【０００７】[0007]
【課題を解決するための手段】上記の課題に鑑み、本発
明者等は上記のｄＧ・８ＯＨｄＧを利用する生物学的評
価法により、様々な物質の有害性／有効性について評価
を行った。その結果、生物細胞の核中に含まれる核タン
パク質を酵素により分解して得られる低分子化核タンパ
ク質が遺伝子の酸化損傷を良好に抑制することを見出し
て本発明を完成させた。In view of the above problems, the present inventors have evaluated the harmfulness / effectiveness of various substances by the biological evaluation method utilizing the above dG · 8OHdG. As a result, they have found that a low molecular weight nuclear protein obtained by decomposing a nuclear protein contained in the nucleus of a biological cell with an enzyme suppresses oxidative damage of a gene well, and completed the present invention.
【０００８】上記の課題を解決するために、本発明の請
求項１に係る遺伝子酸化損傷抑制剤は、核タンパク質を
ヌクレアーゼおよびプロテアーゼで処理して得られる低
分子化したオリゴヌクレオチド／ヌクレオシドおよびオ
リゴペプチドを３０％以上含むことを特徴とする。本発
明の遺伝子酸化損傷抑制剤は、紫外線照射、化学物質へ
の暴露等の酸化負荷が加えられたときに、遺伝子の酸化
損傷を良好に抑制することができる。In order to solve the above problems, the gene oxidative damage inhibitor according to claim 1 of the present invention is a low molecular weight oligonucleotide / nucleoside and oligopeptide obtained by treating a nuclear protein with nuclease and protease. Is included in an amount of 30% or more. The gene oxidative damage inhibitor of the present invention can satisfactorily suppress gene oxidative damage when an oxidative load such as ultraviolet irradiation or exposure to a chemical substance is applied.
【０００９】本発明の請求項２に係る遺伝子酸化損傷抑
制剤は、請求項１に記載の遺伝子酸化損傷抑制剤におい
て、魚類の白子より製造することを特徴とする。魚類の
白子は、ごく一部が食用として利用されているのみで、
大部分は廃棄処分されている未利用の資源である。しか
し白子はＤＮＡを主成分とする核酸およびプロタミンを
主成分とするタンパク質を多く含有しているので、白子
から本発明の遺伝子酸化損傷抑制剤を効率良く製造し得
るのみならず、プロタミンに由来する効果も具備した製
品とすることができる。The gene oxidative damage inhibitor according to claim 2 of the present invention is the gene oxidative damage inhibitor according to claim 1, which is characterized in that it is produced from fish milts. Only a small part of the fish Shirako is used for food,
Most are unused resources that have been disposed of. However, since algae contains a large amount of nucleic acids containing DNA as a main component and proteins containing protamine as a main component, not only can the gene oxidation inhibitor of the present invention be efficiently produced from algae, but it is also derived from protamine. It can be a product having the effect.
【００１０】本発明の請求項３に係る食品は、請求項１
に記載の遺伝子酸化損傷抑制剤を含むことを特徴とす
る。本発明の遺伝子酸化損傷抑制剤は良好な遺伝子酸化
損傷の抑制作用を有するので、食品、水、食品添加物、
化学品および医薬品に混入することにより確かな有効性
を示す製品を製造することができる。The food according to claim 3 of the present invention is the food according to claim 1.
And a gene oxidative damage inhibitor as described in 1. Since the gene oxidative damage inhibitor of the present invention has a good effect of suppressing gene oxidative damage, food, water, food additives,
By mixing with chemicals and pharmaceuticals, it is possible to manufacture products that show certain effectiveness.
【００１１】本発明の請求項４に係る方法は、食品、
水、食品添加物、化学品および医薬品が有する遺伝子酸
化損傷作用を抑制する方法であって、該食品、水、食品
添加物、化学品および医薬品に遺伝子酸化損傷抑制有効
量の請求項１に記載の遺伝子酸化損傷抑制剤を添加する
ことからなる。本発明の遺伝子酸化損傷抑制剤は良好な
遺伝子損傷の抑制作用を有するので、これを遺伝子酸化
損傷を引き起こし易い食品、水、食品添加物、化学品お
よび医薬品に混入することにより、それらが有する遺伝
子酸化損傷作用を低減することができる。The method according to claim 4 of the present invention is a food product,
A method for suppressing the gene oxidative damage effect of water, food additives, chemicals and pharmaceuticals, wherein the food, water, food additives, chemicals and pharmaceuticals are effective in suppressing gene oxidative damage. Of the gene oxidative damage. Since the gene oxidative damage inhibitor of the present invention has a good effect of suppressing gene damage, by mixing it with foods, water, food additives, chemicals and pharmaceuticals that easily cause gene oxidative damage, The oxidative damage effect can be reduced.
【００１２】[0012]
【発明の実施の形態】本発明の遺伝子酸化損傷抑制剤は
低分子化した核タンパク質からなり、該核タンパク質
は、生物細胞の核中に含まれる核タンパク質をヌクレア
ーゼおよびプロテアーゼで処理して低分子化することに
より得られる。元来、細胞核中に含まれる核酸およびタ
ンパク質は高分子のため水不溶性であるが、このように
低分子化することにより水可溶性となり、ドリンク剤等
の広い用途に用いることができ、また消化吸収力が弱い
人にも有効に使用することが可能となる。しかしなが
ら、核酸およびタンパク質を際限無しに低分子化する
と、最終的にはモノヌクレオチド／ヌクレオシドおよび
アミノ酸にまで分解されてしまい、本来の核酸およびタ
ンパク質が示す有効性が失われる。従って、本発明の低
分子化核タンパク質では、低分子化の程度を、オリゴヌ
クレオチド／ヌクレオシドおよびオリゴペプチドの分子
量が１０００〜３０００のものが含まれる程度にする。
このように核中の核酸およびタンパク質をその分子量が
１０００〜３０００となるように低分子化することによ
り、本来の有効性を保持したまま消化を容易にすること
ができる。BEST MODE FOR CARRYING OUT THE INVENTION The gene oxidative damage-suppressing agent of the present invention comprises a reduced molecular weight nuclear protein, which is obtained by treating a nuclear protein contained in the nucleus of a biological cell with a nuclease and a protease. It can be obtained by Originally, nucleic acids and proteins contained in the cell nucleus are water-insoluble due to their high molecular weight, but by reducing the molecular weight, they become water-soluble and can be used for a wide range of purposes such as drinks, digestion and absorption. It becomes possible to use it effectively even for a weak person. However, if the nucleic acids and proteins are infinitely reduced in molecular weight, they will eventually be decomposed into mononucleotides / nucleosides and amino acids, and the original effectiveness of nucleic acids and proteins will be lost. Therefore, in the miniaturized nucleoprotein of the present invention, the degree of miniaturization is set to the extent that oligonucleotides / nucleosides and oligopeptides having a molecular weight of 1000 to 3000 are included.
Thus, by lowering the molecular weight of the nucleic acid and protein in the nucleus so that the molecular weight thereof becomes 1000 to 3000, digestion can be facilitated while maintaining the original effectiveness.
【００１３】このように低分子化した核タンパク質は、
その結果、水溶性をも兼備することとなる。従来の核中
の核酸およびタンパク質を成分とした製剤は、それらが
高い分子量を有するため水に不溶であり、錠剤、顆粒、
カプセル剤等の形態で摂取する以外なかった。しかしな
がら、本発明の低分子化核タンパク質は水溶性であるた
め飲料や流動食に容易に配合することができる。ここで
いう水溶性とは、水に０．１％以上の濃度で溶解し得る
性質である。The nuclear protein having a low molecular weight is
As a result, it also has water solubility. Conventional formulations containing nucleic acids and proteins in the nucleus are insoluble in water because of their high molecular weight, tablets, granules,
There was nothing but ingestion in the form of capsules. However, since the low molecular weight nuclear protein of the present invention is water-soluble, it can be easily incorporated into beverages and liquid foods. The term "water-soluble" as used herein means a property of being soluble in water at a concentration of 0.1% or more.
【００１４】このようにして得られる本発明の遺伝子酸
化損傷抑制剤は良好な遺伝子酸化損傷抑制作用を有す
る。本発明における遺伝子酸化損傷とは核酸、ひいては
核酸を構成する最小単位であるモノヌクレオチド／ヌク
レオシド、例えばｄＧが、紫外線照射、化学物質への暴
露等の酸化負荷によりその酸化体、例えばｄＧの場合に
は８ＯＨｄＧに酸化されることを意味する。遺伝子酸化
損傷は遺伝子に暗号化された遺伝情報の劣化を生じ、そ
して各種疾患や死滅の原因となる。また、遺伝子酸化損
傷抑制作用とは、遺伝子に対して酸化負荷が加えられた
ときに、その酸化損傷を抑制する作用を意味する。The gene oxidative damage inhibitor of the present invention thus obtained has a good gene oxidative damage suppressive action. The term “genetic oxidation damage” as used in the present invention refers to a case where a nucleic acid, and thus a mononucleotide / nucleoside, which is a minimum unit constituting a nucleic acid, such as dG, is an oxidant such as dG due to an oxidative load such as ultraviolet irradiation or exposure to chemical substances Means that it is oxidized to 8OHdG. Gene oxidative damage results in the deterioration of genetic information encoded in genes and causes various diseases and death. The gene oxidative damage inhibitory action means an action of suppressing the oxidative damage when an oxidative load is applied to a gene.
【００１５】魚類の核はアルギニンを主成分とするタン
パク質であるプロタミンを多く含み、そしてプロタミン
を分解して得られるオリゴペプチドは抗アレルギー作
用、美肌作用等の優れた効果を示すことが知られてい
る。また核酸には、美肌、生活習慣病の予防と改善効果
がある。従って、魚類の白子を原料として本発明の低分
子化核タンパク質を製造することにより、遺伝子酸化損
傷抑制作用に加えて、核酸とプロタミン由来の効果をも
兼備した製品を得ることができる。該魚類とは例えば
鮭、鰊、鱒、鱈等であり、これらの白子から皮、筋、血
管等を除去した後、精製して油分を除き、ヌクレアーゼ
およびプロテアーゼでの処理を行うことにより、本発明
の低分子化核タンパク質を製造することができる。It is known that the core of fish contains a large amount of protamine, which is a protein whose main component is arginine, and the oligopeptide obtained by degrading protamine exhibits excellent effects such as antiallergic action and skin beautifying action. There is. Nucleic acid has the effect of preventing and improving beautiful skin and lifestyle-related diseases. Therefore, by producing the low molecular weight nuclear protein of the present invention using fish milt as a raw material, it is possible to obtain a product having not only an effect of suppressing gene oxidative damage but also an effect derived from nucleic acid and protamine. The fish is, for example, salmon, gills, trout, cod, etc., and after removing skin, muscle, blood vessels, etc. from these algae, it is purified to remove oil and treated with nuclease and protease The miniaturized nuclear protein of the invention can be produced.
【００１６】本発明の遺伝子酸化損傷抑制剤は水溶性の
低分子化核タンパク質からなるので、飲料、流動食等に
そのまま配合することができる。また、乾燥固化させ
て、錠剤、顆粒、カプセル剤等の剤型にすることも可能
である。本発明の低分子化核タンパク質は、上記したよ
うに低分子化されているため、たとえ固形であっても従
来品に比べて各段に消化し易い。本発明の低分子化核タ
ンパク質の摂取量は例えば、錠剤および顆粒の場合、核
酸換算で３００〜１，０００ｍｇ／日、またドリンク剤
の場合、核酸換算で５０〜１００ｍｇ／日が好ましい。Since the gene oxidative damage inhibitor of the present invention comprises a water-soluble low molecular weight nuclear protein, it can be directly incorporated into beverages, liquid foods and the like. It is also possible to dry and solidify it to obtain tablets, granules, capsules and the like. Since the low molecular weight nuclear protein of the present invention has a low molecular weight as described above, even if it is solid, it is more easily digested at each stage than conventional products. The ingestion amount of the low molecular weight nuclear protein of the present invention is preferably 300 to 1,000 mg / day in terms of nucleic acid in the case of tablets and granules, and 50 to 100 mg / day in terms of nucleic acid in the case of a drink.
【００１７】本発明の低分子化核タンパク質を含有する
食品は特に限定されず、例えば、食品、水、食品添加
物、化学品および医薬品であることができる。また配合
の方法は、従来の固形としての配合方法に加え、配合す
る対象が一定の水分を含む場合には、そのまま配合して
該水分中に溶解させることもできる。The food containing the low molecular weight nucleoprotein of the present invention is not particularly limited and may be, for example, food, water, food additives, chemicals and pharmaceuticals. In addition to the conventional solid compounding method, when the object to be compounded contains a certain amount of water, the compounding can be carried out as it is and dissolved in the water.
【００１８】本発明の遺伝子酸化損傷作用を抑制する方
法は、食品に低分子化核タンパク質を添加することから
なるが、その添加の形態について特に制限はない。例え
ば粉末状態で食品に振り掛けたり、混ぜ合わせたりする
ことができ、また溶液の状態で滴下添加することも可能
である。The method for suppressing the gene oxidative damage effect of the present invention comprises adding a low molecular weight nuclear protein to foods, but the mode of addition is not particularly limited. For example, the food can be sprinkled or mixed in a powder state, or can be added dropwise in a solution state.
【００１９】[0019]
【実施例】以下の例により本発明を詳細に説明するが、
本発明はこれらの例に限定されるものではない。The present invention will be described in detail with reference to the following examples.
The invention is not limited to these examples.
【００２０】実施例１：低分子化核タンパク質の製造
冷凍した鮭白子２５００ｇを解凍し、皮、筋、血管等を
除去した後、血抜きおよび水洗を行った。その後、該鮭
白子を水１０００ｍｌと共に粉砕して、プロテアーゼ
（ＮＯＶＯ社製）２．５ｇを添加し、攪拌しながら４４
〜４７℃、ｐＨ６．０〜６．３で４時間酵素処理を行っ
た。続いて、処理後の液を７０℃に昇温し、ヌクレアー
ゼ（アマノ社製）２．５ｇを添加し、攪拌しながら、ｐ
Ｈ５．０〜５．５で４時間酵素処理を行った。その後、
処理後の液を８５℃に昇温して、残存するプロテアーゼ
およびヌクレアーゼを失活させた。得られた生成液を４
０〜５０℃に冷却し、これを連続的にデカンターに送液
して清澄液を分離し、噴霧乾燥して粉体として本発明の
低分子化核タンパク質を得た。得られた粉体の性質は表
１の通りである。Example 1: Production of low molecular weight nuclear protein 2500 g of frozen salmon Shirako was thawed to remove skins, muscles, blood vessels and the like, and then blood was drained and washed with water. Then, the salmon sardine was crushed together with 1000 ml of water, and 2.5 g of protease (manufactured by NOVO) was added thereto, and the mixture was stirred while stirring.
The enzyme treatment was carried out at -47 ° C and pH 6.0-6.3 for 4 hours. Subsequently, the temperature of the treated liquid is raised to 70 ° C., 2.5 g of nuclease (manufactured by Amano) is added, and p
Enzyme treatment was performed for 4 hours at H5.0 to 5.5. afterwards,
After the treatment, the temperature of the liquid was raised to 85 ° C. to inactivate the remaining protease and nuclease. The resulting product liquid is 4
It was cooled to 0 to 50 ° C., continuously fed to a decanter to separate a clarified liquid, and spray-dried to obtain the low molecular weight nuclear protein of the present invention as a powder. The properties of the obtained powder are shown in Table 1.
【表１】 [Table 1]
【００２１】実施例２：低分子化核タンパク質による遺
伝子酸化損傷抑制作用の評価
純水を用いて、２’−デオキシグアノシン（ｄＧ）の２
００μｇ／ｍｌ溶液、化学的な酸化剤である臭素酸カリ
ウム（ＫＢｒＯ3）の５０ｍｇ／ｍｌ溶液、および実施
例１で製造した低分子化核タンパク質の１％溶液を調製
した。次いでこれらの溶液をそれぞれ５０ｍｌづつ純水
に添加し、全体が５００ｍｌとなるように希釈して試験
溶液を調製した。そうして調製した試験溶液を１０分間
放置し、放置後の該溶液中のｄＧと８ＯＨｄＧを定量し
た。また、放置の間に８００μＷ／ｃｍ2の紫外線（北
半球中緯度地域での直射日光の１０倍に相当）の照射を
行い、照射終了後、同様にｄＧと８ＯＨｄＧを定量し
た。さらに対照として、低分子化核タンパク質を添加し
ない以外は全く同じ試験溶液を調製し、同様の実験を行
った。試験結果は、８ＯＨｄＧ／ｄＧの比率で表し、該
比率が大きければ大きい程、８ＯＨｄＧがより多量に発
生したことを示し、また該比率が小さければ小さい程、
ｄＧの酸化損傷がより良好に抑制されたことを示す。結
果を表２および図１に表す。Example 2: Evaluation of inhibitory effect on gene oxidative damage by low molecular weight nuclear protein Using pure water, 2'-deoxyguanosine (dG)
A 00 μg / ml solution, a 50 mg / ml solution of potassium bromate (KBrO 3 ) which is a chemical oxidant, and a 1% solution of the reduced nuclear protein produced in Example 1 were prepared. Next, 50 ml of each of these solutions was added to pure water, and diluted so that the total amount became 500 ml to prepare a test solution. The test solution thus prepared was allowed to stand for 10 minutes, and dG and 8OHdG in the solution after standing were quantified. Also, during the standing, irradiation with ultraviolet rays of 800 μW / cm 2 (corresponding to 10 times the direct sunlight in the mid-latitude region of the northern hemisphere) was performed, and after the irradiation was completed, dG and 8OHdG were similarly quantified. Further, as a control, the same test solution was prepared except that the low molecular weight nuclear protein was not added, and the same experiment was performed. The test results are expressed by the ratio of 8OHdG / dG, and the larger the ratio is, the more the amount of 8OHdG is generated, and the smaller the ratio is,
It shows that the oxidative damage of dG was better suppressed. The results are shown in Table 2 and FIG.
【表２】
表２および図１に表す通り、本発明の低分子化核タンパ
ク質の添加は、酸化負荷が臭素酸カリウムのみの場合、
ｄＧの８ＯＨｄＧへの酸化を添加しない場合の５３％ま
でに抑制した。さらに、酸化負荷が臭素酸カリウム＋紫
外線照射の場合には、添加しない場合の僅か５％までに
抑制した。以上の結果より、本発明の低分子化核タンパ
ク質は酸化負荷が加えられた場合に遺伝子の酸化損傷を
良好に抑制することができ、しかもより強い酸化負荷が
加えられた場合にはさらに良好に酸化損傷を抑制するこ
とができることがわかる。[Table 2] As shown in Table 2 and FIG. 1, the addition of the low molecular weight nuclear protein of the present invention, when the oxidizing load is only potassium bromate,
The oxidation of dG to 8OHdG was suppressed to 53% of the case without addition. Furthermore, when the oxidizing load was potassium bromate + ultraviolet irradiation, it was suppressed to only 5% of the case where no addition was made. From the above results, the low molecular weight nuclear protein of the present invention can satisfactorily suppress oxidative damage of a gene when an oxidative load is applied, and more preferably when a stronger oxidative load is added. It can be seen that oxidative damage can be suppressed.
【００２２】[0022]
【発明の効果】本発明の遺伝子酸化損傷抑制剤は低分子
化核タンパク質からなり、遺伝子の酸化損傷を非常に良
好に抑制することができる。しかも低分子化核タンパク
質は低分子量であるため消化が容易で、水溶性であるた
め食品等への配合も簡便に行うことができる。従って、
本発明の低分子化核タンパク質は遺伝子損傷を抑制する
ためにそのまま摂取するだけでなく、確実な有効性を示
す健康食品の有効成分として用いることもできる。EFFECTS OF THE INVENTION The gene oxidative damage inhibitor of the present invention comprises a low molecular weight nuclear protein and can suppress gene oxidative damage very well. Moreover, the low molecular weight nucleoprotein has a low molecular weight and is easily digested, and since it is water-soluble, it can be easily added to foods and the like. Therefore,
The low molecular weight nuclear protein of the present invention can be used not only as it is for suppressing gene damage, but also as an active ingredient of a health food showing reliable efficacy.
【図１】 図１は、本発明の低分子化核タンパク質によ
る遺伝子酸化損傷抑制作用を評価した結果を図示するグ
ラフである。FIG. 1 is a graph showing the results of evaluation of the gene oxidation damage inhibitory effect of the reduced nuclear protein of the present invention.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｐ 39/06 Ａ６１Ｋ 37/10 Ｆターム(参考） 4B018 MD20 MD22 MD69 ME10 ME14 MF12 4C084 AA02 BA43 CA45 CA70 DC50 NA14 ZB011 ZB012 ZC022 4C086 AA01 AA02 EA16 MA02 MA04 NA14 ZB01 ZC02 4C087 AA01 AA02 BB29 CA16 CA20 MA02 NA14 ZB01 ZC02 ─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 7 Identification code FI theme code (reference) A61P 39/06 A61K 37/10 F term (reference) 4B018 MD20 MD22 MD69 ME10 ME14 MF12 4C084 AA02 BA43 CA45 CA70 DC50 NA14 ZB011 ZB012 ZC022 4C086 AA01 AA02 EA16 MA02 MA04 NA14 ZB01 ZC02 4C087 AA01 AA02 BB29 CA16 CA20 MA02 NA14 ZB01 ZC02
Claims (4)
テアーゼで処理して得られる低分子化したオリゴヌクレ
オチド／ヌクレオシドおよびオリゴペプチドを３０％以
上含むことを特徴とする水溶性遺伝子酸化損傷抑制剤。1. A water-soluble gene oxidative damage inhibitor comprising 30% or more of a low-molecular-weight oligonucleotide / nucleoside and oligopeptide obtained by treating a nuclear protein with a nuclease and a protease.
る、請求項１記載の遺伝子酸化損傷抑制剤。2. The gene oxidative damage inhibitor according to claim 1, which is produced from fish milt.
を含むことを特徴とする食品。3. A food comprising the gene oxidative damage inhibitor according to claim 1.
薬品が有する遺伝子酸化損傷作用を抑制する方法であっ
て、該食品、水、食品添加物、化学品および医薬品に遺
伝子酸化損傷抑制有効量の請求項１に記載の遺伝子酸化
損傷抑制剤を添加することからなる方法。4. A method for suppressing the gene oxidative damage effect of foods, water, food additives, chemicals and pharmaceuticals, which is effective for suppressing gene oxidative damages in the foods, water, food additives, chemicals and pharmaceuticals. A method comprising adding an amount of the gene oxidative damage inhibitor according to claim 1.
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|---|---|---|---|
| JP2002116726A JP3978716B2 (en) | 2002-04-18 | 2002-04-18 | Gene oxidative damage inhibitor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005279389A (en) * | 2004-03-29 | 2005-10-13 | Aquas Corp | Method for judging washing period of aqueous system |
| JP2007211000A (en) * | 2006-01-13 | 2007-08-23 | Nissei Bio Kk | Therapeutic agent for wound, therapeutic agent or prophylactic for bedsore or therapeutic agent for burn |
| JP2007291062A (en) * | 2006-03-31 | 2007-11-08 | Nissei Bio Kk | Compounding agent for base cosmetic, and base cosmetic |
| JP2008063315A (en) * | 2006-08-09 | 2008-03-21 | Nissei Bio Kk | Compounding ingredient for makeup product and makeup product improving texture |
| JP2009131222A (en) * | 2007-11-30 | 2009-06-18 | Maruha Nichiro Foods Inc | Nucleic acid material suitable for being mixing with drink, and method for producing the same |
| JP2016204340A (en) * | 2015-04-28 | 2016-12-08 | 国立大学法人北海道大学 | Low-density lipoprotein oxidation inhibitor |
-
2002
- 2002-04-18 JP JP2002116726A patent/JP3978716B2/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005279389A (en) * | 2004-03-29 | 2005-10-13 | Aquas Corp | Method for judging washing period of aqueous system |
| JP4676154B2 (en) * | 2004-03-29 | 2011-04-27 | アクアス株式会社 | How to determine when to wash water |
| JP2007211000A (en) * | 2006-01-13 | 2007-08-23 | Nissei Bio Kk | Therapeutic agent for wound, therapeutic agent or prophylactic for bedsore or therapeutic agent for burn |
| JP2007291062A (en) * | 2006-03-31 | 2007-11-08 | Nissei Bio Kk | Compounding agent for base cosmetic, and base cosmetic |
| JP2008063315A (en) * | 2006-08-09 | 2008-03-21 | Nissei Bio Kk | Compounding ingredient for makeup product and makeup product improving texture |
| JP2009131222A (en) * | 2007-11-30 | 2009-06-18 | Maruha Nichiro Foods Inc | Nucleic acid material suitable for being mixing with drink, and method for producing the same |
| JP2016204340A (en) * | 2015-04-28 | 2016-12-08 | 国立大学法人北海道大学 | Low-density lipoprotein oxidation inhibitor |
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| Publication number | Publication date |
|---|---|
| JP3978716B2 (en) | 2007-09-19 |
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